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University of Helsinki
1.
D'Ulivo, Lucia.
Capillary electrochromatography: a versatile instrumental technique for nanodomain interaction studies.
Degree: Department of Chemistry, 2010, University of Helsinki
URL: http://hdl.handle.net/10138/21019 
► This doctoral thesis describes the development of a miniaturized capillary electrochromatography (CEC) technique suitable for the study of interactions between various nanodomains of biological importance.…
(more)
▼ This doctoral thesis describes the development of a miniaturized capillary electrochromatography (CEC) technique suitable for the study of interactions between various nanodomains of biological importance. The particular focus of the study was low-density lipoprotein (LDL) particles and their interaction with components of the extracellular matrix (ECM). LDL transports cholesterol to the tissues through the blood circulation, but when the LDL level becomes too high the particles begin to permeate and accumulate in the arteries. Through binding sites on apolipoprotein B-100 (apoB-100), LDL interacts with components of the ECM, such as proteoglycans (PGs) and collagen, in what is considered the key mechanism in the retention of lipoproteins and onset of atherosclerosis. Hydrolytic enzymes and oxidizing agents in the ECM may later successively degrade the LDL surface. Metabolic diseases such as diabetes may provoke damage of the ECM structure through the non-enzymatic reaction of glucose with collagen. In this work, fused silica capillaries of 50 micrometer i.d. were successfully coated with LDL and collagen, and steroids and apoB-100 peptide fragments were introduced as model compounds for interaction studies. The LDL coating was modified with copper sulphate or hydrolytic enzymes, and the interactions of steroids with the native and oxidized lipoproteins were studied. Lipids were also removed from the LDL particle coating leaving behind an apoB-100 surface for further studies. The development of collagen and collagen decorin coatings was helpful in the elucidation of the interactions of apoB-100 peptide fragments with the primary ECM component, collagen. Furthermore, the collagen I coating provided a good platform for glycation studies and for clarification of LDL interactions with native and modified collagen. All methods developed are inexpensive, requiring just small amounts of biomaterial. Moreover, the experimental conditions in CEC are easily modified, and the analyses can be carried out in a reasonable time frame. Other techniques were employed to support and complement the CEC studies. Scanning electron microscopy and atomic force microscopy provided crucial visual information about the native and modified coatings. Asymmetrical flow field-flow fractionation enabled size measurements of the modified lipoproteins. Finally, the CEC results were exploited to develop new sensor chips for a continuous flow quartz crystal microbalance technique, which provided complementary information about LDL ECM interactions. This thesis demonstrates the potential of CEC as a valuable and flexible technique for surface interaction studies. Further, CEC can serve as a novel microreactor for the in situ modification of LDL and collagen coatings. The coatings developed in this study provide useful platforms for a diversity of future investigations on biological nanodomains.
Not available
Subjects/Keywords: analytical Chemistry; analytical Chemistry
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APA (6th Edition):
D'Ulivo, L. (2010). Capillary electrochromatography: a versatile instrumental technique for nanodomain interaction studies. (Doctoral Dissertation). University of Helsinki. Retrieved from http://hdl.handle.net/10138/21019
Chicago Manual of Style (16th Edition):
D'Ulivo, Lucia. “Capillary electrochromatography: a versatile instrumental technique for nanodomain interaction studies.” 2010. Doctoral Dissertation, University of Helsinki. Accessed February 16, 2019.
http://hdl.handle.net/10138/21019.
MLA Handbook (7th Edition):
D'Ulivo, Lucia. “Capillary electrochromatography: a versatile instrumental technique for nanodomain interaction studies.” 2010. Web. 16 Feb 2019.
Vancouver:
D'Ulivo L. Capillary electrochromatography: a versatile instrumental technique for nanodomain interaction studies. [Internet] [Doctoral dissertation]. University of Helsinki; 2010. [cited 2019 Feb 16].
Available from: http://hdl.handle.net/10138/21019.
Council of Science Editors:
D'Ulivo L. Capillary electrochromatography: a versatile instrumental technique for nanodomain interaction studies. [Doctoral Dissertation]. University of Helsinki; 2010. Available from: http://hdl.handle.net/10138/21019
Iowa State University
2.
Klein, Adam T.
Advancements in mass spectrometry for biological samples: Protein chemical cross-linking and metabolite analysis of plant tissues.
Degree: 2015, Iowa State University
URL: https://lib.dr.iastate.edu/etd/14803
► This thesis presents work on advancements and applications of methodology for the analysis of biological samples using mass spectrometry. Included in this work are improvements…
(more)
▼ This thesis presents work on advancements and applications of methodology for the analysis of biological samples using mass spectrometry. Included in this work are improvements to chemical cross-linking mass spectrometry (CXMS) for the study of protein structures and mass spectrometry imaging and quantitative analysis to study plant metabolites. Applications include using matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) to further explore metabolic heterogeneity in plant tissues and chemical interactions at the interface between plants and pests. Additional work was focused on developing liquid chromatography-mass spectrometry (LC-MS) methods to investigate metabolites associated with plant-pest interactions.
The first chapter includes an introduction into CXMS and MS-based metabolite analysis and the sixth and final chapter includes a brief summary of the work and future directions based on the work presented here.
The second chapter discusses the limitations of chemical cross-linking mass spectrometry, and steps taken to overcome these. This includes the incorporation of hydrogen-deuterium exchange mass spectrometry (HDX/MS) to identify the presence of structural distortion caused by the chemical cross-linking reaction. The use of extracted ion chromatograms (XIC) to increase cross-linking detection efficiency is also discussed.
The third chapter focuses on the application of MALDI-MSI to investigate seedling maize leaf tissues. Metabolites related to the photosynthetic process are compared at different locations along the leaves of two inbred genotypes. The localization and relative abundances of phosphatidylglycerols (PG) and sulfoquinovosyl diacylglycerols (SQDG) are described in detail.
The fourth chapter illustrates how imprinting MSI can be used to study the chemical interface between plants and pests. The interaction between soybean aphids and soybean leaves was studied using MALDI-MSI on imprints of infested leaves. These experiments revealed the spatial distribution of numerous metabolites, including compounds involved in the plant-pest interaction.
The fifth chapter discusses the development of a methodology to determine quaternary ammonium compounds (QACs) in plant samples. Hydrophilic interaction liquid chromatography (HILIC) mass spectrometry was used to quantitatively and qualitatively analyze common bean seed exudates. This work was focused on QACs that bacteria are known to uptake for osmoprotection and nutritional benefits.
Subjects/Keywords: Analytical Chemistry; Analytical Chemistry
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APA (6th Edition):
Klein, A. T. (2015). Advancements in mass spectrometry for biological samples: Protein chemical cross-linking and metabolite analysis of plant tissues. (Thesis). Iowa State University. Retrieved from https://lib.dr.iastate.edu/etd/14803
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Klein, Adam T. “Advancements in mass spectrometry for biological samples: Protein chemical cross-linking and metabolite analysis of plant tissues.” 2015. Thesis, Iowa State University. Accessed February 16, 2019.
https://lib.dr.iastate.edu/etd/14803.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Klein, Adam T. “Advancements in mass spectrometry for biological samples: Protein chemical cross-linking and metabolite analysis of plant tissues.” 2015. Web. 16 Feb 2019.
Vancouver:
Klein AT. Advancements in mass spectrometry for biological samples: Protein chemical cross-linking and metabolite analysis of plant tissues. [Internet] [Thesis]. Iowa State University; 2015. [cited 2019 Feb 16].
Available from: https://lib.dr.iastate.edu/etd/14803.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Klein AT. Advancements in mass spectrometry for biological samples: Protein chemical cross-linking and metabolite analysis of plant tissues. [Thesis]. Iowa State University; 2015. Available from: https://lib.dr.iastate.edu/etd/14803
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
3.
Jacobs, Jeneé L.
Diagnostic studies of ion beam formation in inductively coupled plasma mass spectrometry with the collision reaction interface.
Degree: 2015, Iowa State University
URL: https://lib.dr.iastate.edu/etd/14814
► This dissertation describes a variety of studies focused on the plasma and the ion beam in inductively coupled plasma mass spectrometry (ICP-MS). The ability to…
(more)
▼ This dissertation describes a variety of studies focused on the plasma and the ion beam in inductively coupled plasma mass spectrometry (ICP-MS). The ability to use ICP-MS for measurements of trace elements in samples requires the analytes to be efficiently ionized. Updated ionization efficiency tables are discussed for ionization temperatures of 6500 K and 7000 K with an electron density of 1 1015 cm-3. These values are reflective of the current operating parameters of ICP-MS instruments. Calculations are also discussed for doubly charged (M2+) ion formation, neutral metal oxide (MO) ionization, and metal oxide (MO+) ion dissociation for similar plasma temperature values. Ionization efficiency results for neutral MO molecules in the ICP have not been reported previously.
The collision reaction interface (CRI) removes polyatomic ions in ICP-MS by introducing a collision or reaction gas directly into the plasma expansion as ions are extracted into the mass spectrometer. The main gases implemented in CRI are helium and hydrogen. Experiments are described for the determination of the collision cross sections of singly charged cations by employing helium in a hexapole collision cell. Based on those determined cross sections, general characteristics of the CRI collision region are determined and discussed using helium as a collision gas. One concern with implementing H2 as a reaction gas via the CRI is the possible formation of monohydride ions (MH+, M = atomic ion). The formation of MH+ ions and the influence of increasing H2 gas flow through the CRI skimmer cone are discussed to provide insight into the source of MH+ ion formation in the CRI and plasma expansion regions.
Subjects/Keywords: Analytical Chemistry; Analytical Chemistry
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APA (6th Edition):
Jacobs, J. L. (2015). Diagnostic studies of ion beam formation in inductively coupled plasma mass spectrometry with the collision reaction interface. (Thesis). Iowa State University. Retrieved from https://lib.dr.iastate.edu/etd/14814
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Jacobs, Jeneé L. “Diagnostic studies of ion beam formation in inductively coupled plasma mass spectrometry with the collision reaction interface.” 2015. Thesis, Iowa State University. Accessed February 16, 2019.
https://lib.dr.iastate.edu/etd/14814.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Jacobs, Jeneé L. “Diagnostic studies of ion beam formation in inductively coupled plasma mass spectrometry with the collision reaction interface.” 2015. Web. 16 Feb 2019.
Vancouver:
Jacobs JL. Diagnostic studies of ion beam formation in inductively coupled plasma mass spectrometry with the collision reaction interface. [Internet] [Thesis]. Iowa State University; 2015. [cited 2019 Feb 16].
Available from: https://lib.dr.iastate.edu/etd/14814.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Jacobs JL. Diagnostic studies of ion beam formation in inductively coupled plasma mass spectrometry with the collision reaction interface. [Thesis]. Iowa State University; 2015. Available from: https://lib.dr.iastate.edu/etd/14814
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Florida International University
4.
Cerreta, Michelle.
The Creation and Evaluation of Novel Canine Training Aids for Cocaine Using Molecularly Encapsulated Sol-Gel Polymers and an Investigation of Canine Field Accuracy.
Degree: PhD, Chemistry, 2015, Florida International University
URL: http://digitalcommons.fiu.edu/etd/1901
;
10.25148/etd.FI15032132
;
FI15032132
► Biological detectors, such as canines, are valuable tools used for the rapid identification of illicit materials. However, recent increased scrutiny over the reliability, field…
(more)
▼ Biological detectors, such as canines, are valuable tools used for the rapid identification of illicit materials. However, recent increased scrutiny over the reliability, field accuracy, and the capabilities of each detection canine is currently being evaluated in the legal system. For example, the Supreme Court case, State of Florida v. Harris, discussed the need for continuous monitoring of canine abilities, thresholds, and search capabilities. As a result, the fallibility of canines for detection was brought to light, as well as a need for further research and understanding of canine detection. This study is two-fold, as it looks to not only create new training aids for canines that can be manipulated for dissipation control, but also investigates canine field accuracy to objects with similar odors to illicit materials.
It was the goal of this research to improve upon current canine training aid mimics. Sol-gel polymer training aids, imprinted with the active odor of cocaine, were developed. This novel training aid improved upon the longevity of currently existing training aids, while also provided a way to manipulate the polymer network to alter the dissipation rate of the imprinted active odors. The manipulation of the polymer network could allow handlers to control the abundance of odors presented to their canines, familiarizing themselves to their canine’s capabilities and thresholds, thereby increasing the canines’ strength in court.
The field accuracy of detection canines was recently called into question during the Supreme Court case, State of Florida v. Jardines, where it was argued that if cocaine’s active odor, methyl benzoate, was found to be produced by the popular landscaping flower, snapdragons, canines will false alert to said flowers. Therefore, snapdragon flowers were grown and tested both in the laboratory and in the field to determine the odors produced by snapdragon flowers; the persistence of these odors once flowers have been cut; and whether detection canines will alert to both growing and cut flowers during a blind search scenario. Results revealed that although methyl benzoate is produced by snapdragon flowers, certified narcotics detection canines can distinguish cocaine’s odor profile from that of snapdragon flowers and will not alert.
Advisors/Committee Members: Kenneth G. Furton, DeEtta Mills, Watson Lees, Anthony DeCaprio, Yong Cai.
Subjects/Keywords: analytical chemistry; chemistry; Analytical Chemistry; Chemistry
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APA (6th Edition):
Cerreta, M. (2015). The Creation and Evaluation of Novel Canine Training Aids for Cocaine Using Molecularly Encapsulated Sol-Gel Polymers and an Investigation of Canine Field Accuracy. (Doctoral Dissertation). Florida International University. Retrieved from http://digitalcommons.fiu.edu/etd/1901 ; 10.25148/etd.FI15032132 ; FI15032132
Chicago Manual of Style (16th Edition):
Cerreta, Michelle. “The Creation and Evaluation of Novel Canine Training Aids for Cocaine Using Molecularly Encapsulated Sol-Gel Polymers and an Investigation of Canine Field Accuracy.” 2015. Doctoral Dissertation, Florida International University. Accessed February 16, 2019.
http://digitalcommons.fiu.edu/etd/1901 ; 10.25148/etd.FI15032132 ; FI15032132.
MLA Handbook (7th Edition):
Cerreta, Michelle. “The Creation and Evaluation of Novel Canine Training Aids for Cocaine Using Molecularly Encapsulated Sol-Gel Polymers and an Investigation of Canine Field Accuracy.” 2015. Web. 16 Feb 2019.
Vancouver:
Cerreta M. The Creation and Evaluation of Novel Canine Training Aids for Cocaine Using Molecularly Encapsulated Sol-Gel Polymers and an Investigation of Canine Field Accuracy. [Internet] [Doctoral dissertation]. Florida International University; 2015. [cited 2019 Feb 16].
Available from: http://digitalcommons.fiu.edu/etd/1901 ; 10.25148/etd.FI15032132 ; FI15032132.
Council of Science Editors:
Cerreta M. The Creation and Evaluation of Novel Canine Training Aids for Cocaine Using Molecularly Encapsulated Sol-Gel Polymers and an Investigation of Canine Field Accuracy. [Doctoral Dissertation]. Florida International University; 2015. Available from: http://digitalcommons.fiu.edu/etd/1901 ; 10.25148/etd.FI15032132 ; FI15032132
University of California, San Diego
5.
Staley, Patrick Andrew.
The Electrochemistry of Quinones in Aprotic Solvents.
Degree: 2017, University of California, San Diego
URL: http://pqdtopen.proquest.com/#viewpdf?dispub=10249059
► Quinones are electroactive organic molecules that are used by biological systems as electron shuttles, are used by humans as dyes, pharmaceuticals, reactants, and catalysts,…
(more)
▼ Quinones are electroactive organic molecules that are used by biological systems as electron shuttles, are used by humans as dyes, pharmaceuticals, reactants, and catalysts, and are being investigated for use in energy transduction and organic electronics, all of which have to do with their electrochemistry. Studies of them have contributed to understanding not only their applications, but also certain aspects of physical organic chemistry in general such as the intersection between hydrogen bonding and electron transfer, the square scheme theoretical concept, and proton coupled electron transfer (PCET). Quinones are extremely reactive in their fully reduced form to the point that they will undergo SN2 reactions with supposedly stable solvents such as dichloromethane in the absence of water to H-bond with and stabilize the quinone dianions. This stabilization is both kinetic and thermodynamic in nature—meaning that it comes both from water molecules drawing electron density away from the quinones through their hydrogen bonds, thus making the SN2 reaction less favorable, and from the water molecules themselves getting in between the quinone dianion and its electrophilic target, thus making the reaction slower due to sterics. Cyclic voltammograms (CV's) of quinones never undergo a set of two ideal redox waves because the presence of oxygenated, protonated functional groups on the surface of the analytical electrodes used to study them causes them to form hydrogen bonds and undergo proton transfer at the surface even if there is nothing in solution that can be a hydrogen bonding or proton transfer guest. The only apparent way to get rid of these groups is a long process of ablation with high temperature hydrogen atoms. The presence of water in solution appears to enhance the non-ideality. In order to accurately describe the quinone-phenol system, which is the subject of a great deal of the active research in this area, it is necessary to consider hydrogen bonding, electron transfer, and proton transfer reactions, not just two of the three. This is easy to do using the “wedge” scheme, which is a theoretical model to organize mechanisms that require three types of reactions done over and over again. Wedge schemes are shown to give good qualitative approximations of the CV’s of duroquinone and 2-naphthol, with the best including proton transfer from a naphthol-naphthol hydrogen bonded complex that forms slowly in solution.
Subjects/Keywords: Analytical chemistry
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APA (6th Edition):
Staley, P. A. (2017). The Electrochemistry of Quinones in Aprotic Solvents. (Thesis). University of California, San Diego. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=10249059
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Staley, Patrick Andrew. “The Electrochemistry of Quinones in Aprotic Solvents.” 2017. Thesis, University of California, San Diego. Accessed February 16, 2019.
http://pqdtopen.proquest.com/#viewpdf?dispub=10249059.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Staley, Patrick Andrew. “The Electrochemistry of Quinones in Aprotic Solvents.” 2017. Web. 16 Feb 2019.
Vancouver:
Staley PA. The Electrochemistry of Quinones in Aprotic Solvents. [Internet] [Thesis]. University of California, San Diego; 2017. [cited 2019 Feb 16].
Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=10249059.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Staley PA. The Electrochemistry of Quinones in Aprotic Solvents. [Thesis]. University of California, San Diego; 2017. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=10249059
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
The University of Arizona
6.
Head, Jeffrey Lee Raynor.
Synthesis and characterization of CuxS nanoparticles for solar cell application.
Degree: 2009, The University of Arizona
URL: http://pqdtopen.proquest.com/#viewpdf?dispub=1464084
► Chalcocite, Cu2S, and djurleite, Cu1.94-1.96 S, copper sulfide nanoparticles have been synthesized. They have been characterized using a multitude of methods. Using x-ray photoelectron…
(more)
▼ Chalcocite, Cu2S, and djurleite, Cu1.94-1.96 S, copper sulfide nanoparticles have been synthesized. They have been characterized using a multitude of methods. Using x-ray photoelectron spectroscopy (XPS), the binding energies of the core electrons have been measured for each type of nanoparticle. Using powder x-ray diffraction and ultraviolet-visible spectroscopy, the crystal structures have been determined and it has been found that the chalcocite nanoparticles have indirect band gap transitions, whereas the djurleite nanoparticles have direct band gap transitions. Using electron microscopy it has been found that the nanoparticles are both single crystalline with size distributions that vary between 8 and 20 nm for the djurleite nanoparticles, and between 10 and 200 nm for the chalcocite nanoparticles. The djurleite nanoparticles have been incorporated into a solar cell device in combination with C60 and using ultraviolet photoelectron spectroscopy (UPS), the band structure of the nanoparticles has been evaluated.
Subjects/Keywords: Chemistry; Analytical
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APA (6th Edition):
Head, J. L. R. (2009). Synthesis and characterization of CuxS nanoparticles for solar cell application. (Thesis). The University of Arizona. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=1464084
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Head, Jeffrey Lee Raynor. “Synthesis and characterization of CuxS nanoparticles for solar cell application.” 2009. Thesis, The University of Arizona. Accessed February 16, 2019.
http://pqdtopen.proquest.com/#viewpdf?dispub=1464084.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Head, Jeffrey Lee Raynor. “Synthesis and characterization of CuxS nanoparticles for solar cell application.” 2009. Web. 16 Feb 2019.
Vancouver:
Head JLR. Synthesis and characterization of CuxS nanoparticles for solar cell application. [Internet] [Thesis]. The University of Arizona; 2009. [cited 2019 Feb 16].
Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=1464084.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Head JLR. Synthesis and characterization of CuxS nanoparticles for solar cell application. [Thesis]. The University of Arizona; 2009. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=1464084
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Cincinnati
7.
Chan, Qilin.
Developing Innovative Metallomics Approaches to Characterize Trace Biometals.
Degree: 2011, University of Cincinnati
URL: http://pqdtopen.proquest.com/#viewpdf?dispub=3450616
► Many elements have been claimed as essential for health but their precise roles in life are largely uncharacterized. The increasing interests in the metals/metalloids…
(more)
▼ Many elements have been claimed as essential for health but their precise roles in life are largely uncharacterized. The increasing interests in the metals/metalloids within biological systems have been driving the fast growth of metallomics, a subject originated to establish the links between metals/metalloids and life. The foundation of metallomics relies on precise determination of biological metal(loid) species. The aim of this dissertation is characterization of such species, primarily metalloproteins, in various biological systems using innovative metallomics approaches. These include several multi-technique methods in which the traditional elemental speciation with HPLC-ICPMS (inductively coupled plasma mass spectrometry) and LC-MS/MS based proteomics were combined to meet the challenges of metalloprotein characterization in animals and plants. Two biologically important elements were intensively studied, zinc (Zn) in murine macrophages and selenium (Se) in plants. The metallomics study in macrophages revealed that Zn played a fundamental role in host defense against <i> Histoplasma capsulatum</i> infection, and a few Zn-binding proteins selectively responded to macrophage activation or the pathogen infection. The Se study elucidated the complete profiles of Se metabolites in kale (<i> Brassica oleracea</i>) and soybean (<i>Glycine max</i>) and more importantly, it discovered the first Se-containing protein in plants, the Se-containing Bowman-Birk proteinase isoinhibitor D-II [<i>Glycine max</i>] derived from the Se-enriched soybean. These findings not only opened new avenues to study Zn’s precise roles in immunity and Se-containing proteins in plants, but also lead the interactions between metallomics and other research areas, such as immunology and proteomics.
Subjects/Keywords: Chemistry; Analytical
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APA (6th Edition):
Chan, Q. (2011). Developing Innovative Metallomics Approaches to Characterize Trace Biometals. (Thesis). University of Cincinnati. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=3450616
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Chan, Qilin. “Developing Innovative Metallomics Approaches to Characterize Trace Biometals.” 2011. Thesis, University of Cincinnati. Accessed February 16, 2019.
http://pqdtopen.proquest.com/#viewpdf?dispub=3450616.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Chan, Qilin. “Developing Innovative Metallomics Approaches to Characterize Trace Biometals.” 2011. Web. 16 Feb 2019.
Vancouver:
Chan Q. Developing Innovative Metallomics Approaches to Characterize Trace Biometals. [Internet] [Thesis]. University of Cincinnati; 2011. [cited 2019 Feb 16].
Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3450616.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Chan Q. Developing Innovative Metallomics Approaches to Characterize Trace Biometals. [Thesis]. University of Cincinnati; 2011. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3450616
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Arkansas
8.
Aggarwal, Anupama.
Studies toward the development of a microelectrode array for detection of dopamine through redox cycling.
Degree: 2011, University of Arkansas
URL: http://pqdtopen.proquest.com/#viewpdf?dispub=3473445
► Redox cycling is an electrochemical technique that cycles the reversible redox species between its oxidative states repeatedly on generator and collector electrodes. Two or…
(more)
▼ Redox cycling is an electrochemical technique that cycles the reversible redox species between its oxidative states repeatedly on generator and collector electrodes. Two or more individually-addressable microelectrodes located close to each other allow redox cycling to be possible. Electrochemical behavior of a biologically important molecule, dopamine is examined under redox cycling conditions. To our knowledge, this is the first report on detection of physiological concentration of dopamine in presence of up to 100 times excess ascorbate with the use of redox cycling, without the involvement of polymer coating such as Nafion<sup>®</sup>. Microfabrication was used to produce different geometries (parallel bands and concentric rings) with feature size of 4 μm or 25 μm and inter-electrode spacing of 4 μm or 25 μm on a single substrate (microelectrode arrays). Comparison of the individual electrochemical response of different arrangements of individually addressable elements composing generator and collector electrodes on band and ring microelectrode arrays is presented. The individually addressable nature of microelectrodes allowed the study of different combinations of anodic and cathodic electrodes and the current at the individual elements composing the generator and the collector. Reversible electroactive species hexaammineruthenium (III) chloride in a 0.5 M potassium chloride electrolyte solution was used to perform the electrochemical characterization and comparisons of the microelectrode arrays. Behavior of band and ring microelectrodes in an array format during redox cycling is compared to each other as well as to the available theory. The optimized geometry and arrangement of microelectrodes is used to exhibit detection of dopamine with and without redox cycling. Comparison of dopamine behavior in presence and absence of redox cycling and other available methods of detection is provided. Detection of dopamine in presence of some of the common interferences (ascorbate and 3,4-dihydroxyphenylacetic acid) is shown with and without redox cycling on unmodified electrodes as well as on electrodes modified with Nafion<sup>®</sup>. These studies indicate that redox cycling can detect dopamine at physiologically relevant concentrations in presence of interferences, but will require the coating of electrodes with Nafion in addition to broaden the range of interfering compounds that can be eliminated. Traditional voltammetric methods to detect dopamine cannot measure resting, or static dopamine concentrations where redox cycling would be of further interest. Redox cycling provides elimination from the interfering ascorbate signal, enhancing the sensitivity toward the analyte simultaneously. Based on the knowledge gathered from comparison of different electrodes and redox cycling of dopamine, an interdigitated microelectrode probe design suitable for investigation of redox cycling of dopamine is fabricated. The probe contains array having dimensions…
Subjects/Keywords: Chemistry; Analytical
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APA (6th Edition):
Aggarwal, A. (2011). Studies toward the development of a microelectrode array for detection of dopamine through redox cycling. (Thesis). University of Arkansas. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=3473445
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Aggarwal, Anupama. “Studies toward the development of a microelectrode array for detection of dopamine through redox cycling.” 2011. Thesis, University of Arkansas. Accessed February 16, 2019.
http://pqdtopen.proquest.com/#viewpdf?dispub=3473445.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Aggarwal, Anupama. “Studies toward the development of a microelectrode array for detection of dopamine through redox cycling.” 2011. Web. 16 Feb 2019.
Vancouver:
Aggarwal A. Studies toward the development of a microelectrode array for detection of dopamine through redox cycling. [Internet] [Thesis]. University of Arkansas; 2011. [cited 2019 Feb 16].
Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3473445.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Aggarwal A. Studies toward the development of a microelectrode array for detection of dopamine through redox cycling. [Thesis]. University of Arkansas; 2011. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3473445
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
9.
Freudenthal, John.
Mueller Matrix Imaging in Crystallography| The Chiroptics of Pattern Formation.
Degree: 2012, New York University
URL: http://pqdtopen.proquest.com/#viewpdf?dispub=3502694
► The interactions of light and anisotropic matter are described with a specific focus on multilayered structures and bianisotropic (meso-chiral) materials. These interactions can be…
(more)
▼ The interactions of light and anisotropic matter are described with a specific focus on multilayered structures and bianisotropic (meso-chiral) materials. These interactions can be described by the 4x4 Mueller matrix that captures all of the linear optical properties of a sample, LB, CB, LD, CD, and depolarization due to scattering from a single set of measurements. Imaging polarimetry is particularly informative for heterogeneous substances. Among the systems analyzed are the following: mixed crystals of sodium chlorate and bromate, rhythmic precipitation of phthalic acid, simple spherulites of sorbitol, helical crystals of hippuric acid, and laminar high polymers. Our analyses inform the structure of complex polycrystalline materials such as the patterned growth of polycrystalline ensembles, and analyze the chiroptics induced by highly linearly anisotropic materials.
Subjects/Keywords: Chemistry; Analytical
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APA (6th Edition):
Freudenthal, J. (2012). Mueller Matrix Imaging in Crystallography| The Chiroptics of Pattern Formation. (Thesis). New York University. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=3502694
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Freudenthal, John. “Mueller Matrix Imaging in Crystallography| The Chiroptics of Pattern Formation.” 2012. Thesis, New York University. Accessed February 16, 2019.
http://pqdtopen.proquest.com/#viewpdf?dispub=3502694.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Freudenthal, John. “Mueller Matrix Imaging in Crystallography| The Chiroptics of Pattern Formation.” 2012. Web. 16 Feb 2019.
Vancouver:
Freudenthal J. Mueller Matrix Imaging in Crystallography| The Chiroptics of Pattern Formation. [Internet] [Thesis]. New York University; 2012. [cited 2019 Feb 16].
Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3502694.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Freudenthal J. Mueller Matrix Imaging in Crystallography| The Chiroptics of Pattern Formation. [Thesis]. New York University; 2012. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3502694
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
The George Washington University
10.
Brennan, Ryan Garrett.
Challenges in plasma spectrometry for the analysis of limited, expensive, and toxic materials| From aerosol generation, diagnostics, to practical applications.
Degree: 2009, The George Washington University
URL: http://pqdtopen.proquest.com/#viewpdf?dispub=3344702
► The objective of this research is centered on fundamental studies to improve the analytical performance of plasma based elemental mass spectrometry and optical emission…
(more)
▼ The objective of this research is centered on fundamental studies to improve the analytical performance of plasma based elemental mass spectrometry and optical emission spectrometry with an emphasis on the development of liquid sample introduction devices suitable for the analysis of limited, expensive, and toxic materials. The use and improvement of the demountable-direct injection high efficiency nebulizer (d-DIHEN) is investigated as a suitable interface for high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS) to detect toxic arsenic species at nano-liter solution flow rates and sample volume. Separation of five arsenic species is achieved in less than 12 minutes at a solution flow rate of 0.9 μL min-1 using a 50 nL sample injection. Precision values for peak height and area of five arsenic species range from 0.5% to 6.5% RSD and absolute detection limits are within 0.4 pg to 5.4 pg arsenic, which are comparable to previously reported data at higher solution uptake rates (20 μL min-1 to 1 mL min-1 ) and larger sample injection volumes (20 μL to 100 μL). To increase the potential for the d-DIHEN to be used more widely, an automated sample introduction system is successfully incorporated for the first time, making the d-DIHEN a universal direct injection micronebulizer. The measurement of the phosphorus content in acid-digested nucleotides and deoxyribonucleic acid (DNA) is performed with an inductively coupled plasma optical emission spectrometer (ICP-OES). With this experimental setup, the solution uptake rate and volume are reduced from 170 μL min-1 to 30 μL min-1 and 10 mL to 2.4 mL, respectively, thereby reducing the required DNA sample mass for solutions containing 3 μg g -1 P, from 300 μg to 72 μg DNA, in comparison to previous analyses. The use of direct injection also improves P (I) 213.617 nm sensitivity by a factor of 4 on average. The d-DIHEN with high performance-ICP-OES methodology allows for the quantification of DNA mass at P mass fractions as low as 0.5 μg g-1, further reducing the required DNA mass to 12 μg, with small uncertainty (≤ 0.4%). This successful approach will aide in the development and certification of nucleic acid certified reference materials (CRMs), particularly for these samples that are typically limited in volume. The measurement of key aerosol parameters is essential for the development of an efficient low-flow nebulizer. Interferometric Droplet Imaging (IDI) with the combination of particle tracking velocimetry (PTV), is investigated to provide spatial mapping of surviving droplets in an ICP while simultaneously determining droplet size, velocity, and evaporation rate. Key findings include: (1) droplets traveling at velocities of 15 ms-1 and higher are more likely to survive the high temperature plasma, causing local cooling effects, (2) surviving droplets have a size…
Subjects/Keywords: Chemistry; Analytical
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APA ·
Chicago ·
MLA ·
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CSE |
Export
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APA (6th Edition):
Brennan, R. G. (2009). Challenges in plasma spectrometry for the analysis of limited, expensive, and toxic materials| From aerosol generation, diagnostics, to practical applications. (Thesis). The George Washington University. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=3344702
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Brennan, Ryan Garrett. “Challenges in plasma spectrometry for the analysis of limited, expensive, and toxic materials| From aerosol generation, diagnostics, to practical applications.” 2009. Thesis, The George Washington University. Accessed February 16, 2019.
http://pqdtopen.proquest.com/#viewpdf?dispub=3344702.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Brennan, Ryan Garrett. “Challenges in plasma spectrometry for the analysis of limited, expensive, and toxic materials| From aerosol generation, diagnostics, to practical applications.” 2009. Web. 16 Feb 2019.
Vancouver:
Brennan RG. Challenges in plasma spectrometry for the analysis of limited, expensive, and toxic materials| From aerosol generation, diagnostics, to practical applications. [Internet] [Thesis]. The George Washington University; 2009. [cited 2019 Feb 16].
Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3344702.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Brennan RG. Challenges in plasma spectrometry for the analysis of limited, expensive, and toxic materials| From aerosol generation, diagnostics, to practical applications. [Thesis]. The George Washington University; 2009. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3344702
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Indiana University
11.
Kurulugama, Ruwan T.
Overtone mobility spectrometry.
Degree: 2009, Indiana University
URL: http://pqdtopen.proquest.com/#viewpdf?dispub=3344776
► Mobility of ions through a static buffer gas as a means of separating components in complex mixtures was introduced in 1970 by Cohen and…
(more)
▼ Mobility of ions through a static buffer gas as a means of separating components in complex mixtures was introduced in 1970 by Cohen and Karasek and later named as ion mobility spectrometry (IMS). Currently, there are two other methods that are based on this method; differential mobility analysis (DMA), in which ions migrate through a flowing gas under the influence of a field, and, field asymmetric ion mobility spectrometry (FAIMS) which separates ions based on differences between high- and low-field mobilities. IMS and its variations are extensively used in various applications as a means of characterizing a range of analytes as well as simplifying the analysis of complex mixtures through the removal of chemical noise. The work presented here introduces a new separation technique called overtone mobility spectrometry (OMS) based on mobilities of ions in gas phase. OMS approach uses multiple segmented drift regions with modulated drift fields to produce conditions that allow only ions with appropriate mobilities to pass through the instrument. Therefore, the instrument acts as a mobility-filter for continuous ion sources. By changing the frequency of the drift field application, it is possible to tune this instrument to transmit ions having different mobilities. Also, a scan over a wide range of field application frequencies for a single ion species shows a peak corresponding to the expected resonance time of the ions in one drift segment as well as a series of peaks at higher overtone frequencies. The measured resolving power increases for higher overtones making it possible to resolve structures that were unresolved in the region of the fundamental frequency. Because of the ability to select ions in different frequency regions, including those that are associated with higher overtones, we refer to the approach as <i>Overtone Mobility Spectrometry</i> (OMS). The majority of the work presented here involves the development of this technique and its characterization using different systems such as simple isobaric carbohydrate mixtures and complex protein digests. Also presented here is the theoretical evaluation of the resolving power and ion simulation data to understand the basis of the peak formation in the frequency domain.
Subjects/Keywords: Chemistry; Analytical
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APA ·
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APA (6th Edition):
Kurulugama, R. T. (2009). Overtone mobility spectrometry. (Thesis). Indiana University. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=3344776
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Kurulugama, Ruwan T. “Overtone mobility spectrometry.” 2009. Thesis, Indiana University. Accessed February 16, 2019.
http://pqdtopen.proquest.com/#viewpdf?dispub=3344776.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Kurulugama, Ruwan T. “Overtone mobility spectrometry.” 2009. Web. 16 Feb 2019.
Vancouver:
Kurulugama RT. Overtone mobility spectrometry. [Internet] [Thesis]. Indiana University; 2009. [cited 2019 Feb 16].
Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3344776.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Kurulugama RT. Overtone mobility spectrometry. [Thesis]. Indiana University; 2009. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3344776
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
The University of Arizona
12.
Yoo, Heemin.
Interfacial structure and dynamics of nematic 4-n-pentyl-4'-cyanobiphenyl liquid crystals on silver, silica and modified silica substrates.
Degree: 2009, The University of Arizona
URL: http://pqdtopen.proquest.com/#viewpdf?dispub=3359811
► The process of forcibly dewetting a solid substrate from a bulk liquid so as to leave a thin residual layer on the surface is…
(more)
▼ The process of forcibly dewetting a solid substrate from a bulk liquid so as to leave a thin residual layer on the surface is referred to as forced dewetting. This novel experimental approach helps to investigate interfacial species by minimizing the interference of the bulk liquid when coupled with spectroscopy. In this work, the scope of liquids investigating using this approach has been expanded from simple fluids to one type of complex fluid, a nematic liquid crystal, 4-n-pentyl-4’-cyanobiphenyl (5CB). In order to better understand the interfacial behavior of the simple fluids, water, chloroform, and n-pentane vapors were adsorbed onto ω-terminated SAM-modifed Ag (11-mercaptoundecanoic acid, 11-mercaptoundenanol, and undecanethiol) surfaces under vapor-saturated conditions. The kinetics of solvent adsorption on each of these surfaces were investigated and the thicknesses of the adsorbed layer were compared to predictions from Lifshitz theory of long-range van der Waals interactions. Although the predicted thicknesses do not match the experimental values for adsorbed films, the predicted thicknesses do match those observed experimentally using forced dewetting. The correlation between these predicted and observed thicknesses implies that residual film formation under the conditions of forced dewetting used in this laboratory is not appropriately described by fluid dynamic models as previously thought. Instead, residual film formation appears to be dictated by interfacial forces alone. The surface adsorption behavior of 5CB was investigated using surface-enhanced Raman spectroscopy with the aid of localized surface plasmon resonances-surface plasmon polaritron coupling. The average orientation of the thin 5CB layer (∼1 nm) was 20 evaluated using SERS surface selection rules. The results clearly indicate that 5CB is adsorbed to smooth Ag surface in a facial orientation with π-d orbital interaction suggested. Finally, forced dewetting studies of bare, —NH2-terminated SAM, and —CH3-terminated SAM modified-SiO2 substrates from 5CB were undertaken. Residual layer thicknesses were monitored as a function of substrate velocity (1–1726 μm/s). The transition from the regime in which interfacial forces dictate residual layer thickness to the regime in which fluid dynamic forces dictate thickness was observed for the first time and was evaluated in terms of the average 5CB director orientation. Unlike simple fluids, 5CB has strong interfacial interactions from surface anchoring depending on the chemical nature of the substrate, which makes the residual layer thicknesses at least 100 times larger than observed in simple fluids. These results are important in understanding the coherence length over which surface anchoring is important as a function of surface free energy.
Subjects/Keywords: Chemistry; Analytical
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APA ·
Chicago ·
MLA ·
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CSE |
Export
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APA (6th Edition):
Yoo, H. (2009). Interfacial structure and dynamics of nematic 4-n-pentyl-4'-cyanobiphenyl liquid crystals on silver, silica and modified silica substrates. (Thesis). The University of Arizona. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=3359811
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Yoo, Heemin. “Interfacial structure and dynamics of nematic 4-n-pentyl-4'-cyanobiphenyl liquid crystals on silver, silica and modified silica substrates.” 2009. Thesis, The University of Arizona. Accessed February 16, 2019.
http://pqdtopen.proquest.com/#viewpdf?dispub=3359811.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Yoo, Heemin. “Interfacial structure and dynamics of nematic 4-n-pentyl-4'-cyanobiphenyl liquid crystals on silver, silica and modified silica substrates.” 2009. Web. 16 Feb 2019.
Vancouver:
Yoo H. Interfacial structure and dynamics of nematic 4-n-pentyl-4'-cyanobiphenyl liquid crystals on silver, silica and modified silica substrates. [Internet] [Thesis]. The University of Arizona; 2009. [cited 2019 Feb 16].
Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3359811.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Yoo H. Interfacial structure and dynamics of nematic 4-n-pentyl-4'-cyanobiphenyl liquid crystals on silver, silica and modified silica substrates. [Thesis]. The University of Arizona; 2009. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3359811
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
13.
Aernecke, Matthew J.
Microsphere array based vapor sensing| New capabilities and applications.
Degree: 2009, Tufts University
URL: http://pqdtopen.proquest.com/#viewpdf?dispub=3378987
► The work presented in this thesis details new capabilities and applications of a fluorescence-based microsphere array vapor sensing system developed in the Walt Laboratory…
(more)
▼ The work presented in this thesis details new capabilities and applications of a fluorescence-based microsphere array vapor sensing system developed in the Walt Laboratory over a period of several years. The system is designed on the principles of mammalian olfaction in that several cross-reactive and non-specific sensors are integrated into a high density array format. The system responds to an analyte vapor in a cross-reactive manner generating a multi-dimensional signal that can be used to train a pattern recognition program to identify subsequent exposures of the array to learned vapors. Several ways in which the classification accuracy of this system can be improved are explored. New sensors based on porphyrin compounds were developed and their vapor sensing properties were characterized. This new class of sensors was systematically evaluated within the context of existing sensor types to determine their effectiveness in expanding the range of chemical compounds that can be reliably detected and classified. A second route to improving the classification accuracy of the system involves implementing a biomimetic data processing scheme. This method emulates the biological process of active sampling, or 'sniffing', and has been shown to reduce the number of errors in classification when the system is challenged with a broad range of chemical species. Finally, the ability to monitor the entire fluorescence spectrum from many individual sensors offers the opportunity to optimize the discrete wavelengths at which a response is observed. A method to collect this spectrum in a highly parallel fashion with excellent temporal resolution has been developed and is shown to improve the classification accuracy when information from multiple wavelengths is incorporated into a response matrix. An exploration into new applications of this system is presented. Specifically, a preliminary investigation into using this device during the early stages of a fire investigation is described. The results of this investigation show that the system is capable of correctly detecting and classifying three types of ignitable liquids when presented in a realistic sample matrix. The design and construction of a portable device is also described and the results of field experiments are presented. The results of these studies demonstrate the basic functionality of the portable device and establish its usefulness in the detection of petroleum distillates in the ambient environment.
Subjects/Keywords: Chemistry; Analytical
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APA ·
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MLA ·
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CSE |
Export
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APA (6th Edition):
Aernecke, M. J. (2009). Microsphere array based vapor sensing| New capabilities and applications. (Thesis). Tufts University. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=3378987
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Aernecke, Matthew J. “Microsphere array based vapor sensing| New capabilities and applications.” 2009. Thesis, Tufts University. Accessed February 16, 2019.
http://pqdtopen.proquest.com/#viewpdf?dispub=3378987.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Aernecke, Matthew J. “Microsphere array based vapor sensing| New capabilities and applications.” 2009. Web. 16 Feb 2019.
Vancouver:
Aernecke MJ. Microsphere array based vapor sensing| New capabilities and applications. [Internet] [Thesis]. Tufts University; 2009. [cited 2019 Feb 16].
Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3378987.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Aernecke MJ. Microsphere array based vapor sensing| New capabilities and applications. [Thesis]. Tufts University; 2009. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3378987
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
The George Washington University
14.
Shrestha, Bindesh.
Single cell analysis and tissue imaging by laser ablation and mass spectrometry.
Degree: 2010, The George Washington University
URL: http://pqdtopen.proquest.com/#viewpdf?dispub=3389741
► The direct analysis of biochemicals in tissues and single cells is critical for understanding living organisms. Due to excellent selectivity and sensitivity, mass spectrometry…
(more)
▼ The direct analysis of biochemicals in tissues and single cells is critical for understanding living organisms. Due to excellent selectivity and sensitivity, mass spectrometry (MS) has proven to be an invaluable tool for the analysis of the biomolecules. Recent developments in atmospheric pressure direct ionization sources have enabled the in situ analysis of biological samples without external influences (e.g., purification, extraction, matrix addition etc.) that might alter their biochemical makeup. The work presented in this dissertation shows my efforts to utilize two novel atmospheric pressure (AP) direct ionization methodologies, AP infrared (IR) matrix-assisted laser desorption ionization (MALDI) and laser ablation electrospray ionization (LAESI) MS, for metabolomics, tissue imaging and single cell analysis. Chapter 1 introduces analytical techniques used for the analysis of tissues and single cells. The fundamental aspects of IR laser ablation and its utilization in two direct ionization techniques, AP IR-MALDI and LAESI, are reviewed. Chapter 2 introduces AP IR-MALDI for MS. It presents proof-of-principle molecular imaging of mock peptide samples at atmospheric pressure. The utility of AP IR-MALDI for plant tissue imaging and metabolomics are also discussed. Chapter 3 describes the AP IR-MALDI analysis of various pharmaceuticals directly in their commercial formulations, as well as endogenous metabolites, exogenous drug metabolites and synthetic polymers in urine. Chapter 4 presents the application of mid-infrared laser ablation for molecular imaging. The dynamics of the ablation plume and ion production in AP IR-MALDI and LAESI are compared. In Chapter 5 metabolites and lipids are identified in mouse brain sections using MS with AP IR-MALDI and LAESI ion production. Reactive LAESI relies on interactions between the laser ablated species and reactants, e.g., Li +, introduced through the electrospray. This new modality of LAESI enables the analysis of samples with otherwise low ion yields. Chapter 6 discusses the metabolic analysis of single cells by MS at atmospheric pressure. This breakthrough is made possible by the tight focusing of mid-IR laser light through an etched optical fiber tip and accurate aiming of cells for ablation through visualization and micromanipulation. Similar to conventional LAESI, the ablated plume is postionized by an electrospray. Chapter 7 surveys the major challenges in the field of atmospheric pressure ion production based on mid-IR laser ablation. The need for the analysis of smaller cells, reactive LAESI-MS, ultrahigh resolution LAESI-MS, and the potential application of LAESI-MS in laser surgery are discussed.
Subjects/Keywords: Chemistry; Analytical
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Shrestha, B. (2010). Single cell analysis and tissue imaging by laser ablation and mass spectrometry. (Thesis). The George Washington University. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=3389741
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Shrestha, Bindesh. “Single cell analysis and tissue imaging by laser ablation and mass spectrometry.” 2010. Thesis, The George Washington University. Accessed February 16, 2019.
http://pqdtopen.proquest.com/#viewpdf?dispub=3389741.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Shrestha, Bindesh. “Single cell analysis and tissue imaging by laser ablation and mass spectrometry.” 2010. Web. 16 Feb 2019.
Vancouver:
Shrestha B. Single cell analysis and tissue imaging by laser ablation and mass spectrometry. [Internet] [Thesis]. The George Washington University; 2010. [cited 2019 Feb 16].
Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3389741.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Shrestha B. Single cell analysis and tissue imaging by laser ablation and mass spectrometry. [Thesis]. The George Washington University; 2010. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3389741
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
15.
Braun, Gary Bernard.
General approach for tagged SERS-active nanomaterials.
Degree: 2010, University of California, Santa Barbara
URL: http://pqdtopen.proquest.com/#viewpdf?dispub=3398838
► Over the past decade the emphasis on single-molecule sensitivity of surface-enhanced Raman spectroscopy (SERS) has brought to prominence the special role played by so-called…
(more)
▼ Over the past decade the emphasis on single-molecule sensitivity of surface-enhanced Raman spectroscopy (SERS) has brought to prominence the special role played by so-called SERS hot spots, oftentimes nanometer-scale junctions between nanoparticles (NPs). In this work, molecular linkers are used to mediate the assembly of NPs to dimers and small clusters. When the SERS enhancement is optimized, the aggregation process is quenched by polymer and protein stabilizers that subsequently act as encapsulants resulting in SERS substrates with unprecedented enhancement uniformity, reproducibility, and stability. The polymer-stabilized NP junctions were then imprinted with a variety of small molecules that permeated the polymer coat and displaced the linker from the hot spot. The surface adsorption process can be monitored in real time with spectral analysis aiding optimization. The average SERS enhancement of these SERS “nanocapsules” was found to be at least 300× greater than for single NPs. Single-particle statistics showed that almost every nanocapsule produced intense SERS, suggesting that they are NP dimers and small clusters with the probe molecule resident in a hot spot. Nanocapsules were functionalized and shown to compete successfully with fluorescence imaging in multiplexed identification of cancer cell epitopes at the single-cell and single-nanotag level.
Subjects/Keywords: Chemistry; Analytical
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Braun, G. B. (2010). General approach for tagged SERS-active nanomaterials. (Thesis). University of California, Santa Barbara. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=3398838
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Braun, Gary Bernard. “General approach for tagged SERS-active nanomaterials.” 2010. Thesis, University of California, Santa Barbara. Accessed February 16, 2019.
http://pqdtopen.proquest.com/#viewpdf?dispub=3398838.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Braun, Gary Bernard. “General approach for tagged SERS-active nanomaterials.” 2010. Web. 16 Feb 2019.
Vancouver:
Braun GB. General approach for tagged SERS-active nanomaterials. [Internet] [Thesis]. University of California, Santa Barbara; 2010. [cited 2019 Feb 16].
Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3398838.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Braun GB. General approach for tagged SERS-active nanomaterials. [Thesis]. University of California, Santa Barbara; 2010. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3398838
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Purdue University
16.
Habicht, Steven C.
Gas-phase ion-molecule reactions and laser-induced acoustic desorption mass spectrometry for complex mixture analysis and structural elucidation.
Degree: 2010, Purdue University
URL: http://pqdtopen.proquest.com/#viewpdf?dispub=3402442
► Mass spectrometry (MS) has proven invaluable in the field of mixture analysis and structural elucidation of mixture components, due to its high sensitivity, selectivity…
(more)
▼ Mass spectrometry (MS) has proven invaluable in the field of mixture analysis and structural elucidation of mixture components, due to its high sensitivity, selectivity and speed. Tandem mass spectrometry (MS/MS) utilizing collision-activated dissociation (CAD) has become the technique of choice for structural elucidation of unknown analytes. The coupling of MS with high performance liquid chromatography (HPLC) has allowed the trace level analysis of components in very complex mixtures. However, these experiments alone do not always allow for unambiguous identification of an unknown analyte in a mixture. The experiments described in this thesis were aimed at providing more detailed structural information of mixture components that is difficult to obtain by established methods. In the first section (chapters 3, 4 and 5), gas-phase ion-molecule reactions were developed and implemented on a commercially available linear quadrupole ion trap (LQIT) mass spectrometer (Finnigan LTQ) to make these methods more widely applicable to the pharmaceutical industry. First, the performance a reagent mixing manifold that allows ion-molecule reactions to be carried out in the LQIT was probed by examining an established ion-molecule reaction previously employed on a Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer. Next, a novel ion-molecule reaction was developed using the new setup that exploits the favored formation of sodium and ammonium ion adducts when oxygen-containing analytes are ionized via electrospray ionization (ESI). Finally, the modified instrument was coupled with an HPLC to demonstrate that LC-MS3 using ion-molecule reactions and collision-activated dissociation (CAD) provides a powerful technique for the analysis of low level drug metabolites and impurities in pharmaceutical mixtures. Further, these methods were automated using the data-dependent features of the instrument, allowing for detailed structural information of unknown analytes “on-the-fly.” The second section (chapters 6, 7 and 8) focus on the development of laser-induced acoustic desorption (LIAD) methodology for structural elucidation. First, the coupling of this technique with a LQIT is presented and LIAD experiments are compared to those performed in a FT-ICR. Next, the use of LIAD in studies on the gas-phase reactivity of phenyl radicals toward tetrapeptides is presented. Very interesting results were obtained on the susceptibility of the disulfide bond to radical attack, especially considering the important role these bonds play in defining proteins’ structures. Finally, the ability of LIAD-based methods to perform unparalleled characterization of petroleum fractions is presented. Also, the development of ClMn(H2O)+ chemical ionization (CI) on the LQIT is examined. The coupling of this CI method with LIAD on the LQIT is the initial step for future implementation of this methodology on a hydrid LQIT/FT-ICR instrument to provide one of the most powerful…
Subjects/Keywords: Chemistry; Analytical
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APA (6th Edition):
Habicht, S. C. (2010). Gas-phase ion-molecule reactions and laser-induced acoustic desorption mass spectrometry for complex mixture analysis and structural elucidation. (Thesis). Purdue University. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=3402442
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Habicht, Steven C. “Gas-phase ion-molecule reactions and laser-induced acoustic desorption mass spectrometry for complex mixture analysis and structural elucidation.” 2010. Thesis, Purdue University. Accessed February 16, 2019.
http://pqdtopen.proquest.com/#viewpdf?dispub=3402442.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Habicht, Steven C. “Gas-phase ion-molecule reactions and laser-induced acoustic desorption mass spectrometry for complex mixture analysis and structural elucidation.” 2010. Web. 16 Feb 2019.
Vancouver:
Habicht SC. Gas-phase ion-molecule reactions and laser-induced acoustic desorption mass spectrometry for complex mixture analysis and structural elucidation. [Internet] [Thesis]. Purdue University; 2010. [cited 2019 Feb 16].
Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3402442.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Habicht SC. Gas-phase ion-molecule reactions and laser-induced acoustic desorption mass spectrometry for complex mixture analysis and structural elucidation. [Thesis]. Purdue University; 2010. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3402442
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
17.
Jelinek, Christine A.
Characterizing the post-translational modifications of human serum albumin as they correlate to cardiac ischemia.
Degree: 2012, The Johns Hopkins University
URL: http://pqdtopen.proquest.com/#viewpdf?dispub=3528552
► During cardiac ischemia, human serum albumin's tertiary structure is altered by a yet unknown mechanism within minutes of arterial occlusion. Herein it is shown…
(more)
▼ During cardiac ischemia, human serum albumin's tertiary structure is altered by a yet unknown mechanism within minutes of arterial occlusion. Herein it is shown that ischemic conditions induce changes to albumin's post-translational protein profile that can be quantitatively assayed using mass spectrometry. Profiling albumin post-translational modifications in a model cohort is suggestive of the diagnostic power of an albumin biomarker panel as an ischemic indicator. Specifically, ischemic conditions lead to altered expression of three first domain PTMs on residues Cys34, Ser58, and Cys91 respectively. The focus of this research has been the use of targeted mass spectrometry approaches to examine the tertiary protein features of albumin in both qualitative and a quantitative contexts. A number of albumin amino acid residues had previously been identified as sites of albumin post-translational modification. However, studies to characterize and profile the human serum albumin protein had previously been limited both in depth and in scope. To determine if preceding efforts had sufficiently and fully characterized the protein, we multiplexed proteomic technologies during characterization efforts. In so doing, we established a profile of endogenous albumin PTMs from which to determine both basal and disease-altered stoichiometry. To correlate change in albumin's modification expression to specific clinical states, we converted profiling efforts into an assay format using selected reaction monitoring mass spectrometry. In this, we have avoided more canonical techniques or protocols during protein characterization in favor of instrumentation and methods that provide increased speed, throughput, and/or reproducibility during analysis. To this end, we have replaced manual sample preparation steps with an automated system directly interfaced to the mass spectrometer. Integrating a comprehensive stand-alone proteomic workstation into the quantitative mass spectrometric platform expands the overall utility of mass spectrometry with regards to clinical diagnostics. The MS/MS capabilities of the mass spectrometer allow for specific and sensitive measurements in complex biological fluids (like clinical specimens—blood, CSF, urine, etc.). The robotics capabilities of the sample preparation workstation allow for high-throughput, highly reproducible work flows. Automating sample preparation builds quality assessment and measured best practices into mass spectrometric assay design. The end result is a robust, high-throughput pipeline, rigorous in regards to sensitivity of detection, accuracy of measurement, and reproducibility of process.
Subjects/Keywords: Chemistry; Analytical
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Jelinek, C. A. (2012). Characterizing the post-translational modifications of human serum albumin as they correlate to cardiac ischemia. (Thesis). The Johns Hopkins University. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=3528552
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Jelinek, Christine A. “Characterizing the post-translational modifications of human serum albumin as they correlate to cardiac ischemia.” 2012. Thesis, The Johns Hopkins University. Accessed February 16, 2019.
http://pqdtopen.proquest.com/#viewpdf?dispub=3528552.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Jelinek, Christine A. “Characterizing the post-translational modifications of human serum albumin as they correlate to cardiac ischemia.” 2012. Web. 16 Feb 2019.
Vancouver:
Jelinek CA. Characterizing the post-translational modifications of human serum albumin as they correlate to cardiac ischemia. [Internet] [Thesis]. The Johns Hopkins University; 2012. [cited 2019 Feb 16].
Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3528552.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Jelinek CA. Characterizing the post-translational modifications of human serum albumin as they correlate to cardiac ischemia. [Thesis]. The Johns Hopkins University; 2012. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3528552
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
18.
Pope, Daniel Joseph.
Particle matter measurements for inspection/maintenance programs.
Degree: 2010, University of Denver
URL: http://pqdtopen.proquest.com/#viewpdf?dispub=1470795
► Particle emissions from Heavy Duty Diesel Vehicles (HDDVs) are currently measured by opacity or dynamometer gravimetric analysis. The Electronic Tailpipe Particle Sensor (ETaPS) is…
(more)
▼ Particle emissions from Heavy Duty Diesel Vehicles (HDDVs) are currently measured by opacity or dynamometer gravimetric analysis. The Electronic Tailpipe Particle Sensor (ETaPS) is an inexpensive measurement device purported to give real time response to particle mass and was proposed as a possible addition to Inspection/Maintenance (I/M) programs. There were three goals to this study. The first was to verify ETaPS response to particle mass of HDDV exhaust. Integrated ETaPS signal was plotted against filter weight from dynamometer gravimetric analysis and a correlation was found. The second goal was to find a correlation between ETaPS readings and the Remote Sensing Detector (RSD). These tests were invalidated due to interference from power lines greatly affecting the ETaPS signal. The final goal was to find a relationship between the RSD and the dynamometer gravimetric analysis. Comparisons were made from averaged RSD smoke data, and averaged gravimetric data for each HDDV undergoing both tests. A measurable difference was found for RSD smoke readings between Diesel Particle Filter (DPF) equipped vs. non-DPF and DPF bypassed HDDVs.
Subjects/Keywords: Chemistry; Analytical
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APA ·
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Export
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APA (6th Edition):
Pope, D. J. (2010). Particle matter measurements for inspection/maintenance programs. (Thesis). University of Denver. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=1470795
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Pope, Daniel Joseph. “Particle matter measurements for inspection/maintenance programs.” 2010. Thesis, University of Denver. Accessed February 16, 2019.
http://pqdtopen.proquest.com/#viewpdf?dispub=1470795.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Pope, Daniel Joseph. “Particle matter measurements for inspection/maintenance programs.” 2010. Web. 16 Feb 2019.
Vancouver:
Pope DJ. Particle matter measurements for inspection/maintenance programs. [Internet] [Thesis]. University of Denver; 2010. [cited 2019 Feb 16].
Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=1470795.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Pope DJ. Particle matter measurements for inspection/maintenance programs. [Thesis]. University of Denver; 2010. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=1470795
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Pittsburgh
19.
Xiong, Hui.
Development and applications of nanofabricated electrodes for scanning electrochemical microscopy.
Degree: 2008, University of Pittsburgh
URL: http://pqdtopen.proquest.com/#viewpdf?dispub=3300577
► Over the last decade, dramatic progresses in fabrication and synthesis of nanomaterials have enabled reproducible and controlled production of nanometer-sized structures with desired size,…
(more)
▼ Over the last decade, dramatic progresses in fabrication and synthesis of nanomaterials have enabled reproducible and controlled production of nanometer-sized structures with desired size, shape, physical and chemical properties. Nanostructures created in this fashion are essential building blocks of complex nanosystems for various applications. In particular, electronically conductive nanostructures are attractive candidates as electrode materials for both fundamental studies and electrochemical applications in fields such as sensors, energy storage, functional molecular electronic devices, and electrocatalysis. In my Ph.D. work, I explored frontiers in nanoscale electrochemistry utilizing novel electrode systems based on conductive nanostructures with shape and size controlled by advanced nanofabrication/synthesis methods. Specifically, I developed a new methodology based on scanning electrochemical microscopy (SECM) to discover that an individual one-dimensional nanostructure such as a metal nanoband and a single-walled carbon nanotube serves as a highly reactive electrode. This discovery is of great importance for future applications of the novel nanomaterials. Moreover, by integrating modern nanofabrication methods, I created nanometer-sized electrodes with controlled size and geometry. The significance of this achievement is that better spatial resolution will be obtainable by utilizing these nanofabricated electrodes as probes of SECM.
Subjects/Keywords: Chemistry; Analytical
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Xiong, H. (2008). Development and applications of nanofabricated electrodes for scanning electrochemical microscopy. (Thesis). University of Pittsburgh. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=3300577
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Xiong, Hui. “Development and applications of nanofabricated electrodes for scanning electrochemical microscopy.” 2008. Thesis, University of Pittsburgh. Accessed February 16, 2019.
http://pqdtopen.proquest.com/#viewpdf?dispub=3300577.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Xiong, Hui. “Development and applications of nanofabricated electrodes for scanning electrochemical microscopy.” 2008. Web. 16 Feb 2019.
Vancouver:
Xiong H. Development and applications of nanofabricated electrodes for scanning electrochemical microscopy. [Internet] [Thesis]. University of Pittsburgh; 2008. [cited 2019 Feb 16].
Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3300577.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Xiong H. Development and applications of nanofabricated electrodes for scanning electrochemical microscopy. [Thesis]. University of Pittsburgh; 2008. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3300577
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Purdue University
20.
Bowers, Jeremiah J.
Gas phase analysis of biomolecular ions in a quadrupole ion trap| Protein analysis and instrumentation.
Degree: 2010, Purdue University
URL: http://pqdtopen.proquest.com/#viewpdf?dispub=3413831
► Ion/ion reactions were performed in a quadrupole mass spectrometer in order to facilitate top down protein identification, charge reduction, and charge inversion. The first…
(more)
▼ Ion/ion reactions were performed in a quadrupole mass spectrometer in order to facilitate top down protein identification, charge reduction, and charge inversion. The first portion of the dissertation is focused on original instrumental development of an electrospray interface optimized to efficiently transmit ions generated from electrospray through the ring electrode. Additionally, emitter voltage manipulation schemes to enable more robust ion/ion reaction capacity from duel emitters on a single source were also developed. The second portion of the dissertation focuses on a series of proton transfer ion/ion reactions that are used for distinctly different applications. A top-down protein identification strategy for complex protein mixtures was derived from post ion/ion mass spectra formed from a series of ion-parking, collision-induced dissociation, and proton transfer reaction steps. Simple proton hydrate clusters were utilized to facilitate proton transfer reactions that successfully charge reduced intact Arixtra molecules by partitioning some portion of the ion/ion reaction exothermicity into the desolvation of the proton hydrates. Finally, a sequential ion/ion reaction scheme was used to generate radical [M-2H]-. protein anions that were charge inverted [M]+. cations via a proton transfer reaction.
Subjects/Keywords: Chemistry; Analytical
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Bowers, J. J. (2010). Gas phase analysis of biomolecular ions in a quadrupole ion trap| Protein analysis and instrumentation. (Thesis). Purdue University. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=3413831
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Bowers, Jeremiah J. “Gas phase analysis of biomolecular ions in a quadrupole ion trap| Protein analysis and instrumentation.” 2010. Thesis, Purdue University. Accessed February 16, 2019.
http://pqdtopen.proquest.com/#viewpdf?dispub=3413831.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Bowers, Jeremiah J. “Gas phase analysis of biomolecular ions in a quadrupole ion trap| Protein analysis and instrumentation.” 2010. Web. 16 Feb 2019.
Vancouver:
Bowers JJ. Gas phase analysis of biomolecular ions in a quadrupole ion trap| Protein analysis and instrumentation. [Internet] [Thesis]. Purdue University; 2010. [cited 2019 Feb 16].
Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3413831.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Bowers JJ. Gas phase analysis of biomolecular ions in a quadrupole ion trap| Protein analysis and instrumentation. [Thesis]. Purdue University; 2010. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3413831
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Temple University
21.
Ganti, Satyakala.
Development of HPLC methods for pharmaceutically relevant molecules; method transfer to UPLC| Comparing methods statistically for equivalence.
Degree: 2011, Temple University
URL: http://pqdtopen.proquest.com/#viewpdf?dispub=3440078
► High Pressure Liquid Chromatography (HPLC) is a well-known and widely used analytical technique which is prevalent throughout the pharmaceutical industry as a research tool.…
(more)
▼ High Pressure Liquid Chromatography (HPLC) is a well-known and widely used analytical technique which is prevalent throughout the pharmaceutical industry as a research tool. Despite its prominence HPLC possesses some disadvantages, most notably slow analysis time and large consumption of organic solvents. Ultra Pressure Liquid Chromatography (UPLC) is a relatively new technique which offers the same separation capabilities of HPLC with the added benefits of reduced run time and lower solvent consumption. One of the key developments which facilitate the new UPLC technology is sub 2-μm particles used as column packing material. These particles allow for higher operating pressures and increased flow rates while still providing strong separation. Although UPLC technology has been available since early 2000, few laboratories have embraced the new technology as an alternative to HPLC. Besides the resistance to investing in new capital, another major roadblock is converting existing HPLC methodology to UPLC without disruption. This research provides a framework for converting existing HPLC methods to UPLC. An existing HPLC method for analysis of Galantamine hydrobromide was converted to UPLC and validated according to ICH guidelines. A series of statistical evaluations on the validation data were performed to prove the equivalency between the original HPLC and the new UPLC method. This research presents this novel statistical strategy which can be applied to any two methodologies to determine parity.
Subjects/Keywords: Chemistry; Analytical
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Ganti, S. (2011). Development of HPLC methods for pharmaceutically relevant molecules; method transfer to UPLC| Comparing methods statistically for equivalence. (Thesis). Temple University. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=3440078
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ganti, Satyakala. “Development of HPLC methods for pharmaceutically relevant molecules; method transfer to UPLC| Comparing methods statistically for equivalence.” 2011. Thesis, Temple University. Accessed February 16, 2019.
http://pqdtopen.proquest.com/#viewpdf?dispub=3440078.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ganti, Satyakala. “Development of HPLC methods for pharmaceutically relevant molecules; method transfer to UPLC| Comparing methods statistically for equivalence.” 2011. Web. 16 Feb 2019.
Vancouver:
Ganti S. Development of HPLC methods for pharmaceutically relevant molecules; method transfer to UPLC| Comparing methods statistically for equivalence. [Internet] [Thesis]. Temple University; 2011. [cited 2019 Feb 16].
Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3440078.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ganti S. Development of HPLC methods for pharmaceutically relevant molecules; method transfer to UPLC| Comparing methods statistically for equivalence. [Thesis]. Temple University; 2011. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3440078
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Delaware
22.
Roach, Carol A.
Multivariate analysis of time resolved emission spectroscopies for polymer and protein studies.
Degree: 2011, University of Delaware
URL: http://pqdtopen.proquest.com/#viewpdf?dispub=3443240
► Time resolved emission spectroscopy experiments have numerous applications within the physical sciences. For example, intrinsic and extrinsic fluorescent molecules are used in chemical and…
(more)
▼ Time resolved emission spectroscopy experiments have numerous applications within the physical sciences. For example, intrinsic and extrinsic fluorescent molecules are used in chemical and biological assays, monitoring of reactions, and imaging of structures. The need to study chemical and biological systems of greater complexity with fluorescent molecules has required the development of more sophisticated time resolved emission spectroscopic instruments, experimental techniques, and data analysis methods. Current research continues to provide new information on fundamental principles and properties of fluorescent molecules, information that is critical to proper interpretation of data generated by these more complex experiments. Chapter 4 of this dissertation discusses the development of a new instrument capable of performing full-spectrum fluorescence correlation spectroscopy. In Chapter 1 the advantages of full-spectrum fluorescence correlation spectroscopy, as well as proper interpretation of tryptophan fluorescence performed in Chapter 5, are discussed with conclusions, limitations, and concepts for future experiments presented in Chapter 6. Chapters 2 and 3 provide a background on fluorescence principles and multivariate analysis techniques as they apply to full-spectrum fluorescence correlation spectroscopy, and experiments involving tryptophan fluorescence interpretation that are presented in Chapter 5. For the first time, full spectra are collected during the course of fluorescence correlation spectroscopy measurements, allowing multivariate analysis to be applied to the data. Measurement of instrumental parameters for full-spectrum fluorescence correlation spectroscopy such as observation volume size, two-photon excitation capabilities, concentration limitations, and the range of observable diffusion coefficients are performed and compared to traditional fluorescence correlation spectroscopy instruments. An experimental application of the instrument to fluorescently labeled polystyrene particle diffusion in a polymer solution with a second fluorescent dye is shown. Separation of the two overlapped fluorescence spectra in this mixture by multivariate analysis is performed and diffusion coefficients are calculated. The results show that spectral resolution obtained by multivariate analysis of data collected with this novel instrument provides a method to reduce cross-talk in multicolor fluorescence correlation spectroscopy experiments. Experiments involving photokinetic matrix decomposition of time resolved emission decay matrices collected for the amino acid tryptophan and peptide mastoparan-X are performed. Tryptophan is a microenvironment sensitive fluorescent molecule that may naturally occur in proteins. The microenvironment sensitivity of tryptophan has been exploited to imply structural changes of proteins in time resolved fluorescence experiments, however tryptophan fluorescence photokinetics are complex. A controversy exists on the proper interpretation and use of…
Subjects/Keywords: Chemistry; Analytical
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Roach, C. A. (2011). Multivariate analysis of time resolved emission spectroscopies for polymer and protein studies. (Thesis). University of Delaware. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=3443240
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Roach, Carol A. “Multivariate analysis of time resolved emission spectroscopies for polymer and protein studies.” 2011. Thesis, University of Delaware. Accessed February 16, 2019.
http://pqdtopen.proquest.com/#viewpdf?dispub=3443240.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Roach, Carol A. “Multivariate analysis of time resolved emission spectroscopies for polymer and protein studies.” 2011. Web. 16 Feb 2019.
Vancouver:
Roach CA. Multivariate analysis of time resolved emission spectroscopies for polymer and protein studies. [Internet] [Thesis]. University of Delaware; 2011. [cited 2019 Feb 16].
Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3443240.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Roach CA. Multivariate analysis of time resolved emission spectroscopies for polymer and protein studies. [Thesis]. University of Delaware; 2011. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3443240
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
23.
Cherok, Courtney Alexandra.
Nebulizer comparison studies and their effects on plasma chemistry in inductively coupled plasma elemental atom and ion emission profiles utilizing acousto-optic tunable filter-hyperspectral imaging.
Degree: 2009, Northern Illinois University
URL: http://pqdtopen.proquest.com/#viewpdf?dispub=3369796
► An inductively coupled plasma acousto-optic tunable filter hyperspectral imaging (ICP-AOTF-HSI) system was used to examine multiple nebulizers with respect to strontium ionization and atomization…
(more)
▼ An inductively coupled plasma acousto-optic tunable filter hyperspectral imaging (ICP-AOTF-HSI) system was used to examine multiple nebulizers with respect to strontium ionization and atomization chemistry in an ICP. A charge-coupled-device (CCD) camera was used to take plasma analytical line and background images of strontium emissions, in order to obtain spatially resolved elemental emission images. This information allowed for data manipulation to elucidate plasma chemistry processes. The resulting analyte emissions were monitored while multiple parameters were altered such as ICP power, AOTF power, liquid analyte uptake flow rate (Q1), nebulizer gas flow rate (Qg), nebulization system, and the addition of easily ionizable elements. The resulting data were used to compare the behavior and efficiency of these nebulizers. The nebulizers used for these experiments were a Cetac U5000 AT+ ultrasonic nebulizer, Meinhard HEN 90A and HEN 170A high efficiency nebulizers with water-cooled Scott dual pass spray chambers, a Meinhard TR30-C1 pneumatic nebulizer with a water-cooled Scott dual pass spray chamber, and a Burgener Mira Mist enhanced parallel path nebulizer with a water-cooled Scott dual pass spray chamber. Additional experiments were done to determine the ability of the ICP-AOTF-HSI system to be used for elemental speciation. Y(I), Y(II), and YO emissions were found and monitored at 643.500, 437.494, and 597.2 nm using both the ICP-AOTF-HSI system and an ICP-monochromator system while altering plasma parameters. The plasma parameters altered were nebulizer gas composition and ICP power. The yttrium emission from both systems were compared and contrasted. These data determined that the AOTF-HSI system could be used for elemental speciation.
Subjects/Keywords: Chemistry; Analytical
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APA ·
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Export
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APA (6th Edition):
Cherok, C. A. (2009). Nebulizer comparison studies and their effects on plasma chemistry in inductively coupled plasma elemental atom and ion emission profiles utilizing acousto-optic tunable filter-hyperspectral imaging. (Thesis). Northern Illinois University. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=3369796
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Cherok, Courtney Alexandra. “Nebulizer comparison studies and their effects on plasma chemistry in inductively coupled plasma elemental atom and ion emission profiles utilizing acousto-optic tunable filter-hyperspectral imaging.” 2009. Thesis, Northern Illinois University. Accessed February 16, 2019.
http://pqdtopen.proquest.com/#viewpdf?dispub=3369796.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Cherok, Courtney Alexandra. “Nebulizer comparison studies and their effects on plasma chemistry in inductively coupled plasma elemental atom and ion emission profiles utilizing acousto-optic tunable filter-hyperspectral imaging.” 2009. Web. 16 Feb 2019.
Vancouver:
Cherok CA. Nebulizer comparison studies and their effects on plasma chemistry in inductively coupled plasma elemental atom and ion emission profiles utilizing acousto-optic tunable filter-hyperspectral imaging. [Internet] [Thesis]. Northern Illinois University; 2009. [cited 2019 Feb 16].
Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3369796.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Cherok CA. Nebulizer comparison studies and their effects on plasma chemistry in inductively coupled plasma elemental atom and ion emission profiles utilizing acousto-optic tunable filter-hyperspectral imaging. [Thesis]. Northern Illinois University; 2009. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3369796
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
24.
Kirmiz, Crystal.
Methods for profiling glycans for disease marker discovery.
Degree: 2009, University of California, Davis
URL: http://pqdtopen.proquest.com/#viewpdf?dispub=3369931
► It was once believed that glycans were an unnecessary part of the cell, providing only fuel. Since then much research has shown that glycans…
(more)
▼ It was once believed that glycans were an unnecessary part of the cell, providing only fuel. Since then much research has shown that glycans are extremely important in biological processes. Glycans are carbohydrates that are bonded to proteins in the cell, are commonly on the outside surface of a cell, can be used for cell-cell communication, such as fertilization. The analysis of glycans is particularly difficult due to the complexity of possible structures. Unlike proteins which have a linear structure of amino acids, monosaccharides have many bonding positions and therefore can be branched leading to many positional and conformational isomers. To understand the function of glycans it is first critical to be able to identify the structures present. Many different technologies exist to assist in the identification of carbohydrates including high performance liquid chromatography and mass spectrometry. Breast cancer is the second most common cause of cancer mortality among women. Since the early symptoms of breast cancer are minimal, early detection is virtually impossible. By the time the breast cancer is diagnosed, it can be so advanced that it becomes untreatable. If a biomarker existed that could indicate the presence of cancer in the early stages it could be a significant breakthrough for diagnosticians. The work here utilizes glycans as a possible biomarker for breast cancer. Prostate cancer is the most common type of cancer found in American men. Either surgery or radiation therapy can be used to successfully treat prostate cancer in the early stages. However, once prostate cancer becomes metastatic, there is no current method known to cure it. In this work a comparison is made between glycans extracted from cells of prostate cancer cell lines to determine if variations are observed, in the hopes of discovering potential prostate cancer biomarkers. The study of serum glycans, in particular <i>N</i>-linked, is advantageous for disease marker discovery. Many methods are available for the study of <i>N</i>-linked glycans. Several of these methods are not viable for biological levels of glycans. A nano-LC/MS method is presented here to analyze underivatized biological levels of <i>N</i>-linked glycans for online nano-LC/MS analysis.
Subjects/Keywords: Chemistry; Analytical
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APA (6th Edition):
Kirmiz, C. (2009). Methods for profiling glycans for disease marker discovery. (Thesis). University of California, Davis. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=3369931
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Kirmiz, Crystal. “Methods for profiling glycans for disease marker discovery.” 2009. Thesis, University of California, Davis. Accessed February 16, 2019.
http://pqdtopen.proquest.com/#viewpdf?dispub=3369931.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Kirmiz, Crystal. “Methods for profiling glycans for disease marker discovery.” 2009. Web. 16 Feb 2019.
Vancouver:
Kirmiz C. Methods for profiling glycans for disease marker discovery. [Internet] [Thesis]. University of California, Davis; 2009. [cited 2019 Feb 16].
Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3369931.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Kirmiz C. Methods for profiling glycans for disease marker discovery. [Thesis]. University of California, Davis; 2009. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3369931
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
25.
Wooten, Marilyn Dehasse.
Electrochemical sensors based on enzymes, biopolymers and nanoparticles.
Degree: 2010, The University of Texas at San Antonio
URL: http://pqdtopen.proquest.com/#viewpdf?dispub=3403012
► The work described in this Dissertation includes three research projects, designed to make a contribution to the field of electrochemical sensors and biosensors. The…
(more)
▼ The work described in this Dissertation includes three research projects, designed to make a contribution to the field of electrochemical sensors and biosensors. The electrochemical, spectroscopic, and microscopic analyses were used to explain encountered phenomena. <b>The first project</b> (Chapter 3) describes a unique case of minimally destructive activation of carbon nanotubes (CNT) toward the electro-oxidation of dihydronicotinamide adenine dinucleotide (NADH). NADH is an important biomolecule because it acts as a co-factor of hundreds of dehydrogenase enzymes and a biomarker of metabolic disorders. The proof-of-concept experiments are also presented for the development of new reagentless biosensors based on the activated CNT and NAD+-dependent dehydrogenases. <b>The second project</b> (Chapter 4) is focused on the non-enzymatic direct determination of glucose at gold electrodes in physiologically relevant neutral buffer solutions. Electrochemical determination of glucose at the conventional gold disc electrodes is compared with that at electrodes based on gold nanoparticles and gold nanostructured films. In addition, the effects of the ionic composition of a solution on the electrochemical determination of glucose at gold surfaces are also documented. <b>The third project</b> (Chapter 5) probes the role of the direct electron transfer (DET) between the enzyme glucose oxidase and CNT in electrochemical biosensing of glucose. It shows that the proper understanding of the mechanistic aspects of biosensing is critically important to the progress in development of a new generation of electrochemical devices including the mediatorless electrochemical biosensors and DET-based biological fuel cells.
Subjects/Keywords: Chemistry; Analytical
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APA (6th Edition):
Wooten, M. D. (2010). Electrochemical sensors based on enzymes, biopolymers and nanoparticles. (Thesis). The University of Texas at San Antonio. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=3403012
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Wooten, Marilyn Dehasse. “Electrochemical sensors based on enzymes, biopolymers and nanoparticles.” 2010. Thesis, The University of Texas at San Antonio. Accessed February 16, 2019.
http://pqdtopen.proquest.com/#viewpdf?dispub=3403012.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Wooten, Marilyn Dehasse. “Electrochemical sensors based on enzymes, biopolymers and nanoparticles.” 2010. Web. 16 Feb 2019.
Vancouver:
Wooten MD. Electrochemical sensors based on enzymes, biopolymers and nanoparticles. [Internet] [Thesis]. The University of Texas at San Antonio; 2010. [cited 2019 Feb 16].
Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3403012.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Wooten MD. Electrochemical sensors based on enzymes, biopolymers and nanoparticles. [Thesis]. The University of Texas at San Antonio; 2010. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3403012
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
26.
Schmidt, Morgan Steele.
Plasma Spectroscopic Techniques Applied to Biological and Environmental Matrices.
Degree: PhD, Chemistry and Biochemistry, 2012, U of Denver
URL: https://digitalcommons.du.edu/etd/921
► The purpose of this research was to apply the use of direct ablation plasma spectroscopic techniques, including spark-induced breakdown spectroscopy (SIBS) and laser-induced breakdown…
(more)
▼ The purpose of this research was to apply the use of direct ablation plasma spectroscopic techniques, including spark-induced breakdown spectroscopy (SIBS) and laser-induced breakdown spectroscopy (LIBS), to a variety of environmental matrices. These were applied to two different
analytical problems. SIBS instrumentation was adapted in order to develop a fieldable monitor for the measurement of carbon in soil. SIBS spectra in the 200 nm to 400 nm region of several soils were collected, and the neutral carbon line (247.85 nm) was compared to total carbon concentration determined by standard dry combustion analysis. Additionally, Fe and Si were evaluated in a multivariate model in order to determine their impacts on the model's predictive power for total carbon concentrations. The results indicate that SIBS is a viable method to quantify total carbon levels in soils; obtaining a good correlation between measured and predicated carbon in soils. These results indicate that multivariate analysis can be used to construct a calibration model for SIBS soil spectra, and SIBS is a promising method for the determination of total soil carbon.
SIBS was also applied to the study of biological warfare agent simulants. Elemental compositions (determined independently) of bioaerosol samples were compared to the SIBS atomic (Ca, Al, Fe and Si) and molecular (CN, N
2 and OH) emission signals. Results indicate a linear relationship between the temporally integrated emission strength and the concentration of the associated element.
Finally, LIBS signals of hematite were analyzed under low pressures of pure CO
2 and compared with signals acquired with a mixture of CO
2, N
2 and Ar, which is representative of the Martian atmosphere. This research was in response to the potential use of LIBS instrumentation on the Martian surface and to the challenges associated with these measurements. Changes in Ca, Fe and Al lineshapes observed in the LIBS spectra at different gas compositions and pressures were studied. It was observed that the size of the plasma formed on the hematite changed in a non-linear way as a function of decreasing pressure in a CO
2 atmosphere and a simulated Martian atmosphere.
Advisors/Committee Members: Keith E. Miller, Ph.D..
Subjects/Keywords: Analytical Chemistry
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APA (6th Edition):
Schmidt, M. S. (2012). Plasma Spectroscopic Techniques Applied to Biological and Environmental Matrices. (Doctoral Dissertation). U of Denver. Retrieved from https://digitalcommons.du.edu/etd/921
Chicago Manual of Style (16th Edition):
Schmidt, Morgan Steele. “Plasma Spectroscopic Techniques Applied to Biological and Environmental Matrices.” 2012. Doctoral Dissertation, U of Denver. Accessed February 16, 2019.
https://digitalcommons.du.edu/etd/921.
MLA Handbook (7th Edition):
Schmidt, Morgan Steele. “Plasma Spectroscopic Techniques Applied to Biological and Environmental Matrices.” 2012. Web. 16 Feb 2019.
Vancouver:
Schmidt MS. Plasma Spectroscopic Techniques Applied to Biological and Environmental Matrices. [Internet] [Doctoral dissertation]. U of Denver; 2012. [cited 2019 Feb 16].
Available from: https://digitalcommons.du.edu/etd/921.
Council of Science Editors:
Schmidt MS. Plasma Spectroscopic Techniques Applied to Biological and Environmental Matrices. [Doctoral Dissertation]. U of Denver; 2012. Available from: https://digitalcommons.du.edu/etd/921
Temple University
27.
Varma, Deepti.
EVALUATION OF THE OXIDATIVE METABOLITES OF ARACHIDONIC ACID AND THEIR IMPLICATION IN THE PROGRESSION OF INFLAMMATORY DISEASES: HYPERTENSION AND RHEUMATOID ARTHRITIS.
Degree: PhD, 2012, Temple University
URL: http://digital.library.temple.edu/u?/p245801coll10,282122
► Chemistry
Inflammation is implicated in diseases such as hypertension and rheumatoid arthritis (RA). A mechanistic understanding of inflammatory processes as it relates to the disease…
(more)
▼ Chemistry
Inflammation is implicated in diseases such as hypertension and rheumatoid arthritis (RA). A mechanistic understanding of inflammatory processes as it relates to the disease state and injury needs to be developed. Specifically, the role and modulation of inflammation needs to be assessed, as well as the mechanism that produces arachidonic acid (AA) metabolites (eicosanoids). Eicosanoids are specific biomarkers of inflammation. Their biosynthesis from arachidonic acid can be catalyzed by either free radicals or enzymes such as lipoxygenases (LOX), cyclooxygenase-2 (COX-2) and cytochrome P450. Depending on the pathway or parent molecule, different distributions of eicosanoids are found. The oxidation of AA gives hydroxyeicosatetraenoic acids (HETEs), dihydroxyeicosatetraenoic acids (DHETEs), epoxyeicosatetraenoic acids (EETs), prostaglandins (PGs), isoprostanes (Isops) and thromboxanes (TXs). It is our hypothesis that AA metabolites will help in understanding the progression of inflammatory diseases. To confirm this hypothesis, analytical methods including HPLC-UV and LC-MS were developed. The developed and validated HPLC method was applied to study the effect of acute exercise on prostanoids in hypertensive African American subjects. It was our theory that urinary 6-keto PGF1&alpha and 11-dehydro TXB2 can be used to assess the role of exercise in hypertension. Moreover, we assume that 8-iso PGF2 levels can be used as an indicator to determine the relationship of oxidative stress and endothelial dysfunction in hypertension. The HPLC method involved separating urinary 8-iso PGF2, PGE2, PGD2, PGF2, 6-keto PGF1&alpha and 11-dehydro TXB2 on a SymmetryShield Rp18 column (250mm ¡Á 4.6mm) by an isocratic elution of 17 mM phosphoric acid and acetonitrile in the ratio of 65:35 and at a flow rate of 1.3 ml/min. The wavelength used for detection was 196 nm. Specificity was confirmed by LC-MS. The method was fully validated and was found to be having sufficient sensitivity (limit of quantification - 7.5 ng - 30 ng) for many biological matrices and applications. The accuracy and precision were within bioanalytical method validation limits (90.3 to 112.8 % and RSD < 10%, respectively) and the method was linear over three orders of magnitude. In addition, a HPLC-UV method for the simultaneous determination of urinary creatinine and prostanoids was also developed and validated as it is necessary to monitor creatinine levels in addition to biomarkers when the measurement is done in urine. The method was found to be linear over three orders of magnitude and is sensitive enough for the analysis of creatinine and prostanoids in urine. The advantage of this method was that one can determine the levels of these prostanoids normalized by urinary creatinine in a single analysis and in less than 17 min. The LC- ESI (electrospray ionization) MS method, on the other hand was used to determine the role of HETEs in the initiation, progression and resolution phases of inflammation in RA. It is our assumption that 12/15 HETE can be used…
Advisors/Committee Members: Varnum, Susan, Stanley, Robert J., Zdilla, Michael J., Brown, Michael D..
Subjects/Keywords: Analytical chemistry
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APA ·
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Export
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APA (6th Edition):
Varma, D. (2012). EVALUATION OF THE OXIDATIVE METABOLITES OF ARACHIDONIC ACID AND THEIR IMPLICATION IN THE PROGRESSION OF INFLAMMATORY DISEASES: HYPERTENSION AND RHEUMATOID ARTHRITIS. (Doctoral Dissertation). Temple University. Retrieved from http://digital.library.temple.edu/u?/p245801coll10,282122
Chicago Manual of Style (16th Edition):
Varma, Deepti. “EVALUATION OF THE OXIDATIVE METABOLITES OF ARACHIDONIC ACID AND THEIR IMPLICATION IN THE PROGRESSION OF INFLAMMATORY DISEASES: HYPERTENSION AND RHEUMATOID ARTHRITIS.” 2012. Doctoral Dissertation, Temple University. Accessed February 16, 2019.
http://digital.library.temple.edu/u?/p245801coll10,282122.
MLA Handbook (7th Edition):
Varma, Deepti. “EVALUATION OF THE OXIDATIVE METABOLITES OF ARACHIDONIC ACID AND THEIR IMPLICATION IN THE PROGRESSION OF INFLAMMATORY DISEASES: HYPERTENSION AND RHEUMATOID ARTHRITIS.” 2012. Web. 16 Feb 2019.
Vancouver:
Varma D. EVALUATION OF THE OXIDATIVE METABOLITES OF ARACHIDONIC ACID AND THEIR IMPLICATION IN THE PROGRESSION OF INFLAMMATORY DISEASES: HYPERTENSION AND RHEUMATOID ARTHRITIS. [Internet] [Doctoral dissertation]. Temple University; 2012. [cited 2019 Feb 16].
Available from: http://digital.library.temple.edu/u?/p245801coll10,282122.
Council of Science Editors:
Varma D. EVALUATION OF THE OXIDATIVE METABOLITES OF ARACHIDONIC ACID AND THEIR IMPLICATION IN THE PROGRESSION OF INFLAMMATORY DISEASES: HYPERTENSION AND RHEUMATOID ARTHRITIS. [Doctoral Dissertation]. Temple University; 2012. Available from: http://digital.library.temple.edu/u?/p245801coll10,282122
28.
Alluhayb, Abdullah H.
The Analysis of Flavor Compounds in Green Tea Using Ice Concentration Linked with Extractive Stirrer (Icecles).
Degree: MS, Chemistry and Biochemistry, 2017, South Dakota State University
URL: http://openprairie.sdstate.edu/etd/1161
► Sample preparation of target components from food samples is one of the most difficult steps in this type of analysis. Many extraction techniques have…
(more)
▼ Sample preparation of target components from food samples is one of the most difficult steps in this type of analysis. Many extraction techniques have been used for this purpose, such as liquid-liquid extraction (LLE), accelerated solvent extraction (ASE), microwave-assisted extraction (MAE), solid-phase extraction (SPE), solid-phase microextraction (SPME), and stir bar sorptive extraction (SBSE). Although each of these techniques works well, they each have a number of disadvantages, including selectivity, relatively high cost, long preparation time, and matrix effects. Ice concentration linked with extractive stirrer (ICECLES) is a promising new sample preparation technique, especially for the extraction of relatively polar compounds, which may prove to have widespread applicability for
analytical sample preparation. ICECLES was used to prepare green tea for flavor analysis by gas chromatography-mass spectrometry (GC-MS). ICECLES produce 301 constituents, the vast majority with stronger signal to noise ratios than the 245 components found using SBSE. Therefore, 56 extra constituents were detectable via ICECLES alone, including some very important flavor compounds such as furfural, eugenol, 2-methylpyrazine, phenethyl alcohol, α-terpineol, and 2,6-dimethoxyphenol. Overall, ICECLES sample preparation followed by GC-MS showed higher extraction efficiencies for the vast majority of green tea flavor components, including relatively polar compounds, as compared to SBSE.
Advisors/Committee Members: Brian A. Logue.
Subjects/Keywords: Analytical Chemistry
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APA (6th Edition):
Alluhayb, A. H. (2017). The Analysis of Flavor Compounds in Green Tea Using Ice Concentration Linked with Extractive Stirrer (Icecles). (Masters Thesis). South Dakota State University. Retrieved from http://openprairie.sdstate.edu/etd/1161
Chicago Manual of Style (16th Edition):
Alluhayb, Abdullah H. “The Analysis of Flavor Compounds in Green Tea Using Ice Concentration Linked with Extractive Stirrer (Icecles).” 2017. Masters Thesis, South Dakota State University. Accessed February 16, 2019.
http://openprairie.sdstate.edu/etd/1161.
MLA Handbook (7th Edition):
Alluhayb, Abdullah H. “The Analysis of Flavor Compounds in Green Tea Using Ice Concentration Linked with Extractive Stirrer (Icecles).” 2017. Web. 16 Feb 2019.
Vancouver:
Alluhayb AH. The Analysis of Flavor Compounds in Green Tea Using Ice Concentration Linked with Extractive Stirrer (Icecles). [Internet] [Masters thesis]. South Dakota State University; 2017. [cited 2019 Feb 16].
Available from: http://openprairie.sdstate.edu/etd/1161.
Council of Science Editors:
Alluhayb AH. The Analysis of Flavor Compounds in Green Tea Using Ice Concentration Linked with Extractive Stirrer (Icecles). [Masters Thesis]. South Dakota State University; 2017. Available from: http://openprairie.sdstate.edu/etd/1161
29.
Bikki, Sree Divya.
Interaction Between Humic Acid and DNA.
Degree: MS, Analytical Chemistry, 2012, Governors State University
URL: http://opus.govst.edu/theses/1
► Humic acid (HA) are widely dispersed, naturally occurring biopolymers most commonly found in soil, drinking water, and plants. Humic acid is soluble in alkali…
(more)
▼ Humic acid (HA) are widely dispersed, naturally occurring biopolymers most commonly found in soil, drinking water, and plants. Humic acid is soluble in alkali and insoluble in an acidic medium or water. In the present study the interaction of Humic acid with DNA is determined by the calculation of the binding constant of humic acid with CT-DNA and the quenching phenomenon of humic acid in the presence of DNA is also studied using Stern Volmer Relationship.
Advisors/Committee Members: Patty Fu-Giles, Ph.D., Walter Henne, Jr., Ph.D., Karen D'Arcy, Ph.D..
Subjects/Keywords: Analytical Chemistry
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APA (6th Edition):
Bikki, S. D. (2012). Interaction Between Humic Acid and DNA. (Thesis). Governors State University. Retrieved from http://opus.govst.edu/theses/1
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Bikki, Sree Divya. “Interaction Between Humic Acid and DNA.” 2012. Thesis, Governors State University. Accessed February 16, 2019.
http://opus.govst.edu/theses/1.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Bikki, Sree Divya. “Interaction Between Humic Acid and DNA.” 2012. Web. 16 Feb 2019.
Vancouver:
Bikki SD. Interaction Between Humic Acid and DNA. [Internet] [Thesis]. Governors State University; 2012. [cited 2019 Feb 16].
Available from: http://opus.govst.edu/theses/1.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Bikki SD. Interaction Between Humic Acid and DNA. [Thesis]. Governors State University; 2012. Available from: http://opus.govst.edu/theses/1
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
30.
Kanduri, Gouthami.
Quantitative Determination of Acetylsalicyclic Acid and Acetaminophen by Q-NMR (Quantitative Nuclear Magnetic Resonance) Technique.
Degree: MS, Analytical Chemistry, 2012, Governors State University
URL: http://opus.govst.edu/theses/10
► The pharmaceutical industry mainly uses chromatographic techniques such as High Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC), to determine the quantity of the…
(more)
▼ The pharmaceutical industry mainly uses chromatographic techniques such as High Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC), to determine the quantity of the active ingredient and other material in the drugs. A large effort goes into developing methods using chromatography techniques. The method development and running HPLC and GC are time consuming. Methods need to be updated as the chromatographic columns and the instruments wear out. On the other hand, Nuclear Magnetic Resonance (NMR) technique is mainly used for qualitative analysis to determine the identity of compounds. However, Proton-NMR technique does provide quantitative information of compounds but to develop a method an internal standard of known concentration is required. We are using q-NMR (quantitative NMR) to quantitatively analyze acetylsalicylic acid and acetaminophen by Proton-NMR using diethyl ether as an internal standard. The area of NMR signals shows a linear relationship with the concentration. Acetylsalicylic acid and acetaminophen were chosen as a representative drug. The method is useful for a wide variety of drugs. Our future studies include using a variety of appropriate internal standards to determine concentrations of several organic compounds including various pharmaceutical drugs and petroleum products.
Advisors/Committee Members: Shailendra Kumar, Ph.D., Walter Henne, Jr., Ph.D., Karen D'Arcy, Ph.D..
Subjects/Keywords: Analytical Chemistry
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APA ·
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APA (6th Edition):
Kanduri, G. (2012). Quantitative Determination of Acetylsalicyclic Acid and Acetaminophen by Q-NMR (Quantitative Nuclear Magnetic Resonance) Technique. (Thesis). Governors State University. Retrieved from http://opus.govst.edu/theses/10
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Kanduri, Gouthami. “Quantitative Determination of Acetylsalicyclic Acid and Acetaminophen by Q-NMR (Quantitative Nuclear Magnetic Resonance) Technique.” 2012. Thesis, Governors State University. Accessed February 16, 2019.
http://opus.govst.edu/theses/10.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Kanduri, Gouthami. “Quantitative Determination of Acetylsalicyclic Acid and Acetaminophen by Q-NMR (Quantitative Nuclear Magnetic Resonance) Technique.” 2012. Web. 16 Feb 2019.
Vancouver:
Kanduri G. Quantitative Determination of Acetylsalicyclic Acid and Acetaminophen by Q-NMR (Quantitative Nuclear Magnetic Resonance) Technique. [Internet] [Thesis]. Governors State University; 2012. [cited 2019 Feb 16].
Available from: http://opus.govst.edu/theses/10.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Kanduri G. Quantitative Determination of Acetylsalicyclic Acid and Acetaminophen by Q-NMR (Quantitative Nuclear Magnetic Resonance) Technique. [Thesis]. Governors State University; 2012. Available from: http://opus.govst.edu/theses/10
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
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Open Access Theses and Dissertations
