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You searched for subject:(Alergia a fungos). Showing records 1 – 2 of 2 total matches.

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1. Santos, Bruno Fernandes dos. Purification and characterization of a serine protease from Alternaria alternata.

Degree: 2015, RCAAP

Alternaria alternata is a common environmental allergenic fungi. The prevalence of Alternaria allergies varies in different geographic regions. A multicenter study performed in a group of almost 900 patients with allergies found hypersensitivity to Alternaria in 3–20% of the individuals (3 % in Portugal to 20 % in Spain). To date, 17 allergens of A. alternata have been identified. Different studies demonstrated the existence of A. alternata serine proteases that can induce lung inflammation and airway epithelial cell activation via PAR2. In 2014, the Alt a 15 (recombinant A. alternata serine protease) was added to allergens list of the official Allergen nomenclature subcommittee. In the present project, we aimed to purify the native serine protease from the A. alternata extract since this form is very important for carrying out characterization studies and to provide a good foundation for improving the accuracy of the diagnosis and the understanding and management of IgE-mediated fungal diseases. The purification protocol involved a first step of affinity chromatography using a Concanavalin A Sepharose column and a second purification step of ion exchange chromatography using a DEAE-Sepharose column. A global 28.03-fold purification of serine protease was obtained with a specific activity of 144.33 U/mg. The molecular weight of the enzyme was determined as 40 kDa by using SDS-PAGE, and the optimum pH and temperature were 8.0 and 50º C, respectively. The kinetic parameters KM and Vmax, determined against substrate casein, were 0.51 mg/mL and 7.38 µmol/min/mL, respectively. The protease was strongly inhibited by PMSF (phenylmethyl sulfony fluorid) indicating it is a serine protease. The purification strategy applied in this work could be of great importance to open a new possibility for obtaining native A. alternata serine proteases for application in diagnosis or clinical purposes.

Nos últimos anos, tem vindo a aumentar a prevalência das doenças alérgicas em especial nos países industrializados. Estudos indicam que cerca de um terço da população ocidental sofre de uma ou mais formas de doenças alérgicas. De uma maneira simples, a doença alérgica pode ser definida como uma reação exacerbada do sistema imunitário contra determinados antigénios presentes no meio ambiente (alergénios), mas que na verdade não representam qualquer perigo para indivíduos não sensibilizados. Este tipo de doença está normalmente associada a uma produção aumentade de IgE específica para um determinado alergénio, sendo que a tendência pessoal ou familiar para a produção exacerbada deste tipo de imunoglobulina é denominada de atopia. De entre as fontes alergénicas ambientais capazes de induzir reações de hipersensibilidade, os fungos surgem como um dos principais responsáveis pelo desenvolvimento, persistência e severidade de doença alérgica respiratória, nomeadamente da asma e da rinite. A Alternaria alternata é uma das principais espécies fúngicas associadas a doenças alérgicas tendo, até a data, sido identificados 17 alergénios diferentes…

Advisors/Committee Members: Bicho, Diana Faria, Tomaz, Cândida Ascensão Teixeira.

Subjects/Keywords: Alergia A Fungos; Alternaria Alternata; Cromatografia; Purificação; Serina Proteases; Domínio/Área Científica::Ciências Médicas::Ciências da Saúde

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Santos, B. F. d. (2015). Purification and characterization of a serine protease from Alternaria alternata. (Thesis). RCAAP. Retrieved from https://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/5962

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Santos, Bruno Fernandes dos. “Purification and characterization of a serine protease from Alternaria alternata.” 2015. Thesis, RCAAP. Accessed July 16, 2019. https://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/5962.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Santos, Bruno Fernandes dos. “Purification and characterization of a serine protease from Alternaria alternata.” 2015. Web. 16 Jul 2019.

Vancouver:

Santos BFd. Purification and characterization of a serine protease from Alternaria alternata. [Internet] [Thesis]. RCAAP; 2015. [cited 2019 Jul 16]. Available from: https://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/5962.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Santos BFd. Purification and characterization of a serine protease from Alternaria alternata. [Thesis]. RCAAP; 2015. Available from: https://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/5962

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. Gabriel, Marta Sofia da Fonseca. Evaluation of Alt a 1 as specific marker of exposure to fungal allergenic sources and clinical relevance of a manganese-dependent superoxide dismutase and a serine protease as new Alternaria alternata allergens.

Degree: 2016, RCAAP

Allergic diseases are considered to be one of the epidemics of the century, affecting approximately one-third of the general population. Classically, among the allergenic sources able to induce IgE-mediated reactions, fungi have been one of the less favored areas of study. It has been demonstrated that sensitization to fungal aeroallergens, particularly from Alternaria alternata, represents an unequivocal risk factor for the development, persistence and severity of asthma. Thus, the better understanding and management of fungal allergy, namely by improvements in the actual assessment and diagnostic approaches are needed. Regarding the assessment of fungal exposure, actual methodologies are generally laborious and time-consuming making difficult to establish or exclude a fungal contamination and potential associations with allergic disease. In terms of diagnosis, the main difficulty arises from the high number of patients that are apparently sensitized to multiple fungi in which the identification of primary cause of sensitization is complex. Because A. alternata is one of the most abundant and potent source of airborne allergens, the panel of allergenic components produced by this fungal specie, seems to be a very relevant target of study. Among the several allergens described in this mold, Alt a 1 has been demonstrated to be the most important, sensitizing approximately 80% of A. alternata allergic patients. For this reason, Alt a 1 has been used as the diagnostic marker of genuine sensitization to A. alternata. However, given the complex A. alternata sensitization data, which shows a significant level of polysensitization, due to co-sensitization and/or cross-reactivity to several other phylogenetically related and non-related molds, other allergenic A. alternata components should be studied to explain the whole broad range of reported clinical observations. In the last years, componentresolved diagnosis (CRD) has been shown to be a valuable tool to elucidate clinical observations and to differentiate between cases of co-sensitization and cross-reactivity. Nevertheless, the actual available panel of A. alternata allergens appears to not be enough for achieving an accurate diagnosis and prognosis of sensitization to this mold. Considering the above mentioned facts, the major aims of this work were, on one hand to develop an approach to specifically detect Alternaria and related species by using the A. alternata major allergen, Alt a 1, as a specie-specific molecular marker. And, on other hand, to characterize two new cross-reactive A. alternata allergens belonging to the manganesedependent superoxide dismutase (MnSOD) and serine protease (SP) protein families and to study their role in A. alternata sensitization. The strategies used to accomplish both aims made use of phylogenetic relationships among fungal species that share A. alternata allergen homologues. First, investigating conservation of the genes encoding for Alt a 1 and its homologues which allowed the design of a set of primers in the conserved… Advisors/Committee Members: Tomaz, Cândida Ascensão Teixeira, Marcos, Jorge Martinez.

Subjects/Keywords: Alergénios; Alergia a fungos; Alternaria alternata; Anafilaxia; Asma; Aspergilose broncopulmonar alérgica; Exposição ambiental; Filogenética; Protease de serina; Proteínas homólogas; Reatividade cruzada; Recombinantes; Sensibilização múltipla; Superóxido dismutase dependente de manganês; Domínio/Área Científica::Ciências Naturais::Outras Ciências Naturais

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Gabriel, M. S. d. F. (2016). Evaluation of Alt a 1 as specific marker of exposure to fungal allergenic sources and clinical relevance of a manganese-dependent superoxide dismutase and a serine protease as new Alternaria alternata allergens. (Thesis). RCAAP. Retrieved from https://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/4195

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Gabriel, Marta Sofia da Fonseca. “Evaluation of Alt a 1 as specific marker of exposure to fungal allergenic sources and clinical relevance of a manganese-dependent superoxide dismutase and a serine protease as new Alternaria alternata allergens.” 2016. Thesis, RCAAP. Accessed July 16, 2019. https://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/4195.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Gabriel, Marta Sofia da Fonseca. “Evaluation of Alt a 1 as specific marker of exposure to fungal allergenic sources and clinical relevance of a manganese-dependent superoxide dismutase and a serine protease as new Alternaria alternata allergens.” 2016. Web. 16 Jul 2019.

Vancouver:

Gabriel MSdF. Evaluation of Alt a 1 as specific marker of exposure to fungal allergenic sources and clinical relevance of a manganese-dependent superoxide dismutase and a serine protease as new Alternaria alternata allergens. [Internet] [Thesis]. RCAAP; 2016. [cited 2019 Jul 16]. Available from: https://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/4195.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gabriel MSdF. Evaluation of Alt a 1 as specific marker of exposure to fungal allergenic sources and clinical relevance of a manganese-dependent superoxide dismutase and a serine protease as new Alternaria alternata allergens. [Thesis]. RCAAP; 2016. Available from: https://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/4195

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.