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University of Minnesota
1.
Oliva Chavez, Adela Sarahi.
Functional characterization of two anaplasma phagocytophilum genes required for infection of mammalian host and tick vectors, respectively.
Degree: PhD, Entomology, 2014, University of Minnesota
URL: http://hdl.handle.net/11299/162687 
► Anaplasma phagocytophilum is a tick-borne pathogen and the causative agent of Human Granulocytic Anaplasmosis (HGA). A. phagocytophilum is not transovarially transmitted from the mother to…
(more)
▼ Anaplasma phagocytophilum is a tick-borne pathogen and the causative agent of Human Granulocytic Anaplasmosis (HGA). A. phagocytophilum is not transovarially transmitted from the mother to the progeny of infected ticks and therefore needs to survive in both a mammalian and the arthropod vector in order to complete its life cycle. To adapt to different environments, A. phagocytophilum relies on differential gene expression as well as the post-translational modification of proteins. However, little is known about what A. phagocytophilum genes and enzymes are required for the infection of human or tick cells. I used random mutagenesis to generate knock-out strains of Ap for functional genomics studies. One of the mutated strains presented an insertion within the coding region of an O-methyltranferase (OMT) family 3, which affects the ability of A. phagocytophilum to infect tick cells. Studies in the function of this enzyme suggest that it is involved in the methylation of an outer membrane protein (Major Surface Protein 4), which appears to be involved in bacterial binding and entry. The second mutant presents an insertion within the coding region of a hypothetical protein in the locus APH_0906 and is unable to infect HL-60 cells and is impaired in its ability to grow in endothelial cells. Localization analysis of the protein showed that the protein is secreted into the cytoplasm and then translocated into the nucleus of host cells. Bioinformatic analyses demonstrated differences in Nuclear Localization Signals (NLSs) as well as binding residues within the protein homolog of human and non-human strains. Herein, I present the results from the functional analyses of both gene products.
Subjects/Keywords: Anaplasma phagocytophilum; Effector; Functional Genomics; Methylation
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APA (6th Edition):
Oliva Chavez, A. S. (2014). Functional characterization of two anaplasma phagocytophilum genes required for infection of mammalian host and tick vectors, respectively. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/162687
Chicago Manual of Style (16th Edition):
Oliva Chavez, Adela Sarahi. “Functional characterization of two anaplasma phagocytophilum genes required for infection of mammalian host and tick vectors, respectively.” 2014. Doctoral Dissertation, University of Minnesota. Accessed March 04, 2021.
http://hdl.handle.net/11299/162687.
MLA Handbook (7th Edition):
Oliva Chavez, Adela Sarahi. “Functional characterization of two anaplasma phagocytophilum genes required for infection of mammalian host and tick vectors, respectively.” 2014. Web. 04 Mar 2021.
Vancouver:
Oliva Chavez AS. Functional characterization of two anaplasma phagocytophilum genes required for infection of mammalian host and tick vectors, respectively. [Internet] [Doctoral dissertation]. University of Minnesota; 2014. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/11299/162687.
Council of Science Editors:
Oliva Chavez AS. Functional characterization of two anaplasma phagocytophilum genes required for infection of mammalian host and tick vectors, respectively. [Doctoral Dissertation]. University of Minnesota; 2014. Available from: http://hdl.handle.net/11299/162687
The Ohio State University
2.
Wang, Xueqi.
Expression analysis and antibody neutralization of P44 major
surface proteins of anaplasma phagocytophilum during mammalian
infection.
Degree: PhD, Veterinary Biosciences, 2006, The Ohio State University
URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1149083249
► Anaplasma phagocytophilum is an obligatory intracellular bacterium that causes human granulocytic anaplasmosis. The immunodominant polymorphic 44-kDa major surface proteins of A. phagocytophilum are encoded by…
(more)
▼ Anaplasma phagocytophilum is an obligatory
intracellular bacterium that causes human granulocytic
anaplasmosis. The immunodominant polymorphic 44-kDa major surface
proteins of A.
phagocytophilum are encoded by more than 80 p44
paralogs. Although diverse P44 species are shown to be expressed,
what drives the P44 antigenic variation in mammals is unknown.
Objectives of this study were to 1) characterize the temporal and
dynamic expression of p44 genes during the infection of horses,
immunocompetent and immunocompromised mice, and in cell culture, 2)
examine expression of genes involved in homologous recombination
and polycistronic expression of the p44-expression locus, and 3)
elucidate P44 antibody neutralization mechanisms in vitro. First,
our results showed rapid switch-off of the initial dominant
transcript p44-18 occurred in the blood of horses during the
logarithmic increase of bacteria growth. Each of the subsequently
dominant p44 transcript species was phylogenetically dissimilar
from p44-18. When A.
phagocytophilum was preincubated with infected
horse plasma, the dominance of the p44-18 transcript was rapidly
suppressed in vitro and most of the newly emerged p44 transcript
species were previously undetected in this horse. Second, we
demonstrated two monoclonal antibodies recognize bacterial
surface-exposed epitopes of naturally folded P44 proteins and
mapped these epitopes to specific peptide sequences. These results
indicated that antibodies directed to certain epitopes of P44
proteins have a critical role in inhibiting A.
phagocytophilum
infection. Third, immunocompetent mice cleared A.
phagocytophilum
infection by 3 weeks post inoculation (p.i.), whereas A.
phagocytophilum persisted at least two months in C3H/HeJ (TLR4
deficient) and SCID mice. The development of antibodies against P44
N-terminus region was delayed in C3H/HeJ mice. Of seven genes
involved in homologous recombination, only recA mRNA level in A.
phagocytophilum was significantly greater in mice than in cell
culture. Fourth, an electrophoretic migration shift assay showed a
specific shift of the promoter region of tr1 in the p44-expression
locus upon incubation with A.
phagocytophilum lysate. These studies
are expected to facilitate the understanding of the mechanisms of
P44 antigenic variation: interplay between bacterial recombination
and transcriptional regulation and immunoclearance, and a new
approach in designing vaccine candidate antigens for the control of
HGA.
Advisors/Committee Members: Rikihisa, Yasuko (Advisor).
Subjects/Keywords: Anaplasma phagocytophilum; p44
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APA (6th Edition):
Wang, X. (2006). Expression analysis and antibody neutralization of P44 major
surface proteins of anaplasma phagocytophilum during mammalian
infection. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1149083249
Chicago Manual of Style (16th Edition):
Wang, Xueqi. “Expression analysis and antibody neutralization of P44 major
surface proteins of anaplasma phagocytophilum during mammalian
infection.” 2006. Doctoral Dissertation, The Ohio State University. Accessed March 04, 2021.
http://rave.ohiolink.edu/etdc/view?acc_num=osu1149083249.
MLA Handbook (7th Edition):
Wang, Xueqi. “Expression analysis and antibody neutralization of P44 major
surface proteins of anaplasma phagocytophilum during mammalian
infection.” 2006. Web. 04 Mar 2021.
Vancouver:
Wang X. Expression analysis and antibody neutralization of P44 major
surface proteins of anaplasma phagocytophilum during mammalian
infection. [Internet] [Doctoral dissertation]. The Ohio State University; 2006. [cited 2021 Mar 04].
Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1149083249.
Council of Science Editors:
Wang X. Expression analysis and antibody neutralization of P44 major
surface proteins of anaplasma phagocytophilum during mammalian
infection. [Doctoral Dissertation]. The Ohio State University; 2006. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1149083249
3.
Parra, Andréa Cristina.
Investigação diagnóstica de doença concomitante babesiose e anaplasmose em rebanho eqüino, por técnicas de Nested PCR e c - ELISA ou ELISA indireto.
Degree: PhD, Clínica Veterinária, 2009, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/10/10136/tde-10022011-152248/
;
► Em função da proximidade cada vez maior entre o cavalo e o homem, é de extrema importância ter conhecimentos das doenças que acometem os equinos,…
(more)
▼ Em função da proximidade cada vez maior entre o cavalo e o homem, é de extrema importância ter conhecimentos das doenças que acometem os equinos, que por ventura, podem acometer seres humanos. Dentre muitas doenças, pode-se citar duas, que promovem grandes perdas econômicas aos rebanhos eqüinos, tanto no tratamento desses rebanhos, como com a morte dos mesmos, dificultando a importação e exportação de animais: a babesiose e a erliquiose (anaplasmose), que podem estar ou não associadas, acometendo um animal, concomitantemente. A presente pesquisa teve como objetivo investigar e diagnosticar doença concomitante babesiose (por Babesia equi ou Theileria equi) e Erliquiose (por Erliquia equi ou Anaplasma phagocytophilum), no estado de São Paulo, em rebanhos eqüinos, utilizando as técnicas de Nested PCR (Nested polymerase chain reaction reação em cadeia pela polimerase para diagnóstico de T. equi e A. phagocytophilum) e c-ELISA (competitive enzyme-linked immunosorbent assay para diagnóstico de T. equi) ou ELISA indireto (para diagnóstico de A. phagocytophilum) e comparar os resultados obtidos nas diferentes técnicas em 250 amostras de eqüino (sangue total e soro). Como resultado, obteve-se 38,4%, 46% e 36% de positividade, respectivamente, nos testes de pesquisa de hematozoário, c-ELISA e Nested PCR para Theileria equi e 0%, 3% e 0% de positividade, respectivamente, nos testes de pesquisa de hemoparasita, ELISA indireto e Nested PCR para Anaplasma phagocytophilum, não sendo observada a co-infecção de Babesiose e Anaplasmose no rebanho estudo
Due to the increasing proximity between horse and man, it is of extreme importance to understand the diseases that affect horses which by chance may affect humans. Among many diseases, babesiosis and ehrlichiosis (anaplasmosis) promote high economic losses to horses herds in consequence of costs of treatment and also death, making it difficult to import and export animals: They can or not be linked affecting an animal at the same time. This study aimed to investigate and diagnose concomitant babesiosis (Babesia equi and Theileria equi) and ehrlichiosis (for ehrlichia equipment or Anaplasma phagocytophilum) in equine herds of the state of Sao Paulo, using the techniques of Nested PCR (Nested polymerase chain reaction for the diagnosis of T. equi and A. phagocytophilum) and c-ELISA (competitive enzyme-linked immunosorbent assay for diagnosis of T. equi) or ELISA (for diagnosis of A. phagocytophilum). Also to compare results obtained in these different techniques in 250 samples of horse (whole blood and serum). Results showed 38.4%, 46% and 36% positivity, respectively, in tests for the detection of Theileria equi through hematozoan, c-ELISA and Nested PCR and 0%, 3% and 0% positivity, respectively, in tests for the detection of Anaplasma phagocytophilum through blood parasites, indirect ELISA and Nested PCR. It was not observed co-infection Babesiosis and anaplasmosis in the herd study
Advisors/Committee Members: Fernandes, Wilson Roberto.
Subjects/Keywords: Anaplasma phagocytophilum; Anaplasma phagocytophilum; Nested PCR; Nested PCR; Theileria equi; Theileria equi; ELISA; ELISA; Equine; Equino
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APA (6th Edition):
Parra, A. C. (2009). Investigação diagnóstica de doença concomitante babesiose e anaplasmose em rebanho eqüino, por técnicas de Nested PCR e c - ELISA ou ELISA indireto. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/10/10136/tde-10022011-152248/ ;
Chicago Manual of Style (16th Edition):
Parra, Andréa Cristina. “Investigação diagnóstica de doença concomitante babesiose e anaplasmose em rebanho eqüino, por técnicas de Nested PCR e c - ELISA ou ELISA indireto.” 2009. Doctoral Dissertation, University of São Paulo. Accessed March 04, 2021.
http://www.teses.usp.br/teses/disponiveis/10/10136/tde-10022011-152248/ ;.
MLA Handbook (7th Edition):
Parra, Andréa Cristina. “Investigação diagnóstica de doença concomitante babesiose e anaplasmose em rebanho eqüino, por técnicas de Nested PCR e c - ELISA ou ELISA indireto.” 2009. Web. 04 Mar 2021.
Vancouver:
Parra AC. Investigação diagnóstica de doença concomitante babesiose e anaplasmose em rebanho eqüino, por técnicas de Nested PCR e c - ELISA ou ELISA indireto. [Internet] [Doctoral dissertation]. University of São Paulo; 2009. [cited 2021 Mar 04].
Available from: http://www.teses.usp.br/teses/disponiveis/10/10136/tde-10022011-152248/ ;.
Council of Science Editors:
Parra AC. Investigação diagnóstica de doença concomitante babesiose e anaplasmose em rebanho eqüino, por técnicas de Nested PCR e c - ELISA ou ELISA indireto. [Doctoral Dissertation]. University of São Paulo; 2009. Available from: http://www.teses.usp.br/teses/disponiveis/10/10136/tde-10022011-152248/ ;
4.
Dugat, Thibaud.
L’anaplasmose granulocytaire bovine en France : caractérisation du premier génome d’Anaplasma phagocytophilum provenant d’un bovin et étude de la circulation des souches de ruminants par MLVA : Bovine granulocytic anaplasmosis in France : characterization of first available bovine Anaplasma phagocytophilum genome and epidemiologic study of ruminants strains by MLVA.
Degree: Docteur es, Sciences du vivant, 2014, Université Paris-Est
URL: http://www.theses.fr/2014PEST1188
► Anaplasma phagocytophilum est une alpha-protéobactérie, parasite intracellulaire stricte, à localisation intra-granulocytaire et principalement vectorisée par des tiques du genre Ixodes. Elle est notamment l'agent de…
(more)
▼ Anaplasma phagocytophilum est une alpha-protéobactérie, parasite intracellulaire stricte, à localisation intra-granulocytaire et principalement vectorisée par des tiques du genre Ixodes. Elle est notamment l'agent de l'anaplasmose granulocytaire bovine, ou Tick-borne fever, une maladie provoquant d'importantes pertes économiques chez les bovins en Europe. Cette bactérie, peut également infecter un large spectre d'hôtes, tels que des ruminants sauvages ou des rongeurs. Cependant, l'épidémiologie de l'infection par A. phagocytophilum est encore mal connue. Le(s) réservoir(s) des souches de ruminants domestiques en Europe n'est/ne sont notamment pas identifié(s) à l'heure actuelle. Il est donc nécessaire d'approfondir nos connaissances dans ce domaine, notamment afin de lutter plus efficacement contre l'atteinte des bovins. L'objectif de cette thèse était de caractériser la diversité génétique des variants d'A. phagocytophilum circulant chez les ruminants en France. Pour cela, nous avons, dans un premier temps, étudié la circulation des souches d'A. phagocytophilum au sein des ruminants sauvages et domestiques en développant et en utilisant une technique MLVA (Multiple-Locus Variable-number tandem repeat Analysis). Cela nous a permis de suspecter fortement l'existence d'au moins deux cycles épidémiologiques impliquant des variants d'A. phagocytophilum présents chez les ruminants en France. Le premier pourrait impliquer le cerf comme espèce réservoir et les ruminants domestiques (entre autres) comme espèces sensibles, alors que le second impliquerait le chevreuil comme réservoir, sans impact significatif chez les bovins. Dans un deuxième temps, après avoir développé une technique de séquence capture pour A. phagocytophilum, nous avons séquencé et caractérisé le premier génome de cette bactérie issu d'un bovin. La comparaison de ce génome aux neuf autres génomes actuellement disponibles a permis d'identifier quatre protéines uniquement présentes chez la souche bovine, et neuf uniquement chez les deux souches de ruminants domestiques étudiés, ce qui amène à envisager leur implication potentielle dans le tropisme d'hôte de ces souches
Anaplasma phagocytophilum is an obligate intracellular alpha-proteobacterium mainly transmitted by Ixodes ticks. In domestic ruminants, it is the causative agent of tick-borne fever, which causes significant economic losses in Europe. It also infects a large range of hosts, including wild ruminants and rodents. The epidemiology of A. phagocytophilum is not yet fully understood. For example, the reservoir host(s) for European domestic ruminant strains has/have not been identified to date, which doesn't facilitate control of cattle infection. Our objective was to explore the genetic diversity of A. phagocytophilum obtained from ruminants in France. For this purpose, we first studied the circulation of this pathogen in domestic and wild ruminants, using a new MLVA technique. Our results potentially reveal the existence of at least two different epidemiological transmission cycles of A.…
Advisors/Committee Members: Haddad, Nadia (thesis director).
Subjects/Keywords: Anaplasma phagocytophilum; Épidémiologie moléculaire; Génomique; Mlva; Ruminants; Tropisme d'hôtes; Anaplasma phagocytophilum; Ruminants; Mlva; Molecular epidemiology; Genomic; Host tropisme
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APA ·
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APA (6th Edition):
Dugat, T. (2014). L’anaplasmose granulocytaire bovine en France : caractérisation du premier génome d’Anaplasma phagocytophilum provenant d’un bovin et étude de la circulation des souches de ruminants par MLVA : Bovine granulocytic anaplasmosis in France : characterization of first available bovine Anaplasma phagocytophilum genome and epidemiologic study of ruminants strains by MLVA. (Doctoral Dissertation). Université Paris-Est. Retrieved from http://www.theses.fr/2014PEST1188
Chicago Manual of Style (16th Edition):
Dugat, Thibaud. “L’anaplasmose granulocytaire bovine en France : caractérisation du premier génome d’Anaplasma phagocytophilum provenant d’un bovin et étude de la circulation des souches de ruminants par MLVA : Bovine granulocytic anaplasmosis in France : characterization of first available bovine Anaplasma phagocytophilum genome and epidemiologic study of ruminants strains by MLVA.” 2014. Doctoral Dissertation, Université Paris-Est. Accessed March 04, 2021.
http://www.theses.fr/2014PEST1188.
MLA Handbook (7th Edition):
Dugat, Thibaud. “L’anaplasmose granulocytaire bovine en France : caractérisation du premier génome d’Anaplasma phagocytophilum provenant d’un bovin et étude de la circulation des souches de ruminants par MLVA : Bovine granulocytic anaplasmosis in France : characterization of first available bovine Anaplasma phagocytophilum genome and epidemiologic study of ruminants strains by MLVA.” 2014. Web. 04 Mar 2021.
Vancouver:
Dugat T. L’anaplasmose granulocytaire bovine en France : caractérisation du premier génome d’Anaplasma phagocytophilum provenant d’un bovin et étude de la circulation des souches de ruminants par MLVA : Bovine granulocytic anaplasmosis in France : characterization of first available bovine Anaplasma phagocytophilum genome and epidemiologic study of ruminants strains by MLVA. [Internet] [Doctoral dissertation]. Université Paris-Est; 2014. [cited 2021 Mar 04].
Available from: http://www.theses.fr/2014PEST1188.
Council of Science Editors:
Dugat T. L’anaplasmose granulocytaire bovine en France : caractérisation du premier génome d’Anaplasma phagocytophilum provenant d’un bovin et étude de la circulation des souches de ruminants par MLVA : Bovine granulocytic anaplasmosis in France : characterization of first available bovine Anaplasma phagocytophilum genome and epidemiologic study of ruminants strains by MLVA. [Doctoral Dissertation]. Université Paris-Est; 2014. Available from: http://www.theses.fr/2014PEST1188
5.
Le Corre, Anne-Claire.
Approche multi-échelles (élevage, cellule, -omique) des mécanismes de transmission inter-espèces d’Anaplasma phagocytophilum et de sa circulation chez les bovins : Study of inter-species transmission and intra-cattle herd circulation of Anaplasma phagocytophilum using a multi-scale approach (herd, cell, -omic).
Degree: Docteur es, Sciences de la vie et de la santé, 2017, Université Paris-Est
URL: http://www.theses.fr/2017PESC1073
► Anaplasma phagocytophilum est une alpha-protéobactérie à multiplication intracellulaire stricte transmise par les tiques du genre Ixodes sp. Responsable notamment des anaplasmoses granulocytaires bovine et humaine,…
(more)
▼ Anaplasma phagocytophilum est une alpha-protéobactérie à multiplication intracellulaire stricte transmise par les tiques du genre Ixodes sp. Responsable notamment des anaplasmoses granulocytaires bovine et humaine, elle peut également infecter de nombreux mammifères, tels les ruminants sauvages ou les rongeurs. En Europe, plusieurs cycles, encore mal connus, semblent coexister et impliquer des hôtes (réservoirs et victimes) différents. Cela semble être en particulier le cas pour les souches bovines et humaines, conduisant à supposer que les souches bovines ne seraient pas zoonotiques. Du fait de sa localisation intracellulaire in vivo, et plus particulièrement au sein des polynucléaires neutrophiles des hôtes vertébrés, la culture d’A.
phagocytophilum au laboratoire expose à d’importantes difficultés méthodologiques. De ce fait, la compréhension des relations entre la bactérie et ses hôtes (mammifères et tiques vectrices) n’a conduit à ce jour qu’à un nombre restreint de publications. Cette thèse s’intéresse à ces interactions, que j’ai abordées sciemment à différents niveaux. Dans un premier temps, l’étude épidémiologique menée en troupeau bovin nous a permis de mettre en évidence une diversité génétique importante parmi les souches y circulant, et nous a amenés à formuler l’hypothèse que les bovins pourraient jouer un rôle en tant que réservoirs de l’infection. Différents mécanismes, notamment l’échappement à la réponse immunitaire de l’hôte, l’absence de protection croisée entre souches et peut-être la sanctuarisation de l’infection dans des cellules niches, permettraient d’expliquer ce rôle de réservoir. L’étude de l’infection des cellules endothéliales, effectuée afin d’explorer leur rôle en tant que cellules niches d’une part et leur implication dans la barrière d’espèce d’autre part, a conduit à envisager que ces cellules pourraient permettre le passage des bactéries vers le courant sanguin, mais a priori pas leur multiplication. Afin d’amorcer l’exploration de la réponse transcriptomique d’A.
phagocytophilum lors du changement d’hôte (vertébré vs invertébré), nous avons soumis des cultures de cellules de tiques infectées à un choc thermique, ce qui nous a permis de suggérer qu’un nombre restreint de mécanismes transcriptomiques est mis en jeu en réponse au choc thermique induit par le changement d’hôte vertébré/invertébré. Néanmoins, la capacité d’A.
phagocytophilum à répondre à un stress thermique plus important est bien maintenue. Les protéines que nous avons identifiées comme les plus différentiellement exprimées au cours de cette étude pourraient s’avérer jouer un rôle préférentiel dans l’infection du vecteur ou du mammifère. La technique du double-hybride en système de levure, expérimentée dans la dernière partie de ce travail, et qui nous a permis de mettre en évidence trois interacteurs pour APH_0032, protéine de la membrane vacuolaire, pourrait s’avérer intéressante pour étudier les interactions de ces protéines vis-à-vis des banques d’ADNc de vertébré et de tique, mais aussi pour explorer le tropisme…
Advisors/Committee Members: Boulouis, Henri-Jean (thesis director), Haddad, Nadia (thesis director).
Subjects/Keywords: Anaplasma phagocytophilum; Homme; Bovin; Cellules hôtes; Barrière d'espèce; Réservoir; Anaplasma phagocytophilum; Human; Cattle; Host cells; Species barrier; Reservoir
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APA ·
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APA (6th Edition):
Le Corre, A. (2017). Approche multi-échelles (élevage, cellule, -omique) des mécanismes de transmission inter-espèces d’Anaplasma phagocytophilum et de sa circulation chez les bovins : Study of inter-species transmission and intra-cattle herd circulation of Anaplasma phagocytophilum using a multi-scale approach (herd, cell, -omic). (Doctoral Dissertation). Université Paris-Est. Retrieved from http://www.theses.fr/2017PESC1073
Chicago Manual of Style (16th Edition):
Le Corre, Anne-Claire. “Approche multi-échelles (élevage, cellule, -omique) des mécanismes de transmission inter-espèces d’Anaplasma phagocytophilum et de sa circulation chez les bovins : Study of inter-species transmission and intra-cattle herd circulation of Anaplasma phagocytophilum using a multi-scale approach (herd, cell, -omic).” 2017. Doctoral Dissertation, Université Paris-Est. Accessed March 04, 2021.
http://www.theses.fr/2017PESC1073.
MLA Handbook (7th Edition):
Le Corre, Anne-Claire. “Approche multi-échelles (élevage, cellule, -omique) des mécanismes de transmission inter-espèces d’Anaplasma phagocytophilum et de sa circulation chez les bovins : Study of inter-species transmission and intra-cattle herd circulation of Anaplasma phagocytophilum using a multi-scale approach (herd, cell, -omic).” 2017. Web. 04 Mar 2021.
Vancouver:
Le Corre A. Approche multi-échelles (élevage, cellule, -omique) des mécanismes de transmission inter-espèces d’Anaplasma phagocytophilum et de sa circulation chez les bovins : Study of inter-species transmission and intra-cattle herd circulation of Anaplasma phagocytophilum using a multi-scale approach (herd, cell, -omic). [Internet] [Doctoral dissertation]. Université Paris-Est; 2017. [cited 2021 Mar 04].
Available from: http://www.theses.fr/2017PESC1073.
Council of Science Editors:
Le Corre A. Approche multi-échelles (élevage, cellule, -omique) des mécanismes de transmission inter-espèces d’Anaplasma phagocytophilum et de sa circulation chez les bovins : Study of inter-species transmission and intra-cattle herd circulation of Anaplasma phagocytophilum using a multi-scale approach (herd, cell, -omic). [Doctoral Dissertation]. Université Paris-Est; 2017. Available from: http://www.theses.fr/2017PESC1073
Swedish University of Agricultural Sciences
6.
Franzén, Peter.
On Anaplasma phagocytophilum in horses.
Degree: 2008, Swedish University of Agricultural Sciences
URL: http://pub.epsilon.slu.se/1859/
► Equine Granulocytic Anaplasmosis (EGA) is an acute tick-borne infection caused by Anaplasma phagocytophilum. The bacterium can infect various animal species and humans. Persistence of infection…
(more)
▼ Equine Granulocytic Anaplasmosis (EGA) is an acute tick-borne infection caused by Anaplasma phagocytophilum. The bacterium can infect various animal species and humans. Persistence of infection is documented in ruminants and is shown experimentally in dogs, but it is unknown whether it occurs in horses or if EGA induces longer-term clinical changes in horses. This thesis includes results and analyses of a serosurvey of the exposure level and association with clinical disease (excluding acute EGA) in over 2000 horses throughout Sweden. It also includes an experimental infection study of six horses using a Swedish isolate of the bacterium. Overall 17% of the horses were seropositive with large geographical variations. However, seropositivity was not statistically associated with any increase in clinical sign or disease in the animals. In the experimental study acute disease was readily induced and the clinical disease was similar to EGA described from USA. Laboratory data showed that the PCR test had the widest diagnostic window for EGA with positive signals days before onset of clinical signs. Clinically, diagnostic inclusions (morulae) in leukocytes in blood smears were visible first after a few days into clinical disease and lasted shorter time than did the PCR signal. One horse died suddenly 2 days into the acute disease, with post mortem changes of general vasculitis and hyaline thrombi in the kidneys suggesting disseminative intravascular coagulation (DIC). This was the first documented case of death attributed probably soley to EGA. The remaining horses were closely monitored for more than 3 months after spontaneous recovery from EGA. Some of the horses became transiently PCR positive during this period, mainly close in time after selected interventions to mimic stress. However no detectable clinical abnormalities were found during this follow-up period. At post mortem there were no macroscopic or microscopic changes in any of the tissues that could be associated with persistence of infection. In conclusion this thesis shows that for EGA; seropositive horses in Sweden are common but do not have increased levels of clinical disease; PCR provides the earliest laboratory diagnosis for acute EGA-infection; sudden death due to EGA can occur; and the organism can persist in some horses for up to 3 months post recovery but was clinically and pathologically silent.
Subjects/Keywords: horses; anaplasma; bacterioses; pcr; clinical trials; immunology; Anaplasma phagocytophilum; horse; serosurvey; PCR; experimental infection; persistence.
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APA (6th Edition):
Franzén, P. (2008). On Anaplasma phagocytophilum in horses. (Doctoral Dissertation). Swedish University of Agricultural Sciences. Retrieved from http://pub.epsilon.slu.se/1859/
Chicago Manual of Style (16th Edition):
Franzén, Peter. “On Anaplasma phagocytophilum in horses.” 2008. Doctoral Dissertation, Swedish University of Agricultural Sciences. Accessed March 04, 2021.
http://pub.epsilon.slu.se/1859/.
MLA Handbook (7th Edition):
Franzén, Peter. “On Anaplasma phagocytophilum in horses.” 2008. Web. 04 Mar 2021.
Vancouver:
Franzén P. On Anaplasma phagocytophilum in horses. [Internet] [Doctoral dissertation]. Swedish University of Agricultural Sciences; 2008. [cited 2021 Mar 04].
Available from: http://pub.epsilon.slu.se/1859/.
Council of Science Editors:
Franzén P. On Anaplasma phagocytophilum in horses. [Doctoral Dissertation]. Swedish University of Agricultural Sciences; 2008. Available from: http://pub.epsilon.slu.se/1859/
Oklahoma State University
7.
Reppert, Emily Jean.
Characterization of Tick Feeding Sites on Sheep Experimentally Infected with the Human Ny-18 Isolate of Anaplasma Phagocytophilum.
Degree: Veterinary Biomedical Sciences, 2014, Oklahoma State University
URL: http://hdl.handle.net/11244/15085
► Anaplasma phagocytophilum, first identified as a pathogen of ruminants in Europe, has more recently been recognized as an emerging tick-borne pathogen of humans in the…
(more)
▼ Anaplasma phagocytophilum, first identified as a pathogen of ruminants in Europe, has more recently been recognized as an emerging tick-borne pathogen of humans in the U.S. and Europe. A.
phagocytophilum is transmitted primarily by ticks of the genus Ixodes. Our laboratory recently developed a sheep model for study of the host/ tick/pathogen interactions of the human NY-18 isolate of A.
phagocytophilum. In this model, sheep became infected with the pathogen within 14 days after inoculation but did not exhibit clinical signs and infected morulae were rarely seen in stained blood smears. However, when ticks were allowed to feed on the infected sheep, they readily acquired A.
phagocytophilum infection, and 80% to 100% of the tick salivary glands and guts were confirmed by PCR to be infected after a 2- to 4-day feeding period. In this research we examined tick feeding sites to determine the source of A.
phagocytophilum infection for the ticks using PCR and immunohistochemistry (IHC). Postmortem skin biospies were taken directly below tick feeding sites, fixed in buffered formalin and embedded in paraffin. IHC was done using antibodies against recombinant major surface protein 4 (MSP4) that were indirectly labeled with fluorescein (FA) or peroxidase-antiperoxidase (PAP) and then examined with confocal or light microscopy. Expression of immune response genes, shown previously to be differentially regulated in response to A.
phagocytophilum infection in sheep, was determined by qRT-PCR in blood and skin biopsies. Variable expression of these genes was observed in tick and non-tick feeding sites of infected and uninfected sheep. Granuloctyes infected with A.
phagocytophilum were detected in skin biopsies by both IHC methods. Tick feeding appears to attract infected neutrophils and therefore contributes to the exposure and infection of ticks with A.
phagocytophilum after short feeding periods.
Advisors/Committee Members: Kocan, Katherine M. (advisor), Streeter, Robert N. (committee member), Fuente, Jose de la (committee member), Blouin, Edmour (committee member).
Subjects/Keywords: anaplasma phagocytophilum; immunocytochemistry; ixodes scapularis; tick bite site
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APA ·
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MLA ·
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CSE |
Export
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APA (6th Edition):
Reppert, E. J. (2014). Characterization of Tick Feeding Sites on Sheep Experimentally Infected with the Human Ny-18 Isolate of Anaplasma Phagocytophilum. (Thesis). Oklahoma State University. Retrieved from http://hdl.handle.net/11244/15085
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Reppert, Emily Jean. “Characterization of Tick Feeding Sites on Sheep Experimentally Infected with the Human Ny-18 Isolate of Anaplasma Phagocytophilum.” 2014. Thesis, Oklahoma State University. Accessed March 04, 2021.
http://hdl.handle.net/11244/15085.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Reppert, Emily Jean. “Characterization of Tick Feeding Sites on Sheep Experimentally Infected with the Human Ny-18 Isolate of Anaplasma Phagocytophilum.” 2014. Web. 04 Mar 2021.
Vancouver:
Reppert EJ. Characterization of Tick Feeding Sites on Sheep Experimentally Infected with the Human Ny-18 Isolate of Anaplasma Phagocytophilum. [Internet] [Thesis]. Oklahoma State University; 2014. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/11244/15085.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Reppert EJ. Characterization of Tick Feeding Sites on Sheep Experimentally Infected with the Human Ny-18 Isolate of Anaplasma Phagocytophilum. [Thesis]. Oklahoma State University; 2014. Available from: http://hdl.handle.net/11244/15085
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Georgia
8.
Tate, Cynthia.
Course of infection, pathology, and biology of Anaplasma phagocytophilum and a previously undescribed Anaplasma sp. in white-tailed deer.
Degree: 2014, University of Georgia
URL: http://hdl.handle.net/10724/22528
► The goals of this research were to better understand the course of infection, pathology, and biology of Anaplasma organisms harbored by white-tailed deer (WTD; Odocoileus…
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▼ The goals of this research were to better understand the course of infection, pathology, and biology of Anaplasma organisms harbored by white-tailed deer (WTD; Odocoileus virginianus). The study comprised two objectives: 1) to evaluate the
ability of WTD to serve as hosts of and as sentinels for A. phagocytophilum, and 2) to characterize an undescribed Anaplasma sp. of WTD. Four WTD were inoculated with a human isolate of A. phagocytophilum and two served as negative controls. All 4 deer
developed antibody titers • 64, three circulated organisms in blood as detected by polymerase chain reaction (PCR) for over two weeks, one had PCR-positive bone marrow at 66 days, but none appeared clinically ill. These data confirm that WTD are
susceptible to infection with a human isolate of A. phagocytophilum and suggest that WTD may maintain circulating organisms for a sufficient time to infect ticks. Their susceptibility and immunologic response render WTD suitable candidates as natural
sentinels. To accomplish the second goal, six WTD were inoculated with either a culture isolate of the Anaplasma sp. or with infected deer blood. All six became persistently infected, as determined by PCR of blood, but none appeared clinically ill. Light
microscopy revealed tiny, dark, spherical structures in platelets of infected deer and electron microscopy demonstrated membrane-bound, Anaplasma-like organisms. Immunohistochemistry and in situ hybridization localized to Anaplasma sp. organisms in
platelets and Anaplasma sp. DNA was amplified from purified platelets. Five deer developed antibodies reactive to Anaplasma sp. antigen and remained seroreactive for ten or more weeks. Slight antigenic cross-reactivity occurred among this Anaplasma sp.,
A. phagocytophilum, and A. marginale. Phylogenetic analysis demonstrated that a partial gltA gene sequence amplified from this Anaplasma sp. was closest to A. platys gltA sequence, which is concordant with previous analyses of the Anaplasma sp. 16S and
GroESL genes. Two attempts to transmit Anaplasma sp. to deer via Amblyomma americanum, a suspected tick vector, were unsuccessful. The ultrastructural, antigenic, and phyogenetic characteristics of this organism confirm that it is a distinct species of
Anaplasma which persistently infects WTD platelets. The name Anaplasma odocoilei is proposed for this new species.
Subjects/Keywords: Amblyomma americanum; Anaplasma odocoilei; Anaplasma phagocytophilum; Experimental infection; Lone star tick; Odocoileus virginianus; Platelet; Sentinel; White-tailed deer
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APA (6th Edition):
Tate, C. (2014). Course of infection, pathology, and biology of Anaplasma phagocytophilum and a previously undescribed Anaplasma sp. in white-tailed deer. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/22528
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Tate, Cynthia. “Course of infection, pathology, and biology of Anaplasma phagocytophilum and a previously undescribed Anaplasma sp. in white-tailed deer.” 2014. Thesis, University of Georgia. Accessed March 04, 2021.
http://hdl.handle.net/10724/22528.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Tate, Cynthia. “Course of infection, pathology, and biology of Anaplasma phagocytophilum and a previously undescribed Anaplasma sp. in white-tailed deer.” 2014. Web. 04 Mar 2021.
Vancouver:
Tate C. Course of infection, pathology, and biology of Anaplasma phagocytophilum and a previously undescribed Anaplasma sp. in white-tailed deer. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/10724/22528.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Tate C. Course of infection, pathology, and biology of Anaplasma phagocytophilum and a previously undescribed Anaplasma sp. in white-tailed deer. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/22528
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Helsinki
9.
Kapiainen, Suvi.
The prevalence of Anaplasma phagocytophilum in Finnish dogs.
Degree: Department of Equine and Small Animal Medicine; Helsingin yliopisto, Eläinlääketieteellinen tiedekunta, Kliinisen hevos- ja pieneläinlääketieteen osasto; Helsingfors universitet, Veterinärmedicinska fakulteten, Avdelningen för klinisk häst- och smådjursmedicin, 2016, University of Helsinki
URL: http://hdl.handle.net/10138/165474
► Puutiaisvälitteisten tautien esiintyvyydestä suomalaisissa koirissa on vain vähän tutkimustietoa. Maailmanlaajuisesti nämä taudit, anaplasmoosi yhtenä niistä, ovat suhteellisen laajalti tutkittuja. Joissain Euroopan maissa tauti on yleistynyt…
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▼ Puutiaisvälitteisten tautien esiintyvyydestä suomalaisissa koirissa on vain vähän tutkimustietoa. Maailmanlaajuisesti nämä taudit, anaplasmoosi yhtenä niistä, ovat suhteellisen laajalti tutkittuja. Joissain Euroopan maissa tauti on yleistynyt ja se on myös levinnyt ylemmäs pohjoiseen Eurooppaan. Tutkimuksen tarkoituksena oli selvittää Anaplasma phagocytophilum -bakteerin esiintyvyyttä suomalaisissa koirissa, bakteremian suhdetta vasta-aineisiin ja arvioida altistumisen riskitekijöitä. Hypoteesejä olivat, että bakteeri on alidiagnosoitu Suomessa, Etelä-Suomessa on enemmän infektioita sekä vasta-ainepositiivisia ja että metsästyskoirat ovat alttiimpia tartunnalle kuin muut koirat.
Materiaalina oli 390 koiran verinäytteet (kokoveri ja seerumi), joista 50 oli metsästyskoirien. Näytteitä kerättiin koko Suomesta. Kokoverinäytteistä eristettiin DNA, joka tutkittiin reaaliaikaisen PCR:n avulla bakteremian todentamiseksi. Vasta-ainepositiivisuus tutkittiin veren seeruminäytteistä kaupallisella SNAP 4Dx-testillä.
Anaplasma phagocytophilumin vasta-aine-esiintyvyys suomalaisissa koirissa oli 4,6% (18/390) ja metsästyskoirissa 4,0% (2/50). Alueellisesti Ahvenanmaalla oli eniten positiivisia koiria, 45% (9/20). Pienet koirat olivat useammin vasta-ainepositiivisia kuin suuret. Vain kaksi koiraa oli PCR-positiivisia eli vain kahdella oli infektio (2/390). Molemmat näistä koirista olivat vasta-ainetestissä negatiivisia.
Tämän tutkimuksen mukaan koiran anaplasmoosi on tärkeä puutiaisvälitteinen tauti suomalaisissa koirissa. Tulosten mukaan koirat altistuvat tartunnalle erityisesti Ahvenanmaalla ja pienet koirat voivat olla alttiimpia tartunnalle. Muualla maassa esiintyvyys oli pienempi, mutta on mahdollista, että se kasvaa tulevaisuudessa ilmaston lämpenemisen vuoksi ja puutiaisten levitessä pohjoisempaan.
Varsinaisen infektion esiintyvyys oli pieni. Tästä syystä PCR-positiivisuuden ja riskitekijöiden välisiä suhteita ei voitu analysoida. Aihetta tulee tutkia tarkemmin ja tulevissa tutkimuksissa tulee ottaa laajemmasta joukosta koiria näytteitä, jotta saadaan enemmän tietoa infektioiden ja vasta-ainepositiivisuuksien suhteista sekä parempi ymmärrys Anaplasma phagocytophilum-infektioihin liittyvistä riskitekijöistä koiria ja myös ihmisiä ajatellen.
Only little data is available on seroprevalence of tick borne diseases in Finnish dogs. Worldwide these diseases, anaplasmosis being one of them, are relatively widely studied. In some European countries the disease has become more common than earlier and it has spread more towards North just in the last few years. The aim of this study was to investigate the seroprevalence of Anaplasma phagocytophilum –bacterium in Finnish dogs, the PCR-positivity in relation with seropositivity and the risk factors for exposure to a vector borne pathogen. The hypotheseis were that Anaplasma phagocytophilum is underdiagnosed in Finland, there are more Anaplasma phagocytophilum -infections and seropositive dogs in the southern parts of Finland and that hunting dogs are more susceptible for Anaplasma…
Subjects/Keywords: anaplasmoosi; esiintyvyys; anaplasma phagocytophilum; anaplasmosis; dog; seroprevalence; koira; Pieneläinten sisätautioppi; Smådjurs internmedicin; Small Animal Internal Medicine; anaplasmoosi; esiintyvyys; anaplasma phagocytophilum; anaplasmosis; dog; seroprevalence
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APA ·
Chicago ·
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CSE |
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APA (6th Edition):
Kapiainen, S. (2016). The prevalence of Anaplasma phagocytophilum in Finnish dogs. (Thesis). University of Helsinki. Retrieved from http://hdl.handle.net/10138/165474
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Kapiainen, Suvi. “The prevalence of Anaplasma phagocytophilum in Finnish dogs.” 2016. Thesis, University of Helsinki. Accessed March 04, 2021.
http://hdl.handle.net/10138/165474.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Kapiainen, Suvi. “The prevalence of Anaplasma phagocytophilum in Finnish dogs.” 2016. Web. 04 Mar 2021.
Vancouver:
Kapiainen S. The prevalence of Anaplasma phagocytophilum in Finnish dogs. [Internet] [Thesis]. University of Helsinki; 2016. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/10138/165474.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Kapiainen S. The prevalence of Anaplasma phagocytophilum in Finnish dogs. [Thesis]. University of Helsinki; 2016. Available from: http://hdl.handle.net/10138/165474
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Helsinki
10.
Joenperä, Jasmin.
Borrelia burgdorferi- ja Anaplasma phagocytophilum –vasta-aineiden prevalentti suomalaisilla hevosilla.
Degree: Department of Veterinary Biosciences; Helsingin yliopisto, Eläinlääketieteellinen tiedekunta, Eläinlääketieteellisten biotieteiden osasto; Helsingfors universitet, Veterinärmedicinska fakulteten, Avdelningen för veterinärmedicinska biovetenskap, 2015, University of Helsinki
URL: http://hdl.handle.net/10138/156243
► Borrelia burgdorferi ja Anaplasma phagocytophilum ovat puutiaisvälitteisiä bakteereja. B. burgdorferi aiheuttaa ihmisille borrelioosia, mutta sen merkitystä hevosten taudinaiheuttajana ei täysin tunneta. A. phagocytophilum puolestaan aiheuttaa…
(more)
▼ Borrelia burgdorferi ja Anaplasma phagocytophilum ovat puutiaisvälitteisiä bakteereja. B. burgdorferi aiheuttaa ihmisille borrelioosia, mutta sen merkitystä hevosten taudinaiheuttajana ei täysin tunneta. A. phagocytophilum puolestaan aiheuttaa granulosyyttistä anaplasmoosia ihmisillä, koiralla ja hevosella sekä laidunkuumetta märehtijöillä. Tämän tutkimuksen tarkoituksena oli tutkia Borrelia- ja Anaplasma-vasta-aineiden yleisyyttä suomalaisilla hevosilla. Eri Euroopan maissa tehdyissä tutkimuksissa on todettu Borrelia-vasta-aineita noin 3-29 %:lla ja Anaplasma-vasta-aineita noin 9-73 %:lla hevosista. Suomessa aihetta ei ole aiemmin tutkittu tässä mittakaavassa. Hypoteesina oli, että hevosista löydetään vasta-aineita molempia taudinaiheuttajia vastaan erityisesti etelässä ja rannikkoalueilla sekä iäkkäillä ja Suomeen tuoduilla hevosilla.
Tutkimuksessa kerättiin 281 varsojen ja 319 aikuisten hevosten verinäytettä Manner-Suomesta ja Ahvenanmaalta. Hevosenomistajilta pyydettiin lisäksi tietoja hevosesta kysymyslomakkeella. Näytteet tutkittiin koirien vektorivälitteisten tartuntojen diagnostiikkaan kehitettyä SNAP 4DX Plus –pikatestiä. Tulosten tilastollinen analyysi suoritettiin SPSS-ohjelmalla käyttäen yhden muuttujan logistista regressioanalyysiä ja ristiintaulukointia.
Borrelia-vasta-aineita todettiin 60/319 (18,8 %) aikuisella ja 11/281 (3,9 %) varsalla ja Anaplasma-vasta-aineita 20/319 (6,3 %) aikuisella ja 4/281 varsalla (1,4 %). Seroprevalenssit olivat korkeimmat Ahvenanmaalla (aikuisilla Borrelia 89,5 % ja Anaplasma 47,4 %), Etelä-Suomessa (25,5 % ja 4.9 %) sekä Itä-Suomessa (17,0 % ja 4,9 %). Borrelia-seropositiivisuuden kannalta tilastollisesti merkitseviä tekijöitä olivat hevosessa havaitut puutiaiset, alue, hevosen alkuperämaa, ikä, käyttötarkoitus, roturyhmä sekä Anaplasma-seropositiivisuus yhden muuttujan mallissa. Anaplasma-seropositiivisuuden kannalta mahdollisesti merkitseviä tekijöitä olivat puutiaiset, alue, hevosen alkuperämaa, ikä sekä Borrelia-seropositiivisuus.
Tulosten perusteella Borrelia burgdorferi- ja Anaplasma phagocytophilum -tartuntoja esiintyy yleisesti suomalaisilla hevosilla. Tartuntapaine oli korkein Ahvenanmaalla sekä Etelä- ja Itä-Suomessa. Käytännössä bakteeria kantavan puutiaisen purema on ainoa syy tartuntaan, mutta taustatekijät kuten asuminen puutiaisen levinneisyysalueella tai ulkoileminen maastossa tai laitumella ja voivat altistaa hevosen puutiaisille ja siten Borrelia- tai Anaplasma-tartunnalle. Riskitekijöiden selvittäminen vaatii laajempaa tilastollista analyysiä ja esimerkiksi Borrelia-tartunnan mahdollista yhteyttä kliinisiin oireisiin pitäisi selvittää lisätutkimuksin.
Subjects/Keywords: granulosyyttinen anaplasmoosi; seroprevalenssi; Borrelia burgdorferi; Anaplasma phagocytophilum; borrelioosi; hevonen; vasta-aineet; Mikrobiologia ja epidemiologia; Immunologia; Mikrobiologi och epidemiologi; Immunologi; Microbiology and Epidemiology; Immunology; granulosyyttinen anaplasmoosi; seroprevalenssi; Borrelia burgdorferi; Anaplasma phagocytophilum
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Joenperä, J. (2015). Borrelia burgdorferi- ja Anaplasma phagocytophilum –vasta-aineiden prevalentti suomalaisilla hevosilla. (Thesis). University of Helsinki. Retrieved from http://hdl.handle.net/10138/156243
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Joenperä, Jasmin. “Borrelia burgdorferi- ja Anaplasma phagocytophilum –vasta-aineiden prevalentti suomalaisilla hevosilla.” 2015. Thesis, University of Helsinki. Accessed March 04, 2021.
http://hdl.handle.net/10138/156243.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Joenperä, Jasmin. “Borrelia burgdorferi- ja Anaplasma phagocytophilum –vasta-aineiden prevalentti suomalaisilla hevosilla.” 2015. Web. 04 Mar 2021.
Vancouver:
Joenperä J. Borrelia burgdorferi- ja Anaplasma phagocytophilum –vasta-aineiden prevalentti suomalaisilla hevosilla. [Internet] [Thesis]. University of Helsinki; 2015. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/10138/156243.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Joenperä J. Borrelia burgdorferi- ja Anaplasma phagocytophilum –vasta-aineiden prevalentti suomalaisilla hevosilla. [Thesis]. University of Helsinki; 2015. Available from: http://hdl.handle.net/10138/156243
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
11.
Ribeiro, Ana Jacinta Escrivães.
Seroprevalência de Anaplasma phagocytophilum em cavalos do norte de Portugal.
Degree: 2014, RCAAP
URL: http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/3101
► Dissertação de Mestrado Integrado em Medicina Veterinária, Ciências Veterinárias
A bactéria Anaplasma phagocytophilum (ordem Rickettsiales), gram-negativa e intracelular obrigatória, é agente da anaplasmose granulocítica (da…
(more)
▼ Dissertação de Mestrado Integrado em Medicina Veterinária, Ciências Veterinárias
A bactéria Anaplasma phagocytophilum (ordem Rickettsiales), gram-negativa e intracelular obrigatória, é agente da anaplasmose granulocítica (da reformulada erliquiose granulocítica) em várias espécies de animais domésticos e selvagens, nomeadamente os equinos, tendo carácter zoonótico. Este agente transmite-se através de um vector ixodídeo, concretamente carraças do género Ixodes. Os sinais clínicos incluem febre, depressão, anorexia, edema dos membros, ataxia e icterícia, observando-se também alterações hematológicas, como trombocitopenia, leucopenia e diminuição do hematócrito.
Pretendeu-se estudar a seroprevalência de anticorpos para a bactéria Anaplasma phagocytophilum em cavalos residentes na região Norte de Portugal, através do teste de imunofluorescência indirecta (IFI).
Foram efectuadas recolhas de sangue venoso central de 166 cavalos residentes há mais de um mês na região Norte. Para cada animal foi realizado exame físico e um inquérito que permitiu o registo de informação relativa ao género, idade, aptidão, regime de estabulação, distrito e presença de sinais clínicos. O soro obtido foi utilizado para a realização do teste IFI, disponível comercialmente (Anaplasma phagocytophila, IFA substrate slide, VMRD, Inc.). Os soros foram testados na diluição de 1:50. Demonstrou-se a presença de anticorpos contra A. phagocytophilum em 12,7% dos animais testados (21: 166).
Este estudo, apesar de não ter definido títulos de anticorpos (usando um “cut-off” ≥ 50), é revelador da existência de anticorpos contra A. phagocytophilum em cavalos na região Norte de Portugal. Trata-se de informação a registar no âmbito da actividade clínica em equinos e também a ter em conta para a realização de estudos futuros em doenças causadas por agentes transmitidos por carraças.
Anaplasma phagocytophilum (Rickettsiales order) is a gram negative and obligate intracellular bacteria, responsible for granulocytic anaplasmosis (former granulocytic ehrlichiosis) in various species of domestic and wild animals, including equines. This zoonotic agent spreads through an arthropod vector, concretely an Ixodes spp. tick. The clinical signs include fever, depression, anorexia, edema of the extremities, ataxia and icterus. Hematological abnormalities like thrombocytopenia, leucopenia and a decreased hematocrit can also occur.
Our aim was to study the prevalence of antibodies against Anaplasma phagocytophilum in the serum of horses via the indirect imunofluorescence assay.
Venous blood samples were taken from 166 horses residing in the Northern region of Portugal for more than one month. A physical exam and a questionnaire regarding gender, age, presence of clinical signs, ability, housing arrangement and district were performed for each animal. The serum was submitted to the indirect immunofluorescence assay commercially available (Anaplasma phagocytophila, IFA substract slide, VMRD, Inc). The serum was tested using a 1:50 dilution. The presence of antibodies against…
Advisors/Committee Members: Cotovio, Mário Pedro Gonçalves.
Subjects/Keywords: Anaplasma phagocytophilum; Prevalência; Região Norte (Portugal); Bactéria gram-negativa; Serologia; Cavalos; Teste de imunoflurescência indireta
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APA (6th Edition):
Ribeiro, A. J. E. (2014). Seroprevalência de Anaplasma phagocytophilum em cavalos do norte de Portugal. (Thesis). RCAAP. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/3101
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ribeiro, Ana Jacinta Escrivães. “Seroprevalência de Anaplasma phagocytophilum em cavalos do norte de Portugal.” 2014. Thesis, RCAAP. Accessed March 04, 2021.
http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/3101.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ribeiro, Ana Jacinta Escrivães. “Seroprevalência de Anaplasma phagocytophilum em cavalos do norte de Portugal.” 2014. Web. 04 Mar 2021.
Vancouver:
Ribeiro AJE. Seroprevalência de Anaplasma phagocytophilum em cavalos do norte de Portugal. [Internet] [Thesis]. RCAAP; 2014. [cited 2021 Mar 04].
Available from: http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/3101.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ribeiro AJE. Seroprevalência de Anaplasma phagocytophilum em cavalos do norte de Portugal. [Thesis]. RCAAP; 2014. Available from: http://www.rcaap.pt/detail.jsp?id=oai:http://repositorio.utad.pt/:10348/3101
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universiteit Utrecht
12.
Oldehinkel, A.
Moleculaire detectie van teken-gebonden zoönosen in Ixodes hexagonus teken afkomstig van katten in Nederland.
Degree: 2009, Universiteit Utrecht
URL: http://dspace.library.uu.nl:8080/handle/1874/40136
► Ticks are vectors of a great diversity of pathogens, which can be of both veterinary and human medical interest. Besides the emerging of the number…
(more)
▼ Ticks are vectors of a great diversity of pathogens, which can be of both veterinary
and human medical interest. Besides the emerging of the number of vector-borne
diseases in humans, the frequency of the number of vector-borne diseases in
companion animals in Europe is also rising. This study has tried to map out the
prevalence of a number of tick-borne zoonoses in I. hexagonus ticks originating
from cats in the Netherlands.
Since the way of life of I. hexagonus is totally different from that of I. ricinus and
because there has only been limited research to I. hexagonus, there has been
chosen to investigate I. hexagonus ticks.
With screening by polymerase chain reaction (PCR) and revere line blot (RLB)
hybridization, 182 ♀ I. hexagonus ticks – collected from July 2005 up to
September 2009 – from the collection of the Utrecht Centre for Tick-borne
Diseases (UCTD), were screened for the presence of
Anaplasma spp., Bartonella
spp., Borrelia burgdorferi s.l., Coxiella burnetii, Francisella tularensis and
Rickettsia spp.
The screened I. hexagonus ticks were infected with A.
phagocytophilum (2.75%)
and B. schoenbuchensis (2.20%). This is the first time that B. schoenbuchensis is
demonstrated in ticks originating from cats. B. schoenbuchensis is associated with
the zoonosis deer ked dermatitis, although the exact role in the etiology of this
disease remains to be elucidated.
Further, there were positive results for R. helvetica and there was one positive
result for R. massiliae. Because the 16S rRNA sequences of 2 other samples which were positive for Rickettsia spp. by RLB did not match the 16S rRNA sequence of any known Rickettsia spp., these results should be carefully interpreted.
It was remarkable that B. burgdorferi sensu lato was not found in any of the
screened ticks.
On account of – during this investigation – encountered problems with a newly
developed diagnostic RLB test for Francisella and Coxiella, there are no reliable
results concerning the prevalence of these pathogens. At the moment the UCTD is
working to prevent such problems in the future.
To conclude: more research at tick-borne zoonoses is desirable, because not from
all infectious agents it is known whether they are a zoonosis or not and it is not
always known what the role of ticks is in the transmission of these agents.
Advisors/Committee Members: Jongejan, F..
Subjects/Keywords: Diergeneeskunde; Teken; Ticks; Ixodes hexagonus; Cats; Coxiella burnetii; Borrelia burgdorferi; Francisella tularensis; Bartonella spp.; Rickettsia spp.; Anaplasma phagocytophilum
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APA (6th Edition):
Oldehinkel, A. (2009). Moleculaire detectie van teken-gebonden zoönosen in Ixodes hexagonus teken afkomstig van katten in Nederland. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/40136
Chicago Manual of Style (16th Edition):
Oldehinkel, A. “Moleculaire detectie van teken-gebonden zoönosen in Ixodes hexagonus teken afkomstig van katten in Nederland.” 2009. Doctoral Dissertation, Universiteit Utrecht. Accessed March 04, 2021.
http://dspace.library.uu.nl:8080/handle/1874/40136.
MLA Handbook (7th Edition):
Oldehinkel, A. “Moleculaire detectie van teken-gebonden zoönosen in Ixodes hexagonus teken afkomstig van katten in Nederland.” 2009. Web. 04 Mar 2021.
Vancouver:
Oldehinkel A. Moleculaire detectie van teken-gebonden zoönosen in Ixodes hexagonus teken afkomstig van katten in Nederland. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2009. [cited 2021 Mar 04].
Available from: http://dspace.library.uu.nl:8080/handle/1874/40136.
Council of Science Editors:
Oldehinkel A. Moleculaire detectie van teken-gebonden zoönosen in Ixodes hexagonus teken afkomstig van katten in Nederland. [Doctoral Dissertation]. Universiteit Utrecht; 2009. Available from: http://dspace.library.uu.nl:8080/handle/1874/40136
Humboldt State University
13.
Sholty, Kathleen E.
Strain distribution of Borrelia burgdorferi and Anaplasma phagocytophilum in sciurids and woodrats in northwestern California.
Degree: MS, Natural Resources: Wildlife, 2015, Humboldt State University
URL: http://hdl.handle.net/10211.3/150383
► Borrelia burgdorferi (Lyme disease) and Anaplasma phagocytophilum (granulocytic anaplasmosis) are two tick-borne pathogens in the western United States. The ecology of both pathogens varies among…
(more)
▼ Borrelia burgdorferi (Lyme disease) and
Anaplasma phagocytophilum (granulocytic anaplasmosis) are two tick-borne pathogens in the western United States. The ecology of both pathogens varies among areas in northwestern California due to multiple rodent hosts, diverse landscapes, and multiple genotypes within B. burgdorferi sensu lato and A.
phagocytophilum sensu lato. I documented prevalence of both pathogens in Del Norte, Humboldt, and Mendocino counties and identified associations among hosts and strain types. Rodents were live-trapped between October 2005 and October 2013. Ear-punch biopsies and whole blood samples were collected to determine the presence of B. burgdorferi sensu lato and A.
phagocytophilum sensu lato via polymerase chain reaction (PCR), and positive samples were sequenced to determine genotypes. The program SaTScan was used to evaluate spatial clustering of genotypes. Overall prevalence of B. burgdorferi sensu lato and A.
phagocytophilum sensu lato between 2005-2013 was 33.8% and 18.9%, respectively. The top models predicting genotypes of B. burgdorferi were ranked via a corrected Akaike???s Information Criterion (AICc) value and included the variables ???Host,??? ???Habitat,??? and the interaction term ???Host*Season,??? and the top model predicting genotypes of A.
phagocytophilum included the single variable ???Host.??? Dusky-footed woodrats (Neotoma fuscipes) had a higher prevalence of B. bissettii than B. burgdorferi sensu stricto and both N. fuscipes and sciurids had a higher prevalence of the DU1 strain than A.
phagocytophilum sensu stricto. SaTScan detected clusters for both DU1 and B. bissettii at Del Norte study area (P<0.001 and P=0.01, respectively). Another cluster was found for DU1 at North Humboldt study area (P=0.001). A greater proportion of captured individuals found within each cluster were N. fuscipes. The results suggest a relationship between strain type and rodent host. Because B. burgdorferi sensu stricto and A.
phagocytophilum sensu stricto are more likely to be infective to humans, these results may help identify risks for transmission among humans living and recreating in northwestern California.
Advisors/Committee Members: Brown, Richard.
Subjects/Keywords: Borrelia burgdorferi; Anaplasma phagocytophilum; Lyme disease; Granulocytic anaplasmosis; Woodrats; Sciurids; California; Disease ecology; Tick-borne disease
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APA (6th Edition):
Sholty, K. E. (2015). Strain distribution of Borrelia burgdorferi and Anaplasma phagocytophilum in sciurids and woodrats in northwestern California. (Masters Thesis). Humboldt State University. Retrieved from http://hdl.handle.net/10211.3/150383
Chicago Manual of Style (16th Edition):
Sholty, Kathleen E. “Strain distribution of Borrelia burgdorferi and Anaplasma phagocytophilum in sciurids and woodrats in northwestern California.” 2015. Masters Thesis, Humboldt State University. Accessed March 04, 2021.
http://hdl.handle.net/10211.3/150383.
MLA Handbook (7th Edition):
Sholty, Kathleen E. “Strain distribution of Borrelia burgdorferi and Anaplasma phagocytophilum in sciurids and woodrats in northwestern California.” 2015. Web. 04 Mar 2021.
Vancouver:
Sholty KE. Strain distribution of Borrelia burgdorferi and Anaplasma phagocytophilum in sciurids and woodrats in northwestern California. [Internet] [Masters thesis]. Humboldt State University; 2015. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/10211.3/150383.
Council of Science Editors:
Sholty KE. Strain distribution of Borrelia burgdorferi and Anaplasma phagocytophilum in sciurids and woodrats in northwestern California. [Masters Thesis]. Humboldt State University; 2015. Available from: http://hdl.handle.net/10211.3/150383
14.
Taank, Vikas Kumar.
Arthropod Organic Anion Transporting Polypeptides in Tick-Borne Bacterial and Viral Infections.
Degree: PhD, Biological Sciences, 2019, Old Dominion University
URL: 9781687938367
;
https://digitalcommons.odu.edu/gradschool_biomedicalsciences_etds/2
► Vector-borne diseases (VBDs) are human illness transmitted by an arthropod vector. World Health Organization (WHO) estimates that VBD has a huge impact worldwide that…
(more)
▼ Vector-borne diseases (VBDs) are human illness transmitted by an arthropod vector. World Health Organization (WHO) estimates that VBD has a huge impact worldwide that is responsible for affecting a billion people and causes 700,000 deaths annually. In recent years, there has been a continuous increase in the incidences of tick-borne diseases such as Lyme diseases and human anaplasmosis as reported by Center for Disease Control and Prevention. Very few reliable VBD control strategies have emerged till now. Transmission-blocking vaccines can provide effective management of VBDs but requires identification and characterization of novel vector-pathogen conserved molecules that play a significant role in pathogen survival and transmission from the vector host. This work describes two studies identifying the role of conserved tick organic anion transporting polypeptides (OATPs) in bacterial and viral infections. In the first study, the results show that
A. phagocytophilum modulates a specific tick host organic anion transporting polypeptide (isoatp4056) and kynurenine aminotransferase (
kat), a gene responsible for the production of metabolite xanthurenic acid (XA) from tryptophan catabolism pathway, for its survival in I
xodes scapularis ticks. Silencing of
isoatp4056 expression using RNA interference revealed that this gene has no effect on bacterial acquisition from the murine host, but affects bacterial survival in tick cells. Furthermore, silencing of gene expression for either kat alone or with
isoatp4056 affected both bacterial survival and expression of
isoatp4056. Exogenous addition of XA revealed increased
isoatp4056 expression and bacterial burden in tick salivary gland and ticks cells. By using electrophoretic mobility shift assays (EMSA), the study provides evidence that both XA and
A. phagocytophilum influences regulation of
isoatp4056 gene.
The second part of the study focuses on characterization of the role of these conserved organic anion transporting polypeptides in association with Lyme disease agent
Borrelia burgdorferi and tick-borne Langat virus (LGTV), a viral pathogen closely related to tick-borne encephalitis virus (TBEV). Quantitative Real-Time PCR (QRT-PCR), data show that
B. burgdorferi has no impact on the arthropod oatps gene expression in unfed nymphal ticks. Similarly, synchronous LGTV infection of unfed ticks (nymphs) revealed no impact on the expression of tick OATPs. However, specific OATPs were significantly downregulated upon LGTV infection in ticks cells at 24 h but not at 72 h post infection (p.i.). Furthermore, OATP inhibitor (SPZ) treatment followed by LGTV infection of tick cells showed significant reduction of LGTV loads, expression of
kat gene, and several OATP genes. Bioinformatic characterization of medically important arthropod vectors including ticks, mosquitos, and lice revealed presence of several post-translational modifications sites such as glycosylation,…
Advisors/Committee Members: Girish Neelakanta, Hameeda Sultana, Piotr Kraj, Deborah Waller.
Subjects/Keywords: Anaplasma phagocytophilum; Borrelia burgdorferri; Langat virus; Organic anion transporting polypeptide; RNA interference; Xanthurenic acid; Biology; Microbiology; Molecular Biology
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APA ·
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APA (6th Edition):
Taank, V. K. (2019). Arthropod Organic Anion Transporting Polypeptides in Tick-Borne Bacterial and Viral Infections. (Doctoral Dissertation). Old Dominion University. Retrieved from 9781687938367 ; https://digitalcommons.odu.edu/gradschool_biomedicalsciences_etds/2
Chicago Manual of Style (16th Edition):
Taank, Vikas Kumar. “Arthropod Organic Anion Transporting Polypeptides in Tick-Borne Bacterial and Viral Infections.” 2019. Doctoral Dissertation, Old Dominion University. Accessed March 04, 2021.
9781687938367 ; https://digitalcommons.odu.edu/gradschool_biomedicalsciences_etds/2.
MLA Handbook (7th Edition):
Taank, Vikas Kumar. “Arthropod Organic Anion Transporting Polypeptides in Tick-Borne Bacterial and Viral Infections.” 2019. Web. 04 Mar 2021.
Vancouver:
Taank VK. Arthropod Organic Anion Transporting Polypeptides in Tick-Borne Bacterial and Viral Infections. [Internet] [Doctoral dissertation]. Old Dominion University; 2019. [cited 2021 Mar 04].
Available from: 9781687938367 ; https://digitalcommons.odu.edu/gradschool_biomedicalsciences_etds/2.
Council of Science Editors:
Taank VK. Arthropod Organic Anion Transporting Polypeptides in Tick-Borne Bacterial and Viral Infections. [Doctoral Dissertation]. Old Dominion University; 2019. Available from: 9781687938367 ; https://digitalcommons.odu.edu/gradschool_biomedicalsciences_etds/2
Vilnius University
15.
Steponkienė,
Ana.
Erkių platinamų ligų sukėlėjų paplitimas ir
molekulinė diagnostika.
Degree: Master, 2014, Vilnius University
URL: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2009~D_20140625_172954-48366
;
► Ixodes genties erkės yra krauju mintantys nariuotakojai, visų – stuburinių gyvūnų, taip pat žmogaus parazitai. Ixodes ricinus erkės perneša daugybę ligų sukėlėjų, tokių kaip: erkinio…
(more)
▼ Ixodes genties erkės yra krauju mintantys
nariuotakojai, visų – stuburinių gyvūnų, taip pat žmogaus
parazitai. Ixodes ricinus erkės perneša daugybę ligų sukėlėjų,
tokių kaip: erkinio encefalito (erkinio encefalito virusas), Laimo
ligos (Borrelia burgdorferi sensu lato bakterijos), bebeziozės
sukėlėjus (Babesia microti ir Babesia divergens). Taip pat platina
ir Anaplasma phagocytophilum bakterijas. Šiuo metu erkių platinamos
ligos yra dažniausios vektorių sukeliamos infekcijos Europoje.
Pastaraisiais metais išpopuliarėjo molekulinės biologijos metodai,
paremti patogenų nukleino rūgščių polimerazės grandinine reakcija
ir RLB hibridizacija. Šie metodai laikomi tinkamiausiais,
jautriausiais ir specifiškiausiais erkių platinamų ligų sukėlėjų
diagnostikai. Šiame darbe naudoti molekuliniai metodai erkių
platinamų ligų sukėlėjų: erkinio encefalito viruso, Anaplasma
phagocytophilum, Borrelia burgdorferi sensu lato ir Babesia spp.,
nustatymui iš 2200 erkių, surinktų keturiuose Lietuvos rajonuose.
Tyrimų metu nustatyti šeši erkinio encefalito viruso infekcijų
atvejai, iš kurių penki teigiami mėginiai nustatyti Radviliškio
raj. surinktose erkėse ir vienas – Utenos raj. Anaplasma
phagocytophilum bakterijų infekcijos yra dažniausiai pasitaikančios
infekcijos Lietuvos erkėse. Tyrimų metu nustatyti 37 (3 %) teigiami
Anaplasma phagocytophilum mėginiai, iš kurių 17 (46 %) nustatyti
Kėdainių raj. surinktose erkėse, 15 (32 %) – Klaipėdos, 5 (14 %) –
Radviliškio ir 3 (8 %) – Utenos raj. Tarp šių... [toliau žr. visą
tekstą]
Ticks of genus Ixodes that infect livestock,
deer, dogs, and a wide variety of other species including humans.
Ixodes ricinus can also transmit numerous diseases including
tick-borne encephalitis, Lyme diseases (Borrelia burgdorferi sensu
lato infections), babesiosis (Babesia microti and Babesia divergens
infections). It can also spread Anaplasma phagocytophilum bacteria.
Now tick-borne infections are the most frequent human vector-borne
infections in Europe, the incidence of these infections has been on
rise, and new infections have emerged. In recent years, molecular
detection methods based on PCR amplification of the nucleic acids
of pathogens and Reverse Line blot hybridization have been showed
to be effective, sensitive and specific methods for diagnosis of
tick-borne diseases. In this work were used molecular detection
methods for tick-borne pathogens: tick-borne encephalitis virus
(TBEV), Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato,
and Babesia spp., diagnostics in 2200 ticks collected in four
Lithuanian regions forests. During investigation were identified
six cases of TBEV, which five positive samples were from ticks
collected in Radviliškis region and one from Utenos region forest.
Anaplasma phagocytophilum bacteria infections were most frequent in
Lithuanian ticks, during researches were identified 37 (3 %) cases
(17 (46 %) in Kėdainių region, 15 (32 %) – Klaipėdos, 5 (14 %) –
Radviliškio, and 3 (8 %) – Utenos region). In these 81 % were in
adult... [to full text]
Advisors/Committee Members: Žygutienė, Milda (Master's thesis supervisor).
Subjects/Keywords: Erkių platinamų ligų
sukėlėjai; Erkinio encefalito
virusas; Anaplasma
phagocytophilum; Borrelia burgdorferi sensu
lato; Babesia spp; Molekulinė
diagnostika
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APA ·
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MLA ·
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CSE |
Export
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Manager
APA (6th Edition):
Steponkienė,
Ana. (2014). Erkių platinamų ligų sukėlėjų paplitimas ir
molekulinė diagnostika. (Masters Thesis). Vilnius University. Retrieved from http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2009~D_20140625_172954-48366 ;
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Chicago Manual of Style (16th Edition):
Steponkienė,
Ana. “Erkių platinamų ligų sukėlėjų paplitimas ir
molekulinė diagnostika.” 2014. Masters Thesis, Vilnius University. Accessed March 04, 2021.
http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2009~D_20140625_172954-48366 ;.
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
MLA Handbook (7th Edition):
Steponkienė,
Ana. “Erkių platinamų ligų sukėlėjų paplitimas ir
molekulinė diagnostika.” 2014. Web. 04 Mar 2021.
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Vancouver:
Steponkienė,
Ana. Erkių platinamų ligų sukėlėjų paplitimas ir
molekulinė diagnostika. [Internet] [Masters thesis]. Vilnius University; 2014. [cited 2021 Mar 04].
Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2009~D_20140625_172954-48366 ;.
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Council of Science Editors:
Steponkienė,
Ana. Erkių platinamų ligų sukėlėjų paplitimas ir
molekulinė diagnostika. [Masters Thesis]. Vilnius University; 2014. Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2009~D_20140625_172954-48366 ;
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Freie Universität Berlin
16.
Hagedorn, Peter.
Analysis of Ixodes, Haemaphysalis and Dermacentor ticks for tick-borne
pathogens around Berlin.
Degree: 2013, Freie Universität Berlin
URL: http://dx.doi.org/10.17169/refubium-8980
► Tick-borne pathogens are responsible for several human and livestock diseases with occasionally severe consequences. Therefore, it is relevant for the public health system of a…
(more)
▼ Tick-borne pathogens are responsible for several human and livestock diseases
with occasionally severe consequences. Therefore, it is relevant for the
public health system of a country to analyse the potential risk for tick-borne
infections to increase the awareness and introduce control measures. In
Germany surveillance of tick-borne diseases is usually limited to tick-borne
encephalitis (TBE) virus, while other important pathogens are omitted. Patchy
information on TBE incidence and tick distribution in the country is
available, with most of the studies focused on areas in the Southern and
Western part of Germany. Likewise, little is known about the distribution of
tick species and the harboured pathogens in the area of Berlin. In this study
we collected larvae, nymphs and adult ticks in seven representative sampling
sites around Berlin in a monthly planned routine over 28 months. Only month
with snow cover or sub-zero temperatures when no tick activity was expected
were excluded. Based on the total number of collected ticks the different
boroughs where ticks were collected were attributed to classes of tick
densities followed by the additional analysis of the presence of different
tick-borne pathogens (Borrelia burgdorferi sensu lato, Rickettsia spp.,
Babesia spp., and
Anaplasma phagocytophilum) in the collected ticks, using new
commercially available assays for this purpose. The results of these
investigations were validated with investigations done with conventional
molecularbiological methods. The results presented provide valuable data for
the estimation of the presence of ticks and the risk of infection by tick-
borne pathogens in the Berlin area. By broadening the collection to the larval
stage, it was possible to find a possible explanation why Berlin does not
belong to TBE risk areas. The detection of borrelia pathogenic for humans in
D. reticulatus supports the idea that D. reticulatus could be a source of
borrelia infections in humans. The detection of a lot of rickettsia species
illustrates that diseases caused by rickettsia should be taken into account
and physicians should display increased alertness to these diseases. To our
knowledge this is the first time Rickettsia raoultii has been revealed in I.
ricinus in Germany. The confirmation of Babesia and
Anaplasma phagocytophilum
in ticks collected around Berlin demonstrates that the appearance of
babesiosis and human granulocytic anaplasmosis in this area has to be also
considered. In the area of Brieskow-Finkenheerd Haemaphysalis concinna
appeared as an established population with a high number of ticks of all
stages. To identify the possible transmission of human pathogens by
Haemaphysalis concinna further investigations are needed.
Advisors/Committee Members: [email protected] (contact), m (gender), Prof. Dr. Matthias Niedrig (RKI/HUB) (firstReferee), Prof. Dr. Rupert Mutzel (FUB) (furtherReferee).
Subjects/Keywords: Tick-borne pathogens; Ixodes ricinus; Dermacentor reticulatus; Borrelia burgdorferi; Babesia spp.; Anaplasma phagocytophilum;
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APA (6th Edition):
Hagedorn, P. (2013). Analysis of Ixodes, Haemaphysalis and Dermacentor ticks for tick-borne
pathogens around Berlin. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-8980
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Hagedorn, Peter. “Analysis of Ixodes, Haemaphysalis and Dermacentor ticks for tick-borne
pathogens around Berlin.” 2013. Thesis, Freie Universität Berlin. Accessed March 04, 2021.
http://dx.doi.org/10.17169/refubium-8980.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Hagedorn, Peter. “Analysis of Ixodes, Haemaphysalis and Dermacentor ticks for tick-borne
pathogens around Berlin.” 2013. Web. 04 Mar 2021.
Vancouver:
Hagedorn P. Analysis of Ixodes, Haemaphysalis and Dermacentor ticks for tick-borne
pathogens around Berlin. [Internet] [Thesis]. Freie Universität Berlin; 2013. [cited 2021 Mar 04].
Available from: http://dx.doi.org/10.17169/refubium-8980.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Hagedorn P. Analysis of Ixodes, Haemaphysalis and Dermacentor ticks for tick-borne
pathogens around Berlin. [Thesis]. Freie Universität Berlin; 2013. Available from: http://dx.doi.org/10.17169/refubium-8980
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Linköping University
17.
Tompa, Eszter.
Detektion av Anaplasma phagocytophilum och Rickettsia spp. hos Ixodes persulcatus i Norrbotten, Sverige med realtids-PCR.
Degree: Clinical and Experimental Medicine, 2019, Linköping University
URL: http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-162980
► Detta är den första studien där förekomsten av Anaplasma phagocytophilum och Rickettsia spp. hos Ixodes persulcatus fästingar insamlade från Sverige undersökts. Syftet med denna…
(more)
▼ Detta är den första studien där förekomsten av Anaplasma phagocytophilum och Rickettsia spp. hos Ixodes persulcatus fästingar insamlade från Sverige undersökts. Syftet med denna studie var att undersöka förekomsten av Anaplasma phagocytophilum och Rickettsia spp. hos I. persulcatus i Norrbotten, Sverige. Fästingar livnär sig på blod och är vektor för flera olika patogena mikroorganismer. I Sverige är den vanligaste arten Ixodes ricinus men 2016 fann forskare etablerade populationer även av I. persulcatus i norra Sverige. I. persulcatus har bl.a. människa som värddjur och är vektor för bl.a. Anaplasma phagocytophilum och Rickettsia spp. Totalt analyserades 292 I. persulcatus fästingar med realtids-PCR med Anaplasma och Rickettsia gltA som målgen. Positiva prover analyserades med konventionell PCR med 17-kDa och ompB som målgener. PCR produkter nukleotidsekvenserades för artbestämning. Resultaten är att prevalensen av A. phagocytophilum är 0 % och prevalensen av Rickettsia spp. är 0.68 % (n=2) vilket är betydligt lägre än i andra länder. Av de två positiva proverna kunde en artbestämmas till R. helvetica och den andra kunde inte artbestämmas. De 292 fästingarna har tidigare analyserats för andra patogena mikroorganismer. I jämförelse med andra länder är saminfektionen lägre för dessa fästingar. Kunskap om I. persulcatus utbredningsområde i Sverige och vilka patogena mikroorganismer de är vektor för är av värde för flera olika grupper i samhället, möjligtvis framför allt för vårdpersonal, djurägare och personer som vistas mycket i riskområden.
Subjects/Keywords: Anaplasma phagocytophilum; Rickettsia; Ixodes persulcatus; Sverige; realtids-PCR; Microbiology in the medical area; Mikrobiologi inom det medicinska området
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APA (6th Edition):
Tompa, E. (2019). Detektion av Anaplasma phagocytophilum och Rickettsia spp. hos Ixodes persulcatus i Norrbotten, Sverige med realtids-PCR. (Thesis). Linköping University. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-162980
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Tompa, Eszter. “Detektion av Anaplasma phagocytophilum och Rickettsia spp. hos Ixodes persulcatus i Norrbotten, Sverige med realtids-PCR.” 2019. Thesis, Linköping University. Accessed March 04, 2021.
http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-162980.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Tompa, Eszter. “Detektion av Anaplasma phagocytophilum och Rickettsia spp. hos Ixodes persulcatus i Norrbotten, Sverige med realtids-PCR.” 2019. Web. 04 Mar 2021.
Vancouver:
Tompa E. Detektion av Anaplasma phagocytophilum och Rickettsia spp. hos Ixodes persulcatus i Norrbotten, Sverige med realtids-PCR. [Internet] [Thesis]. Linköping University; 2019. [cited 2021 Mar 04].
Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-162980.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Tompa E. Detektion av Anaplasma phagocytophilum och Rickettsia spp. hos Ixodes persulcatus i Norrbotten, Sverige med realtids-PCR. [Thesis]. Linköping University; 2019. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-162980
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universiteit Utrecht
18.
Cuperus, T.
The pathogenesis of Anaplasma phagocytophilum infections.
Degree: 2010, Universiteit Utrecht
URL: http://dspace.library.uu.nl:8080/handle/1874/42361
► Anaplasma phagocytophilum is a tick-borne, granulocytotropic bacterium causing a nonspecific febrile illness in humans and multiple domestic animals including cattle, sheep, horses and dogs. Since…
(more)
▼ Anaplasma phagocytophilum is a tick-borne, granulocytotropic bacterium causing a nonspecific febrile illness in humans and multiple domestic animals including cattle, sheep, horses and dogs. Since the discovery of A.
phagocytophilum as a human pathogen approximately 15 years ago, a large effort has been made to investigate the pathogenesis of this intriguing bacterium. Making use of different leukemic cell lines and knock-out mouse models many cellular and molecular pathogenic mechanisms have been unearthed. The picture arising from this research is one of a bacterium employing unique mechanisms to survive in its dangerous neutrophilic host cell. By entering via receptor-mediated endocytosis and residing in a vacuole which does not fuse with lysosomes, A.
phagocytophilum evades the default pathway of bacterial phagocytosis and lysosomal destruction. Subsequently, the bacterium downregulates microbicidal functions of the neutrophil, while at the same time prolonging its life span and using some activated functions for its own benefit. This leaves the infected host with dysregulated neutrophils, on the one hand unable to act as microbial killers, while on the other hand participating in proinflammatory reactions. Also beyond the neutrophil, infection with A.
phagocytophilum causes disorder. Both hematological pathology and inflammatory tissue lesions seem to be caused by a poorly regulated inflammatory process. Bacterial burden does not directly correlate with severity of pathology, therefore the question arises to what degree disease in A.
phagocytophilum infection is caused by direct bacterial mechanisms or the host’s own immune responses. Most likely, cytokine secretion of infected neutrophils plays an initiatory role in pathology, with subsequent steps in pathogenesis being caused by the dysregulated immune system of the host.
Advisors/Committee Members: Gröne, Prof. Dr. A..
Subjects/Keywords: Geneeskunde; anaplasma phagocytophilum, tick-borne disease, granulocytic anaplasmosis, infection, pathogenesis, HGA, TBF, tick borne fever, EGA, CGA, zoonosis, innate immune system, neutrophil
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APA ·
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APA (6th Edition):
Cuperus, T. (2010). The pathogenesis of Anaplasma phagocytophilum infections. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/42361
Chicago Manual of Style (16th Edition):
Cuperus, T. “The pathogenesis of Anaplasma phagocytophilum infections.” 2010. Masters Thesis, Universiteit Utrecht. Accessed March 04, 2021.
http://dspace.library.uu.nl:8080/handle/1874/42361.
MLA Handbook (7th Edition):
Cuperus, T. “The pathogenesis of Anaplasma phagocytophilum infections.” 2010. Web. 04 Mar 2021.
Vancouver:
Cuperus T. The pathogenesis of Anaplasma phagocytophilum infections. [Internet] [Masters thesis]. Universiteit Utrecht; 2010. [cited 2021 Mar 04].
Available from: http://dspace.library.uu.nl:8080/handle/1874/42361.
Council of Science Editors:
Cuperus T. The pathogenesis of Anaplasma phagocytophilum infections. [Masters Thesis]. Universiteit Utrecht; 2010. Available from: http://dspace.library.uu.nl:8080/handle/1874/42361
19.
Katsogiannou, Eleni.
Επίδραση φυσιολογικών και παθολογικών παραγόντων σε αιματολογικές παραμέτρους των μηρυκαστικών.
Degree: 2020, University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας
URL: http://hdl.handle.net/10442/hedi/47772
► This thesis aimed to assess the effect of physiological factors that act before or during the complete blood count analysis, as well as pathological factors…
(more)
▼ This thesis aimed to assess the effect of physiological factors that act before or during the complete blood count analysis, as well as pathological factors which can affect the hematological variables of ruminants. Among these factors, three physiological, two pre-analyticals (age, B12-rumen function) and one analytical (method), as well as one pathological factor (Anaplasma phagocytophilum), were selected and assessed. In Chapter I, the literature regarding the factors having an impact on the values of the hematological variables of ruminants are thoroughly reviewed. More specifically, factors acting at the pre-analytical, analytical or post-analytical phase and could alter the results of hematological variables are reported. In addition, the main pathological factors that affect blood cell counts and morphology of ruminants and the changes that are observed are presented. Their classification is based on the type of cells that undergo the most significant changes. In Chapter II, B12 synthesis, metabolism, function, requirements and the effect of B12 deficiency or toxicosis on ruminant health, are briefly reported. The study presented in this chapter regards the comparison of the concentrations of vitamin B12 and the values of hematological variables before and after weaning in veal calves and the investigation of the correlation between the concentration of vitamin B12 in blood and the hematological variables in calves before and after weaning. To this aim, blood was collected form 31 pre-weaned and 31 weaned calves of Limousine breed from the same farm. Complete blood count was performed in automated hematological analyzer and by blood smear evaluation and serum B12, total protein and albumin concentrations were measured. The serum concentration of vitamin B12, the HCT, the HGB, the platelet count and the lymphocyte count were significantly higher in weaned compared to pre-weaned calves. A very strong positive correlation was found between the concentration of vitamin B12 and HCT and HGB before weaning while these correlations were moderate positive after weaning and in the total population tested as well. In Chapter III, methods for the determination of the complete blood cell count in automatic hematological analyzers are described. Subsequently, the comparative study on WBC and platelet count determination is reported. The aim of this study was the comparison of the results of the WBC count evaluated microscopically in blood smears with those obtained by ADVIA 120 hematology analyzer and the validation of the appropriate correction factors for the manual technique, as well as the assessment of two suggested factors for platelet calculation in blood smears in sheep. In order to generate the regression equation between the average WBC count per field and the WBC count determined by ADVIA 120, 57 ovine blood samples were used. Then, 31 new ovine samples were used for the assessment of the agreement between the calculated WBC count based on the generated equation and those obtained by the analyzer. Similarly,…
Subjects/Keywords: Φυσιολογικοί παράγοντες; Παθολογικοί παράγοντες; Αιματολογικοί παράμετροι; Μηρυκαστικά; Βιταμίνη Β12; Μέθοδος μικροσκοπικής εξέτασης αίματος; Physiological factor; Pathological factor; Hematological variables; Ruminants; Vitamin B12; Blood smear microscopy; Anaplasma phagocytophilum
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APA ·
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APA (6th Edition):
Katsogiannou, E. (2020). Επίδραση φυσιολογικών και παθολογικών παραγόντων σε αιματολογικές παραμέτρους των μηρυκαστικών. (Thesis). University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας. Retrieved from http://hdl.handle.net/10442/hedi/47772
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Katsogiannou, Eleni. “Επίδραση φυσιολογικών και παθολογικών παραγόντων σε αιματολογικές παραμέτρους των μηρυκαστικών.” 2020. Thesis, University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας. Accessed March 04, 2021.
http://hdl.handle.net/10442/hedi/47772.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Katsogiannou, Eleni. “Επίδραση φυσιολογικών και παθολογικών παραγόντων σε αιματολογικές παραμέτρους των μηρυκαστικών.” 2020. Web. 04 Mar 2021.
Vancouver:
Katsogiannou E. Επίδραση φυσιολογικών και παθολογικών παραγόντων σε αιματολογικές παραμέτρους των μηρυκαστικών. [Internet] [Thesis]. University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας; 2020. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/10442/hedi/47772.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Katsogiannou E. Επίδραση φυσιολογικών και παθολογικών παραγόντων σε αιματολογικές παραμέτρους των μηρυκαστικών. [Thesis]. University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας; 2020. Available from: http://hdl.handle.net/10442/hedi/47772
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
20.
A. DELLA PEPA.
VECTOR-BORNE DISEASES IN COLONY STRAY CATS OF MILAN CITY.
Degree: 2013, Università degli Studi di Milano
URL: http://hdl.handle.net/2434/219128
► LEISHMANIA INFANTUM INFECTION IN STRAY CATS IN A NON-ENDEMIC AREA IN NORTHERN ITALY E. Spada, DVM, PhD, Researcher 1, A. Della Pepa, DVM 1, A.…
(more)
▼ LEISHMANIA INFANTUM INFECTION IN STRAY CATS IN A NON-ENDEMIC AREA IN NORTHERN ITALY
E. Spada, DVM, PhD, Researcher 1, A. Della Pepa, DVM 1,
A. Migliazzo, DVM, PhD 2, G. Bagnagatti De Giorgi, DVM 1,
R. Perego, DVM, PhD 1, D. Proverbio, DVM, PhD, Professor 1
1Dipartimento di Scienze Veterinarie per la Salute, la Produzione Animale e
la Sicurezza Alimentare, Universita degli Studi di Milano, Milan, Italy
2Centro di Referenza Nazionale per le Leishmaniosi,Istituto Zooprofilattico
Sperimentale della Sicilia, Palermo, Italy
Tipologia: Ricerca Originale
Area di interesse: Medicina interna
Purpose of the work.
To assess the prevalence of leishmaniosis in a large representative sample of stray cats from this non-endemic area, and to analyze the results according to clinical, laboratory and infectious data
2. MOLECULAR STUDY ON VECTOR-BORNE INFECTIONS IN URBAN STRAY COLONY CAT IN NORTHENRN ITALY
Eva Spada§, DVM, PhD, Researcher
Daniela Proverbio§, DVM, PhD, Professor
Alessandra Della Pepa§, DVM
Paola Galluzzo*, Biologist
Roberta Perego§, DVM, PhD
Giada Bagnagatti De Giorgi§, DVM
Abstract
Feline vector-borne diseases are caused by a wide range of pathogens, which are transmitted by arthropods. Many of these infections have zoonotic implications and feral cats may potentially act as sentinels of human and pet health. The present study investigated the prevalence of vector-borne infections in feral colony cats in the city of Milan in northern Italy. Blood samples from 260 feral cats were evaluated, with conventional PCR, for the presence of DNA associated with hemoplasmas (Mycoplasma haemofelis and Mycoplasma haemominutum), Rickettsia spp.,
Anaplasma phagocytophilum, Ehrlichia spp. and Babesia microti. Odd ratios (OR) were calculated to identify risk factors for infection with vector-borne pathogens. Positive PCR was found in 156 out of 260 subjects (60%), with a prevalence of 33.1% for hemoplasmas, 31.9% for Rickettsia spp., 17.7% for A.
phagocytophilum , 6.7% for Ehrlichia spp. (out of 30 samples), and 1.2% for B. microti spp (out of 168 samples). Statistical analysis revealed a correlation between infections with Rickettsia spp. and hemoplasmas (OR=1.95, P=0.02). Additionally, Rickettsia spp. infection was associated with ocular infection (OR=2.21, P=0.02). We conclude that vector-borne infections, including zoonotic diseases, are present in feral cats of Milan. Thus, domestic cats exposed to the outdoors should be routinely monitored and treated for ectoparasites to minimize disease onset and potential transmission of zoonotic agents to humans. Moreover, as these vector-borne infections are transmitted through blood, feline blood donors from this area should be screened by PCR.
Advisors/Committee Members: tutor: D. Proverbio, correlator: E. Spada, coordinator: F. Cremonesi, PROVERBIO, DANIELA, CREMONESI, FAUSTO.
Subjects/Keywords: feral cats; northern Italy; PCR; vector-borne infection; Mycoplasma haemofelis; Mycoplasma haemominutum; haemoplasmas; Rickettsia spp.; Anaplasma phagocytophilum; Ehrlichia spp.; Babesia microti; Leishmania infantum; Settore MED/09 - Medicina Interna
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APA (6th Edition):
PEPA, A. D. (2013). VECTOR-BORNE DISEASES IN COLONY STRAY CATS OF MILAN CITY. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/219128
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
PEPA, A. DELLA. “VECTOR-BORNE DISEASES IN COLONY STRAY CATS OF MILAN CITY.” 2013. Thesis, Università degli Studi di Milano. Accessed March 04, 2021.
http://hdl.handle.net/2434/219128.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
PEPA, A. DELLA. “VECTOR-BORNE DISEASES IN COLONY STRAY CATS OF MILAN CITY.” 2013. Web. 04 Mar 2021.
Vancouver:
PEPA AD. VECTOR-BORNE DISEASES IN COLONY STRAY CATS OF MILAN CITY. [Internet] [Thesis]. Università degli Studi di Milano; 2013. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/2434/219128.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
PEPA AD. VECTOR-BORNE DISEASES IN COLONY STRAY CATS OF MILAN CITY. [Thesis]. Università degli Studi di Milano; 2013. Available from: http://hdl.handle.net/2434/219128
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Freie Universität Berlin
21.
Schreiber, Cécile.
Prevalence and risk of infection.
Degree: 2014, Freie Universität Berlin
URL: http://dx.doi.org/10.17169/refubium-6749
► Vectorborne diseases are an important part of the clinical veterinary medicine of dogs. For these diseases ticks play a major role as vectors of different…
(more)
▼ Vectorborne diseases are an important part of the clinical veterinary medicine
of dogs. For these diseases ticks play a major role as vectors of different
infectious agents. The spreading of ticks is enhanced by an increased
transport of animals cross country borders, the absence of cold and long
winters as well as the re-naturation of agricultural areas. Today, the
emergence of infectious diseases as well as the appearance of coinfections
pose a challenge during the clinical work of veterinarians. To gather
information about the current risk for dogs regarding tick borne diseases this
study focused on the detection of four groups of pathogens: Babesia spp,
Borrelia spp., Rickettsia spp. and Anaplasmataceae. All examined ticks were
collected from dogs living in Berlin and Brandenburg. As part of the risk
analysis, scutal indices (SI) were determined for each tick in order to obtain
a rough estimation of duration of feeding. All analysed ticks were collected
between March 2010 and April 2011 in the course of a previous study regarding
tick control and infestation patterns of dogs at the Small Animal Clinic of
the Freie Universität Berlin (Beck, 2012) (Klinik und Poliklinik für kleine
Haustiere der Freien Universität Berlin). During the study participants
collected I. ricinus, I. hexagonus and D. reticulatus ticks from their dogs.
These ticks were used for SI measurements (1693 ticks) and the detection of
infectious agents (1255 ticks) by means of PCR, sequencing, or High Resolution
Melting Curve Analysis (HRM). According to SI measurements, over 40% of
examined female I. ricinus (n=480) and over 60% (n=647) of examined female D.
reticulatus were removed after a feeding time of at least two days. Female
ticks had a significantly higher SI than males (p<0.001). Furthermore, a
significant correlation between the fur length of the dogs and the SI of the
tick species D. reticulatus was discovered (p<0.05). SI of ticks and the
probability of an infection correlated positively (p<2.2#10-7). Prevalences of
Babesia spp. in I. ricinus and I. hexagonus were found to be as high as 2.5%
(CI: 0.92-5.45%) and 3% (CI: 0.77-7.95%) respectively, sequencing revealed B.
microti, B. capreoli and B. venatorum. Rickettsia spp. infected 61% (CI:
54.2-67.5%) of I. ricinus and 39% (CI: 33.7-45.2%) of D. reticulatus as well
as 44% (CI: 36.6-52.4%) of I. hexagonus. Detected genospecies were R.
helvetica, R. raoulti, and R. monacensis. Of all examined I. ricinus ticks,
6.5% (CI: 4.9-8.4%) and 4.3% (CI: 3.0-5.9%) were infected by A.
phagocytophilum and Candidatus Neoehrlichia mikurensis, respectively. Examined
I. hexagonus had a prevalence of 3.9% (CI: 1.6-8.0%) for A.
phagocytophilum
and 5.9% (CI: 2.9-10.6%) for Candidatus Neoehrlichia mikurensis. Prevalences
for Borrelia spp. were found to be as high as 11.6% (CI: 9.5-14.0%) in I.
ricinus and 11.2% (CI: 6.9-17.0%). In I. hexagonus. Borrelia spp. were
identified as B. afzelii, B. garinii, B. burgdorferi sensu strict and B.
miyamotoi. Coinfections were found in 20% (CI: 14.5-26.5%) of I. ricinus…
Advisors/Committee Members: w (gender), Univ.-Prof. Dr. Barbara Kohn (firstReferee), Univ.-Prof. Dr. Georg von Samson-Himmelstjerna (furtherReferee), Univ.-Prof. Dr. Lothar Wieler (furtherReferee).
Subjects/Keywords: ticks; tickborne diseases; infectious diseases; risk analysis; Borrelia; Anaplasma phagocytophilum; Neoehrlichia mikurensis; Ric; 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Schreiber, C. (2014). Prevalence and risk of infection. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-6749
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Schreiber, Cécile. “Prevalence and risk of infection.” 2014. Thesis, Freie Universität Berlin. Accessed March 04, 2021.
http://dx.doi.org/10.17169/refubium-6749.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Schreiber, Cécile. “Prevalence and risk of infection.” 2014. Web. 04 Mar 2021.
Vancouver:
Schreiber C. Prevalence and risk of infection. [Internet] [Thesis]. Freie Universität Berlin; 2014. [cited 2021 Mar 04].
Available from: http://dx.doi.org/10.17169/refubium-6749.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Schreiber C. Prevalence and risk of infection. [Thesis]. Freie Universität Berlin; 2014. Available from: http://dx.doi.org/10.17169/refubium-6749
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Freie Universität Berlin
22.
Morgenthal, Dinah.
Prevalence of haemotropic Mycoplasma spp., Bartonella spp., Anaplasma
phagocytophilum, and Borrelia burgdorferi in cats in Northeast Germany.
Degree: 2014, Freie Universität Berlin
URL: http://dx.doi.org/10.17169/refubium-8203
► Arthropod-borne infectious agents in the cat include Bartonella (B.) henselae, Bartonella (B.) clarridgeiae, Bartonella (B.) quintana, Anaplasma (A.) phagocytophilum and Borrelia (B.) burgdorferi. Moreover infections…
(more)
▼ Arthropod-borne infectious agents in the cat include Bartonella (B.) henselae,
Bartonella (B.) clarridgeiae, Bartonella (B.) quintana,
Anaplasma (A.)
phagocytophilum and Borrelia (B.) burgdorferi. Moreover infections with
Mycoplasma (M.) haemofelis, Candidatus Mycoplasma (C. M.) turicensis and
Candidatus Mycoplasma (C. M.) haemominutum occur in the cat. The cat flea
(Ctenocephalides felis) is supposed to be the main vector for Mycoplasma spp.;
moreover, transmission via blood transfusion and saliva is possible. The aim
of this study was to evaluate the occurrence of M. haemofelis, C. M.
turicensis, C. M. haemominutum, B. henselae, B. clarridgeiae, B. quintana, A.
phagocytophilum and B. burgdorferi in healthy and ill cats in Northeast
Germany in relation to housing conditions and flea/tick exposure. 265 cats,
admitted to the Small Animal Clinic, FU Berlin between 11/2007 and 11/2008,
were included in the study (150 indoor, 99 outdoor access, 16 stray cats). 49
of the cats were healthy (e.g. blood donors) and 216 cats suffered from
various diseases. A questionnaire provided the following data: signalment,
housing environment, and previous flea/tick exposure. Serum antibody titers
against A.
phagocytophilum, B. henselae (Houston I, Marseille type), and B.
quintana were determined by an immunofluorescence test (IFT). Conventional or
real time PCR tests (EDTA blood) were used to test for A.
phagocytophilum, M.
haemofelis, C. M. turicensis, C. M. haemominutum, B. henselae and B.
clarridgeiae. Blood and buffy coat smears were prepared for each sample. Cats
tested positive (positive PCR analysis or antibody titers) were examined for
co-infections with feline leukemia virus (FeLV) or feline immunodeficiency
virus (FIV). In 19 cats (7.2%; 10 outdoor, 5 stray, 4 indoor cats) Mycoplasma
spp. DNA was detected: C. M. haemominutum (14), M. haemofelis (4) and C. M.
turicensis (3); 2 cats were co-infected with C. M. haemominutum and M.
haemofelis. Three of the cats were tested positive for the feline
immunodeficiency virus. Two of these cats (C. M. haemominutum/M. haemofelis-
coinfection; C. M. turicensis) were presented as blood donors (n=42), these
cats were not used for donation. In 12 of 19 cats (10 outdoor/2 indoor) fleas
and/or ticks had been noted. Older cats (≥ 1 year) tested significantly more
often positive (p=0.02 (χ2-test)); outdoor access cats(p=0.021 (χ2-test)) or
cats with known flea infestation (p<0.001(χ2-test)) were more often positive
for infections with Mycoplasma. Two cats with anemia (Hct 0.26 l/l, hct 0.21
l/l) were suggested to be anemic due to infection with C. M. haemominutum. All
cats were B. henselae and B. clarridgeiae PCR-negative in peripheral blood.
However, 91 of 245 cats (37.1 %) had antibody titers > 1:200 for B. henselae
(Houston I, Marseille type) and 46 (18.8 %) for B. quintana. In 32 cats (8
indoor/24 outdoor) fleas and in 39 cats (3 indoor/36 outdoor) ticks had been
noted. Older cats (≥ 1 year) were tested more often positive (antibody titers
≥ 1:200) for B. henselae (p = 0.02 (χ2-test)) and…
Advisors/Committee Members: w (gender), Univ.-Prof. Dr. Barbara Kohn (firstReferee), Univ.-Prof. Dr. Georg von Samson-Himmelstjerna (furtherReferee), Univ.-Prof. Dr. Uwe Rösler (furtherReferee).
Subjects/Keywords: ectoparasites; Bartonella spp.; Anaplasma phagocytophilum; hemotropic Mycoplasma spp.; Borrelia burgdorferi; 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
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APA (6th Edition):
Morgenthal, D. (2014). Prevalence of haemotropic Mycoplasma spp., Bartonella spp., Anaplasma
phagocytophilum, and Borrelia burgdorferi in cats in Northeast Germany. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-8203
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Morgenthal, Dinah. “Prevalence of haemotropic Mycoplasma spp., Bartonella spp., Anaplasma
phagocytophilum, and Borrelia burgdorferi in cats in Northeast Germany.” 2014. Thesis, Freie Universität Berlin. Accessed March 04, 2021.
http://dx.doi.org/10.17169/refubium-8203.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Morgenthal, Dinah. “Prevalence of haemotropic Mycoplasma spp., Bartonella spp., Anaplasma
phagocytophilum, and Borrelia burgdorferi in cats in Northeast Germany.” 2014. Web. 04 Mar 2021.
Vancouver:
Morgenthal D. Prevalence of haemotropic Mycoplasma spp., Bartonella spp., Anaplasma
phagocytophilum, and Borrelia burgdorferi in cats in Northeast Germany. [Internet] [Thesis]. Freie Universität Berlin; 2014. [cited 2021 Mar 04].
Available from: http://dx.doi.org/10.17169/refubium-8203.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Morgenthal D. Prevalence of haemotropic Mycoplasma spp., Bartonella spp., Anaplasma
phagocytophilum, and Borrelia burgdorferi in cats in Northeast Germany. [Thesis]. Freie Universität Berlin; 2014. Available from: http://dx.doi.org/10.17169/refubium-8203
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Freie Universität Berlin
23.
Gussmann, Kathrin.
Immunological control of Anaplasma phagocytophilum by murine neutrophil
granulocytes.
Degree: 2016, Freie Universität Berlin
URL: https://refubium.fu-berlin.de/handle/fub188/11025
► A. phagocytophilum is a tick-transmitted obligate intracellular Gram-negative bacterium, which replicates within its host’s neutrophil granulocytes. The human and animal granulocytic anaplasmosis often manifests as…
(more)
▼ A.
phagocytophilum is a tick-transmitted obligate intracellular Gram-negative
bacterium, which replicates within its host’s neutrophil granulocytes. The
human and animal granulocytic anaplasmosis often manifests as a nonspecific
febrile illness. The ability of A.
phagocytophilum to use particularly
neutrophil granulocytes as suitable hosts is made unique by the multitude of
their antimicrobial defense mechanisms. It is therefore of considerable
interest to more closely investigate the immunological control of A.
phagocytophilum by neutrophil granulocytes. The chosen cell culture model for
the analysis was murine Hoxb8-granulocytes, generated by retroviral
transduction with the Hoxb8-oncogene. These generated cells exhibit functional
similarity to primary cells. This technique enables a notably higher level of
purity and allowed unlimited production in comparison with ex vivo produced
murine neutrophil granulocytes. Cytokines and chemokines are presumably
involved in the pathogenesis of a A.
phagocytophilum infection. The extent up
to which the neutrophil granulocytes act as their producer during a A.
phagocytophilum infection was until now unknown. This study shows that murine
Hoxb8-granulocytes produce regulated on activation, normal T cell expressed
and secreted (RANTES), macrophage inflammatory protein-1α (MIP-1α) and tumor
necrosis factor (TNF), after stimulation with A.
phagocytophilum. In vivo
studies at the Institute of Microbiology and Hygiene had already showed that
the effector mechanisms of neutrophil granulocytes, such as nicotinamide
adenine dinucleotide phosphate (NADPH) oxidase, inducible nitric oxide
synthase (iNOS) and myeloperoxidase (MPO), were not required for pathogen
elimination in vivo. This in vitro study demonstrates that the absence of
these effector mechanisms has no influence on the growth of A.
phagocytophilum
in Hoxb8-granulocytes. This suggests that the pathogen has developed
strategies to evade these defense mechanisms. Interferon-γ (IFN-γ) is an
important modulator of the neutrophil granulocyte function. The results of
this study demonstrate that a direct IFN-γ-stimulation of murine
Hoxb8-granulocytes leads to a direct growth inhibition of A.
phagocytophilum.
Despite a noticeable induction of the iNOS mRNA-expression, due to the
infection and IFN-γ-stimulation, was this effect however iNOS-independent. In
previous studies IFN-γ-deficient mice exhibited an increased bacterial load
during the early phase of the infection, whereas the final pathogen
elimination remained unimpaired. The conclusion of this study is that a direct
IFN-γ-effect on neutrophil granulocytes is essentially involved in the in vivo
observed protective effect.
Advisors/Committee Members: w (gender), Prof. Dr. Lothar H. Wieler (firstReferee), Prof. Dr. Georg Häcker (furtherReferee), PD Dr. Jürgen Krücken (furtherReferee).
Subjects/Keywords: Rickettsiales; Anaplasma phagocytophilum; anaplasmoses; neutrophils; in vitro; deficiency; immunity; reverse transcriptase PCR; 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Gussmann, K. (2016). Immunological control of Anaplasma phagocytophilum by murine neutrophil
granulocytes. (Thesis). Freie Universität Berlin. Retrieved from https://refubium.fu-berlin.de/handle/fub188/11025
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Gussmann, Kathrin. “Immunological control of Anaplasma phagocytophilum by murine neutrophil
granulocytes.” 2016. Thesis, Freie Universität Berlin. Accessed March 04, 2021.
https://refubium.fu-berlin.de/handle/fub188/11025.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Gussmann, Kathrin. “Immunological control of Anaplasma phagocytophilum by murine neutrophil
granulocytes.” 2016. Web. 04 Mar 2021.
Vancouver:
Gussmann K. Immunological control of Anaplasma phagocytophilum by murine neutrophil
granulocytes. [Internet] [Thesis]. Freie Universität Berlin; 2016. [cited 2021 Mar 04].
Available from: https://refubium.fu-berlin.de/handle/fub188/11025.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Gussmann K. Immunological control of Anaplasma phagocytophilum by murine neutrophil
granulocytes. [Thesis]. Freie Universität Berlin; 2016. Available from: https://refubium.fu-berlin.de/handle/fub188/11025
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Freie Universität Berlin
24.
Chirek, Aleksandra.
Studies on Anaplasma phagocytophilum in dogs: clinical presentation of granulocytic anaplasmosis and significance for transfusion medicine.
Degree: 2018, Freie Universität Berlin
URL: http://dx.doi.org/10.17169/refubium-260
► A. phagocytophilum is an obligatory intracellular bacterium transmitted by various genera of Ixodes ticks. It infects neutrophils and is the causative agent of canine granulocytic…
(more)
▼ A.
phagocytophilum is an obligatory intracellular bacterium transmitted by various genera of Ixodes ticks. It infects neutrophils and is the causative agent of canine granulocytic anaplasmosis. The goals of the current retrospective studies were 1) to perform analysis of medical records of dogs naturally infected with A.
phagocytophilum, focusing on clinical signs, laboratory results, therapy and course of disease and 2) to evaluate the occurrence A.
phagocytophilum in healthy blood donor dogs and to estimate the significance for transfusion medicine.
1) Between 2006–2012 974 dogs with clinical signs suggestive for CGA were presented in the Clinic for Small Animals, Faculty of Veterinary Medicine, Freie Universität Berlin. A total of 72 dogs were PCR-positive, 63 of them fulfilled the inclusion criteria. The most common clinical signs were lethargy and reduced activity (83 %), fever (67 %), and inappetence (63 %). Thrombocytopenia was the most common laboratory abnormality (86 %), followed by increased liver enzymes activities and hyperbilirubinaemia (77 %), anaemia (70 %), hypoalbuminaemia (62 %) and leukocytosis (27 %). 44 % of 36 thrombocytopenic dogs tested for platelet-bound antibodies were positive. 59 of 61 dogs recovered, two dogs died (epileptic seizures, immune-mediated haemolytic anaemia) and two were lost for follow-up. In dogs presented with acute nonspecific clinical signs and/or immune – mediated disease, where tick exposure cannot be excluded, canine granulocytic anaplasmosis should be considered as a potential cause in areas where it is endemic.
2) Between 2006 - 2012 altogether 917 EDTA blood samples from 517 dogs were submitted for A.
phagocytophilum real-time PCR testing. 158 dogs were tested several times (2 – 11 times, median 3). The PCR test was positive for 21 of the 917 blood samples (2.3 %). Referred to the number of blood donor dogs, the prevalence was 4.06 %. Positive results were most often detected in June (n=8), May (n=5), and July (n=3), but also in five other months. None of the dogs tested PCR positive more than once. In three of 21 dogs a mild increase in rectal temperature (= 39.0 °C) was documented. Mild laboratory abnormalities were noted in eleven dogs: thrombocytopenia (n=3), leukocytosis (n=2), leukopenia (n=2), anemia (n=1) and hyperproteinemia (6 of 18 tested dogs). There was no significant difference between the PCR negative and positive blood samples with regard to laboratory abnormalities.
As altogether 2.3 % of blood samples from healthy canine blood donors were PCR positive for A.
phagocytophilum, blood donors in endemic areas should be screened for A.
phagocytophilum-DNA by PCR in blood samples all year round.
Advisors/Committee Members: female (gender), Kohn, Barbara (firstReferee), Krücken, Jürgen (furtherReferee), Arlt, Sebastian (furtherReferee).
Subjects/Keywords: dogs; Ixodes; anaplasmoses; Anaplasma phagocytophilum; real time PCR; polymerase chain reaction; clinical examination; transfusion; 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
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Record Details
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Chirek, A. (2018). Studies on Anaplasma phagocytophilum in dogs: clinical presentation of granulocytic anaplasmosis and significance for transfusion medicine. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-260
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Chirek, Aleksandra. “Studies on Anaplasma phagocytophilum in dogs: clinical presentation of granulocytic anaplasmosis and significance for transfusion medicine.” 2018. Thesis, Freie Universität Berlin. Accessed March 04, 2021.
http://dx.doi.org/10.17169/refubium-260.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Chirek, Aleksandra. “Studies on Anaplasma phagocytophilum in dogs: clinical presentation of granulocytic anaplasmosis and significance for transfusion medicine.” 2018. Web. 04 Mar 2021.
Vancouver:
Chirek A. Studies on Anaplasma phagocytophilum in dogs: clinical presentation of granulocytic anaplasmosis and significance for transfusion medicine. [Internet] [Thesis]. Freie Universität Berlin; 2018. [cited 2021 Mar 04].
Available from: http://dx.doi.org/10.17169/refubium-260.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Chirek A. Studies on Anaplasma phagocytophilum in dogs: clinical presentation of granulocytic anaplasmosis and significance for transfusion medicine. [Thesis]. Freie Universität Berlin; 2018. Available from: http://dx.doi.org/10.17169/refubium-260
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Freie Universität Berlin
25.
Liesner, Jana Marie.
Vector-borne diseases in dogs and foxes from the federal state of Brandenburg,
Germany.
Degree: 2016, Freie Universität Berlin
URL: http://dx.doi.org/10.17169/refubium-7642
► The zoonotic pathogen Dirofilaria repens, which is transmitted by mosquitos, has been found to date in eastern and southern regions of Europe. It is not…
(more)
▼ The zoonotic pathogen Dirofilaria repens, which is transmitted by mosquitos,
has been found to date in eastern and southern regions of Europe. It is not
considered to be endemic in Germany yet. In recent years, this parasite was
found repeatedly in dogs from Brandenburg and Baden-Württemberg as well as in
local mosquito species, amongst others in Brandenburg. This situation was the
starting point for the present study with the aim to examine the occurrence of
D. repens and other pathogens transmitted by fleas, ticks or mosquitoes in
dogs and red foxes from the federal state of Brandenburg. Particularly
indications concerning the epidemiological situation and a possible
endemisation of D. repens were obtained. During a time period from April 2013
to September 2014, a total of 1023 dog blood samples as well as 195 fox
spleen- and 179 fox blood samples were examined. Therefore, DNA isolation was
performed for all samples followed by different polymerase chain reactions
(PCR) to detect filarial species,
Anaplasma phagozytophilum, Candidaus
Neoehrlichia mikurensis, Ehrlichia canis, Rickettsia spp. and piroplasms.
Filarial species were detected in six (0.6%) out of 1023 dogs, including two
infected with D. repens, two harbouring Dirofilaria immitis and two other with
Acanthocheilonema reconditum. One of the two positive D. repens dogs
originated from an animal shelter in Brandenburg, but for the other one origin
remained unknown. Interestingly, both ITS-1 D. repens sequences showed a 100%
identity to a D. repens sample obtained from a Japanese woman that travelled
in Europe. They also showed 97% and 98% identity to a new species Dirofilaria
hongkongensis that was so far only described from HongKong, China. An endemic
occurence of D. repens in dogs or foxes in the federal state of Brandenburg
was not confirmed in this study. Nevertheless, the risk remains high due to
previous findings of the pathogen in mosquitoes and dogs. A.
phagocytophilum
was found in 15 dogs (1.5%), Candidatus N. mikurensis in three dogs (0.3%) and
E. canis in one dog (0.1%). The E. canis positive dog (no. 117) also had a co-
infection with D. repens. Rickettsia spp. were found with a frequency of 0.8%
in dogs, confirming seven samples as Rickettsia raoultii and one as Rickettsia
felis. In 85 (47.5 %) out of 179 fox blood samples piroplasms were found and
in 10 of 11 which were furthermore examined by gene sequencing, Theileria
annae was identified. Another positive sample showed cross-reactivity to
Coccidiae in the PCR and was then confirmed as Sarcocystis arctica. Thus, the
red fox was confirmed as a reservoir host for T. annae, but fox samples were
negative for all the other pathogens of interest in the present study. In one
dog (0.1 %), Babesia canis was detected but there was no further information
about its origin. A questionnaire for owners of patients should provide
information about the origin, past journeys and parasite prevention measures.
The evaluation identified a high proportion of 74.2 % (n = 233) of the dogs
that were not protected…
Advisors/Committee Members: w (gender), Univ.-Prof. Dr. Georg von Samson-Himmelstjerna (firstReferee), Univ.-Prof. Dr. Barbara Kohn (furtherReferee), Univ.-Prof. Dr. Marcus Fulde (furtherReferee).
Subjects/Keywords: dogs; foxes; Culicidae; Dirofilaria repens; Anaplasma phagocytophilum; Ehrlichia canis; Dirofilaria immitis; 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Liesner, J. M. (2016). Vector-borne diseases in dogs and foxes from the federal state of Brandenburg,
Germany. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-7642
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Liesner, Jana Marie. “Vector-borne diseases in dogs and foxes from the federal state of Brandenburg,
Germany.” 2016. Thesis, Freie Universität Berlin. Accessed March 04, 2021.
http://dx.doi.org/10.17169/refubium-7642.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Liesner, Jana Marie. “Vector-borne diseases in dogs and foxes from the federal state of Brandenburg,
Germany.” 2016. Web. 04 Mar 2021.
Vancouver:
Liesner JM. Vector-borne diseases in dogs and foxes from the federal state of Brandenburg,
Germany. [Internet] [Thesis]. Freie Universität Berlin; 2016. [cited 2021 Mar 04].
Available from: http://dx.doi.org/10.17169/refubium-7642.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Liesner JM. Vector-borne diseases in dogs and foxes from the federal state of Brandenburg,
Germany. [Thesis]. Freie Universität Berlin; 2016. Available from: http://dx.doi.org/10.17169/refubium-7642
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Freie Universität Berlin
26.
Lazzerini, Kali.
Testing of dogs with meningitis and meningoencephalitis of unknown etiology
for vector-transmitted microorganisms.
Degree: 2016, Freie Universität Berlin
URL: http://dx.doi.org/10.17169/refubium-8317
► In many cases of inflammatory diseases of the central nervous system in dogs, no aetiological infectious agent can be found. These inflammatory conditions are thus…
(more)
▼ In many cases of inflammatory diseases of the central nervous system in dogs,
no aetiological infectious agent can be found. These inflammatory conditions
are thus named inflammations of unknown aetiology. Results of
immunpathological studies imply that an antigen may trigger an autoimmune
response (Hit-and-Run-Hypothesis). Serum was analyzed for antibodies against
vector-transmitted pathogens and blood and cerebrospinal fluid for DNA of such
infectious agents in order to further define the role of CVBD-agents in the
aetiology of meningitis and meningoencephalitis of unknown aetiology in dogs
in Germany. 66 client-owned dogs were included in the prospective multicenter
study between december 2009 and november 2011. They were classified in three
groups: a.) control-group with dogs with non-inflammatory CNS-disease (e.g.
intervertebral disc disease, n=21) (trauma group) b.) dogs with
meningoencephalitis of unknown aetiology (MUE) (n=22) c.) dogs with steroid-
responsive meningitis-arteritis (SRMA) (n=23) PCR was performed in blood and
cerebrospinal fluid to detect A.
phagocytophilum, E. canis (for dogs that
stayed in an endemic area or dogs with unknown past, n=28) and Bartonella spp.
Serological assays targeted E. canis (IFAT), TBEV (ELISA) and Borrelia
burgdorferi sensu lato antibodies (IFAT and C6-ELISA for patients with
elevated antibody titers in IFAT). Group comparison was done with non
parametric tests, using the statistical software SPSS 17.0 for windows, SPSS
Inc., USA. No DNA was found in cerebrospinal fluid. In 4 dogs of the SRMA-
group, DNA of A.
phagocytophilum was found in blood. Serological and PCR
analysis for E. canis were negative in all dogs in blood and serum. No
elevated antibody-titers against TBEV were measured in any dog. B. henselae
DNA was detected in blood of 1 dog of the SRMA-group. 14 dogs had an elevated
antibody titer (> 1:128) against B. burgdorferi sensu lato (IFAT). There were
no significant differences between the 3 groups. In two dogs of the SRMA-group
and in one dog of the MUE-group, an elevated C6-titer was detected via
C6-ELISA (> 10 U/ml). DNA of Pasteurellaceae was detected with eubacterial PCR
in CSF of 3 dogs of the trauma group, which may be due to a contamination of
the samples. No correlation could be determined between the presence of E.
canis DNA or elevated antibody-titers against E. canis or TBEV and
inflammatory CNS diseases. 17 % of dogs with SRMA had positive PCR results for
A.
phagocytophilum. A.
phagocytophilum may play a role as trigger of a
secondary immunopathy. It remains unclear whether the positive test results
for Bartonella DNA and Borrelia burgdorferi sensu lato antibodies are
clinically relevant.
Advisors/Committee Members: w (gender), Univ.-Prof. Dr. Barbara Kohn (firstReferee), Prof. Dr. Andrea Tipold (furtherReferee), PD Dr. Jürgen Krücken (furtherReferee).
Subjects/Keywords: Anaplasma phagocytophilum; central nervous system; cerebrospinal fluid; inflammation; meningoencephalitis; meningitis; 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Lazzerini, K. (2016). Testing of dogs with meningitis and meningoencephalitis of unknown etiology
for vector-transmitted microorganisms. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-8317
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Lazzerini, Kali. “Testing of dogs with meningitis and meningoencephalitis of unknown etiology
for vector-transmitted microorganisms.” 2016. Thesis, Freie Universität Berlin. Accessed March 04, 2021.
http://dx.doi.org/10.17169/refubium-8317.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Lazzerini, Kali. “Testing of dogs with meningitis and meningoencephalitis of unknown etiology
for vector-transmitted microorganisms.” 2016. Web. 04 Mar 2021.
Vancouver:
Lazzerini K. Testing of dogs with meningitis and meningoencephalitis of unknown etiology
for vector-transmitted microorganisms. [Internet] [Thesis]. Freie Universität Berlin; 2016. [cited 2021 Mar 04].
Available from: http://dx.doi.org/10.17169/refubium-8317.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Lazzerini K. Testing of dogs with meningitis and meningoencephalitis of unknown etiology
for vector-transmitted microorganisms. [Thesis]. Freie Universität Berlin; 2016. Available from: http://dx.doi.org/10.17169/refubium-8317
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Guelph
27.
Smith, Kathryn.
Powassan Virus and Other Tick-Borne Pathogens from Wildlife and Companion Animals in Southern Ontario.
Degree: MS, Department of Pathobiology, 2017, University of Guelph
URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/11594
► The objectives of the present research were to survey potential vertebrate host and tick species for evidence of infections with POWV and other arthropod-borne pathogens…
(more)
▼ The objectives of the present research were to survey potential vertebrate host and tick species for evidence of infections with POWV and other arthropod-borne pathogens including West Nile virus, Heartland virus,
Anaplasma phagocytophilum, Babesia microti, Borrelia miyamotoi, B. burgdorferi and Ehrlichia chaffeensis in mammalian wildlife, dogs and ticks in southern Ontario. During the summers of 2015-2016, ticks and tissues were collected from carcasses of free-ranging, medium-sized mammals; blood and ticks were collected from live-trapped mammals, and ticks removed from dogs were collected from local veterinary clinics. Albeit rarely, evidence of the presence of POWV was found both by polymerase chain reaction in ticks and serological tests in groundhogs (Marmota monax) and striped skunks (Mephitis mephitis). Evidence of A.
phagocytophilum and B. burgdorferi was also rarely detected in Ixodes scapularis ticks. These findings emphasize the importance of both broad and targeted surveillance strategies for investigating emerging tick-borne diseases.
Advisors/Committee Members: Nemeth, Nicole M. (advisor).
Subjects/Keywords: Anaplasma phagocytophilum; Borrelia burgdorferi; dog; Ixodes scapularis; Ontario; raccoon; tick; Ixodes texanus; tick-borne pathogen; wildlife; arbovirus; Heartland virus; Powassan virus; West Nile virus
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APA (6th Edition):
Smith, K. (2017). Powassan Virus and Other Tick-Borne Pathogens from Wildlife and Companion Animals in Southern Ontario. (Masters Thesis). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/11594
Chicago Manual of Style (16th Edition):
Smith, Kathryn. “Powassan Virus and Other Tick-Borne Pathogens from Wildlife and Companion Animals in Southern Ontario.” 2017. Masters Thesis, University of Guelph. Accessed March 04, 2021.
https://atrium.lib.uoguelph.ca/xmlui/handle/10214/11594.
MLA Handbook (7th Edition):
Smith, Kathryn. “Powassan Virus and Other Tick-Borne Pathogens from Wildlife and Companion Animals in Southern Ontario.” 2017. Web. 04 Mar 2021.
Vancouver:
Smith K. Powassan Virus and Other Tick-Borne Pathogens from Wildlife and Companion Animals in Southern Ontario. [Internet] [Masters thesis]. University of Guelph; 2017. [cited 2021 Mar 04].
Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/11594.
Council of Science Editors:
Smith K. Powassan Virus and Other Tick-Borne Pathogens from Wildlife and Companion Animals in Southern Ontario. [Masters Thesis]. University of Guelph; 2017. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/11594
University of Georgia
28.
Manangan, Jamie Sara.
Spatial analysis of the distributions of two tick-borne bacteria, Anaplasma phagocytophilum and Ehrlichia chaffeensis, in the Mississippi Alluvial Valley.
Degree: 2014, University of Georgia
URL: http://hdl.handle.net/10724/23677
► Human monocytotropic ehrlichiosis (HME), caused by the bacterium Ehrlichia chaffeensis, and human granulocytic anaplasmosis (HGA), caused by the bacterium Anaplasma phagocytophilum, are two emerging tick-borne…
(more)
▼ Human monocytotropic ehrlichiosis (HME), caused by the bacterium Ehrlichia chaffeensis, and human granulocytic anaplasmosis (HGA), caused by the bacterium Anaplasma phagocytophilum, are two emerging tick-borne zoonoses of concern. The
primary objective of this research was to determine the influences of the physical environment, land cover composition, and landscape heterogeneity on the spatial distributions of E. chaffeensis and A. phagocytophilum at multiple spatial scales in the
Mississippi Alluvial Valley. Logistic regression models were developed using three different strategies (a priori, exploratory, and spatial extent). Ecological predictor variables used to develop models included deer density, elevation, land cover,
normalized difference vegetation indices, hydrology, and soil moisture. Presence or absence of bacteria was measured using white-tailed deer (Odocoileus virginianus) serology samples collected in the period of 1981-2005. This research indicates that land
cover composition and configuration (measures of habitat availability and connectivity for vector or host species) and probability of flooding (because of impacts on vector populations) influence pathogen distributions in the MAV.
Subjects/Keywords: Anaplasma phagocytophilum; Ehrlichia chaffeensis; Amblyomma americanum; Ixodes scapularis; white-tailed deer; tick-borne disease; landscape ecology; geographic information systems; mississippi alluvial valley
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APA ·
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APA (6th Edition):
Manangan, J. S. (2014). Spatial analysis of the distributions of two tick-borne bacteria, Anaplasma phagocytophilum and Ehrlichia chaffeensis, in the Mississippi Alluvial Valley. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/23677
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Manangan, Jamie Sara. “Spatial analysis of the distributions of two tick-borne bacteria, Anaplasma phagocytophilum and Ehrlichia chaffeensis, in the Mississippi Alluvial Valley.” 2014. Thesis, University of Georgia. Accessed March 04, 2021.
http://hdl.handle.net/10724/23677.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Manangan, Jamie Sara. “Spatial analysis of the distributions of two tick-borne bacteria, Anaplasma phagocytophilum and Ehrlichia chaffeensis, in the Mississippi Alluvial Valley.” 2014. Web. 04 Mar 2021.
Vancouver:
Manangan JS. Spatial analysis of the distributions of two tick-borne bacteria, Anaplasma phagocytophilum and Ehrlichia chaffeensis, in the Mississippi Alluvial Valley. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/10724/23677.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Manangan JS. Spatial analysis of the distributions of two tick-borne bacteria, Anaplasma phagocytophilum and Ehrlichia chaffeensis, in the Mississippi Alluvial Valley. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/23677
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Georgia
29.
DUGAN, VIVIEN GRACE.
White-tailed deer and raccoons as hosts for selected zoonotic tick-borne rickettsiae.
Degree: 2014, University of Georgia
URL: http://hdl.handle.net/10724/22635
► The central aim of this research was to develop a more complete epidemiologic understanding of Anaplasma phagocytophilum, Ehrlichia chaffeensis, Ehrlichia canis, and Ehrlichia ewingii in…
(more)
▼ The central aim of this research was to develop a more complete epidemiologic understanding of Anaplasma phagocytophilum, Ehrlichia chaffeensis, Ehrlichia canis, and Ehrlichia ewingii in the southeastern United States. These bacterial cause
clinical infection in humans, dogs and/or horses, are transmitted by three different genera of ticks, and involve wildlife in their epidemiology. Raccoons (Procyon lotor) and opossums (Didelphis virginianus) from Georgia were examined for their role in
the maintenance of these ehrlichial and anaplasmal species. Raccoons were positive for antibodies to E. chaffeensis, E. canis, and to a lesser extent, A. phagocytophilum and blood from one raccoon was PCR positive for E. canis. All raccoons were PCR
negative for E. chaffeensis, E. ewingii, and A. phagocytophilum; however, a novel Ehrlichia-like gene fragment was amplified from over half of the raccoons. In contrast, opossums were negative by all assays. These findings suggest that raccoons, but not
opossums, may be involved in the epidemiology of one or more of these pathogens. White-tailed deer (WTD; Odocoileus virginianus) were evaluated for their suitability as natural sentinels for discerning the geographic distribution of A. phagocytophilum
across 19 states. This goal was accomplished by testing 2,666 WTD from 507 populations for A. phagocytophilum-reactive antibodies, with confirmatory PCR testing and in vivo xenodiagnostic cultures. WTD met crucial standards as sentinels and evaluation of
field data indicated that WTD antibodies reflect infection, small sample sizes were adequate for accurate surveillance, and A. phagocytophilum infection in WTD was correlated with the presence of I. scapularis. Serologic WTD data were used in two
different modeling analyses to predict the distribution of A. phagocytophilum across 19 states. Kriging and logistic regression model analyses disclosed moderately high (> 64%) accuracy, sensitivity, and specificity and the projected distribution
conformed to human case data available for this region. Additionally, analyses determined significant ecologic variables associated with the presence of A. phagocytophilum. This study demonstrated that WTD would be an effective surveillance species and
represent a feasible alternative to human surveillance in predicting the geographic distribution of locations where there is a risk of A. phagocytophilum infection.
Subjects/Keywords: Anaplasma phagocytophilum; white-tailed deer; Odocoileus virginianus; surveillance system; sentinels; epidemiology; geographic information system; Ixodes scapularis; raccoons; Procyon lotor; Ehrlichia chaffeensis; Ehrlichia canis; Ehrlichia ewingii; Ehrlichia sp.
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APA ·
Chicago ·
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CSE |
Export
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Manager
APA (6th Edition):
DUGAN, V. G. (2014). White-tailed deer and raccoons as hosts for selected zoonotic tick-borne rickettsiae. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/22635
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
DUGAN, VIVIEN GRACE. “White-tailed deer and raccoons as hosts for selected zoonotic tick-borne rickettsiae.” 2014. Thesis, University of Georgia. Accessed March 04, 2021.
http://hdl.handle.net/10724/22635.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
DUGAN, VIVIEN GRACE. “White-tailed deer and raccoons as hosts for selected zoonotic tick-borne rickettsiae.” 2014. Web. 04 Mar 2021.
Vancouver:
DUGAN VG. White-tailed deer and raccoons as hosts for selected zoonotic tick-borne rickettsiae. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/10724/22635.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
DUGAN VG. White-tailed deer and raccoons as hosts for selected zoonotic tick-borne rickettsiae. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/22635
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
30.
Niu, Hua.
The Role of Cellular Autophagy and Type IV Secretion System
in <i>Anaplasma phagocytophilum</i> Infection.
Degree: PhD, Veterinary Biosciences, 2008, The Ohio State University
URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1211307210
► Human granulocytic anaplasmosis (HGA), an emerging tick-borne zoonosis is caused by a gram-negative, obligatory intracellular bacterium, <i>Anaplasma phagocytophilum</i>. <i>A. phagocytophilum</i> has the remarkable ability…
(more)
▼ Human granulocytic anaplasmosis (HGA), an
emerging tick-borne zoonosis is caused by a gram-negative,
obligatory intracellular bacterium, <i>
Anaplasma
phagocytophilum</i>. <i>A.
phagocytophilum</i>
has the remarkable ability to inhibit the spontaneous apoptosis of
neutrophils, block the production of reactive oxygen intermediates,
and replicate in membrane-bound inclusions in the cytoplasm of
neutrophils. However, the <i>A.
phagocytophilum</i>
inclusions have not been fully characterized, and bacterial factors
contributing to these phenomena remain unknown. In this study, we
studied several molecular aspects of <i>A.
phagocytophilum</i> pathogenesis. (1)
Characterization of <i>A.
phagocytophilum</i>
replicative inclusions. We demonstrated that <i>A.
phagocytophilum</i> replicative inclusions had the
characteristic of early autophagosomes, as shown by the presence of
autophagosome markers, LC3 and double lipid bilayer membrane in the
<i>A.
phagocytophilum</i> inclusions. Furthermore our
data suggested that autophagy enhanced <i>A.
phagocytophilum</i> replication instead of inhibiting its
growth. (2) Investigation of the expression of
genes encoding type IV secretion system apparatus in <i>A.
phagocytophilum</i>. We found the expression of
<i>virB6</i> and <i>virB9</i> was
up-regulated during the bacterial growth in human neutrophils.
Furthermore, differential VirB9 expression was shown to associate
with the binding of <i>A.
phagocytophilum</i> to
neutrophils, and prevention of internalized bacteria from being
delivered to lysosomes. (3) Identification of a
novel substrate of type IV secretion system in <i>A.
phagocytophilum</i>. A novel substrate, Ats-1 was identified
by bacterial two-hybrid screening. We demonstrated that Ats-1
targeted mitochondria of host cells, and inhibited cell apoptosis.
(4) Development of a reporter system in
<i>A.
phagocytophilum</i> to detect substrates of type
IV secretion system. We made some progress to achieve the goal by
constructing plasmid harboring reporter-substrate fusion
gene.
Advisors/Committee Members: Rikihisa, Yasuko (Advisor), Oglesbee, Michael (Committee Chair).
Subjects/Keywords: Microbiology; Anaplasma phagocytophilum; Type IV Secretion System; Autophagy
…1
2. Subversion of cellular autophagy by Anaplasma phagocytophilum .…......
15
2.1… …Anaplasma
phagocytophilum in human leucocytes is associated with differential binding
and… …Discussion
50
51
53
61
68
viii
4. Anaplasma phagocytophilum ats-1… …infection with Anaplasma phagocytophilum. It was first identified in a Wisconsin patient
in 1990… …Dumler et al., 2005).
Anaplasma phagocytophilum
A. phagocytophilum, the etiologic agent…
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Niu, H. (2008). The Role of Cellular Autophagy and Type IV Secretion System
in <i>Anaplasma phagocytophilum</i> Infection. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1211307210
Chicago Manual of Style (16th Edition):
Niu, Hua. “The Role of Cellular Autophagy and Type IV Secretion System
in <i>Anaplasma phagocytophilum</i> Infection.” 2008. Doctoral Dissertation, The Ohio State University. Accessed March 04, 2021.
http://rave.ohiolink.edu/etdc/view?acc_num=osu1211307210.
MLA Handbook (7th Edition):
Niu, Hua. “The Role of Cellular Autophagy and Type IV Secretion System
in <i>Anaplasma phagocytophilum</i> Infection.” 2008. Web. 04 Mar 2021.
Vancouver:
Niu H. The Role of Cellular Autophagy and Type IV Secretion System
in <i>Anaplasma phagocytophilum</i> Infection. [Internet] [Doctoral dissertation]. The Ohio State University; 2008. [cited 2021 Mar 04].
Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1211307210.
Council of Science Editors:
Niu H. The Role of Cellular Autophagy and Type IV Secretion System
in <i>Anaplasma phagocytophilum</i> Infection. [Doctoral Dissertation]. The Ohio State University; 2008. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1211307210
◁ [1] [2] ▶
. 
Open Access Theses and Dissertations
