subject:(Adhesions)

University of Alberta

1. Maciver, Allison H. Development of a Sirolimus-eluting Mesh to Reduce Intra-Abdominal Adhesions.

Degree: MS, Department of Surgery, 2012, University of Alberta

URL: https://era.library.ualberta.ca/files/h702q7990

► Postoperative intra-abdominal adhesions are a significant clinical problem. This project addresses the potential novel role for a sirolimus-eluting hydrogel in the setting of postoperative adhesions… (more)

Subjects/Keywords: sirolimus; surgery; adhesions

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APA (6^th Edition):

Maciver, A. H. (2012). Development of a Sirolimus-eluting Mesh to Reduce Intra-Abdominal Adhesions. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/h702q7990

Chicago Manual of Style (16^th Edition):

Maciver, Allison H. “Development of a Sirolimus-eluting Mesh to Reduce Intra-Abdominal Adhesions.” 2012. Masters Thesis, University of Alberta. Accessed January 15, 2021. https://era.library.ualberta.ca/files/h702q7990.

MLA Handbook (7^th Edition):

Maciver, Allison H. “Development of a Sirolimus-eluting Mesh to Reduce Intra-Abdominal Adhesions.” 2012. Web. 15 Jan 2021.

Vancouver:

Maciver AH. Development of a Sirolimus-eluting Mesh to Reduce Intra-Abdominal Adhesions. [Internet] [Masters thesis]. University of Alberta; 2012. [cited 2021 Jan 15]. Available from: https://era.library.ualberta.ca/files/h702q7990.

Council of Science Editors:

Maciver AH. Development of a Sirolimus-eluting Mesh to Reduce Intra-Abdominal Adhesions. [Masters Thesis]. University of Alberta; 2012. Available from: https://era.library.ualberta.ca/files/h702q7990

University of Manchester

2. Berry, James. The role of visceral peritoneum in LPS mediated inflammatory responses.

Degree: Thesis (M.D.), 2017, University of Manchester

URL: https://www.research.manchester.ac.uk/portal/en/theses/the-role-of-visceral-peritoneum-in-lps-mediated-inflammatory-responses(f09bc8e8-b2e2-4648-9a0c-3bbbfff5c295).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.719318

► Peritoneal adhesions pose a considerable postoperative burden. The mechanisms underlying adhesion formation remain poorly understood but relate principally to inflammation and fibrinolysis. The peritoneum may… (more)

▼ Peritoneal adhesions pose a considerable postoperative burden. The mechanisms underlying adhesion formation remain poorly understood but relate principally to inflammation and fibrinolysis. The peritoneum may coordinate the inflammatory response that leads to adhesion formation but human adhesion formation cannot be studied directly so most research has employed animal models and individual transformed cell lines. Furthermore, the specific roles of the visceral or parietal peritoneum in adhesion formation are unclear. The aims of the studies undertaken in this thesis are to investigate the regulation of a gut bacteria-driven inflammatory response in human visceral peritoneum. A novel ex vivo model was designed using human small bowel visceral peritoneum, cultured with E. coli derived LPS, for 18 to 48 hours. ELISA and rtPCR were used to analyse supernatant protein concentration and gene expression respectively for key inflammatory mediators associated with adhesion formation including cytokines (IL-6, IL-10, TNFalpha), transforming growth factor-β1 (TGF-β1) and fibrinolytic factors (tPA and PAI-1) as well as the signalling receptors thought to be involved in LPS-mediated inflammation (TLR2 and 4). LPS exposure resulted in stimulation of IL-6, IL-10 and TNFalpha production from human visceral peritoneum at 18 hours. These increases correlated with increased gene expression for IL-6 and TNFalpha but not IL-10. TGF-β1 production and gene expression were unchanged at 18 & 24 hours but increased at 48 hours when co-incubated with IL-1β. PAI-1 was increased but tPA was unchanged following LPS stimulation, suggesting a reduction in peritoneal fibrinolytic activity. Peritoneal tissue expressed the signalling receptors TLR 2 and 4. However, inhibition of TLR 2 or 4 failed to abrogate production of cytokines in cultured primary mesothelial cells or peritoneal tissue post-LPS exposure. TLR 4 inhibition did reduce mesothelial cell production of chemokines, CCL2 and CXCL1. These novel findings demonstrated that human visceral peritoneum actively participates in LPS-induced peritoneal inflammatory responses and reduced fibrinolysis which may account for the visceral peritoneum's affinity to form adhesions. Ex vivo human peritoneal culture is a novel, feasible and reproducible method of investigating the role of the peritoneum in inflammation.

Subjects/Keywords: 616.6; Peritoneal inflammation; Adhesions

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APA (6^th Edition):

Berry, J. (2017). The role of visceral peritoneum in LPS mediated inflammatory responses. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/the-role-of-visceral-peritoneum-in-lps-mediated-inflammatory-responses(f09bc8e8-b2e2-4648-9a0c-3bbbfff5c295).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.719318

Chicago Manual of Style (16^th Edition):

Berry, James. “The role of visceral peritoneum in LPS mediated inflammatory responses.” 2017. Doctoral Dissertation, University of Manchester. Accessed January 15, 2021. https://www.research.manchester.ac.uk/portal/en/theses/the-role-of-visceral-peritoneum-in-lps-mediated-inflammatory-responses(f09bc8e8-b2e2-4648-9a0c-3bbbfff5c295).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.719318.

MLA Handbook (7^th Edition):

Berry, James. “The role of visceral peritoneum in LPS mediated inflammatory responses.” 2017. Web. 15 Jan 2021.

Vancouver:

Berry J. The role of visceral peritoneum in LPS mediated inflammatory responses. [Internet] [Doctoral dissertation]. University of Manchester; 2017. [cited 2021 Jan 15]. Available from: https://www.research.manchester.ac.uk/portal/en/theses/the-role-of-visceral-peritoneum-in-lps-mediated-inflammatory-responses(f09bc8e8-b2e2-4648-9a0c-3bbbfff5c295).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.719318.

Council of Science Editors:

Berry J. The role of visceral peritoneum in LPS mediated inflammatory responses. [Doctoral Dissertation]. University of Manchester; 2017. Available from: https://www.research.manchester.ac.uk/portal/en/theses/the-role-of-visceral-peritoneum-in-lps-mediated-inflammatory-responses(f09bc8e8-b2e2-4648-9a0c-3bbbfff5c295).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.719318

University of Texas – Austin

3. Mayes, Sarah Margaret. Hyaluronic acid and alginate blend hydrogel films for the prevention of postsurgical adhesions.

Degree: PhD, Biomedical Engineering, 2013, University of Texas – Austin

URL: http://hdl.handle.net/2152/22058

► Postoperative adhesions form as the body's natural response to injury in an effort to temporarily protect and supply nutrients to these tissues. However, adhesions can… (more)

▼ Postoperative adhesions form as the body's natural response to injury in an effort to temporarily protect and supply nutrients to these tissues. However, adhesions can remain permanent, and fail otherwise successful surgeries by tethering tissues together that are normally separated. An ideal anti-adhesive device reduces unwanted adhesions and leaves the patient in a state most similar to before surgery. This dissertation introduces a novel, robust hydrogel film consisting of two hydrophilic polydisaccharides, hyaluronic acid (HA) and alginate. To address the challenge of retaining HA in alginate-rich hydrogels, we methacrylated the HA (GMHA), creating hydrophobic moieties. These hydrophobic interactions shift the percolation threshold, allowing for greater concentrations of GMHA to be retained in resulting films. UV crosslinking retains GMHA beyond the percolation threshold and widens the possibilities of usable films. To enhance the mechanical properties of these alginate/GMHA films, we employed a previously developed method for creating thin, branched, interconnected fibers using urea crystal templating. Templated films are softer and, yet, tougher than films that have not been templated. This toughness is a result of increased density of polymer in the fibers. These films were selected as most conformable and most robust by surgeons in a blinded handling study. In a rat peritoneal abrasion model for adhesion formation, the films successfully prevented adhesions with statistical equivalence to the leading anti-adhesion device commercially available. Finally, future recommendations are suggested for the development of a bilayer construct with a collagen/alginate blend bound to an alginate/HA layer for an anti-adhesive and regenerative strategy. This construct addresses the need for opposing strategies in the dynamic environment of wound healing. Further research is needed to develop the usefulness of this bilayer system, as preventing unwanted adhesions is merely a first step in achieving a blemish-free healed wound. Advisors/Committee Members: Stachowiak, Jeanne Casstevens (advisor), Schmidt, Christine E. (advisor).

Subjects/Keywords: Hyaluronic acid; Alginate; Abdominal adhesions

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APA (6^th Edition):

Mayes, S. M. (2013). Hyaluronic acid and alginate blend hydrogel films for the prevention of postsurgical adhesions. (Doctoral Dissertation). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/22058

Chicago Manual of Style (16^th Edition):

Mayes, Sarah Margaret. “Hyaluronic acid and alginate blend hydrogel films for the prevention of postsurgical adhesions.” 2013. Doctoral Dissertation, University of Texas – Austin. Accessed January 15, 2021. http://hdl.handle.net/2152/22058.

MLA Handbook (7^th Edition):

Mayes, Sarah Margaret. “Hyaluronic acid and alginate blend hydrogel films for the prevention of postsurgical adhesions.” 2013. Web. 15 Jan 2021.

Vancouver:

Mayes SM. Hyaluronic acid and alginate blend hydrogel films for the prevention of postsurgical adhesions. [Internet] [Doctoral dissertation]. University of Texas – Austin; 2013. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/2152/22058.

Council of Science Editors:

Mayes SM. Hyaluronic acid and alginate blend hydrogel films for the prevention of postsurgical adhesions. [Doctoral Dissertation]. University of Texas – Austin; 2013. Available from: http://hdl.handle.net/2152/22058

University of Manchester

4. Balloi, Eleonora. Investigating Conformational Changes of Proteins Using Fӧrster Resonance Energy Transfer.

Degree: 2015, University of Manchester

URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:259362

► Fӧrster Resonance Energy Transfer (FRET)-based techniques are gaining an increasing importance in cell biology and cell-matrix adhesion studies because they allow both the detection of… (more)

▼ Fӧrster Resonance Energy Transfer (FRET)-based techniques are gaining an increasing importance in cell biology and cell-matrix adhesion studies because they allow both the detection of conformational changes of target proteins and their localisation in cells. Frequency Domain-Fluorescence Lifetime Microscopy (FD-FLIM) is currently considered one of the most reliable methods to measure FRET in live cells. However, due to its dependence on many technical prerequisites, its use is not yet widespread. The purpose of this work was to first establish FD-FLIM measurements of FRET on a new FD-FLIM microscope module. Then we aimed to apply FD-FLIM-FRET measurements to the study of conformational changes of the cell matrix-adhesion proteins vinculin and integrin and of the growth factor receptor Tie-2. In the first part of the work, published FRET probes including distance-sensors and two sets of vinculin-based probes were extensively tested with FD-FLIM, sensitised emission and ratiometric FRET. FD-FLIM was shown to be the most accurate method in approximating molecular distances between fluorophores. Moreover this study unveiled specific caveats associated with both existing vinculin FRET probes. FD-FLIM was then used to study conformational changes of the extracellular matrix receptor αvβ3 integrin and of the angiopoietin receptor Tie-2 using specific FRET probes designed by us. While data showed that the αv-integrin-FRET probe localised to adhesion sites, more experiments will be required to evaluate its full functionality. The Tie-2-FRET probe was fully functional and, upon ligand binding, allowed the detection of a bending movement of the extracellular domain towards the cell membrane.Finally, a combination of FRET, immunofluorescence and tension release experiments were used to show that intracellular tension is not required to maintain integrins in their activated conformation. However, intracellular tension is required to recruit other key proteins such as vinculin, talin and tensin to adhesions sites. Overall this work demonstrates the importance of FD-FLIM-FRET as a tool to investigate conformational changes of adhesion proteins and transmembrane receptors within the cell environment. Advisors/Committee Members: HUMPHRIES, MARTIN MJ, Humphries, Martin, Ballestrem, Christoph.

Subjects/Keywords: focal adhesions; fibrillar adhesions; integrin; vinculin; Tie-2; FRET; FD-FLIM

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APA (6^th Edition):

Balloi, E. (2015). Investigating Conformational Changes of Proteins Using Fӧrster Resonance Energy Transfer. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:259362

Chicago Manual of Style (16^th Edition):

Balloi, Eleonora. “Investigating Conformational Changes of Proteins Using Fӧrster Resonance Energy Transfer.” 2015. Doctoral Dissertation, University of Manchester. Accessed January 15, 2021. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:259362.

MLA Handbook (7^th Edition):

Balloi, Eleonora. “Investigating Conformational Changes of Proteins Using Fӧrster Resonance Energy Transfer.” 2015. Web. 15 Jan 2021.

Vancouver:

Balloi E. Investigating Conformational Changes of Proteins Using Fӧrster Resonance Energy Transfer. [Internet] [Doctoral dissertation]. University of Manchester; 2015. [cited 2021 Jan 15]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:259362.

Council of Science Editors:

Balloi E. Investigating Conformational Changes of Proteins Using Fӧrster Resonance Energy Transfer. [Doctoral Dissertation]. University of Manchester; 2015. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:259362

Georgia Tech

5. Zhou, Dennis Wei. Force-signaling coupling at single focal adhesions.

Degree: PhD, Biomedical Engineering (Joint GT/Emory Department), 2019, Georgia Tech

URL: http://hdl.handle.net/1853/62651

► Integrin-mediated adhesion to extracellular matrices (ECM) provides forces and signals that direct cell processes central to tissue organization, homeostasis, and disease. Recent studies show an… (more)

▼ Integrin-mediated adhesion to extracellular matrices (ECM) provides forces and signals that direct cell processes central to tissue organization, homeostasis, and disease. Recent studies show an important relationship between cell adhesive force generation and focal adhesion (FA) assembly, yet it remains unclear how forces are transduced into adhesive signals. Our work seeks to identify coupling between cell adhesive force generation and signaling at FAs. To measure forces, we used Microfabricated Post-Array-Deflectors (mPADs), which are an array of PDMS ~1.8 µm diameter microposts. Based on the micropost deflections, we can calculate the forces exerted by cells. We previously showed that vinculin regulates force transmission at FAs. Vinculin residence time in FAs correlated with applied force, supporting a mechanosensitive model in which forces stabilize vinculin’s active conformation to promote force transfer. We first examined the relationship between traction force and vinculin-paxillin localization to single FAs in the context of substrate stiffness and actomyosin contractility. Substrate stiffness and contractility regulated vinculin localization to FAs, and vinculin auto-inhibition is a crucial regulatory step in this process that overrides the effects of cytoskeletal tension and substrate stiffness. Vinculin and paxillin FA area did not correlate with traction force magnitudes at single FAs, and this was consistent across different ECM stiffness and cytoskeletal tension states. Vinculin residence time at FAs linearly varied with applied force for stiff substrates, but this coupling was disrupted on soft substrates and in the presence of contractility inhibitors. In contrast, paxillin residence time at FAs was independent of force, substrate stiffness, and cytoskeletal contractility. Lastly, substrate stiffness and cytoskeletal contractility regulated whether vinculin and paxillin turnover dynamics are correlated to each other at single FAs. We also found that pFAK Y397 levels are linearly coupled to force at single FAs on stiff substrates. On soft substrates, however, this positive relationship is eliminated. We found that talin is required for FAK localization and Y397 phosphorylation at FAs and mediates force-FAK linear coupling at FAs via talin-FAK binding. Furthermore, averaged levels of FAK localization and Y397 phosphorylation at FAs are relatively insensitive to vinculin expression. However, a full-length vinculin molecule that binds talin and actin is required for linear coupling to occur between force-FAK localization and force-FAK Y397 phosphorylation at individual FAs. Lastly, we demonstrate that a full-length vinculin molecule that binds talin and actin is required to promote YAP nuclear accumulation. These findings suggest that force generation and signaling are coupled at FAs and underscore the role of environmental stiffness, talin, and vinculin in regulating force-signaling coupling at FAs. Our results generate new insights into how cell adhesive forces are integrated into biochemical signals.… Advisors/Committee Members: Garcia, Andres J. (advisor), Zhu, Cheng (committee member), Curtis, Jennifer E. (committee member), Kowalczyk, Andrew P. (committee member), del Campo, Aranzazu (committee member).

Subjects/Keywords: Focal adhesions; Mechanobiology; FAK; Mechanotransduction; Vinculin; YAP

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zhou, D. W. (2019). Force-signaling coupling at single focal adhesions. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/62651

Chicago Manual of Style (16^th Edition):

Zhou, Dennis Wei. “Force-signaling coupling at single focal adhesions.” 2019. Doctoral Dissertation, Georgia Tech. Accessed January 15, 2021. http://hdl.handle.net/1853/62651.

MLA Handbook (7^th Edition):

Zhou, Dennis Wei. “Force-signaling coupling at single focal adhesions.” 2019. Web. 15 Jan 2021.

Vancouver:

Zhou DW. Force-signaling coupling at single focal adhesions. [Internet] [Doctoral dissertation]. Georgia Tech; 2019. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/1853/62651.

Council of Science Editors:

Zhou DW. Force-signaling coupling at single focal adhesions. [Doctoral Dissertation]. Georgia Tech; 2019. Available from: http://hdl.handle.net/1853/62651

Penn State University

6. Moroney, George J. Characterization of Early Membrane Adhesion Activity with a Drift-corrected Optical Trap.

Degree: 2015, Penn State University

URL: https://submit-etda.libraries.psu.edu/catalog/25065

► Vascular endothelial cells have been shown to sense the pressure and shear stress imparted by blood flow and to convert these mechanical stimuli into intracellular… (more)

▼ Vascular endothelial cells have been shown to sense the pressure and shear stress imparted by blood flow and to convert these mechanical stimuli into intracellular and extracellular chemical products such as prostacyclin and nitric oxide. Such products alter cardiovascular function by their ability to adjust vascular dilation and inhibit platelet aggregation and clotting. A leading hypothesis for mechanotransduction suggests that forces alter the interaction of integrin-based adhesions to extracellular matrix molecules. Such interactions are expected to be on the time scale of cardiac pulsatility (1-2 Hz). Thus, a greater understanding of the precise magnitude and temporal characteristics of cell adhesion would be instrumental in understanding the fundamental mechanisms of mechanotransduction. Whereas the cell interacts with its environment via the assembly of membrane proteins to form focal adhesions, studies on the early dynamics of force production by optical trapping would lead to a fundamental understanding of mechanotransduction by vascular endothelium. This thesis describes the development, testing, and optimization of protocols and instrumentation for the characterization of integrin-based adhesion to extracellular matrix proteins. Optical trapping allows for application of forces to single molecules and molecular clusters of similar magnitude (~2 pN) to the external forces that these molecules experience in vivo. More importantly for this study, the optical trap is able to detect the exact time that a force is applied to the cell. In order to monitor the activity of adhesion events, a continuous focus drift mechanism with a 100 nanometer-scale sensitivity was developed. This technique measures the changes in distance between the objective and cover slip by measuring the intensity of the light from the epi pathway of the microscope that reflects off of the cover slip. The early adhesion dynamics of the cells were studies using both a variance-based and time-to-adhesion-based assay using fibronectin-functionalized beads in contact with the membrane of human aortic endothelial cells. Preliminary results suggest that decreasing membrane fluidity with benzyl alcohol decreases time to adhesion, suggesting that a principle mechanism of force transduction depends on lateral transport of integrins to the adhesion site. Advisors/Committee Members: Peter J Butler, Thesis Advisor/Co-Advisor.

Subjects/Keywords: Focal adhesions; mechanotransduction; optical trap; drift correction

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APA (6^th Edition):

Moroney, G. J. (2015). Characterization of Early Membrane Adhesion Activity with a Drift-corrected Optical Trap. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/25065

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Moroney, George J. “Characterization of Early Membrane Adhesion Activity with a Drift-corrected Optical Trap.” 2015. Thesis, Penn State University. Accessed January 15, 2021. https://submit-etda.libraries.psu.edu/catalog/25065.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Moroney, George J. “Characterization of Early Membrane Adhesion Activity with a Drift-corrected Optical Trap.” 2015. Web. 15 Jan 2021.

Vancouver:

Moroney GJ. Characterization of Early Membrane Adhesion Activity with a Drift-corrected Optical Trap. [Internet] [Thesis]. Penn State University; 2015. [cited 2021 Jan 15]. Available from: https://submit-etda.libraries.psu.edu/catalog/25065.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Moroney GJ. Characterization of Early Membrane Adhesion Activity with a Drift-corrected Optical Trap. [Thesis]. Penn State University; 2015. Available from: https://submit-etda.libraries.psu.edu/catalog/25065

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – San Diego

7. Burdick, Austin. Biocompatibility and Delivery of a Hydrogel Barrier for the Prevention of Postsurgical Cardiac Adhesions.

Degree: Bioengineering, 2018, University of California – San Diego

URL: http://www.escholarship.org/uc/item/7f6839hw

► Previous research into the problem of postsurgical cardiac adhesions has produced a hydrogel barrier that can be applied to the surface of the heart, preventing… (more)

Subjects/Keywords: Bioengineering; Adhesions; Cardiovascular; Fluid Dynamics; Hydrogel

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APA (6^th Edition):

Burdick, A. (2018). Biocompatibility and Delivery of a Hydrogel Barrier for the Prevention of Postsurgical Cardiac Adhesions. (Thesis). University of California – San Diego. Retrieved from http://www.escholarship.org/uc/item/7f6839hw

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Burdick, Austin. “Biocompatibility and Delivery of a Hydrogel Barrier for the Prevention of Postsurgical Cardiac Adhesions.” 2018. Thesis, University of California – San Diego. Accessed January 15, 2021. http://www.escholarship.org/uc/item/7f6839hw.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Burdick, Austin. “Biocompatibility and Delivery of a Hydrogel Barrier for the Prevention of Postsurgical Cardiac Adhesions.” 2018. Web. 15 Jan 2021.

Vancouver:

Burdick A. Biocompatibility and Delivery of a Hydrogel Barrier for the Prevention of Postsurgical Cardiac Adhesions. [Internet] [Thesis]. University of California – San Diego; 2018. [cited 2021 Jan 15]. Available from: http://www.escholarship.org/uc/item/7f6839hw.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Burdick A. Biocompatibility and Delivery of a Hydrogel Barrier for the Prevention of Postsurgical Cardiac Adhesions. [Thesis]. University of California – San Diego; 2018. Available from: http://www.escholarship.org/uc/item/7f6839hw

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

8. Lauder, Christopher Ian William. Peritoneal adhesion formation and modulation.

Degree: 2011, University of Adelaide

URL: http://hdl.handle.net/2440/69662

► This thesis examines the subject of peritoneal adhesion formation following surgery in the format of a Master of Surgery by publication. A comprehensive literature review… (more)

▼ This thesis examines the subject of peritoneal adhesion formation following surgery in the format of a Master of Surgery by publication. A comprehensive literature review examines all aspects of peritoneal adhesions from the basic science to the evidence supporting products aimed at adhesion reduction. Subsequent experimental work utilises two animal models to form adhesions and test the ability of a novel gel product to reduce adhesion formation. The gel is a hybrid hydrogel consisting of modified chitosan and dextran. These two components are combined by a chemical cross linking reaction to form an inert gel that can be applied to the site of surgery. The gel confers several beneficial properties when used to prevent adhesions. Firstly it provides a physical separation of the injured peritoneal surfaces whilst also inhibiting the ingress of fibroblasts to the area. Secondary characteristics which promote haemostasis and inhibit bacterial growth enhance the gels adhesion reducing potential. Initially the gel was trialled in a small animal model to test varying compositions and volumes of the gel. Two different surgical models of adhesion formation were utilised to provide a range of stimuli in the post operative period. Results from these experiments were encouraging, showing a statistically significant reduction in adhesion formation. Following on from this initial study a large animal study was conceived to further evaluate the effectiveness of the gel in differing environments. The porcine model also allowed for a true bowel resection with anastomosis to test the safety of the gel when used in this scenario. Allied to this the gel was also trialled following adhesiolysis at the mid point of the study, while monitoring for sensitisation or toxicity to the gel. Infective complications and abscess formation proved to be a difficult hurdle to overcome in this model. As such, no significant reduction in adhesion reformation following adhesiolysis was observed. There were however some promising results with a reduction in adhesions to the wound noted with treatment at the time of laparoscopy, as well as a reduction in adhesions involving the bowel at the study end point. The experimental work highlights the difficulties associated with peritoneal adhesion reduction. Overcoming the numerous stimuli to adhesion formation is not an easy task, and there remains no currently available treatment for the prevention of adhesions without certain caveats to its use. An effective product that could be used safely in practically all environments would certainly be a step forward in this branch of surgical research. It is plausible that a product such as the gel may be improved upon to show further benefit. However, long term studies will still be required to show a beneficial effect in long term outcome measures such as the incidence of small bowel obstruction. Advisors/Committee Members: Maddern, Guy John (advisor), School of Medicine (school).

Subjects/Keywords: peritoneal adhesions; adhesion prevention; adhesion treatment

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APA (6^th Edition):

Lauder, C. I. W. (2011). Peritoneal adhesion formation and modulation. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/69662

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lauder, Christopher Ian William. “Peritoneal adhesion formation and modulation.” 2011. Thesis, University of Adelaide. Accessed January 15, 2021. http://hdl.handle.net/2440/69662.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lauder, Christopher Ian William. “Peritoneal adhesion formation and modulation.” 2011. Web. 15 Jan 2021.

Vancouver:

Lauder CIW. Peritoneal adhesion formation and modulation. [Internet] [Thesis]. University of Adelaide; 2011. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/2440/69662.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lauder CIW. Peritoneal adhesion formation and modulation. [Thesis]. University of Adelaide; 2011. Available from: http://hdl.handle.net/2440/69662

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Columbia University

9. Saxena, Mayur. Mechanisms of Focal Adhesions.

Degree: 2018, Columbia University

URL: https://doi.org/10.7916/D8NZ9R3F

► Focal adhesions are dynamic multiprotein structures connecting cells to their surrounding microenvironment. Cells receive critical mechanical signals from adhesions that control many cellular processes including… (more)

▼ Focal adhesions are dynamic multiprotein structures connecting cells to their surrounding microenvironment. Cells receive critical mechanical signals from adhesions that control many cellular processes including wound healing, differentiation, development, and cancer. Proteins that form adhesions are called adhesion proteins and some of these proteins can be mechanosensitive, meaning that they respond to mechanical stimuli. During spreading and migration, cells mechanically test extracellular matrix rigidity by contracting matrix to a constant displacement. Transmission and processing of such mechanical signals rely upon the dynamic regulation of the adhesions, which is tightly coordinated with activation of intracellular signaling cascades involving various adhesion molecules. However, the molecular mechanisms of mechanical signals that are transmitted through the adhesions to control cell behavior are poorly understood. In this thesis, we discovered novel phenomenon and mechanisms to elucidate roles of mechanical signals for multiple key aspects of basic cell behavior, especially cell growth. We performed live cell imaging of cells spreading on fibronectin coated micropillars to understand adhesion formation, adhesion regulation, and their impact on cell behavior. One of the earliest molecules to arrive at an adhesion formation site is a mechanosensitive protein called talin which binds to several other entities to form the backbone of focal adhesions. We found a novel role of talin cleavage, which previously was thought to play a role only in focal adhesion turnover. We found that talin cleavage is a force dependent process that regulates proper adhesion formation, thereby governing several critical cellular processes. In the absence of this talin cleavage, cells formed abnormal adhesions and showed inhibited growth. Further, we found that upon inhibition of talin cleavage, one of the key cellular behaviors of increased cellular motility upon stimulation by epidermal growth factor seemed to disappear. Epidermal growth factor receptor is a transmembrane protein and has previously been shown to play important role in various cancers where cells exhibit altered rigidity sensing. Surprisingly, we found that epidermal growth factor receptor was required for cellular rigidity sensing only on rigid substrates, highlighting the importance of the interplay between mechanical and biochemical signals in determining cell behavior.

Subjects/Keywords: Biomedical engineering; Focal adhesions; Molecular biology; Biomechanics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Saxena, M. (2018). Mechanisms of Focal Adhesions. (Doctoral Dissertation). Columbia University. Retrieved from https://doi.org/10.7916/D8NZ9R3F

Chicago Manual of Style (16^th Edition):

Saxena, Mayur. “Mechanisms of Focal Adhesions.” 2018. Doctoral Dissertation, Columbia University. Accessed January 15, 2021. https://doi.org/10.7916/D8NZ9R3F.

MLA Handbook (7^th Edition):

Saxena, Mayur. “Mechanisms of Focal Adhesions.” 2018. Web. 15 Jan 2021.

Vancouver:

Saxena M. Mechanisms of Focal Adhesions. [Internet] [Doctoral dissertation]. Columbia University; 2018. [cited 2021 Jan 15]. Available from: https://doi.org/10.7916/D8NZ9R3F.

Council of Science Editors:

Saxena M. Mechanisms of Focal Adhesions. [Doctoral Dissertation]. Columbia University; 2018. Available from: https://doi.org/10.7916/D8NZ9R3F

Universitat Pompeu Fabra

10. Mrkonjić, Sanela, 1983-. TRPV4 channel regulation and involvement in cell motility.

Degree: Departament de Ciències Experimentals i de la Salut, 2014, Universitat Pompeu Fabra

URL: http://hdl.handle.net/10803/300754

► El canal TRPV4 es un canal catiónico capaz de generar señales intracelulares de Ca2+ en diversos tejidos. La participación del canal TRPV4 en procesos de… (more)

Subjects/Keywords: Calcium; TRPV4; Hypotonicity; PIP2; Cell migration; Foal adhesions; Calci; Hipotonicitat; Migració cel·lular; Adhesions focals; 576

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mrkonjić, Sanela, 1. (2014). TRPV4 channel regulation and involvement in cell motility. (Thesis). Universitat Pompeu Fabra. Retrieved from http://hdl.handle.net/10803/300754

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mrkonjić, Sanela, 1983-. “TRPV4 channel regulation and involvement in cell motility.” 2014. Thesis, Universitat Pompeu Fabra. Accessed January 15, 2021. http://hdl.handle.net/10803/300754.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mrkonjić, Sanela, 1983-. “TRPV4 channel regulation and involvement in cell motility.” 2014. Web. 15 Jan 2021.

Vancouver:

Mrkonjić, Sanela 1. TRPV4 channel regulation and involvement in cell motility. [Internet] [Thesis]. Universitat Pompeu Fabra; 2014. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/10803/300754.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mrkonjić, Sanela 1. TRPV4 channel regulation and involvement in cell motility. [Thesis]. Universitat Pompeu Fabra; 2014. Available from: http://hdl.handle.net/10803/300754

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Manchester

11. Balloi, Eleonora. Investigating conformational changes of proteins using Förster Resonance Energy Transfer.

Degree: PhD, 2015, University of Manchester

URL: https://www.research.manchester.ac.uk/portal/en/theses/investigating-conformational-changes-of-proteins-using-frster-resonance-energy-transfer(fd5f53e7-9464-40bf-9c87-6fbd0a3d8804).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.756796

► Förster Resonance Energy Transfer (FRET)-based techniques are gaining an increasing importance in cell biology and cell-matrix adhesion studies because they allow both the detection of… (more)

▼ Förster Resonance Energy Transfer (FRET)-based techniques are gaining an increasing importance in cell biology and cell-matrix adhesion studies because they allow both the detection of conformational changes of target proteins and their localisation in cells. Frequency Domain-Fluorescence Lifetime Microscopy (FD-FLIM) is currently considered one of the most reliable methods to measure FRET in live cells. However, due to its dependence on many technical prerequisites, its use is not yet widespread. The purpose of this work was to first establish FD-FLIM measurements of FRET on a new FD-FLIM microscope module. Then we aimed to apply FD-FLIM-FRET measurements to the study of conformational changes of the cell matrix-adhesion proteins vinculin and integrin and of the growth factor receptor Tie-2. In the first part of the work, published FRET probes including distance-sensors and two sets of vinculin-based probes were extensively tested with FD-FLIM, sensitised emission and ratiometric FRET. FD-FLIM was shown to be the most accurate method in approximating molecular distances between fluorophores. Moreover this study unveiled specific caveats associated with both existing vinculin FRET probes. FD-FLIM was then used to study conformational changes of the extracellular matrix receptor alphavβ3 integrin and of the angiopoietin receptor Tie-2 using specific FRET probes designed by us. While data showed that the alphav-integrin-FRET probe localised to adhesion sites, more experiments will be required to evaluate its full functionality. The Tie-2-FRET probe was fully functional and, upon ligand binding, allowed the detection of a bending movement of the extracellular domain towards the cell membrane. Finally, a combination of FRET, immunofluorescence and tension release experiments were used to show that intracellular tension is not required to maintain integrins in their activated conformation. However, intracellular tension is required to recruit other key proteins such as vinculin, talin and tensin to adhesions sites. Overall this work demonstrates the importance of FD-FLIM-FRET as a tool to investigate conformational changes of adhesion proteins and transmembrane receptors within the cell environment.

Subjects/Keywords: 571.6; focal adhesions; fibrillar adhesions; integrin; vinculin; Tie-2; FRET; FD-FLIM

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Balloi, E. (2015). Investigating conformational changes of proteins using Förster Resonance Energy Transfer. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/investigating-conformational-changes-of-proteins-using-frster-resonance-energy-transfer(fd5f53e7-9464-40bf-9c87-6fbd0a3d8804).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.756796

Chicago Manual of Style (16^th Edition):

Balloi, Eleonora. “Investigating conformational changes of proteins using Förster Resonance Energy Transfer.” 2015. Doctoral Dissertation, University of Manchester. Accessed January 15, 2021. https://www.research.manchester.ac.uk/portal/en/theses/investigating-conformational-changes-of-proteins-using-frster-resonance-energy-transfer(fd5f53e7-9464-40bf-9c87-6fbd0a3d8804).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.756796.

MLA Handbook (7^th Edition):

Balloi, Eleonora. “Investigating conformational changes of proteins using Förster Resonance Energy Transfer.” 2015. Web. 15 Jan 2021.

Vancouver:

Balloi E. Investigating conformational changes of proteins using Förster Resonance Energy Transfer. [Internet] [Doctoral dissertation]. University of Manchester; 2015. [cited 2021 Jan 15]. Available from: https://www.research.manchester.ac.uk/portal/en/theses/investigating-conformational-changes-of-proteins-using-frster-resonance-energy-transfer(fd5f53e7-9464-40bf-9c87-6fbd0a3d8804).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.756796.

Council of Science Editors:

Balloi E. Investigating conformational changes of proteins using Förster Resonance Energy Transfer. [Doctoral Dissertation]. University of Manchester; 2015. Available from: https://www.research.manchester.ac.uk/portal/en/theses/investigating-conformational-changes-of-proteins-using-frster-resonance-energy-transfer(fd5f53e7-9464-40bf-9c87-6fbd0a3d8804).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.756796

University of Lund

12. Åkerberg, Daniel. Differently charged polypeptides and their impact on peritoneal and pleural postoperative adhesion formation.

Degree: 2013, University of Lund

URL: https://lup.lub.lu.se/record/4175114 ; https://portal.research.lu.se/ws/files/4028130/4175123.pdf

► Organization LUND UNIVERSITY Department of Surgery, Clinical Sciences Skånes University Hospital Lund SE-221 85 Lund Sweden Document name DOCTORAL DISSERTATION Date of issue: November 8,… (more)

▼ Organization LUND UNIVERSITY Department of Surgery, Clinical Sciences Skånes University Hospital Lund SE-221 85 Lund Sweden Document name DOCTORAL DISSERTATION Date of issue: November 8, 2013 Author(s): Daniel Åkerberg Sponsoring organization Title and subtitle: Differently charged polypeptides and their impact on peritoneal and pleural postoperative adhesion formation Abstract: Abdominal adhesions are formed after previous peritoneal traumas where previous surgery poses the most frequent cause. An increasing number of clinical complications due to adhesions have been detected such as small bowel obstructions, female infertility, and pain. Postoperative adhesions also form in pleura and pericardium after thoracic surgery. Complications include risk of bleeding, organ perforation and prolonged surgery, both in the thorax and abdomen, during reoperations. Previous reports have shown increased healthcare expenditures due to complications of abdominal adhesions. Several prophylactic anti-adhesion devices exist on the market, but none of them are sufficient in every aspect, such as being able to be used during abdominal infections, bleeding and in case of an intestinal anastomosis. The use of two differently charged polypeptides covering the peritoneal wounds during surgery has, in previous studies, shown promising anti-adhesion effects. The aim of this study was to investigate whether the polypeptides in any way affected different important healing aspects of the peritoneum and if the polypeptides may be administered as a spray in an animal study (I). Furthermore, the aim was to elucidate if the administered polypeptides affected important aspects of the healing process during an extended time dynamic pattern (II). It was also investigated whether the polypeptides reduced adhesions after adhesiolysis in the abdomen (III) and pleura (IV), and if there was an impact on peritoneal/pleural healing. In order to investigate the impact of polypeptides, an in vitro cell model was set up (V). A significant decrease in adhesions was seen both in the abdomen and pleura using the polypeptides. A significant decrease in adhesion reformation was seen after adhesiolysis and polypeptide administration. Despite some variation, no significant impact on key parameters of peritoneal and pleural resolving processes were seen after administration of the polypeptides. It was feasible to administer the polypeptides with a spray atomizer. Cell proliferation was decreased when higher concentrations of the polypeptides were administered, indicating a dose response relationship relying on the configuration and amount of charges of the polypeptides. In conclusion, the use of the two differently charged polypeptides to prevent abdominal and pleural adhesions was feasible, reducing adhesions after primary surgery and relaparotomy, without affecting key parameters of the resolving process investigated. Key words: Abdominal adhesions, small bowel obstructions, pain, female infertility, pleural adhesions, peritoneal, pleural, resolving process and key…

Subjects/Keywords: Surgery; peritoneal; pleural adhesions; female infertility; pain; Abdominal adhesions; small bowel obstructions; pleural; resolving process and key substances

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Åkerberg, D. (2013). Differently charged polypeptides and their impact on peritoneal and pleural postoperative adhesion formation. (Doctoral Dissertation). University of Lund. Retrieved from https://lup.lub.lu.se/record/4175114 ; https://portal.research.lu.se/ws/files/4028130/4175123.pdf

Chicago Manual of Style (16^th Edition):

Åkerberg, Daniel. “Differently charged polypeptides and their impact on peritoneal and pleural postoperative adhesion formation.” 2013. Doctoral Dissertation, University of Lund. Accessed January 15, 2021. https://lup.lub.lu.se/record/4175114 ; https://portal.research.lu.se/ws/files/4028130/4175123.pdf.

MLA Handbook (7^th Edition):

Åkerberg, Daniel. “Differently charged polypeptides and their impact on peritoneal and pleural postoperative adhesion formation.” 2013. Web. 15 Jan 2021.

Vancouver:

Åkerberg D. Differently charged polypeptides and their impact on peritoneal and pleural postoperative adhesion formation. [Internet] [Doctoral dissertation]. University of Lund; 2013. [cited 2021 Jan 15]. Available from: https://lup.lub.lu.se/record/4175114 ; https://portal.research.lu.se/ws/files/4028130/4175123.pdf.

Council of Science Editors:

Åkerberg D. Differently charged polypeptides and their impact on peritoneal and pleural postoperative adhesion formation. [Doctoral Dissertation]. University of Lund; 2013. Available from: https://lup.lub.lu.se/record/4175114 ; https://portal.research.lu.se/ws/files/4028130/4175123.pdf

13. Συμεωνίδης, Κρέων. Πειραματική μελέτη της ανάπτυξης συμφύσεων μετά την τοποθέτηση ενδοπεριτοναϊκά διαφόρων πλαστικών υλικών.

Degree: 2013, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/44668

►

Background. Adhesion formation caused by the use of foreign materials is a major medical and financial problem. The aim of this study is to compare… (more)

▼

Background. Adhesion formation caused by the use of foreign materials is a major medical and financial problem. The aim of this study is to compare the histological findings in intraperitoneal adhesion development due to long standing tubes, used in common surgical practice, in a rat model. Methods. Pieces of Polyurethane, Teflon, Silicone and Polyvinyl Chloride (PVC) from commercial available intravenous catheters (7F) were placed intraperitoneally in thirty-six female Wistar rats randomly assigned to four groups. Three months later, histologic evaluation by two independent pathologists was done blindly. The number of organs involved was examined, and the degree of inflammatory infiltration, thickness, extent and density of the adhesions were graded in a score system. Results. The overall adhesion score differed significantly (p<0.01) among the groups, the highest being in the Teflon group (mean: 13.2), followed by the PVC group (10), the silicone group (9.6) and the polyurethane group which was the lowest (6.8). Discussion. Polyurethane caused the least adhesion development in this experimental model than the other materials and therefore can be used more extensively for the construction of catheters for intraperitoneal use. This is clinically important in cases where indwelling intra-peritoneal catheters for prolonged use are necessary.

Σκοπός της μελέτης είναι η σύγκριση της δημιουργίας συμφύσεων, σε ένα πειραματικό μοντέλο, μετά από την ενδοπεριτοναϊκή τοποθέτηση τεσσάρων υλικών, τα οποία χρησιμοποιούνται για τη κατασκευή ενδοπεριτοναϊκών καθετήρων. Τμήματα πολυουρεθανίου, τεφλόν, σιλικόνης και πολυβινυλοχλωριδίου (PVC) τοποθετήθηκαν ενδοπεριτοναϊκά σε 36 θηλυκούς αρουραίους τύπου Wistar. Η ανάπτυξη συμφύσεων εκτιμήθηκε ποσοτικά με τη κλίμακα Nair. To πολυουρεθάνιο προκάλεσε το μικρότερο σχηματισμό συμφύσεων ενώ υψηλότερη βαθμολογία στη κλίμακα Nair έλαβαν τα πειραματόζωα της ομάδας πολυβινυλοχλωριδίου PVC. Όταν συγκρίναμε το πάχος και τη σταθερότητα των συμφύσεων ανάμεσα στις τέσσερις ομάδες, παρατηρήθηκαν σημαντικές διαφορές, με την ομάδα του πολυουρεθανίου να παρουσιάζει λεπτές και χαλαρές συμφύσεις. Αντίθετα, σχεδόν όλα τα πειραματόζωα της ομάδας τεφλόν ανέπτυξαν παχιές και πυκνές συμφύσεις. Συμπερασματικά, το πολυουρεθάνιο προκάλεσε τη μικρότερη δημιουργία συμφύσεων σε σχέση με τα υπόλοιπα υλικά και μπορεί να χρησιμοποιηθεί ευρύτερα για τη κατασκευή ενδοπεριτοναϊκών καθετήρων παρατεταμένης χρήσης.

Subjects/Keywords: Καθετήρες; Ενδοπεριτοναϊκές συμφύσεις; Πολυμερή υλικά; Πολυουρεθάνιο; Πολυβινυλοχλωρίδιο; Σιλικόνη; Τεφλόν; Catheters; Intraperitoneal adhesions; Polymeric materials; Polyurethane; Polyvinylchloride; Silicone; Teflon; Adhesions

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Συμεωνίδης, . �. (2013). Πειραματική μελέτη της ανάπτυξης συμφύσεων μετά την τοποθέτηση ενδοπεριτοναϊκά διαφόρων πλαστικών υλικών. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/44668

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Συμεωνίδης, Κρέων. “Πειραματική μελέτη της ανάπτυξης συμφύσεων μετά την τοποθέτηση ενδοπεριτοναϊκά διαφόρων πλαστικών υλικών.” 2013. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed January 15, 2021. http://hdl.handle.net/10442/hedi/44668.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Συμεωνίδης, Κρέων. “Πειραματική μελέτη της ανάπτυξης συμφύσεων μετά την τοποθέτηση ενδοπεριτοναϊκά διαφόρων πλαστικών υλικών.” 2013. Web. 15 Jan 2021.

Vancouver:

Συμεωνίδης �. Πειραματική μελέτη της ανάπτυξης συμφύσεων μετά την τοποθέτηση ενδοπεριτοναϊκά διαφόρων πλαστικών υλικών. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2013. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/10442/hedi/44668.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Συμεωνίδης �. Πειραματική μελέτη της ανάπτυξης συμφύσεων μετά την τοποθέτηση ενδοπεριτοναϊκά διαφόρων πλαστικών υλικών. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2013. Available from: http://hdl.handle.net/10442/hedi/44668

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

14. Hu, Yinghao; Yamashita, Keigo; Tabayashi, Nobuoki; Abe, Takehisa; Hayata, Yoshihiro; Hirose, Tomoaki; Hiraga, Shun; Tojo, Takashi; Suzuki, Shuko; Ikada, Yoshito. Gelatin Sealing Sheet for Arterial Hemostasis and Anti-adhesion in Vascular Surgery: a Dog Model Study. : 心臓血管外科領域におけるゼラチンシートの止血効果、癒着防止効果の検討.

Degree: 博士（医学）, 2015, Nara Medical University / 奈良県立医科大学

URL: http://hdl.handle.net/10564/2963

►

BACKGROUND:The bi1ayer gelatin sealing sheet was developed as a safe, effective, easy-to-handle and low-cost hemostatic agent. OBJECTIVE:To examine the feasibility of gelatin sealing sheets using… (more)

Subjects/Keywords: gelatin; fibrin glue; surgical sealant; anti-adhesion; postoperative adhesions

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APA (6^th Edition):

Hu, Yinghao; Yamashita, Keigo; Tabayashi, Nobuoki; Abe, Takehisa; Hayata, Yoshihiro; Hirose, Tomoaki; Hiraga, Shun; Tojo, Takashi; Suzuki, Shuko; Ikada, Y. (2015). Gelatin Sealing Sheet for Arterial Hemostasis and Anti-adhesion in Vascular Surgery: a Dog Model Study. : 心臓血管外科領域におけるゼラチンシートの止血効果、癒着防止効果の検討. (Thesis). Nara Medical University / 奈良県立医科大学. Retrieved from http://hdl.handle.net/10564/2963

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hu, Yinghao; Yamashita, Keigo; Tabayashi, Nobuoki; Abe, Takehisa; Hayata, Yoshihiro; Hirose, Tomoaki; Hiraga, Shun; Tojo, Takashi; Suzuki, Shuko; Ikada, Yoshito. “Gelatin Sealing Sheet for Arterial Hemostasis and Anti-adhesion in Vascular Surgery: a Dog Model Study. : 心臓血管外科領域におけるゼラチンシートの止血効果、癒着防止効果の検討.” 2015. Thesis, Nara Medical University / 奈良県立医科大学. Accessed January 15, 2021. http://hdl.handle.net/10564/2963.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hu, Yinghao; Yamashita, Keigo; Tabayashi, Nobuoki; Abe, Takehisa; Hayata, Yoshihiro; Hirose, Tomoaki; Hiraga, Shun; Tojo, Takashi; Suzuki, Shuko; Ikada, Yoshito. “Gelatin Sealing Sheet for Arterial Hemostasis and Anti-adhesion in Vascular Surgery: a Dog Model Study. : 心臓血管外科領域におけるゼラチンシートの止血効果、癒着防止効果の検討.” 2015. Web. 15 Jan 2021.

Vancouver:

Hu, Yinghao; Yamashita, Keigo; Tabayashi, Nobuoki; Abe, Takehisa; Hayata, Yoshihiro; Hirose, Tomoaki; Hiraga, Shun; Tojo, Takashi; Suzuki, Shuko; Ikada Y. Gelatin Sealing Sheet for Arterial Hemostasis and Anti-adhesion in Vascular Surgery: a Dog Model Study. : 心臓血管外科領域におけるゼラチンシートの止血効果、癒着防止効果の検討. [Internet] [Thesis]. Nara Medical University / 奈良県立医科大学; 2015. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/10564/2963.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hu, Yinghao; Yamashita, Keigo; Tabayashi, Nobuoki; Abe, Takehisa; Hayata, Yoshihiro; Hirose, Tomoaki; Hiraga, Shun; Tojo, Takashi; Suzuki, Shuko; Ikada Y. Gelatin Sealing Sheet for Arterial Hemostasis and Anti-adhesion in Vascular Surgery: a Dog Model Study. : 心臓血管外科領域におけるゼラチンシートの止血効果、癒着防止効果の検討. [Thesis]. Nara Medical University / 奈良県立医科大学; 2015. Available from: http://hdl.handle.net/10564/2963

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

15. Chambliss, Allison B. NUCLEOCYTOSKELETAL CONNECTIONS IN MECHANOTRANSDUCTION.

Degree: 2014, Johns Hopkins University

URL: http://jhir.library.jhu.edu/handle/1774.2/38019

► Cells continuously sense and respond to their physical surroundings through their cytoskeleton. The perinuclear actin cap (or actin cap) is a recently-characterized cytoskeletal organelle composed… (more)

▼ Cells continuously sense and respond to their physical surroundings through their cytoskeleton. The perinuclear actin cap (or actin cap) is a recently-characterized cytoskeletal organelle composed of thick, parallel, and highly contractile actomyosin filaments that are specifically anchored to the apical surface of the interphase nucleus. The actin cap is present in many types of adherent eukaryotic cells but is disrupted in several human disease models, including laminopathies and cancer. Through its large terminating focal adhesions and anchorage to the nuclear lamina and nuclear envelope through LINC (Linkers of the Nucleoskeleton to the Cytoskeleton) complexes, the perinuclear actin cap plays a critical role in mechanotransduction, the ability of cells to sense and respond to mechanical forces. In this work, I demonstrate that only fibers of the actin cap, not conventional basal actin stress fibers, form in response to low physiological mechanical stresses in adherent fibroblasts. While conventional basal stress fibers form only past a threshold shear stress of 0.5 dyn/cm2, actin-cap fibers form at shear stresses 50 times lower and orders-of-magnitude faster than biochemical stimulation. This fast differential response is uniquely mediated by focal adhesion protein zyxin at low shear stress and actomyosin contractility of the actin cap. I identify additional roles for lamin A/C of the nuclear lamina and LINC molecules nesprin2giant and nesprin3, which anchor actin cap fibers to the nucleus. I briefly explore mechanotransduction of interstitial fluid flow within a three-dimensional culture system. Next, I seek to characterize the extent of mechanotransduction in the nucleus through a novel microscopy assay that rapidly quantifies global acetylation on histone H3 and measures several cell and nuclear properties, including cell and nuclear morphology descriptors, cell-cycle phase, and filamentous-actin content of thousands of cells simultaneously, without cell detachment from the substrate, at single-cell resolution. These measurements reveal that isogenic, isotypic cells of identical DNA content and the same cell-cycle phase can still display large variations in H3 acetylation and that these variations correlate with specific phenotypic variations, in particular, nuclear size and actin cytoskeleton content, but not cell shape. The dependence of cell and nuclear properties on cell-cycle phase is assessed without artifact-prone cell synchronization. To further demonstrate the versatility of this assay, I quantify the complex interplay among cell cycle, epigenetic modifications, and phenotypic variations following pharmacological treatments targeting DNA integrity, cell cycle, and chromatin-modifying enzymes. Finally, recent literature suggests that the actin filament network regulates epigenetics that determine DNA packing in the nucleus and ultimately gene transcription, especially by way of histone modifications. However, a molecular mechanism underlying these cytoskeleton-based histone modifications is missing.… Advisors/Committee Members: Green, Jordan (advisor).

Subjects/Keywords: actin cap; nuclear membrane; focal adhesions; chromatin modifications; shear flow

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chambliss, A. B. (2014). NUCLEOCYTOSKELETAL CONNECTIONS IN MECHANOTRANSDUCTION. (Thesis). Johns Hopkins University. Retrieved from http://jhir.library.jhu.edu/handle/1774.2/38019

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chambliss, Allison B. “NUCLEOCYTOSKELETAL CONNECTIONS IN MECHANOTRANSDUCTION.” 2014. Thesis, Johns Hopkins University. Accessed January 15, 2021. http://jhir.library.jhu.edu/handle/1774.2/38019.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chambliss, Allison B. “NUCLEOCYTOSKELETAL CONNECTIONS IN MECHANOTRANSDUCTION.” 2014. Web. 15 Jan 2021.

Vancouver:

Chambliss AB. NUCLEOCYTOSKELETAL CONNECTIONS IN MECHANOTRANSDUCTION. [Internet] [Thesis]. Johns Hopkins University; 2014. [cited 2021 Jan 15]. Available from: http://jhir.library.jhu.edu/handle/1774.2/38019.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chambliss AB. NUCLEOCYTOSKELETAL CONNECTIONS IN MECHANOTRANSDUCTION. [Thesis]. Johns Hopkins University; 2014. Available from: http://jhir.library.jhu.edu/handle/1774.2/38019

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

16. Karandreas, Anastasios. Μείωση των μετεγχειρητικών ενδοκοιλιακών συμφύσεων με τη χρήση του methylene blue.

Degree: 2020, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/47472

►

In the present study, 26 human carcasses were studied, but because of their necrotizing material we could not study the ''methylene blue'' to be studied… (more)

Subjects/Keywords: Ενδοκοιλιακές συμφύσεις; Μετεγχειρητικές επιπλοκές; Μπλε του μεθυλενίου; Peritoneal adhesions; Methylene blue

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APA (6^th Edition):

Karandreas, A. (2020). Μείωση των μετεγχειρητικών ενδοκοιλιακών συμφύσεων με τη χρήση του methylene blue. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/47472

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Karandreas, Anastasios. “Μείωση των μετεγχειρητικών ενδοκοιλιακών συμφύσεων με τη χρήση του methylene blue.” 2020. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed January 15, 2021. http://hdl.handle.net/10442/hedi/47472.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Karandreas, Anastasios. “Μείωση των μετεγχειρητικών ενδοκοιλιακών συμφύσεων με τη χρήση του methylene blue.” 2020. Web. 15 Jan 2021.

Vancouver:

Karandreas A. Μείωση των μετεγχειρητικών ενδοκοιλιακών συμφύσεων με τη χρήση του methylene blue. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2020. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/10442/hedi/47472.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Karandreas A. Μείωση των μετεγχειρητικών ενδοκοιλιακών συμφύσεων με τη χρήση του methylene blue. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2020. Available from: http://hdl.handle.net/10442/hedi/47472

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Vanderbilt University

17. Hummel, Stephen Gunther. Novel Approaches to Time-Lapsed Microscopy Image Analysis and Detection of Biological Agent.

Degree: MS, Chemical and Physical Biology, 2014, Vanderbilt University

URL: http://hdl.handle.net/1803/10893

► This Thesis is comprised of four chapters that, respectively: 1) Examine the potential value of studying cellular dynamics and heterogeneity in the context of emerging… (more)

▼ This Thesis is comprised of four chapters that, respectively: 1) Examine the potential value of studying cellular dynamics and heterogeneity in the context of emerging biological warfare threats; 2) Review both the advantages and disadvantages of current methods that track cells using time-lapse live-cell microscopy; 3) Propose a novel algorithm to measure and track changes in cellular behavior dynamically; and 4) Highlight a novel semi-automated algorithm developed to identify and track cellular focal adhesions overtime in 3D. The ability of non-scientists to create and deploy a biological weapon (BW) highlights the emergence of a new threat, the biohacker. The advent of modern technologies enables the biohacker to employ one or a multitude of strategies to increase the tactical or strategic effectiveness of a biological agent. Preventing a successful large scale BW attack relies on the ability to rapidly detect the presence of and rapidly identify a biological weapon agent. The impact of BW agents on human health directly percolates from organ failure and tissue destruction, but is ultimately defined by the toxic effects on cellular functions, with the most severe being cell death. Understanding cellular dynamics and heterogeneity are therefore critical to immediately understanding BW effects. Time-lapsed live-cell microscopy is an ideal technique to study cellular changes. However, tracking methods are limited. Initial integer programming algorithms were successful in tracking cells but are limited to low cell number and density. Others handle large cell numbers but are limited in their capability to detect mitotic events. There exist three distinct and novel components to our approach: 1) user corrected segmentation, 2) a k-nearest neighbor algorithm to generate “high confidence tracks”, and 3) track assumptions automatically scrutinized within the program. Initial results indicates that the algorithm is accurate, repeatable, and applicable to a variety of datasets. The algorithm provides a surfeit of quantifiable data on cell morphological features. Though the results of the semi-automated focal adhesion (FA) algorithm are at an early stage, the potential for a fully automated algorithm is evident. The initial data indicate correct identification of FAs is being achieved. Advisors/Committee Members: Carlos Lopez (committee member), Thomas Yankeelov (committee member), Vito Quaranta (Committee Chair).

Subjects/Keywords: focal adhesions; biological agent; detection; Image Microscopy; bio-digital canary

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APA (6^th Edition):

Hummel, S. G. (2014). Novel Approaches to Time-Lapsed Microscopy Image Analysis and Detection of Biological Agent. (Thesis). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/10893

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hummel, Stephen Gunther. “Novel Approaches to Time-Lapsed Microscopy Image Analysis and Detection of Biological Agent.” 2014. Thesis, Vanderbilt University. Accessed January 15, 2021. http://hdl.handle.net/1803/10893.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hummel, Stephen Gunther. “Novel Approaches to Time-Lapsed Microscopy Image Analysis and Detection of Biological Agent.” 2014. Web. 15 Jan 2021.

Vancouver:

Hummel SG. Novel Approaches to Time-Lapsed Microscopy Image Analysis and Detection of Biological Agent. [Internet] [Thesis]. Vanderbilt University; 2014. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/1803/10893.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hummel SG. Novel Approaches to Time-Lapsed Microscopy Image Analysis and Detection of Biological Agent. [Thesis]. Vanderbilt University; 2014. Available from: http://hdl.handle.net/1803/10893

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Penn State University

18. Lee, Deborah Snyder. Characterizing Melanoma Adhesion Dynamics: Thrombin Mediated Interactions and Polymorphic Neutrophil Deformability.

Degree: 2016, Penn State University

URL: https://submit-etda.libraries.psu.edu/catalog/29308

► In order for cancer metastasis to occur, tumor cells must separate from the primary tumor and enter the circulatory system, and then extravasate through the… (more)

▼ In order for cancer metastasis to occur, tumor cells must separate from the primary tumor and enter the circulatory system, and then extravasate through the vascular endothelial cells in order to form a secondary tumor at a distant site. This work focuses on the adhesion of melanoma cells to the endothelium in response to various conditions. Soluble fibrin serves as a cross-linker between endothelial cells and melanoma cells. We used an aptamer, a small oligonucleic acid sequence with a distinct tertiary structure, to inhibit thrombin activity, and therefore formation of soluble fibrin. The apparatus used, a parallel-plate flow chamber, allows for the perfusion of cells over a confluent monolayer, therefore modeling physiological flow conditions over the endothelium in vitro. The results indicated that the aptamer was able to effectively inhibit thrombin activity and subsequent melanoma adhesions decreased after addition of the aptamer. Melanoma interactions involving the effects of activation and sequestration of polymorphic neutrophils (PMNs) were also investigated. PMN activation and sequestration has been associated with inflammation of the pulmonary microcirculation. It was found that activation, which triggers changes in PMN deformability, has a significant effect on the aggregation of melanoma cells on the endothelium whereas environments with high concentrations of PMNs did not yield significant changes. The results suggested that activation plays a key role in aiding metastatic melanoma cells in adhering to the endothelium due to a decrease in neutrophil deformability. This effect was reversed by the addition of cytochalasin B, a mycotoxin that disrupts actin filaments and increases deformability of PMNs, and a decrease in melanoma aggregations on the endothelium was observed. Thus, these results suggest that deformability plays a larger role than accumulation of PMNs in aiding melanoma cells to adhere to the endothelium. Advisors/Committee Members: Cheng Dong, Thesis Advisor/Co-Advisor.

Subjects/Keywords: cancer; melanoma; thrombin; fibrinogen; fibrin; polymorphic neutrophils; cellular adhesions; flow assays

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APA (6^th Edition):

Lee, D. S. (2016). Characterizing Melanoma Adhesion Dynamics: Thrombin Mediated Interactions and Polymorphic Neutrophil Deformability. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/29308

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lee, Deborah Snyder. “Characterizing Melanoma Adhesion Dynamics: Thrombin Mediated Interactions and Polymorphic Neutrophil Deformability.” 2016. Thesis, Penn State University. Accessed January 15, 2021. https://submit-etda.libraries.psu.edu/catalog/29308.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lee, Deborah Snyder. “Characterizing Melanoma Adhesion Dynamics: Thrombin Mediated Interactions and Polymorphic Neutrophil Deformability.” 2016. Web. 15 Jan 2021.

Vancouver:

Lee DS. Characterizing Melanoma Adhesion Dynamics: Thrombin Mediated Interactions and Polymorphic Neutrophil Deformability. [Internet] [Thesis]. Penn State University; 2016. [cited 2021 Jan 15]. Available from: https://submit-etda.libraries.psu.edu/catalog/29308.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lee DS. Characterizing Melanoma Adhesion Dynamics: Thrombin Mediated Interactions and Polymorphic Neutrophil Deformability. [Thesis]. Penn State University; 2016. Available from: https://submit-etda.libraries.psu.edu/catalog/29308

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Boston University

19. Minns, Martin Scott. The role of the P2X7 receptor in injury-induced calcium dynamics and cell migration in the corneal epithelium.

Degree: PhD, Biochemistry, 2015, Boston University

URL: http://hdl.handle.net/2144/16225

► Wound healing in the corneal epithelium is an essential process to maintain corneal clarity and organism health. The earliest events of cellular injury response include… (more)

▼ Wound healing in the corneal epithelium is an essential process to maintain corneal clarity and organism health. The earliest events of cellular injury response include the release of nucleotides and the activation of P2 purinergic receptors. While the purinergic receptor P2X7 has been shown to promote cell migration, its role in corneal epithelial wound healing is still poorly understood. The goal of this work is to better understand the role of P2X7 in the injury response. We analyzed P2X7 expression after epithelial injury in rat corneal organ cultures and found that the receptor localizes to the leading edge of the corneal epithelium. However, overall mRNA and protein expression of P2X7 decreased after injury. Inhibition of P2X7 activation significantly delayed wound closure and prevented the leading edge-localization after injury. We found that P2X7 inhibition altered the wound-induced calcium wave in epithelial cells and altered the number and distribution of focal adhesions in the migrating cells. Live cell imaging of epithelial cells showed that P2X7 inhibition led to altered actin rearrangement, with thick actin bundles in the treated cells. In order to determine the importance of P2X7 in epithelial differentiation and stratified cell migration, we developed a stratified culture model. The cells in the stratified model expressed proliferative and differentiation markers similar to organ cultured corneas, as well as similar P2X7 expression and localization after injury. Together, these results show the importance of P2X7 in the overall purinergic response to injury, and provide tools to study P2X7 in stratified corneal cell migration. To determine if P2X7 may contribute to pathologic delayed wound healing in diseases such as type 2 diabetes, we analyzed P2X7 expression in diabetic human corneas and diabetic model rodent corneas. We showed that P2X7 expression is significantly elevated in unwounded diabetic corneas, and that wound healing is delayed in the rodent model. These data show that elevated P2X7 expression may contribute to the delayed healing in disease and may be a possible therapeutic target.

Subjects/Keywords: Cellular biology; Calcium; Cell migration; Cornea; Epithelium; Focal adhesions; Purinergic receptors

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Minns, M. S. (2015). The role of the P2X7 receptor in injury-induced calcium dynamics and cell migration in the corneal epithelium. (Doctoral Dissertation). Boston University. Retrieved from http://hdl.handle.net/2144/16225

Chicago Manual of Style (16^th Edition):

Minns, Martin Scott. “The role of the P2X7 receptor in injury-induced calcium dynamics and cell migration in the corneal epithelium.” 2015. Doctoral Dissertation, Boston University. Accessed January 15, 2021. http://hdl.handle.net/2144/16225.

MLA Handbook (7^th Edition):

Minns, Martin Scott. “The role of the P2X7 receptor in injury-induced calcium dynamics and cell migration in the corneal epithelium.” 2015. Web. 15 Jan 2021.

Vancouver:

Minns MS. The role of the P2X7 receptor in injury-induced calcium dynamics and cell migration in the corneal epithelium. [Internet] [Doctoral dissertation]. Boston University; 2015. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/2144/16225.

Council of Science Editors:

Minns MS. The role of the P2X7 receptor in injury-induced calcium dynamics and cell migration in the corneal epithelium. [Doctoral Dissertation]. Boston University; 2015. Available from: http://hdl.handle.net/2144/16225

20. Schreinemacher, M.H.F. Abdominal wall hernia repair : intraperitoneal mesh and adhesions.

Degree: 2015, Maastricht University

URL: https://cris.maastrichtuniversity.nl/en/publications/bdfe13da-2e8b-47b7-b779-ace695dc4d34 ; urn:nbn:nl:ui:27-bdfe13da-2e8b-47b7-b779-ace695dc4d34 ; bdfe13da-2e8b-47b7-b779-ace695dc4d34 ; urn:isbn:9789461825308 ; urn:nbn:nl:ui:27-bdfe13da-2e8b-47b7-b779-ace695dc4d34 ; https://cris.maastrichtuniversity.nl/en/publications/bdfe13da-2e8b-47b7-b779-ace695dc4d34

► One in five patients develops an incisional hernia after having undergone abdominal surgery. Such hernias are abdominal muscle defects allowing intestines to protrude. Hernia repair… (more)

Subjects/Keywords: incisional hernias; postoperative adhesions

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APA (6^th Edition):

Schreinemacher, M. H. F. (2015). Abdominal wall hernia repair : intraperitoneal mesh and adhesions. (Doctoral Dissertation). Maastricht University. Retrieved from https://cris.maastrichtuniversity.nl/en/publications/bdfe13da-2e8b-47b7-b779-ace695dc4d34 ; urn:nbn:nl:ui:27-bdfe13da-2e8b-47b7-b779-ace695dc4d34 ; bdfe13da-2e8b-47b7-b779-ace695dc4d34 ; urn:isbn:9789461825308 ; urn:nbn:nl:ui:27-bdfe13da-2e8b-47b7-b779-ace695dc4d34 ; https://cris.maastrichtuniversity.nl/en/publications/bdfe13da-2e8b-47b7-b779-ace695dc4d34

Chicago Manual of Style (16^th Edition):

Schreinemacher, M H F. “Abdominal wall hernia repair : intraperitoneal mesh and adhesions.” 2015. Doctoral Dissertation, Maastricht University. Accessed January 15, 2021. https://cris.maastrichtuniversity.nl/en/publications/bdfe13da-2e8b-47b7-b779-ace695dc4d34 ; urn:nbn:nl:ui:27-bdfe13da-2e8b-47b7-b779-ace695dc4d34 ; bdfe13da-2e8b-47b7-b779-ace695dc4d34 ; urn:isbn:9789461825308 ; urn:nbn:nl:ui:27-bdfe13da-2e8b-47b7-b779-ace695dc4d34 ; https://cris.maastrichtuniversity.nl/en/publications/bdfe13da-2e8b-47b7-b779-ace695dc4d34.

MLA Handbook (7^th Edition):

Schreinemacher, M H F. “Abdominal wall hernia repair : intraperitoneal mesh and adhesions.” 2015. Web. 15 Jan 2021.

Vancouver:

Schreinemacher MHF. Abdominal wall hernia repair : intraperitoneal mesh and adhesions. [Internet] [Doctoral dissertation]. Maastricht University; 2015. [cited 2021 Jan 15]. Available from: https://cris.maastrichtuniversity.nl/en/publications/bdfe13da-2e8b-47b7-b779-ace695dc4d34 ; urn:nbn:nl:ui:27-bdfe13da-2e8b-47b7-b779-ace695dc4d34 ; bdfe13da-2e8b-47b7-b779-ace695dc4d34 ; urn:isbn:9789461825308 ; urn:nbn:nl:ui:27-bdfe13da-2e8b-47b7-b779-ace695dc4d34 ; https://cris.maastrichtuniversity.nl/en/publications/bdfe13da-2e8b-47b7-b779-ace695dc4d34.

Council of Science Editors:

Schreinemacher MHF. Abdominal wall hernia repair : intraperitoneal mesh and adhesions. [Doctoral Dissertation]. Maastricht University; 2015. Available from: https://cris.maastrichtuniversity.nl/en/publications/bdfe13da-2e8b-47b7-b779-ace695dc4d34 ; urn:nbn:nl:ui:27-bdfe13da-2e8b-47b7-b779-ace695dc4d34 ; bdfe13da-2e8b-47b7-b779-ace695dc4d34 ; urn:isbn:9789461825308 ; urn:nbn:nl:ui:27-bdfe13da-2e8b-47b7-b779-ace695dc4d34 ; https://cris.maastrichtuniversity.nl/en/publications/bdfe13da-2e8b-47b7-b779-ace695dc4d34

21. Berry, James Edward. The Role of Visceral Peritoneum in LPS Mediated Inflammatory Responses.

Degree: 2017, University of Manchester

URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:309005

► Peritoneal adhesions pose a considerable postoperative burden. The mechanisms underlying adhesion formation remain poorly understood but relate principally to inflammation and fibrinolysis. The peritoneum may… (more)

▼ Peritoneal adhesions pose a considerable postoperative burden. The mechanisms underlying adhesion formation remain poorly understood but relate principally to inflammation and fibrinolysis. The peritoneum may coordinate the inflammatory response that leads to adhesion formation but human adhesion formation cannot be studied directly so most research has employed animal models and individual transformed cell lines. Furthermore, the specific roles of the visceral or parietal peritoneum in adhesion formation are unclear. The aims of the studies undertaken in this thesis are to investigate the regulation of a gut bacteria-driven inflammatory response in human visceral peritoneum. A novel ex vivo model was designed using human small bowel visceral peritoneum, cultured with E. coli derived LPS, for 18 to 48 hours. ELISA and rtPCR were used to analyse supernatant protein concentration and gene expression respectively for key inflammatory mediators associated with adhesion formation including cytokines (IL-6, IL-10, TNFα), transforming growth factor-β1 (TGF-β1) and fibrinolytic factors (tPA and PAI-1) as well as the signalling receptors thought to be involved in LPS-mediated inflammation (TLR2 and 4). LPS exposure resulted in stimulation of IL-6, IL-10 and TNFα production from human visceral peritoneum at 18 hours. These increases correlated with increased gene expression for IL-6 and TNFα but not IL-10. TGF-β1 production and gene expression were unchanged at 18 & 24 hours but increased at 48 hours when co-incubated with IL-1β. PAI-1 was increased but tPA was unchanged following LPS stimulation, suggesting a reduction in peritoneal fibrinolytic activity. Peritoneal tissue expressed the signalling receptors TLR 2 and 4. However, inhibition of TLR 2 or 4 failed to abrogate production of cytokines in cultured primary mesothelial cells or peritoneal tissue post-LPS exposure. TLR 4 inhibition did reduce mesothelial cell production of chemokines, CCL2 and CXCL1. These novel findings demonstrated that human visceral peritoneum actively participates in LPS-induced peritoneal inflammatory responses and reduced fibrinolysis which may account for the visceral peritoneum’s affinity to form adhesions. Ex vivo human peritoneal culture is a novel, feasible and reproducible method of investigating the role of the peritoneum in inflammation. Advisors/Committee Members: CARLSON, GORDON GL, WARHURST, GEOFFREY G, Carlson, Gordon, Herrick, Sarah, Warhurst, Geoffrey.

Subjects/Keywords: Peritoneal inflammation; Adhesions

…Visceral Peritoneum in LPS mediated Inflammatory Responses Abstract Peritoneal adhesions pose a… …fibrinolysis which may account for the visceral peritoneum’s affinity to form adhesions. Ex vivo… …James Berry 1.1 Introduction Peritoneal adhesions are abnormal connections between the… …normal healing process and in evolutionary terms, adhesions are thought to convey a protective… …allowing abscess formation.2 However adhesions also cause significant morbidity and mortality…

10 more images…

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APA (6^th Edition):

Berry, J. E. (2017). The Role of Visceral Peritoneum in LPS Mediated Inflammatory Responses. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:309005

Chicago Manual of Style (16^th Edition):

Berry, James Edward. “The Role of Visceral Peritoneum in LPS Mediated Inflammatory Responses.” 2017. Doctoral Dissertation, University of Manchester. Accessed January 15, 2021. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:309005.

MLA Handbook (7^th Edition):

Berry, James Edward. “The Role of Visceral Peritoneum in LPS Mediated Inflammatory Responses.” 2017. Web. 15 Jan 2021.

Vancouver:

Berry JE. The Role of Visceral Peritoneum in LPS Mediated Inflammatory Responses. [Internet] [Doctoral dissertation]. University of Manchester; 2017. [cited 2021 Jan 15]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:309005.

Council of Science Editors:

Berry JE. The Role of Visceral Peritoneum in LPS Mediated Inflammatory Responses. [Doctoral Dissertation]. University of Manchester; 2017. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:309005

University of Adelaide

22. Wadham, Carol. Protein Tyrosine Phosphatase Pez : its role in the regulation of cell-cell adhesions.

Degree: 2003, University of Adelaide

URL: http://hdl.handle.net/2440/80070

► The balance of tyrosine phosphorylation in the cell is maintained by the opposing actions of protein tyrosine kinases (PTKs) and protein tyrosine phosphatases (PTPs). Investigation… (more)

▼ The balance of tyrosine phosphorylation in the cell is maintained by the opposing actions of protein tyrosine kinases (PTKs) and protein tyrosine phosphatases (PTPs). Investigation into tyrosine phosphorylation was initially focused on the action of PTKs. However, research over the past decade has revealed that PTPs also play a key role in signal transduction. The multi-protein complexes that constitute the cell-cell adhesions in endothelial and epithelial tissues are dynamically restructured in response to extracellular and intracellular signalling. Tyrosine phosphorylation is involved in the regulation of both adherens junctions and tight junctions. Inhibitors of PTPs have been shown to disrupt cell-cell adhesions indicating that PTPs are important in maintaining adhesion integrity. The maintenance of a selectively permeable barrier is an essential function of endothelial cells, which are the cells that line the lumen of blood vessels. Therefore, it is important to understand the normal functioning of the proteins in the cell-cell adhesion complexes. The aims of this research were to ascertain which members of the PTP family are expressed in human umbilical vein endothelial cells (HUVEC) and to characterise a PTP that may potentially be involved in the regulation of cell-cell adhesions. A homology screen identified a cytosolic phosphatase, PTP-Pez, to be highly expressed in HUVEC. The presence of the protein-protein interaction FERM domain (band 4.1, ezrin, radixin and moesin) at the N-terminus of Pez predicted its localisation to the plasma membrane. Specific antibodies showed that in confluent monolayers Pez is cytoplasmic and concentrated at intercellular junctions but the protein is nuclear in sub-confluent cells. The adherens junction protein β-catenin and the tight junction protein occludin were both identified as potential substrates of Pez using a "substrate-trapping" approach. Data showing that Pez bound to and dephosphorylated β-catenin in vivo further substantiated this. A truncated form of Pez lacking the catalytic domain acted as a dominant negative mutant inhibiting the dephosphorylation of its substrates at intercellular junctions and enhancing cell motility. Canine epithelial cells overexpressing Pez underwent an apparent epithelial to mesenchymal transition (EMT), a process typified by downregulation of cell-cell contacts. These findings indicate that Pez plays a role in the regulation of cell-cell adhesion. Advisors/Committee Members: Khew-Goodall, Yeesim (advisor), Dept. of Medicine (school).

Subjects/Keywords: protein; tyrosine phosphatase; adhesions

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APA (6^th Edition):

Wadham, C. (2003). Protein Tyrosine Phosphatase Pez : its role in the regulation of cell-cell adhesions. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/80070

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wadham, Carol. “Protein Tyrosine Phosphatase Pez : its role in the regulation of cell-cell adhesions.” 2003. Thesis, University of Adelaide. Accessed January 15, 2021. http://hdl.handle.net/2440/80070.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wadham, Carol. “Protein Tyrosine Phosphatase Pez : its role in the regulation of cell-cell adhesions.” 2003. Web. 15 Jan 2021.

Vancouver:

Wadham C. Protein Tyrosine Phosphatase Pez : its role in the regulation of cell-cell adhesions. [Internet] [Thesis]. University of Adelaide; 2003. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/2440/80070.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wadham C. Protein Tyrosine Phosphatase Pez : its role in the regulation of cell-cell adhesions. [Thesis]. University of Adelaide; 2003. Available from: http://hdl.handle.net/2440/80070

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Toronto

23. Mohammad, Ibrahim. Role of Flightless I in Cell Migration.

Degree: 2010, University of Toronto

URL: http://hdl.handle.net/1807/25863

►

A central process in connective tissue homeostasis is cell migration, which involves dynamic interactions between focal adhesions, the actin cytoskeleton and mitochondria, but the role… (more)

Subjects/Keywords: flightless I; cell migration; actin cytoskeleton; mitochondria; cell adhesions; 0567

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APA (6^th Edition):

Mohammad, I. (2010). Role of Flightless I in Cell Migration. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/25863

Chicago Manual of Style (16^th Edition):

Mohammad, Ibrahim. “Role of Flightless I in Cell Migration.” 2010. Masters Thesis, University of Toronto. Accessed January 15, 2021. http://hdl.handle.net/1807/25863.

MLA Handbook (7^th Edition):

Mohammad, Ibrahim. “Role of Flightless I in Cell Migration.” 2010. Web. 15 Jan 2021.

Vancouver:

Mohammad I. Role of Flightless I in Cell Migration. [Internet] [Masters thesis]. University of Toronto; 2010. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/1807/25863.

Council of Science Editors:

Mohammad I. Role of Flightless I in Cell Migration. [Masters Thesis]. University of Toronto; 2010. Available from: http://hdl.handle.net/1807/25863

University of Iowa

24. Peng, Xiao. The function and regulation of vinculin in cell-cell adhesions.

Degree: PhD, Biochemistry, 2011, University of Iowa

URL: https://ir.uiowa.edu/etd/2605

► Adherens junctions are essential for embryogenesis and tissue homeostasis. The major transmembrane adhesion receptors in adherens junctions are the cadherins, which mediate cell-cell adhesion… (more)

▼ Adherens junctions are essential for embryogenesis and tissue homeostasis. The major transmembrane adhesion receptors in adherens junctions are the cadherins, which mediate cell-cell adhesion by binding to cadherins on adjacent cells. Cadherin function is regulated by the protein complexes that assemble at its cytoplasmic tail. Vinculin is one cytoplasmic component of the cadherin adhesion complex, but unlike other junction components, it also is enriched in cell-matrix adhesions. The presence of vinculin in cellmatrix adhesions has commanded the most attention, while little is known about its role in cell-cell adhesions. To define the role of vinculin in adherens junctions, I established a short hairpin RNA-based knockdown/substitution system that perturbs vinculin preferentially at sites of cell-cell adhesion. When this system was applied to epithelial cells, cell morphology was altered, and cell-cell adhesion was reduced owing to a lack of cadherin on the cell surface. I investigated the mechanism for this effect and found that vinculin must bind to beta-catenin to regulate E-cadherin surface expression. Having established a role for vinculin in cell-cell adhesions, the critical question became how vinculin recruitment to and activation at cell-cell junctions are regulated. I found that á-catenin triggers activating vinculin conformational changes. Unlike all of the known vinculin activators in cell-matrix adhesions, alpha-catenin binds and activates vinculin independently of an A50I substitution. Thus, adherens junction activators and cell-matrix activators bind to distinct regions of vinculin to activate this molecule. Using mutant vinculins that cannot be tyrosine phosphorylated, I found that vinculin recruitment to cell-cell adhesions, but not cell-matrix adhesions, requires phosphorylation at Y822. Furthermore, this residue is phosphorylated by Abl tyrosine kinases during the assembly of cell-cell adhesions. Taken together, these studies explain how vinculin is differentially recruited to adherens junctions and cell-matrix adhesions and describes the first known role for vinculin at cell-cell adhesions. Advisors/Committee Members: DeMali, Kris A. (supervisor).

Subjects/Keywords: actin; cadherin; catenin; cell-cell adhesions; tryosine phosphorylation; vinculin; Biochemistry

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APA (6^th Edition):

Peng, X. (2011). The function and regulation of vinculin in cell-cell adhesions. (Doctoral Dissertation). University of Iowa. Retrieved from https://ir.uiowa.edu/etd/2605

Chicago Manual of Style (16^th Edition):

Peng, Xiao. “The function and regulation of vinculin in cell-cell adhesions.” 2011. Doctoral Dissertation, University of Iowa. Accessed January 15, 2021. https://ir.uiowa.edu/etd/2605.

MLA Handbook (7^th Edition):

Peng, Xiao. “The function and regulation of vinculin in cell-cell adhesions.” 2011. Web. 15 Jan 2021.

Vancouver:

Peng X. The function and regulation of vinculin in cell-cell adhesions. [Internet] [Doctoral dissertation]. University of Iowa; 2011. [cited 2021 Jan 15]. Available from: https://ir.uiowa.edu/etd/2605.

Council of Science Editors:

Peng X. The function and regulation of vinculin in cell-cell adhesions. [Doctoral Dissertation]. University of Iowa; 2011. Available from: https://ir.uiowa.edu/etd/2605

Boston University

25. Poythress, Ransom Harold. Focal adhesion protein dynamics and the role of endosomes in contractile, fully differentiated, vascular smooth muscle.

Degree: PhD, Biology, 2013, Boston University

URL: http://hdl.handle.net/2144/13144

► Turnover of focal adhesions (FAs) is known to be critical for cell migration and adhesion of proliferative vascular smooth muscle cells (VSMCs). However, it is… (more)

Subjects/Keywords: Molecular biology; Adhesions; Endosomes; Focal; Muscle; Smooth; Vascular

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APA (6^th Edition):

Poythress, R. H. (2013). Focal adhesion protein dynamics and the role of endosomes in contractile, fully differentiated, vascular smooth muscle. (Doctoral Dissertation). Boston University. Retrieved from http://hdl.handle.net/2144/13144

Chicago Manual of Style (16^th Edition):

Poythress, Ransom Harold. “Focal adhesion protein dynamics and the role of endosomes in contractile, fully differentiated, vascular smooth muscle.” 2013. Doctoral Dissertation, Boston University. Accessed January 15, 2021. http://hdl.handle.net/2144/13144.

MLA Handbook (7^th Edition):

Poythress, Ransom Harold. “Focal adhesion protein dynamics and the role of endosomes in contractile, fully differentiated, vascular smooth muscle.” 2013. Web. 15 Jan 2021.

Vancouver:

Poythress RH. Focal adhesion protein dynamics and the role of endosomes in contractile, fully differentiated, vascular smooth muscle. [Internet] [Doctoral dissertation]. Boston University; 2013. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/2144/13144.

Council of Science Editors:

Poythress RH. Focal adhesion protein dynamics and the role of endosomes in contractile, fully differentiated, vascular smooth muscle. [Doctoral Dissertation]. Boston University; 2013. Available from: http://hdl.handle.net/2144/13144

Boston University

26. Teicher, Gregory. Live cell imaging demonstrates the role of purinoreceptor P2X7 in actin cytoskeletal rearrangements and focal adhesion dynamics after injury in corneal epithelial cells.

Degree: MS, Medical Sciences, 2015, Boston University

URL: http://hdl.handle.net/2144/13981

► The cornea forms the anterior surface of the eye and is responsible for most of the eye’s refractive power. Injury to the outermost layer of… (more)

▼ The cornea forms the anterior surface of the eye and is responsible for most of the eye’s refractive power. Injury to the outermost layer of the cornea, a non-keratinized stratified squamous epithelium, triggers a transient rise in intracellular calcium concentration that propagates radially from the wound. This calcium mobilization is initiated by the binding of nucleotides such as adenosine triphosphate (ATP), which are released from cells ruptured by the injury, to purinergic receptors (purinoreceptors) on undamaged cells near the wound. Downstream effects of this injury-induced "calcium wave" are generally thought to include the activation of signaling pathways that promote wound healing. However, the specific contributions of individual purinergic receptors to the overall wound response have in most cases not been well characterized. Purinoreceptors are classified into two broad categories: the P2Y class of G protein-coupled receptors, which act through second messengers to release calcium into the cytosol from the endoplasmic reticulum, and the P2X class of ligand-gated ionotropic receptors, which release calcium into the cytosol from the extracellular environment. Previously, our lab established the importance of the P2Y2 receptor to corneal epithelial wound healing by showing that P2Y2 activation makes a substantial contribution to the overall wound-induced calcium response, particularly in cells back from the leading edge, and promotes cell migration after injury. P2Y2 activation was also found to promote the phosphorylation of proteins involved in focal adhesions, which are multi-protein complexes that facilitate cell migration by transmitting the forces generated by the actin cytoskeleton to the extracellular environment. More recently, our lab has begun to demonstrate that P2X7 may play an equally important, yet distinct and perhaps complementary role in corneal epithelial wound healing. For instance, P2X7 was found to strongly influence the intensity of the injury-induced calcium response in cells immediately adjacent to the wound, and treatment with the P2X7 inhibitor oxidized ATP (oxATP) was shown to impair migration after injury both in vitro and in ex vivo rat corneas. Additionally, immunofluorescence of cells fixed eight hours after injury revealed an altered actin cytoskeletal architecture and localization of the focal adhesion proteins talin and vinculin in oxATP-treated cells compared to control cells. The goal of the present study was to further characterize P2X7’s role in the overall response to injury by using live cell imaging to examine actin cytoskeletal rearrangements and focal adhesion dynamics after injury under both control conditions and conditions of P2X7 inhibition. Human corneal limbal epithelial (HCLE) cells were transduced to express either actin or talin tagged with green fluorescent protein (GFP), grown into confluent monolayers, and scratch wounded in the presence or absence of oxATP. Cells at the leading edge of the wound were imaged using confocal microscopy every 10…

Subjects/Keywords: Biochemistry; P2X7; Actin; Cornea; Cytoskeleton; Focal adhesions; Injury

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Teicher, G. (2015). Live cell imaging demonstrates the role of purinoreceptor P2X7 in actin cytoskeletal rearrangements and focal adhesion dynamics after injury in corneal epithelial cells. (Masters Thesis). Boston University. Retrieved from http://hdl.handle.net/2144/13981

Chicago Manual of Style (16^th Edition):

Teicher, Gregory. “Live cell imaging demonstrates the role of purinoreceptor P2X7 in actin cytoskeletal rearrangements and focal adhesion dynamics after injury in corneal epithelial cells.” 2015. Masters Thesis, Boston University. Accessed January 15, 2021. http://hdl.handle.net/2144/13981.

MLA Handbook (7^th Edition):

Teicher, Gregory. “Live cell imaging demonstrates the role of purinoreceptor P2X7 in actin cytoskeletal rearrangements and focal adhesion dynamics after injury in corneal epithelial cells.” 2015. Web. 15 Jan 2021.

Vancouver:

Teicher G. Live cell imaging demonstrates the role of purinoreceptor P2X7 in actin cytoskeletal rearrangements and focal adhesion dynamics after injury in corneal epithelial cells. [Internet] [Masters thesis]. Boston University; 2015. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/2144/13981.

Council of Science Editors:

Teicher G. Live cell imaging demonstrates the role of purinoreceptor P2X7 in actin cytoskeletal rearrangements and focal adhesion dynamics after injury in corneal epithelial cells. [Masters Thesis]. Boston University; 2015. Available from: http://hdl.handle.net/2144/13981

University of California – San Francisco

27. Kenific, Candia Marie. Regulation of metastatic phenotypes by autophagy.

Degree: Biomedical Sciences, 2015, University of California – San Francisco

URL: http://www.escholarship.org/uc/item/8s4757j5

► Metastasis, the process by which primary tumor cells disseminate throughout the body and colonize distant organs, continues to be the principal cause of cancer-associated death.… (more)

▼ Metastasis, the process by which primary tumor cells disseminate throughout the body and colonize distant organs, continues to be the principal cause of cancer-associated death. Although our understanding of the biology underlying metastasis has advanced significantly in recent years, there is still a lack of effective therapies available for treating this disease. In light of this unmet clinical need, my thesis has focused on establishing whether autophagy, a cellular stress response pathway, may be a novel therapeutic target for treating cancer metastasis. Autophagy is an evolutionarily conserved process that involves the formation of a double-membrane vesicle, the autophagosome, which consumes cellular constituents and delivers them to the lysosome for degradation. Autophagy is well-recognized to be a crucial regulator of tumorigenesis. Moreover, roles for autophagy in regulating metastatic phenotypes, such as adhesion-independent growth and survival and tumor cell invasion and migration have emerged. However, the mechanisms underlying regulation of these metastasis traits by autophagy are poorly understood, and it is not known precisely how autophagy regulates in vivo cancer metastasis. Here, I investigate the cell biological mechanisms through which autophagy regulates cell migration. I find that autophagy is required for the efficient assembly and disassembly, or turnover, of integrin-based cell-matrix focal adhesions (FAs) in motile cells, and I uncover that the autophagy cargo receptor NBR1, which mediates targeting of autophagosomes to their substrates, acts as a key mediator of cell migration and autophagy-dependent FA disassembly. Collectively, these findings point to a molecular mechanism of NBR1-mediated selective autophagy in directly regulating FA turnover during migration. I also explored the role of autophagy during in vivo cancer metastasis. Despite extensive data suggesting autophagy would function to promote metastasis, I find that autophagy functions as a metastasis suppressor. In addition, autophagy does not impact growth or viability of established metastatic tumors, suggesting it regulates stages of metastasis prior to overt secondary tumor growth. Finally, I identify the autophagy cargo receptor, NBR1, as a novel metastasis promoter. Because NBR1 is itself degraded by autophagy, overall, these results hint at an unexpected model in which NBR1 accumulation upon autophagy inhibition drives metastasis.

Subjects/Keywords: Biology; autophagy; cell migration; focal adhesions; metastasis; NBR1

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APA (6^th Edition):

Kenific, C. M. (2015). Regulation of metastatic phenotypes by autophagy. (Thesis). University of California – San Francisco. Retrieved from http://www.escholarship.org/uc/item/8s4757j5

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kenific, Candia Marie. “Regulation of metastatic phenotypes by autophagy.” 2015. Thesis, University of California – San Francisco. Accessed January 15, 2021. http://www.escholarship.org/uc/item/8s4757j5.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kenific, Candia Marie. “Regulation of metastatic phenotypes by autophagy.” 2015. Web. 15 Jan 2021.

Vancouver:

Kenific CM. Regulation of metastatic phenotypes by autophagy. [Internet] [Thesis]. University of California – San Francisco; 2015. [cited 2021 Jan 15]. Available from: http://www.escholarship.org/uc/item/8s4757j5.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kenific CM. Regulation of metastatic phenotypes by autophagy. [Thesis]. University of California – San Francisco; 2015. Available from: http://www.escholarship.org/uc/item/8s4757j5

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Western Ontario

28. To, Ciric Chi Wing. Molecular Mechanisms of Cell Migration Inhibition by Synthetic Triterpenoids.

Degree: 2012, University of Western Ontario

URL: https://ir.lib.uwo.ca/etd/480

► Cell migration is an important mediator of cancer metastasis and invasion, which is responsible for 90% of cancer-related premature deaths in Canada. Synthetic triterpenoids are… (more)

Subjects/Keywords: cell migration; triterpenoids; cytoskeleton; focal adhesions; Cell Biology

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APA (6^th Edition):

To, C. C. W. (2012). Molecular Mechanisms of Cell Migration Inhibition by Synthetic Triterpenoids. (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/480

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

To, Ciric Chi Wing. “Molecular Mechanisms of Cell Migration Inhibition by Synthetic Triterpenoids.” 2012. Thesis, University of Western Ontario. Accessed January 15, 2021. https://ir.lib.uwo.ca/etd/480.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

To, Ciric Chi Wing. “Molecular Mechanisms of Cell Migration Inhibition by Synthetic Triterpenoids.” 2012. Web. 15 Jan 2021.

Vancouver:

To CCW. Molecular Mechanisms of Cell Migration Inhibition by Synthetic Triterpenoids. [Internet] [Thesis]. University of Western Ontario; 2012. [cited 2021 Jan 15]. Available from: https://ir.lib.uwo.ca/etd/480.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

To CCW. Molecular Mechanisms of Cell Migration Inhibition by Synthetic Triterpenoids. [Thesis]. University of Western Ontario; 2012. Available from: https://ir.lib.uwo.ca/etd/480

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Toronto

29. Wang, Andrew Yuanbo. Ca2+ Influx Through TRPV4 Regulates DDR1-dependent Collagen Remodeling.

Degree: 2020, University of Toronto

URL: http://hdl.handle.net/1807/103170

►

Tissue fibrosis involves excessive deposition of disorganized collagen fibrils, which interferes with organ structure and function. The Discoidin Domain Receptor 1 (DDR1), a fibrillar collagen… (more)

Subjects/Keywords: Adhesion receptor; Calcium; Collagen remodeling; Fibrosis; Focal adhesions; Myosin; 0379

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APA (6^th Edition):

Wang, A. Y. (2020). Ca2+ Influx Through TRPV4 Regulates DDR1-dependent Collagen Remodeling. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/103170

Chicago Manual of Style (16^th Edition):

Wang, Andrew Yuanbo. “Ca2+ Influx Through TRPV4 Regulates DDR1-dependent Collagen Remodeling.” 2020. Masters Thesis, University of Toronto. Accessed January 15, 2021. http://hdl.handle.net/1807/103170.

MLA Handbook (7^th Edition):

Wang, Andrew Yuanbo. “Ca2+ Influx Through TRPV4 Regulates DDR1-dependent Collagen Remodeling.” 2020. Web. 15 Jan 2021.

Vancouver:

Wang AY. Ca2+ Influx Through TRPV4 Regulates DDR1-dependent Collagen Remodeling. [Internet] [Masters thesis]. University of Toronto; 2020. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/1807/103170.

Council of Science Editors:

Wang AY. Ca2+ Influx Through TRPV4 Regulates DDR1-dependent Collagen Remodeling. [Masters Thesis]. University of Toronto; 2020. Available from: http://hdl.handle.net/1807/103170

University of Toronto

30. Behman, Ramy. The Management of Adhesive Small Bowel Obstruction: A Population-based Analysis of Practices and Outcomes.

Degree: PhD, 2019, University of Toronto

URL: http://hdl.handle.net/1807/99901

►

Background Adhesive small bowel obstruction (aSBO) is a common surgical illness that is characterized by its high likelihood for recurrence. We sought to compare different… (more)

Subjects/Keywords: Adhesions; Clinical Epidemiology; Health Care Research; Small Bowel Obstruction; 0766

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APA (6^th Edition):

Behman, R. (2019). The Management of Adhesive Small Bowel Obstruction: A Population-based Analysis of Practices and Outcomes. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/99901

Chicago Manual of Style (16^th Edition):

Behman, Ramy. “The Management of Adhesive Small Bowel Obstruction: A Population-based Analysis of Practices and Outcomes.” 2019. Doctoral Dissertation, University of Toronto. Accessed January 15, 2021. http://hdl.handle.net/1807/99901.

MLA Handbook (7^th Edition):

Behman, Ramy. “The Management of Adhesive Small Bowel Obstruction: A Population-based Analysis of Practices and Outcomes.” 2019. Web. 15 Jan 2021.

Vancouver:

Behman R. The Management of Adhesive Small Bowel Obstruction: A Population-based Analysis of Practices and Outcomes. [Internet] [Doctoral dissertation]. University of Toronto; 2019. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/1807/99901.

Council of Science Editors:

Behman R. The Management of Adhesive Small Bowel Obstruction: A Population-based Analysis of Practices and Outcomes. [Doctoral Dissertation]. University of Toronto; 2019. Available from: http://hdl.handle.net/1807/99901

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Open Access Theses and Dissertations