subject:(Affinity)

Clemson University

1. Trang, Hung Khiem. Development of Functionalized Capillary-Channeled Polymer (C-CP) Fibers as Stationary Phase for Affinity Chromatography.

Degree: PhD, Chemistry, 2019, Clemson University

URL: https://tigerprints.clemson.edu/all_dissertations/2532

► At the heart of the chromatography technique, the stationary phase is the essential component that dictates multiple aspects of the separation process. Diverse chemistry… (more)

▼ At the heart of the chromatography technique, the stationary phase is the essential component that dictates multiple aspects of the separation process. Diverse chemistry of the stationary phases, in combination with the choice of appropriate base support materials, allows chromatography to be used in a wide range of applications ranging from the high-performance separations of trace analytes in complex biological samples for chemical detection and quantification to the large-scale purification of a recombinant protein from a complex media. Traditional chromatographic stationary phases mostly utilize porous materials for the high surface area and versatility for chemical modification. However, in many cases, these materials suffer from high operating back-pressure and inefficient mass transfer. Joining the efforts to investigate alternative phases, Marcus research group (Clemson University) has investigated the potentials of capillary-channeled polymer (C-CP) fibers for the past 15 years. These fibers are characterized by eight capillary channels running down the length of the fiber, allowing the fibers to interdigitate to form well-aligned micrometer-sized channels when packed into a column format. It is the unique shape that allows separations to be conducted at high linear velocities (>75 mm s−1) with high column permeability (<0.14 MPa cm−1). The highly efficient fluid movement through the narrow channels composed of non-porous C-CP fibers gives rise to favorable mass transfer rates, facilitating fast protein separations and processing. Moreover, another advantage of C-CP fiber supports over other commercial sorbents is that these fibers are quite stable over a wide pH range and are fairly inexpensive (<$ 100 lb−1). Additionally, packing a C-CP fiber column is a simple process that can be conveniently performed without any special equipment. The concepts of using natural and synthetic polymer fibers as chromatographic stationary phases are not new. Potential advantages cited include low costs, ease in column fabrication, low operation backpressure, facile solute mass transfer, and a general ease of tailoring fiber surfaces to affect high levels of chemical specificity. There have been several surface modification techniques reported for polymer fibers in the literature. However, many of these modification methods can be detrimental to the physical properties of the polymer by destroying the polymer backbone. In order to further exploit the advantageous fluidic properties of C-CP fiber columns without compromising the fiber integrity, milder modification approaches have been a focus of this group recently, including covalent coupling and direct ligand adsorption methods. The research studies reported in this work focus on the development of functionalized C-CP fiber for a variety of affinity chromatography applications. The ultimate goal is to investigate non-invasive modification schemes that do not compromise the mechanical strength and fluidic properties of C-CP fibers. Affinity ligands are… Advisors/Committee Members: R. Kenneth Marcus, George Chumanov, Carlos D Garcia, Sarah W Harcum.

Subjects/Keywords: affinity; chromatography; fiber

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APA (6^th Edition):

Trang, H. K. (2019). Development of Functionalized Capillary-Channeled Polymer (C-CP) Fibers as Stationary Phase for Affinity Chromatography. (Doctoral Dissertation). Clemson University. Retrieved from https://tigerprints.clemson.edu/all_dissertations/2532

Chicago Manual of Style (16^th Edition):

Trang, Hung Khiem. “Development of Functionalized Capillary-Channeled Polymer (C-CP) Fibers as Stationary Phase for Affinity Chromatography.” 2019. Doctoral Dissertation, Clemson University. Accessed April 11, 2021. https://tigerprints.clemson.edu/all_dissertations/2532.

MLA Handbook (7^th Edition):

Trang, Hung Khiem. “Development of Functionalized Capillary-Channeled Polymer (C-CP) Fibers as Stationary Phase for Affinity Chromatography.” 2019. Web. 11 Apr 2021.

Vancouver:

Trang HK. Development of Functionalized Capillary-Channeled Polymer (C-CP) Fibers as Stationary Phase for Affinity Chromatography. [Internet] [Doctoral dissertation]. Clemson University; 2019. [cited 2021 Apr 11]. Available from: https://tigerprints.clemson.edu/all_dissertations/2532.

Council of Science Editors:

Trang HK. Development of Functionalized Capillary-Channeled Polymer (C-CP) Fibers as Stationary Phase for Affinity Chromatography. [Doctoral Dissertation]. Clemson University; 2019. Available from: https://tigerprints.clemson.edu/all_dissertations/2532

University of Illinois – Urbana-Champaign

2. Ahmed, Syeda Tajin. Investigation of minimum fragment requirement for mimicking n-cadherin mediated adhesion.

Degree: MS, Chemical Engineering, 2018, University of Illinois – Urbana-Champaign

URL: http://hdl.handle.net/2142/102855

► Cadherins are transmembrane glycoproteins that are involved in maintaining integrity of cell junctions, morphogenesis, cell sorting and many more important biological processes. In my thesis,… (more)

▼ Cadherins are transmembrane glycoproteins that are involved in maintaining integrity of cell junctions, morphogenesis, cell sorting and many more important biological processes. In my thesis, I have focused on studying the binding affinity of a class of cadherin superfamily called N-cadherins. Prior studies have demonstrated that N-cadherins can promote neuronal growth and axonal regeneration in vitro. Moreover, there is a growing interest in development of small molecules targeting N-cadherins and designing of therapeutic agents to promote cell survival and axonal regeneration as well as inhibit cadherin mediated signaling. For in vitro studies, knowing the minimum fragments of adhesive binding domain i.e. extracellular domains that can recapitulate the wild type N-cadherin binding functions can help develop designing of biomaterials and platforms to study N-cadherin interactions. Two dimensional affinity measurements reveal probability of stable bond formation during homophilic and/or heterophilic interactions between cadherins via extracellular domains. In order to determine the minimum fragment of the extracellular domain required for binding to mimic full length cadherin and wild type cadherins expressed on cell surfaces, Micropipette Aspiration Assay (MPA) was performed. Using N-cadherin as a model for classical cadherins, the binding affinity of full length N-cadherin (N-cad EC1-5) expressed on a test cell surface (here, mesenchymal stem cell (MSCs) with the first two extracellular domains (N-cad EC1-2) and the binding pocket sequence HAVDI peptide and full length N-cadherin (N-cad EC 1-5) were measured by this assay. The first chapter of my thesis, I talked about the structural differences of most studied cadherins E and N-cadherins and briefly described the importance of the flanking amino acid of binding pocket sequence HAVDI in N-cadherins. In the following chapter, I describe the experimental approach for determining the binding affinities of N-cadherin fragments and compared the 2D binding affinity results with that of the full length wild type N-cadherin expressed on MSCs. Surprisingly, the results of the study revealed that there is no significantly difference in binding affinity between N-cad EC1-2 and N-cad EC1-5 as well as HAVDI and N-cad EC1-5. The MPA studies were conducted with both wild type MSCs expressing N-cad EC1-5 as well as RBCs modified with N-cad EC1-5. However, there is a lower binding affinity between E-cadherin and HAVDI than that between N-cadherin and HAVDI. These results demonstrate the importance of the flanking amino acids next to HAV sequence in cadherin specificity as well as the possible application of small peptide sequence HAVDI as a novel antagonist in processes involving N-cadherin mediated adhesion. Advisors/Committee Members: Leckband, Deborah E. (advisor).

Subjects/Keywords: Cadherin; binding affinity

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APA (6^th Edition):

Ahmed, S. T. (2018). Investigation of minimum fragment requirement for mimicking n-cadherin mediated adhesion. (Thesis). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/102855

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ahmed, Syeda Tajin. “Investigation of minimum fragment requirement for mimicking n-cadherin mediated adhesion.” 2018. Thesis, University of Illinois – Urbana-Champaign. Accessed April 11, 2021. http://hdl.handle.net/2142/102855.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ahmed, Syeda Tajin. “Investigation of minimum fragment requirement for mimicking n-cadherin mediated adhesion.” 2018. Web. 11 Apr 2021.

Vancouver:

Ahmed ST. Investigation of minimum fragment requirement for mimicking n-cadherin mediated adhesion. [Internet] [Thesis]. University of Illinois – Urbana-Champaign; 2018. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/2142/102855.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ahmed ST. Investigation of minimum fragment requirement for mimicking n-cadherin mediated adhesion. [Thesis]. University of Illinois – Urbana-Champaign; 2018. Available from: http://hdl.handle.net/2142/102855

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Alberta

3. Diaz Satizabal, Laura P. Melano-macrophage characterization and their possible role in the goldfish (Carassius auratus) antibody affinity maturation.

Degree: MS, Department of Biological Sciences, 2013, University of Alberta

URL: https://era.library.ualberta.ca/files/0v838097f

► During the antibody affinity maturation process, changes to the immunoglobulin genes are initiated by the B-cell specific enzyme activation-induced cytidine deaminase (AID). These changes are… (more)

Subjects/Keywords: Melano-macrophage; Affinity maturation

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APA (6^th Edition):

Diaz Satizabal, L. P. (2013). Melano-macrophage characterization and their possible role in the goldfish (Carassius auratus) antibody affinity maturation. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/0v838097f

Chicago Manual of Style (16^th Edition):

Diaz Satizabal, Laura P. “Melano-macrophage characterization and their possible role in the goldfish (Carassius auratus) antibody affinity maturation.” 2013. Masters Thesis, University of Alberta. Accessed April 11, 2021. https://era.library.ualberta.ca/files/0v838097f.

MLA Handbook (7^th Edition):

Diaz Satizabal, Laura P. “Melano-macrophage characterization and their possible role in the goldfish (Carassius auratus) antibody affinity maturation.” 2013. Web. 11 Apr 2021.

Vancouver:

Diaz Satizabal LP. Melano-macrophage characterization and their possible role in the goldfish (Carassius auratus) antibody affinity maturation. [Internet] [Masters thesis]. University of Alberta; 2013. [cited 2021 Apr 11]. Available from: https://era.library.ualberta.ca/files/0v838097f.

Council of Science Editors:

Diaz Satizabal LP. Melano-macrophage characterization and their possible role in the goldfish (Carassius auratus) antibody affinity maturation. [Masters Thesis]. University of Alberta; 2013. Available from: https://era.library.ualberta.ca/files/0v838097f

University of Alberta

4. Zhang, Le. Functionalized Bead Based Microchip for Immunoassay and Virus Immuno-affinity Chromatography.

Degree: MS, Department of Chemistry, 2014, University of Alberta

URL: https://era.library.ualberta.ca/files/cth83kz515

► We demonstrate the functionalization of a highly ordered porous molecular sieving matrix created by colloidal self-assembly (CSA) of 2 micrometer diameter silica particles in microfluidic… (more)

▼ We demonstrate the functionalization of a highly ordered porous molecular sieving matrix created by colloidal self-assembly (CSA) of 2 micrometer diameter silica particles in microfluidic chips, for highly efficient immuno-capture of viruses. By tuning the particle size, with appropriate surface chemistry, we can easily match the pore size which could maximize biological species-particle wall collision, leading high capture efficiency. The ordered uniform lattice of pores, which are 15 % of the size of the particles used, should prove ideal for the capture of viruses (on the 50-150 nm scale in size). We report on characterization of capture beds with 300 nm pores. These beds were used to detect fluorescein using immobilized anti-fluorescein on the bed, and also to capture and detect type-5 adenovirus in suspension, and in infected cells using appropriate antibodies. We demonstrate the performance of the concept using a fully packed column for fluorescein immunoassay. We used 2 μm carboxylated silica particles self-assembled into a 6 mm long-bed, modified with EDC/NHS, and immobilized anti-fluorescein antibody or type-5 adenovirus-recognizing antibody. An electric field was applied to utilize electro-osmotic flow as the solvent pumping force. A 4.51 nM fluorescein solution was captured by immuno-affinity using anti-fluorescein antibody, and then released with an eluent to an empty downstream analysis region to give a very large signal, providing a positive control. Similar experiments were performed with type-5 adenovirus, demonstrating detection at a concentration of 8.3 × 103 viral particles (VP) per milliliter. Adenovirus in celllysate was also detected in this microchip, with the lowest concentration detectable at 1.5 × 103 PFU/mL. Combination of advanced biological detection methods with microfluidics based extraction and concentration techniques has tremendous potential for realization of a portable, cost effective and sensitive pathogen detection system. Taking advantage of the unique fluid flow characteristics of CSA structures on an even smaller scale than employed here, faster, more sensitive and more economical capture and pre-concentration techniques for diagnostic assays for a wider range of analytes can be developed.

Subjects/Keywords: Microfluidic; Immunoassay; Immuno-affinity Chromatography

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APA (6^th Edition):

Zhang, L. (2014). Functionalized Bead Based Microchip for Immunoassay and Virus Immuno-affinity Chromatography. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/cth83kz515

Chicago Manual of Style (16^th Edition):

Zhang, Le. “Functionalized Bead Based Microchip for Immunoassay and Virus Immuno-affinity Chromatography.” 2014. Masters Thesis, University of Alberta. Accessed April 11, 2021. https://era.library.ualberta.ca/files/cth83kz515.

MLA Handbook (7^th Edition):

Zhang, Le. “Functionalized Bead Based Microchip for Immunoassay and Virus Immuno-affinity Chromatography.” 2014. Web. 11 Apr 2021.

Vancouver:

Zhang L. Functionalized Bead Based Microchip for Immunoassay and Virus Immuno-affinity Chromatography. [Internet] [Masters thesis]. University of Alberta; 2014. [cited 2021 Apr 11]. Available from: https://era.library.ualberta.ca/files/cth83kz515.

Council of Science Editors:

Zhang L. Functionalized Bead Based Microchip for Immunoassay and Virus Immuno-affinity Chromatography. [Masters Thesis]. University of Alberta; 2014. Available from: https://era.library.ualberta.ca/files/cth83kz515

Oregon State University

5. Cole, Leland Gordon. The apparent energies of the nitrogen - nitrogen and the carbon - carbon bonds and the determinations of the heats of combustion of hydrazobenzene and hippuric acid.

Degree: PhD, Chemistry, 1947, Oregon State University

URL: http://hdl.handle.net/1957/53616

Subjects/Keywords: Chemical affinity

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APA (6^th Edition):

Cole, L. G. (1947). The apparent energies of the nitrogen - nitrogen and the carbon - carbon bonds and the determinations of the heats of combustion of hydrazobenzene and hippuric acid. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/53616

Chicago Manual of Style (16^th Edition):

Cole, Leland Gordon. “The apparent energies of the nitrogen - nitrogen and the carbon - carbon bonds and the determinations of the heats of combustion of hydrazobenzene and hippuric acid.” 1947. Doctoral Dissertation, Oregon State University. Accessed April 11, 2021. http://hdl.handle.net/1957/53616.

MLA Handbook (7^th Edition):

Cole, Leland Gordon. “The apparent energies of the nitrogen - nitrogen and the carbon - carbon bonds and the determinations of the heats of combustion of hydrazobenzene and hippuric acid.” 1947. Web. 11 Apr 2021.

Vancouver:

Cole LG. The apparent energies of the nitrogen - nitrogen and the carbon - carbon bonds and the determinations of the heats of combustion of hydrazobenzene and hippuric acid. [Internet] [Doctoral dissertation]. Oregon State University; 1947. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/1957/53616.

Council of Science Editors:

Cole LG. The apparent energies of the nitrogen - nitrogen and the carbon - carbon bonds and the determinations of the heats of combustion of hydrazobenzene and hippuric acid. [Doctoral Dissertation]. Oregon State University; 1947. Available from: http://hdl.handle.net/1957/53616

Oregon State University

6. Clark, Stacey L. (Stacey Lynn). RNA and DNA aptamers as affinity stationary phases for liquid chromatography and capillary electrochromatography.

Degree: PhD, Chemistry, 2004, Oregon State University

URL: http://hdl.handle.net/1957/29361

Subjects/Keywords: Affinity chromatography

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APA (6^th Edition):

Clark, S. L. (. L. (2004). RNA and DNA aptamers as affinity stationary phases for liquid chromatography and capillary electrochromatography. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/29361

Chicago Manual of Style (16^th Edition):

Clark, Stacey L (Stacey Lynn). “RNA and DNA aptamers as affinity stationary phases for liquid chromatography and capillary electrochromatography.” 2004. Doctoral Dissertation, Oregon State University. Accessed April 11, 2021. http://hdl.handle.net/1957/29361.

MLA Handbook (7^th Edition):

Clark, Stacey L (Stacey Lynn). “RNA and DNA aptamers as affinity stationary phases for liquid chromatography and capillary electrochromatography.” 2004. Web. 11 Apr 2021.

Vancouver:

Clark SL(L. RNA and DNA aptamers as affinity stationary phases for liquid chromatography and capillary electrochromatography. [Internet] [Doctoral dissertation]. Oregon State University; 2004. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/1957/29361.

Council of Science Editors:

Clark SL(L. RNA and DNA aptamers as affinity stationary phases for liquid chromatography and capillary electrochromatography. [Doctoral Dissertation]. Oregon State University; 2004. Available from: http://hdl.handle.net/1957/29361

KTH

7. Remnestål, Julia. Expression and distribution of transcription factors NPAS3 och RFX3 in Alzheimer's disease.

Degree: Biotechnology (BIO), 2015, KTH

URL: http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-173120

►

Alzheimers sjukdom (AD) är den vanligaste formen av demens och påverkar miljontals människor världen över. Förekomst av senil plack och tangles in hjärnan hos… (more)

▼

Alzheimers sjukdom (AD) är den vanligaste formen av demens och påverkar miljontals människor världen över. Förekomst av senil plack och tangles in hjärnan hos personer med AD har varit ett känt faktum sedan början av 1900-talet. Flera studier gjorda under de senaste åren har påvisat en kopplling mellan AD och Diabetes, då försämrad insulin-signalering och nedbrytning av glukos påverkar flera av de molekylära förändringar som uppvisas i AD. I det här projektet använde vi oss av immunofluorescence för att studera uttryck och lokalisering av två transkriptionsfaktorer involverade i nedbrytning av glukos; NPAS3 och RFX3, i hjärnbarken hos patienter med AD, Lewykroppsdemens (DLB) hälsosamma kontroller. Intensiteten på infärgningen av NPAS3 och RFX3 visade inte mängden protein och kvantifierades med algoritmer skrivna i ImageJ. Infärgningen av NPAS3 och RFX3 visade inte på någon signifikant skilland mellan de tre kohorterna och för att förstå mer om deras roll i nedbrytningen av glukos och om det kan påverka AD, måste både infärgnings- och kvantifieringsprocesserna använda i detta projekt optimeras.

Alzheimer's disease (AD) is the most common form of senile dementia. affecting millions o people worldwide. Beta amyloid plaques and neurofibrillary tangles are known to be part of AD pathology since the early nineteen hundreds. In recent years evidence showing that impairments in glucose metabolism can initiate plaque- and tangle formation, as well as several of the other degenerative mechanisms taking place in the AD brain, suggests a potential connection between Alzheimer's disease and Diabetes. Here we used immunofluorescence to study expression of two transcription factors involved in regulation of glucose metabolism; NPAS3 and RFX3. Expression of NPAS3 and RFX3 was investigated in temporal cortex from patients with AD, Dementia with Lewy bodies (DLB) and non-demented age matched controls. The intensity of the immunofluorescent stainings was assumed to be proportional to the amount of stained protein and was quantified in ImageJ. This explorative study did not reveal a significant difference between groups and staining- an quantification procedures would have to be optimized in order to get a clearer understanding about the role of NPAS3 and RFX3 in glucose metabolism, and if that could affect the progression of AD.

Subjects/Keywords: Affinity proteomics; Alzheimer's disease

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APA (6^th Edition):

Remnestål, J. (2015). Expression and distribution of transcription factors NPAS3 och RFX3 in Alzheimer's disease. (Thesis). KTH. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-173120

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Remnestål, Julia. “Expression and distribution of transcription factors NPAS3 och RFX3 in Alzheimer's disease.” 2015. Thesis, KTH. Accessed April 11, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-173120.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Remnestål, Julia. “Expression and distribution of transcription factors NPAS3 och RFX3 in Alzheimer's disease.” 2015. Web. 11 Apr 2021.

Vancouver:

Remnestål J. Expression and distribution of transcription factors NPAS3 och RFX3 in Alzheimer's disease. [Internet] [Thesis]. KTH; 2015. [cited 2021 Apr 11]. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-173120.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Remnestål J. Expression and distribution of transcription factors NPAS3 och RFX3 in Alzheimer's disease. [Thesis]. KTH; 2015. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-173120

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Edinburgh

8. Cruickshank, Faye Louise. Application of an affinity chromatography toolbox to drug repurposing for cancer therapeutics.

Degree: PhD, 2016, University of Edinburgh

URL: http://hdl.handle.net/1842/16162

► Phenotypic screening of drug molecules relies on the generation of a specific response; however the means by which this is elicited often remains unknown. Affinity… (more)

▼ Phenotypic screening of drug molecules relies on the generation of a specific response; however the means by which this is elicited often remains unknown. Affinity chromatography is a valuable tool in the discovery of drug binding partners and may even allow the elucidation of the wider interactome of the initial drug target. The introduction of easily cleavable linkers and affinity-independent elution protocols to affinity chromatography is of current interest, since they render the technique much more adaptable with respect to the characterisation of biologically active species of interest. This thesis details the application of a novel azobenzene linker developed by the Hulme group for use in affinity-independent chromatography. The first chapter reviews recent developments in affinity chromatography and describes the synthesis of an affinity linker toolbox with both affinity-dependent and affinity-independent linkers. These linkers are functionalised with an azide moiety for use in CuAAC coupling to alkynyl derivatives of bioactive small molecules and have been modified to include photoreactive groups giving a series of linkers for use in the identification of less abundant, or low affinity, proteins. The first drug investigated, anisomycin (ANS), is a small molecule which was initially introduced as an antibiotic drug (Flagecidin). At nanomolar concentrations ANS has been shown to affect the mitogen activated protein kinase (MAPK) pathways; downstream effects of these pathways are thought to play a role in a range of pathological disorders such as Alzheimer’s disease, cancer and spinal muscular atrophy (SMA). ANS is thus a candidate for drug repurposing. Although the downstream effects of MAPK/SAPK pathway activation induced by anisomycin are well-documented, the cellular target has yet to be revealed. Previous work by the Hulme group has shown that the N-propargyl anisomycin derivative (I) retains the biological activity of the lead compound ANS. Thus to evaluate the cellular protein targets, N-propargyl ANS (I) was coupled onto the linker toolbox to create an ANS affinity probe library as described in chapter 2. The second drug investigated, fingolimod, was introduced as an immunomodulating drug (Glienya) for the treatment of multiple sclerosis (MS). This small molecule has also been shown to have anti-cancer properties in a range of cancer cell lines; however the precise mechanism by which this is effected is unknown. Literature precedent shows that terminal modification of fingolimod generates analogues which still retain biological activity. Thus a novel fingolimod alkyne derivative (II) was synthesised and used to create an affinity probe library as described in chapter 3. Chapter 4 describes affinity pull-down experiments conducted with the aim of finding the protein target(s) of ANS and fingolimod, using the affinity probe libraries generated in chapters 2 and 3. This chapter concludes with a discussion of the implications of these findings and directions for future study.

Subjects/Keywords: 547; azobenzene linker; affinity-independent chromatography; affinity chromatography; anisomycin; fingolimod

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APA (6^th Edition):

Cruickshank, F. L. (2016). Application of an affinity chromatography toolbox to drug repurposing for cancer therapeutics. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/16162

Chicago Manual of Style (16^th Edition):

Cruickshank, Faye Louise. “Application of an affinity chromatography toolbox to drug repurposing for cancer therapeutics.” 2016. Doctoral Dissertation, University of Edinburgh. Accessed April 11, 2021. http://hdl.handle.net/1842/16162.

MLA Handbook (7^th Edition):

Cruickshank, Faye Louise. “Application of an affinity chromatography toolbox to drug repurposing for cancer therapeutics.” 2016. Web. 11 Apr 2021.

Vancouver:

Cruickshank FL. Application of an affinity chromatography toolbox to drug repurposing for cancer therapeutics. [Internet] [Doctoral dissertation]. University of Edinburgh; 2016. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/1842/16162.

Council of Science Editors:

Cruickshank FL. Application of an affinity chromatography toolbox to drug repurposing for cancer therapeutics. [Doctoral Dissertation]. University of Edinburgh; 2016. Available from: http://hdl.handle.net/1842/16162

University of California – Berkeley

9. Pham, Tiffany Anhtu. Development of a Thorium Complex for Radiotherapeutic Applications.

Degree: Chemistry, 2014, University of California – Berkeley

URL: http://www.escholarship.org/uc/item/2w76f60h

► Chapter 1. A brief history of the use of ionizing radiation for cancer therapy is presented, as the motivation for modern clinical investigations on alpha… (more)

▼ Chapter 1. A brief history of the use of ionizing radiation for cancer therapy is presented, as the motivation for modern clinical investigations on alpha therapy and the use and development of bifunctional chelators in targeted medicine. Recent studies on targeted therapy with alpha-emitters, such as 223Ra and 227Th, are described. The chemistry of Th(IV) is presented with a survey of important coordination compounds, with special emphasis on those developed by the Raymond group.Chapter 2. The rational design of a ligand as a suitable bifunctional chelator for 227Th therapy is described in the context of ligands developed by the Raymond group for actinide sequestration. A novel Φ ligand topology incorporating macrocyclic and pendant terephthalamide binding groups aims to address the kinetic and thermodynamic requirements of targeted alpha therapy. The syntheses of the model ligands Φ(2,2)moeTAM and Φ(3,3)moeTAM are detailed, from which a derivative of Φ(2,2)moeTAM is developed. Functionalization of a pendant terephthalamide of the model ligand gives the asymmetric bifunctional chelate Φ(2,2)NBuTAM via the strategic use of protecting groups.Chapter 3. The evaluation of the solution thermodynamic behavior of Φ(2,2)moeTAM is extensively detailed. The protonation constants of the ligand and its affinity for Th(IV) is measured by spectrophotometric titration. The thorium complex was found to have a remarkably high thermodynamic stability, with a formation constant of 1054, surpassing the stabilities of previously measured complexes. Efforts to elucidate the effects of the Φ topology on the coordination of Th(IV) using thermodynamic studies of the linear analog 3,4,3-LiMeTAM as well as the shape analyses of the crystal structure of Th[Φ(2,2)moeTAM] are described. The surprising geometry adopted by Φ(2,2)moeTAM in the thorium complex relative to its structure as the free ligand is further investigated with DFT studies.Chapter 4. While the measurement of the thermodynamic parameters of the thorium complex provides an assessment of its inertness, its formation kinetics are also of paramount importance due to the inherent time sensitivity of radiotherapeutic agents. Difficulties encountered in preliminary kinetic experiments with existing octadentate ligands and Φ(2,2)moeTAM lead to the use of indirect kinetics. Dye-displacement kinetic studies with the Φ ligands, 3,4,3-LiMeTAM, and the aminocarboxylic acid ligands provide a useful qualitative comparison of the rates of complexation of these ligands with Th(IV). Φ(2,2)moeTAM is observed to be a much more rapid chelator than the prevailing DTPA and DOTA, and its association (a second-order reaction with a rate constant of k2 = 1.8(1) x 104 M-1s-1) can be directly measured using stopped-flow kinetics. Chapter 5. The application of Φ(2,2)moeTAM toward the coordination of the lanthanide analogs cerium and praseodymium is presented. The crystal structure of the Ce(IV) complex with Φ(2,2)moeTAM is isomorphous to…

Subjects/Keywords: Chemistry; Affinity; Cerium; Macrocycle; Radiotherapy; Thermodynamic; Thorium

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APA (6^th Edition):

Pham, T. A. (2014). Development of a Thorium Complex for Radiotherapeutic Applications. (Thesis). University of California – Berkeley. Retrieved from http://www.escholarship.org/uc/item/2w76f60h

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pham, Tiffany Anhtu. “Development of a Thorium Complex for Radiotherapeutic Applications.” 2014. Thesis, University of California – Berkeley. Accessed April 11, 2021. http://www.escholarship.org/uc/item/2w76f60h.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pham, Tiffany Anhtu. “Development of a Thorium Complex for Radiotherapeutic Applications.” 2014. Web. 11 Apr 2021.

Vancouver:

Pham TA. Development of a Thorium Complex for Radiotherapeutic Applications. [Internet] [Thesis]. University of California – Berkeley; 2014. [cited 2021 Apr 11]. Available from: http://www.escholarship.org/uc/item/2w76f60h.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pham TA. Development of a Thorium Complex for Radiotherapeutic Applications. [Thesis]. University of California – Berkeley; 2014. Available from: http://www.escholarship.org/uc/item/2w76f60h

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Penn State University

10. Olcese, Nicole Rose. Beginning Teachers Finding Support Through an Online Teacher Network: Affinity Lear-NING.

Degree: 2014, Penn State University

URL: https://submit-etda.libraries.psu.edu/catalog/22727

► Teacher attrition remains a concern in the field of education; while this is not a new problem, the reasons why teachers tend to leave the… (more)

▼ Teacher attrition remains a concern in the field of education; while this is not a new problem, the reasons why teachers tend to leave the profession are concerning. Recently, the Carnegie Foundation for the Advancement of Teaching (2014) found that nearly one third of all teachers leave the profession within their first three years; many cited a lack of administrative and professional support as a major factor in their decision to seek alternative employment. “The problem takes many forms, including the feeling of being isolated from colleagues, scant feedback on performance, poor professional development, and insufficient emotional backing by administrators” (p.7). While there is no replacement for a solid teacher induction program, it is clear that teacher educators need to find alternative and expansive ways to provide intellectual and emotional support to new teachers as they enter the profession, helping them focus not only on their pedagogical and content knowledge skills, but also on nurturing their emerging and constantly evolving teacher identifications. One way that teachers are able to achieve this level of additional support on their own, bridging the gap from the University to their initial in service positions (supplementing what can sometimes be less than ideal professional development), is to turn to online teacher communities, or what I term online teacher affinity spaces. The purpose of this study was to investigate the types of support beginning teachers perceive they obtain from voluntary membership within a particular online teacher affinity space called The English Companion Ning (EC Ning). Using a qualitative ethnographic approach, I sought to identify the types of discursive practices that emerge as beginning teachers participate in the content-specific online space. It incorporates sociocultural and social learning theories (Gee, 2006; Lave and Wenger, 1991; Wenger 1998) to explore how beginning teachers, often considered legitimate peripheral participants, display patterns of membership and interaction, as well as how they position and identify themselves as beginning teachers within the discussions they engage in. The results of this study suggest the potential of online teacher affinity spaces to support beginning teachers, both professionally and emotionally. A salient finding is that beginning teachers tend to take up various degrees of participation in the EC Ning and this can be closely tied to the levels of support they experience at their schools of employment. In addition, the EC Ning displayed elements of affinity space that provided beginning teachers the opportunity to conduct important identity formation and experimentation. The utilization and implementation of these types of spaces as supplemental support, as early on as in teacher education programs, have the potential to provide beginning teachers with the reinforcements they need in order to survive and thrive during their first years of teaching. Advisors/Committee Members: Jamie Myers, Dissertation Advisor/Co-Advisor, Jamie Myers, Committee Chair/Co-Chair, Priya Sharma, Committee Member, Anne Whitney, Committee Member, James F Nolan Jr., Committee Member.

Subjects/Keywords: Affinity Spaces; Teacher Education; Online Social Networks

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Olcese, N. R. (2014). Beginning Teachers Finding Support Through an Online Teacher Network: Affinity Lear-NING. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/22727

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Olcese, Nicole Rose. “Beginning Teachers Finding Support Through an Online Teacher Network: Affinity Lear-NING.” 2014. Thesis, Penn State University. Accessed April 11, 2021. https://submit-etda.libraries.psu.edu/catalog/22727.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Olcese, Nicole Rose. “Beginning Teachers Finding Support Through an Online Teacher Network: Affinity Lear-NING.” 2014. Web. 11 Apr 2021.

Vancouver:

Olcese NR. Beginning Teachers Finding Support Through an Online Teacher Network: Affinity Lear-NING. [Internet] [Thesis]. Penn State University; 2014. [cited 2021 Apr 11]. Available from: https://submit-etda.libraries.psu.edu/catalog/22727.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Olcese NR. Beginning Teachers Finding Support Through an Online Teacher Network: Affinity Lear-NING. [Thesis]. Penn State University; 2014. Available from: https://submit-etda.libraries.psu.edu/catalog/22727

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Colorado State University

11. Berquam, Lori M. Affinity development in undergraduate students at a large research institution.

Degree: PhD, Education, 2013, Colorado State University

URL: http://hdl.handle.net/10217/78823

► Public institutions of higher education have faced repeated financial reductions over the past decade. The cumulative effect of reductions has necessitated public institutions re-examine funding… (more)

Subjects/Keywords: affinity; alumni; satisfaction; high impact practice

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APA (6^th Edition):

Berquam, L. M. (2013). Affinity development in undergraduate students at a large research institution. (Doctoral Dissertation). Colorado State University. Retrieved from http://hdl.handle.net/10217/78823

Chicago Manual of Style (16^th Edition):

Berquam, Lori M. “Affinity development in undergraduate students at a large research institution.” 2013. Doctoral Dissertation, Colorado State University. Accessed April 11, 2021. http://hdl.handle.net/10217/78823.

MLA Handbook (7^th Edition):

Berquam, Lori M. “Affinity development in undergraduate students at a large research institution.” 2013. Web. 11 Apr 2021.

Vancouver:

Berquam LM. Affinity development in undergraduate students at a large research institution. [Internet] [Doctoral dissertation]. Colorado State University; 2013. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/10217/78823.

Council of Science Editors:

Berquam LM. Affinity development in undergraduate students at a large research institution. [Doctoral Dissertation]. Colorado State University; 2013. Available from: http://hdl.handle.net/10217/78823

Boston University

12. Ataca, Sila. Biophysical characterization of affinity maturation in the human response to anthrax vaccine.

Degree: PhD, Microbiology, 2018, Boston University

URL: http://hdl.handle.net/2144/32958

► Affinity maturation increases the affinity of B-cell derived antibodies to their cognate antigens. In this study, we characterized the kinetic, structural, dynamic and thermodynamic evolution… (more)

▼ Affinity maturation increases the affinity of B-cell derived antibodies to their cognate antigens. In this study, we characterized the kinetic, structural, dynamic and thermodynamic evolution of antibodies during affinity maturation. Through single B-cell cell sorting, paired heavy and light chain sequencing, phylogenetic analysis, antibody expression, and physicochemical characterization, we were able to longitudinally analyze the stages of affinity maturation of anti-PA (B.anthracis protective antigen) antibodies. Following repeated immunizations, we observed up to an 10,000-fold increase in antibody affinity, mainly through a decrease in the off-rates. For detailed maturation analysis, we chose three antibodies lying along a single clonal branch – the clone’s unmutated common ancestor (UCA), a medium affinity antibody (MAAb) appearing after second immunization, and a high-affinity antibody (HAAb) appearing after third immunization. Most of the mutations that occur between the UCA and HAAb resulted in key changes to structural conformation. In particular, mutations change residues in the CDR-H3 region inducing the folding of the CDR-loops into a conformation that is more complementary to PA. This advantageous new antibody conformation is preserved in the unbound state, indicating that though the UCA and MAAb appear to use an induced fit and/or conformational selection mechanism, the HAAb is more rigidly lock-and-key. Thermodynamic results support this interpretation. In the first maturation step from UCA to MAAb, enthalpic improvement indicates optimization of noncovalent interactions. The second step from MAAb to HAAb predominantly involves entropic improvement by which the advantageous conformation made accessible in the first step is made more dominant via the narrowing of effectively accessible conformations, which allows better contact with PA. This is also reflected by a less significant improvement in the enthalpic component of PA-binding. Studies examining the evolving protein-dynamic characteristics further support this interpretation. In summary, we observed that a single energetic component is not responsible for increased affinity in the maturation pathways we studied. From UCA to MAAb, affinity increases through optimization of noncovalent interactions. From MAAb to HAAb, affinity increase is achieved through changes that stabilize the favorable conformation in the unbound state. A better understanding of affinity maturation can have implications for antibody engineering and vaccine development. Advisors/Committee Members: Kepler, Thomas B. (advisor), Gursky, Olga (advisor).

Subjects/Keywords: Microbiology; Anthrax; Antibody; Vaccine; Affinity maturation

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APA (6^th Edition):

Ataca, S. (2018). Biophysical characterization of affinity maturation in the human response to anthrax vaccine. (Doctoral Dissertation). Boston University. Retrieved from http://hdl.handle.net/2144/32958

Chicago Manual of Style (16^th Edition):

Ataca, Sila. “Biophysical characterization of affinity maturation in the human response to anthrax vaccine.” 2018. Doctoral Dissertation, Boston University. Accessed April 11, 2021. http://hdl.handle.net/2144/32958.

MLA Handbook (7^th Edition):

Ataca, Sila. “Biophysical characterization of affinity maturation in the human response to anthrax vaccine.” 2018. Web. 11 Apr 2021.

Vancouver:

Ataca S. Biophysical characterization of affinity maturation in the human response to anthrax vaccine. [Internet] [Doctoral dissertation]. Boston University; 2018. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/2144/32958.

Council of Science Editors:

Ataca S. Biophysical characterization of affinity maturation in the human response to anthrax vaccine. [Doctoral Dissertation]. Boston University; 2018. Available from: http://hdl.handle.net/2144/32958

University of Illinois – Chicago

13. Gorman, Kevin Thomas. Designer Affinity Reagents for Biomarker Detection.

Degree: 2017, University of Illinois – Chicago

URL: http://hdl.handle.net/10027/21956

► Biomarkers are molecules whose presence is indicative of some biological phenomena, such as disease or infection. Since their discovery, biomarkers have played a vital role… (more)

Subjects/Keywords: phage-display; affinity reagents; sandwich assay

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APA (6^th Edition):

Gorman, K. T. (2017). Designer Affinity Reagents for Biomarker Detection. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/21956

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gorman, Kevin Thomas. “Designer Affinity Reagents for Biomarker Detection.” 2017. Thesis, University of Illinois – Chicago. Accessed April 11, 2021. http://hdl.handle.net/10027/21956.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gorman, Kevin Thomas. “Designer Affinity Reagents for Biomarker Detection.” 2017. Web. 11 Apr 2021.

Vancouver:

Gorman KT. Designer Affinity Reagents for Biomarker Detection. [Internet] [Thesis]. University of Illinois – Chicago; 2017. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/10027/21956.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gorman KT. Designer Affinity Reagents for Biomarker Detection. [Thesis]. University of Illinois – Chicago; 2017. Available from: http://hdl.handle.net/10027/21956

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Jönköping University

14. Landeros, Victor Manuel Reynoso. The Enchilada effect: Do ethnocentrism,affinity & PCI influence the COO effect onconsumers’ foreign product attribute andtype preferences?.

Degree: Marketing and Logistics, 2011, Jönköping University

URL: http://urn.kb.se/resolve?urn=urn:nbn:se:hj:diva-18391

► Purpose: To identify the relevance ethnocentrism, affinity and product country-image (the three theory effect affectionately called “the enchilada effect” by the authors) have on… (more)

Subjects/Keywords: COO; CETSCALE; consumer behavior; Mexico; conjoint; affinity

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APA (6^th Edition):

Landeros, V. M. R. (2011). The Enchilada effect: Do ethnocentrism,affinity & PCI influence the COO effect onconsumers’ foreign product attribute andtype preferences?. (Thesis). Jönköping University. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:hj:diva-18391

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Landeros, Victor Manuel Reynoso. “The Enchilada effect: Do ethnocentrism,affinity & PCI influence the COO effect onconsumers’ foreign product attribute andtype preferences?.” 2011. Thesis, Jönköping University. Accessed April 11, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:hj:diva-18391.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Landeros, Victor Manuel Reynoso. “The Enchilada effect: Do ethnocentrism,affinity & PCI influence the COO effect onconsumers’ foreign product attribute andtype preferences?.” 2011. Web. 11 Apr 2021.

Vancouver:

Landeros VMR. The Enchilada effect: Do ethnocentrism,affinity & PCI influence the COO effect onconsumers’ foreign product attribute andtype preferences?. [Internet] [Thesis]. Jönköping University; 2011. [cited 2021 Apr 11]. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:hj:diva-18391.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Landeros VMR. The Enchilada effect: Do ethnocentrism,affinity & PCI influence the COO effect onconsumers’ foreign product attribute andtype preferences?. [Thesis]. Jönköping University; 2011. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:hj:diva-18391

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Victoria

15. Garnett, Graham. Substitutions of sulfonatocalix[4]arenes that lead to applications in biomolecular recognition and give rise to novel self-association phenomena.

Degree: Department of Chemistry, 2014, University of Victoria

URL: http://hdl.handle.net/1828/5810

► The epigenetic post-translational modifications (PTMs) of histone proteins are numerous and have an important role in regulating cellular development. Molecular recognition elements that can bind… (more)

Subjects/Keywords: Kme3; calixarene; self-association; affinity chromatography

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APA (6^th Edition):

Garnett, G. (2014). Substitutions of sulfonatocalix[4]arenes that lead to applications in biomolecular recognition and give rise to novel self-association phenomena. (Masters Thesis). University of Victoria. Retrieved from http://hdl.handle.net/1828/5810

Chicago Manual of Style (16^th Edition):

Garnett, Graham. “Substitutions of sulfonatocalix[4]arenes that lead to applications in biomolecular recognition and give rise to novel self-association phenomena.” 2014. Masters Thesis, University of Victoria. Accessed April 11, 2021. http://hdl.handle.net/1828/5810.

MLA Handbook (7^th Edition):

Garnett, Graham. “Substitutions of sulfonatocalix[4]arenes that lead to applications in biomolecular recognition and give rise to novel self-association phenomena.” 2014. Web. 11 Apr 2021.

Vancouver:

Garnett G. Substitutions of sulfonatocalix[4]arenes that lead to applications in biomolecular recognition and give rise to novel self-association phenomena. [Internet] [Masters thesis]. University of Victoria; 2014. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/1828/5810.

Council of Science Editors:

Garnett G. Substitutions of sulfonatocalix[4]arenes that lead to applications in biomolecular recognition and give rise to novel self-association phenomena. [Masters Thesis]. University of Victoria; 2014. Available from: http://hdl.handle.net/1828/5810

California State University – Northridge

16. Tadle, Donna. Culture and detection of paternal investment through facial cues.

Degree: MA, Department of Psychology, 2012, California State University – Northridge

URL: http://hdl.handle.net/10211.2/1148

► This study examined whether the ???in-group advantage??? (Elfenbein & Ambady, 2002a) exists in Caucasian American and African American women???s ability to detect cues of paternal… (more)

Subjects/Keywords: affinity for children; Dissertations, Academic – CSUN – Psychology.

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APA (6^th Edition):

Tadle, D. (2012). Culture and detection of paternal investment through facial cues. (Masters Thesis). California State University – Northridge. Retrieved from http://hdl.handle.net/10211.2/1148

Chicago Manual of Style (16^th Edition):

Tadle, Donna. “Culture and detection of paternal investment through facial cues.” 2012. Masters Thesis, California State University – Northridge. Accessed April 11, 2021. http://hdl.handle.net/10211.2/1148.

MLA Handbook (7^th Edition):

Tadle, Donna. “Culture and detection of paternal investment through facial cues.” 2012. Web. 11 Apr 2021.

Vancouver:

Tadle D. Culture and detection of paternal investment through facial cues. [Internet] [Masters thesis]. California State University – Northridge; 2012. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/10211.2/1148.

Council of Science Editors:

Tadle D. Culture and detection of paternal investment through facial cues. [Masters Thesis]. California State University – Northridge; 2012. Available from: http://hdl.handle.net/10211.2/1148

University of Tennessee – Knoxville

17. Qian, Chen. Development of MS-based proteomics approaches to examine metabolic pathways and protein:protein interactions in microbial systems.

Degree: 2017, University of Tennessee – Knoxville

URL: https://trace.tennessee.edu/utk_graddiss/4709

► The proteome is perhaps the most functional operating machinery for almost all biological processes, serving as the bridge to link the genome and phenotypes. The… (more)

▼ The proteome is perhaps the most functional operating machinery for almost all biological processes, serving as the bridge to link the genome and phenotypes. The proteome undergoes dynamic changes in terms of the abundance or interactions, responding to the environmental stimuli. Understanding this dynamic of protein alterations is the key to delineate critical biological mechanisms. Mass-spectrometry-based proteomics is a powerful tool to systematically monitor the heterogeneous alterations of the proteome, including the changes of abundance, modifications and interactions. In this dissertation, a research project was built upon current proteomics approaches to solve the issues regarding to the sample preparation and data analysis that would help propel this approach to better address certain questions in environmental microbiology and molecular biology. In the first study, we have designed an experimental method that efficiently removes humic acids prior to proteolytic peptide measurement, thus addressing one major challenge associated with soil microbiome proteome extraction and subsequent MS measurement. The second study was aimed at employing advanced proteomics approaches to better understand the microbial drivers of environmental mercury processes by using two model organisms: Geobacter sulfurreducens PCA and Desulfovibrio desulfuricans ND132. Our results elucidated the global proteome impacts caused by the deletion of mercury methylation essential genes hgcAB and revealed that deletion of hgcAB genes did not show significant impact on the microbial response to mercury addition. The third study focused on optimizing MS-based proteome approaches for characterizing protein-protein interactions. By coupling the rapid crosslinking procedure, affinity enrichments and high-performance MS measurements, protein-protein interaction can be captured and interrogated in a living cell by a time-resolved manner. Overall, the methods and results presented in this dissertation not only provides an enhanced sample preparation methodology for intractable samples (such as soils), but also demonstrates how this systems-biology approach can be utilized to characterize the basics of microbial physiology at a global proteome level as well as drilling down into specific proteins interactions. The optimized methods and experimental/bioinformatics techniques described in this dissertation should be broadly extendable to proteome characterization/protein interaction examination in various systems.

Subjects/Keywords: shotgun-proteomics; environmental proteomics; affinity proteomics; Biotechnology

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APA (6^th Edition):

Qian, C. (2017). Development of MS-based proteomics approaches to examine metabolic pathways and protein:protein interactions in microbial systems. (Doctoral Dissertation). University of Tennessee – Knoxville. Retrieved from https://trace.tennessee.edu/utk_graddiss/4709

Chicago Manual of Style (16^th Edition):

Qian, Chen. “Development of MS-based proteomics approaches to examine metabolic pathways and protein:protein interactions in microbial systems.” 2017. Doctoral Dissertation, University of Tennessee – Knoxville. Accessed April 11, 2021. https://trace.tennessee.edu/utk_graddiss/4709.

MLA Handbook (7^th Edition):

Qian, Chen. “Development of MS-based proteomics approaches to examine metabolic pathways and protein:protein interactions in microbial systems.” 2017. Web. 11 Apr 2021.

Vancouver:

Qian C. Development of MS-based proteomics approaches to examine metabolic pathways and protein:protein interactions in microbial systems. [Internet] [Doctoral dissertation]. University of Tennessee – Knoxville; 2017. [cited 2021 Apr 11]. Available from: https://trace.tennessee.edu/utk_graddiss/4709.

Council of Science Editors:

Qian C. Development of MS-based proteomics approaches to examine metabolic pathways and protein:protein interactions in microbial systems. [Doctoral Dissertation]. University of Tennessee – Knoxville; 2017. Available from: https://trace.tennessee.edu/utk_graddiss/4709

University of Minnesota

18. Szlag, Victoria. Linear Polymer Affinity Agents for the Intrinsic SERS Detection of Food Safety Targets.

Degree: PhD, Chemistry, 2018, University of Minnesota

URL: http://hdl.handle.net/11299/216329

► This dissertation explores the use of polymer affinity agents for the surface-enhanced Raman spectroscopy (SERS) detection of food safety targets. First, current molecular motifs used… (more)

▼ This dissertation explores the use of polymer affinity agents for the surface-enhanced Raman spectroscopy (SERS) detection of food safety targets. First, current molecular motifs used as affinity agents in intrinsic surface-enhanced Raman spectroscopy (SERS) sensors are reviewed. By comparing antibody, aptamer, small molecule, and polymer affinity agents, the largely unresearched potential of polymer affinity agents (chemical customization, tunable length, ease of production, opportunity for multiplexing) is highlighted. The first proof of concept work of this dissertation targets the detection of the bioterror agent, ricin B-chain (RBC) in water and liquid food matrices. An N-acetyl-galactosamine glycopolymer capture layer was designed and applied to create a SERS sensor. The sensing scheme’s detection limit (20 ng/mL) is well below that of the predicted oral exposure limit. The RBC was detected in two types of juice, and a computed normal Raman spectrum of the glycomonomer supports polymer–RBC intermolecular interactions at the functional group level. Subsequent work focuses on the translation of this sensing scheme from the detection of proteins to the detection of small molecules relevant to food safety. Because interactions between a small molecule target and a polymer affinity agent are less specific than those that were leveraged in the RBC work, the development of a rapid affinity agent screening method was deemed necessary. A potent carcinogenic metabolite of a fungal pathogen that can infect food and feedstocks, aflatoxin B1 (AFB1), was used as a model target. Seven homopolymers of nitrogen-inclusive poly(N-(2-aminoethyl) methacrylamide) (pAEMA) and their oxygen analogs, poly(2-yydroxyethyl methacrylate) (pHEMA) were synthesized to be evaluated as AFB1 affinity agents based on hypothetical hydrogen bonding interactions and optimal polymer length. An isothermal titration calorimetry (ITC) method was development for rapid affinity agent screening and good agreement was observed between the ITC results and follow-on SERS sensing experiments. A final polymer series (poly(N-acryloyl glycinamide), pNAGA) was designed for the capture of AFB1 and was used to explore the influence of polymer molecular weight (2.0 – 5.2 kDa), attachment chemistry (thiol vs. trithiocarbonate), and order of addition (pre- vs. post- functionalization of the substrate) on the sensitivity of AFB1 detection. The best polymer chain length (pNAGA22), anchoring chemistry (thiol), and polymer/toxin assembly scheme (in-solution) allowed detection of 10 ppb AFB1 in water (below the FDA regulatory limit of 20 ppb), a hundred-fold improvement over SERS sensing without the pNAGA affinity agent. This dissertation concludes with the advantages, disadvantages, and future perspectives of polymers used as analytical affinity agents. Adjustment of surface attachment moieties, the use of crosslinkers with target affinity, and application to other signal transduction mechanisms are emphasized a potential areas for continuing work.

Subjects/Keywords: Affinity Agent; Aflatoxin; Polymer; Ricin; SERS

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APA (6^th Edition):

Szlag, V. (2018). Linear Polymer Affinity Agents for the Intrinsic SERS Detection of Food Safety Targets. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/216329

Chicago Manual of Style (16^th Edition):

Szlag, Victoria. “Linear Polymer Affinity Agents for the Intrinsic SERS Detection of Food Safety Targets.” 2018. Doctoral Dissertation, University of Minnesota. Accessed April 11, 2021. http://hdl.handle.net/11299/216329.

MLA Handbook (7^th Edition):

Szlag, Victoria. “Linear Polymer Affinity Agents for the Intrinsic SERS Detection of Food Safety Targets.” 2018. Web. 11 Apr 2021.

Vancouver:

Szlag V. Linear Polymer Affinity Agents for the Intrinsic SERS Detection of Food Safety Targets. [Internet] [Doctoral dissertation]. University of Minnesota; 2018. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/11299/216329.

Council of Science Editors:

Szlag V. Linear Polymer Affinity Agents for the Intrinsic SERS Detection of Food Safety Targets. [Doctoral Dissertation]. University of Minnesota; 2018. Available from: http://hdl.handle.net/11299/216329

Virginia Tech

19. Stapp, April Marie. 'Occupying' Anarchism and Discovering the Means for Social Justice: Interrogating the Anarchist Turn in 21st Century Social Movements.

Degree: MS, Sociology, 2013, Virginia Tech

URL: http://hdl.handle.net/10919/51116

► The purpose of this thesis is to take the individual on a journey about what it is like to be engaged in radical anti-systemic activism… (more)

Subjects/Keywords: social movements; anarchism; social justice; affinity

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APA (6^th Edition):

Stapp, A. M. (2013). 'Occupying' Anarchism and Discovering the Means for Social Justice: Interrogating the Anarchist Turn in 21st Century Social Movements. (Masters Thesis). Virginia Tech. Retrieved from http://hdl.handle.net/10919/51116

Chicago Manual of Style (16^th Edition):

Stapp, April Marie. “'Occupying' Anarchism and Discovering the Means for Social Justice: Interrogating the Anarchist Turn in 21st Century Social Movements.” 2013. Masters Thesis, Virginia Tech. Accessed April 11, 2021. http://hdl.handle.net/10919/51116.

MLA Handbook (7^th Edition):

Stapp, April Marie. “'Occupying' Anarchism and Discovering the Means for Social Justice: Interrogating the Anarchist Turn in 21st Century Social Movements.” 2013. Web. 11 Apr 2021.

Vancouver:

Stapp AM. 'Occupying' Anarchism and Discovering the Means for Social Justice: Interrogating the Anarchist Turn in 21st Century Social Movements. [Internet] [Masters thesis]. Virginia Tech; 2013. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/10919/51116.

Council of Science Editors:

Stapp AM. 'Occupying' Anarchism and Discovering the Means for Social Justice: Interrogating the Anarchist Turn in 21st Century Social Movements. [Masters Thesis]. Virginia Tech; 2013. Available from: http://hdl.handle.net/10919/51116

University of Bath

20. Crean, Rory. Utilising molecular dynamics simulations to understand and engineer T-cell receptors.

Degree: PhD, 2020, University of Bath

URL: https://researchportal.bath.ac.uk/en/studentthesis/utilising-molecular-dynamics-simulations-to-understand-and-engineer-tcell-receptors(d8c626a7-9b6e-4e6f-8f2c-716b48d14b49).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.805664

► Approximately 90% of all therapeutic targets in the human proteome operate solely inside the cell, making them unavailable for recognition by antibodies which instead bind… (more)

▼ Approximately 90% of all therapeutic targets in the human proteome operate solely inside the cell, making them unavailable for recognition by antibodies which instead bind antigens presented on the exterior of the cell surface. To target the 90%, the human immune system utilises a class of binding proteins known as T-cell receptors (TCRs). These TCRs recognise peptide fragments sourced from proteins produced inside the cell that have subsequently been degraded and transported to the cell surface by the human leukocyte antigen (HLA, pHLA with peptide bound). TCRs are membrane bound and attached to T-cells and use their six complementarity-determining region (CDR) loops to bind antigenic pHLA molecules (i.e. peptides that come from protein antigens). TCR binding to pHLA molecules induces an immune response from the T-cell, which ultimately leads to the antigen presenting cells death. The capability of TCRs to identify antigens which are not naturally expressed on the cell surface (unlike antibodies) has helped drive the development of a new class of therapeutics that consist of a soluble, bispecific TCR engineered to bind a specific antigenic pHLA for the treatment of various diseases (such as cancers and viral infections). Natural TCRs bind with characteristically poor affinities (~μM) and half-lives (~seconds), which are undesirable properties for a therapeutic. The CDR loops on TCRs are therefore normally subjected to affinity maturation to produce TCRs with affinities in ~pM range for their target pHLA. This does however carry a significant risk in terms of safety, as the very large majority of peptides presented by HLA molecules are sourced from endogenous (i.e. healthy) proteins and must not be bound by the TCR in order to avoid the production of an autoimmune response on the healthy cells. These requirements for a highly specific TCR that binds with high affinity to its target pHLA is the primary motivation for this thesis, as herein, fundamental engineering principles for generating TCRs with these properties are determined and protocols to evaluate these properties are developed and demonstrated. This insight is obtained through combinations of structural analysis, molecular dynamics simulations and free energy calculations, providing an atomistic description of how this has occurred for several TCRs. Furthermore, we characterise how different peptide cargo can tune the molecular flexibility of the entire pHLA molecule, including regions distal from the HLA binding site. These findings suggest peptide dependant tuning of the HLA molecule may play a role in regulating the functional outcome of an immune response. Ultimately, this work and the principles identified herein will aid in the rational design of high affinity and high specificity TCRs as therapeutics for various diseases.

Subjects/Keywords: TCR; pHLA; Molecular dynamics; affinity; free energy

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APA (6^th Edition):

Crean, R. (2020). Utilising molecular dynamics simulations to understand and engineer T-cell receptors. (Doctoral Dissertation). University of Bath. Retrieved from https://researchportal.bath.ac.uk/en/studentthesis/utilising-molecular-dynamics-simulations-to-understand-and-engineer-tcell-receptors(d8c626a7-9b6e-4e6f-8f2c-716b48d14b49).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.805664

Chicago Manual of Style (16^th Edition):

Crean, Rory. “Utilising molecular dynamics simulations to understand and engineer T-cell receptors.” 2020. Doctoral Dissertation, University of Bath. Accessed April 11, 2021. https://researchportal.bath.ac.uk/en/studentthesis/utilising-molecular-dynamics-simulations-to-understand-and-engineer-tcell-receptors(d8c626a7-9b6e-4e6f-8f2c-716b48d14b49).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.805664.

MLA Handbook (7^th Edition):

Crean, Rory. “Utilising molecular dynamics simulations to understand and engineer T-cell receptors.” 2020. Web. 11 Apr 2021.

Vancouver:

Crean R. Utilising molecular dynamics simulations to understand and engineer T-cell receptors. [Internet] [Doctoral dissertation]. University of Bath; 2020. [cited 2021 Apr 11]. Available from: https://researchportal.bath.ac.uk/en/studentthesis/utilising-molecular-dynamics-simulations-to-understand-and-engineer-tcell-receptors(d8c626a7-9b6e-4e6f-8f2c-716b48d14b49).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.805664.

Council of Science Editors:

Crean R. Utilising molecular dynamics simulations to understand and engineer T-cell receptors. [Doctoral Dissertation]. University of Bath; 2020. Available from: https://researchportal.bath.ac.uk/en/studentthesis/utilising-molecular-dynamics-simulations-to-understand-and-engineer-tcell-receptors(d8c626a7-9b6e-4e6f-8f2c-716b48d14b49).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.805664

University of Sydney

21. Sifniotis, Vicki. Towards The Development of Biobetter Therapeutic Whole Antibodies .

Degree: 2019, University of Sydney

URL: http://hdl.handle.net/2123/20829

► The development of therapeutic antibodies has been revolutionary in the pharmaceutical industry due to the specificity of antibodies to a biological target and their ability… (more)

Subjects/Keywords: antibody; protein stability; formulation; aggregation; biopharmaceutic; affinity

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APA (6^th Edition):

Sifniotis, V. (2019). Towards The Development of Biobetter Therapeutic Whole Antibodies . (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/20829

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sifniotis, Vicki. “Towards The Development of Biobetter Therapeutic Whole Antibodies .” 2019. Thesis, University of Sydney. Accessed April 11, 2021. http://hdl.handle.net/2123/20829.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sifniotis, Vicki. “Towards The Development of Biobetter Therapeutic Whole Antibodies .” 2019. Web. 11 Apr 2021.

Vancouver:

Sifniotis V. Towards The Development of Biobetter Therapeutic Whole Antibodies . [Internet] [Thesis]. University of Sydney; 2019. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/2123/20829.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sifniotis V. Towards The Development of Biobetter Therapeutic Whole Antibodies . [Thesis]. University of Sydney; 2019. Available from: http://hdl.handle.net/2123/20829

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Cincinnati

22. Reich, Samuel. To Affinity and Beyond: The Sound of Diatonic Positions.

Degree: DMA, College-Conservatory of Music: Piano, 2020, University of Cincinnati

URL: http://rave.ohiolink.edu/etdc/view?acc_num=ucin1595849229880202

► This document seeks to elucidate the perceptual quality of a note’s position in the diatonic scale, regardless of its distance from a centric pitch. While… (more)

Subjects/Keywords: Music; Diatonic Position; Affinity; Scale Theory; Bach

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APA (6^th Edition):

Reich, S. (2020). To Affinity and Beyond: The Sound of Diatonic Positions. (Doctoral Dissertation). University of Cincinnati. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=ucin1595849229880202

Chicago Manual of Style (16^th Edition):

Reich, Samuel. “To Affinity and Beyond: The Sound of Diatonic Positions.” 2020. Doctoral Dissertation, University of Cincinnati. Accessed April 11, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1595849229880202.

MLA Handbook (7^th Edition):

Reich, Samuel. “To Affinity and Beyond: The Sound of Diatonic Positions.” 2020. Web. 11 Apr 2021.

Vancouver:

Reich S. To Affinity and Beyond: The Sound of Diatonic Positions. [Internet] [Doctoral dissertation]. University of Cincinnati; 2020. [cited 2021 Apr 11]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ucin1595849229880202.

Council of Science Editors:

Reich S. To Affinity and Beyond: The Sound of Diatonic Positions. [Doctoral Dissertation]. University of Cincinnati; 2020. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ucin1595849229880202

Texas State University – San Marcos

23. Nolen, Zachary Lee. A consideration into ways biology-based student organizations facilitate participation in STEM.

Degree: PhD, Aquatic Resources, 2019, Texas State University – San Marcos

URL: https://digital.library.txstate.edu/handle/10877/12883

► Student involvement on their campus is a vital part of their experience in higher education. A lack of involvement and engagement can lead to a… (more)

▼ Student involvement on their campus is a vital part of their experience in higher education. A lack of involvement and engagement can lead to a decreased sense of belonging on campus and in their chosen field of study. Researchers speculate that one reason students leave STEM is because students have not developed a strong perceived attachment to the field of science. One way that students may build this perceived attachment to science is through increasing their engagement and participation in science-based activities. By participating in science-based social organizations, students are exposed to new experiences they would not normally have during their coursework. The purpose of my study was to investigate how biology-based student organizations functioned as affinity groups and how these groups influenced individuals’ perceived cohesion to science. I followed three biology-based student organizations, biological honor society, microbiology club, and wildlife club, over the course of one academic year to identify the extent they exhibited the characteristics of affinity groups. After collecting and analyzing data from field observations, I found that all three groups exhibited the criteria of affinity groups to various degrees. Through analyzing student responses to an open-ended questionnaire, I was able to uncover the motivations students had for joining their respective student organizations and what benefits they reported receiving from their participation. I found three major overarching themes for what motivated students to join their respective student organization: they liked the content the organization was based on, to have some form of social outlet, or the reputation of the organization drew them into the organization. Students reported a wide range of benefits they received from their participation in these organizations. I grouped these benefits into five overarching themes: Networking, Professional Development, Learning Opportunities, Community Involvement, and Prestige. I found that there was some overlap between students’ motivations for joining their student organization and what benefit they received from their participation. With this overlap, I speculate that a feedback loop exists where students join an organization for a specific reason that guides what events they choose to participate in which then leads into the benefit they receive from their participation. Now that we better understand how these organizations function, what motivates students to join content-based student organizations, and what they are getting from their experiences, we can further promote these groups to new students. By joining a content-based student organization, students will be better able to find their place in science through networking with others in their field and honing and developing skills that they can take into the workforce, ultimately making them more competitive on the job market. Advisors/Committee Members: Daniel, Kristy (advisor), Bucklin, Carrie (committee member), Castro-Arellano, Ivan (committee member), Close, Eleanor (committee member), Williamson, Paula (committee member).

Subjects/Keywords: Student organizations; Affinity group; Sense of belonging

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nolen, Z. L. (2019). A consideration into ways biology-based student organizations facilitate participation in STEM. (Doctoral Dissertation). Texas State University – San Marcos. Retrieved from https://digital.library.txstate.edu/handle/10877/12883

Chicago Manual of Style (16^th Edition):

Nolen, Zachary Lee. “A consideration into ways biology-based student organizations facilitate participation in STEM.” 2019. Doctoral Dissertation, Texas State University – San Marcos. Accessed April 11, 2021. https://digital.library.txstate.edu/handle/10877/12883.

MLA Handbook (7^th Edition):

Nolen, Zachary Lee. “A consideration into ways biology-based student organizations facilitate participation in STEM.” 2019. Web. 11 Apr 2021.

Vancouver:

Nolen ZL. A consideration into ways biology-based student organizations facilitate participation in STEM. [Internet] [Doctoral dissertation]. Texas State University – San Marcos; 2019. [cited 2021 Apr 11]. Available from: https://digital.library.txstate.edu/handle/10877/12883.

Council of Science Editors:

Nolen ZL. A consideration into ways biology-based student organizations facilitate participation in STEM. [Doctoral Dissertation]. Texas State University – San Marcos; 2019. Available from: https://digital.library.txstate.edu/handle/10877/12883

Rutgers University

24. Butler, Kristie Lynn, 1989-. Role of Bcl-2 proteins in stress-induced apoptosis in a non-transformed mammary epithelial cell line.

Degree: MS, Microbiology and Molecular Genetics, 2014, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/44023/

► Apoptosis is the process of programmed cell death characterized by morphological and physiological changes executed by cells in response to a stimulus. The apoptotic process… (more)

▼ Apoptosis is the process of programmed cell death characterized by morphological and physiological changes executed by cells in response to a stimulus. The apoptotic process contributes to the loss of milk secreting cells (i.e. mammary epithelial cells) that occurs in the bovine mammary gland once peak lactation has occurred. However, the specific mechanisms by which this occurs are unknown. The goal of this work was to examine the role of the Bcl-2 family of proteins in regulating apoptosis in the bovine mammary epithelial cell line, MAC-T. The Bcl-2 family is divided into three categories based on protein function and combinations of four Bcl-2 homology (BH) domains. Anti-apoptotic multidomain proteins, such as Bcl-2 and Mcl-1, prevent apoptosis by binding to pro-apoptotic members of the family. Pro-apoptotic multidomain proteins, such as Bax, help to regulate apoptosis at the mitochondrial outer membrane. Pro-apoptotic BH3-only proteins, such as Bad and Bim, act as messengers between the anti-apoptotic and pro-apoptotic proteins. To investigate the roles of Bcl-2 proteins in apoptosis, MAC-T cells were treated with anisomycin (ANS), an activator of the intrinsic apoptotic pathway. ANS had little effect on Bcl-2 or Bax mRNA levels while it induced a 40 to 60% decrease in levels of Bad mRNA. Protein expression of Bax did not change during apoptosis, while Bcl-2, Mcl-1, and Bad expression decreased to varying degrees. The greatest change in protein levels was observed for Mcl-1, whose expression was nearly non-detectable after 4 h of treatment with ANS. Bim was phosphorylated in response to ANS but this was not caused by JNK or p38 signaling. Knock-down of Bim using siRNA decreased the ability of ANS to induce apoptosis. Mcl-1 and Bim protein expression changed in a similar time frame, therefore, interactions between the two proteins were evaluated using co-immunoprecipitation experiments. Mcl-1 and Bim interacted both basally and after treatment with ANS. The significance of Bim phosphorylation, the kinase responsible for its phosphorylation, and the functional significance of the interaction between Bim and Mcl-1 in stress-induced apoptosis remain to be determined. Advisors/Committee Members: Cohick, Wendie (chair), Belden, William (internal member), Di, Rong (internal member).

Subjects/Keywords: Apoptosis; Mammary glands – Secretions; Proteins – Affinity labeling

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APA (6^th Edition):

Butler, Kristie Lynn, 1. (2014). Role of Bcl-2 proteins in stress-induced apoptosis in a non-transformed mammary epithelial cell line. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/44023/

Chicago Manual of Style (16^th Edition):

Butler, Kristie Lynn, 1989-. “Role of Bcl-2 proteins in stress-induced apoptosis in a non-transformed mammary epithelial cell line.” 2014. Masters Thesis, Rutgers University. Accessed April 11, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/44023/.

MLA Handbook (7^th Edition):

Butler, Kristie Lynn, 1989-. “Role of Bcl-2 proteins in stress-induced apoptosis in a non-transformed mammary epithelial cell line.” 2014. Web. 11 Apr 2021.

Vancouver:

Butler, Kristie Lynn 1. Role of Bcl-2 proteins in stress-induced apoptosis in a non-transformed mammary epithelial cell line. [Internet] [Masters thesis]. Rutgers University; 2014. [cited 2021 Apr 11]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/44023/.

Council of Science Editors:

Butler, Kristie Lynn 1. Role of Bcl-2 proteins in stress-induced apoptosis in a non-transformed mammary epithelial cell line. [Masters Thesis]. Rutgers University; 2014. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/44023/

Uppsala University

25. Pelcman, Josef. Development of a method for kinetic characterisation of therapeutic antibodies in solution using the Gyrolab platform.

Degree: 2019, Uppsala University

URL: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-393505

► Therapeutic antibodies dominate the pharmaceutical market and improve the lives of millions of people annually. One important step when developing new medicines is to… (more)

Subjects/Keywords: kinetics; affinity; gyros; antibodies; antibody; kinetic; association; dissociation; affinity chromatography; Biophysics; Biofysik

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APA (6^th Edition):

Pelcman, J. (2019). Development of a method for kinetic characterisation of therapeutic antibodies in solution using the Gyrolab platform. (Thesis). Uppsala University. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-393505

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pelcman, Josef. “Development of a method for kinetic characterisation of therapeutic antibodies in solution using the Gyrolab platform.” 2019. Thesis, Uppsala University. Accessed April 11, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-393505.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pelcman, Josef. “Development of a method for kinetic characterisation of therapeutic antibodies in solution using the Gyrolab platform.” 2019. Web. 11 Apr 2021.

Vancouver:

Pelcman J. Development of a method for kinetic characterisation of therapeutic antibodies in solution using the Gyrolab platform. [Internet] [Thesis]. Uppsala University; 2019. [cited 2021 Apr 11]. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-393505.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pelcman J. Development of a method for kinetic characterisation of therapeutic antibodies in solution using the Gyrolab platform. [Thesis]. Uppsala University; 2019. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-393505

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Helsinki

26. Multia, Evgen. Interpreting the biosensor data of biomolecular interactions.

Degree: Department of Chemistry; Helsingfors universitet, Matematisk-naturvetenskapliga fakulteten, Kemiska institutionen, 2017, University of Helsinki

URL: http://hdl.handle.net/10138/229179

► The literature part of this thesis reviewed the process of obtaining affinity information with quartz crystal microbalance (QCM) and surface plasmon resonance (SPR) biosensors. Basic… (more)

▼ The literature part of this thesis reviewed the process of obtaining affinity information with quartz crystal microbalance (QCM) and surface plasmon resonance (SPR) biosensors. Basic principles of these biosensors were also evaluated, along with the principles of data acquisition and finally the data processing. The raw data produced by QCM or SPR can be used to study biomolecular interactions qualitatively and quantitatively. These techniques are also powerful in obtaining kinetic and thermodynamic information of the biomolecular interactions. SPR and QCM can produce data easily, but data interpretation can be sometimes problematic. This is partly due to misconceptions on how the sensograms should be interpreted. Many of the interpretational problems can and should be avoided long before the modeling of the data takes place to obtain reliable affinity data. The literature part of this thesis also presents tools for developing good experimental design. Well-designed experimental set-up is the most important element for producing good biosensor data. One should also estimate from the sensogram shapes what kind of analysis is needed. This was explained in detail in the literature part, pointing out the key elements how sensograms with certain shape should be interpreted and further analyzed to obtain affinity constants. Data analysis part of the literature review provides also information how to use appropriate models (e.g. fitting equilibrium, kinetic or complex data) with extensive examples. Surface site distribution model will be also covered as the tool to analyze complex biomolecular interactions by QCM and SPR. In the experimental part, affinity of anti-human apoB-100 monoclonal antibody (anti-apoB-100 Mab) towards different lipoproteins was studied with partially filling affinity capillary (PF-ACE) electrophoresis and QCM. PF-ACE with adsorption energy distribution (AED) calculations provided information on the heterogeneity of the interactions. For the first time, a modified surface site distribution model called Interaction map was utilized to model QCM data of lipoprotein interactions with anti-apoB-100 Mab. With the Interaction maps, it was possible to distinguish different kinetics of low-density lipoprotein (LDL) and anti-apoB-100 Mab interactions. Affinity constants obtained were used to evaluate thermodynamics of these interactions. Both methods were also used to evaluate interactions with other apoB-100 containing lipoproteins: intermediate-density lipoprotein (IDL) and very lowdensity lipoprotein (VLDL). It was found that the Interaction maps could distinguish two different kinetics from the mixture of IDL-VLDL with distinct affinity constants. Both methods agreed well with the affinity constants. It was found that the anti-apoB-100 Mab used in this study, had a high affinity towards apoB-100 containing lipoproteins. In the second part of the experimental, a convective interaction media (CIM) based LDL isolation platform was developed. In these studies, anti-apoB-100 Mab was immobilized on the…

Subjects/Keywords: kinetics; affinity; surface plasmon resonance; quartz crystal microbalance; affinity chromatography; antibody; capillary electrophoresis; binding studies; thermodynamics; partially filling affinity capillary electrophoresis; adsorption energy calculations; Analytical Chemistry; Analyyttinen kemia; Analytisk kemi

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APA (6^th Edition):

Multia, E. (2017). Interpreting the biosensor data of biomolecular interactions. (Masters Thesis). University of Helsinki. Retrieved from http://hdl.handle.net/10138/229179

Chicago Manual of Style (16^th Edition):

Multia, Evgen. “Interpreting the biosensor data of biomolecular interactions.” 2017. Masters Thesis, University of Helsinki. Accessed April 11, 2021. http://hdl.handle.net/10138/229179.

MLA Handbook (7^th Edition):

Multia, Evgen. “Interpreting the biosensor data of biomolecular interactions.” 2017. Web. 11 Apr 2021.

Vancouver:

Multia E. Interpreting the biosensor data of biomolecular interactions. [Internet] [Masters thesis]. University of Helsinki; 2017. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/10138/229179.

Council of Science Editors:

Multia E. Interpreting the biosensor data of biomolecular interactions. [Masters Thesis]. University of Helsinki; 2017. Available from: http://hdl.handle.net/10138/229179

Stellenbosch University

27. Liebenberg, Liesl Eileen. Non-covalent immobilisation of a ligand system : a new approach to affinity separation.

Degree: MSc, Biochemistry, 2003, Stellenbosch University

URL: http://hdl.handle.net/10019.1/53522

► ENGLISH ABSTRACT: Advances in pharmacology, biochemistry and biotechnology are increasingly dependant upon affinity chromatography as a preferred separation technique for the purification and characterisation of… (more)

▼ ENGLISH ABSTRACT: Advances in pharmacology, biochemistry and biotechnology are increasingly dependant upon affinity chromatography as a preferred separation technique for the purification and characterisation of specific biomolecules. In the past few years avidin-biotin technology has been widely and successfully used in the fields of medicine, pharmacy, biology and biochemistry. The avidin-biotin complex (ABC) has been used as a mediator for affinity chromatography, affinity cytochemistry, immunoassay, histopathology, bioaffinity sensors, erosslinking and immobilisation studies. The main reason for the popularity of the ABC and its growing usefulness in biotechnology is the exceptionally high affinity (1015 M-l) and stability of the noncovalent interaction between avidin and biotin. The use of the ABC is broadening as different biotin derivatives and avidin-containing conjugates are becoming commercially available. The aim of this work was to evaluate the usefulness of a plutonic" FI 08 and the ABC conjugate to effect affinity separation. Towards this aim, the adsorption of plutonic" F108 onto hydrophobic polysulphone membrane surfaces was studied. This information was used to determine the theoretical maximum amount of pluronic" FI08 that will adsorb onto a unit surface area of the membrane. It is known that the polypropylene oxide (PPO) centre block ofthe pluronic" F I08 surfactant molecule governs the concentration of pluronic" F I 08 molecules that will adsorb onto a given hydrophobic surface. If the maximum coating concentration of plutonic" FI08 is known, one can assume that the maximum coating concentration of any pluronic derivative, with the same PPO centre block size, will be the same. Adsorption studies were carried out, the Langmuir adsorption isotherm was determined, and subsequently the fractional coating was calculated. The end-groups of plutonic" FI08 were modified as follows and the substituted pluronic was adsorbed onto a membrane that was to act as the solid support matrix in the development of an affinity system: Amino pluronic was synthesised by first tosylating pluronic" FI08, followed by azidation with NaN3 then reduction with LiAI~. The synthesised amino pluronic was then biotinylated using N-hydroxysuccinimide biotin ester. The suitability of this synthetic route was first assessed on a model compound, 2-methoxyethylamine, and validated by NMR (Nuclear Magnetic Resonance) spectroscopy. The synthetic protocol was then used to derivatise the larger pluronic molecule. The affinity system was tested on two different hydrophobic surfaces: polystyrene and polysulphone membranes (PSMs). Avidin-conjugated horseradish peroxidase was obtained and used to interact with the immobilised biotin. The enzymatic reaction of the coupled peroxidase converted the substrate, 2, 2'-azino-di-(3-ethyl-benzthiazoline-6-sulphonic acid) (ABTS) to a coloured product. The colour developed is proportional to the amount of biotin that was immobilised on the hydrophobic surfaces studied. … Advisors/Committee Members: Swart, P., Jacobs, E. P., Bredenkamp, M. W., Stellenbosch University. Faculty of Science. Dept. of Biochemistry..

Subjects/Keywords: Affinity chromatography; Chemical affinity; Avidin; Coordination compounds; Ligands (Biochemistry)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Liebenberg, L. E. (2003). Non-covalent immobilisation of a ligand system : a new approach to affinity separation. (Masters Thesis). Stellenbosch University. Retrieved from http://hdl.handle.net/10019.1/53522

Chicago Manual of Style (16^th Edition):

Liebenberg, Liesl Eileen. “Non-covalent immobilisation of a ligand system : a new approach to affinity separation.” 2003. Masters Thesis, Stellenbosch University. Accessed April 11, 2021. http://hdl.handle.net/10019.1/53522.

MLA Handbook (7^th Edition):

Liebenberg, Liesl Eileen. “Non-covalent immobilisation of a ligand system : a new approach to affinity separation.” 2003. Web. 11 Apr 2021.

Vancouver:

Liebenberg LE. Non-covalent immobilisation of a ligand system : a new approach to affinity separation. [Internet] [Masters thesis]. Stellenbosch University; 2003. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/10019.1/53522.

Council of Science Editors:

Liebenberg LE. Non-covalent immobilisation of a ligand system : a new approach to affinity separation. [Masters Thesis]. Stellenbosch University; 2003. Available from: http://hdl.handle.net/10019.1/53522

NSYSU

28. Ding, Sheng-che. The contribution of non-native structure with recombinant cobrotoxin to its immunoreactivity toward anti-cobrotoxin antibodies.

Degree: Master, Institute of Biomedical Sciences, 2009, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0630109-170406

► To induce the production of antibodies, exogenous antigens are taken up and degraded in antigen presenting cells in vivo. Since this process inevitably lead to… (more)

Subjects/Keywords: Anti-cobrotoxin antibodies; ELISA; Conformation-dependent; Cobrotoxin; binding affinity

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APA (6^th Edition):

Ding, S. (2009). The contribution of non-native structure with recombinant cobrotoxin to its immunoreactivity toward anti-cobrotoxin antibodies. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0630109-170406

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ding, Sheng-che. “The contribution of non-native structure with recombinant cobrotoxin to its immunoreactivity toward anti-cobrotoxin antibodies.” 2009. Thesis, NSYSU. Accessed April 11, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0630109-170406.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ding, Sheng-che. “The contribution of non-native structure with recombinant cobrotoxin to its immunoreactivity toward anti-cobrotoxin antibodies.” 2009. Web. 11 Apr 2021.

Vancouver:

Ding S. The contribution of non-native structure with recombinant cobrotoxin to its immunoreactivity toward anti-cobrotoxin antibodies. [Internet] [Thesis]. NSYSU; 2009. [cited 2021 Apr 11]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0630109-170406.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ding S. The contribution of non-native structure with recombinant cobrotoxin to its immunoreactivity toward anti-cobrotoxin antibodies. [Thesis]. NSYSU; 2009. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0630109-170406

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

29. YEH, SHU-WEN. Sequence effects on the proton-transfer reaction of the guanine-cytosine base pair radical anion and cation.

Degree: PhD, Chemistry, 2012, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0716112-205715

► The formation of base pair radical anions and cations is closely related to many fascinating research fields in biology and chemistry such as genetic mutation,… (more)

▼ The formation of base pair radical anions and cations is closely related to many fascinating research fields in biology and chemistry such as genetic mutation, radiation-induced DNA damage and dynamics of charge transfer in DNA. However, the relevant knowledge so far mainly comes from studies on isolated base pair radical anions and cations, and their behavior in the DNA environment is less understood. In this study, we focus on how the nucleobase sequence affects the properties of the guanineâcytosine (G:C) base pair radical anion and cation. The energetic barrier and reaction energy for the proton transfer along the N1(G)âHâ¢â¢â¢N3(C) hydrogen bond and the stability of (G:C)â¢ (i.e., electron affinity and ionization potential of G:C) embedded in different sequences of base-pair trimer were evaluated using density functional theory and two-layer ONIOM method. The computational results demonstrated that the presence of neighboring base pairs has an important influence on the behavior of (G:C)â¢ in the gas phase. The excess electron and positive hole were found to be localized on the embedded G:C and the charge leakage to neighboring base pairs was very minor in all of the investigated sequences. Accordingly, the sequence behavior of the proton transfer reaction and the stability of (G:C)â¢ is chiefly governed by electrostatic interactions with adjacent base pairs. However, the effect of base stacking, due to its electrostatic nature, is severely screened upon hydration, and thus, the sequence dependence of the properties of (G:C)â¢ in aqueous environment becomes relatively weak and less than that observed in the gas phase. The effect of geometry relaxation associated with neighboring base pairs as well as the possibility of proton transfer along the N2(G)âHâ¢â¢â¢O2(C) channel have also been investigated. The implications of the present findings to the electron transport and radiation damage of DNA are discussed. Advisors/Committee Members: Po-Yu Chen (chair), Chin-Hsing Chou (chair), Chai-Lin Kao (chair), Hsing-Yin Chen (committee member), Chih-Neng Hsu (chair), Teng-Yuan Dong (committee member).

Subjects/Keywords: electron affinity; ionization potential; cytosine; DFT; guanine; proton transfer reaction

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

YEH, S. (2012). Sequence effects on the proton-transfer reaction of the guanine-cytosine base pair radical anion and cation. (Doctoral Dissertation). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0716112-205715

Chicago Manual of Style (16^th Edition):

YEH, SHU-WEN. “Sequence effects on the proton-transfer reaction of the guanine-cytosine base pair radical anion and cation.” 2012. Doctoral Dissertation, NSYSU. Accessed April 11, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0716112-205715.

MLA Handbook (7^th Edition):

YEH, SHU-WEN. “Sequence effects on the proton-transfer reaction of the guanine-cytosine base pair radical anion and cation.” 2012. Web. 11 Apr 2021.

Vancouver:

YEH S. Sequence effects on the proton-transfer reaction of the guanine-cytosine base pair radical anion and cation. [Internet] [Doctoral dissertation]. NSYSU; 2012. [cited 2021 Apr 11]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0716112-205715.

Council of Science Editors:

YEH S. Sequence effects on the proton-transfer reaction of the guanine-cytosine base pair radical anion and cation. [Doctoral Dissertation]. NSYSU; 2012. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0716112-205715

University of Pretoria

30. [No author]. Employer brand identification influence on total reward structure .

Degree: 2011, University of Pretoria

URL: http://upetd.up.ac.za/thesis/available/etd-07172011-153625/

► Companies compete for employees based on their perceived employer brand value. This study investigates what influences a strong or weak employer brand has on the… (more)

Subjects/Keywords: UCTD; Total rewards; Employer brand; Brand theory; Brand affinity

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

author], [. (2011). Employer brand identification influence on total reward structure . (Masters Thesis). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-07172011-153625/

Chicago Manual of Style (16^th Edition):

author], [No. “Employer brand identification influence on total reward structure .” 2011. Masters Thesis, University of Pretoria. Accessed April 11, 2021. http://upetd.up.ac.za/thesis/available/etd-07172011-153625/.

MLA Handbook (7^th Edition):

author], [No. “Employer brand identification influence on total reward structure .” 2011. Web. 11 Apr 2021.

Vancouver:

author] [. Employer brand identification influence on total reward structure . [Internet] [Masters thesis]. University of Pretoria; 2011. [cited 2021 Apr 11]. Available from: http://upetd.up.ac.za/thesis/available/etd-07172011-153625/.

Council of Science Editors:

author] [. Employer brand identification influence on total reward structure . [Masters Thesis]. University of Pretoria; 2011. Available from: http://upetd.up.ac.za/thesis/available/etd-07172011-153625/

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Open Access Theses and Dissertations