subject:(Alginate)

Victoria University of Wellington

1. Abbott, Lynaire Jane. Polysaccharide Content and Growth Rate of Lessonia Variegata J. Agardh: Investigating its Potential as a Commercial Species.

Degree: 2011, Victoria University of Wellington

URL: http://hdl.handle.net/10063/4527

► The endemic brown alga Lessonia variegata has recently been shown to be four separate lineages. To determine differences between the four morphologically similar lineages, the… (more)

Subjects/Keywords: Algae; Fucoidan; Alginate

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APA (6^th Edition):

Abbott, L. J. (2011). Polysaccharide Content and Growth Rate of Lessonia Variegata J. Agardh: Investigating its Potential as a Commercial Species. (Masters Thesis). Victoria University of Wellington. Retrieved from http://hdl.handle.net/10063/4527

Chicago Manual of Style (16^th Edition):

Abbott, Lynaire Jane. “Polysaccharide Content and Growth Rate of Lessonia Variegata J. Agardh: Investigating its Potential as a Commercial Species.” 2011. Masters Thesis, Victoria University of Wellington. Accessed August 22, 2019. http://hdl.handle.net/10063/4527.

MLA Handbook (7^th Edition):

Abbott, Lynaire Jane. “Polysaccharide Content and Growth Rate of Lessonia Variegata J. Agardh: Investigating its Potential as a Commercial Species.” 2011. Web. 22 Aug 2019.

Vancouver:

Abbott LJ. Polysaccharide Content and Growth Rate of Lessonia Variegata J. Agardh: Investigating its Potential as a Commercial Species. [Internet] [Masters thesis]. Victoria University of Wellington; 2011. [cited 2019 Aug 22]. Available from: http://hdl.handle.net/10063/4527.

Council of Science Editors:

Abbott LJ. Polysaccharide Content and Growth Rate of Lessonia Variegata J. Agardh: Investigating its Potential as a Commercial Species. [Masters Thesis]. Victoria University of Wellington; 2011. Available from: http://hdl.handle.net/10063/4527

Victoria University of Wellington

2. Ghosh, Sayani. Characterisation of the Genes Encoding Mannuronan-C5-Epimerase in the Brown Alga Lessonia Variegata.

Degree: 2012, Victoria University of Wellington

URL: http://hdl.handle.net/10063/2654

► Alginate is known to be a commercially valuable polysaccharide, of great importance in industries such as food, cosmetics, medicine and pharmaceuticals. It is obtained commercially… (more)

Subjects/Keywords: Alginate; Gene duplication; Phaeophyceae

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APA (6^th Edition):

Ghosh, S. (2012). Characterisation of the Genes Encoding Mannuronan-C5-Epimerase in the Brown Alga Lessonia Variegata. (Masters Thesis). Victoria University of Wellington. Retrieved from http://hdl.handle.net/10063/2654

Chicago Manual of Style (16^th Edition):

Ghosh, Sayani. “Characterisation of the Genes Encoding Mannuronan-C5-Epimerase in the Brown Alga Lessonia Variegata.” 2012. Masters Thesis, Victoria University of Wellington. Accessed August 22, 2019. http://hdl.handle.net/10063/2654.

MLA Handbook (7^th Edition):

Ghosh, Sayani. “Characterisation of the Genes Encoding Mannuronan-C5-Epimerase in the Brown Alga Lessonia Variegata.” 2012. Web. 22 Aug 2019.

Vancouver:

Ghosh S. Characterisation of the Genes Encoding Mannuronan-C5-Epimerase in the Brown Alga Lessonia Variegata. [Internet] [Masters thesis]. Victoria University of Wellington; 2012. [cited 2019 Aug 22]. Available from: http://hdl.handle.net/10063/2654.

Council of Science Editors:

Ghosh S. Characterisation of the Genes Encoding Mannuronan-C5-Epimerase in the Brown Alga Lessonia Variegata. [Masters Thesis]. Victoria University of Wellington; 2012. Available from: http://hdl.handle.net/10063/2654

Université de Montréal

3. Fournier St-Laurent, Samuel. Encapsulation de Dehalococcoides: avantage pour la déhalogénation des solvants chlorés en sites contaminés .

Degree: 2009, Université de Montréal

URL: http://hdl.handle.net/1866/3145

► Le tétrachloroéthène (PCE) et les éthènes chlorés qui lui sont apparentés ont été abondamment utilisés pour plusieurs applications en industrie dès le début du 20e… (more)

▼ Le tétrachloroéthène (PCE) et les éthènes chlorés qui lui sont apparentés ont été abondamment utilisés pour plusieurs applications en industrie dès le début du 20e siècle. Ils sont cependant comptés parmi les polluants les plus communs des sols et de l’eau et beaucoup d’efforts sont déployés afin de les éliminer. Nous croyons que la conversion des éthènes chlorés en éthènes par des microorganismes est une solution prometteuse. Le premier aspect du projet visait donc à établir les conditions pour lesquelles un consortium enrichi en Dehalococcoides ethenogenes permettrait la conversion complète de PCE en éthène. Les expériences réalisées nous ont permis de souligner le rôle de l’acide lactique ajouté aux cultures comme source de carbone et source indirecte d’électrons pour la déhalorespiration. Nous avons également pu établir l’effet de la concentration initiale de biomasse dans les cultures sur le profil de déhalogénation du PCE. Le deuxième aspect du projet visait à développer un protocole d’encapsulation du consortium dans une matrice polymérique afin de profiter des nombreux avantages potentiels de l’encapsulation. Nous avons testé trois montages d’encapsulation différents : atomisation avec jet d’air, atomisation avec vibrations ultrasoniques et « drop-wise ». Le dernier montage prévoyait l’encapsulation des cultures dans des billes d’alginate enrobées de chitosane gélifié par du lignosulfonate. C’est le seul montage qui nous a permis d’encapsuler le consortium de façon efficace sans effet significatifs négatifs sur son activité de déchlorination. Aussi, la comparaison des profils de déhalogénation du PCE de cellules encapsulées et cellules libres a montré une plus faible accumulation de TCE, 1,2-DCE et VC dans les échantillons de cellules encapsulée et, par conséquent, une conversion plus rapide et plus complète du PCE en éthène. Finalement, nous avons observé une tendance favorable à l’idée que les microorganismes encapsulés bénéficient d’un effet de protection contre de faibles concentrations d’oxygène. Advisors/Committee Members: Guiot, Serge (advisor).

Subjects/Keywords: Encapsulation; Biorémédiation; Bioremediation; Alginate; PCE

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APA (6^th Edition):

Fournier St-Laurent, S. (2009). Encapsulation de Dehalococcoides: avantage pour la déhalogénation des solvants chlorés en sites contaminés . (Thesis). Université de Montréal. Retrieved from http://hdl.handle.net/1866/3145

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fournier St-Laurent, Samuel. “Encapsulation de Dehalococcoides: avantage pour la déhalogénation des solvants chlorés en sites contaminés .” 2009. Thesis, Université de Montréal. Accessed August 22, 2019. http://hdl.handle.net/1866/3145.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fournier St-Laurent, Samuel. “Encapsulation de Dehalococcoides: avantage pour la déhalogénation des solvants chlorés en sites contaminés .” 2009. Web. 22 Aug 2019.

Vancouver:

Fournier St-Laurent S. Encapsulation de Dehalococcoides: avantage pour la déhalogénation des solvants chlorés en sites contaminés . [Internet] [Thesis]. Université de Montréal; 2009. [cited 2019 Aug 22]. Available from: http://hdl.handle.net/1866/3145.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fournier St-Laurent S. Encapsulation de Dehalococcoides: avantage pour la déhalogénation des solvants chlorés en sites contaminés . [Thesis]. Université de Montréal; 2009. Available from: http://hdl.handle.net/1866/3145

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Otago

4. Germoni, Leonardo Alexandro Piero. The effect of alginate lyase on the antibiotic sensitivity of mucoid Pseudomonas aeruginosa biofilms .

Degree: 2014, University of Otago

URL: http://hdl.handle.net/10523/4784

► Bacteria are single celled organisms that can live together in communities called biofilms. These biofilms consist of a structural matrix consisting of polysaccharide gel secreted… (more)

▼ Bacteria are single celled organisms that can live together in communities called biofilms. These biofilms consist of a structural matrix consisting of polysaccharide gel secreted by the bacteria, with the bacteria living within the confines of the gel. Biofilms can arise on all sorts of surfaces, including those of medical catheters and equipment as well as the tissues of the body itself. Biofilms act as reservoirs of infection allowing bacteria to set up chronic infections that resist both the host immune system and antibiotics. People with compromised defences, such as cystic fibrosis (CF) sufferers, are particularly vulnerable to infection, especially by biofilm forming bacteria. The lungs of CF patients have thickened, dehydrated lung mucus that prevents the lungs from clearing inhaled foreign particles, including bacteria. P. aeruginosa is an opportunistic pathogen of humans and one of the most common organisms that infect the lungs of individuals with CF. During chronic infection P. aeruginosa can overproduce alginate, a capsular, highly viscous polysaccharide. This polysaccharide forms a thick capsule around the bacteria which retains water and also prevents complement-mediated killing and phagocytosis by neutrophils. Alginate has been linked to antibiotic resistance by some researchers while others have shown alginate plays almost no role in antibiotic resistance. Alginate can be broken down by the enzyme alginate lyase which eliminates the glycosidic bond between two sugar residues in the polysaccharide chain. The aim of this study was to compare and characterise the effects of two different alginate lyase enzymes on the structure of P. aeruginosa biofilms and the sensitivity of biofilm bacteria to antibiotics. The alginate lyases used in this study were a commercially available lyase from Sphingobacterium multivorans (SBAL) and the ALYI-III alginate lyase from Sphingomonas sp. A1 (ALY) produced via recombinant gene expression in E. coli. The recombinant protein included a 6x Histidine tag on the N-terminal sequence and was purified using immobilised metal affinity chromatography with nickel ion coated beads. Both enzyme preparations were characterised to determine basic kinetic parameters, the ability to degrade cation-alginate gels and tolerance to thermal degradation. Enzyme activity was measured using UV spectroscopy and rheometry based assays. Following enzyme characterisation biofilms were grown both in multi-well plates and on coupons, and were treated with two different alginate lyases along with carbenicillin and gentamicin antibiotics. Biofilm imaging techniques using a LIVE/DEAD cell viability stain to determine if lyases affected biofilm cell viability were trialled unsuccessfully. Alginate lyase, at the concentrations used in the study did not increase antibiotic sensitivity. Treatment with alginate lyase either had no effect on biofilm populations or caused a small but significant increase in viable cell number and overall biomass. The results suggested that the biofilm populations… Advisors/Committee Members: Lamont, Iain (advisor).

Subjects/Keywords: Alginate; Lyase; Biofilms; Pseudomonas

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APA (6^th Edition):

Germoni, L. A. P. (2014). The effect of alginate lyase on the antibiotic sensitivity of mucoid Pseudomonas aeruginosa biofilms . (Doctoral Dissertation). University of Otago. Retrieved from http://hdl.handle.net/10523/4784

Chicago Manual of Style (16^th Edition):

Germoni, Leonardo Alexandro Piero. “The effect of alginate lyase on the antibiotic sensitivity of mucoid Pseudomonas aeruginosa biofilms .” 2014. Doctoral Dissertation, University of Otago. Accessed August 22, 2019. http://hdl.handle.net/10523/4784.

MLA Handbook (7^th Edition):

Germoni, Leonardo Alexandro Piero. “The effect of alginate lyase on the antibiotic sensitivity of mucoid Pseudomonas aeruginosa biofilms .” 2014. Web. 22 Aug 2019.

Vancouver:

Germoni LAP. The effect of alginate lyase on the antibiotic sensitivity of mucoid Pseudomonas aeruginosa biofilms . [Internet] [Doctoral dissertation]. University of Otago; 2014. [cited 2019 Aug 22]. Available from: http://hdl.handle.net/10523/4784.

Council of Science Editors:

Germoni LAP. The effect of alginate lyase on the antibiotic sensitivity of mucoid Pseudomonas aeruginosa biofilms . [Doctoral Dissertation]. University of Otago; 2014. Available from: http://hdl.handle.net/10523/4784

Penn State University

5. Lalpuria, Minal Prakash. Mechanisms and kinetics of nisin release from calcium alginate films.

Degree: PhD, Food Science, 2012, Penn State University

URL: https://etda.libraries.psu.edu/catalog/14660

► Several studies have shown that calcium alginate matrices containing nisin effectively inhibits microbial growth in various foods such as beef, poultry, milk, and mushrooms, but… (more)

▼ Several studies have shown that calcium alginate matrices containing nisin effectively inhibits microbial growth in various foods such as beef, poultry, milk, and mushrooms, but they only present qualitative information on overall effectiveness of these systems on microbial inhibition. In order to design an effective antimicrobial packaging system for commercial applications, it is important to study the impact of various factors that govern the structure and properties of calcium alginate films and the diffusion of nisin from these films. It is known that nisin is a cationic polypeptide, whereas alginate is an anionic polymer. Ionic interactions between nisin and alginate might hamper the release of nisin from the alginate matrix, leading to reduced antimicrobial activity. Hence, it is essential to characterize interactions between nisin and alginate and to investigate the antimicrobial activity of nisin released from calcium alginate films. The overall goal of this research was to study the mechanisms and kinetics of nisin release from calcium alginate films. The objectives were to: (a) modify agar diffusion assay for better quantification of nisin; (b) determine factors affecting the release of nisin from calcium alginate films; (c) investigate the interaction between nisin and alginate; (d) determine the kinetics of release; (e) evaluate the physical, mechanical and optical properties of these films; and (f) investigate antimicrobial activity of the released nisin. Agar diffusion bioassay, using Micrococcus luteus, is a commonly used method for quantification of nisin concentration. However, the correlation between inhibition zone and nisin concentration is not well established at higher nisin concentrations. The objective of this study was to investigate the effect of different well size (small and large) and pre-diffusion times (0, 24, 48 and 72 h) at 4°C on the sensitivity, precision and accuracy of nisin quantification. Regression analysis indicate that small wells were better than large wells because of smaller standard deviation, higher predictive accuracy, and better discrimination between mean inhibition zones at neighboring nisin concentrations. Statistical analysis by Tukey’s test showed that pre-diffusion resulted in significantly different inhibition zones at different nisin concentations. Pre-diffusion also improved sensitivity of the assay at different nisin concentrations. Different regression models were considered to explore the relationship between inhibition zone and nisin concentration for all pre-diffusion times. A spline model was the best fit model and 48 h was the best pre-diffusion time. Factors affecting the release of nisin from calcium alginate films were studied by the disc agar diffusion bioassay. Calcium alginate films were formed by two methods – cross-linking before drying (CBD) and cross-linking after drying (CAD) – using various alginate compositions, cross-linking times and Ca2+concentrations. The method of film formation, cross-linking time, and Ca2+concentration had a…

Subjects/Keywords: antimicrobial packaging; nisin; alginate; diffusion

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APA (6^th Edition):

Lalpuria, M. P. (2012). Mechanisms and kinetics of nisin release from calcium alginate films. (Doctoral Dissertation). Penn State University. Retrieved from https://etda.libraries.psu.edu/catalog/14660

Chicago Manual of Style (16^th Edition):

Lalpuria, Minal Prakash. “Mechanisms and kinetics of nisin release from calcium alginate films.” 2012. Doctoral Dissertation, Penn State University. Accessed August 22, 2019. https://etda.libraries.psu.edu/catalog/14660.

MLA Handbook (7^th Edition):

Lalpuria, Minal Prakash. “Mechanisms and kinetics of nisin release from calcium alginate films.” 2012. Web. 22 Aug 2019.

Vancouver:

Lalpuria MP. Mechanisms and kinetics of nisin release from calcium alginate films. [Internet] [Doctoral dissertation]. Penn State University; 2012. [cited 2019 Aug 22]. Available from: https://etda.libraries.psu.edu/catalog/14660.

Council of Science Editors:

Lalpuria MP. Mechanisms and kinetics of nisin release from calcium alginate films. [Doctoral Dissertation]. Penn State University; 2012. Available from: https://etda.libraries.psu.edu/catalog/14660

Massey University

6. Ren, Lu. Production of alginate beads : a project report [i.e. thesis] presented in partial fulfillment of the requirements for the degree of Master in Food Technology at Massey University, Auckland, New Zealand.

Degree: 2010, Massey University

URL: http://hdl.handle.net/10179/1192

► This paper was to improve the production of calcium-induced alginate gels manufactured by a company in Auckland. Problems encountered included yield and syneresis of the… (more)

Subjects/Keywords: Alginates; Gelation; Alginate gel beads

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APA (6^th Edition):

Ren, L. (2010). Production of alginate beads : a project report [i.e. thesis] presented in partial fulfillment of the requirements for the degree of Master in Food Technology at Massey University, Auckland, New Zealand. (Thesis). Massey University. Retrieved from http://hdl.handle.net/10179/1192

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ren, Lu. “Production of alginate beads : a project report [i.e. thesis] presented in partial fulfillment of the requirements for the degree of Master in Food Technology at Massey University, Auckland, New Zealand. ” 2010. Thesis, Massey University. Accessed August 22, 2019. http://hdl.handle.net/10179/1192.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ren, Lu. “Production of alginate beads : a project report [i.e. thesis] presented in partial fulfillment of the requirements for the degree of Master in Food Technology at Massey University, Auckland, New Zealand. ” 2010. Web. 22 Aug 2019.

Vancouver:

Ren L. Production of alginate beads : a project report [i.e. thesis] presented in partial fulfillment of the requirements for the degree of Master in Food Technology at Massey University, Auckland, New Zealand. [Internet] [Thesis]. Massey University; 2010. [cited 2019 Aug 22]. Available from: http://hdl.handle.net/10179/1192.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ren L. Production of alginate beads : a project report [i.e. thesis] presented in partial fulfillment of the requirements for the degree of Master in Food Technology at Massey University, Auckland, New Zealand. [Thesis]. Massey University; 2010. Available from: http://hdl.handle.net/10179/1192

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Ottawa

7. Khan, Brandon Sajad. The Effects of Gamma (γ-) Sterilization on the Redox Stability, Minerology, and Physicochemical Properties of the Synthetic Iron Oxides Ferrihydrite, Lepidocrocite, and Goethite .

Degree: 2017, University of Ottawa

URL: http://hdl.handle.net/10393/36519

► Laboratory analyses were conducted on synthetic iron oxides to assess the use of gamma (γ-) irradiation as an efficient sterilization technique to remove microorganisms present… (more)

Subjects/Keywords: BIOS; Fe-oxides; Sterilization; Alginate

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APA (6^th Edition):

Khan, B. S. (2017). The Effects of Gamma (γ-) Sterilization on the Redox Stability, Minerology, and Physicochemical Properties of the Synthetic Iron Oxides Ferrihydrite, Lepidocrocite, and Goethite . (Thesis). University of Ottawa. Retrieved from http://hdl.handle.net/10393/36519

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Khan, Brandon Sajad. “The Effects of Gamma (γ-) Sterilization on the Redox Stability, Minerology, and Physicochemical Properties of the Synthetic Iron Oxides Ferrihydrite, Lepidocrocite, and Goethite .” 2017. Thesis, University of Ottawa. Accessed August 22, 2019. http://hdl.handle.net/10393/36519.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Khan, Brandon Sajad. “The Effects of Gamma (γ-) Sterilization on the Redox Stability, Minerology, and Physicochemical Properties of the Synthetic Iron Oxides Ferrihydrite, Lepidocrocite, and Goethite .” 2017. Web. 22 Aug 2019.

Vancouver:

Khan BS. The Effects of Gamma (γ-) Sterilization on the Redox Stability, Minerology, and Physicochemical Properties of the Synthetic Iron Oxides Ferrihydrite, Lepidocrocite, and Goethite . [Internet] [Thesis]. University of Ottawa; 2017. [cited 2019 Aug 22]. Available from: http://hdl.handle.net/10393/36519.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Khan BS. The Effects of Gamma (γ-) Sterilization on the Redox Stability, Minerology, and Physicochemical Properties of the Synthetic Iron Oxides Ferrihydrite, Lepidocrocite, and Goethite . [Thesis]. University of Ottawa; 2017. Available from: http://hdl.handle.net/10393/36519

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Texas – Austin

8. Mayes, Sarah Margaret. Hyaluronic acid and alginate blend hydrogel films for the prevention of postsurgical adhesions.

Degree: Biomedical Engineering, 2013, University of Texas – Austin

URL: http://hdl.handle.net/2152/22058

► Postoperative adhesions form as the body's natural response to injury in an effort to temporarily protect and supply nutrients to these tissues. However, adhesions can… (more)

▼ Postoperative adhesions form as the body's natural response to injury in an effort to temporarily protect and supply nutrients to these tissues. However, adhesions can remain permanent, and fail otherwise successful surgeries by tethering tissues together that are normally separated. An ideal anti-adhesive device reduces unwanted adhesions and leaves the patient in a state most similar to before surgery. This dissertation introduces a novel, robust hydrogel film consisting of two hydrophilic polydisaccharides, hyaluronic acid (HA) and alginate. To address the challenge of retaining HA in alginate-rich hydrogels, we methacrylated the HA (GMHA), creating hydrophobic moieties. These hydrophobic interactions shift the percolation threshold, allowing for greater concentrations of GMHA to be retained in resulting films. UV crosslinking retains GMHA beyond the percolation threshold and widens the possibilities of usable films. To enhance the mechanical properties of these alginate/GMHA films, we employed a previously developed method for creating thin, branched, interconnected fibers using urea crystal templating. Templated films are softer and, yet, tougher than films that have not been templated. This toughness is a result of increased density of polymer in the fibers. These films were selected as most conformable and most robust by surgeons in a blinded handling study. In a rat peritoneal abrasion model for adhesion formation, the films successfully prevented adhesions with statistical equivalence to the leading anti-adhesion device commercially available. Finally, future recommendations are suggested for the development of a bilayer construct with a collagen/alginate blend bound to an alginate/HA layer for an anti-adhesive and regenerative strategy. This construct addresses the need for opposing strategies in the dynamic environment of wound healing. Further research is needed to develop the usefulness of this bilayer system, as preventing unwanted adhesions is merely a first step in achieving a blemish-free healed wound. Advisors/Committee Members: Stachowiak, Jeanne Casstevens (advisor), Schmidt, Christine E. (advisor).

Subjects/Keywords: Hyaluronic acid; Alginate; Abdominal adhesions

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APA (6^th Edition):

Mayes, S. M. (2013). Hyaluronic acid and alginate blend hydrogel films for the prevention of postsurgical adhesions. (Thesis). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/22058

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mayes, Sarah Margaret. “Hyaluronic acid and alginate blend hydrogel films for the prevention of postsurgical adhesions.” 2013. Thesis, University of Texas – Austin. Accessed August 22, 2019. http://hdl.handle.net/2152/22058.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mayes, Sarah Margaret. “Hyaluronic acid and alginate blend hydrogel films for the prevention of postsurgical adhesions.” 2013. Web. 22 Aug 2019.

Vancouver:

Mayes SM. Hyaluronic acid and alginate blend hydrogel films for the prevention of postsurgical adhesions. [Internet] [Thesis]. University of Texas – Austin; 2013. [cited 2019 Aug 22]. Available from: http://hdl.handle.net/2152/22058.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mayes SM. Hyaluronic acid and alginate blend hydrogel films for the prevention of postsurgical adhesions. [Thesis]. University of Texas – Austin; 2013. Available from: http://hdl.handle.net/2152/22058

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Connecticut

9. Podany, Michael V, Mr. Investigation of In-situ Immobilization of Contaminated Sediments using Alginate Gels for the Reduction of Ecological Risk.

Degree: MS, Environmental Engineering, 2012, University of Connecticut

URL: https://opencommons.uconn.edu/gs_theses/371

► Remediation methods for contaminated sediments include dredging, treatment and disposal and sediment isolation through capping. These methods are costly and adversely impact local ecosystems,… (more)

Subjects/Keywords: alginate; sediment; remediation; in-situ

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APA (6^th Edition):

Podany, Michael V, M. (2012). Investigation of In-situ Immobilization of Contaminated Sediments using Alginate Gels for the Reduction of Ecological Risk. (Masters Thesis). University of Connecticut. Retrieved from https://opencommons.uconn.edu/gs_theses/371

Chicago Manual of Style (16^th Edition):

Podany, Michael V, Mr. “Investigation of In-situ Immobilization of Contaminated Sediments using Alginate Gels for the Reduction of Ecological Risk.” 2012. Masters Thesis, University of Connecticut. Accessed August 22, 2019. https://opencommons.uconn.edu/gs_theses/371.

MLA Handbook (7^th Edition):

Podany, Michael V, Mr. “Investigation of In-situ Immobilization of Contaminated Sediments using Alginate Gels for the Reduction of Ecological Risk.” 2012. Web. 22 Aug 2019.

Vancouver:

Podany, Michael V M. Investigation of In-situ Immobilization of Contaminated Sediments using Alginate Gels for the Reduction of Ecological Risk. [Internet] [Masters thesis]. University of Connecticut; 2012. [cited 2019 Aug 22]. Available from: https://opencommons.uconn.edu/gs_theses/371.

Council of Science Editors:

Podany, Michael V M. Investigation of In-situ Immobilization of Contaminated Sediments using Alginate Gels for the Reduction of Ecological Risk. [Masters Thesis]. University of Connecticut; 2012. Available from: https://opencommons.uconn.edu/gs_theses/371

University of Saskatchewan

10. Yasmin, Farhana 1990-. DEVELOPMENT OF ALGINATE NANOSPHERES AS A PROTEIN DELIVERY DEVICE FOR CARTILAGE TISSUE ENGINEERING.

Degree: 2018, University of Saskatchewan

URL: http://hdl.handle.net/10388/11546

► Delivery of bioactive proteins is a valuable strategy in cartilage tissue engineering (CTE) because of their ability to regulate the gene expression and extracellular matrix… (more)

▼ Delivery of bioactive proteins is a valuable strategy in cartilage tissue engineering (CTE) because of their ability to regulate the gene expression and extracellular matrix (ECM) production of engineered cartilage. This, however, has been challenged by the nature of bioactive proteins including their instability, poor tissue penetration ability, short half-life and a relatively high price. Development of nanospheres as a protein delivery device should solve these issues by promoting the temporal and spatial presentation of such bioactive proteins in a defined target for the enhanced half-life time and effectiveness. Among various polymer-based micro/nanospheres, alginate micro/nanospheres have been widely used as a protein delivery device because of their mild and easy protein encapsulation process, inert nature, non-toxicity and biocompatibility. However, one of the major limitations of using alginate as a protein delivery device is its high initial burst release due to its high porosity and instability if exposed in a higher pH release media. To address these issues, this study aimed to develop the protein loaded alginate nanospheres as a delivery device with a reduced initial burst release. The hypothesis was, “Increasing the alginate concentration, cross-linking time or drying time reduces the initial burst release independently of associated changes to the size and number of nanospheres.” Bovine Serum Albumin (BSA) was used as a model protein in this study to evaluate the performance of alginate nanospheres as a protein delivery device, while protein loaded alginate nanospheres were prepared via a combination of water-in-oil emulsification and external gelation method. The process parameters tested to reduce the initial burst release include, alginate concentration, cross-linking time and drying time. The effects of these process parameters on the nanosphere size and distribution pattern, relative number of microspheres, initial burst release, protein release kinetics and encapsulation efficiency (EE%) were investigated. Also, if the change in size and relative number of nanospheres by varying these process parameters affected the initial burst release was investigated. It has been illustrated that by properly increasing the alginate concentration, cross-linking time and drying time it was possible to reduce initial burst release by 13%, and among various process parameters only the alginate concentration showed a significant effect on the initial burst release, when considered alone. Also, it was confirmed that during determination of the effect of various process parameters the relative number of nanospheres significantly affected the initial burst release. Taken together, this study demonstrates that regulating various process parameters is a mean to reduce initial burst release of alginate nanospheres, urging more studies on alginate nanospheres for their potential application in CTE. Advisors/Committee Members: Chen, Daniel, Eames, Brian, Zhu, Ning, Boughner, Julia, Wasan, Ellen.

Subjects/Keywords: alginate nanospheres; protein delivery

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APA (6^th Edition):

Yasmin, F. 1. (2018). DEVELOPMENT OF ALGINATE NANOSPHERES AS A PROTEIN DELIVERY DEVICE FOR CARTILAGE TISSUE ENGINEERING. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/11546

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yasmin, Farhana 1990-. “DEVELOPMENT OF ALGINATE NANOSPHERES AS A PROTEIN DELIVERY DEVICE FOR CARTILAGE TISSUE ENGINEERING.” 2018. Thesis, University of Saskatchewan. Accessed August 22, 2019. http://hdl.handle.net/10388/11546.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yasmin, Farhana 1990-. “DEVELOPMENT OF ALGINATE NANOSPHERES AS A PROTEIN DELIVERY DEVICE FOR CARTILAGE TISSUE ENGINEERING.” 2018. Web. 22 Aug 2019.

Vancouver:

Yasmin F1. DEVELOPMENT OF ALGINATE NANOSPHERES AS A PROTEIN DELIVERY DEVICE FOR CARTILAGE TISSUE ENGINEERING. [Internet] [Thesis]. University of Saskatchewan; 2018. [cited 2019 Aug 22]. Available from: http://hdl.handle.net/10388/11546.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yasmin F1. DEVELOPMENT OF ALGINATE NANOSPHERES AS A PROTEIN DELIVERY DEVICE FOR CARTILAGE TISSUE ENGINEERING. [Thesis]. University of Saskatchewan; 2018. Available from: http://hdl.handle.net/10388/11546

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Duquesne University

11. Fu, Shao. Inter-Grade and Inter-Batch Variability of Pharmaceutical-Grade Sodium Alginate.

Degree: PhD, Pharmaceutics, 2011, Duquesne University

URL: https://dsc.duq.edu/etd/557

► Polymeric excipients are generally the least well-characterized components of pharmaceutical formulations. The aim of this dissertation work is to facilitate the quality-by-design (QbD) approach to… (more)

▼ Polymeric excipients are generally the least well-characterized components of pharmaceutical formulations. The aim of this dissertation work is to facilitate the quality-by-design (QbD) approach to pharmaceutical formulation and manufacturing by evaluating the inter-grade and inter-batch variability of pharmaceutical-grade polymeric excipients. Sodium alginate, a widely used polymeric excipient, was selected for evaluation using appropriate analytical methods and test conditions, especially rheological methods. The materials used were six different grades of sodium alginate and an additional ten batches of one of the grades. To compare the six grades, steady shear measurements were conducted on solutions at 1, 2, and 3% w/w, consistent with their use as thickening or binding agents. Small amplitude oscillation (SAO) measurements were conducted on sodium alginate solutions at higher concentrations (4-13% w/w) corresponding to their use in controlled release matrices. In order to compare the ten batches of one grade, steady shear and SAO measurements were performed on their solutions at 2% w/w and 8% w/w, respectively. Results show that rheological properties of sodium alginate solutions are influenced by both molecular weight and chemical composition of sodium alginate. ¡§One-point¡¨ apparent viscosity data obtained at one low concentration and one shear rate is not representative of the complex rheological behavior of various grades of sodium alginate solutions at higher concentrations or other shear rates. The potential interchangeability of these different grades used as thickening or binding agents could be established by comparing the apparent viscosities of their solutions as a function of both alginate concentration and shear conditions. For sodium alginate used in controlled release formulations, both steady shear (at one low concentration, e.g., 2% w/w) and SAO measurements (at one high concentration indicative of polymer gel state, e.g., 8% w/w) are recommended to be performed on sodium alginate solutions to ensure interchangeability. Furthermore, among batches of the same grade, significant differences in rheological properties were observed, especially at the high solution concentration (i.e., 8% w/w). In summary, inter-grade and inter-batch variability of sodium alginate can be determined using steady shear and SAO methods. The influence of inter-grade and inter-batch variability of sodium alginate on the functionality of sodium alginate used in matrix tablets was investigated with a focus on compression properties, swelling, erosion behavior of alginate matrix tablets, and drug release from matrix tablets. The compression behavior of four grades and three batches of sodium alginate were studied by compaction energetics, out-of-die Gurnham, and out-of-die Heckel analysis. It was found that sodium alginates deform less plastically than microcrystalline cellulose (MCC PH102) but similar to lactose anhydrous. Sodium alginates also demonstrate more elastic deformations during compression than… Advisors/Committee Members: Lawrence H. Block, Peter Wildfong, Ira Buckner, Brian Carlin, Sachin Velankar, Christianah Moji Adeyeye.

Subjects/Keywords: Calcium alginate gels; Controlled release matrix; Rheology; Sodium alginate; Viscoelasticity; Viscosity

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APA (6^th Edition):

Fu, S. (2011). Inter-Grade and Inter-Batch Variability of Pharmaceutical-Grade Sodium Alginate. (Doctoral Dissertation). Duquesne University. Retrieved from https://dsc.duq.edu/etd/557

Chicago Manual of Style (16^th Edition):

Fu, Shao. “Inter-Grade and Inter-Batch Variability of Pharmaceutical-Grade Sodium Alginate.” 2011. Doctoral Dissertation, Duquesne University. Accessed August 22, 2019. https://dsc.duq.edu/etd/557.

MLA Handbook (7^th Edition):

Fu, Shao. “Inter-Grade and Inter-Batch Variability of Pharmaceutical-Grade Sodium Alginate.” 2011. Web. 22 Aug 2019.

Vancouver:

Fu S. Inter-Grade and Inter-Batch Variability of Pharmaceutical-Grade Sodium Alginate. [Internet] [Doctoral dissertation]. Duquesne University; 2011. [cited 2019 Aug 22]. Available from: https://dsc.duq.edu/etd/557.

Council of Science Editors:

Fu S. Inter-Grade and Inter-Batch Variability of Pharmaceutical-Grade Sodium Alginate. [Doctoral Dissertation]. Duquesne University; 2011. Available from: https://dsc.duq.edu/etd/557

Anna University

12. Praveena Juliya Dorathi R. Degradation of chlorophenols by sonication coupled ferrate and ferrous alginate and dechlorination by iron immobilized silica;.

Degree: 2014, Anna University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/14691

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Due to industrialization and urbanization, rapid depletion of potable water and pollution of water bodies are taking place. Grossly, polluted water has hindered the ability… (more)

▼

Due to industrialization and urbanization, rapid depletion of potable water and pollution of water bodies are taking place. Grossly, polluted water has hindered the ability of nature to revive and sustain itself. This scenario had led to the formation of stringent environmental regulatory laws. Hence, researchers worldwide over are trying to develop new techniques which are effective and economical for the degradation of the recalcitrants. Chlorophenols are mainly used as disinfectants, biocides, preservatives, pesticides and in the manufacturing of other chlorinated chemicals. Experiments were conducted using +6, +2 and 0 oxidation state ofiron to degrade chlorophenols in innovative ways. The drawback of using ferrate is that it is stable only at high alkaline pH, requirement of high ferate : chlorophenol ratio, use of buffers in degradation study (not suitable for waste water) and storage. Alginate, a polymer obtained from natural source (brown algae) has been used as support material for ferrous immobilization in this research. It was coupled with sonication for better efficiency. Ferrous alginate beads were prepared using a simple protocol. It was found to be stable at pH 2, which is an added advantage as the optimal pH for Fenton s method is 2 3. The order of degradation among chlorophenols was CPgt DCPgt TCP and the degradation followed pseudo first order kinetics. Immobilization of Fe0 (ZVI) is also a topic of research for many researchers. Granular silica, which is easily available, has been experimented as a support material for ZVI. The indigenously prepared ZVI immobilized silica was found to be effective in dechlorinating the chlorophenols. The optimal pH, column height and flow rate was found to be 7, 20 cm and 0.75L/h respectively.The order of dechlorination was TCPgt DCPgt CP. All the methods developed in this study were applied to real pulp and paper industrial wastewater. The most economically viable and efficient method among them was sono heterogeneous Fenton method. newline newline newline

Appendix 1, pp.170-172

Advisors/Committee Members: Palanivelu, K..

Subjects/Keywords: Chlorophenols; dechlorination; ferrous alginate; immobilized silica

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APA (6^th Edition):

R, P. J. D. (2014). Degradation of chlorophenols by sonication coupled ferrate and ferrous alginate and dechlorination by iron immobilized silica;. (Thesis). Anna University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/14691

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

R, Praveena Juliya Dorathi. “Degradation of chlorophenols by sonication coupled ferrate and ferrous alginate and dechlorination by iron immobilized silica;.” 2014. Thesis, Anna University. Accessed August 22, 2019. http://shodhganga.inflibnet.ac.in/handle/10603/14691.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

R, Praveena Juliya Dorathi. “Degradation of chlorophenols by sonication coupled ferrate and ferrous alginate and dechlorination by iron immobilized silica;.” 2014. Web. 22 Aug 2019.

Vancouver:

R PJD. Degradation of chlorophenols by sonication coupled ferrate and ferrous alginate and dechlorination by iron immobilized silica;. [Internet] [Thesis]. Anna University; 2014. [cited 2019 Aug 22]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/14691.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

R PJD. Degradation of chlorophenols by sonication coupled ferrate and ferrous alginate and dechlorination by iron immobilized silica;. [Thesis]. Anna University; 2014. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/14691

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Univerzitet u Beogradu

13. Lević, Steva M., 1981-. Inkapsulacija aroma u karnauba vosku, alginatu i polivinil-alkoholu.

Degree: Poljoprivredni fakultet, 2016, Univerzitet u Beogradu

URL: https://fedorabg.bg.ac.rs/fedora/get/o:11100/bdef:Content/get

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Biotehničke nauke - Nauka o konzervisanju / Biotechnical sciences - The science of food preservation

U ovoj disertaciji je ispitivana inkapsulacija prehrambenih aroma uz primenu… (more)

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Biotehničke nauke - Nauka o konzervisanju / Biotechnical sciences - The science of food preservation

U ovoj disertaciji je ispitivana inkapsulacija prehrambenih aroma uz primenu različitih metoda inkapsulacije i u kombinaciji sa prirodnim i sintetičkim nosačima. Cilj istraživanja je bio dobijanje inkapsulata kako tečnih tako i čvrstih aroma koje se najčešće koriste u prehrambenoj industriji. Ispitivana su tri nosača za inkapsulaciju aroma: karnauba vosak, alginat i polivinil alkohol. Kao model arome su korišćene: etil vanilin, komercijalno dostupna aroma kokosa i D-limonen. Karnauba vosak je korišćen kao nosač za inkapsulaciju etil vanilina, pri čemu su dobijene sferne čestice čiji je prečnik uglavnom bio ispod 250μm. Čestice na bazi karnauba voska sa etil vanilinom su dobijene primenom disperzione (emulzifikacione) metode i disperzione tehnike (disperzija komprimovanim vazduhom). Metodom disperzije vazduhom su dobijene čestice pravilnijeg sfernog oblika sa većom inkapsulacionom efikasnošću. Takođe, termička svojstva inkapsulisanog etil vanilina su poboljšana u poređenju sa slobodnom aromom. Termičko otpuštanje inkapsulisanog etil vanilina se odigravalo na višim temperaturama (do~270°C) u poređenju sa slobonom aromom (do ~220°C). Ca-alginatne čestice sa tečnim i čvrstim aromama su dobijane metodom elektrostatičke ekstruzije. Inkapsulacijom etil vanilina u Ca-alginatni matriks su ostvarena bolja termička svojstva inkapsulisane arome uz postizanje kontrolisanog termičkog otpuštanja arome u širem temperaturnom opsegu u odnosu na slobodnu aromu. Aroma kokosa i D-limonen su inkapsulisani u Ca-alginatne čestice primenom iste procedure kao i u slučaju inkapsulacije etil vanilina. Alginat je pokazao dobra emulgujuća svojstva, što je osnovni uslov za postizanje visoke inkapsulacione efikasnosti. Rezultati termičke analize su pokazali da obe arome u inkapsulisanom obliku imaju bolja termička svojstva u odnosu na slobodne arome. Rehidracija je korišćena za ispitivanje adsorpcije vode u inkapsulate uz razvoj novog modela rehidracije. Polivinil alkohol je u kombinaciji sa alginatom korišćen za dobijanje čestica sa inkapsulisanim etil vanilinom metodom elektrostatičke ekstruzije. Primenom viših napona kod metode elektrostatičke ekstruzije-elektrospininga dobijeni su filmovi na bazi polivinil alkohola koji se sastoje od nanovlakana. Filmovi na bazi polivinil alkohola su pokazali dobra hemijska, mehanička, termička i antimikrobna svojstva...

Advisors/Committee Members: Nedović, Viktor, 1964-.

Subjects/Keywords: encapsulation; carnauba wax; alginate; polyvinyl alcohol; flavours

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lević, Steva M., 1. (2016). Inkapsulacija aroma u karnauba vosku, alginatu i polivinil-alkoholu. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:11100/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lević, Steva M., 1981-. “Inkapsulacija aroma u karnauba vosku, alginatu i polivinil-alkoholu.” 2016. Thesis, Univerzitet u Beogradu. Accessed August 22, 2019. https://fedorabg.bg.ac.rs/fedora/get/o:11100/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lević, Steva M., 1981-. “Inkapsulacija aroma u karnauba vosku, alginatu i polivinil-alkoholu.” 2016. Web. 22 Aug 2019.

Vancouver:

Lević, Steva M. 1. Inkapsulacija aroma u karnauba vosku, alginatu i polivinil-alkoholu. [Internet] [Thesis]. Univerzitet u Beogradu; 2016. [cited 2019 Aug 22]. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:11100/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lević, Steva M. 1. Inkapsulacija aroma u karnauba vosku, alginatu i polivinil-alkoholu. [Thesis]. Univerzitet u Beogradu; 2016. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:11100/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Univerzitet u Beogradu

14. Prodanović, Olivera L. 1973-. Razvoj imobilisanih sistema peroksidaze iz rena (Armoracia rusticana) za polimerizacione reakcije i uklanjanje fenola iz otpadnih voda.

Degree: Tehnološko-metalurški fakultet, 2016, Univerzitet u Beogradu

URL: https://fedorabg.bg.ac.rs/fedora/get/o:11259/bdef:Content/get

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Biotehnologija i biohemijsko inženjerstvo / Biotechnology and Biochemical Engineering

U prvom delu ove teze je makroporozni kopolimer glicidil metakrilata i etilen glikol dimetakrilata, poly(GMA-co-EGDMA) sa… (more)

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Biotehnologija i biohemijsko inženjerstvo / Biotechnology and Biochemical Engineering

U prvom delu ove teze je makroporozni kopolimer glicidil metakrilata i etilen glikol dimetakrilata, poly(GMA-co-EGDMA) sa dijametrom čestica od 150 do 500 μm i različitim karakteristikama površine i različitom srednjom veličinom dijametra pora od 44 do 200 nm sintetisan, modifikovan 1,2-diaminoetanom i testiran kao nosač za imobilizaciju peroksidaze iz rena (HRP) koristeći dve kovalentne metode, glutaraldehidnu i perjodatnu. Najveća specifična aktivnost od oko 35 U/g suve mase nosača je postignuta na poly(GMA-co-EGDMA) kopolimeru sa srednjom veličinom dijametra pora od 200 i 120 nm koristeći perjodatni metod. Proučavanjem kinetike deaktivacije na 65 °C i u 80% (v/v) dioksanu je otkriveno da perjodatna metoda takođe dovodi do značajne stabilizacije biokatalizatora, i da stabilizacioni faktor zavisi snažno od površinskih karakteristika kopolimera. HRP imobilizovana na kopolimeru sa srednjom veličinom dijametra pora od 120 nm perjodatnom metodom je pokazala najveću specifičnu aktivnost ali takođe i dobru stabilnost, tako da je u daljem radu dodatno karakterisana. Imobilizacija je dovela do stabilizacije enzima u širem opsegu pH, dok je Mihaelisova konstanta (Km) imobilizovane HRP bila 10,8 mM što je otprilike 5,6 puta više nego za slobodni enzim. Posle 6 upotreba u bač reaktoru za oksidaciju pirogalola imobilizovana peroksidaza je zadržala 45% polazne aktivnosti. U drugom delu ove teze ista serija makroporoznih kopolimera glicidil metakrilata i etilen glikol dimetakrilata, poly(GMA-co-EGDMA) sa veličinom pora od 200 nm (ZS9) pa do neporoznog (ZS6) je testirana kao nosač za imobilizaciju peroksidaze iz rena, ali ovaj put sa dijametrom čestica od 2 do 2,5 μm. HRP je imobilizovana na 4 načina, preko epoksidne grupe na kopolimeru, i nakon aminovanja kopolimera glutaraldehidnom, perjodatnom i karbodiimidnom metodom. Najveća aktivnost imobilizovane HRP od 14,5 U/g je dobijena glutaraldehidnom metodom, a odmah zatim najveća aktivnost od 13,7 U/g karbodiimidnom metodom na poly(GMA-co-EGDMA) označenom kao ZS9 čija je srednja veličina pora bila oko 200 nm. Prinos imobilizacije je bio veći u odnosu na seriju kopolimera sa većom veličinom čestica i iznosio je 33,5% za glutaraldehidnu metodu i 30,3 % za karbodiimidnu u odnosu na oko 5% prinosa dobijenog za seriju kopolimera sa većom veličinom čestica i...

Advisors/Committee Members: Knežević-Jugović, Zorica..

Subjects/Keywords: peroxidase; macroporous; alginate; emulsion; tyramine; phenol

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APA (6^th Edition):

Prodanović, O. L. 1. (2016). Razvoj imobilisanih sistema peroksidaze iz rena (Armoracia rusticana) za polimerizacione reakcije i uklanjanje fenola iz otpadnih voda. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:11259/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Prodanović, Olivera L 1973-. “Razvoj imobilisanih sistema peroksidaze iz rena (Armoracia rusticana) za polimerizacione reakcije i uklanjanje fenola iz otpadnih voda.” 2016. Thesis, Univerzitet u Beogradu. Accessed August 22, 2019. https://fedorabg.bg.ac.rs/fedora/get/o:11259/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Prodanović, Olivera L 1973-. “Razvoj imobilisanih sistema peroksidaze iz rena (Armoracia rusticana) za polimerizacione reakcije i uklanjanje fenola iz otpadnih voda.” 2016. Web. 22 Aug 2019.

Vancouver:

Prodanović OL1. Razvoj imobilisanih sistema peroksidaze iz rena (Armoracia rusticana) za polimerizacione reakcije i uklanjanje fenola iz otpadnih voda. [Internet] [Thesis]. Univerzitet u Beogradu; 2016. [cited 2019 Aug 22]. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:11259/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Prodanović OL1. Razvoj imobilisanih sistema peroksidaze iz rena (Armoracia rusticana) za polimerizacione reakcije i uklanjanje fenola iz otpadnih voda. [Thesis]. Univerzitet u Beogradu; 2016. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:11259/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

15. Quyen, Nguyen. Core-Shell Alginate Microgles with an Electro-Responsive Movement.

Degree: Japan Advanced Institute of Science and Technology / 北陸先端科学技術大学院大学

URL: http://hdl.handle.net/10119/8357

Supervisor:Associate Professor TATSUO KANEKO

マテリアルサイエンス研究科

修士

Subjects/Keywords: Alginate; Electro-Responsive

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Quyen, N. (n.d.). Core-Shell Alginate Microgles with an Electro-Responsive Movement. (Thesis). Japan Advanced Institute of Science and Technology / 北陸先端科学技術大学院大学. Retrieved from http://hdl.handle.net/10119/8357

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Quyen, Nguyen. “Core-Shell Alginate Microgles with an Electro-Responsive Movement.” Thesis, Japan Advanced Institute of Science and Technology / 北陸先端科学技術大学院大学. Accessed August 22, 2019. http://hdl.handle.net/10119/8357.

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Quyen, Nguyen. “Core-Shell Alginate Microgles with an Electro-Responsive Movement.” Web. 22 Aug 2019.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

Quyen N. Core-Shell Alginate Microgles with an Electro-Responsive Movement. [Internet] [Thesis]. Japan Advanced Institute of Science and Technology / 北陸先端科学技術大学院大学; [cited 2019 Aug 22]. Available from: http://hdl.handle.net/10119/8357.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

Council of Science Editors:

Quyen N. Core-Shell Alginate Microgles with an Electro-Responsive Movement. [Thesis]. Japan Advanced Institute of Science and Technology / 北陸先端科学技術大学院大学; Available from: http://hdl.handle.net/10119/8357

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

Penn State University

16. Chandrasekar, Vaishnavi. Release kinetics of nisin from chitosan-alginate films and microparticles.

Degree: PhD, Food Science, 2015, Penn State University

URL: https://etda.libraries.psu.edu/catalog/26302

► Antimicrobials are being used to inhibit the growth of pathogenic and spoilage bacteria, thereby enhancing safety and extending the shelf-life of processed foods. Nisin is… (more)

▼ Antimicrobials are being used to inhibit the growth of pathogenic and spoilage bacteria, thereby enhancing safety and extending the shelf-life of processed foods. Nisin is a bacteriocin that has Generally Recognized as Safe (GRAS) status, and is effective against Gram positive bacteria. Studies have shown that when nisin is directly applied to the surface of foods, it loses its biological activity over time. This loss is due to binding interactions with proteins and fats, and degradation by proteolytic enzymes, as well as due to diffusion into the bulk of the food. Delivery systems with sustained release properties of nisin have the potential to improve its stability and biological functionality in foods. Chitosan-alginate polyelectric complexes are an effective drug delivery tool used in the pharmaceutical industry for controlled release of antimicrobials. These materials can be used for controlled release of nisin as a film or microparticle system. The goal of this research was to develop and characterize chitosan-alginate films and microparticles containing nisin, and understand the release kinetics of nisin in an aqueous system. The specific objectives were (1) to determine the factors affecting release of nisin from chitosan-alginate films, (2) to determine the release kinetics of nisin from chitosan-alginate films and study component interactions, and (3) to evaluate the release kinetics of nisin from chitosan-alginate microparticles. The effects of polymer concentration (0.5 and 1.0%), chitosan molecular weight (low and high) and ratio of chitosan to alginate (expressed as 33, 50 and 66% alginate fraction) were investigated on the release of nisin from chitosan-alginate films. The polymer concentration and alginate fraction were found to have a significant effect (p<0.05) on nisin release. Highest nisin release was obtained for films prepared using 0.5% polymer concentration and 33% alginate fraction. SEM images indicated that the ratio of chitosan to alginate influenced the film microstructure. The release kinetics of nisin from chitosan-alginate films prepared using various alginate fractions (33, 50 and 66%) was investigated using a two-temperature agar diffusion bioassay. The total amount of nisin released from films into an aqueous system decreased significantly (p<0.05) with an increase in alginate concentration. The mechanism of diffusion of nisin from all films was found to be Fickian, and the diffusion coefficients varied from 0.87 x 10-9 to 8.03 x 10-9 cm2/s. Strong complexation was confirmed between chitosan and alginate polymers within the films, particularly at high alginate fractions. Binding between nisin and alginate was also confirmed, resulting in reduction of freely available nisin within films. Chitosan-alginate microparticles containing nisin, were produced by preparing a calcium-alginate pre-gel, followed by chitosan complexation. Calcium concentrations ranging from 0.25 to 2 mM were found to be effective in inducing the formation of a coiled alginate nucleus. Particles obtained were in…

Subjects/Keywords: nisin; release; diffusion; chitosan-alginate; films; microparticles

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chandrasekar, V. (2015). Release kinetics of nisin from chitosan-alginate films and microparticles. (Doctoral Dissertation). Penn State University. Retrieved from https://etda.libraries.psu.edu/catalog/26302

Chicago Manual of Style (16^th Edition):

Chandrasekar, Vaishnavi. “Release kinetics of nisin from chitosan-alginate films and microparticles.” 2015. Doctoral Dissertation, Penn State University. Accessed August 22, 2019. https://etda.libraries.psu.edu/catalog/26302.

MLA Handbook (7^th Edition):

Chandrasekar, Vaishnavi. “Release kinetics of nisin from chitosan-alginate films and microparticles.” 2015. Web. 22 Aug 2019.

Vancouver:

Chandrasekar V. Release kinetics of nisin from chitosan-alginate films and microparticles. [Internet] [Doctoral dissertation]. Penn State University; 2015. [cited 2019 Aug 22]. Available from: https://etda.libraries.psu.edu/catalog/26302.

Council of Science Editors:

Chandrasekar V. Release kinetics of nisin from chitosan-alginate films and microparticles. [Doctoral Dissertation]. Penn State University; 2015. Available from: https://etda.libraries.psu.edu/catalog/26302

University of Manchester

17. Haener, Edgar Orell. Microfluidic Segregation of Capsules.

Degree: 2017, University of Manchester

URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:312097

► This thesis investigates the transport and sorting of capsules (elastic membranes enclosing a liquid core) using viscous flow in complex vessel geometries. Of particular interest… (more)

▼ This thesis investigates the transport and sorting of capsules (elastic membranes enclosing a liquid core) using viscous flow in complex vessel geometries. Of particular interest is passive sorting by deformability using only the fluid-structure interaction between the capsule, the viscous fluid and the geometry of the vessel. Millimetric alginate-ovalbumin capsules in the regime of negligible fluid inertia are used in this work. In order to characterise the elastic properties of the capsules, a novel numerical finite element model of the compression of a thick-shelled capsule between parallel plates is implemented. The constitutive model of the capsule membranes was determined by comparison to experimental data: a Yeoh constitutive model with the ratio of constants C₁ = 1, C₂ = 0 and C₃ = 10 describes the capsules used. Three geometries are investigated in this work. (i) A T-Junction bifurcation. Capsule deformation in the T-Junction bifurcation is characterised by the maximal length of the capsule L_max and depends on the ratio of viscous to elastic forces, the capillary number Ca. The maximal length, L_max, is especially sensitive at distinguishing soft capsules by their deformability. The sensitivity of L_max to capsule compliance and the large deformations that can be achieved makes the T-junction a promising geometry in which to measure elastic properties of the capsules. The rate of relaxation of the capsules after the bifurcation is independent of their deformation. (ii) A half-cylinder obstacle in a channel followed by a sudden expansion. We show that the half-cylinder obstacle causes capsule trajectories to vary depending on deformability. Capsules with a factor of three difference in deformability can be separated. A practical feature of the system is its relative insensitivity to the initial lateral position of the capsules in the channel. However, while the results are reproducible across different capsules, the variations in final position amount to 10 % at fixed parameters. As these experiments were conducted with the same capsule under identical flow conditions, this is likely to represent the best case scenario. (iii) We adapt the pinched flow fractionation (PFF) geometry to the sorting of capsules. We show that the standard PFF device cannot be used to sort capsules. However, a novel mode of operation, termed the ``T-Junction'' mode, shows great promise for the sorting of capsules. The PFF device in the T-Junction mode separates capsules with a factor of 1.5 difference in deformability. This is twice as sensitive as the half-cylinder device, although larger variability was observed in the PFF device. Advisors/Committee Members: HEIL, MATTHIAS M, Juel, Anne, Heil, Matthias.

Subjects/Keywords: Fluid Dynamics; Ovalbumin-alginate capsules; Passive sorting

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APA (6^th Edition):

Haener, E. O. (2017). Microfluidic Segregation of Capsules. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:312097

Chicago Manual of Style (16^th Edition):

Haener, Edgar Orell. “Microfluidic Segregation of Capsules.” 2017. Doctoral Dissertation, University of Manchester. Accessed August 22, 2019. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:312097.

MLA Handbook (7^th Edition):

Haener, Edgar Orell. “Microfluidic Segregation of Capsules.” 2017. Web. 22 Aug 2019.

Vancouver:

Haener EO. Microfluidic Segregation of Capsules. [Internet] [Doctoral dissertation]. University of Manchester; 2017. [cited 2019 Aug 22]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:312097.

Council of Science Editors:

Haener EO. Microfluidic Segregation of Capsules. [Doctoral Dissertation]. University of Manchester; 2017. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:312097

18. Dandoy, Philippe. Encapsulation de cellules animales au sein de billes inorganiques poreuses minéralisées : vers la création d'organes artificiels.

Degree: 2011, DIAL (Belgium)

URL: http://hdl.handle.net/2078.2/76437

►

The desire to find new treatments against non-infectious diseases like diabetes leads modern medicine to look towards emerging technologies. Current solutions to treat diabetes, such… (more)

▼

The desire to find new treatments against non-infectious diseases like diabetes leads modern medicine to look towards emerging technologies. Current solutions to treat diabetes, such as organ graft and insulin therapy, present major drawbacks, thus when considering the limited supply of live organs for transplantation, the development of artificial organs to replace deficient ones becomes a principle goal for clinicians. Cell transplants are an adequate solution and have already proved to be successful. However, this method only offers short time relief. This drives the need to develop a method where cells are protected against rejection without the continual use of immunosuppressive drugs. Considering the progress in biological entities immobilisation from bacteria, yeasts and plant cells, animal cell immobilisation shows great potential in the treatment of pathological disorders. For example a type 1 diabetic patient could be offered an implant comprised of live beta-cells immobilised within a porous, biocompatible and robust material, which could improve their day-to-day life. The aim of this project was to create a hybrid material which could act as a surrogate organ in place of the deficient one, offering new hope in the domain of cell therapy. In this thesis, research on the design of an artificial organ and the materials developed are described. With the aid of a suite of techniques, these matrices have been characterised to outline their properties and highlight their affinity with animal cells. Results show that cells were alive several weeks post-immobilisation. In vivo experiments using the most promising matrix were performed to study the integration of hybrid beads within a whole body, highlighting that very low to no inflammatory response in laboratory animals was caused by the subcutaneous implant. Final results suggest that an appropriate material, which was both robust and biocompatible, was successfully synthesised with a porous diameter fulfilling the requirements for cell immuno-isolation. Moreover, it did not provoke inflammation in the connective tissues surrounding the implant during the one-month in vivo study.

Le désir d’élaborer de nouveaux traitements face aux maladies non-infectieuses comme le diabète pousse la médecine moderne à se tourner vers les nouvelles technologies. La greffe d’organe étant aussi contraignante que l’insulinothérapie, la création d’organes artificiels pour suppléer un jour les organes déficients fait partie des rêves les plus convoités berçant l’imagination du praticien. Si la transplantation de cellules est une solution envisageable et déjà couronnée d’un certain succès, ce traitement n’a malheureusement que peu d’efficacité à long terme et impose de développer une méthode qui permettrait de protéger les nouvelles cellules du rejet sans recourir à la prise de médicaments immunosuppressifs. Devant les progrès obtenus pour l’immobilisation d’entités biologiques comme les bactéries, les levures ou les cellules végétales, celle de cellules animales est une nouvelle…

Advisors/Committee Members: FUNDP - SCHI_GCNM (groupe de chimie des nanomatériaux), FUNDP - -, Su, Bao-Lian, Michiels, Carine, Aprile, Carmela, Krief, Alain, Léonard, Alexandre, Meunier, Christophe, Wouters, Johan.

Subjects/Keywords: Cellules animales; Billes minéralisées; Biocompatibilité; Silice; Alginate

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APA (6^th Edition):

Dandoy, P. (2011). Encapsulation de cellules animales au sein de billes inorganiques poreuses minéralisées : vers la création d'organes artificiels. (Thesis). DIAL (Belgium). Retrieved from http://hdl.handle.net/2078.2/76437

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dandoy, Philippe. “Encapsulation de cellules animales au sein de billes inorganiques poreuses minéralisées : vers la création d'organes artificiels.” 2011. Thesis, DIAL (Belgium). Accessed August 22, 2019. http://hdl.handle.net/2078.2/76437.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dandoy, Philippe. “Encapsulation de cellules animales au sein de billes inorganiques poreuses minéralisées : vers la création d'organes artificiels.” 2011. Web. 22 Aug 2019.

Vancouver:

Dandoy P. Encapsulation de cellules animales au sein de billes inorganiques poreuses minéralisées : vers la création d'organes artificiels. [Internet] [Thesis]. DIAL (Belgium); 2011. [cited 2019 Aug 22]. Available from: http://hdl.handle.net/2078.2/76437.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dandoy P. Encapsulation de cellules animales au sein de billes inorganiques poreuses minéralisées : vers la création d'organes artificiels. [Thesis]. DIAL (Belgium); 2011. Available from: http://hdl.handle.net/2078.2/76437

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Toledo Health Science Campus

19. Shin, Junkyu. Evaluation of Calcium Alginate Microparticles Prepared Using a Novel Nebulized Aerosol Mediated Interfacial Crosslinking Method.

Degree: MSP, Pharmaceutical Sciences (Industrial Pharmacy), 2016, University of Toledo Health Science Campus

URL: http://rave.ohiolink.edu/etdc/view?acc_num=mco1469041620

► Alginate is a very popular biopolymer used in microencapsulation. Applications of alginate based microparticles can be found in pharmaceuticals, food, cosmetics, and tissue engineering industries.… (more)

Subjects/Keywords: Pharmaceuticals; calcium alginate, microparticle, nebulization, ionotropic

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APA (6^th Edition):

Shin, J. (2016). Evaluation of Calcium Alginate Microparticles Prepared Using a Novel Nebulized Aerosol Mediated Interfacial Crosslinking Method. (Masters Thesis). University of Toledo Health Science Campus. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=mco1469041620

Chicago Manual of Style (16^th Edition):

Shin, Junkyu. “Evaluation of Calcium Alginate Microparticles Prepared Using a Novel Nebulized Aerosol Mediated Interfacial Crosslinking Method.” 2016. Masters Thesis, University of Toledo Health Science Campus. Accessed August 22, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=mco1469041620.

MLA Handbook (7^th Edition):

Shin, Junkyu. “Evaluation of Calcium Alginate Microparticles Prepared Using a Novel Nebulized Aerosol Mediated Interfacial Crosslinking Method.” 2016. Web. 22 Aug 2019.

Vancouver:

Shin J. Evaluation of Calcium Alginate Microparticles Prepared Using a Novel Nebulized Aerosol Mediated Interfacial Crosslinking Method. [Internet] [Masters thesis]. University of Toledo Health Science Campus; 2016. [cited 2019 Aug 22]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=mco1469041620.

Council of Science Editors:

Shin J. Evaluation of Calcium Alginate Microparticles Prepared Using a Novel Nebulized Aerosol Mediated Interfacial Crosslinking Method. [Masters Thesis]. University of Toledo Health Science Campus; 2016. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=mco1469041620

Virginia Tech

20. Pawar, Siddhesh Nitin. Chemical Modification of Alginates in Organic Media.

Degree: PhD, Learning Sciences and Technologies, 2013, Virginia Tech

URL: http://hdl.handle.net/10919/52635

► Alginates are (1and4) linked linear copolysaccharides composed of B-D-mannuronic acid (M) and its C-5 epimer, a-L-guluronic acid (G). Several strategies to synthesize organically modified alginate… (more)

Subjects/Keywords: Alginate; derivatization; regioselective; acetate; modification; DS; gelling

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APA (6^th Edition):

Pawar, S. N. (2013). Chemical Modification of Alginates in Organic Media. (Doctoral Dissertation). Virginia Tech. Retrieved from http://hdl.handle.net/10919/52635

Chicago Manual of Style (16^th Edition):

Pawar, Siddhesh Nitin. “Chemical Modification of Alginates in Organic Media.” 2013. Doctoral Dissertation, Virginia Tech. Accessed August 22, 2019. http://hdl.handle.net/10919/52635.

MLA Handbook (7^th Edition):

Pawar, Siddhesh Nitin. “Chemical Modification of Alginates in Organic Media.” 2013. Web. 22 Aug 2019.

Vancouver:

Pawar SN. Chemical Modification of Alginates in Organic Media. [Internet] [Doctoral dissertation]. Virginia Tech; 2013. [cited 2019 Aug 22]. Available from: http://hdl.handle.net/10919/52635.

Council of Science Editors:

Pawar SN. Chemical Modification of Alginates in Organic Media. [Doctoral Dissertation]. Virginia Tech; 2013. Available from: http://hdl.handle.net/10919/52635

Univerzitet u Beogradu

21. Malagurski, Ivana S., 1982-. Dobijanje i karakterizacija nemineralizovanih i mineralizovanih biomaterijala na bazi polisaharida morskih algi i esencijalnog metala cinka.

Degree: Tehnološko-metalurški fakultet, 2018, Univerzitet u Beogradu

URL: https://fedorabg.bg.ac.rs/fedora/get/o:17314/bdef:Content/get

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Tehnološko inženjerstvo - Biohemijsko inženjerstvo i biotehnologija / Technological engineering - Biochemical engineering and biotechnology

U ovoj tezi je ispitana mogućnost sinteze novih, poboljšanih biomaterijala… (more)

Subjects/Keywords: alginate; agar; zinc; nanocomposites; biomineralization; antimicrobial activity

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APA (6^th Edition):

Malagurski, Ivana S., 1. (2018). Dobijanje i karakterizacija nemineralizovanih i mineralizovanih biomaterijala na bazi polisaharida morskih algi i esencijalnog metala cinka. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:17314/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Malagurski, Ivana S., 1982-. “Dobijanje i karakterizacija nemineralizovanih i mineralizovanih biomaterijala na bazi polisaharida morskih algi i esencijalnog metala cinka.” 2018. Thesis, Univerzitet u Beogradu. Accessed August 22, 2019. https://fedorabg.bg.ac.rs/fedora/get/o:17314/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Malagurski, Ivana S., 1982-. “Dobijanje i karakterizacija nemineralizovanih i mineralizovanih biomaterijala na bazi polisaharida morskih algi i esencijalnog metala cinka.” 2018. Web. 22 Aug 2019.

Vancouver:

Malagurski, Ivana S. 1. Dobijanje i karakterizacija nemineralizovanih i mineralizovanih biomaterijala na bazi polisaharida morskih algi i esencijalnog metala cinka. [Internet] [Thesis]. Univerzitet u Beogradu; 2018. [cited 2019 Aug 22]. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:17314/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Malagurski, Ivana S. 1. Dobijanje i karakterizacija nemineralizovanih i mineralizovanih biomaterijala na bazi polisaharida morskih algi i esencijalnog metala cinka. [Thesis]. Univerzitet u Beogradu; 2018. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:17314/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Ottawa

22. Parsian, Ava. Simvastatin Encapsulation in Alginate-Based Microspheres .

Degree: 2016, University of Ottawa

URL: http://hdl.handle.net/10393/34439

► Despite the great success of hip implant surgeries, wear particle-induced implant aseptic loosening still limits the implant longevity. Simvastatin, an FDA-approved cholesterol lowering statin, is… (more)

▼ Despite the great success of hip implant surgeries, wear particle-induced implant aseptic loosening still limits the implant longevity. Simvastatin, an FDA-approved cholesterol lowering statin, is a promising drug candidate for the treatment of implant aseptic loosening due to its anti-inflammatory properties as well as its ability to stimulate bone growth and inhibit bone resorption. In addition, alginate microspheres have been used extensively in drug delivery applications because of alginate properties, including biocompatibility and gelation in mild conditions. However, the hydrophobicity of simvastatin, as well as the large alginate microsphere pore size leading to the leakage of low molecular weight drugs are limiting factors for their use as a delivery system for simvastatin. Therefore, the objectives of this thesis were twofold: 1. To complex simvastatin with 2-hydroxypropyl-β-cyclodextrin (HP-βCD) in order to increase its solubility; and 2. To increase simvastatin encapsulation efficiency in alginate microspheres by coating the microspheres with chitosan, adding dextran sulfate in the alginate solution, and optimizing the gelation conditions used for the synthesis of the microspheres (e.g., volume of gelation medium, curing time, and addition of simvastatin in the gelation medium). Results showed that simvastatin complexation with HP-βCD increased with HP-βCD to simvastatin molar ratio, to a maximum of 97.6% at the molar ratio of 10. Results also showed that chitosan coating of the alginate microspheres increased simvastatin encapsulation efficiency (up to 10.6%), which was further improved (up to 14.0%) when adding 2.0% (w/v) dextran sulfate to the alginate solution. This increase was likely due to electrostatic interactions between dextran sulfate and chitosan in addition to alginate, resulting in a denser coating. Finally, the addition of simvastatin in the gelation medium was shown to also increase simvastatin encapsulation (up to 22.4%), likely because of a decrease in the diffusion of simvastatin out of the microspheres. Overall, this work completed the initial steps for the development of an alginate-based drug delivery system for simvastatin with the long-term goal of providing a local delivery of simvastatin to modulate implant aseptic loosening.

Subjects/Keywords: Drug Delivery System; Alginate; Microspheres; Encapsulation; Simvastatin

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APA (6^th Edition):

Parsian, A. (2016). Simvastatin Encapsulation in Alginate-Based Microspheres . (Thesis). University of Ottawa. Retrieved from http://hdl.handle.net/10393/34439

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Parsian, Ava. “Simvastatin Encapsulation in Alginate-Based Microspheres .” 2016. Thesis, University of Ottawa. Accessed August 22, 2019. http://hdl.handle.net/10393/34439.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Parsian, Ava. “Simvastatin Encapsulation in Alginate-Based Microspheres .” 2016. Web. 22 Aug 2019.

Vancouver:

Parsian A. Simvastatin Encapsulation in Alginate-Based Microspheres . [Internet] [Thesis]. University of Ottawa; 2016. [cited 2019 Aug 22]. Available from: http://hdl.handle.net/10393/34439.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Parsian A. Simvastatin Encapsulation in Alginate-Based Microspheres . [Thesis]. University of Ottawa; 2016. Available from: http://hdl.handle.net/10393/34439

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Toronto

23. Wong, Steven. Structure and Role of P. aeruginosa AlgL in Alginate Biosynthesis.

Degree: 2016, University of Toronto

URL: http://hdl.handle.net/1807/92619

►

During chronic infection, Pseudomonas aeruginosa generate alginate-containing biofilms in the lungs of cystic fibrosis patients. Mature bacterial alginate is a heteropolymer of Îą-L-guluronic acid and… (more)

Subjects/Keywords: Alginate; Bacteria; Biofilm; Infection; Lyase; Polysaccharide; 0487

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APA (6^th Edition):

Wong, S. (2016). Structure and Role of P. aeruginosa AlgL in Alginate Biosynthesis. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/92619

Chicago Manual of Style (16^th Edition):

Wong, Steven. “Structure and Role of P. aeruginosa AlgL in Alginate Biosynthesis.” 2016. Masters Thesis, University of Toronto. Accessed August 22, 2019. http://hdl.handle.net/1807/92619.

MLA Handbook (7^th Edition):

Wong, Steven. “Structure and Role of P. aeruginosa AlgL in Alginate Biosynthesis.” 2016. Web. 22 Aug 2019.

Vancouver:

Wong S. Structure and Role of P. aeruginosa AlgL in Alginate Biosynthesis. [Internet] [Masters thesis]. University of Toronto; 2016. [cited 2019 Aug 22]. Available from: http://hdl.handle.net/1807/92619.

Council of Science Editors:

Wong S. Structure and Role of P. aeruginosa AlgL in Alginate Biosynthesis. [Masters Thesis]. University of Toronto; 2016. Available from: http://hdl.handle.net/1807/92619

University of New South Wales

24. Lu, Wei. Alginate and cucurbit[n]uril as combined drug delivery system for albendazole: Experiments and mathematical modelling.

Degree: Physical, 2013, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/53119 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11805/SOURCE01?view=true

► A combined hydrogel-based drug delivery system consisting of alginate and cucurbit[n]uril (Q[n]) was prepared for the delivery of albendazole (ABZ), a sparingly soluble anthelmintic recently… (more)

▼ A combined hydrogel-based drug delivery system consisting of alginate and cucurbit[n]uril (Q[n]) was prepared for the delivery of albendazole (ABZ), a sparingly soluble anthelmintic recently found to have anti-cancer activity. Q[n] is a family of macrocyclic host molecules that has two roles in the delivery system described within this thesis. The smaller homologue, Q[5], served as an ionic cross-linker with two sodium ions bonded at both of its portals. The larger homologues, Q[7] and Q[8], encapsulated ABZ in their cavities. The encapsulation significantly increased the solubility of ABZ in water and achieved considerable drug loading content within the hydrogel, with a relatively high loading efficiency around 40%.Precursor solutions were prepared by dissolving sodium alginate in saturated Q[5] solutions. Different amounts of the drug complexes were dissolved to give precursor solutions of different formulation. The hydrogel beads were best form by dripping the precursor solutions into a pH1 gelling solution. The beads exhibited the same pH responsive swelling behaviour as calcium alginate hydrogel.Drug release studies were conducted in release media of low and high NaCl concentrations at three pH conditions of 6.3, 3 and 1. Percentage of the loaded drug released and drug release rates were found to be highly sensitive to the concentration of NaCl and pH but were also affected by other factors such as the type (size of the homologue used) and the amount of the drug complexes loaded into the gel. Complete release of the drug could be achieved only in the presence of ions, suggesting substantial electrostatic attraction between the drug complexes, the cross-linking Q[5] and the alginate.Experimental drug release data were analysed by mathematical models, which suggested that drug release was primarily controlled by diffusion, with a swelling controlled component, where ions were present. The fitted effective diffusion coefficient of the drug complexes ranged between ~3×10-8 and 7×10-7 cm2/s, depending on the type and the amount of the drug complexes loaded and the nature of the release media.It is proposed, based on the experimental results and the mathematical modelling, that both of the drug complexes formed aggregates with Q[5] through intermolecular interactions, which is a process initiated by electrostatic attraction. However, [email protected][8] had stronger interactions and formed larger aggregates than [email protected][7], and hence had a more retarded release.Slow release of ABZ over a few days was achieved in physiological saline with [email protected][8] and [email protected][7] offered faster release maintaining higher concentrations. Advisors/Committee Members: Day, Anthony, Physical, Environmental & Mathematical Sciences, UNSW Canberra, UNSW, Woodward, Cliff, Physical, Environmental & Mathematical Sciences, UNSW Canberra, UNSW.

Subjects/Keywords: Cucurbituril; Hygrogel; Drug delivery; Albendazole; Alginate

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APA (6^th Edition):

Lu, W. (2013). Alginate and cucurbit[n]uril as combined drug delivery system for albendazole: Experiments and mathematical modelling. (Masters Thesis). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/53119 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11805/SOURCE01?view=true

Chicago Manual of Style (16^th Edition):

Lu, Wei. “Alginate and cucurbit[n]uril as combined drug delivery system for albendazole: Experiments and mathematical modelling.” 2013. Masters Thesis, University of New South Wales. Accessed August 22, 2019. http://handle.unsw.edu.au/1959.4/53119 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11805/SOURCE01?view=true.

MLA Handbook (7^th Edition):

Lu, Wei. “Alginate and cucurbit[n]uril as combined drug delivery system for albendazole: Experiments and mathematical modelling.” 2013. Web. 22 Aug 2019.

Vancouver:

Lu W. Alginate and cucurbit[n]uril as combined drug delivery system for albendazole: Experiments and mathematical modelling. [Internet] [Masters thesis]. University of New South Wales; 2013. [cited 2019 Aug 22]. Available from: http://handle.unsw.edu.au/1959.4/53119 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11805/SOURCE01?view=true.

Council of Science Editors:

Lu W. Alginate and cucurbit[n]uril as combined drug delivery system for albendazole: Experiments and mathematical modelling. [Masters Thesis]. University of New South Wales; 2013. Available from: http://handle.unsw.edu.au/1959.4/53119 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11805/SOURCE01?view=true

University of New South Wales

25. Xin, Yongjia. Effect of calcium and iron(III) on membrane fouling under conditions typical of submerged membrane bioreactor treatment of wastewaters.

Degree: Civil & Environmental Engineering, 2015, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/55075 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:36468/SOURCE02?view=true

► While the use of submerged membrane bioreactor (MBR) technology has risen dramatically during last decade, the most significant challenge still remaining is the reduction in… (more)

▼ While the use of submerged membrane bioreactor (MBR) technology has risen dramatically during last decade, the most significant challenge still remaining is the reduction in the severity of membrane fouling. In addition, this filtration technology performs poorly in removal of dissolved contaminants such as phosphorus with addition of adsorbing chemicals such as iron necessary to ensure satisfactory effluent quality. The addition of chemicals such as iron salts, which readily hydrolyse and precipitate as iron oxyhydroxides on addition to the wastewater stream, may however exacerbate the fouling problem, particularly in submerged MBRs where a sedimentation step is not used. In such situations, optimizing the dosage of iron salts is critical in ensuring the most cost effective performance of the MBR however, the relationship between iron dosage and membrane fouling is not completely understood. In particular, the presence of other wastewater constituents such as monovalent and divalent ions (such as sodium and calcium) may significantly influence the interaction of iron with soluble microbial products (SMP) present in the MBR supernatant. In this thesis, the model polysaccharide alginate is used to investigate the interplay between SMP and iron and calcium under conditions typical of a submerged membrane bioreactor. The concentration of calcium present is shown to be a critical determinant of the severity of membrane fouling with low concentrations inducing alginate gelation and resultant severe membrane fouling while higher calcium concentrations result in gel breakage and alginate aggregation resulting in formation of porous cakes which facilitate rapid filtration. Our results also demonstrate that the presence of sodium may lead to a worsening of fouling as these ions block binding sites and limit the ability of calcium to induce aggregation. Comparison of the properties of the alginate assemblages formed in the presence of iron indicate that the Fe-alginate deposits induce even more severe fouling than Ca-alginate gels with lower concentrations of iron than calcium required to induce gelation. Increasing the concentration of iron leads eventually to a reduction in fouling propensity, most likely as a result of the adsorption of alginate to oxyhydroxide surfaces rather than alginate bridging as was the case for calcium. Importantly, the presence of calcium in a system to which iron salts are dosed is shown to lead to a significant reduction in fouling propensity. Investigations with SMP from an actual wastewater plant reveal similar interplay with iron and calcium as observed in the alginate system and highlight the possibility of fouling control through careful manipulation of iron and calcium concentrations in the supernatant. Advisors/Committee Members: Waite, T. David, Civil & Environmental Engineering, Faculty of Engineering, UNSW.

Subjects/Keywords: Calcium; Membrane fouling; Alginate; Iron (III)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Xin, Y. (2015). Effect of calcium and iron(III) on membrane fouling under conditions typical of submerged membrane bioreactor treatment of wastewaters. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/55075 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:36468/SOURCE02?view=true

Chicago Manual of Style (16^th Edition):

Xin, Yongjia. “Effect of calcium and iron(III) on membrane fouling under conditions typical of submerged membrane bioreactor treatment of wastewaters.” 2015. Doctoral Dissertation, University of New South Wales. Accessed August 22, 2019. http://handle.unsw.edu.au/1959.4/55075 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:36468/SOURCE02?view=true.

MLA Handbook (7^th Edition):

Xin, Yongjia. “Effect of calcium and iron(III) on membrane fouling under conditions typical of submerged membrane bioreactor treatment of wastewaters.” 2015. Web. 22 Aug 2019.

Vancouver:

Xin Y. Effect of calcium and iron(III) on membrane fouling under conditions typical of submerged membrane bioreactor treatment of wastewaters. [Internet] [Doctoral dissertation]. University of New South Wales; 2015. [cited 2019 Aug 22]. Available from: http://handle.unsw.edu.au/1959.4/55075 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:36468/SOURCE02?view=true.

Council of Science Editors:

Xin Y. Effect of calcium and iron(III) on membrane fouling under conditions typical of submerged membrane bioreactor treatment of wastewaters. [Doctoral Dissertation]. University of New South Wales; 2015. Available from: http://handle.unsw.edu.au/1959.4/55075 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:36468/SOURCE02?view=true

Queens University

26. Kapishon, Vitaliy. Modification of Alginate and Development of Polymeric Nanocarriers by Living Radical Polymerization for Controlled/Targeted Drug Delivery .

Degree: Chemical Engineering, 2015, Queens University

URL: http://hdl.handle.net/1974/13856

► Alginate-based amphiphilic graft copolymers were synthesized by single electron transfer living radical polymerization (SET-LRP), forming stable micelles during polymerization induced self-assembly (PISA). First, alginate macroinitiator… (more)

▼ Alginate-based amphiphilic graft copolymers were synthesized by single electron transfer living radical polymerization (SET-LRP), forming stable micelles during polymerization induced self-assembly (PISA). First, alginate macroinitiator was prepared by partial depolymerization of native alginate, solubility modification and attachment of initiator. Then, methyl methacrylate was polymerized from the alginate macroinitiator in mixtures of water and methanol forming poly(methyl methacrylate) grafts, prior to self-assembly. PISA of the amphiphilic graft-copolymer resulted in the formation of micelles with diameters of 50-300 nm. As the first reported case of LRP from alginate, this work introduces a synthetic route to of alginate-based hybrid polymers with a precise macromolecular architecture and desired functionalities. LRP from alginate can be applied to improve alginate-based hydrogel systems such as nano- and micro-hydrogel particles, islet encapsulation materials, hydrogel implants and topical applications. Such modified alginates can also improve the function and application of native alginates in food and agricultural technologies. Targeted drug delivery using polymeric nanostructures has been at the forefront of cancer research engineered for safer, more efficient and effective use of chemotherapy. Here, a new polymeric micelle delivery system was designed for active tumor targeting via receptor-induced endocytosis. Oseltamivir phosphate targets and inhibits Neu1 sialidase activity associated with receptor tyrosine kinases such as epidermal growth factor (EGF) receptors which are overexpressed on cancer cells. By decorating micelles with oseltamivir, it was found that micelles actively targeted human pancreatic PANC1 cancer cells. Amphiphilic block copolymers with oseltamivir conjugated at the hydrophilic end, oseltamivir-poly(polyethylene glycol methyl ether methacrylate)-block-poly(methyl methacrylate), were synthesized using reversible addition-fragmentation chain transfer (RAFT) living radical polymerization. Oseltamivir-conjugated micelles have self-assembling properties forming worm-like micellar structures. Oseltamivir-conjugated micelles dose dependently reduced the cell viability of PANC1 cells compared to micelles without oseltamivir. Oseltamivir-micelles loaded with hydrophobic dye produced internalization by PANC1. These results clearly indicate that oseltamivir-conjugated micelles show potential as an entirely new active tumor targeting drug delivery system with the ability to internalize hydrophobic chemotherapeutics by receptor-mediated endocytosis.

Subjects/Keywords: Living Radical Polymerization; Drug Delivery; Alginate; Biomaterials

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kapishon, V. (2015). Modification of Alginate and Development of Polymeric Nanocarriers by Living Radical Polymerization for Controlled/Targeted Drug Delivery . (Thesis). Queens University. Retrieved from http://hdl.handle.net/1974/13856

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kapishon, Vitaliy. “Modification of Alginate and Development of Polymeric Nanocarriers by Living Radical Polymerization for Controlled/Targeted Drug Delivery .” 2015. Thesis, Queens University. Accessed August 22, 2019. http://hdl.handle.net/1974/13856.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kapishon, Vitaliy. “Modification of Alginate and Development of Polymeric Nanocarriers by Living Radical Polymerization for Controlled/Targeted Drug Delivery .” 2015. Web. 22 Aug 2019.

Vancouver:

Kapishon V. Modification of Alginate and Development of Polymeric Nanocarriers by Living Radical Polymerization for Controlled/Targeted Drug Delivery . [Internet] [Thesis]. Queens University; 2015. [cited 2019 Aug 22]. Available from: http://hdl.handle.net/1974/13856.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kapishon V. Modification of Alginate and Development of Polymeric Nanocarriers by Living Radical Polymerization for Controlled/Targeted Drug Delivery . [Thesis]. Queens University; 2015. Available from: http://hdl.handle.net/1974/13856

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

27. Chaubaroux, Christophe. Revêtements à base de collagène pour la fonctionnalisation de biomatériaux : Collagen-based coatings for biomaterials functionalization.

Degree: Docteur es, Chimie physique, 2013, Université de Strasbourg

URL: http://www.theses.fr/2013STRAE004

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La fonctionnalisation des biomatériaux est une stratégie probante et prometteuse développée pour favoriser l’intégration de biomatériaux dans un organisme vivant. Le dépôt de films multicouches… (more)

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La fonctionnalisation des biomatériaux est une stratégie probante et prometteuse développée pour favoriser l’intégration de biomatériaux dans un organisme vivant. Le dépôt de films multicouches de polyélectrolytes est une méthode de fonctionnalisation de surfaces particulièrement adaptée au recouvrement d’implants. Ces surfaces modifiées pourront ainsi interagir avec leur environnement biologique. Au cours de ce travail de thèse, nous avons développé un nouveau revêtement, à base de composés naturels, capable de recouvrir plusieurs types de surfaces. De plus, ces revêtements originaux peuvent être utilisés pour orienter certains phénomènes cellulaires. Dans la première partie de ce travail de thèse, nous avons mis au point un nouveau système de films multicouches à base de collagène et d’alginate. La réticulation chimique avec la génipine, un agent naturel d’origine végétale (Gardenia Jasminoide), stabilise ces constructions pour une utilisation en conditions physiologiques. Les études d’adhérence et de prolifération de cellules endothéliales humaines ont montré que ces revêtements à base de constituants naturels sont des supports adéquats en vue d’applications biomédicales. Nous avons ensuite déposé des films collagène/alginate sur des implants en titane précédemment recouvert d’un gel microporeux en poly(acide lactique). Nous avons pu montrer que les films collagène/alginate favorisent la prolifération de cellules épithéliales, ce qui permettrait une meilleure intégration des implants. Dans la deuxième partie de notre travail, nous avons développé une technique permettant d’obtenir des revêtements et des membranes à base de films multicouches collagène/alginate ayant des structures fibrillaires orientées. L’alignement fibrillaire s’obtient par simple étirement des substrats élastiques en poly(diméthyl siloxane) (PDMS) sur lesquels sont déposés les revêtements collagène/alginate. La déformation longitudinale du substrat induit un alignement préférentiel des fibrilles de collagène du revêtement. L’étude de l’influence du taux d’étirement sur l’alignement des fibres a montré qu’il était possible de moduler cet alignement. Enfin, nous avons observé que le comportement de différents types cellulaires (fibroblastes et astrocytes) est modifié par l’alignement fibrillaire. On note que les cellules s’alignent dans la même direction que les fibrilles de collagène. A l’évidence, l’organisation fibrillaire du revêtement conditionne la géométrie de l’étalement cellulaire. Les cellules s’allongent lorsque les fibrilles sont alignées. De plus, il apparaît que la direction des divisions cellulaires est guidée par la direction de l’alignement des fibrilles de collagène dans le revêtement étiré. Cela signifie que les cellules sont guidées par les fibrilles de collagène alignées.

Biomaterial functionalization is a promising strategy developed to favor material implantation in a living organism. The deposition of polyelectrolyte multilayer films is a useful functionalization technique to coat implants. These modified surfaces may…

Advisors/Committee Members: Hemmerlé, Joseph (thesis director), Haïkel, Youssef (thesis director).

Subjects/Keywords: Collagène; Alginate; Multicouches; Alignement; Fonctionnalisation; Collagen; Alginate; Multilayer; Alignment; Functionalization; 541.33; 571.4

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chaubaroux, C. (2013). Revêtements à base de collagène pour la fonctionnalisation de biomatériaux : Collagen-based coatings for biomaterials functionalization. (Doctoral Dissertation). Université de Strasbourg. Retrieved from http://www.theses.fr/2013STRAE004

Chicago Manual of Style (16^th Edition):

Chaubaroux, Christophe. “Revêtements à base de collagène pour la fonctionnalisation de biomatériaux : Collagen-based coatings for biomaterials functionalization.” 2013. Doctoral Dissertation, Université de Strasbourg. Accessed August 22, 2019. http://www.theses.fr/2013STRAE004.

MLA Handbook (7^th Edition):

Chaubaroux, Christophe. “Revêtements à base de collagène pour la fonctionnalisation de biomatériaux : Collagen-based coatings for biomaterials functionalization.” 2013. Web. 22 Aug 2019.

Vancouver:

Chaubaroux C. Revêtements à base de collagène pour la fonctionnalisation de biomatériaux : Collagen-based coatings for biomaterials functionalization. [Internet] [Doctoral dissertation]. Université de Strasbourg; 2013. [cited 2019 Aug 22]. Available from: http://www.theses.fr/2013STRAE004.

Council of Science Editors:

Chaubaroux C. Revêtements à base de collagène pour la fonctionnalisation de biomatériaux : Collagen-based coatings for biomaterials functionalization. [Doctoral Dissertation]. Université de Strasbourg; 2013. Available from: http://www.theses.fr/2013STRAE004

Université de Grenoble

28. Dalle, Prisca. Système intégré pour l'encapsulation monocouche de cellules : Integrated system for simple layer cell encapsulation.

Degree: Docteur es, Biotechnologie, instrumentation, signal et imagerie pour la biologie, la médecine, et l’environnement, 2012, Université de Grenoble

URL: http://www.theses.fr/2012GRENS036

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L'implantation d'îlots de Langerhans microencapsulés est une thérapie prometteuse pour le diabète de type 1. Le concept est de rétablir la fonction de sécrétion d'insuline… (more)

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L'implantation d'îlots de Langerhans microencapsulés est une thérapie prometteuse pour le diabète de type 1. Le concept est de rétablir la fonction de sécrétion d'insuline tout en évitant le rejet de greffe. Les premiers essais cliniques ont reporté des résultats encourageants mais encore peu reproductibles. En effet, les techniques actuelles de production de microcapsules sont manuelles et au sein d'un même lot les capsules ne sont pas homogènes. L'utilisation de la microfluidique offre la possibilité de remédier à ce problème. Ce projet présente les différentes étapes d'optimisation d'un système microfluidique complexe qui automatise le procédé d'encapsulation monocouche de cellules dans des capsules polymériques d'alginate. Ce système comporte trois fonctions principales en série : la formation de gouttes monodisperses d'alginate en flux d'huile par un module MFFD (Micro Flow Focusing Device), le transfert de ces gouttes de la phase huileuse vers une phase aqueuse gélifiante de calcium et enfin un second transfert vers un flux à concentration physiologique. Des capsules monodisperses et sphériques ont été obtenues en sortie de ce système et des premiers tests d'encapsulation de cellules ont été réalisés. Les capsules produites par le système automatisé ont conduit à une première implantation chez le rongeur. Ce système est une première étape clé vers un dispositif instrumenté qui permettrait aux cliniciens d'encapsuler des îlots rapidement et de façon reproductible directement après leur isolement, puis de les implanter chez les patients diabétiques. Mots clés : encapsulation, îlots de Langerhans, alginate, gélification, microfluidique, automatisation, MFFD, transfert de phase.

Epileptic seizures arise from pathological synchronization of neuronal ensemble.Seizures originating from primary motor cortex are often pharmacoresistant, and many times unsuitable for respective surgery because of location of epileptic focus in eloquent area. Basal ganglia play important role in seizure propagation. Micro electrode recordings performed during previous studies indicated that input structures of basal ganglia such as GPe, Putamen and Subthalamic nucleus (STN) are strongly modified during seizures. For example the mean firing rate of neurons of the STN and Putamen increased and the percentage of oscillatory neurons synchronized with the ictal EEG was higher during seizures as compared to interictal periods. Pilot studies in humans have shown the possible beneficial effect of chronic DBS applied to STN in treatment of pharmacoresistant motor seizures. Our study was aimed at studying the therapeutic effect of electrical stimulation of input structures of basal ganglia . We first developed a stable, predictable primate model of focal motor epilepsy by intracortical injection of penicillin and we documented it's pharmacoresistence. We then stereotactically implanted DBS electrodes in the STN and Putamen. The stimulator was embedded at the back of the animals. Subthreshold electrical stimulations at 130 Hz were applied to STN.…

Advisors/Committee Members: Benhamou, Pierre-Yves (thesis director), Rivera, Florence (thesis director).

Subjects/Keywords: Microfluidique; Encapsulation; Gélification; Alginate; Cellules; In vivo; Microfluidic; Encaspsulation; Gelation; Alginate; Cell; In vivo

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dalle, P. (2012). Système intégré pour l'encapsulation monocouche de cellules : Integrated system for simple layer cell encapsulation. (Doctoral Dissertation). Université de Grenoble. Retrieved from http://www.theses.fr/2012GRENS036

Chicago Manual of Style (16^th Edition):

Dalle, Prisca. “Système intégré pour l'encapsulation monocouche de cellules : Integrated system for simple layer cell encapsulation.” 2012. Doctoral Dissertation, Université de Grenoble. Accessed August 22, 2019. http://www.theses.fr/2012GRENS036.

MLA Handbook (7^th Edition):

Dalle, Prisca. “Système intégré pour l'encapsulation monocouche de cellules : Integrated system for simple layer cell encapsulation.” 2012. Web. 22 Aug 2019.

Vancouver:

Dalle P. Système intégré pour l'encapsulation monocouche de cellules : Integrated system for simple layer cell encapsulation. [Internet] [Doctoral dissertation]. Université de Grenoble; 2012. [cited 2019 Aug 22]. Available from: http://www.theses.fr/2012GRENS036.

Council of Science Editors:

Dalle P. Système intégré pour l'encapsulation monocouche de cellules : Integrated system for simple layer cell encapsulation. [Doctoral Dissertation]. Université de Grenoble; 2012. Available from: http://www.theses.fr/2012GRENS036

29. Conzatti, Guillaume. Biomatériaux pour application chirurgicale : élaboration et fonctionnalisation pour une bioadhésion thermorégulée : Biomaterials for surgical application : elaboration and functionnalisation for a thermoregulated bioadhesion.

Degree: Docteur es, Biotechnologies, cancérologie, 2017, Université Toulouse III – Paul Sabatier

URL: http://www.theses.fr/2017TOU30159

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Avec une mortalité après pancréatectomie de l'ordre de 5 % et une morbidité aux alentours de 50 %, la chirurgie pancréatique est l'une des plus… (more)

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Avec une mortalité après pancréatectomie de l'ordre de 5 % et une morbidité aux alentours de 50 %, la chirurgie pancréatique est l'une des plus délicates des interventions digestives. L'une des complications les plus graves est l'apparition de fistules pancréatiques (FP), c'est-à-dire un défaut d'imperméabilisation au niveau des sutures, impliquant une fuite enzymatique dans le milieu péritonéal qui peut engager le pronostic vital du patient. A ce jour, aucun dispositif médical n'est indiqué dans la prévention de ces FPs. Ainsi, ce projet a pour ambition de concevoir et de valider un biomatériau constitué d'une matrice absorbante qui assurera la double fonction d'éponge et de réservoir d'agent antibactérien, sur laquelle un greffage chimique devrait conférer des propriétés bioadhésives thermorégulées. La première partie de ce travail est consacrée à l'optimisation de la matrice absorbante, à base d'alginate et de chitosane, déjà développée lors d'une précédente thèse. Trois types de procédés de séchages ont été comparés : le séchage par évaporation, la lyophilisation et le séchage en milieu CO2 supercritique. Ces différents procédés conduisent à des matériaux de structures internes et de porosités différentes. L'impact de ces différences de structure a été évalué en termes de capacité de gonflement dans différents milieux, dont un milieu pancréatique simulé, mais aussi en termes de résistance enzymatique et de libération de principe actif. En tenant compte des résultats obtenus, le séchage par évaporation a été identifié comme le plus approprié pour la suite de l'étude. Dans une deuxième partie, du poly(N-isopropylacrylamide) (PNIPAM) a été synthétisé par polymérisation contrôlée (RAFT) afin d'être greffé sur la matrice absorbante. Le PNIPAM est un polymère thermosensible dont les propriétés bioadhésives sont directement dépendantes de la température. Ce polymère est généralement bioadhésif au-dessus de sa " lower critical solution température " (LCST), située aux alentours de 32 °C. Dans cette étude la masse molaire et la densité de greffage du PNIPAM sont les deux principaux paramètres étudiés pour son greffage sur les matrices. Enfin, les propriétés de surface des matrices greffées ont été caractérisées. Les matériaux ont montré, in vitro, des propriétés bioadhésives thermosensibles, avec une bioadhésion cellulaire observée principalement au-delà de la LCST. Les essais ex vivo ont cependant montré une bioadhésion sur organe plus importante en dessous de celle-ci. Cette étude a permis de mettre au point des biomatériaux absorbants aux propriétés de surface thermorégulées. Une compréhension plus fine des relations propriétés de surface/propriétés d'usage permettrait d'optimiser les propriétés de bioadhésion thermorégulée.

Pancreatic surgery, which leads to 5 % of mortality and around 50 % of morbidity, is one of the most critical digestive operations. The most serious complication is the appearance of pancreatic fistulas (PFs), i.e. enzymatic leaks from the surgical sutures to the peritoneal environment that can…

Advisors/Committee Members: Carrère, Nicolas (thesis director), Tourrette, Audrey (thesis director).

Subjects/Keywords: Biomatériaux; Fonctionalisation de surface; Biopolymères; Alginate; Bioadhésion; Chitosane; Biomaterial; Surface functionalisation; Biopolymers; Alginate; Bioadhesion; Chitosan

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Conzatti, G. (2017). Biomatériaux pour application chirurgicale : élaboration et fonctionnalisation pour une bioadhésion thermorégulée : Biomaterials for surgical application : elaboration and functionnalisation for a thermoregulated bioadhesion. (Doctoral Dissertation). Université Toulouse III – Paul Sabatier. Retrieved from http://www.theses.fr/2017TOU30159

Chicago Manual of Style (16^th Edition):

Conzatti, Guillaume. “Biomatériaux pour application chirurgicale : élaboration et fonctionnalisation pour une bioadhésion thermorégulée : Biomaterials for surgical application : elaboration and functionnalisation for a thermoregulated bioadhesion.” 2017. Doctoral Dissertation, Université Toulouse III – Paul Sabatier. Accessed August 22, 2019. http://www.theses.fr/2017TOU30159.

MLA Handbook (7^th Edition):

Conzatti, Guillaume. “Biomatériaux pour application chirurgicale : élaboration et fonctionnalisation pour une bioadhésion thermorégulée : Biomaterials for surgical application : elaboration and functionnalisation for a thermoregulated bioadhesion.” 2017. Web. 22 Aug 2019.

Vancouver:

Conzatti G. Biomatériaux pour application chirurgicale : élaboration et fonctionnalisation pour une bioadhésion thermorégulée : Biomaterials for surgical application : elaboration and functionnalisation for a thermoregulated bioadhesion. [Internet] [Doctoral dissertation]. Université Toulouse III – Paul Sabatier; 2017. [cited 2019 Aug 22]. Available from: http://www.theses.fr/2017TOU30159.

Council of Science Editors:

Conzatti G. Biomatériaux pour application chirurgicale : élaboration et fonctionnalisation pour une bioadhésion thermorégulée : Biomaterials for surgical application : elaboration and functionnalisation for a thermoregulated bioadhesion. [Doctoral Dissertation]. Université Toulouse III – Paul Sabatier; 2017. Available from: http://www.theses.fr/2017TOU30159

University of Saskatchewan

30. Zhai, Peng. Alginate Microspheres for Protein Delivery in Tissue Engineering.

Degree: 2012, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2012-05-461

► Three-dimensional polymeric scaffolds have been widely used in tissue engineering for protein delivery. Scaffolds fabricated with different biomaterials and structures display various functions in protein… (more)

▼ Three-dimensional polymeric scaffolds have been widely used in tissue engineering for protein delivery. Scaffolds fabricated with different biomaterials and structures display various functions in protein delivery. A microsphere based delivery system is one sophisticated method. In this research, the potentials of alginate microspheres as protein carriers were tested. Alginate microspheres were prepared by a water-in-oil emulsion external gelation technique and loaded with bovine serum albumin (BSA) or DyLight 800 dye-labeled rabbit immunoglobulin G (IgG). Chitosan coated protein-loaded alginate microspheres were also prepared. The effects of process parameters on microsphere size, size distribution, encapsulation efficiency, and in vitro protein release profiles were investigated. Scanning electron microscopic photos showed that high dispersing force and high calcium chloride concentration produce small and uniform alginate microspheres with spherical shape and smooth surface. The release profiles indicated that BSA release from large and heterogeneous alginate microspheres was rapid and had a large initial burst release, and IgG release from small and homogeneous microspheres was slower and had lower initial burst release. Chitosan coating caused slower protein release compared to uncoated alginate microspheres in all cases. Protein-loaded microspheres were incorporated into alginate cylindrical scaffolds by long-term moulding in tubing for BSA or by fast gelation extruded from tubing for IgG. The scaffolds contained dried BSA loaded microspheres prepared using either protein incorporation or incubation methods, or with wet IgG-loaded microspheres with different concentrations. The release studies of BSA indicated that dried microspheres provided fast release possibly. The IgG release from scaffolds illustrated that chitosan coated IgG microspheres had more prolonged release profiles, and fast gelation of scaffolds could potentially eliminate protein loss during long-term gelation. All release profiles of scaffolds demonstrated that the initial burst effect was diminished and the release was extended by using a delivery system in which microspheres were incorporated into larger scaffolds. These prepared alginate microspheres and microsphere-incorporated scaffolds have been proven to have abilities of carrying and releasing proteins. Their applications toward delivery of functional proteins at the target site in patients for therapeutic purposes should be considered. Advisors/Committee Members: Chen, Daniel X. B., Schreyer, David J..

Subjects/Keywords: Alginate microspheres; Uniform size; Chitosan coating; Protein release; Alginate scaffolds; Tissue engineering

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APA (6^th Edition):

Zhai, P. (2012). Alginate Microspheres for Protein Delivery in Tissue Engineering. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2012-05-461

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zhai, Peng. “Alginate Microspheres for Protein Delivery in Tissue Engineering.” 2012. Thesis, University of Saskatchewan. Accessed August 22, 2019. http://hdl.handle.net/10388/ETD-2012-05-461.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zhai, Peng. “Alginate Microspheres for Protein Delivery in Tissue Engineering.” 2012. Web. 22 Aug 2019.

Vancouver:

Zhai P. Alginate Microspheres for Protein Delivery in Tissue Engineering. [Internet] [Thesis]. University of Saskatchewan; 2012. [cited 2019 Aug 22]. Available from: http://hdl.handle.net/10388/ETD-2012-05-461.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zhai P. Alginate Microspheres for Protein Delivery in Tissue Engineering. [Thesis]. University of Saskatchewan; 2012. Available from: http://hdl.handle.net/10388/ETD-2012-05-461

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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