You searched for subject:(Alginate).
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Victoria University of Wellington
1.
Abbott, Lynaire Jane.
Polysaccharide Content and Growth Rate of Lessonia Variegata J. Agardh: Investigating its Potential as a Commercial Species.
Degree: 2011, Victoria University of Wellington
URL: http://hdl.handle.net/10063/4527 
► The endemic brown alga Lessonia variegata has recently been shown to be four separate lineages. To determine differences between the four morphologically similar lineages, the…
(more)
▼ The endemic brown alga Lessonia variegata has recently been shown to be four separate lineages. To determine differences between the four morphologically similar lineages, the economically valuable polysaccharides
alginate and fucoidan were extracted and yields from each of the lineages were compared. In order to determine seasonal patterns in the yield of
alginate and fucoidan, and the growth rate within L.variegata, polysaccharides were extracted and the growth rate measured on a monthly basis from March 2010 until February 2011 on plants from the Wellington lineage. The
alginate and fucoidan yields were obtained via stepwise extraction with dilute acid and sodium carbonate as per the previously published methods of Usov et al. (1985). The growth rate of L. variegata from the Wellington lineage was assayed using the hole punch technique first described by Parke (1948). The yield of
alginate within the Wellington lineage of L. variegata fluctuated seasonally with the highest percent occurring in spring and summer 2010. The yield of fucoidan in the Wellington lineage was at its highest in mid-autumn and late spring 2010. Two different growth rates were detected for the Wellington lineage of L. variegata. There was a period of significantly high growth from late winter 2010 until late summer 2011.The Wellington lineage had the lowest yield of
alginate and the highest yield of fucoidan compared to the Northern lineage, the Kaikoura lineage and the Southern lineage. Based on the findings of this study, an appropriate harvest period for the Wellington lineage of L. variegata would be in early to mid-summer when polysaccharide yields and growth rates are high and the alga is vegetative.
Advisors/Committee Members: Zuccarello, Joe.
Subjects/Keywords: Algae; Fucoidan; Alginate
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APA (6th Edition):
Abbott, L. J. (2011). Polysaccharide Content and Growth Rate of Lessonia Variegata J. Agardh: Investigating its Potential as a Commercial Species. (Masters Thesis). Victoria University of Wellington. Retrieved from http://hdl.handle.net/10063/4527
Chicago Manual of Style (16th Edition):
Abbott, Lynaire Jane. “Polysaccharide Content and Growth Rate of Lessonia Variegata J. Agardh: Investigating its Potential as a Commercial Species.” 2011. Masters Thesis, Victoria University of Wellington. Accessed January 27, 2021.
http://hdl.handle.net/10063/4527.
MLA Handbook (7th Edition):
Abbott, Lynaire Jane. “Polysaccharide Content and Growth Rate of Lessonia Variegata J. Agardh: Investigating its Potential as a Commercial Species.” 2011. Web. 27 Jan 2021.
Vancouver:
Abbott LJ. Polysaccharide Content and Growth Rate of Lessonia Variegata J. Agardh: Investigating its Potential as a Commercial Species. [Internet] [Masters thesis]. Victoria University of Wellington; 2011. [cited 2021 Jan 27].
Available from: http://hdl.handle.net/10063/4527.
Council of Science Editors:
Abbott LJ. Polysaccharide Content and Growth Rate of Lessonia Variegata J. Agardh: Investigating its Potential as a Commercial Species. [Masters Thesis]. Victoria University of Wellington; 2011. Available from: http://hdl.handle.net/10063/4527
University of Otago
2.
Germoni, Leonardo Alexandro Piero.
The effect of alginate lyase on the antibiotic sensitivity of mucoid Pseudomonas aeruginosa biofilms
.
Degree: 2014, University of Otago
URL: http://hdl.handle.net/10523/4784
► Bacteria are single celled organisms that can live together in communities called biofilms. These biofilms consist of a structural matrix consisting of polysaccharide gel secreted…
(more)
▼ Bacteria are single celled organisms that can live together in communities called biofilms. These biofilms consist of a structural matrix consisting of polysaccharide gel secreted by the bacteria, with the bacteria living within the confines of the gel. Biofilms can arise on all sorts of surfaces, including those of medical catheters and equipment as well as the tissues of the body itself. Biofilms act as reservoirs of infection allowing bacteria to set up chronic infections that resist both the host immune system and antibiotics. People with compromised defences, such as cystic fibrosis (CF) sufferers, are particularly vulnerable to infection, especially by biofilm forming bacteria. The lungs of CF patients have thickened, dehydrated lung mucus that prevents the lungs from clearing inhaled foreign particles, including bacteria.
P. aeruginosa is an opportunistic pathogen of humans and one of the most common organisms that infect the lungs of individuals with CF. During chronic infection P. aeruginosa can overproduce
alginate, a capsular, highly viscous polysaccharide. This polysaccharide forms a thick capsule around the bacteria which retains water and also prevents complement-mediated killing and phagocytosis by neutrophils.
Alginate has been linked to antibiotic resistance by some researchers while others have shown
alginate plays almost no role in antibiotic resistance.
Alginate can be broken down by the enzyme
alginate lyase which eliminates the glycosidic bond between two sugar residues in the polysaccharide chain.
The aim of this study was to compare and characterise the effects of two different
alginate lyase enzymes on the structure of P. aeruginosa biofilms and the sensitivity of biofilm bacteria to antibiotics.
The
alginate lyases used in this study were a commercially available lyase from Sphingobacterium multivorans (SBAL) and the ALYI-III
alginate lyase from Sphingomonas sp. A1 (ALY) produced via recombinant gene expression in E. coli. The recombinant protein included a 6x Histidine tag on the N-terminal sequence and was purified using immobilised metal affinity chromatography with nickel ion coated beads.
Both enzyme preparations were characterised to determine basic kinetic parameters, the ability to degrade cation-
alginate gels and tolerance to thermal degradation. Enzyme activity was measured using UV spectroscopy and rheometry based assays. Following enzyme characterisation biofilms were grown both in multi-well plates and on coupons, and were treated with two different
alginate lyases along with carbenicillin and gentamicin antibiotics. Biofilm imaging techniques using a LIVE/DEAD cell viability stain to determine if lyases affected biofilm cell viability were trialled unsuccessfully.
Alginate lyase, at the concentrations used in the study did not increase antibiotic sensitivity. Treatment with
alginate lyase either had no effect on biofilm populations or caused a small but significant increase in viable cell number and overall biomass. The results suggested that the biofilm populations…
Advisors/Committee Members: Lamont, Iain (advisor).
Subjects/Keywords: Alginate;
Lyase;
Biofilms;
Pseudomonas
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APA (6th Edition):
Germoni, L. A. P. (2014). The effect of alginate lyase on the antibiotic sensitivity of mucoid Pseudomonas aeruginosa biofilms
. (Doctoral Dissertation). University of Otago. Retrieved from http://hdl.handle.net/10523/4784
Chicago Manual of Style (16th Edition):
Germoni, Leonardo Alexandro Piero. “The effect of alginate lyase on the antibiotic sensitivity of mucoid Pseudomonas aeruginosa biofilms
.” 2014. Doctoral Dissertation, University of Otago. Accessed January 27, 2021.
http://hdl.handle.net/10523/4784.
MLA Handbook (7th Edition):
Germoni, Leonardo Alexandro Piero. “The effect of alginate lyase on the antibiotic sensitivity of mucoid Pseudomonas aeruginosa biofilms
.” 2014. Web. 27 Jan 2021.
Vancouver:
Germoni LAP. The effect of alginate lyase on the antibiotic sensitivity of mucoid Pseudomonas aeruginosa biofilms
. [Internet] [Doctoral dissertation]. University of Otago; 2014. [cited 2021 Jan 27].
Available from: http://hdl.handle.net/10523/4784.
Council of Science Editors:
Germoni LAP. The effect of alginate lyase on the antibiotic sensitivity of mucoid Pseudomonas aeruginosa biofilms
. [Doctoral Dissertation]. University of Otago; 2014. Available from: http://hdl.handle.net/10523/4784
University of Saskatchewan
3.
Yasmin, Farhana 1990-.
DEVELOPMENT OF ALGINATE NANOSPHERES AS A PROTEIN DELIVERY DEVICE FOR CARTILAGE TISSUE ENGINEERING.
Degree: 2018, University of Saskatchewan
URL: http://hdl.handle.net/10388/11546
► Delivery of bioactive proteins is a valuable strategy in cartilage tissue engineering (CTE) because of their ability to regulate the gene expression and extracellular matrix…
(more)
▼ Delivery of bioactive proteins is a valuable strategy in cartilage tissue engineering (CTE) because of their ability to regulate the gene expression and extracellular matrix (ECM) production of engineered cartilage. This, however, has been challenged by the nature of bioactive proteins including their instability, poor tissue penetration ability, short half-life and a relatively high price. Development of nanospheres as a protein delivery device should solve these issues by promoting the temporal and spatial presentation of such bioactive proteins in a defined target for the enhanced half-life time and effectiveness. Among various polymer-based micro/nanospheres,
alginate micro/nanospheres have been widely used as a protein delivery device because of their mild and easy protein encapsulation process, inert nature, non-toxicity and biocompatibility. However, one of the major limitations of using
alginate as a protein delivery device is its high initial burst release due to its high porosity and instability if exposed in a higher pH release media. To address these issues, this study aimed to develop the protein loaded
alginate nanospheres as a delivery device with a reduced initial burst release. The hypothesis was, “Increasing the
alginate concentration, cross-linking time or drying time reduces the initial burst release independently of associated changes to the size and number of nanospheres.” Bovine Serum Albumin (BSA) was used as a model protein in this study to evaluate the performance of
alginate nanospheres as a protein delivery device, while protein loaded
alginate nanospheres were prepared via a combination of water-in-oil emulsification and external gelation method. The process parameters tested to reduce the initial burst release include,
alginate concentration, cross-linking time and drying time. The effects of these process parameters on the nanosphere size and distribution pattern, relative number of microspheres, initial burst release, protein release kinetics and encapsulation efficiency (EE%) were investigated. Also, if the change in size and relative number of nanospheres by varying these process parameters affected the initial burst release was investigated. It has been illustrated that by properly increasing the
alginate concentration, cross-linking time and drying time it was possible to reduce initial burst release by 13%, and among various process parameters only the
alginate concentration showed a significant effect on the initial burst release, when considered alone. Also, it was confirmed that during determination of the effect of various process parameters the relative number of nanospheres significantly affected the initial burst release. Taken together, this study demonstrates that regulating various process parameters is a mean to reduce initial burst release of
alginate nanospheres, urging more studies on
alginate nanospheres for their potential application in CTE.
Advisors/Committee Members: Chen, Daniel, Eames, Brian, Zhu, Ning, Boughner, Julia, Wasan, Ellen.
Subjects/Keywords: alginate nanospheres; protein delivery
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APA (6th Edition):
Yasmin, F. 1. (2018). DEVELOPMENT OF ALGINATE NANOSPHERES AS A PROTEIN DELIVERY DEVICE FOR CARTILAGE TISSUE ENGINEERING. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/11546
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Yasmin, Farhana 1990-. “DEVELOPMENT OF ALGINATE NANOSPHERES AS A PROTEIN DELIVERY DEVICE FOR CARTILAGE TISSUE ENGINEERING.” 2018. Thesis, University of Saskatchewan. Accessed January 27, 2021.
http://hdl.handle.net/10388/11546.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Yasmin, Farhana 1990-. “DEVELOPMENT OF ALGINATE NANOSPHERES AS A PROTEIN DELIVERY DEVICE FOR CARTILAGE TISSUE ENGINEERING.” 2018. Web. 27 Jan 2021.
Vancouver:
Yasmin F1. DEVELOPMENT OF ALGINATE NANOSPHERES AS A PROTEIN DELIVERY DEVICE FOR CARTILAGE TISSUE ENGINEERING. [Internet] [Thesis]. University of Saskatchewan; 2018. [cited 2021 Jan 27].
Available from: http://hdl.handle.net/10388/11546.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Yasmin F1. DEVELOPMENT OF ALGINATE NANOSPHERES AS A PROTEIN DELIVERY DEVICE FOR CARTILAGE TISSUE ENGINEERING. [Thesis]. University of Saskatchewan; 2018. Available from: http://hdl.handle.net/10388/11546
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Penn State University
4.
Lalpuria, Minal Prakash.
Mechanisms and kinetics of nisin release from calcium alginate films.
Degree: 2013, Penn State University
URL: https://submit-etda.libraries.psu.edu/catalog/14660
► Several studies have shown that calcium alginate matrices containing nisin effectively inhibits microbial growth in various foods such as beef, poultry, milk, and mushrooms, but…
(more)
▼ Several studies have shown that calcium
alginate matrices containing nisin effectively inhibits microbial growth in various foods such as beef, poultry, milk, and mushrooms, but they only present qualitative information on overall effectiveness of these systems on microbial inhibition. In order to design an effective antimicrobial packaging system for commercial applications, it is important to study the impact of various factors that govern the structure and properties of calcium
alginate films and the diffusion of nisin from these films. It is known that nisin is a cationic polypeptide, whereas
alginate is an anionic polymer. Ionic interactions between nisin and
alginate might hamper the release of nisin from the
alginate matrix, leading to reduced antimicrobial activity. Hence, it is essential to characterize interactions between nisin and
alginate and to investigate the antimicrobial activity of nisin released from calcium
alginate films. The overall goal of this research was to study the mechanisms and kinetics of nisin release from calcium
alginate films. The objectives were to: (a) modify agar diffusion assay for better quantification of nisin; (b) determine factors affecting the release of nisin from calcium
alginate films; (c) investigate the interaction between nisin and
alginate; (d) determine the kinetics of release; (e) evaluate the physical, mechanical and optical properties of these films; and (f) investigate antimicrobial activity of the released nisin.
Agar diffusion bioassay, using Micrococcus luteus, is a commonly used method for quantification of nisin concentration. However, the correlation between inhibition zone and nisin concentration is not well established at higher nisin concentrations. The objective of this study was to investigate the effect of different well size (small and large) and pre-diffusion times (0, 24, 48 and 72 h) at 4°C on the sensitivity, precision and accuracy of nisin quantification. Regression analysis indicate that small wells were better than large wells because of smaller standard deviation, higher predictive accuracy, and better discrimination between mean inhibition zones at neighboring nisin concentrations. Statistical analysis by Tukey’s test showed that pre-diffusion resulted in significantly different inhibition zones at different nisin concentations. Pre-diffusion also improved sensitivity of the assay at different nisin concentrations. Different regression models were considered to explore the relationship between inhibition zone and nisin concentration for all pre-diffusion times. A spline model was the best fit model and 48 h was the best pre-diffusion time.
Factors affecting the release of nisin from calcium
alginate films were studied by the disc agar diffusion bioassay. Calcium
alginate films were formed by two methods – cross-linking before drying (CBD) and cross-linking after drying (CAD) – using various
alginate compositions, cross-linking times and Ca2+concentrations. The method of film formation, cross-linking time, and Ca2+concentration had a…
Advisors/Committee Members: Ramaswamy C Anantheswaran, Dissertation Advisor/Co-Advisor, Ramaswamy C Anantheswaran, Committee Chair/Co-Chair, John Coupland, Committee Member, Ali Demirci, Committee Member.
Subjects/Keywords: antimicrobial packaging; nisin; alginate; diffusion
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APA (6th Edition):
Lalpuria, M. P. (2013). Mechanisms and kinetics of nisin release from calcium alginate films. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/14660
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Lalpuria, Minal Prakash. “Mechanisms and kinetics of nisin release from calcium alginate films.” 2013. Thesis, Penn State University. Accessed January 27, 2021.
https://submit-etda.libraries.psu.edu/catalog/14660.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Lalpuria, Minal Prakash. “Mechanisms and kinetics of nisin release from calcium alginate films.” 2013. Web. 27 Jan 2021.
Vancouver:
Lalpuria MP. Mechanisms and kinetics of nisin release from calcium alginate films. [Internet] [Thesis]. Penn State University; 2013. [cited 2021 Jan 27].
Available from: https://submit-etda.libraries.psu.edu/catalog/14660.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Lalpuria MP. Mechanisms and kinetics of nisin release from calcium alginate films. [Thesis]. Penn State University; 2013. Available from: https://submit-etda.libraries.psu.edu/catalog/14660
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Connecticut
5.
Podany, Michael V, Mr.
Investigation of In-situ Immobilization of Contaminated Sediments using Alginate Gels for the Reduction of Ecological Risk.
Degree: MS, Environmental Engineering, 2012, University of Connecticut
URL: https://opencommons.uconn.edu/gs_theses/371
► Remediation methods for contaminated sediments include dredging, treatment and disposal and sediment isolation through capping. These methods are costly and adversely impact local ecosystems,…
(more)
▼ Remediation methods for contaminated sediments include dredging, treatment and disposal and sediment isolation through capping. These methods are costly and adversely impact local ecosystems, in some cases causing major disturbances to the sediment bed and water column. A new remediation technique is proposed for slowly biodegradable contaminants using
alginate hydrogels as a binding agent of sediment. The rational is that sediment particles bound in
alginate hydrogel are less likely to be resuspended and the treatment is less disruptive than capping. Here we show addition of
alginate increased the shear strength of sediment comprised of defined particle sizes. The increase in strength becomes more pronounced as the normal stress is increased.
Alginate's ability to trap contaminates in its structure was found to be pH-dependent, with more effective contaminant sequestration at lower pH. . Lab scale tests on contaminant tranpsort from
alginate bound sediment showed Naphthalene diffusion from sediment was reduced.
Advisors/Committee Members: Leslie Shor, Baikun Li, Jeffery McCutcheon.
Subjects/Keywords: alginate; sediment; remediation; in-situ
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APA (6th Edition):
Podany, Michael V, M. (2012). Investigation of In-situ Immobilization of Contaminated Sediments using Alginate Gels for the Reduction of Ecological Risk. (Masters Thesis). University of Connecticut. Retrieved from https://opencommons.uconn.edu/gs_theses/371
Chicago Manual of Style (16th Edition):
Podany, Michael V, Mr. “Investigation of In-situ Immobilization of Contaminated Sediments using Alginate Gels for the Reduction of Ecological Risk.” 2012. Masters Thesis, University of Connecticut. Accessed January 27, 2021.
https://opencommons.uconn.edu/gs_theses/371.
MLA Handbook (7th Edition):
Podany, Michael V, Mr. “Investigation of In-situ Immobilization of Contaminated Sediments using Alginate Gels for the Reduction of Ecological Risk.” 2012. Web. 27 Jan 2021.
Vancouver:
Podany, Michael V M. Investigation of In-situ Immobilization of Contaminated Sediments using Alginate Gels for the Reduction of Ecological Risk. [Internet] [Masters thesis]. University of Connecticut; 2012. [cited 2021 Jan 27].
Available from: https://opencommons.uconn.edu/gs_theses/371.
Council of Science Editors:
Podany, Michael V M. Investigation of In-situ Immobilization of Contaminated Sediments using Alginate Gels for the Reduction of Ecological Risk. [Masters Thesis]. University of Connecticut; 2012. Available from: https://opencommons.uconn.edu/gs_theses/371
Victoria University of Wellington
6.
Ghosh, Sayani.
Characterisation of the Genes Encoding Mannuronan-C5-Epimerase in the Brown Alga Lessonia Variegata.
Degree: 2012, Victoria University of Wellington
URL: http://hdl.handle.net/10063/2654
► Alginate is known to be a commercially valuable polysaccharide, of great importance in industries such as food, cosmetics, medicine and pharmaceuticals. It is obtained commercially…
(more)
▼ Alginate is known to be a commercially valuable polysaccharide, of great importance in
industries such as food, cosmetics, medicine and pharmaceuticals. It is obtained
commercially by harvesting brown algae. The final step in the
alginate biochemical
pathway involves the epimerization of D-mannuronic residues into L-guluronic residues,
catalyzed by the enzyme mannuronan-C5-epimerase. This final step has been found to be
responsible for controlling the physicochemical properties of the produced
alginate. This
study is the first to characterize the genes encoding for the enzyme mannuronan-C5-
epimerase in the Northern, Southern and Wellington lineages of the brown alga Lessonia
variegata (Phaeophyceae). The gene of interest was amplified by standard PCR and
cloning. Cloning PCR results revealed the presence of two distinct copies of the gene in
Lessonia variegata. The coding region of the copies was found to be very conserved with
very little sequence variation. The Lessonia variegata sequences were compared with
those of Laminaria digitata and Saccharina japonica, which indicated that at least one
gene duplication event has occurred in Lessonia variegata, leading to the formation of
two gene duplicates. The possible mechanisms by which the gene paralogs may control
the structure and function of the produced
alginate have been discussed.
Advisors/Committee Members: Zuccarello, Joe.
Subjects/Keywords: Alginate; Gene duplication; Phaeophyceae
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APA (6th Edition):
Ghosh, S. (2012). Characterisation of the Genes Encoding Mannuronan-C5-Epimerase in the Brown Alga Lessonia Variegata. (Masters Thesis). Victoria University of Wellington. Retrieved from http://hdl.handle.net/10063/2654
Chicago Manual of Style (16th Edition):
Ghosh, Sayani. “Characterisation of the Genes Encoding Mannuronan-C5-Epimerase in the Brown Alga Lessonia Variegata.” 2012. Masters Thesis, Victoria University of Wellington. Accessed January 27, 2021.
http://hdl.handle.net/10063/2654.
MLA Handbook (7th Edition):
Ghosh, Sayani. “Characterisation of the Genes Encoding Mannuronan-C5-Epimerase in the Brown Alga Lessonia Variegata.” 2012. Web. 27 Jan 2021.
Vancouver:
Ghosh S. Characterisation of the Genes Encoding Mannuronan-C5-Epimerase in the Brown Alga Lessonia Variegata. [Internet] [Masters thesis]. Victoria University of Wellington; 2012. [cited 2021 Jan 27].
Available from: http://hdl.handle.net/10063/2654.
Council of Science Editors:
Ghosh S. Characterisation of the Genes Encoding Mannuronan-C5-Epimerase in the Brown Alga Lessonia Variegata. [Masters Thesis]. Victoria University of Wellington; 2012. Available from: http://hdl.handle.net/10063/2654
University of Texas – Austin
7.
Mayes, Sarah Margaret.
Hyaluronic acid and alginate blend hydrogel films for the prevention of postsurgical adhesions.
Degree: PhD, Biomedical Engineering, 2013, University of Texas – Austin
URL: http://hdl.handle.net/2152/22058
► Postoperative adhesions form as the body's natural response to injury in an effort to temporarily protect and supply nutrients to these tissues. However, adhesions can…
(more)
▼ Postoperative adhesions form as the body's natural response to injury in an effort to temporarily protect and supply nutrients to these tissues. However, adhesions can remain permanent, and fail otherwise successful surgeries by tethering tissues together that are normally separated. An ideal anti-adhesive device reduces unwanted adhesions and leaves the patient in a state most similar to before surgery. This dissertation introduces a novel, robust hydrogel film consisting of two hydrophilic polydisaccharides, hyaluronic acid (HA) and
alginate. To address the challenge of retaining HA in
alginate-rich hydrogels, we methacrylated the HA (GMHA), creating hydrophobic moieties. These hydrophobic interactions shift the percolation threshold, allowing for greater concentrations of GMHA to be retained in resulting films. UV crosslinking retains GMHA beyond the percolation threshold and widens the possibilities of usable films. To enhance the mechanical properties of these
alginate/GMHA films, we employed a previously developed method for creating thin, branched, interconnected fibers using urea crystal templating. Templated films are softer and, yet, tougher than films that have not been templated. This toughness is a result of increased density of polymer in the fibers. These films were selected as most conformable and most robust by surgeons in a blinded handling study. In a rat peritoneal abrasion model for adhesion formation, the films successfully prevented adhesions with statistical equivalence to the leading anti-adhesion device commercially available. Finally, future recommendations are suggested for the development of a bilayer construct with a collagen/
alginate blend bound to an
alginate/HA layer for an anti-adhesive and regenerative strategy. This construct addresses the need for opposing strategies in the dynamic environment of wound healing. Further research is needed to develop the usefulness of this bilayer system, as preventing unwanted adhesions is merely a first step in achieving a blemish-free healed wound.
Advisors/Committee Members: Stachowiak, Jeanne Casstevens (advisor), Schmidt, Christine E. (advisor).
Subjects/Keywords: Hyaluronic acid; Alginate; Abdominal adhesions
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APA (6th Edition):
Mayes, S. M. (2013). Hyaluronic acid and alginate blend hydrogel films for the prevention of postsurgical adhesions. (Doctoral Dissertation). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/22058
Chicago Manual of Style (16th Edition):
Mayes, Sarah Margaret. “Hyaluronic acid and alginate blend hydrogel films for the prevention of postsurgical adhesions.” 2013. Doctoral Dissertation, University of Texas – Austin. Accessed January 27, 2021.
http://hdl.handle.net/2152/22058.
MLA Handbook (7th Edition):
Mayes, Sarah Margaret. “Hyaluronic acid and alginate blend hydrogel films for the prevention of postsurgical adhesions.” 2013. Web. 27 Jan 2021.
Vancouver:
Mayes SM. Hyaluronic acid and alginate blend hydrogel films for the prevention of postsurgical adhesions. [Internet] [Doctoral dissertation]. University of Texas – Austin; 2013. [cited 2021 Jan 27].
Available from: http://hdl.handle.net/2152/22058.
Council of Science Editors:
Mayes SM. Hyaluronic acid and alginate blend hydrogel films for the prevention of postsurgical adhesions. [Doctoral Dissertation]. University of Texas – Austin; 2013. Available from: http://hdl.handle.net/2152/22058
Duquesne University
8.
Fu, Shao.
Inter-Grade and Inter-Batch Variability of Pharmaceutical-Grade Sodium Alginate.
Degree: PhD, Pharmaceutics, 2011, Duquesne University
URL: https://dsc.duq.edu/etd/557
► Polymeric excipients are generally the least well-characterized components of pharmaceutical formulations. The aim of this dissertation work is to facilitate the quality-by-design (QbD) approach to…
(more)
▼ Polymeric excipients are generally the least well-characterized components of pharmaceutical formulations. The aim of this dissertation work is to facilitate the quality-by-design (QbD) approach to pharmaceutical formulation and manufacturing by evaluating the inter-grade and inter-batch variability of pharmaceutical-grade polymeric excipients. Sodium
alginate, a widely used polymeric excipient, was selected for evaluation using appropriate analytical methods and test conditions, especially rheological methods. The materials used were six different grades of sodium
alginate and an additional ten batches of one of the grades.
To compare the six grades, steady shear measurements were conducted on solutions at 1, 2, and 3% w/w, consistent with their use as thickening or binding agents. Small amplitude oscillation (SAO) measurements were conducted on sodium
alginate solutions at higher concentrations (4-13% w/w) corresponding to their use in controlled release matrices. In order to compare the ten batches of one grade, steady shear and SAO measurements were performed on their solutions at 2% w/w and 8% w/w, respectively. Results show that rheological properties of sodium
alginate solutions are influenced by both molecular weight and chemical composition of sodium
alginate. ¡§One-point¡¨ apparent viscosity data obtained at one low concentration and one shear rate is not representative of the complex rheological behavior of various grades of sodium
alginate solutions at higher concentrations or other shear rates. The potential interchangeability of these different grades used as thickening or binding agents could be established by comparing the apparent viscosities of their solutions as a function of both
alginate concentration and shear conditions. For sodium
alginate used in controlled release formulations, both steady shear (at one low concentration, e.g., 2% w/w) and SAO measurements (at one high concentration indicative of polymer gel state, e.g., 8% w/w) are recommended to be performed on sodium
alginate solutions to ensure interchangeability. Furthermore, among batches of the same grade, significant differences in rheological properties were observed, especially at the high solution concentration (i.e., 8% w/w). In summary, inter-grade and inter-batch variability of sodium
alginate can be determined using steady shear and SAO methods.
The influence of inter-grade and inter-batch variability of sodium
alginate on the functionality of sodium
alginate used in matrix tablets was investigated with a focus on compression properties, swelling, erosion behavior of
alginate matrix tablets, and drug release from matrix tablets. The compression behavior of four grades and three batches of sodium
alginate were studied by compaction energetics, out-of-die Gurnham, and out-of-die Heckel analysis. It was found that sodium alginates deform less plastically than microcrystalline cellulose (MCC PH102) but similar to lactose anhydrous. Sodium alginates also demonstrate more elastic deformations during compression than…
Advisors/Committee Members: Lawrence H. Block, Peter Wildfong, Ira Buckner, Brian Carlin, Sachin Velankar, Christianah Moji Adeyeye.
Subjects/Keywords: Calcium alginate gels; Controlled release matrix; Rheology; Sodium alginate; Viscoelasticity; Viscosity
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APA (6th Edition):
Fu, S. (2011). Inter-Grade and Inter-Batch Variability of Pharmaceutical-Grade Sodium Alginate. (Doctoral Dissertation). Duquesne University. Retrieved from https://dsc.duq.edu/etd/557
Chicago Manual of Style (16th Edition):
Fu, Shao. “Inter-Grade and Inter-Batch Variability of Pharmaceutical-Grade Sodium Alginate.” 2011. Doctoral Dissertation, Duquesne University. Accessed January 27, 2021.
https://dsc.duq.edu/etd/557.
MLA Handbook (7th Edition):
Fu, Shao. “Inter-Grade and Inter-Batch Variability of Pharmaceutical-Grade Sodium Alginate.” 2011. Web. 27 Jan 2021.
Vancouver:
Fu S. Inter-Grade and Inter-Batch Variability of Pharmaceutical-Grade Sodium Alginate. [Internet] [Doctoral dissertation]. Duquesne University; 2011. [cited 2021 Jan 27].
Available from: https://dsc.duq.edu/etd/557.
Council of Science Editors:
Fu S. Inter-Grade and Inter-Batch Variability of Pharmaceutical-Grade Sodium Alginate. [Doctoral Dissertation]. Duquesne University; 2011. Available from: https://dsc.duq.edu/etd/557
University of Maryland
9.
Javvaji, Vishal.
New Concepts for Gelation of Alginate and its Derivatives.
Degree: Chemical Engineering, 2013, University of Maryland
URL: http://hdl.handle.net/1903/14796
► Bioengineering applications require materials that offer tunable and precise control over material properties. In particular, hydrogels of the polysaccharide, alginate have been widely studied for…
(more)
▼ Bioengineering applications require materials that offer tunable and precise control over material properties. In particular, hydrogels of the polysaccharide,
alginate have been widely studied for applications such as drug-delivery vehicles, matrices for encapsulation of cells, and scaffolds for tissue engineering. The ability of
alginate to form a physically cross-linked hydrogel under mild conditions is a key factor for many applications. Traditionally,
alginate gelation has been induced by the addition of divalent ions like calcium (Ca
2+). In this work, we explore new ways to induce gelation of
alginate or its derivatives. These new routes are of interest because they can allow researchers to circumvent current limitations and moreover they can also enable new applications. Three new concepts are explored: (1) ionic gelation activated by light; (2) ionic gelation activated by an enzyme and its substrate; (3) gelation of hydrophobically modified
alginate mediated by biological cells.
In our first study, we demonstrate a concept for ionic gelation of
alginate in response to light, which enables us to create chemically erasable and spatially selective patterns of
alginate gels. We impart light responsiveness by combining
alginate, an insoluble calcium vector (e.g., CaCO
3) and a light responsive component, viz. a photoacid generator (PAG). Upon UV irradiation, the PAG dissociates to release H
+ ions, which react with the CaCO
3 to generate free Ca
2+ in-situ. In turn, the Ca
2+ ions cross-link the
alginate to form a gel. We show photopatterning of
alginate gels, which are used to entrap contents (e.g., microparticles) and subsequently release them by a Ca
2+ chelator.
In our second study, we demonstrate enzymatic gelation of
alginate. Here, we use an enzyme/substrate reaction to generate H
+ ions. The components of our system are glucose oxidase (GOx, enzyme), glucose (substrate),
alginate and CaCO
3. First, GOx catalyzes oxidation of glucose to generate H
+ ions. These H
+ ions solubilize CaCO
3 and release free Ca
2+ ions in-situ. In turn, Ca
2+ ions cross-link
alginate chains into a gel. A sol-gel transition is observed only when GOx senses and catalyzes glucose. By exploiting the specificity of the enzyme for its substrate, we use this concept to build a visual test for the presence of glucose in an unknown product.
In our final study, we induce gels by combining a hydrophobically modified (hm) derivative of
alginate with biological cells. Gelation occurs due to hydrophobic interactions between the grafted hydrophobes and the bilayers of biological cells. The polymer chains thus get attached to the cells and bridge the cells into a three-dimensional network. This gelation can also be reversed (to release the cells) by addition of a supramolecule, α-cyclodextrin, which has a hydrophobic binding pocket that binds to the…
Advisors/Committee Members: Raghavan, Srinivasa R (advisor).
Subjects/Keywords: Chemical engineering; Alginate; enzyme; gelation; hydrophobicall modified alginate; photogelation
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APA (6th Edition):
Javvaji, V. (2013). New Concepts for Gelation of Alginate and its Derivatives. (Thesis). University of Maryland. Retrieved from http://hdl.handle.net/1903/14796
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Javvaji, Vishal. “New Concepts for Gelation of Alginate and its Derivatives.” 2013. Thesis, University of Maryland. Accessed January 27, 2021.
http://hdl.handle.net/1903/14796.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Javvaji, Vishal. “New Concepts for Gelation of Alginate and its Derivatives.” 2013. Web. 27 Jan 2021.
Vancouver:
Javvaji V. New Concepts for Gelation of Alginate and its Derivatives. [Internet] [Thesis]. University of Maryland; 2013. [cited 2021 Jan 27].
Available from: http://hdl.handle.net/1903/14796.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Javvaji V. New Concepts for Gelation of Alginate and its Derivatives. [Thesis]. University of Maryland; 2013. Available from: http://hdl.handle.net/1903/14796
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
10.
Quyen, Nguyen.
Core-Shell Alginate Microgles with an Electro-Responsive Movement.
Degree: Japan Advanced Institute of Science and Technology / 北陸先端科学技術大学院大学
URL: http://hdl.handle.net/10119/8357
Supervisor:Associate Professor TATSUO KANEKO
マテリアルサイエンス研究科
修士
Subjects/Keywords: Alginate; Electro-Responsive
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APA (6th Edition):
Quyen, N. (n.d.). Core-Shell Alginate Microgles with an Electro-Responsive Movement. (Thesis). Japan Advanced Institute of Science and Technology / 北陸先端科学技術大学院大学. Retrieved from http://hdl.handle.net/10119/8357
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Quyen, Nguyen. “Core-Shell Alginate Microgles with an Electro-Responsive Movement.” Thesis, Japan Advanced Institute of Science and Technology / 北陸先端科学技術大学院大学. Accessed January 27, 2021.
http://hdl.handle.net/10119/8357.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Quyen, Nguyen. “Core-Shell Alginate Microgles with an Electro-Responsive Movement.” Web. 27 Jan 2021.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Vancouver:
Quyen N. Core-Shell Alginate Microgles with an Electro-Responsive Movement. [Internet] [Thesis]. Japan Advanced Institute of Science and Technology / 北陸先端科学技術大学院大学; [cited 2021 Jan 27].
Available from: http://hdl.handle.net/10119/8357.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.
Council of Science Editors:
Quyen N. Core-Shell Alginate Microgles with an Electro-Responsive Movement. [Thesis]. Japan Advanced Institute of Science and Technology / 北陸先端科学技術大学院大学; Available from: http://hdl.handle.net/10119/8357
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.
Anna University
11.
Praveena Juliya Dorathi R.
Degradation of chlorophenols by sonication coupled
ferrate and ferrous alginate and dechlorination by iron immobilized
silica;.
Degree: 2014, Anna University
URL: http://shodhganga.inflibnet.ac.in/handle/10603/14691
► Due to industrialization and urbanization, rapid depletion of potable water and pollution of water bodies are taking place. Grossly, polluted water has hindered the ability…
(more)
▼ Due to industrialization and urbanization, rapid
depletion of potable water and pollution of water bodies are taking
place. Grossly, polluted water has hindered the ability of nature
to revive and sustain itself. This scenario had led to the
formation of stringent environmental regulatory laws. Hence,
researchers worldwide over are trying to develop new techniques
which are effective and economical for the degradation of the
recalcitrants. Chlorophenols are mainly used as disinfectants,
biocides, preservatives, pesticides and in the manufacturing of
other chlorinated chemicals. Experiments were conducted using +6,
+2 and 0 oxidation state ofiron to degrade chlorophenols in
innovative ways. The drawback of using ferrate is that it is stable
only at high alkaline pH, requirement of high ferate : chlorophenol
ratio, use of buffers in degradation study (not suitable for waste
water) and storage. Alginate, a polymer obtained from natural
source (brown algae) has been used as support material for ferrous
immobilization in this research. It was coupled with sonication for
better efficiency. Ferrous alginate beads were prepared using a
simple protocol. It was found to be stable at pH 2, which is an
added advantage as the optimal pH for Fenton s method is 2 3. The
order of degradation among chlorophenols was CPgt DCPgt TCP and the
degradation followed pseudo first order kinetics. Immobilization of
Fe0 (ZVI) is also a topic of research for many researchers.
Granular silica, which is easily available, has been experimented
as a support material for ZVI. The indigenously prepared ZVI
immobilized silica was found to be effective in dechlorinating the
chlorophenols. The optimal pH, column height and flow rate was
found to be 7, 20 cm and 0.75L/h respectively.The order of
dechlorination was TCPgt DCPgt CP. All the methods developed in
this study were applied to real pulp and paper industrial
wastewater. The most economically viable and efficient method among
them was sono heterogeneous Fenton method. newline newline
newline
Appendix 1, pp.170-172
Advisors/Committee Members: Palanivelu, K..
Subjects/Keywords: Chlorophenols; dechlorination; ferrous alginate;
immobilized silica
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APA ·
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Export
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Manager
APA (6th Edition):
R, P. J. D. (2014). Degradation of chlorophenols by sonication coupled
ferrate and ferrous alginate and dechlorination by iron immobilized
silica;. (Thesis). Anna University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/14691
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
R, Praveena Juliya Dorathi. “Degradation of chlorophenols by sonication coupled
ferrate and ferrous alginate and dechlorination by iron immobilized
silica;.” 2014. Thesis, Anna University. Accessed January 27, 2021.
http://shodhganga.inflibnet.ac.in/handle/10603/14691.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
R, Praveena Juliya Dorathi. “Degradation of chlorophenols by sonication coupled
ferrate and ferrous alginate and dechlorination by iron immobilized
silica;.” 2014. Web. 27 Jan 2021.
Vancouver:
R PJD. Degradation of chlorophenols by sonication coupled
ferrate and ferrous alginate and dechlorination by iron immobilized
silica;. [Internet] [Thesis]. Anna University; 2014. [cited 2021 Jan 27].
Available from: http://shodhganga.inflibnet.ac.in/handle/10603/14691.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
R PJD. Degradation of chlorophenols by sonication coupled
ferrate and ferrous alginate and dechlorination by iron immobilized
silica;. [Thesis]. Anna University; 2014. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/14691
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Penn State University
12.
Chandrasekar, Vaishnavi.
Release kinetics of nisin from chitosan-alginate films and microparticles
.
Degree: 2015, Penn State University
URL: https://submit-etda.libraries.psu.edu/catalog/26302
► Antimicrobials are being used to inhibit the growth of pathogenic and spoilage bacteria, thereby enhancing safety and extending the shelf-life of processed foods. Nisin is…
(more)
▼ Antimicrobials are being used to inhibit the growth of pathogenic and spoilage bacteria, thereby enhancing safety and extending the shelf-life of processed foods. Nisin is a bacteriocin that has Generally Recognized as Safe (GRAS) status, and is effective against Gram positive bacteria. Studies have shown that when nisin is directly applied to the surface of foods, it loses its biological activity over time. This loss is due to binding interactions with proteins and fats, and degradation by proteolytic enzymes, as well as due to diffusion into the bulk of the food. Delivery systems with sustained release properties of nisin have the potential to improve its stability and biological functionality in foods. Chitosan-
alginate polyelectric complexes are an effective drug delivery tool used in the pharmaceutical industry for controlled release of antimicrobials. These materials can be used for controlled release of nisin as a film or microparticle system. The goal of this research was to develop and characterize chitosan-
alginate films and microparticles containing nisin, and understand the release kinetics of nisin in an aqueous system. The specific objectives were (1) to determine the factors affecting release of nisin from chitosan-
alginate films, (2) to determine the release kinetics of nisin from chitosan-
alginate films and study component interactions, and (3) to evaluate the release kinetics of nisin from chitosan-
alginate microparticles.
The effects of polymer concentration (0.5 and 1.0%), chitosan molecular weight (low and high) and ratio of chitosan to
alginate (expressed as 33, 50 and 66%
alginate fraction) were investigated on the release of nisin from chitosan-
alginate films. The polymer concentration and
alginate fraction were found to have a significant effect (p<0.05) on nisin release. Highest nisin release was obtained for films prepared using 0.5% polymer concentration and 33%
alginate fraction. SEM images indicated that the ratio of chitosan to
alginate influenced the film microstructure.
The release kinetics of nisin from chitosan-
alginate films prepared using various
alginate fractions (33, 50 and 66%) was investigated using a two-temperature agar diffusion bioassay. The total amount of nisin released from films into an aqueous system decreased significantly (p<0.05) with an increase in
alginate concentration. The mechanism of diffusion of nisin from all films was found to be Fickian, and the diffusion coefficients varied from 0.87 x 10-9 to 8.03 x 10-9 cm2/s. Strong complexation was confirmed between chitosan and
alginate polymers within the films, particularly at high
alginate fractions. Binding between nisin and
alginate was also confirmed, resulting in reduction of freely available nisin within films.
Chitosan-
alginate microparticles containing nisin, were produced by preparing a calcium-
alginate pre-gel, followed by chitosan complexation. Calcium concentrations ranging from 0.25 to 2 mM were found to be effective in inducing the formation of a coiled
alginate nucleus. Particles obtained were in…
Advisors/Committee Members: Ramaswamy C Anantheswaran, Dissertation Advisor/Co-Advisor, John Neil Coupland, Committee Member, Catherine Nettles Cutter, Committee Member, Ali Demirci, Committee Member.
Subjects/Keywords: nisin; release; diffusion; chitosan-alginate; films; microparticles
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APA (6th Edition):
Chandrasekar, V. (2015). Release kinetics of nisin from chitosan-alginate films and microparticles
. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/26302
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Chandrasekar, Vaishnavi. “Release kinetics of nisin from chitosan-alginate films and microparticles
.” 2015. Thesis, Penn State University. Accessed January 27, 2021.
https://submit-etda.libraries.psu.edu/catalog/26302.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Chandrasekar, Vaishnavi. “Release kinetics of nisin from chitosan-alginate films and microparticles
.” 2015. Web. 27 Jan 2021.
Vancouver:
Chandrasekar V. Release kinetics of nisin from chitosan-alginate films and microparticles
. [Internet] [Thesis]. Penn State University; 2015. [cited 2021 Jan 27].
Available from: https://submit-etda.libraries.psu.edu/catalog/26302.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Chandrasekar V. Release kinetics of nisin from chitosan-alginate films and microparticles
. [Thesis]. Penn State University; 2015. Available from: https://submit-etda.libraries.psu.edu/catalog/26302
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Penn State University
13.
Cho, Seung Wook.
HYDROGEL STABILIZED RESPONSIVE COMPLEX EMULSIONS.
Degree: 2018, Penn State University
URL: https://submit-etda.libraries.psu.edu/catalog/15015szc5433
► Emulsions, which are mixtures of immiscible fluids, have found widespread interest and use in fields and applications ranging from medicine, food, and cosmetics to optics.…
(more)
▼ Emulsions, which are mixtures of immiscible fluids, have found widespread interest and use in fields and applications ranging from medicine, food, and cosmetics to optics. Complex emulsions, in which the droplets contain multiple fluids, are especially exciting due to the correlation between droplet structure and properties. Recently, a method to reconfigure the morphology of complex droplets containing hydrocarbon and fluorocarbon oils in water was demonstrated.1 Such droplets display unique physical and chemical properties rendering them applicable in areas such as sensors and tunable optics. However, emulsions are inherently not stable for extended time periods resulting in a critical obstacle for various applications. Traditional encapsulation approaches that are useful for droplet stabilization would render these hydrocarbon and fluorocarbon droplets non-responsive, thereby negating their most advantageous properties. In this project we aim to address this stability issue by fabricating a thin spherical hydrogel shell around droplets as a barrier to prevent coalescence while simultaneously retaining the droplets’ responsive behavior.
Hydrogels are interesting materials to explore as emulsion stabilizers because they can be both interfacially active and chemically and mechanically tunable. Herein, we will create hydrogel shells composed of both ionically and covalently crosslinked
alginate. In this work, we will explore the effects of a spherical hydrogel shell encapsulating the complex emulsion droplet on the droplet’s responsive behavior. We delve into the hydrogel interfacial behavior, how the droplets respond to the hydrogel and small molecule surfactants in combination, and characterize the hydrogel shell using confocal microscopy and optical microscopy.
Advisors/Committee Members: Lauren Dell Zarzar, Thesis Advisor/Co-Advisor, James Hansell Adair, Committee Member, Robert John Hickey III, Committee Member, Michael Anthony Hickner, Committee Member.
Subjects/Keywords: encapsulation; emulsions; complex emulsions; calcium alginate
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APA ·
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Export
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APA (6th Edition):
Cho, S. W. (2018). HYDROGEL STABILIZED RESPONSIVE COMPLEX EMULSIONS. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/15015szc5433
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Cho, Seung Wook. “HYDROGEL STABILIZED RESPONSIVE COMPLEX EMULSIONS.” 2018. Thesis, Penn State University. Accessed January 27, 2021.
https://submit-etda.libraries.psu.edu/catalog/15015szc5433.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Cho, Seung Wook. “HYDROGEL STABILIZED RESPONSIVE COMPLEX EMULSIONS.” 2018. Web. 27 Jan 2021.
Vancouver:
Cho SW. HYDROGEL STABILIZED RESPONSIVE COMPLEX EMULSIONS. [Internet] [Thesis]. Penn State University; 2018. [cited 2021 Jan 27].
Available from: https://submit-etda.libraries.psu.edu/catalog/15015szc5433.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Cho SW. HYDROGEL STABILIZED RESPONSIVE COMPLEX EMULSIONS. [Thesis]. Penn State University; 2018. Available from: https://submit-etda.libraries.psu.edu/catalog/15015szc5433
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Queens University
14.
Kapishon, Vitaliy.
Modification of Alginate and Development of Polymeric Nanocarriers by Living Radical Polymerization for Controlled/Targeted Drug Delivery
.
Degree: Chemical Engineering, 2015, Queens University
URL: http://hdl.handle.net/1974/13856
► Alginate-based amphiphilic graft copolymers were synthesized by single electron transfer living radical polymerization (SET-LRP), forming stable micelles during polymerization induced self-assembly (PISA). First, alginate macroinitiator…
(more)
▼ Alginate-based amphiphilic graft copolymers were synthesized by single electron transfer living radical polymerization (SET-LRP), forming stable micelles during polymerization induced self-assembly (PISA). First, alginate macroinitiator was prepared by partial depolymerization of native alginate, solubility modification and attachment of initiator. Then, methyl methacrylate was polymerized from the alginate macroinitiator in mixtures of water and methanol forming poly(methyl methacrylate) grafts, prior to self-assembly. PISA of the amphiphilic graft-copolymer resulted in the formation of micelles with diameters of 50-300 nm. As the first reported case of LRP from alginate, this work introduces a synthetic route to of alginate-based hybrid polymers with a precise macromolecular architecture and desired functionalities. LRP from alginate can be applied to improve alginate-based hydrogel systems such as nano- and micro-hydrogel particles, islet encapsulation materials, hydrogel implants and topical applications. Such modified alginates can also improve the function and application of native alginates in food and agricultural technologies.
Targeted drug delivery using polymeric nanostructures has been at the forefront of cancer research engineered for safer, more efficient and effective use of chemotherapy. Here, a new polymeric micelle delivery system was designed for active tumor targeting via receptor-induced endocytosis. Oseltamivir phosphate targets and inhibits Neu1 sialidase activity associated with receptor tyrosine kinases such as epidermal growth factor (EGF) receptors which are overexpressed on cancer cells. By decorating micelles with oseltamivir, it was found that micelles actively targeted human pancreatic PANC1 cancer cells. Amphiphilic block copolymers with oseltamivir conjugated at the hydrophilic end, oseltamivir-poly(polyethylene glycol methyl ether methacrylate)-block-poly(methyl methacrylate), were synthesized using reversible addition-fragmentation chain transfer (RAFT) living radical polymerization. Oseltamivir-conjugated micelles have self-assembling properties forming worm-like micellar structures. Oseltamivir-conjugated micelles dose dependently reduced the cell viability of PANC1 cells compared to micelles without oseltamivir. Oseltamivir-micelles loaded with hydrophobic dye produced internalization by PANC1. These results clearly indicate that oseltamivir-conjugated micelles show potential as an entirely new active tumor targeting drug delivery system with the ability to internalize hydrophobic chemotherapeutics by receptor-mediated endocytosis.
Subjects/Keywords: Living Radical Polymerization
;
Drug Delivery
;
Alginate
;
Biomaterials
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APA ·
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CSE |
Export
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Manager
APA (6th Edition):
Kapishon, V. (2015). Modification of Alginate and Development of Polymeric Nanocarriers by Living Radical Polymerization for Controlled/Targeted Drug Delivery
. (Thesis). Queens University. Retrieved from http://hdl.handle.net/1974/13856
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Kapishon, Vitaliy. “Modification of Alginate and Development of Polymeric Nanocarriers by Living Radical Polymerization for Controlled/Targeted Drug Delivery
.” 2015. Thesis, Queens University. Accessed January 27, 2021.
http://hdl.handle.net/1974/13856.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Kapishon, Vitaliy. “Modification of Alginate and Development of Polymeric Nanocarriers by Living Radical Polymerization for Controlled/Targeted Drug Delivery
.” 2015. Web. 27 Jan 2021.
Vancouver:
Kapishon V. Modification of Alginate and Development of Polymeric Nanocarriers by Living Radical Polymerization for Controlled/Targeted Drug Delivery
. [Internet] [Thesis]. Queens University; 2015. [cited 2021 Jan 27].
Available from: http://hdl.handle.net/1974/13856.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Kapishon V. Modification of Alginate and Development of Polymeric Nanocarriers by Living Radical Polymerization for Controlled/Targeted Drug Delivery
. [Thesis]. Queens University; 2015. Available from: http://hdl.handle.net/1974/13856
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Toronto
15.
Wong, Steven.
Structure and Role of P. aeruginosa AlgL in Alginate Biosynthesis.
Degree: 2016, University of Toronto
URL: http://hdl.handle.net/1807/92619
► During chronic infection, Pseudomonas aeruginosa generate alginate-containing biofilms in the lungs of cystic fibrosis patients. Mature bacterial alginate is a heteropolymer of Îą-L-guluronic acid and…
(more)
▼ During chronic infection, Pseudomonas aeruginosa generate alginate-containing biofilms in the lungs of cystic fibrosis patients. Mature bacterial alginate is a heteropolymer of Îą-L-guluronic acid and partially acetylated β-D-mannuronic acid. P. aeruginosa alginate biosynthesis requires thirteen proteins, including an alginate lyase, AlgLPa, which has been proposed to have a bi-functional role, both as a lyase and as an integral part of the cell envelope biosynthetic complex. How AlgLPa performs these functions is currently poorly understood. We have used an in vitro structure-function approach to explore the substrate recognition and catalytic mechanism of AlgLPa, and in vivo complementation to explore the role of its activity in alginate biosynthesis. The results presented suggest that AlgLPa is an endo-acting lyase with at least six subsites, and that the loop containing K66 is critical for substrate binding and/or catalysis. The complementation data supports the hypothesis that AlgLPa assembles into the cell envelope biosynthetic complex.
M.Sc.
2018-11-30 00:00:00
Advisors/Committee Members: Howell, P. Lynne, Biochemistry.
Subjects/Keywords: Alginate; Bacteria; Biofilm; Infection; Lyase; Polysaccharide; 0487
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APA (6th Edition):
Wong, S. (2016). Structure and Role of P. aeruginosa AlgL in Alginate Biosynthesis. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/92619
Chicago Manual of Style (16th Edition):
Wong, Steven. “Structure and Role of P. aeruginosa AlgL in Alginate Biosynthesis.” 2016. Masters Thesis, University of Toronto. Accessed January 27, 2021.
http://hdl.handle.net/1807/92619.
MLA Handbook (7th Edition):
Wong, Steven. “Structure and Role of P. aeruginosa AlgL in Alginate Biosynthesis.” 2016. Web. 27 Jan 2021.
Vancouver:
Wong S. Structure and Role of P. aeruginosa AlgL in Alginate Biosynthesis. [Internet] [Masters thesis]. University of Toronto; 2016. [cited 2021 Jan 27].
Available from: http://hdl.handle.net/1807/92619.
Council of Science Editors:
Wong S. Structure and Role of P. aeruginosa AlgL in Alginate Biosynthesis. [Masters Thesis]. University of Toronto; 2016. Available from: http://hdl.handle.net/1807/92619
University of Ottawa
16.
Parsian, Ava.
Simvastatin Encapsulation in Alginate-Based Microspheres
.
Degree: 2016, University of Ottawa
URL: http://hdl.handle.net/10393/34439
► Despite the great success of hip implant surgeries, wear particle-induced implant aseptic loosening still limits the implant longevity. Simvastatin, an FDA-approved cholesterol lowering statin, is…
(more)
▼ Despite the great success of hip implant surgeries, wear particle-induced implant aseptic loosening still limits the implant longevity. Simvastatin, an FDA-approved cholesterol lowering statin, is a promising drug candidate for the treatment of implant aseptic loosening due to its anti-inflammatory properties as well as its ability to stimulate bone growth and inhibit bone resorption. In addition, alginate microspheres have been used extensively in drug delivery applications because of alginate properties, including biocompatibility and gelation in mild conditions. However, the hydrophobicity of simvastatin, as well as the large alginate microsphere pore size leading to the leakage of low molecular weight drugs are limiting factors for their use as a delivery system for simvastatin. Therefore, the objectives of this thesis were twofold: 1. To complex simvastatin with 2-hydroxypropyl-β-cyclodextrin (HP-βCD) in order to increase its solubility; and 2. To increase simvastatin encapsulation efficiency in alginate microspheres by coating the microspheres with chitosan, adding dextran sulfate in the alginate solution, and optimizing the gelation conditions used for the synthesis of the microspheres (e.g., volume of gelation medium, curing time, and addition of simvastatin in the gelation medium). Results showed that simvastatin complexation with HP-βCD increased with HP-βCD to simvastatin molar ratio, to a maximum of 97.6% at the molar ratio of 10. Results also showed that chitosan coating of the alginate microspheres increased simvastatin encapsulation efficiency (up to 10.6%), which was further improved (up to 14.0%) when adding 2.0% (w/v) dextran sulfate to the alginate solution. This increase was likely due to electrostatic interactions between dextran sulfate and chitosan in addition to alginate, resulting in a denser coating. Finally, the addition of simvastatin in the gelation medium was shown to also increase simvastatin encapsulation (up to 22.4%), likely because of a decrease in the diffusion of simvastatin out of the microspheres. Overall, this work completed the initial steps for the development of an alginate-based drug delivery system for simvastatin with the long-term goal of providing a local delivery of simvastatin to modulate implant aseptic loosening.
Subjects/Keywords: Drug Delivery System;
Alginate;
Microspheres;
Encapsulation;
Simvastatin
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Parsian, A. (2016). Simvastatin Encapsulation in Alginate-Based Microspheres
. (Thesis). University of Ottawa. Retrieved from http://hdl.handle.net/10393/34439
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Parsian, Ava. “Simvastatin Encapsulation in Alginate-Based Microspheres
.” 2016. Thesis, University of Ottawa. Accessed January 27, 2021.
http://hdl.handle.net/10393/34439.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Parsian, Ava. “Simvastatin Encapsulation in Alginate-Based Microspheres
.” 2016. Web. 27 Jan 2021.
Vancouver:
Parsian A. Simvastatin Encapsulation in Alginate-Based Microspheres
. [Internet] [Thesis]. University of Ottawa; 2016. [cited 2021 Jan 27].
Available from: http://hdl.handle.net/10393/34439.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Parsian A. Simvastatin Encapsulation in Alginate-Based Microspheres
. [Thesis]. University of Ottawa; 2016. Available from: http://hdl.handle.net/10393/34439
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Univerzitet u Beogradu
17.
Prodanović, Olivera L. 1973-.
Razvoj imobilisanih sistema peroksidaze iz rena
(Armoracia rusticana) za polimerizacione reakcije i uklanjanje
fenola iz otpadnih voda.
Degree: Tehnološko-metalurški fakultet, 2016, Univerzitet u Beogradu
URL: https://fedorabg.bg.ac.rs/fedora/get/o:11259/bdef:Content/get
► Biotehnologija i biohemijsko inženjerstvo / Biotechnology and Biochemical Engineering
U prvom delu ove teze je makroporozni kopolimer glicidil metakrilata i etilen glikol dimetakrilata, poly(GMA-co-EGDMA) sa…
(more)
▼ Biotehnologija i biohemijsko inženjerstvo /
Biotechnology and Biochemical Engineering
U prvom delu ove teze je makroporozni kopolimer
glicidil metakrilata i etilen glikol dimetakrilata,
poly(GMA-co-EGDMA) sa dijametrom čestica od 150 do 500 μm i
različitim karakteristikama površine i različitom srednjom
veličinom dijametra pora od 44 do 200 nm sintetisan, modifikovan
1,2-diaminoetanom i testiran kao nosač za imobilizaciju peroksidaze
iz rena (HRP) koristeći dve kovalentne metode, glutaraldehidnu i
perjodatnu. Najveća specifična aktivnost od oko 35 U/g suve mase
nosača je postignuta na poly(GMA-co-EGDMA) kopolimeru sa srednjom
veličinom dijametra pora od 200 i 120 nm koristeći perjodatni
metod. Proučavanjem kinetike deaktivacije na 65 °C i u 80% (v/v)
dioksanu je otkriveno da perjodatna metoda takođe dovodi do
značajne stabilizacije biokatalizatora, i da stabilizacioni faktor
zavisi snažno od površinskih karakteristika kopolimera. HRP
imobilizovana na kopolimeru sa srednjom veličinom dijametra pora od
120 nm perjodatnom metodom je pokazala najveću specifičnu aktivnost
ali takođe i dobru stabilnost, tako da je u daljem radu dodatno
karakterisana. Imobilizacija je dovela do stabilizacije enzima u
širem opsegu pH, dok je Mihaelisova konstanta (Km) imobilizovane
HRP bila 10,8 mM što je otprilike 5,6 puta više nego za slobodni
enzim. Posle 6 upotreba u bač reaktoru za oksidaciju pirogalola
imobilizovana peroksidaza je zadržala 45% polazne aktivnosti. U
drugom delu ove teze ista serija makroporoznih kopolimera glicidil
metakrilata i etilen glikol dimetakrilata, poly(GMA-co-EGDMA) sa
veličinom pora od 200 nm (ZS9) pa do neporoznog (ZS6) je testirana
kao nosač za imobilizaciju peroksidaze iz rena, ali ovaj put sa
dijametrom čestica od 2 do 2,5 μm. HRP je imobilizovana na 4
načina, preko epoksidne grupe na kopolimeru, i nakon aminovanja
kopolimera glutaraldehidnom, perjodatnom i karbodiimidnom metodom.
Najveća aktivnost imobilizovane HRP od 14,5 U/g je dobijena
glutaraldehidnom metodom, a odmah zatim najveća aktivnost od 13,7
U/g karbodiimidnom metodom na poly(GMA-co-EGDMA) označenom kao ZS9
čija je srednja veličina pora bila oko 200 nm. Prinos imobilizacije
je bio veći u odnosu na seriju kopolimera sa većom veličinom
čestica i iznosio je 33,5% za glutaraldehidnu metodu i 30,3 % za
karbodiimidnu u odnosu na oko 5% prinosa dobijenog za seriju
kopolimera sa većom veličinom čestica i...
Advisors/Committee Members: Knežević-Jugović, Zorica..
Subjects/Keywords: peroxidase; macroporous; alginate; emulsion; tyramine;
phenol
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Prodanović, O. L. 1. (2016). Razvoj imobilisanih sistema peroksidaze iz rena
(Armoracia rusticana) za polimerizacione reakcije i uklanjanje
fenola iz otpadnih voda. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:11259/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Prodanović, Olivera L 1973-. “Razvoj imobilisanih sistema peroksidaze iz rena
(Armoracia rusticana) za polimerizacione reakcije i uklanjanje
fenola iz otpadnih voda.” 2016. Thesis, Univerzitet u Beogradu. Accessed January 27, 2021.
https://fedorabg.bg.ac.rs/fedora/get/o:11259/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Prodanović, Olivera L 1973-. “Razvoj imobilisanih sistema peroksidaze iz rena
(Armoracia rusticana) za polimerizacione reakcije i uklanjanje
fenola iz otpadnih voda.” 2016. Web. 27 Jan 2021.
Vancouver:
Prodanović OL1. Razvoj imobilisanih sistema peroksidaze iz rena
(Armoracia rusticana) za polimerizacione reakcije i uklanjanje
fenola iz otpadnih voda. [Internet] [Thesis]. Univerzitet u Beogradu; 2016. [cited 2021 Jan 27].
Available from: https://fedorabg.bg.ac.rs/fedora/get/o:11259/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Prodanović OL1. Razvoj imobilisanih sistema peroksidaze iz rena
(Armoracia rusticana) za polimerizacione reakcije i uklanjanje
fenola iz otpadnih voda. [Thesis]. Univerzitet u Beogradu; 2016. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:11259/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Univerzitet u Beogradu
18.
Malagurski, Ivana S., 1982-.
Dobijanje i karakterizacija nemineralizovanih i
mineralizovanih biomaterijala na bazi polisaharida morskih algi i
esencijalnog metala cinka.
Degree: Tehnološko-metalurški fakultet, 2018, Univerzitet u Beogradu
URL: https://fedorabg.bg.ac.rs/fedora/get/o:17314/bdef:Content/get
► Tehnološko inženjerstvo - Biohemijsko inženjerstvo i biotehnologija / Technological engineering - Biochemical engineering and biotechnology
U ovoj tezi je ispitana mogućnost sinteze novih, poboljšanih biomaterijala…
(more)
▼ Tehnološko inženjerstvo - Biohemijsko inženjerstvo
i biotehnologija / Technological engineering - Biochemical
engineering and biotechnology
U ovoj tezi je ispitana mogućnost sinteze novih,
poboljšanih biomaterijala na bazi alginata, agara i cinka, primenom
biomimetičkog principa dizajna – biomineralizacije. Cilj
istraživanja je bio dizajn i karakterizacija novih nanokompozitnih
biomaterijala, sa potencijalnom primenom u biomedicini i pakovanju
hrane...
Advisors/Committee Members: Dimitrijević-Branković, Suzana.
Subjects/Keywords: alginate; agar; zinc; nanocomposites;
biomineralization; antimicrobial activity
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Malagurski, Ivana S., 1. (2018). Dobijanje i karakterizacija nemineralizovanih i
mineralizovanih biomaterijala na bazi polisaharida morskih algi i
esencijalnog metala cinka. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:17314/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Malagurski, Ivana S., 1982-. “Dobijanje i karakterizacija nemineralizovanih i
mineralizovanih biomaterijala na bazi polisaharida morskih algi i
esencijalnog metala cinka.” 2018. Thesis, Univerzitet u Beogradu. Accessed January 27, 2021.
https://fedorabg.bg.ac.rs/fedora/get/o:17314/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Malagurski, Ivana S., 1982-. “Dobijanje i karakterizacija nemineralizovanih i
mineralizovanih biomaterijala na bazi polisaharida morskih algi i
esencijalnog metala cinka.” 2018. Web. 27 Jan 2021.
Vancouver:
Malagurski, Ivana S. 1. Dobijanje i karakterizacija nemineralizovanih i
mineralizovanih biomaterijala na bazi polisaharida morskih algi i
esencijalnog metala cinka. [Internet] [Thesis]. Univerzitet u Beogradu; 2018. [cited 2021 Jan 27].
Available from: https://fedorabg.bg.ac.rs/fedora/get/o:17314/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Malagurski, Ivana S. 1. Dobijanje i karakterizacija nemineralizovanih i
mineralizovanih biomaterijala na bazi polisaharida morskih algi i
esencijalnog metala cinka. [Thesis]. Univerzitet u Beogradu; 2018. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:17314/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Univerzitet u Beogradu
19.
Lević, Steva M., 1981-.
Inkapsulacija aroma u karnauba vosku, alginatu i
polivinil-alkoholu.
Degree: Poljoprivredni fakultet, 2016, Univerzitet u Beogradu
URL: https://fedorabg.bg.ac.rs/fedora/get/o:11100/bdef:Content/get
► Biotehničke nauke - Nauka o konzervisanju / Biotechnical sciences - The science of food preservation
U ovoj disertaciji je ispitivana inkapsulacija prehrambenih aroma uz primenu…
(more)
▼ Biotehničke nauke - Nauka o konzervisanju /
Biotechnical sciences - The science of food
preservation
U ovoj disertaciji je ispitivana inkapsulacija
prehrambenih aroma uz primenu različitih metoda inkapsulacije i u
kombinaciji sa prirodnim i sintetičkim nosačima. Cilj istraživanja
je bio dobijanje inkapsulata kako tečnih tako i čvrstih aroma koje
se najčešće koriste u prehrambenoj industriji. Ispitivana su tri
nosača za inkapsulaciju aroma: karnauba vosak, alginat i polivinil
alkohol. Kao model arome su korišćene: etil vanilin, komercijalno
dostupna aroma kokosa i D-limonen. Karnauba vosak je korišćen kao
nosač za inkapsulaciju etil vanilina, pri čemu su dobijene sferne
čestice čiji je prečnik uglavnom bio ispod 250μm. Čestice na bazi
karnauba voska sa etil vanilinom su dobijene primenom disperzione
(emulzifikacione) metode i disperzione tehnike (disperzija
komprimovanim vazduhom). Metodom disperzije vazduhom su dobijene
čestice pravilnijeg sfernog oblika sa većom inkapsulacionom
efikasnošću. Takođe, termička svojstva inkapsulisanog etil vanilina
su poboljšana u poređenju sa slobodnom aromom. Termičko otpuštanje
inkapsulisanog etil vanilina se odigravalo na višim temperaturama
(do~270°C) u poređenju sa slobonom aromom (do ~220°C). Ca-alginatne
čestice sa tečnim i čvrstim aromama su dobijane metodom
elektrostatičke ekstruzije. Inkapsulacijom etil vanilina u
Ca-alginatni matriks su ostvarena bolja termička svojstva
inkapsulisane arome uz postizanje kontrolisanog termičkog
otpuštanja arome u širem temperaturnom opsegu u odnosu na slobodnu
aromu. Aroma kokosa i D-limonen su inkapsulisani u Ca-alginatne
čestice primenom iste procedure kao i u slučaju inkapsulacije etil
vanilina. Alginat je pokazao dobra emulgujuća svojstva, što je
osnovni uslov za postizanje visoke inkapsulacione efikasnosti.
Rezultati termičke analize su pokazali da obe arome u
inkapsulisanom obliku imaju bolja termička svojstva u odnosu na
slobodne arome. Rehidracija je korišćena za ispitivanje adsorpcije
vode u inkapsulate uz razvoj novog modela rehidracije. Polivinil
alkohol je u kombinaciji sa alginatom korišćen za dobijanje čestica
sa inkapsulisanim etil vanilinom metodom elektrostatičke
ekstruzije. Primenom viših napona kod metode elektrostatičke
ekstruzije-elektrospininga dobijeni su filmovi na bazi polivinil
alkohola koji se sastoje od nanovlakana. Filmovi na bazi polivinil
alkohola su pokazali dobra hemijska, mehanička, termička i
antimikrobna svojstva...
Advisors/Committee Members: Nedović, Viktor, 1964-.
Subjects/Keywords: encapsulation; carnauba wax; alginate; polyvinyl
alcohol; flavours
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Record Details
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Lević, Steva M., 1. (2016). Inkapsulacija aroma u karnauba vosku, alginatu i
polivinil-alkoholu. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:11100/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Lević, Steva M., 1981-. “Inkapsulacija aroma u karnauba vosku, alginatu i
polivinil-alkoholu.” 2016. Thesis, Univerzitet u Beogradu. Accessed January 27, 2021.
https://fedorabg.bg.ac.rs/fedora/get/o:11100/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Lević, Steva M., 1981-. “Inkapsulacija aroma u karnauba vosku, alginatu i
polivinil-alkoholu.” 2016. Web. 27 Jan 2021.
Vancouver:
Lević, Steva M. 1. Inkapsulacija aroma u karnauba vosku, alginatu i
polivinil-alkoholu. [Internet] [Thesis]. Univerzitet u Beogradu; 2016. [cited 2021 Jan 27].
Available from: https://fedorabg.bg.ac.rs/fedora/get/o:11100/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Lević, Steva M. 1. Inkapsulacija aroma u karnauba vosku, alginatu i
polivinil-alkoholu. [Thesis]. Univerzitet u Beogradu; 2016. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:11100/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Delft University of Technology
20.
Shetty, Santhosh (author).
Feasibility Study of Alginate Polymer Electrolytes through Molecular Dynamics Simulations.
Degree: 2018, Delft University of Technology
URL: http://resolver.tudelft.nl/uuid:6d0ee194-ec3e-4cac-a162-ff3d56587a22
► Large scale stationary energy storage is becoming the need of the hour as the world transitions to a renewable economy. For this, there is a…
(more)
▼ Large scale stationary energy storage is becoming the need of the hour as the world transitions to a renewable economy. For this, there is a necessity of higher energy density batteries that can cope well both in storing excess energy and minimizing fluctuations on the grid. Solid polymer electrolyte batteries can be extremely safe and reduce packaging while also preventing dendrite formation in Lithium-ion batteries. Sodium ion movement is seen from one oxygen atom to another for an arbitrary Na+ ion. This thesis shows that MD simulations can be a promising method to study the mechanisms involved in
alginate polymer electrolytes. Further work is necessary to enable rigorous analysis by incorporating both mannuronates and guluronates. Neutron Magnetic Resonance (NMR) can be carried out to validate the results obtained through MD simulations. MD simulations on sodium
alginate (primarily of guluronate) as a solid polymer electrolyte appear to indicate the interaction of Na+ ion with O2 atom of the polyguluronate residue is in preference to interaction of Na+ ion with carboxylate oxygen atoms. Diffusion constant of Na+ ion is seen to drop in MD simulations with increase in Ca2+ ion concentrations both at temperatures 300 K and 373 K.
Advisors/Committee Members: Kelder, Erik (mentor), Wagemaker, Marnix (graduation committee), Picken, Stephen (graduation committee), Basak, Shibabrata (mentor), Delft University of Technology (degree granting institution).
Subjects/Keywords: Solid Polymer Electrolyte; Molecular Dynamics; Diffusion; Alginate
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Shetty, S. (. (2018). Feasibility Study of Alginate Polymer Electrolytes through Molecular Dynamics Simulations. (Masters Thesis). Delft University of Technology. Retrieved from http://resolver.tudelft.nl/uuid:6d0ee194-ec3e-4cac-a162-ff3d56587a22
Chicago Manual of Style (16th Edition):
Shetty, Santhosh (author). “Feasibility Study of Alginate Polymer Electrolytes through Molecular Dynamics Simulations.” 2018. Masters Thesis, Delft University of Technology. Accessed January 27, 2021.
http://resolver.tudelft.nl/uuid:6d0ee194-ec3e-4cac-a162-ff3d56587a22.
MLA Handbook (7th Edition):
Shetty, Santhosh (author). “Feasibility Study of Alginate Polymer Electrolytes through Molecular Dynamics Simulations.” 2018. Web. 27 Jan 2021.
Vancouver:
Shetty S(. Feasibility Study of Alginate Polymer Electrolytes through Molecular Dynamics Simulations. [Internet] [Masters thesis]. Delft University of Technology; 2018. [cited 2021 Jan 27].
Available from: http://resolver.tudelft.nl/uuid:6d0ee194-ec3e-4cac-a162-ff3d56587a22.
Council of Science Editors:
Shetty S(. Feasibility Study of Alginate Polymer Electrolytes through Molecular Dynamics Simulations. [Masters Thesis]. Delft University of Technology; 2018. Available from: http://resolver.tudelft.nl/uuid:6d0ee194-ec3e-4cac-a162-ff3d56587a22
Virginia Tech
21.
Pawar, Siddhesh Nitin.
Chemical Modification of Alginates in Organic Media.
Degree: PhD, Macromolecular Science and Engineering, 2013, Virginia Tech
URL: http://hdl.handle.net/10919/52635
► Alginates are (1and4) linked linear copolysaccharides composed of B-D-mannuronic acid (M) and its C-5 epimer, a-L-guluronic acid (G). Several strategies to synthesize organically modified alginate…
(more)
▼ Alginates are (1and4) linked linear copolysaccharides composed of B-D-mannuronic acid (M) and its C-5 epimer, a-L-guluronic acid (G). Several strategies to synthesize organically modified
alginate derivatives have been reported, but almost all chemistries are performed in either aqueous or aqueous-organic media. The ability to react alginates homogeneously in organic solvents would open up access to a wide range of new chemistries and derivatives. However, past attempts have been restricted by the absence of methods for
alginate dissolution in organic media. We therefore report a strategy to solubilize tetrabutylammonium (TBA) salts of alginic acid in polar aprotic solvents containing tetrabutylammonium fluoride (TBAF).
Acylation of TBA-
alginate was performed in DMSO/TBAF to get products with DSacetyl up to ~ 1.0. We further report that by using appropriate solvent conditions, placement of acyl groups can be controlled to achieve either random or M-selective substitution.
Alginate acetates synthesized in an M-selective fashion were used to study the ability of these derivatives to form Ca-crosslinked hydrogels. Detailed structure-property analyses were performed to identify acetylation reaction conditions and product properties that may be ideal for hydrogel formation. Furthermore,
alginate esters were synthesized via modification of carboxylate groups on the backbone. These derivatives dissolved in polar aprotic solvents without the need to add TBAF. A proof of concept study showed their utility in the solubility enhancement of the poorly water soluble flavonoid naringenin.
Advisors/Committee Members: Edgar, Kevin J. (committeechair), Turner, S. Richard (committee member), Frazier, Charles E. (committee member), Roman, Maren (committee member), Riffle, Judy S. (committee member).
Subjects/Keywords: Alginate; derivatization; regioselective; acetate; modification; DS; gelling
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APA (6th Edition):
Pawar, S. N. (2013). Chemical Modification of Alginates in Organic Media. (Doctoral Dissertation). Virginia Tech. Retrieved from http://hdl.handle.net/10919/52635
Chicago Manual of Style (16th Edition):
Pawar, Siddhesh Nitin. “Chemical Modification of Alginates in Organic Media.” 2013. Doctoral Dissertation, Virginia Tech. Accessed January 27, 2021.
http://hdl.handle.net/10919/52635.
MLA Handbook (7th Edition):
Pawar, Siddhesh Nitin. “Chemical Modification of Alginates in Organic Media.” 2013. Web. 27 Jan 2021.
Vancouver:
Pawar SN. Chemical Modification of Alginates in Organic Media. [Internet] [Doctoral dissertation]. Virginia Tech; 2013. [cited 2021 Jan 27].
Available from: http://hdl.handle.net/10919/52635.
Council of Science Editors:
Pawar SN. Chemical Modification of Alginates in Organic Media. [Doctoral Dissertation]. Virginia Tech; 2013. Available from: http://hdl.handle.net/10919/52635
University of New South Wales
22.
Lu, Wei.
Alginate and cucurbit[n]uril as combined drug delivery system for albendazole: Experiments and mathematical modelling.
Degree: Physical, 2013, University of New South Wales
URL: http://handle.unsw.edu.au/1959.4/53119
;
https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11805/SOURCE01?view=true
► A combined hydrogel-based drug delivery system consisting of alginate and cucurbit[n]uril (Q[n]) was prepared for the delivery of albendazole (ABZ), a sparingly soluble anthelmintic recently…
(more)
▼ A combined hydrogel-based drug delivery system consisting of
alginate and cucurbit[n]uril (Q[n]) was prepared for the delivery of albendazole (ABZ), a sparingly soluble anthelmintic recently found to have anti-cancer activity. Q[n] is a family of macrocyclic host molecules that has two roles in the delivery system described within this thesis. The smaller homologue, Q[5], served as an ionic cross-linker with two sodium ions bonded at both of its portals. The larger homologues, Q[7] and Q[8], encapsulated ABZ in their cavities. The encapsulation significantly increased the solubility of ABZ in water and achieved considerable drug loading content within the hydrogel, with a relatively high loading efficiency around 40%.Precursor solutions were prepared by dissolving sodium
alginate in saturated Q[5] solutions. Different amounts of the drug complexes were dissolved to give precursor solutions of different formulation. The hydrogel beads were best form by dripping the precursor solutions into a pH1 gelling solution. The beads exhibited the same pH responsive swelling behaviour as calcium
alginate hydrogel.Drug release studies were conducted in release media of low and high NaCl concentrations at three pH conditions of 6.3, 3 and 1. Percentage of the loaded drug released and drug release rates were found to be highly sensitive to the concentration of NaCl and pH but were also affected by other factors such as the type (size of the homologue used) and the amount of the drug complexes loaded into the gel. Complete release of the drug could be achieved only in the presence of ions, suggesting substantial electrostatic attraction between the drug complexes, the cross-linking Q[5] and the
alginate.Experimental drug release data were analysed by mathematical models, which suggested that drug release was primarily controlled by diffusion, with a swelling controlled component, where ions were present. The fitted effective diffusion coefficient of the drug complexes ranged between ~3×10-8 and 7×10-7 cm2/s, depending on the type and the amount of the drug complexes loaded and the nature of the release media.It is proposed, based on the experimental results and the mathematical modelling, that both of the drug complexes formed aggregates with Q[5] through intermolecular interactions, which is a process initiated by electrostatic attraction. However,
[email protected][8] had stronger interactions and formed larger aggregates than
[email protected][7], and hence had a more retarded release.Slow release of ABZ over a few days was achieved in physiological saline with
[email protected][8] and
[email protected][7] offered faster release maintaining higher concentrations.
Advisors/Committee Members: Day, Anthony, Physical, Environmental & Mathematical Sciences, UNSW Canberra, UNSW, Woodward, Cliff, Physical, Environmental & Mathematical Sciences, UNSW Canberra, UNSW.
Subjects/Keywords: Cucurbituril; Hygrogel; Drug delivery; Albendazole; Alginate
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APA ·
Chicago ·
MLA ·
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Manager
APA (6th Edition):
Lu, W. (2013). Alginate and cucurbit[n]uril as combined drug delivery system for albendazole: Experiments and mathematical modelling. (Masters Thesis). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/53119 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11805/SOURCE01?view=true
Chicago Manual of Style (16th Edition):
Lu, Wei. “Alginate and cucurbit[n]uril as combined drug delivery system for albendazole: Experiments and mathematical modelling.” 2013. Masters Thesis, University of New South Wales. Accessed January 27, 2021.
http://handle.unsw.edu.au/1959.4/53119 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11805/SOURCE01?view=true.
MLA Handbook (7th Edition):
Lu, Wei. “Alginate and cucurbit[n]uril as combined drug delivery system for albendazole: Experiments and mathematical modelling.” 2013. Web. 27 Jan 2021.
Vancouver:
Lu W. Alginate and cucurbit[n]uril as combined drug delivery system for albendazole: Experiments and mathematical modelling. [Internet] [Masters thesis]. University of New South Wales; 2013. [cited 2021 Jan 27].
Available from: http://handle.unsw.edu.au/1959.4/53119 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11805/SOURCE01?view=true.
Council of Science Editors:
Lu W. Alginate and cucurbit[n]uril as combined drug delivery system for albendazole: Experiments and mathematical modelling. [Masters Thesis]. University of New South Wales; 2013. Available from: http://handle.unsw.edu.au/1959.4/53119 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11805/SOURCE01?view=true
University of New South Wales
23.
Xin, Yongjia.
Effect of calcium and iron(III) on membrane fouling under conditions typical of submerged membrane bioreactor treatment of wastewaters.
Degree: Civil & Environmental Engineering, 2015, University of New South Wales
URL: http://handle.unsw.edu.au/1959.4/55075
;
https://unsworks.unsw.edu.au/fapi/datastream/unsworks:36468/SOURCE02?view=true
► While the use of submerged membrane bioreactor (MBR) technology has risen dramatically during last decade, the most significant challenge still remaining is the reduction in…
(more)
▼ While the use of submerged membrane bioreactor (MBR) technology has risen dramatically during last decade, the most significant challenge still remaining is the reduction in the severity of membrane fouling. In addition, this filtration technology performs poorly in removal of dissolved contaminants such as phosphorus with addition of adsorbing chemicals such as iron necessary to ensure satisfactory effluent quality. The addition of chemicals such as iron salts, which readily hydrolyse and precipitate as iron oxyhydroxides on addition to the wastewater stream, may however exacerbate the fouling problem, particularly in submerged MBRs where a sedimentation step is not used. In such situations, optimizing the dosage of iron salts is critical in ensuring the most cost effective performance of the MBR however, the relationship between iron dosage and membrane fouling is not completely understood. In particular, the presence of other wastewater constituents such as monovalent and divalent ions (such as sodium and calcium) may significantly influence the interaction of iron with soluble microbial products (SMP) present in the MBR supernatant. In this thesis, the model polysaccharide
alginate is used to investigate the interplay between SMP and iron and calcium under conditions typical of a submerged membrane bioreactor. The concentration of calcium present is shown to be a critical determinant of the severity of membrane fouling with low concentrations inducing
alginate gelation and resultant severe membrane fouling while higher calcium concentrations result in gel breakage and
alginate aggregation resulting in formation of porous cakes which facilitate rapid filtration. Our results also demonstrate that the presence of sodium may lead to a worsening of fouling as these ions block binding sites and limit the ability of calcium to induce aggregation. Comparison of the properties of the
alginate assemblages formed in the presence of iron indicate that the Fe-
alginate deposits induce even more severe fouling than Ca-
alginate gels with lower concentrations of iron than calcium required to induce gelation. Increasing the concentration of iron leads eventually to a reduction in fouling propensity, most likely as a result of the adsorption of
alginate to oxyhydroxide surfaces rather than
alginate bridging as was the case for calcium. Importantly, the presence of calcium in a system to which iron salts are dosed is shown to lead to a significant reduction in fouling propensity. Investigations with SMP from an actual wastewater plant reveal similar interplay with iron and calcium as observed in the
alginate system and highlight the possibility of fouling control through careful manipulation of iron and calcium concentrations in the supernatant.
Advisors/Committee Members: Waite, T. David, Civil & Environmental Engineering, Faculty of Engineering, UNSW.
Subjects/Keywords: Calcium; Membrane fouling; Alginate; Iron (III)
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APA (6th Edition):
Xin, Y. (2015). Effect of calcium and iron(III) on membrane fouling under conditions typical of submerged membrane bioreactor treatment of wastewaters. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/55075 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:36468/SOURCE02?view=true
Chicago Manual of Style (16th Edition):
Xin, Yongjia. “Effect of calcium and iron(III) on membrane fouling under conditions typical of submerged membrane bioreactor treatment of wastewaters.” 2015. Doctoral Dissertation, University of New South Wales. Accessed January 27, 2021.
http://handle.unsw.edu.au/1959.4/55075 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:36468/SOURCE02?view=true.
MLA Handbook (7th Edition):
Xin, Yongjia. “Effect of calcium and iron(III) on membrane fouling under conditions typical of submerged membrane bioreactor treatment of wastewaters.” 2015. Web. 27 Jan 2021.
Vancouver:
Xin Y. Effect of calcium and iron(III) on membrane fouling under conditions typical of submerged membrane bioreactor treatment of wastewaters. [Internet] [Doctoral dissertation]. University of New South Wales; 2015. [cited 2021 Jan 27].
Available from: http://handle.unsw.edu.au/1959.4/55075 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:36468/SOURCE02?view=true.
Council of Science Editors:
Xin Y. Effect of calcium and iron(III) on membrane fouling under conditions typical of submerged membrane bioreactor treatment of wastewaters. [Doctoral Dissertation]. University of New South Wales; 2015. Available from: http://handle.unsw.edu.au/1959.4/55075 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:36468/SOURCE02?view=true
University of Toledo Health Science Campus
24.
Shin, Junkyu.
Evaluation of Calcium Alginate Microparticles Prepared Using
a Novel Nebulized Aerosol Mediated Interfacial Crosslinking
Method.
Degree: MSP, Pharmaceutical Sciences (Industrial
Pharmacy), 2016, University of Toledo Health Science Campus
URL: http://rave.ohiolink.edu/etdc/view?acc_num=mco1469041620
► Alginate is a very popular biopolymer used in microencapsulation. Applications of alginate based microparticles can be found in pharmaceuticals, food, cosmetics, and tissue engineering industries.…
(more)
▼ Alginate is a very popular biopolymer used in
microencapsulation. Applications of
alginate based microparticles
can be found in pharmaceuticals, food, cosmetics, and tissue
engineering industries. In this work a novel nebulized aerosol
mediated interfacial crosslinking method was developed and
evaluated as a new potential particle formulation method. The
apparatus design for microparticle production, and the recovery
process was established. The evaluation studies such as particle
sizing, zeta potential, morphology observations by optical
microscopy, scanning electron microscopy, and atomic force
microscopy, thermal behavior by dynamic scanning calorimetry,
structural composition by Fourier transform infrared spectroscopy,
and encapsulation efficiency, were performed to characterize the
microparticle. A granular shape with embossed bubble surface
texture was observed in optical micrographs and SEM. The particle
size range was between 9.9 µm and 60.5 µm. The drug loading was
15.1 ± 3.1 µg of methylene blue per 1 mg of
microparticle.
Advisors/Committee Members: Nesamony, Jerry (Committee Chair).
Subjects/Keywords: Pharmaceuticals; calcium alginate, microparticle, nebulization,
ionotropic
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APA (6th Edition):
Shin, J. (2016). Evaluation of Calcium Alginate Microparticles Prepared Using
a Novel Nebulized Aerosol Mediated Interfacial Crosslinking
Method. (Masters Thesis). University of Toledo Health Science Campus. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=mco1469041620
Chicago Manual of Style (16th Edition):
Shin, Junkyu. “Evaluation of Calcium Alginate Microparticles Prepared Using
a Novel Nebulized Aerosol Mediated Interfacial Crosslinking
Method.” 2016. Masters Thesis, University of Toledo Health Science Campus. Accessed January 27, 2021.
http://rave.ohiolink.edu/etdc/view?acc_num=mco1469041620.
MLA Handbook (7th Edition):
Shin, Junkyu. “Evaluation of Calcium Alginate Microparticles Prepared Using
a Novel Nebulized Aerosol Mediated Interfacial Crosslinking
Method.” 2016. Web. 27 Jan 2021.
Vancouver:
Shin J. Evaluation of Calcium Alginate Microparticles Prepared Using
a Novel Nebulized Aerosol Mediated Interfacial Crosslinking
Method. [Internet] [Masters thesis]. University of Toledo Health Science Campus; 2016. [cited 2021 Jan 27].
Available from: http://rave.ohiolink.edu/etdc/view?acc_num=mco1469041620.
Council of Science Editors:
Shin J. Evaluation of Calcium Alginate Microparticles Prepared Using
a Novel Nebulized Aerosol Mediated Interfacial Crosslinking
Method. [Masters Thesis]. University of Toledo Health Science Campus; 2016. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=mco1469041620
25.
Chaubaroux, Christophe.
Revêtements à base de collagène pour la fonctionnalisation de biomatériaux : Collagen-based coatings for biomaterials functionalization.
Degree: Docteur es, Chimie physique, 2013, Université de Strasbourg
URL: http://www.theses.fr/2013STRAE004
► La fonctionnalisation des biomatériaux est une stratégie probante et prometteuse développée pour favoriser l’intégration de biomatériaux dans un organisme vivant. Le dépôt de films multicouches…
(more)
▼ La fonctionnalisation des biomatériaux est une stratégie probante et prometteuse développée pour favoriser l’intégration de biomatériaux dans un organisme vivant. Le dépôt de films multicouches de polyélectrolytes est une méthode de fonctionnalisation de surfaces particulièrement adaptée au recouvrement d’implants. Ces surfaces modifiées pourront ainsi interagir avec leur environnement biologique. Au cours de ce travail de thèse, nous avons développé un nouveau revêtement, à base de composés naturels, capable de recouvrir plusieurs types de surfaces. De plus, ces revêtements originaux peuvent être utilisés pour orienter certains phénomènes cellulaires. Dans la première partie de ce travail de thèse, nous avons mis au point un nouveau système de films multicouches à base de collagène et d’alginate. La réticulation chimique avec la génipine, un agent naturel d’origine végétale (Gardenia Jasminoide), stabilise ces constructions pour une utilisation en conditions physiologiques. Les études d’adhérence et de prolifération de cellules endothéliales humaines ont montré que ces revêtements à base de constituants naturels sont des supports adéquats en vue d’applications biomédicales. Nous avons ensuite déposé des films collagène/alginate sur des implants en titane précédemment recouvert d’un gel microporeux en poly(acide lactique). Nous avons pu montrer que les films collagène/alginate favorisent la prolifération de cellules épithéliales, ce qui permettrait une meilleure intégration des implants. Dans la deuxième partie de notre travail, nous avons développé une technique permettant d’obtenir des revêtements et des membranes à base de films multicouches collagène/alginate ayant des structures fibrillaires orientées. L’alignement fibrillaire s’obtient par simple étirement des substrats élastiques en poly(diméthyl siloxane) (PDMS) sur lesquels sont déposés les revêtements collagène/alginate. La déformation longitudinale du substrat induit un alignement préférentiel des fibrilles de collagène du revêtement. L’étude de l’influence du taux d’étirement sur l’alignement des fibres a montré qu’il était possible de moduler cet alignement. Enfin, nous avons observé que le comportement de différents types cellulaires (fibroblastes et astrocytes) est modifié par l’alignement fibrillaire. On note que les cellules s’alignent dans la même direction que les fibrilles de collagène. A l’évidence, l’organisation fibrillaire du revêtement conditionne la géométrie de l’étalement cellulaire. Les cellules s’allongent lorsque les fibrilles sont alignées. De plus, il apparaît que la direction des divisions cellulaires est guidée par la direction de l’alignement des fibrilles de collagène dans le revêtement étiré. Cela signifie que les cellules sont guidées par les fibrilles de collagène alignées.
Biomaterial functionalization is a promising strategy developed to favor material implantation in a living organism. The deposition of polyelectrolyte multilayer films is a useful functionalization technique to coat implants. These modified surfaces may…
Advisors/Committee Members: Hemmerlé, Joseph (thesis director), Haïkel, Youssef (thesis director).
Subjects/Keywords: Collagène; Alginate; Multicouches; Alignement; Fonctionnalisation; Collagen; Alginate; Multilayer; Alignment; Functionalization; 541.33; 571.4
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APA ·
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MLA ·
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CSE |
Export
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Manager
APA (6th Edition):
Chaubaroux, C. (2013). Revêtements à base de collagène pour la fonctionnalisation de biomatériaux : Collagen-based coatings for biomaterials functionalization. (Doctoral Dissertation). Université de Strasbourg. Retrieved from http://www.theses.fr/2013STRAE004
Chicago Manual of Style (16th Edition):
Chaubaroux, Christophe. “Revêtements à base de collagène pour la fonctionnalisation de biomatériaux : Collagen-based coatings for biomaterials functionalization.” 2013. Doctoral Dissertation, Université de Strasbourg. Accessed January 27, 2021.
http://www.theses.fr/2013STRAE004.
MLA Handbook (7th Edition):
Chaubaroux, Christophe. “Revêtements à base de collagène pour la fonctionnalisation de biomatériaux : Collagen-based coatings for biomaterials functionalization.” 2013. Web. 27 Jan 2021.
Vancouver:
Chaubaroux C. Revêtements à base de collagène pour la fonctionnalisation de biomatériaux : Collagen-based coatings for biomaterials functionalization. [Internet] [Doctoral dissertation]. Université de Strasbourg; 2013. [cited 2021 Jan 27].
Available from: http://www.theses.fr/2013STRAE004.
Council of Science Editors:
Chaubaroux C. Revêtements à base de collagène pour la fonctionnalisation de biomatériaux : Collagen-based coatings for biomaterials functionalization. [Doctoral Dissertation]. Université de Strasbourg; 2013. Available from: http://www.theses.fr/2013STRAE004
University of Saskatchewan
26.
Zhai, Peng.
Alginate Microspheres for Protein Delivery in Tissue Engineering.
Degree: 2012, University of Saskatchewan
URL: http://hdl.handle.net/10388/ETD-2012-05-461
► Three-dimensional polymeric scaffolds have been widely used in tissue engineering for protein delivery. Scaffolds fabricated with different biomaterials and structures display various functions in protein…
(more)
▼ Three-dimensional polymeric scaffolds have been widely used in tissue engineering for protein delivery. Scaffolds fabricated with different biomaterials and structures display various functions in protein delivery. A microsphere based delivery system is one sophisticated method. In this research, the potentials of
alginate microspheres as protein carriers were tested.
Alginate microspheres were prepared by a water-in-oil emulsion external gelation technique and loaded with bovine serum albumin (BSA) or DyLight 800 dye-labeled rabbit immunoglobulin G (IgG). Chitosan coated protein-loaded
alginate microspheres were also prepared. The effects of process parameters on microsphere size, size distribution, encapsulation efficiency, and in vitro protein release profiles were investigated. Scanning electron microscopic photos showed that high dispersing force and high calcium chloride concentration produce small and uniform
alginate microspheres with spherical shape and smooth surface. The release profiles indicated that BSA release from large and heterogeneous
alginate microspheres was rapid and had a large initial burst release, and IgG release from small and homogeneous microspheres was slower and had lower initial burst release. Chitosan coating caused slower protein release compared to uncoated
alginate microspheres in all cases. Protein-loaded microspheres were incorporated into
alginate cylindrical scaffolds by long-term moulding in tubing for BSA or by fast gelation extruded from tubing for IgG. The scaffolds contained dried BSA loaded microspheres prepared using either protein incorporation or incubation methods, or with wet IgG-loaded microspheres with different concentrations. The release studies of BSA indicated that dried microspheres provided fast release possibly. The IgG release from scaffolds illustrated that chitosan coated IgG microspheres had more prolonged release profiles, and fast gelation of scaffolds could potentially eliminate protein loss during long-term gelation. All release profiles of scaffolds demonstrated that the initial burst effect was diminished and the release was extended by using a delivery system in which microspheres were incorporated into larger scaffolds. These prepared
alginate microspheres and microsphere-incorporated scaffolds have been proven to have abilities of carrying and releasing proteins. Their applications toward delivery of functional proteins at the target site in patients for therapeutic purposes should be considered.
Advisors/Committee Members: Chen, Daniel X. B., Schreyer, David J..
Subjects/Keywords: Alginate microspheres; Uniform size; Chitosan coating; Protein release; Alginate scaffolds; Tissue engineering
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APA ·
Chicago ·
MLA ·
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CSE |
Export
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Manager
APA (6th Edition):
Zhai, P. (2012). Alginate Microspheres for Protein Delivery in Tissue Engineering. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2012-05-461
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Zhai, Peng. “Alginate Microspheres for Protein Delivery in Tissue Engineering.” 2012. Thesis, University of Saskatchewan. Accessed January 27, 2021.
http://hdl.handle.net/10388/ETD-2012-05-461.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Zhai, Peng. “Alginate Microspheres for Protein Delivery in Tissue Engineering.” 2012. Web. 27 Jan 2021.
Vancouver:
Zhai P. Alginate Microspheres for Protein Delivery in Tissue Engineering. [Internet] [Thesis]. University of Saskatchewan; 2012. [cited 2021 Jan 27].
Available from: http://hdl.handle.net/10388/ETD-2012-05-461.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Zhai P. Alginate Microspheres for Protein Delivery in Tissue Engineering. [Thesis]. University of Saskatchewan; 2012. Available from: http://hdl.handle.net/10388/ETD-2012-05-461
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Université de Grenoble
27.
Dalle, Prisca.
Système intégré pour l'encapsulation monocouche de cellules : Integrated system for simple layer cell encapsulation.
Degree: Docteur es, Biotechnologie, instrumentation, signal et imagerie pour la biologie, la médecine, et l’environnement, 2012, Université de Grenoble
URL: http://www.theses.fr/2012GRENS036
► L'implantation d'îlots de Langerhans microencapsulés est une thérapie prometteuse pour le diabète de type 1. Le concept est de rétablir la fonction de sécrétion d'insuline…
(more)
▼ L'implantation d'îlots de Langerhans microencapsulés est une thérapie prometteuse pour le diabète de type 1. Le concept est de rétablir la fonction de sécrétion d'insuline tout en évitant le rejet de greffe. Les premiers essais cliniques ont reporté des résultats encourageants mais encore peu reproductibles. En effet, les techniques actuelles de production de microcapsules sont manuelles et au sein d'un même lot les capsules ne sont pas homogènes. L'utilisation de la microfluidique offre la possibilité de remédier à ce problème. Ce projet présente les différentes étapes d'optimisation d'un système microfluidique complexe qui automatise le procédé d'encapsulation monocouche de cellules dans des capsules polymériques d'alginate. Ce système comporte trois fonctions principales en série : la formation de gouttes monodisperses d'alginate en flux d'huile par un module MFFD (Micro Flow Focusing Device), le transfert de ces gouttes de la phase huileuse vers une phase aqueuse gélifiante de calcium et enfin un second transfert vers un flux à concentration physiologique. Des capsules monodisperses et sphériques ont été obtenues en sortie de ce système et des premiers tests d'encapsulation de cellules ont été réalisés. Les capsules produites par le système automatisé ont conduit à une première implantation chez le rongeur. Ce système est une première étape clé vers un dispositif instrumenté qui permettrait aux cliniciens d'encapsuler des îlots rapidement et de façon reproductible directement après leur isolement, puis de les implanter chez les patients diabétiques. Mots clés : encapsulation, îlots de Langerhans, alginate, gélification, microfluidique, automatisation, MFFD, transfert de phase.
Epileptic seizures arise from pathological synchronization of neuronal ensemble.Seizures originating from primary motor cortex are often pharmacoresistant, and many times unsuitable for respective surgery because of location of epileptic focus in eloquent area. Basal ganglia play important role in seizure propagation. Micro electrode recordings performed during previous studies indicated that input structures of basal ganglia such as GPe, Putamen and Subthalamic nucleus (STN) are strongly modified during seizures. For example the mean firing rate of neurons of the STN and Putamen increased and the percentage of oscillatory neurons synchronized with the ictal EEG was higher during seizures as compared to interictal periods. Pilot studies in humans have shown the possible beneficial effect of chronic DBS applied to STN in treatment of pharmacoresistant motor seizures. Our study was aimed at studying the therapeutic effect of electrical stimulation of input structures of basal ganglia . We first developed a stable, predictable primate model of focal motor epilepsy by intracortical injection of penicillin and we documented it's pharmacoresistence. We then stereotactically implanted DBS electrodes in the STN and Putamen. The stimulator was embedded at the back of the animals. Subthreshold electrical stimulations at 130 Hz were applied to STN.…
Advisors/Committee Members: Benhamou, Pierre-Yves (thesis director), Rivera, Florence (thesis director).
Subjects/Keywords: Microfluidique; Encapsulation; Gélification; Alginate; Cellules; In vivo; Microfluidic; Encaspsulation; Gelation; Alginate; Cell; In vivo
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APA (6th Edition):
Dalle, P. (2012). Système intégré pour l'encapsulation monocouche de cellules : Integrated system for simple layer cell encapsulation. (Doctoral Dissertation). Université de Grenoble. Retrieved from http://www.theses.fr/2012GRENS036
Chicago Manual of Style (16th Edition):
Dalle, Prisca. “Système intégré pour l'encapsulation monocouche de cellules : Integrated system for simple layer cell encapsulation.” 2012. Doctoral Dissertation, Université de Grenoble. Accessed January 27, 2021.
http://www.theses.fr/2012GRENS036.
MLA Handbook (7th Edition):
Dalle, Prisca. “Système intégré pour l'encapsulation monocouche de cellules : Integrated system for simple layer cell encapsulation.” 2012. Web. 27 Jan 2021.
Vancouver:
Dalle P. Système intégré pour l'encapsulation monocouche de cellules : Integrated system for simple layer cell encapsulation. [Internet] [Doctoral dissertation]. Université de Grenoble; 2012. [cited 2021 Jan 27].
Available from: http://www.theses.fr/2012GRENS036.
Council of Science Editors:
Dalle P. Système intégré pour l'encapsulation monocouche de cellules : Integrated system for simple layer cell encapsulation. [Doctoral Dissertation]. Université de Grenoble; 2012. Available from: http://www.theses.fr/2012GRENS036
28.
Lembré, Pierre.
Contribution à l’étude de l’organisation et des propriétés mécaniques d’exopolymères de matrice de biofilms modèles : Contribution to the study of the organization and the mechanical properties of biofilms exopolymer matrix models.
Degree: Docteur es, Sciences de la vie et de la santé - Cergy, 2012, Cergy-Pontoise
URL: http://www.theses.fr/2012CERG0612
► Les biofilms sont des édifices macromoléculaires qui résultent de l'adhérence de microorganismes à une surface. Ils sont constitués de cellules enchâssées dans un réseau d'exopolymères…
(more)
▼ Les biofilms sont des édifices macromoléculaires qui résultent de l'adhérence de microorganismes à une surface. Ils sont constitués de cellules enchâssées dans un réseau d'exopolymères d'origine biologique qui forment une matrice extracellulaire. Les biofilms posent des problèmes technologiques et sanitaires dans de nombreux domaines, aussi bien agroalimentaire, médical, ou industriel. Comprendre les mécanismes de formation de ces structures est donc un enjeu majeur. Malgré une grande diversité de la structure des biofilms, de grands principes semblent en régir la composition. Ainsi, la présence de polysaccharides comme l'alginate et la cellulose joue un rôle majeur dans leur formation et dans la définition de leurs propriétés mécaniques. Si la présence de polymères protéiques comme les fibres amyloïdes semble avoir un caractère universel au sein des biofilms, leur rôle dans la formation de la matrice et dans ses propriétés mécaniques restait à définir. Lors de cette étude, nous avons caractérisé la structure et la composition de trois biofilms monobactériens issus de trois espèces différentes P. aeruginosa NK 125502, S. enterica CIP 58.58 et S. epidermidis CIP 53.124. Nous avons décrit la formation de fibres amyloïdes par différents peptides issus de protéines bactériennes impliquées dans la formation de biofilm et montré par différentes techniques qu'ils sont capables de former des fibres amyloïdes. Nous avons notamment identifié un peptide amyloïde, suggérant la présence de ce type de fibre au sein des biofilms de Staphylococcus, mais aussi plus généralement dans les biofilms des espèces exprimant une protéine de type Bap (Biofilm associated protein). Enfin, nous avons analysé les propriétés mécaniques de différentes matrices synthétiques à base d'alginate et de méthyl-cellulose, en présence et en absence de protéines et de peptides amyloïdes afin de mieux comprendre l'apport qu'a ce type de fibre sur les propriétés de ces structures. Ainsi, les fibres amyloïdes modifient les propriétés mécaniques des gels synthétiques, permettant d'augmenter la déformation sous contrainte. En conclusion, ce travail apporte de nouveaux éléments pour la compréhension du rôle des fibres amyloïdes dans le renforcement de la matrice du biofilm. La capacité à former des fibres amyloïdes par un peptide issu de la protéine Bap de S. epidermidis suggère que cette propriété est plus largement présente au sein de cette famille de protéines. Les travaux menés au cours de cette thèse, et l'ensemble des techniques utilisées, avec notamment la mise au point de l'observation de la biréfringence du rouge Congo par microscopie confocale permettront de développer les études sur cette famille de protéines amyloïdes ainsi que sur les matrices complexes de type biofilm
Biofilms are macromolecular structures which result from the adhesion of microorganisms to a surface. They consist of cells embedded in a network of exopolymers of biological origin which form an extracellular matrix. Biofilms pose technological and health problems in many industrial…
Advisors/Committee Members: Di Martino, Patrick (thesis director).
Subjects/Keywords: Biofilm; Matrice; Fibre amyloïde; Alginate; Cellulose; Viscoélasticité; Biofilm; Matrix; Amyloid fibers; Alginate; Cellulose; Viscoelasticity
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APA (6th Edition):
Lembré, P. (2012). Contribution à l’étude de l’organisation et des propriétés mécaniques d’exopolymères de matrice de biofilms modèles : Contribution to the study of the organization and the mechanical properties of biofilms exopolymer matrix models. (Doctoral Dissertation). Cergy-Pontoise. Retrieved from http://www.theses.fr/2012CERG0612
Chicago Manual of Style (16th Edition):
Lembré, Pierre. “Contribution à l’étude de l’organisation et des propriétés mécaniques d’exopolymères de matrice de biofilms modèles : Contribution to the study of the organization and the mechanical properties of biofilms exopolymer matrix models.” 2012. Doctoral Dissertation, Cergy-Pontoise. Accessed January 27, 2021.
http://www.theses.fr/2012CERG0612.
MLA Handbook (7th Edition):
Lembré, Pierre. “Contribution à l’étude de l’organisation et des propriétés mécaniques d’exopolymères de matrice de biofilms modèles : Contribution to the study of the organization and the mechanical properties of biofilms exopolymer matrix models.” 2012. Web. 27 Jan 2021.
Vancouver:
Lembré P. Contribution à l’étude de l’organisation et des propriétés mécaniques d’exopolymères de matrice de biofilms modèles : Contribution to the study of the organization and the mechanical properties of biofilms exopolymer matrix models. [Internet] [Doctoral dissertation]. Cergy-Pontoise; 2012. [cited 2021 Jan 27].
Available from: http://www.theses.fr/2012CERG0612.
Council of Science Editors:
Lembré P. Contribution à l’étude de l’organisation et des propriétés mécaniques d’exopolymères de matrice de biofilms modèles : Contribution to the study of the organization and the mechanical properties of biofilms exopolymer matrix models. [Doctoral Dissertation]. Cergy-Pontoise; 2012. Available from: http://www.theses.fr/2012CERG0612
INP Toulouse
29.
Monteiro Centeno da Costa, Filipe.
Procédé d’immobilisation de levures pour applications oenologiques. Etudes des paramètres du procédé. Validations experimentales : Yeast immobilisation process for oenological applications. Process parameters. Experimental validations.
Degree: Docteur es, Génie des procédés et environnement, 2011, INP Toulouse
URL: http://www.theses.fr/2011INPT0061
► L'étude et le développement des procédés de fabrication de levures immobilisées en vue de la réalisation de fermentations de vins a débuté au milieu des…
(more)
▼ L'étude et le développement des procédés de fabrication de levures immobilisées en vue de la réalisation de fermentations de vins a débuté au milieu des années 80. Malgré les bénéfices potentiels que cette technologie pouvait apporter pour le secteur œnologique, peu de procédés d'immobilisation ont réussi à dépasser l'échelle laboratoire ou pilote et ceux qui sont arrivés à l'échelle industrielle n'ont pas eu le succès désiré pour des questions d'ordre technique ou économique. Le premier objectif de ce travail concerne la mise au point du procédé industriel en insistant sur les aspects les plus sensibles, et qui comme tels ont exigé des études complémentaires. Le deuxième objectif de ce travail vise à caractériser du point de vue cinétique et lorsque possible sensoriel, les fermentations avec les levures immobilisées pour la production de vins effervescents et pour la désacidification biologique de moûts. Le troisième et dernier objectif de ce travail consiste à évaluer l'utilisation de levures immobilisées pour la réalisation de la fermentation alcoolique en continu de moût. Pour cela on a fait appel à des fermenteurs continus à lit fixe et à lit fluidisé.
The study and development of yeast immobilization processes for wine fermentations started in the mid 80’s. Even though this technology could be of great benefit for the oenological sector very few process left the laboratory or pilot scale and those which arrived to industrial scale didn’t have the ambitioned success due to technical or economical constraints. The first goal of this work was to develop an industrial process for yeast immobilisation with emphasis on the most sensitive aspects which required further studies. The second objective of this work was to characterise the fermentation kinetics of immobilised yeasts cells during the production sparkling wines and during the deacidification of grape must. Whenever possible the wines produced were also characterised from a sensorial point of view. The third and last goal was to evaluate the use of immobilised yeast cells for continuous fermentation of grape must. For that we have used continuous fixed bed and fluidized bed fermenters.
Advisors/Committee Members: Strehaiano, Pierre (thesis director), Alliet, Marion (thesis director).
Subjects/Keywords: Immobilisation; Levures immobilisées fermentation; Alginate; Vin; Immobilization; Immobilized yeast fermentation; Alginate; Wine
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APA (6th Edition):
Monteiro Centeno da Costa, F. (2011). Procédé d’immobilisation de levures pour applications oenologiques. Etudes des paramètres du procédé. Validations experimentales : Yeast immobilisation process for oenological applications. Process parameters. Experimental validations. (Doctoral Dissertation). INP Toulouse. Retrieved from http://www.theses.fr/2011INPT0061
Chicago Manual of Style (16th Edition):
Monteiro Centeno da Costa, Filipe. “Procédé d’immobilisation de levures pour applications oenologiques. Etudes des paramètres du procédé. Validations experimentales : Yeast immobilisation process for oenological applications. Process parameters. Experimental validations.” 2011. Doctoral Dissertation, INP Toulouse. Accessed January 27, 2021.
http://www.theses.fr/2011INPT0061.
MLA Handbook (7th Edition):
Monteiro Centeno da Costa, Filipe. “Procédé d’immobilisation de levures pour applications oenologiques. Etudes des paramètres du procédé. Validations experimentales : Yeast immobilisation process for oenological applications. Process parameters. Experimental validations.” 2011. Web. 27 Jan 2021.
Vancouver:
Monteiro Centeno da Costa F. Procédé d’immobilisation de levures pour applications oenologiques. Etudes des paramètres du procédé. Validations experimentales : Yeast immobilisation process for oenological applications. Process parameters. Experimental validations. [Internet] [Doctoral dissertation]. INP Toulouse; 2011. [cited 2021 Jan 27].
Available from: http://www.theses.fr/2011INPT0061.
Council of Science Editors:
Monteiro Centeno da Costa F. Procédé d’immobilisation de levures pour applications oenologiques. Etudes des paramètres du procédé. Validations experimentales : Yeast immobilisation process for oenological applications. Process parameters. Experimental validations. [Doctoral Dissertation]. INP Toulouse; 2011. Available from: http://www.theses.fr/2011INPT0061
30.
Ben Amara, Chedia.
Étude des interactions polysaccharides – biomolécules antimicrobiennes de nature protéique : application à l’élaboration de microcapsules et de films actifs pour la conservation des aliments : Study of the interactions polysaccharides - antimicrobial biomolecules of protein nature : application to the development of microcapsules and active films for food preservation.
Degree: Docteur es, Sciences des aliments, 2017, Lyon
URL: http://www.theses.fr/2017LYSE1247
► Ce travail porte sur le développement de systèmes d’encapsulation à base de polysaccharides et de molécules antimicrobiennes de nature protéique comme le lysozyme du blanc…
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▼ Ce travail porte sur le développement de systèmes d’encapsulation à base de polysaccharides et de molécules antimicrobiennes de nature protéique comme le lysozyme du blanc d’œuf et la nisine produite par la bactérie Lactococcus lactis. La coacervation complexe de l’alginate ou la pectine (deux polysaccharides anioniques) avec le lysozyme ou la nisine, chargés positivement sur une large gamme de pH, serait une solution pour protéger ces molécules et assurer une libération contrôlée lors de la conservation d’un aliment. Les polysaccharides utilisés ont été choisis en fonction de leur sensibilité aux enzymes produites par les bactéries cibles. L’ensemble des travaux menés à différentes échelles a mis en évidence (i) le rôle de certains facteurs physico-chimiques (ratio, pH, force ionique,…) sur les interactions mises en jeu entre le lysozyme ou la nisine et l’alginate ou la pectine, (ii) l’influence de ces facteurs sur les propriétés des complexes formés et (iii) le rôle des polysaccharides sélectionnés dans la stabilisation de la structure du lysozyme ou de la nisine lors du séchage par atomisation. Enfin, la structure et l’activité antimicrobienne des films obtenus par voie solvant (casting) et des microcapsules obtenues par atomisation sont étudiées en relation avec les propriétés des complexes formés. Ce travail a permis une meilleure compréhension des mécanismes impliqués dans la formation des complexes peptides/polysaccharides ou protéines/polysaccharides, leur résistance au séchage par atomisation ainsi que leur capacité à protéger et à libérer des molécules actives
This work deals with the development of encapsulation systems based on polysaccharides and antimicrobial molecules of protein nature such as hen egg-white lysozyme and nisin produced by Lactococcus lactis. Complex coacervation of alginate or pectin (two anionic polysaccharides) with lysozyme or nisin, positively charged over a wide range of pH, would be a solution to protect these molecules and ensure their controlled release during food preservation. The used polysaccharides were chosen according to their sensitivity to the enzymes produced by the targeted bacteria. The whole of this work performed at various levels showed (i) the impact of some physicochemical factors (ratio, pH, ionic strength,…) on the interactions between lysozyme or nisin and alginate or pectin, (ii) the influence of these factors on the properties of the formed complexes, and (iii) the role of the selected polysaccharides in stabilizing the structure of lysozyme or nisin during spray-drying. Finally, the structure and the antimicrobial activity of films produced by casting and microcapsules obtained by spray-drying were studied in relation to the properties of the formed complexes. This work makes it possible to contribute to a better understanding of the various mechanisms implied in the formation of peptide/polysaccharide or protein/polysaccharide complexes, their resistance to spray-drying and their ability to protect and release active molecules
Advisors/Committee Members: Degraeve, Pascal (thesis director), Gharsallaoui, Adem (thesis director).
Subjects/Keywords: Coacervation; Alginate; Pectine; Lysozyme; Nisine; Microencapsulation; Interactions; Coacervation; Alginate; Pectin; Lysozyme; Nisin; Microencapsulation; Interactions; 572
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APA ·
Chicago ·
MLA ·
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CSE |
Export
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APA (6th Edition):
Ben Amara, C. (2017). Étude des interactions polysaccharides – biomolécules antimicrobiennes de nature protéique : application à l’élaboration de microcapsules et de films actifs pour la conservation des aliments : Study of the interactions polysaccharides - antimicrobial biomolecules of protein nature : application to the development of microcapsules and active films for food preservation. (Doctoral Dissertation). Lyon. Retrieved from http://www.theses.fr/2017LYSE1247
Chicago Manual of Style (16th Edition):
Ben Amara, Chedia. “Étude des interactions polysaccharides – biomolécules antimicrobiennes de nature protéique : application à l’élaboration de microcapsules et de films actifs pour la conservation des aliments : Study of the interactions polysaccharides - antimicrobial biomolecules of protein nature : application to the development of microcapsules and active films for food preservation.” 2017. Doctoral Dissertation, Lyon. Accessed January 27, 2021.
http://www.theses.fr/2017LYSE1247.
MLA Handbook (7th Edition):
Ben Amara, Chedia. “Étude des interactions polysaccharides – biomolécules antimicrobiennes de nature protéique : application à l’élaboration de microcapsules et de films actifs pour la conservation des aliments : Study of the interactions polysaccharides - antimicrobial biomolecules of protein nature : application to the development of microcapsules and active films for food preservation.” 2017. Web. 27 Jan 2021.
Vancouver:
Ben Amara C. Étude des interactions polysaccharides – biomolécules antimicrobiennes de nature protéique : application à l’élaboration de microcapsules et de films actifs pour la conservation des aliments : Study of the interactions polysaccharides - antimicrobial biomolecules of protein nature : application to the development of microcapsules and active films for food preservation. [Internet] [Doctoral dissertation]. Lyon; 2017. [cited 2021 Jan 27].
Available from: http://www.theses.fr/2017LYSE1247.
Council of Science Editors:
Ben Amara C. Étude des interactions polysaccharides – biomolécules antimicrobiennes de nature protéique : application à l’élaboration de microcapsules et de films actifs pour la conservation des aliments : Study of the interactions polysaccharides - antimicrobial biomolecules of protein nature : application to the development of microcapsules and active films for food preservation. [Doctoral Dissertation]. Lyon; 2017. Available from: http://www.theses.fr/2017LYSE1247
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Open Access Theses and Dissertations