You searched for subject:(Adhesion).
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Oregon State University
1.
Saini, Gaurav.
Bacterial hydrophobicity : assessment techniques, applications and extension to colloids.
Degree: PhD, Chemical Engineering, 2010, Oregon State University
URL: http://hdl.handle.net/1957/16170 
► This research details laboratory assessment of bacterial hydrophobicity, variations in bacterial adhesion properties with growth and extended starvation as well as extension of this assay…
(more)
▼ This research details laboratory assessment of bacterial hydrophobicity, variations in bacterial
adhesion properties with growth and extended starvation as well as extension of this assay to colloidal hydrophobicity measurement. Hydrophobicity is an integral component of surface interactions and has been studied extensively for its role in a number of engineering and applied fields. A simple-to-do and quick experimental technique known as the Microbial
Adhesion to Hydrocarbons (MATH) test is the focus of this work. This method is based on determination of microbial hydrophobicity by differential partitioning at an aqueous-hydrocarbon interface and the results yield the hydrocarbon interaction affinity of the microbes. Though very popular, this assay still suffers from the lack of a standard protocol. As a first step in that direction, the effects of various operational parameters on MATH hydrophobicity measurements were studied. Some of the previously unexplored parameters like, absorbance wavelength, hydrocarbon saturation of aqueous media and suspension medium were found to affect the results. Application of a high concentration of a lyotrope, ammonium sulfate, was shown to enhance the MATH hydrophobicity of bacteria. Additionally, cell size measurements revealed that the affinity of sulfate ions for water molecules is the primary cause of this increase rather than cell agglomeration. Increased hydrophobicity can be beneficially employed for oral care, prevention of urinary tract infection and mixed microbial community analysis.
Bacteria are often subjected to nutrient variations in different environments which significantly alter their
adhesion to surfaces thereby affecting biofilm development and have an important bearing on biofouling. An in-depth study of variations in some of the more common
adhesion determinants (biomass, cell size and hydrophobicity) demonstrated a decrease in hydrophobicity from log-growth to stationary growth phases. Short term starvation (up to 7 days) led to significant variations in measured parameters. Starved cells were also more susceptible to hydrocarbon exposure and exhibited smaller cell sizes than growth cultures.
Colloids, the larger particle family of which bacteria are a sub-class, lack a simple assay for hydrophobicity measurement. The MATH test was successfully extended to colloidal domain (non-biological particles) and the hydrophobicity results were verified against the more traditional method- water contact angle measurement. We observed similar hydrophobicity trends, as measured by the MATH test and contact angle measurements, for most of the colloids tested in this work. This dissertation research is expected to enhance our understanding of hydrophobicity in the context of bacteria and colloids, through simple experiments, and reinforce our knowledge of the dynamic nature of bacteria.
Advisors/Committee Members: Wood, Brian D. (advisor), Dolan, Mark E. (committee member).
Subjects/Keywords: Bacterial Adhesion; Bacteria – Adhesion
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APA (6th Edition):
Saini, G. (2010). Bacterial hydrophobicity : assessment techniques, applications and extension to colloids. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/16170
Chicago Manual of Style (16th Edition):
Saini, Gaurav. “Bacterial hydrophobicity : assessment techniques, applications and extension to colloids.” 2010. Doctoral Dissertation, Oregon State University. Accessed March 01, 2021.
http://hdl.handle.net/1957/16170.
MLA Handbook (7th Edition):
Saini, Gaurav. “Bacterial hydrophobicity : assessment techniques, applications and extension to colloids.” 2010. Web. 01 Mar 2021.
Vancouver:
Saini G. Bacterial hydrophobicity : assessment techniques, applications and extension to colloids. [Internet] [Doctoral dissertation]. Oregon State University; 2010. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/1957/16170.
Council of Science Editors:
Saini G. Bacterial hydrophobicity : assessment techniques, applications and extension to colloids. [Doctoral Dissertation]. Oregon State University; 2010. Available from: http://hdl.handle.net/1957/16170
2.
Scott, Gunner.
Controllable Dry Adhesion due to Surface Micro-Defect Patterning.
Degree: 2019, University of Nevada – Reno
URL: http://hdl.handle.net/11714/6657
► Robotic automation continues to develop and is constantly in need for new gripping devices. Devices are needed to handle small delicate objects for pick and…
(more)
▼ Robotic automation continues to develop and is constantly in need for new gripping devices. Devices are needed to handle small delicate objects for pick and place applications. Traditional rigid grippers have difficulty adapting to varying objects, and pneumatic grippers require bulky pumps and compressed air. Dry
adhesion devices can remove the need for rigid components or pneumatics by relying on
adhesion as the main method to pick up and manipulate objects. Tunable dry
adhesion refers to the ability of controlling
adhesion, increasing and decreasing it as the task requires. Research continues to develop devices that offer the highest tunability ratio. Based on a subsurface stiffness modulating device proposed by Shan et al. a novel method for increase
adhesion in the normal direction is proposed, using circular defects on the adhering surface of a PDMS (polydimethysiloxane) post. The fabrication process included photolithography and molding which produced high resolution defects with high placement accuracy. The proposed patterns resulted in an increased
adhesion, and for certain patterns, an increase of roughly 40%, compared to a flat PDMS post. Simulations helped explain the point of failure observed in the experimental results and provided some clarification to the increase of
adhesion. Contact splitting and other mechanics offer explanations for the increase of
adhesion and work continues to be done to further identify the mechanics involved. Defect patterning can be paired with subsurface stiffness modulation, previously studied by the Shan Research Group to increase the tunability ratio and develop new devices for pick and place automation.
Advisors/Committee Members: Fan, Feifei (advisor), Kirn, Adam (committee member), Van Breugel, Floris (committee member).
Subjects/Keywords: Defect; Dry Adhesion; Tunable Adhesion
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APA (6th Edition):
Scott, G. (2019). Controllable Dry Adhesion due to Surface Micro-Defect Patterning. (Thesis). University of Nevada – Reno. Retrieved from http://hdl.handle.net/11714/6657
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Scott, Gunner. “Controllable Dry Adhesion due to Surface Micro-Defect Patterning.” 2019. Thesis, University of Nevada – Reno. Accessed March 01, 2021.
http://hdl.handle.net/11714/6657.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Scott, Gunner. “Controllable Dry Adhesion due to Surface Micro-Defect Patterning.” 2019. Web. 01 Mar 2021.
Vancouver:
Scott G. Controllable Dry Adhesion due to Surface Micro-Defect Patterning. [Internet] [Thesis]. University of Nevada – Reno; 2019. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/11714/6657.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Scott G. Controllable Dry Adhesion due to Surface Micro-Defect Patterning. [Thesis]. University of Nevada – Reno; 2019. Available from: http://hdl.handle.net/11714/6657
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
3.
Webler, Forrest.
Quantifying the Adhesion Surface Preference of the
Caulobacter crescentus Holdfast.
Degree: Biomedical Engineering, 2017, Brown University
URL: https://repository.library.brown.edu/studio/item/bdr:733559/
► Caulobacter crescentus is a gram-negative, non-pathogenic, freshwater bacterium which has recently captured the attention of researches in the multidisciplinary study of bioadhesives. C. crescentus is…
(more)
▼ Caulobacter crescentus is a gram-negative,
non-pathogenic, freshwater bacterium which has recently captured
the attention of researches in the multidisciplinary study of
bioadhesives. C. crescentus is unique in that it has evolved the
ability to adhere to surfaces with the unparalleled strength,
equivalent to a force per area of 68N/mm2, via a gel-like epoxy
secretion known as holdfast. Although the holdfast of C. crescentus
rivals all currently available adhesives, it is not yet possible to
produce a biologically-derived glue from the holdfast. Molecular
biologists have yet to uncover the complex protein structure of the
holdfast. To better understand and elucidate properties of the
Caulobacter crescentus holdfast we quantify cell density as a
function of time to determine if differences in material properties
effect the binding affinity of C. crescentus. This thesis also
presents multiple methodologies for quantifying
adhesion rates on
different substrates. Multiple assays with wild-type C. crescentus
cells indicate an average of 16.11% more surface coverage on
borosilicate glass surfaces than untreated polystyrene if cells are
incubated on the two materials for a given time ∆t. We believe that
these results are due to the surface charge profile of the
material. Slightly charged amines in the holdfast are attracted to
the more negatively charged glass leading to increased
adhesion
events whereas polystyrene is a nonpolar substrate providing less
of an electrostatic, attraction.
Advisors/Committee Members: Shukla, Anita (Reader), Pelcovits, Robert (Reader), Tang, Jay (Advisor).
Subjects/Keywords: Cell adhesion
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APA (6th Edition):
Webler, F. (2017). Quantifying the Adhesion Surface Preference of the
Caulobacter crescentus Holdfast. (Thesis). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:733559/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Webler, Forrest. “Quantifying the Adhesion Surface Preference of the
Caulobacter crescentus Holdfast.” 2017. Thesis, Brown University. Accessed March 01, 2021.
https://repository.library.brown.edu/studio/item/bdr:733559/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Webler, Forrest. “Quantifying the Adhesion Surface Preference of the
Caulobacter crescentus Holdfast.” 2017. Web. 01 Mar 2021.
Vancouver:
Webler F. Quantifying the Adhesion Surface Preference of the
Caulobacter crescentus Holdfast. [Internet] [Thesis]. Brown University; 2017. [cited 2021 Mar 01].
Available from: https://repository.library.brown.edu/studio/item/bdr:733559/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Webler F. Quantifying the Adhesion Surface Preference of the
Caulobacter crescentus Holdfast. [Thesis]. Brown University; 2017. Available from: https://repository.library.brown.edu/studio/item/bdr:733559/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
4.
Qian, Jin.
Mechanics of Molecular Bond Clusters between Elastic Media:
Stochastic-elastic Coupling in Cell-matrix Adhesion.
Degree: PhD, Division of Engineering. Mechanics of
Solids, 2009, Brown University
URL: https://repository.library.brown.edu/studio/item/bdr:211/
► Cell-matrix adhesion depends on the collective behavior of a large number of molecular bonds. While the behavior of a single molecular bond is governed by…
(more)
▼ Cell-matrix
adhesion depends on the collective
behavior of a large number of molecular bonds. While the behavior
of a single molecular bond is governed by statistical mechanics,
continuum mechanics should be valid at a large scale. How can this
transition be modeled and can this tell us something about the
mechanics of cell
adhesion? We consider in this thesis an idealized
theoretical model of molecular bond clusters between two dissimilar
elastic media subjected to various forms of mechanical load. The
distribution of interfacial traction is assumed to obey classical
elasticity equations while the rupture and rebinding of individual
molecular bonds are governed by stochastic equations. We show that
the interfacial traction is generally shared among the molecular
bonds in a non-uniform manner governed by the elasticity of the
system. Monte Carlo simulations that combine the elastic and
stochastic descriptions are conducted to investigate the lifetime
of molecular bond clusters as a function of the loading magnitude
for given
adhesion sizes. Generally, the average cluster lifetime
asymptotically approaches infinity as the applied load is reduced
to below a critical value, defined as the
adhesion strength. The
effects of cluster size, cell/matrix modulus, rebinding rate and
loading direction on the cluster lifetime and strength are
systematically investigated. While overly simplified in a number of
aspects, the model discussed in this thesis seems to give
predictions that are broadly consistent with relevant experimental
observations on cell-matrix
adhesion. The second topic of this
thesis deals with the capillary
adhesion in insects like beetles
and blowflies. We investigate the scaling effects of wet
adhesion
mediated by a liquid bridge between a fiber and a solid surface.
The influences of liquid volume and contact angles are discussed
via a scaling law indicating that the
adhesion strength can be
enhanced by contact size reduction. Due to the negative pressure in
the liquid bridge, there exists a critical length scale at which
the system achieves the theoretical tensile strength of the liquid.
We conclude that size reduction down to a critical scale results in
optimization of the
adhesion strength.
Advisors/Committee Members: Gao, Huajian (director), Freund, Lambert (reader), Franck, Christian (reader).
Subjects/Keywords: Focal Adhesion
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APA ·
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CSE |
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Manager
APA (6th Edition):
Qian, J. (2009). Mechanics of Molecular Bond Clusters between Elastic Media:
Stochastic-elastic Coupling in Cell-matrix Adhesion. (Doctoral Dissertation). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:211/
Chicago Manual of Style (16th Edition):
Qian, Jin. “Mechanics of Molecular Bond Clusters between Elastic Media:
Stochastic-elastic Coupling in Cell-matrix Adhesion.” 2009. Doctoral Dissertation, Brown University. Accessed March 01, 2021.
https://repository.library.brown.edu/studio/item/bdr:211/.
MLA Handbook (7th Edition):
Qian, Jin. “Mechanics of Molecular Bond Clusters between Elastic Media:
Stochastic-elastic Coupling in Cell-matrix Adhesion.” 2009. Web. 01 Mar 2021.
Vancouver:
Qian J. Mechanics of Molecular Bond Clusters between Elastic Media:
Stochastic-elastic Coupling in Cell-matrix Adhesion. [Internet] [Doctoral dissertation]. Brown University; 2009. [cited 2021 Mar 01].
Available from: https://repository.library.brown.edu/studio/item/bdr:211/.
Council of Science Editors:
Qian J. Mechanics of Molecular Bond Clusters between Elastic Media:
Stochastic-elastic Coupling in Cell-matrix Adhesion. [Doctoral Dissertation]. Brown University; 2009. Available from: https://repository.library.brown.edu/studio/item/bdr:211/
University of Illinois – Urbana-Champaign
5.
Rahil, Zainab.
Cellular decision making at the nanoscale.
Degree: MS, Bioengineering, 2016, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/98323
► The well-established dependence of cell traction forces on the compliance of supporting matrices has been attributed to levels of force exerted on components in focal…
(more)
▼ The well-established dependence of cell traction forces on the compliance of supporting matrices has been attributed to levels of force exerted on components in focal contacts. Here, use of novel, force-limited nanoscale tension gauges revealed that both force and substrate deformations govern cell decision-making during initial attachment to compliant substrates. We propose a mechanical model consistent with observed behavior. Upon formation of stable cell contacts, bond tension and tether rupture govern cell attachment, spreading, and focal
adhesion maturation at force levels on individual receptors predicted by prior studies.
Advisors/Committee Members: Leckband, Deborah (advisor), Ha, Taekjip (committee member).
Subjects/Keywords: Cell adhesion
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APA (6th Edition):
Rahil, Z. (2016). Cellular decision making at the nanoscale. (Thesis). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/98323
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Rahil, Zainab. “Cellular decision making at the nanoscale.” 2016. Thesis, University of Illinois – Urbana-Champaign. Accessed March 01, 2021.
http://hdl.handle.net/2142/98323.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Rahil, Zainab. “Cellular decision making at the nanoscale.” 2016. Web. 01 Mar 2021.
Vancouver:
Rahil Z. Cellular decision making at the nanoscale. [Internet] [Thesis]. University of Illinois – Urbana-Champaign; 2016. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/2142/98323.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Rahil Z. Cellular decision making at the nanoscale. [Thesis]. University of Illinois – Urbana-Champaign; 2016. Available from: http://hdl.handle.net/2142/98323
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Georgia Tech
6.
Gomez, Ismael J.
Utilizing pollen as a bio-organic template for tailorable multimodal adhesion.
Degree: PhD, Chemical and Biomolecular Engineering, 2013, Georgia Tech
URL: http://hdl.handle.net/1853/52963
► The interactions of microparticles, particularly those possessing nano- and microstructured asperities, play a critical role in many industrial applications. As a result, control over particle-particle…
(more)
▼ The interactions of microparticles, particularly those possessing nano- and microstructured asperities, play a critical role in many industrial applications. As a result, control over particle-particle and particle-surface interactions can be accomplished by designing microparticles with well-defined surface morphologies. Nature provides remarkable examples of evolutionary-optimized microscale biological particles with structures and/or chemistries tailored for effective
adhesion to a variety of surfaces under different dynamic and environmental conditions. Prominent among these are pollen, which possess a range of ornamentations consisting of combinations of various morphologies and feature sizes. These surface structures, provided by a highly chemically and mechanically stable outer shell, make pollen a model bioparticle for evaluating geometric effects on
adhesion.
This research aims to take advantage of pollen's unique architecture by utilizing it as a biotemplate for designing pollen-derived particles with tailorable microparticle
adhesion. In this work, the
adhesion behavior of pollen and pollen-derived particles is characterized using atomic force microscopy (AFM). Cleaned natural pollen particles were found to exhibit short-range van der Waals (VDW)
adhesion strengths that were independent of surface chemistry and scaled with the tip radius of pollen's ornamentations. Employing pollen as a core material, electrostatic interactions were utilized to controllably coat metal nanoparticles onto pollen's surface. Metal nanoparticle-coated pollen particles displayed enhanced
adhesion facilitated by multiple nanoparticle contacts with probe surfaces, while also showing potential for use as surface enhanced Raman scattering (SERS) substrates. Using pollen as a template, a layer-by-layer (LbL) surface sol-gel (SSG) technique allowed for the preparation of high-fidelity ferro- and ferrimagnetic replicas exhibiting short-range VDW-based
adhesion governed by the contact of nanocrystals present, and long-range magnetic attraction governed by the magnetic properties of ferrimagnetic pollen replicas. The results of this work highlight the feasibility of utilizing pollen as a bio-organic template and the potential for designing pollen-derived particles with tailorable
adhesion.
Advisors/Committee Members: Meredith, Carson (advisor), Beckham, Haskell W. (committee member), Behrens, Sven (committee member), Deng, Yulin (committee member), Thio, Yonathan (committee member).
Subjects/Keywords: Pollen; Adhesion
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APA (6th Edition):
Gomez, I. J. (2013). Utilizing pollen as a bio-organic template for tailorable multimodal adhesion. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/52963
Chicago Manual of Style (16th Edition):
Gomez, Ismael J. “Utilizing pollen as a bio-organic template for tailorable multimodal adhesion.” 2013. Doctoral Dissertation, Georgia Tech. Accessed March 01, 2021.
http://hdl.handle.net/1853/52963.
MLA Handbook (7th Edition):
Gomez, Ismael J. “Utilizing pollen as a bio-organic template for tailorable multimodal adhesion.” 2013. Web. 01 Mar 2021.
Vancouver:
Gomez IJ. Utilizing pollen as a bio-organic template for tailorable multimodal adhesion. [Internet] [Doctoral dissertation]. Georgia Tech; 2013. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/1853/52963.
Council of Science Editors:
Gomez IJ. Utilizing pollen as a bio-organic template for tailorable multimodal adhesion. [Doctoral Dissertation]. Georgia Tech; 2013. Available from: http://hdl.handle.net/1853/52963
McGill University
7.
Couture, Clement.
The gp23/45/90 thymic adhesion complex.
Degree: PhD, Department of Experimental Medicine, 1992, McGill University
URL: https://escholarship.mcgill.ca/downloads/ht24wm54f.pdf
;
https://escholarship.mcgill.ca/concern/theses/0r967607n
► Il est présentement reconnu que les interactions lympho-stromales jouent un rôle vital dans la régulation de tous les évènements associes au développement des lymphocytes T…
(more)
▼ Il est présentement reconnu que les interactions lympho-stromales jouent un rôle vital dans la régulation de tous les évènements associes au développement des lymphocytes T dans le thymus. En conséquence, les molécules impliqués dans ces interactions, de même que les signaux intracellulaires induits chez les thymocytes lors de ces interactions avec les éléments stromaux du thymus, font l'objet d'une intense investigation. Un modèle d'interaction lympho-stromale est l'adhérence des thymocytes CD4+CD8+ aux cellules épithéliale médullaire thymiques de la lignée E-5. Dans ce travail, nous avons développé un test d'adhérence en phase solide pour démontrer que l'adhérence des thymocytes CD4+CD8+ aux cellules E-5 est médiée par une nouvelle molécule d'adhérence, le dimère gp23/45, qui est exprimé par les cellules épithéliales. […]
Lympho-stromal interactions have been shown to play a vital role in the control of all aspects of intrathymic T cell development. As a consequence, the molecules involved in these interactions and the intracellular signals triggered in developing T cells during their interactions with the stromal elements of the thymus have become the subject of intense investigation. One model of such lympho-stromal interaction is the adhesion of CD4+cos+ thymocytes to the E-5 thymic medullary epithelial cell line. In this work, we developed a solid-phase adhesion assay to demonstrate that the interactionof C04+cos+ thymocytes with E-5 cells is mediated by a novel adhesion molecule, the gp23/45 complex, expressed by the epithelial cells. […]
Advisors/Committee Members: Potworowski, Edouard F. (Supervisor).
Subjects/Keywords: Adhesion
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APA (6th Edition):
Couture, C. (1992). The gp23/45/90 thymic adhesion complex. (Doctoral Dissertation). McGill University. Retrieved from https://escholarship.mcgill.ca/downloads/ht24wm54f.pdf ; https://escholarship.mcgill.ca/concern/theses/0r967607n
Chicago Manual of Style (16th Edition):
Couture, Clement. “The gp23/45/90 thymic adhesion complex.” 1992. Doctoral Dissertation, McGill University. Accessed March 01, 2021.
https://escholarship.mcgill.ca/downloads/ht24wm54f.pdf ; https://escholarship.mcgill.ca/concern/theses/0r967607n.
MLA Handbook (7th Edition):
Couture, Clement. “The gp23/45/90 thymic adhesion complex.” 1992. Web. 01 Mar 2021.
Vancouver:
Couture C. The gp23/45/90 thymic adhesion complex. [Internet] [Doctoral dissertation]. McGill University; 1992. [cited 2021 Mar 01].
Available from: https://escholarship.mcgill.ca/downloads/ht24wm54f.pdf ; https://escholarship.mcgill.ca/concern/theses/0r967607n.
Council of Science Editors:
Couture C. The gp23/45/90 thymic adhesion complex. [Doctoral Dissertation]. McGill University; 1992. Available from: https://escholarship.mcgill.ca/downloads/ht24wm54f.pdf ; https://escholarship.mcgill.ca/concern/theses/0r967607n
Rutgers University
8.
Anand, Prachi S.
Surface modification of biodegradable and biocompatible polymer scaffolds with multifunctional self-assembled monolayers for the controlled and specific adhesion of the biomolecules.
Degree: MS, Chemistry, 2015, Rutgers University
URL: https://rucore.libraries.rutgers.edu/rutgers-lib/48768/
► Dynamic surfaces can be customized to form a multifunctional assembly that favors the adsorption of the proteins and the sugar molecules in a one pot…
(more)
▼ Dynamic surfaces can be customized to form a multifunctional assembly that favors the adsorption of the proteins and the sugar molecules in a one pot reaction and provides a control over the orientation and conformation of the bioactive ligands on the synthetic surface. Self-assembled monolayers SAMs), bearing bifunctional groups grant a straightforward, flexible and simplistic method to overcome the limitations posed by a biopolymer-peptide system. These bifunctional moieties on one terminus can be adhered to the synthetic biopolymer that acts as a scaffold for the assembly and on the other terminus can be tailored to attach to highly specific biomolecules such as peptides and carbohydrates . In a novel synthesis, the SAMs can be attached to the different biomolecules by multicomponent reactions (MCR). MCRs are the chemical reactions in which three or more reactants form a product. Although, these reactions have been known for a long time, but have yet to be used in the synthesis of the biological components that mimic the extracellular matrix (ECM).
Advisors/Committee Members: Sheridan, John (chair), Jordan, Frank (internal member), Huskey, Phil (internal member).
Subjects/Keywords: Biomolecules; Adhesion
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APA (6th Edition):
Anand, P. S. (2015). Surface modification of biodegradable and biocompatible polymer scaffolds with multifunctional self-assembled monolayers for the controlled and specific adhesion of the biomolecules. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/48768/
Chicago Manual of Style (16th Edition):
Anand, Prachi S. “Surface modification of biodegradable and biocompatible polymer scaffolds with multifunctional self-assembled monolayers for the controlled and specific adhesion of the biomolecules.” 2015. Masters Thesis, Rutgers University. Accessed March 01, 2021.
https://rucore.libraries.rutgers.edu/rutgers-lib/48768/.
MLA Handbook (7th Edition):
Anand, Prachi S. “Surface modification of biodegradable and biocompatible polymer scaffolds with multifunctional self-assembled monolayers for the controlled and specific adhesion of the biomolecules.” 2015. Web. 01 Mar 2021.
Vancouver:
Anand PS. Surface modification of biodegradable and biocompatible polymer scaffolds with multifunctional self-assembled monolayers for the controlled and specific adhesion of the biomolecules. [Internet] [Masters thesis]. Rutgers University; 2015. [cited 2021 Mar 01].
Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/48768/.
Council of Science Editors:
Anand PS. Surface modification of biodegradable and biocompatible polymer scaffolds with multifunctional self-assembled monolayers for the controlled and specific adhesion of the biomolecules. [Masters Thesis]. Rutgers University; 2015. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/48768/
University of Adelaide
9.
Lauder, Christopher Ian William.
Peritoneal adhesion formation and modulation.
Degree: 2011, University of Adelaide
URL: http://hdl.handle.net/2440/69662
► This thesis examines the subject of peritoneal adhesion formation following surgery in the format of a Master of Surgery by publication. A comprehensive literature review…
(more)
▼ This thesis examines the
subject of peritoneal
adhesion formation following surgery in the format of a Master of Surgery by publication. A comprehensive literature review examines all aspects of peritoneal adhesions from the basic science to the evidence supporting products aimed at
adhesion reduction. Subsequent experimental work utilises two animal models to form adhesions and test the ability of a novel gel product to reduce
adhesion formation. The gel is a hybrid hydrogel consisting of modified chitosan and dextran. These two components are combined by a chemical cross linking reaction to form an inert gel that can be applied to the site of surgery. The gel confers several beneficial properties when used to prevent adhesions. Firstly it provides a physical separation of the injured peritoneal surfaces whilst also inhibiting the ingress of fibroblasts to the area. Secondary characteristics which promote haemostasis and inhibit bacterial growth enhance the gels
adhesion reducing potential. Initially the gel was trialled in a small animal model to test varying compositions and volumes of the gel. Two different surgical models of
adhesion formation were utilised to provide a range of stimuli in the post operative period. Results from these experiments were encouraging, showing a statistically significant reduction in
adhesion formation. Following on from this initial study a large animal study was conceived to further evaluate the effectiveness of the gel in differing environments. The porcine model also allowed for a true bowel resection with anastomosis to test the safety of the gel when used in this scenario. Allied to this the gel was also trialled following adhesiolysis at the mid point of the study, while monitoring for sensitisation or toxicity to the gel. Infective complications and abscess formation proved to be a difficult hurdle to overcome in this model. As such, no significant reduction in
adhesion reformation following adhesiolysis was observed. There were however some promising results with a reduction in adhesions to the wound noted with treatment at the time of laparoscopy, as well as a reduction in adhesions involving the bowel at the study end point. The experimental work highlights the difficulties associated with peritoneal
adhesion reduction. Overcoming the numerous stimuli to
adhesion formation is not an easy task, and there remains no currently available treatment for the prevention of adhesions without certain caveats to its use. An effective product that could be used safely in practically all environments would certainly be a step forward in this branch of surgical research. It is plausible that a product such as the gel may be improved upon to show further benefit. However, long term studies will still be required to show a beneficial effect in long term outcome measures such as the incidence of small bowel obstruction.
Advisors/Committee Members: Maddern, Guy John (advisor), School of Medicine (school).
Subjects/Keywords: peritoneal adhesions; adhesion prevention; adhesion treatment
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APA (6th Edition):
Lauder, C. I. W. (2011). Peritoneal adhesion formation and modulation. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/69662
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Lauder, Christopher Ian William. “Peritoneal adhesion formation and modulation.” 2011. Thesis, University of Adelaide. Accessed March 01, 2021.
http://hdl.handle.net/2440/69662.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Lauder, Christopher Ian William. “Peritoneal adhesion formation and modulation.” 2011. Web. 01 Mar 2021.
Vancouver:
Lauder CIW. Peritoneal adhesion formation and modulation. [Internet] [Thesis]. University of Adelaide; 2011. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/2440/69662.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Lauder CIW. Peritoneal adhesion formation and modulation. [Thesis]. University of Adelaide; 2011. Available from: http://hdl.handle.net/2440/69662
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Cambridge
10.
Redmann, Anna-Lena.
Kinetics of cell attachment and spreading on hard and soft substrates.
Degree: PhD, 2019, University of Cambridge
URL: https://www.repository.cam.ac.uk/handle/1810/290385
► A very important aspect for the functioning of an organism is that cells adapt their behaviour to external stimuli. They continuously interact with their environment,…
(more)
▼ A very important aspect for the functioning of an organism is that cells adapt their behaviour to external stimuli. They continuously interact with their environment, and biochemical and physical cues can activate cellular signalling, which leads to changes in cell behaviour such as proliferation and shape. Understanding cells' interactions with their environment is also important for understanding diseases. For example mechanosensing, which is the sensing of the cell's mechanical environment, has been associated with cancer development. In order for a cell to be able to sense its mechanical environment, it needs to form attachments to the environment.
In my thesis, I have worked on three different tasks: the development of a new measurement technique and the study of initial cell adhesion and of cell spreading. When a cell from suspension first comes into contact with a substrate, it forms initial attachment bonds with proteins on the substrate surface. These bonds are mediated through integrins, which are transmembrane heterodimers, binding to the cell's environment on one side and to the cell's cytoskeleton on the other side.
I study this initial cell attachment by measuring the force needed to detach cells, called cell adhesion strength. For these experiments I built a detachment device, which allows the detachment of cells from a substrate by vibrating the substrate in liquid. The device combines cell incubation, detachment and imaging. I measured the dependence of initial integrin bond formation on external factors such as incubation temperature and substrate stiffness.
Once initial integrin bonds are formed, many different proteins are recruited to the adhesion site in order to form stronger adhesions. Amongst these proteins are signalling proteins, which direct the behaviour of the cell as a whole. One of the first cellular reactions to a substrate after initial integrin binding is cell spreading. This can be seen by the cell changing its shape from spherical to dome-like on the substrate. Because cell spreading is a very early response of a cell to a substrate, the onset time of spreading can be used as a quantitative measure for the time it takes the cell to sense a substrate and signal shape change.
In my work, I look at the distribution of the time of initial cell spreading in a population of cells. I measure this distribution under different growth conditions such as pH, change of incubation medium from DMEM to PBS, substrate stiffness and incubation temperature.
In my detachment experiments, I observe that vibration accelerates cell spreading in those cells which remain on the substrate. This is a connection between the detachment experiments and the cell spreading experiments and it shows how cells react to external forces. By changing the medium temperature in the cell detachment and cell spreading experiments, I am able to analyse the kinetics of these two processes. I use a signalling network model to analyse the internal cellular signalling path that leads from a spherical to a spread cell.
Subjects/Keywords: Cell adhesion; Cell spreading; Adhesion strength assay
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APA (6th Edition):
Redmann, A. (2019). Kinetics of cell attachment and spreading on hard and soft substrates. (Doctoral Dissertation). University of Cambridge. Retrieved from https://www.repository.cam.ac.uk/handle/1810/290385
Chicago Manual of Style (16th Edition):
Redmann, Anna-Lena. “Kinetics of cell attachment and spreading on hard and soft substrates.” 2019. Doctoral Dissertation, University of Cambridge. Accessed March 01, 2021.
https://www.repository.cam.ac.uk/handle/1810/290385.
MLA Handbook (7th Edition):
Redmann, Anna-Lena. “Kinetics of cell attachment and spreading on hard and soft substrates.” 2019. Web. 01 Mar 2021.
Vancouver:
Redmann A. Kinetics of cell attachment and spreading on hard and soft substrates. [Internet] [Doctoral dissertation]. University of Cambridge; 2019. [cited 2021 Mar 01].
Available from: https://www.repository.cam.ac.uk/handle/1810/290385.
Council of Science Editors:
Redmann A. Kinetics of cell attachment and spreading on hard and soft substrates. [Doctoral Dissertation]. University of Cambridge; 2019. Available from: https://www.repository.cam.ac.uk/handle/1810/290385
Harvard University
11.
Yang, Jiawei.
Hydrogel Adhesion.
Degree: PhD, 2019, Harvard University
URL: http://nrs.harvard.edu/urn-3:HUL.InstRepos:42106933
► Hydrogel adhesion, integrating hydrogels with a variety of materials, has advanced emerging technologies in the fields of functional materials, soft ionotronics and electronics, and biomedical…
(more)
▼ Hydrogel
adhesion, integrating hydrogels with a variety of materials, has advanced emerging technologies in the fields of functional materials, soft ionotronics and electronics, and biomedical applications. However, achieving strong
adhesion between hydrogels and other materials is fundamentally challenging. This thesis explores various ways to develop chemistry of bonds, mechanics of dissipation, and topology of connecting materials to create strong hydrogel
adhesion with diverse materials, and demonstrates potential applications in medicine and engineering.
In medical practices, strong
adhesion between hydrogel and biological tissues is important. Existing adhesives are either cytotoxic, adhere weakly to tissues, or cannot be used in wet environments. We design a bio-inspired adhesives consisting of two layers: an adhesive layer made of polymer chains and a dissipative layer made of a hydrogel capable of dissipation. The polymer chains can interpenetrate into both hydrogel and tissue, and form electrostatic interactions, covalent bonds, and physical interpenetration with the polymer networks of the hydrogel and the tissue. The two layers synergistically lead to higher
adhesion energy on various tissues than existing tissue adhesives.
Adhesion occurs within minutes, independent of blood exposure, and compatible with in vivo dynamic movements.
This class of tough adhesive is promising in tissue
adhesion, but the
adhesion restricts to specific functional groups from both materials. To address this issue, we develop a topological
adhesion that uses bio-compatible polymer chains to form a network, in topological entanglement with the two polymer networks of the hydrogel and the tissue, stitching them together like a suture at the molecular scale. This approach does not require functional groups from both materials, and the interface is mechanically compatible with soft hydrogels and tissues. To illustrate the principle, pH is used to trigger several polymers to form networks, and strong
adhesion can be created between hydrogels in full range of pH, and between hydrogels and tissues. The molecular suture can be further designed to be permanent, transient, or removable on-demand.
In developing the bonding methods so far, we realize the ways to achieve hydrogel
adhesion multiply considerably by examining another aspect of
adhesion: the topology of connecting materials. Topologies in existing methods are limited. Topologies of numerous varieties are possible, but have not been explored. To illustrate the potential, we delve into a specific bond-stitch topology, study its chemistry and physics, and highlight the synergy of chemistry, mechanics, and topology can enable strong
adhesion between any hydrogels and any materials. The diversity of topologies benefits the
adhesion to accommodate different materials and manufacturing processes, and also facilitates functional
adhesion for various purposes.
We study the mechanics of
adhesion. We conduct slow crack tests to identify the failure mechanism in the debonding of…
Advisors/Committee Members: Suo, Zhigang (advisor), Mooney, David J. (committee member), Vlassak, Joost J. (committee member), Hutchinsion, John W. (committee member), Rice, James R. (committee member).
Subjects/Keywords: Hydrogel; adhesion; chemistry; mechanics; topology; adhesion operations
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APA (6th Edition):
Yang, J. (2019). Hydrogel Adhesion. (Doctoral Dissertation). Harvard University. Retrieved from http://nrs.harvard.edu/urn-3:HUL.InstRepos:42106933
Chicago Manual of Style (16th Edition):
Yang, Jiawei. “Hydrogel Adhesion.” 2019. Doctoral Dissertation, Harvard University. Accessed March 01, 2021.
http://nrs.harvard.edu/urn-3:HUL.InstRepos:42106933.
MLA Handbook (7th Edition):
Yang, Jiawei. “Hydrogel Adhesion.” 2019. Web. 01 Mar 2021.
Vancouver:
Yang J. Hydrogel Adhesion. [Internet] [Doctoral dissertation]. Harvard University; 2019. [cited 2021 Mar 01].
Available from: http://nrs.harvard.edu/urn-3:HUL.InstRepos:42106933.
Council of Science Editors:
Yang J. Hydrogel Adhesion. [Doctoral Dissertation]. Harvard University; 2019. Available from: http://nrs.harvard.edu/urn-3:HUL.InstRepos:42106933
Delft University of Technology
12.
Cheyyar Nageswaran, P.D. (author).
Adhesion of aggregate-binder systems.
Degree: 2016, Delft University of Technology
URL: http://resolver.tudelft.nl/uuid:7e5631b8-4468-4f11-a9e6-f585209d94ad
► Adhesion is a difficult, but important phenomenon which needs to be addressed in the field of pavement engineering. A strong adhesion between the constituents of…
(more)
▼ Adhesion is a difficult, but important phenomenon which needs to be addressed in the field of pavement engineering. A strong adhesion between the constituents of pavement and between the layers of the pavement is an indication of its durability. Many modern materials like rubber, fibre, epoxy binder, adhesion promoter etc., are used as a replacement or addition to the conventional bitumen and aggregate materials in pavement construction. The introduction of these materials thus require a better understanding of their adhesive properties, which influences to a great extent, the pavement performance. Since there are no standard tests available to quantify adhesion in pavement engineering, the study aims at developing a framework of easily implementable test procedure that can be used for the assessment of the adhesive bond between the different materials used in pavement construction. This was achieved by performing direct tension test on different types of aggregate-binder systems. As the presence of moisture is a known factor that affects adhesion, in turn affecting performance of the pavement, the study also concentrated towards this aspect. Three types of aggregates (porphyry, diorite and sandstone), in combination with four different binders (bitumen pen70/100, SBS polymer modified bitumen, polyurethane based binder and epoxy based binder) and two adhesion promoters (Type a and b, with variation in curing time) were tested in this study. They were subjected to low and high temperature and strain rates and the effect of moisture on these materials were also investigated. It was found from this study that the direct tension test was easily implementable, which could help the contractors and researchers in quantifying the adhesion between the various aggregate-binder systems.
Road and Railroad Engineering
Structural Engineering
Civil Engineering and Geosciences
Advisors/Committee Members: Scarpas, A. (mentor).
Subjects/Keywords: adhesion; adhesion promoter; binder-aggregate interaction
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APA (6th Edition):
Cheyyar Nageswaran, P. D. (. (2016). Adhesion of aggregate-binder systems. (Masters Thesis). Delft University of Technology. Retrieved from http://resolver.tudelft.nl/uuid:7e5631b8-4468-4f11-a9e6-f585209d94ad
Chicago Manual of Style (16th Edition):
Cheyyar Nageswaran, P D (author). “Adhesion of aggregate-binder systems.” 2016. Masters Thesis, Delft University of Technology. Accessed March 01, 2021.
http://resolver.tudelft.nl/uuid:7e5631b8-4468-4f11-a9e6-f585209d94ad.
MLA Handbook (7th Edition):
Cheyyar Nageswaran, P D (author). “Adhesion of aggregate-binder systems.” 2016. Web. 01 Mar 2021.
Vancouver:
Cheyyar Nageswaran PD(. Adhesion of aggregate-binder systems. [Internet] [Masters thesis]. Delft University of Technology; 2016. [cited 2021 Mar 01].
Available from: http://resolver.tudelft.nl/uuid:7e5631b8-4468-4f11-a9e6-f585209d94ad.
Council of Science Editors:
Cheyyar Nageswaran PD(. Adhesion of aggregate-binder systems. [Masters Thesis]. Delft University of Technology; 2016. Available from: http://resolver.tudelft.nl/uuid:7e5631b8-4468-4f11-a9e6-f585209d94ad
Nanyang Technological University
13.
Cai, Ning.
Cell adhesion behavior on molecularly engineered surfaces
.
Degree: 2010, Nanyang Technological University
URL: http://hdl.handle.net/10356/47458
► Effective attachment of cells on biomaterials is one important requirement in designing engineered tissue substitute in application of tissue engineering. Engineering biomaterials surface with biomolecules…
(more)
▼ Effective attachment of cells on biomaterials is one important requirement in designing engineered tissue substitute in application of tissue engineering. Engineering biomaterials surface with biomolecules including ECM proteins is a widely-used method to enhance cell adhesion. The advancement in tissue engineering requires the design of optimized biomaterial surface to facilitate tissue regeneration, which relies on a better understanding of cell adhesion behavior on engineered biomaterial surfaces. In this work, poly(lactic acid) (PLA) and carbon nanotubes (CNT), chosen as the representative of conventional biomaterials and novel nano-biomaterials, respectively, were surface-modified with ECM proteins, collagen type I (COL) and fibronectin (FN), to improve their compatibility.
Subjects/Keywords: Cell adhesion behavior
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APA ·
Chicago ·
MLA ·
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Export
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Manager
APA (6th Edition):
Cai, N. (2010). Cell adhesion behavior on molecularly engineered surfaces
. (Thesis). Nanyang Technological University. Retrieved from http://hdl.handle.net/10356/47458
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Cai, Ning. “Cell adhesion behavior on molecularly engineered surfaces
.” 2010. Thesis, Nanyang Technological University. Accessed March 01, 2021.
http://hdl.handle.net/10356/47458.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Cai, Ning. “Cell adhesion behavior on molecularly engineered surfaces
.” 2010. Web. 01 Mar 2021.
Vancouver:
Cai N. Cell adhesion behavior on molecularly engineered surfaces
. [Internet] [Thesis]. Nanyang Technological University; 2010. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/10356/47458.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Cai N. Cell adhesion behavior on molecularly engineered surfaces
. [Thesis]. Nanyang Technological University; 2010. Available from: http://hdl.handle.net/10356/47458
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Cornell University
14.
Nadermann, Nichole.
Controllable Adhesion In A Bio-Inspired Film-Terminated Fibrillar Surface.
Degree: PhD, Mechanical Engineering, 2012, Cornell University
URL: http://hdl.handle.net/1813/31117
► In this dissertation we present work on the development, characterization, and analysis of an easy-to-fabricate, reusable bioinspired switchable adhesive that consists of a film-terminated fibrillar…
(more)
▼ In this dissertation we present work on the development, characterization, and analysis of an easy-to-fabricate, reusable bioinspired switchable adhesive that consists of a film-terminated fibrillar layer atop a substrate. We use a dynamic rod model and stability analysis to define and analyze the buckling of a fibril subjected to a prescribed shear displacement and a constant normal compressive force. Following this analysis, we present work on the switchable adhesive: a film-terminated fibrillar interface with two metastable states. In the first state, a thin film spanning the fibrillar surface results in strongly enhanced
adhesion due to crack-trapping. In the second state, the thin film collapses onto the substrate between fibrils and resembles a rough surface. We perform indentation experiments (pull-off and
adhesion hysteresis), which demonstrate differences in the adhesive response of the two states. We show that the adhesive state has a pull-off load up to 70 times higher than the non-adhesive state and has up to 20 times larger
adhesion hysteresis. Friction experiments show that in the collapsed state there is no static friction peak and that even in sliding friction, which is not enhanced for the adhesive state over the control samples (flat, unstructured PDMS), the collapsed state exhibits much lower sliding friction forces. We determine the pressure-to-collapse the thin film to switch from the adhesive to the non-adhesive state using hydrostatic pressure experiments. Finally, we perform both linear plate and von Kärman plate analyses on the thin film as it deforms under an applied pressure to gain insight into both bistability and the pressure required to collapse the thin film and in doing so, turn off the
adhesion. We find that the von Kärman plate theory more accurately captures the pressure required to initiate collapse of the thin film onto the substrate, most likely because of the large deflections taking place during collapse. To account for pressurization that occurs in our sealed samples during hydrostatic pressure experiments, we model the rate dependence in the hydrostatic pressure experiments. Pressurization and diffusion of gas through the thin film reduces the dependence on interfibrillar spacing.
Advisors/Committee Members: Hui, Chung-Yuen (chair), Mukherjee, Subrata (committee member), Gourdon, Delphine (committee member).
Subjects/Keywords: Bioinspired; Adhesion; Controllable
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APA (6th Edition):
Nadermann, N. (2012). Controllable Adhesion In A Bio-Inspired Film-Terminated Fibrillar Surface. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/31117
Chicago Manual of Style (16th Edition):
Nadermann, Nichole. “Controllable Adhesion In A Bio-Inspired Film-Terminated Fibrillar Surface.” 2012. Doctoral Dissertation, Cornell University. Accessed March 01, 2021.
http://hdl.handle.net/1813/31117.
MLA Handbook (7th Edition):
Nadermann, Nichole. “Controllable Adhesion In A Bio-Inspired Film-Terminated Fibrillar Surface.” 2012. Web. 01 Mar 2021.
Vancouver:
Nadermann N. Controllable Adhesion In A Bio-Inspired Film-Terminated Fibrillar Surface. [Internet] [Doctoral dissertation]. Cornell University; 2012. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/1813/31117.
Council of Science Editors:
Nadermann N. Controllable Adhesion In A Bio-Inspired Film-Terminated Fibrillar Surface. [Doctoral Dissertation]. Cornell University; 2012. Available from: http://hdl.handle.net/1813/31117
University of Cambridge
15.
Bullock, James Michael Rex.
Biomechanics of the fibrillar adhesive system in insects.
Degree: PhD, 2010, University of Cambridge
URL: http://www.dspace.cam.ac.uk/handle/1810/226862https://www.repository.cam.ac.uk/bitstream/1810/226862/2/license.txt
;
https://www.repository.cam.ac.uk/bitstream/1810/226862/5/Bullock%202010%20PhD%20Thesis.pdf.txt
;
https://www.repository.cam.ac.uk/bitstream/1810/226862/6/Bullock%202010%20PhD%20Thesis.pdf.jpg
► Many animals are able to scale smooth surfaces using adhesive structures on their feet. These organs are either soft pads with a relatively smooth surface…
(more)
▼ Many animals are able to scale smooth surfaces using adhesive structures on their feet. These organs are either soft pads with a relatively smooth surface or dense arrays of microscopic adhesive hairs with both designs having independently evolved in diverse taxa of arthropods and vertebrates. Biological adhesive pads out-perform conventional adhesives in many respects, making them important models for biomimetics. Hairy pads have attracted particular attention, because it has become feasible to fabricate similar synthetic microstructures. Nevertheless, the detailed performance and functional properties have not been characterised for any natural fibrillar adhesive system, and many fundamental aspects are still not understood. The aim of this thesis was therefore to investigate the fibrillar adhesive system of leaf beetles as a model.
To investigate the functional implications of hairy pad design, the attachment performance between hairy pads of the leaf beetle Gastrophysa viridula and smooth pads of stick insects (Carausius morosus) was compared. Adhesive and frictional stresses were found to be similar in smooth and hairy pads, inconsistent with contact splitting theory, which predicts higher adhesive stresses for fibrillar adhesives. Hairy pads showed a greater direction-dependence of friction forces than smooth pads, confirming the importance of the asymmetric design of individual setae for effortless detachment. Experiments with contaminating particles also showed that hairy pads removed contamination more rapidly and efficiently than smooth pads. Self-cleaning ability had not been previously documented for adhesive organs of insects. To investigate to what extent the hairy system is able to compensate for surface roughness, whole-body attachment forces were measured for varying roughness levels. Attachment was reduced for all length scales of surface roughness, but in particular for asperity sizes smaller than the diameter of individual seta tips.
Leaf beetles possess adhesive pads on three tarsal segments, which vary in setal morphology. However, the functional implications of this variation are unknown. The mechanical and adhesive properties of individual pads were therefore tested and their use during climbing observed. Proximal pads were shown to be stiffer than distal pads, conferring stability during pushing. In contrast, the softer distal pads allowed better attachment to rough surfaces. Hence the morphological variation is explained by an effective division of labour between the pads. To investigate an extreme example of pushing in a hairy system, pad use was studied during jumping in flea beetles. The pushing forces needed during take-off were exclusively produced by the proximal pads, again confirming the division of labour. To characterise the effects of different hair morphologies and to understand how individual setae contribute to array and whole-animal performance, single hair forces were measured using a glass capillary cantilever. Male-specific discoidal hairs were shown to be both stiffer…
Subjects/Keywords: Insect; Adhesion; Biomechanics
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APA (6th Edition):
Bullock, J. M. R. (2010). Biomechanics of the fibrillar adhesive system in insects. (Doctoral Dissertation). University of Cambridge. Retrieved from http://www.dspace.cam.ac.uk/handle/1810/226862https://www.repository.cam.ac.uk/bitstream/1810/226862/2/license.txt ; https://www.repository.cam.ac.uk/bitstream/1810/226862/5/Bullock%202010%20PhD%20Thesis.pdf.txt ; https://www.repository.cam.ac.uk/bitstream/1810/226862/6/Bullock%202010%20PhD%20Thesis.pdf.jpg
Chicago Manual of Style (16th Edition):
Bullock, James Michael Rex. “Biomechanics of the fibrillar adhesive system in insects.” 2010. Doctoral Dissertation, University of Cambridge. Accessed March 01, 2021.
http://www.dspace.cam.ac.uk/handle/1810/226862https://www.repository.cam.ac.uk/bitstream/1810/226862/2/license.txt ; https://www.repository.cam.ac.uk/bitstream/1810/226862/5/Bullock%202010%20PhD%20Thesis.pdf.txt ; https://www.repository.cam.ac.uk/bitstream/1810/226862/6/Bullock%202010%20PhD%20Thesis.pdf.jpg.
MLA Handbook (7th Edition):
Bullock, James Michael Rex. “Biomechanics of the fibrillar adhesive system in insects.” 2010. Web. 01 Mar 2021.
Vancouver:
Bullock JMR. Biomechanics of the fibrillar adhesive system in insects. [Internet] [Doctoral dissertation]. University of Cambridge; 2010. [cited 2021 Mar 01].
Available from: http://www.dspace.cam.ac.uk/handle/1810/226862https://www.repository.cam.ac.uk/bitstream/1810/226862/2/license.txt ; https://www.repository.cam.ac.uk/bitstream/1810/226862/5/Bullock%202010%20PhD%20Thesis.pdf.txt ; https://www.repository.cam.ac.uk/bitstream/1810/226862/6/Bullock%202010%20PhD%20Thesis.pdf.jpg.
Council of Science Editors:
Bullock JMR. Biomechanics of the fibrillar adhesive system in insects. [Doctoral Dissertation]. University of Cambridge; 2010. Available from: http://www.dspace.cam.ac.uk/handle/1810/226862https://www.repository.cam.ac.uk/bitstream/1810/226862/2/license.txt ; https://www.repository.cam.ac.uk/bitstream/1810/226862/5/Bullock%202010%20PhD%20Thesis.pdf.txt ; https://www.repository.cam.ac.uk/bitstream/1810/226862/6/Bullock%202010%20PhD%20Thesis.pdf.jpg
Georgia Tech
16.
Lee, Ted.
Triggerable ligand presentation using caged-RGD.
Degree: PhD, Mechanical Engineering, 2013, Georgia Tech
URL: http://hdl.handle.net/1853/52943
► Cells rely on time-dependent binding and activation by the ECM to initiate downstream signal transduction. It is unknown whether adhesion to a ligand is required…
(more)
▼ Cells rely on time-dependent binding and activation by the ECM to initiate downstream signal transduction. It is unknown whether
adhesion to a ligand is required throughout various cell processes, or only during a specified time period ("temporal threshold”). Current approaches to ligand presentation often comprise of static, constant densities of ligands. In contrast, natural cell adhesive interactions with ECMs exhibit spatiotemporal patterns of binding and activation. Therefore, a key to future research in controlling cell-material interactions will be the development of materials that can respond to external stimuli.
The objective of this project is to engineer biomaterials that present a UV-labile caged-Arginine-Glycine-Aspartic Acid (RGD) ligand and evaluate the effects on cell activities. RGD is the minimal adhesive sequence of fibronectin. By dynamically modulating adhesive ligand presentation, the effects of temporal control on cell processes can be elucidated. In this caged-peptide, a photo-labile group adjacent to the aspartic acid residue of RGD effectively “masks” a cyclo(RGDfk) peptide. Upon UV irradiation (360 nm), the caging group is released thereby restoring the adhesive activity of the peptide.
By having unparalleled spatiotemporal control of RGD ligand presentation, we demonstrated two novel tools for discovery: 1) in vivo ligand presentation to probe downstream tissue behavior and cell infiltration to biomaterial implants, and 2) in vitro ligand presentation in situ using confocal-based live cell microscopy to investigate real-time vinculin recruitment and cell traction force generation. These studies represented the first demonstration of triggerable adhesive ligand presentation in vivo and demonstrated the utility of caged-compounds for probing specific receptor-ligand responses on highly defined PEG-based hydrogels. Triggerable in vitro ligand presentation, combined with traction force microscopy, demonstrated a new research tool for investigating focal
adhesion formation and downstream force generation. Taken in whole, these results provide previously unknown insights into the importance of spatiotemporal control of adhesive ligands and created novel new research platforms for future discovery.
Advisors/Committee Members: García, Andrés (advisor), Barker, Thomas (committee member), Zhu, Cheng (committee member), Curtis, Jennifer (committee member).
Subjects/Keywords: Biomaterials; Cell adhesion
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APA (6th Edition):
Lee, T. (2013). Triggerable ligand presentation using caged-RGD. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/52943
Chicago Manual of Style (16th Edition):
Lee, Ted. “Triggerable ligand presentation using caged-RGD.” 2013. Doctoral Dissertation, Georgia Tech. Accessed March 01, 2021.
http://hdl.handle.net/1853/52943.
MLA Handbook (7th Edition):
Lee, Ted. “Triggerable ligand presentation using caged-RGD.” 2013. Web. 01 Mar 2021.
Vancouver:
Lee T. Triggerable ligand presentation using caged-RGD. [Internet] [Doctoral dissertation]. Georgia Tech; 2013. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/1853/52943.
Council of Science Editors:
Lee T. Triggerable ligand presentation using caged-RGD. [Doctoral Dissertation]. Georgia Tech; 2013. Available from: http://hdl.handle.net/1853/52943
Georgia Tech
17.
Shin, Donglee.
Effects of pollen and metal oxide pollen replica morphology on tailorable dry and wet adhesion.
Degree: PhD, Chemical and Biomolecular Engineering, 2017, Georgia Tech
URL: http://hdl.handle.net/1853/59776
► The surface adhesion of micro-particles has been investigated with respect to many natural and environmental phenomena as well as engineering applications including drug delivery, coating,…
(more)
▼ The surface
adhesion of micro-particles has been investigated with respect to many natural and environmental phenomena as well as engineering applications including drug delivery, coating, paints, separations, and sensors. Especially, the surface
adhesion of smooth micro-particles with simple shapes (spheres, cylinders, hexahedrons) has been well-studied for the last four or five decades. However, there are far fewer studies of the
adhesion of micro-particles with complex surface morphology. In this study, pollen grains are used as experimental samples and bio-templates since the pollen grains have chemically and physically stable surface with wide shape diversity. The purpose of this study is to understand the dry and wet adhesive mechanisms of pollen and pollen replica
adhesion to tailor the magnitude and rate-dependence of the
adhesion. During the investigation of the mechanisms, we have learned: i) how to analyze quantitatively and model the attraction force of micro-particles with complex surface micro- and nano-scale morphology ii) the effects of both particle morphology and liquid physical properties on wet
adhesion of micro-particles on surfaces iii) how to tune the rate-dependence of the wet
adhesion via control of surface morphology and the structure of liquid phases. Not only do these discoveries offer insight into the mechanisms operative in Nature, but they also offer inspiration for new
adhesion mechanisms for micro-particles, particularly those that are responsive to the dynamics of particle detachment speed.
Advisors/Committee Members: Meredith, J. Carson (advisor), Reichmanis, Elsa (committee member), Shofner, Meisha (committee member), Behrens, Sven (committee member), Deng, Yulin (committee member).
Subjects/Keywords: Particle; Bio-adhesion
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APA (6th Edition):
Shin, D. (2017). Effects of pollen and metal oxide pollen replica morphology on tailorable dry and wet adhesion. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/59776
Chicago Manual of Style (16th Edition):
Shin, Donglee. “Effects of pollen and metal oxide pollen replica morphology on tailorable dry and wet adhesion.” 2017. Doctoral Dissertation, Georgia Tech. Accessed March 01, 2021.
http://hdl.handle.net/1853/59776.
MLA Handbook (7th Edition):
Shin, Donglee. “Effects of pollen and metal oxide pollen replica morphology on tailorable dry and wet adhesion.” 2017. Web. 01 Mar 2021.
Vancouver:
Shin D. Effects of pollen and metal oxide pollen replica morphology on tailorable dry and wet adhesion. [Internet] [Doctoral dissertation]. Georgia Tech; 2017. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/1853/59776.
Council of Science Editors:
Shin D. Effects of pollen and metal oxide pollen replica morphology on tailorable dry and wet adhesion. [Doctoral Dissertation]. Georgia Tech; 2017. Available from: http://hdl.handle.net/1853/59776
University of New South Wales
18.
Deans, Rebecca.
Cause and effect of Asherman syndrome.
Degree: Women's & Children's Health, 2017, University of New South Wales
URL: http://handle.unsw.edu.au/1959.4/58839
;
https://unsworks.unsw.edu.au/fapi/datastream/unsworks:47714/SOURCE02?view=true
► Aim: To investigate the cause and effect of intrauterine adhesions (IUA) on reproductive function in women with Asherman syndrome (AS). Methods: Three studies have been…
(more)
▼ Aim: To investigate the cause and effect of intrauterine adhesions (IUA) on reproductive function in women with Asherman syndrome (AS). Methods: Three studies have been performed in women who have had fluoroscopically guided hysteroscopic synaechiolysis for AS in a tertiary referral unit: i) a retrospective cohort study investigating menstrual, fertility, obstetric and neonatal outcomes following surgery; ii) a retrospective cohort study investigating the efficacy of SeprafilmTM (Genzyme Corporation, Cambridge, MA) in improving menstrual fertility and obstetric outcomes;iii) an analysis of perfusion magnetic resonance imaging (MRI) investigating the total perfusion, ratio of perfusion, time to peak perfusion and gradient of perfusion to assess vascular markers of disease.Patients: 177 women diagnosed with AS, of whom 146 were trying to conceive. Results: The combined pregnancy rate was 106/146 (72.6%) women, and per unique woman, the live birth rate was 83/146 (56.8%), the miscarriage rate was 30/146 (20.5%) and the perinatal mortality rate was 4/146 (2.7%). there were 166 pregnancies in total resulting in; 97 live births, 48 miscarriages, 3 perinatal mortalities, 3 terminations of pregnancy for congenital anomalies, 4 ectopic pregnancies, and 10 ongoing pregnancies. In total, there were 98 babies born from 97 pregnancies in 83 women (1 set of twins). Maternal and perinatal morbidity included adherent placentation in 21/146 (14%), postpartum haemorrhage in 7/146 (9%), and hysterectomy in 4/146 (3%). Neonatal morbidity and mortality included prematurity in 25/98 (26%), and perinatal mortality in 4/98 (4%) of babies.
Adhesion barriers did not improve reproductive or obstetric outcomes. The uterine perfusion rates in women with grade 4 and above AS and inoperable IUA was significantly lower than those that had grade 1-3 AS and operable disease.Conclusion: Women with AS have improvement of their symptoms following surgery, including high rates of pregnancy. However, these pregnancies are complicated by maternal and fetal morbidity and mortality. Vascularity of the uterus may hold the key to understanding the pathological process behind this disorder. Perfusion MRI may also provide a prognostic tool in management of women with AS.
Advisors/Committee Members: Abbott, Jason, Women's & Children's Health, Faculty of Medicine, UNSW, Ledger, William, Women's & Children's Health, Faculty of Medicine, UNSW, Vancaillie, Thierry, Women's & Children's Health, Faculty of Medicine, UNSW.
Subjects/Keywords: Intrauterine; Asherman; Adhesion
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APA (6th Edition):
Deans, R. (2017). Cause and effect of Asherman syndrome. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/58839 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:47714/SOURCE02?view=true
Chicago Manual of Style (16th Edition):
Deans, Rebecca. “Cause and effect of Asherman syndrome.” 2017. Doctoral Dissertation, University of New South Wales. Accessed March 01, 2021.
http://handle.unsw.edu.au/1959.4/58839 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:47714/SOURCE02?view=true.
MLA Handbook (7th Edition):
Deans, Rebecca. “Cause and effect of Asherman syndrome.” 2017. Web. 01 Mar 2021.
Vancouver:
Deans R. Cause and effect of Asherman syndrome. [Internet] [Doctoral dissertation]. University of New South Wales; 2017. [cited 2021 Mar 01].
Available from: http://handle.unsw.edu.au/1959.4/58839 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:47714/SOURCE02?view=true.
Council of Science Editors:
Deans R. Cause and effect of Asherman syndrome. [Doctoral Dissertation]. University of New South Wales; 2017. Available from: http://handle.unsw.edu.au/1959.4/58839 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:47714/SOURCE02?view=true
Georgia Tech
19.
Taylor, Christine L.
ADHESION EVALUATION AND ASSEMBLY PROCESS DEVELOPMENT FOR PRINTED SILVER INK ON FLEXIBLE SUBSTRATES.
Degree: PhD, Mechanical Engineering, 2019, Georgia Tech
URL: http://hdl.handle.net/1853/64063
► Flexible substrates with printed electronics are being increasingly sought for the widespread and cost-effective use of flexible electronics. With printed ink on flexible substrates, several…
(more)
▼ Flexible substrates with printed electronics are being increasingly sought for the widespread and cost-effective use of flexible electronics. With printed ink on flexible substrates, several items need to be examined: synthesis of ink, deposition of ink, curing of ink, line and spacing of ink,
adhesion of ink, fracture strength of ink, electrical characteristics of ink, etc. Among these items,
adhesion of ink to the substrate plays an important role in the overall reliability of printed ink on flexible substrate.
In this work, the
adhesion and interfacial characteristics of printed conductors were determined though modified shear and peel experimental techniques. Modification to the tests were needed for handling the ink-jet printed films due to manufacturing considerations. (1) These films often are designed to be porous so that the films are more flexible by lower the stiffness. (2) Ink-jetting films often are composed of thin layers that are stacked-up to reach the desired thickness. (3) Depending on the tool and the file conversion to bitmap (or designated file extension) for the printer, the film may show indication of the path taken by the tool head with slight spaces between passes. A numerical model based on sequential crack growth was developed to examine how much the plastic deformation accounts for the experimentally measured peel energy. Lastly, a fully-additive printing process was demonstrated with resistors which resulted in around 6.5 MPa shear strength. For the assemblies, the joint strength of the ink for the joint to traces was stronger than the
adhesion strength of the ink to flexible substrate.
Advisors/Committee Members: Sitaraman, Suresh K. (advisor), Harris, Tequilia (committee member), Wang, Yan (committee member), Zhang, Chuck (committee member), Markondeya Raj, Pulugurtha (committee member).
Subjects/Keywords: Adhesion; Printed electronics
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APA (6th Edition):
Taylor, C. L. (2019). ADHESION EVALUATION AND ASSEMBLY PROCESS DEVELOPMENT FOR PRINTED SILVER INK ON FLEXIBLE SUBSTRATES. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/64063
Chicago Manual of Style (16th Edition):
Taylor, Christine L. “ADHESION EVALUATION AND ASSEMBLY PROCESS DEVELOPMENT FOR PRINTED SILVER INK ON FLEXIBLE SUBSTRATES.” 2019. Doctoral Dissertation, Georgia Tech. Accessed March 01, 2021.
http://hdl.handle.net/1853/64063.
MLA Handbook (7th Edition):
Taylor, Christine L. “ADHESION EVALUATION AND ASSEMBLY PROCESS DEVELOPMENT FOR PRINTED SILVER INK ON FLEXIBLE SUBSTRATES.” 2019. Web. 01 Mar 2021.
Vancouver:
Taylor CL. ADHESION EVALUATION AND ASSEMBLY PROCESS DEVELOPMENT FOR PRINTED SILVER INK ON FLEXIBLE SUBSTRATES. [Internet] [Doctoral dissertation]. Georgia Tech; 2019. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/1853/64063.
Council of Science Editors:
Taylor CL. ADHESION EVALUATION AND ASSEMBLY PROCESS DEVELOPMENT FOR PRINTED SILVER INK ON FLEXIBLE SUBSTRATES. [Doctoral Dissertation]. Georgia Tech; 2019. Available from: http://hdl.handle.net/1853/64063
University of Manchester
20.
James, Jenny J.
A proteomic investigation into the mechanisms of
VEGFâ€adhesion crosstalk in endothelial cells.
Degree: 2018, University of Manchester
URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:314298
► Adhesion of cells to the surrounding extra cellular matrix (ECM) is primarily mediated by integrin cell surface receptors, which mechanically link the ECM and actin…
(more)
▼ Adhesion of cells to the surrounding extra cellular
matrix (ECM) is primarily mediated by integrin cell surface
receptors, which mechanically link the ECM and actin cytoskeleton,
enabling the positioning of cells within tissues. Upon
integrinâ€ECM binding, signalling and adaptor proteins are
recruited, leading to the formation of intracellular integrin
adhesion complexes (IACs). These complexes and resulting integrin
signalling pathways coordinate their activity with growth factor
receptors and associated signalling pathways, regulating downstream
processes such as
adhesion, migration and proliferation. One
example of crosstalk between growth factor and integrin mediated
adhesion involves vascular endothelial growth factor (VEGF) in
endothelial cells (ECs), and contributes to angiogenesis, the
formation of new blood vessels from preâ€existing vasculature. A
better understanding of the factors and mechanisms involved in
VEGFâ€
adhesion crosstalk would enhance understanding of vascular
development, and highlight ways that therapeutics can be used to
treat angiogenic associated disorders. The aim of this project was
to identify mediators of VEGFâ€
adhesion crosstalk in ECs.
Initially, a protocol for human umbilical vein endothelial cell
(HUVEC) IAC enrichment was optimised. Briefly, HUVECs were plated
on fibronectin for two hours to allow IAC formation, followed by
crosslinking to stabilise IACs, cell body removal, and collection
of remaining IACs. Mass spectrometric analyses of enriched IACs
defined, for the first time, the HUVEC IAC composition, a network
of 297 proteins. This dataset was comparable to previously reported
IAC composition datasets, confirming successful identification of
IAC components. HUVEC IACs were then enriched following VEGF
treatment, and proteomic analysis revealed that the abundance of
only 1% of proteins changed ≥twoâ€fold. A complementary
phosphoproteomic strategy was adopted to analyse changes in protein
phosphorylation within
adhesion complexes following VEGF treatment.
Phosphoproteomic analysis of VEGFâ€induced enriched IAC and total
cell lysate samples revealed that over 18% of HUVEC IAC
phosphopeptides identified changed greater that ≥twoâ€fold.
Together, these data suggested that, while
adhesion complex
composition remains largely unchanged during VEGFadhesion
crosstalk, changes and signalling events occur mainly through
phosphorylation. From these proteomic and phosphoproteomic
datasets, a range of proteinâ€protein interaction networks were
constructed, statistical analyses employed, and detailed kinase
prediction analysis performed. A range of proteins were highlighted
as potentially important in VEGFâ€
adhesion crosstalk, including a
role for Src kinaseâ€mediated protein phosphorylation. To
investigate a role for Src kinase, VEGFinduced migration assays
were performed; Src and FAK inhibition reduced VEGFâ€induced
migration of FNâ€plated HUVECs, with combined kinase inhibition
showing greater effects. This study presents one of the first to
(1) perform a global proteomic analysis of…
Advisors/Committee Members: Canfield, Ann AE, Humphries, Martin, Canfield, Ann.
Subjects/Keywords: VEGF; angiogenesis; adhesion
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APA ·
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Manager
APA (6th Edition):
James, J. J. (2018). A proteomic investigation into the mechanisms of
VEGFâ€adhesion crosstalk in endothelial cells. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:314298
Chicago Manual of Style (16th Edition):
James, Jenny J. “A proteomic investigation into the mechanisms of
VEGFâ€adhesion crosstalk in endothelial cells.” 2018. Doctoral Dissertation, University of Manchester. Accessed March 01, 2021.
http://www.manchester.ac.uk/escholar/uk-ac-man-scw:314298.
MLA Handbook (7th Edition):
James, Jenny J. “A proteomic investigation into the mechanisms of
VEGFâ€adhesion crosstalk in endothelial cells.” 2018. Web. 01 Mar 2021.
Vancouver:
James JJ. A proteomic investigation into the mechanisms of
VEGFâ€adhesion crosstalk in endothelial cells. [Internet] [Doctoral dissertation]. University of Manchester; 2018. [cited 2021 Mar 01].
Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:314298.
Council of Science Editors:
James JJ. A proteomic investigation into the mechanisms of
VEGFâ€adhesion crosstalk in endothelial cells. [Doctoral Dissertation]. University of Manchester; 2018. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:314298
Rutgers University
21.
Vedula, Pavan, 1988-.
Spatially regulated β-actin translation in epithelia: analysis of adherens junction assembly, membrane cytoskeletal dynamics and tissue function.
Degree: PhD, Biology, 2016, Rutgers University
URL: https://rucore.libraries.rutgers.edu/rutgers-lib/50570/
► The β-actin mRNA contains a 28 nucleotide sequence in the 3′UTR termed the zipcode which, along with a trans acting factor called the Zipcode Binding…
(more)
▼ The β-actin mRNA contains a 28 nucleotide sequence in the 3′UTR termed the zipcode which, along with a trans acting factor called the Zipcode Binding Protein-1 (ZBP-1/IMP-1), regulates the spatio-temporal expression pattern of the β-actin. This mode of post-transcriptional gene regulation is required for several cellular processes including cell migration, cell-cell
adhesion, and cell-matrix
adhesion. Adding to the list of signaling cascades such as RhoA and Src, work in this thesis demonstrates E-cadherin endocytosis, and plausibly gene expression pathways regulating cell proliferation, converge on spatially regulated β-actin translation. E-cadherin endocytosis and recycling has been shown to be important during the maintenance of epithelial adherens junctions. However in this thesis, endocytosis of E-cadherin is shown to negatively regulate adherens junction assembly, while spatially regulated β-actin translation balances this by promoting actin filament anchoring of cadherin complexes. Mislocalization of β-actin translation impairs adherens junction maturation in epithelial cells following de novo cell-cell contact. These results have implications in development and disease where the assembly and maintenance of epithelial adherens junctions is essential. In order to quantify the extent of adherens junction assembly defects in epithelial cells following de novo contact, a Fluorescence Covariance Index based on calculating Pearson’s Correlation Coefficients was developed and validated using several components of the adherens junction complex. In addition to quantitating the morphological effects of mislocalizing β-actin translation on adherens junctions, the FCI analysis showed correlation of these morphological defects with loss of epithelial barrier function. Lastly, inhibiting dynamin mediated endocytosis rescued adherens junction structure and epithelial barrier function in cells with partially mislocalized β-actin. Using Matrigel embedded 3D cyst cultures of MDCK cells, spatially regulated β-actin translation was shown to have an effect on cell shape, size and proliferation during epithelial morphogenesis. These defects in assembling a normal 3D epithelium along with disorganized acto-myosin and tight junctions, point to a role for compartmentalized β-actin translation in regulating a mechanosensitive module – plausibly Hippo and/or YAP/TAZ pathways. Finally, collective cell migration, an important paradigm in several biological processes such as wound repair and development, was used to determine the role of spatially regulated β-actin translation in epithelial wound repair. Spatially regulated β-actin translation is shown to regulate actin flux at cell-cell adhesions in leader-follower cell pairs to control tissue migration rates. Although cells with partially mislocalized β-actin translation have a more active leading edge, this increased protrusive activity fails to translate into increased migratory rate. These data taken together suggest that collective cell migration in epithelial sheets is an emergent…
Advisors/Committee Members: Weber, Gregory F (chair), Rodriguez, Alexis J (internal member), Gao, Nan (internal member), Bonder, Edward M (internal member), Hinchcliffe, Edward (outside member), Hinchcliffe, Edward H (outside member).
Subjects/Keywords: Cell adhesion molecules
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APA (6th Edition):
Vedula, Pavan, 1. (2016). Spatially regulated β-actin translation in epithelia: analysis of adherens junction assembly, membrane cytoskeletal dynamics and tissue function. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/50570/
Chicago Manual of Style (16th Edition):
Vedula, Pavan, 1988-. “Spatially regulated β-actin translation in epithelia: analysis of adherens junction assembly, membrane cytoskeletal dynamics and tissue function.” 2016. Doctoral Dissertation, Rutgers University. Accessed March 01, 2021.
https://rucore.libraries.rutgers.edu/rutgers-lib/50570/.
MLA Handbook (7th Edition):
Vedula, Pavan, 1988-. “Spatially regulated β-actin translation in epithelia: analysis of adherens junction assembly, membrane cytoskeletal dynamics and tissue function.” 2016. Web. 01 Mar 2021.
Vancouver:
Vedula, Pavan 1. Spatially regulated β-actin translation in epithelia: analysis of adherens junction assembly, membrane cytoskeletal dynamics and tissue function. [Internet] [Doctoral dissertation]. Rutgers University; 2016. [cited 2021 Mar 01].
Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/50570/.
Council of Science Editors:
Vedula, Pavan 1. Spatially regulated β-actin translation in epithelia: analysis of adherens junction assembly, membrane cytoskeletal dynamics and tissue function. [Doctoral Dissertation]. Rutgers University; 2016. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/50570/
Michigan Technological University
22.
Narkar, Ameya R.
REVERSIBLY SWITCHING ADHESION OF SMART ADHESIVES INSPIRED BY MUSSEL ADHESIVE CHEMISTRY.
Degree: PhD, Department of Biomedical Engineering, 2018, Michigan Technological University
URL: https://digitalcommons.mtu.edu/etdr/741
► Catecholic groups in mussel adhesive proteins transition from being strongly adhesive in a reduced state under acidic conditions to being weakly adhesive in an…
(more)
▼ Catecholic groups in mussel adhesive proteins transition from being strongly adhesive in a reduced state under acidic conditions to being weakly adhesive in an oxidized state under basic conditions. Here, we exploit this pH responsive behavior of catechol and demonstrate that its oxidation state can be manipulated by incorporation of boronic acid to facilitate reversible transitions between strong and weak
adhesion. Our first approach involved the addition of 3- acrylamido phenylboronic acid (APBA) to dopamine methacrylamide (DMA) containing adhesives. The synthesized adhesives showed strong
adhesion to quartz surface in an acidic medium (pH 3), while weak
adhesion was observed on raising the pH to a basic value (pH 9), due to unavailability of catechol and boronic acid because of the formation of a reversible catechol-boronate complex. Boronic acid not only contributed to
adhesion at an acidic pH, but also allowed the catechol to reversibly interact with the surface in response to changing pH. In our second study, we demonstrated that addition of an anionic monomer, acrylic acid (AAc), preserved the reduced and adhesive state of catechol even at a neutral to mildly basic pH, while the addition of a cationic monomer, N-(aminopropyl) methacrylamide hydrochloride, led to the oxidized and weak adhesive state at higher basic pH values. This was due to the buffering of local pH offered by the incorporation of the ionic species, which affected the oxidation state of catechol. Although the ideal pH for formation of the complex is 9, it readily forms at neutral to mildly basic pH, leading to decreased
adhesion and limiting the adhesive’s application in physiological and marine pH environments. In our third 2 approach, adding elevated amounts of AAc to smart adhesives consisting of DMA and APBA led to strong
adhesion to quartz substrate at neutral to mildly basic pH. Moreover, the complex formed at pH 9 remained reversible and the interfacial binding could be tuned by changing the pH during successive contact cycles. pH 3 was required to break the complex and recover the strong adhesive property. Bulk adhesives analyzed in our first three approaches needed extended periods of incubation (up to 30 min) to switch between their adhesive and non-adhesive states. This is because infiltration of the pH media into the bulk polymer is limited by the slow process of diffusion. Finally, we fabricated a hybrid adhesive which was composed of gecko-inspired microstructured PDMS pillars (aspect ratios of 0.4-2) coated with the smart adhesive that we developed in our first approach. By tuning the aspect ratio of the bare templates, hybrid structures that showed strong, elevated
adhesion at pH 3, were obtained. The increased
adhesion was attributed to contact-splitting effects due to the micropatterning combined with the interfacial binding of the smart adhesive. On the other hand, formation of the complex, and the associated swelling of the adhesive together contributed to a significant decrease in
adhesion at pH 9. Additionally, the…
Advisors/Committee Members: Bruce P. Lee.
Subjects/Keywords: Mussel Adhesion; Catechol; Reversible adhesion; Smart Adhesive; Rapid adhesion; Biomaterials
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APA (6th Edition):
Narkar, A. R. (2018). REVERSIBLY SWITCHING ADHESION OF SMART ADHESIVES INSPIRED BY MUSSEL ADHESIVE CHEMISTRY. (Doctoral Dissertation). Michigan Technological University. Retrieved from https://digitalcommons.mtu.edu/etdr/741
Chicago Manual of Style (16th Edition):
Narkar, Ameya R. “REVERSIBLY SWITCHING ADHESION OF SMART ADHESIVES INSPIRED BY MUSSEL ADHESIVE CHEMISTRY.” 2018. Doctoral Dissertation, Michigan Technological University. Accessed March 01, 2021.
https://digitalcommons.mtu.edu/etdr/741.
MLA Handbook (7th Edition):
Narkar, Ameya R. “REVERSIBLY SWITCHING ADHESION OF SMART ADHESIVES INSPIRED BY MUSSEL ADHESIVE CHEMISTRY.” 2018. Web. 01 Mar 2021.
Vancouver:
Narkar AR. REVERSIBLY SWITCHING ADHESION OF SMART ADHESIVES INSPIRED BY MUSSEL ADHESIVE CHEMISTRY. [Internet] [Doctoral dissertation]. Michigan Technological University; 2018. [cited 2021 Mar 01].
Available from: https://digitalcommons.mtu.edu/etdr/741.
Council of Science Editors:
Narkar AR. REVERSIBLY SWITCHING ADHESION OF SMART ADHESIVES INSPIRED BY MUSSEL ADHESIVE CHEMISTRY. [Doctoral Dissertation]. Michigan Technological University; 2018. Available from: https://digitalcommons.mtu.edu/etdr/741
23.
Kyumurkov, Alexander.
Role of ICAP-1 in integrins' dynamic regulation, mechanosensing and contractility of osteoblast cells : Rôle d'ICAP-1 dans la régulation de la mécanosensibilité et de la contractilité des cellules ostéoblastiques via la dynamique des intégrins.
Degree: Docteur es, Biologie cellulaire, 2017, Université Grenoble Alpes (ComUE)
URL: http://www.theses.fr/2017GREAV057
► L'ICAP-1 est impliqué dans la dynamique de l'intégrine et la génération de force en contrôlant l'endocytose de l'intégrine grâce à la scission dépendant de nm23…
(more)
▼ L'ICAP-1 est impliqué dans la dynamique de l'intégrine et la génération de force en contrôlant l'endocytose de l'intégrine grâce à la scission dépendant de nm23 des puits endocycliques recouverts de chlatrine.L'ICAP-1 a été identifié comme un partenaire spécifique de l'intégrine b1 (Degani et al., 2002, Zhang et Hemler, 1999). Nous avons déjà montré que l'ICAP-1 est impliqué dans la réponse mécanisée aux cellules et la différenciation cellulaire d'une manière dépendante de l'intégrine b1 (Bouvard et al., 2007; Brunner et al., 2011; Faurobert et al., 2013; Millon-Frémillon et al. , 2008; Renz et al., 2015). Cependant, comme ICAP-1 est également capable d'adapter la migration cellulaire en réponse à la rigidité du substrat d'une manière indépendante de la β1-intégrine (Bouin et al., 2017), nous avons spéculé sur un rôle plus général de l'ICAP-1 dans l'adhésion cellulaire et dynamique d'adhérence focale. Pour cela, nous avons créé un environnement cellulaire où l'intégrine b1 et / ou l'ICAP-1 étaient absentes en utilisant quatre lignées cellulaires: les ostéoblastes WT, les cellules ostéoblastes KO de l'intégrine b1, les cellules ostéoblastes KAP ICAP-1 et les cellules ostéoblastes double KO b1 / ICAP-1 afin de surveiller le comportement de l'intégrine b3. Comme prévu, l'épuisement de l'intégrine b1 est associé à la perte d'étalement cellulaire et à la génération de force selon la mesure de la microscopie par force de traction. De manière surprenante, la suppression supplémentaire de ICAP-1 (b1 intégrine et ICAP-1 KO) conduit à la restauration de l'étalement cellulaire et de la génération de force qui dépend de l'intégrine b3. Ces forces médiées par l'intégrine b3 sont corrélées avec la diffusion lente de l'intégrine b3 dans les sites d'adhésion et le renouvellement lent de l'adhésion focale contenant l'intégrine b3 (FRAP / TIRF / vidéomicroscopie). Nous avons abordé la question de savoir si ICAP-1 pourrait réguler l'endocytose de l'intégrine b3 puisque ICAP-1 interagit avec nm23-H2 (Fournier et al., 2002), un nucléoside diphosphate kinases (NDPK) impliqué dans l'endocytose à médiation par dynamine en produisant du GTP à travers l'adénosine triphosphate (ATP) de conversion du diphosphate de guanosine (PIB) (Boissan et al., 2014). Nous montrons que la suppression de nm23 ou de dynamine ou de chlatrine dans les cellules épuisées dans l'intégrine b1 est capable d'imiter la perte combinée de l'intégrine b1 et de l'ICAP-1 en rétablissant l'étalement cellulaire, la génération de force et la dynamique de l'intégrine b3. Pour confirmer l'implication de l'ICAP-1 dans l'endocytose de l'intégrine b3, nous montrons que l'absorption de l'anticorps de l'intégrine b3 est efficacement bloquée dans les cellules épuisées dans ICAP-1. Nos résultats suggèrent que ICAP-1 pourrait être impliqué dans la dynamique de l'intégrine et la génération de force en contrôlant l'endocytose de l'intégrine grâce à la scission dépendant de nm23 des puits endocytaires de la couche de chlatrine.
ICAP-1 is involved in integrin dynamics and force…
Advisors/Committee Members: Albiges-Rizo, Corinne (thesis director), Planus, Emmanuelle (thesis director).
Subjects/Keywords: Adhesion; Contractilité; Integrines; Endocytose; Dynamique d'adhesion; Adhesion; Contractility; Integrins; Endocytosis; Adhesion dynamics; 570
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APA (6th Edition):
Kyumurkov, A. (2017). Role of ICAP-1 in integrins' dynamic regulation, mechanosensing and contractility of osteoblast cells : Rôle d'ICAP-1 dans la régulation de la mécanosensibilité et de la contractilité des cellules ostéoblastiques via la dynamique des intégrins. (Doctoral Dissertation). Université Grenoble Alpes (ComUE). Retrieved from http://www.theses.fr/2017GREAV057
Chicago Manual of Style (16th Edition):
Kyumurkov, Alexander. “Role of ICAP-1 in integrins' dynamic regulation, mechanosensing and contractility of osteoblast cells : Rôle d'ICAP-1 dans la régulation de la mécanosensibilité et de la contractilité des cellules ostéoblastiques via la dynamique des intégrins.” 2017. Doctoral Dissertation, Université Grenoble Alpes (ComUE). Accessed March 01, 2021.
http://www.theses.fr/2017GREAV057.
MLA Handbook (7th Edition):
Kyumurkov, Alexander. “Role of ICAP-1 in integrins' dynamic regulation, mechanosensing and contractility of osteoblast cells : Rôle d'ICAP-1 dans la régulation de la mécanosensibilité et de la contractilité des cellules ostéoblastiques via la dynamique des intégrins.” 2017. Web. 01 Mar 2021.
Vancouver:
Kyumurkov A. Role of ICAP-1 in integrins' dynamic regulation, mechanosensing and contractility of osteoblast cells : Rôle d'ICAP-1 dans la régulation de la mécanosensibilité et de la contractilité des cellules ostéoblastiques via la dynamique des intégrins. [Internet] [Doctoral dissertation]. Université Grenoble Alpes (ComUE); 2017. [cited 2021 Mar 01].
Available from: http://www.theses.fr/2017GREAV057.
Council of Science Editors:
Kyumurkov A. Role of ICAP-1 in integrins' dynamic regulation, mechanosensing and contractility of osteoblast cells : Rôle d'ICAP-1 dans la régulation de la mécanosensibilité et de la contractilité des cellules ostéoblastiques via la dynamique des intégrins. [Doctoral Dissertation]. Université Grenoble Alpes (ComUE); 2017. Available from: http://www.theses.fr/2017GREAV057
NSYSU
24.
Peng, Shi-wei.
Investigation of Bio-Inspired Polydopamine Using Atomic Force Microscopy.
Degree: Master, Chemistry, 2015, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0621115-154800
► Dopamine solution will conduct autoxidation and self-polymerization to form polydopamine particles in alkaline tris-(hydroxymethyl)aminomethane (Tris) buffer solution. At the same time, the ploydopamine deposition onto…
(more)
▼ Dopamine solution will conduct autoxidation and self-polymerization to form polydopamine particles in alkaline tris-(hydroxymethyl)aminomethane (Tris) buffer solution. At the same time, the ploydopamine deposition onto various substrates via
covalent bond or non-covalent bond such as hydrogen bond, electrostatic interaction, Ï-Ï interaction, van der waal interaction. In this study, we used atomically flat mica to investigate the growth mode and the growth mechanism of polydopamine by atomic force microscopy (AFM), x-ray photoelectron spectroscopy (XPS), and contact angle measurment. On the other hand, we also used polydopamine-modified AFM tip to
detect the interaction with various substrates and designed surface patterning on polydimethylsiloxane (PDMS). The results show that the monolayer of polydopamine film is completed within 300 sec and the roughness had significantly changed compared with bare mica. The polydopamine-modified AFM tip had the strongest interaction with
Highly Oriented Pyrolytic Graphite (HOPG) due to the strong Ï-Ï interaction between the aromatic rings structure on polydopamine and the planar structure of sp2 orbital on HOPG. Finally, the surface patterning on PDMS by polydopamine successfully modified Au nanoparticles and Ag nanoparticles on specific area.
Advisors/Committee Members: Shang-Wu Ding (chair), Shuchen Hsieh (committee member), Huey-Shan Hung (chair).
Subjects/Keywords: patterning; AFM; polydopamine; polydimethylsiloxane; adhesion
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APA (6th Edition):
Peng, S. (2015). Investigation of Bio-Inspired Polydopamine Using Atomic Force Microscopy. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0621115-154800
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Peng, Shi-wei. “Investigation of Bio-Inspired Polydopamine Using Atomic Force Microscopy.” 2015. Thesis, NSYSU. Accessed March 01, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0621115-154800.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Peng, Shi-wei. “Investigation of Bio-Inspired Polydopamine Using Atomic Force Microscopy.” 2015. Web. 01 Mar 2021.
Vancouver:
Peng S. Investigation of Bio-Inspired Polydopamine Using Atomic Force Microscopy. [Internet] [Thesis]. NSYSU; 2015. [cited 2021 Mar 01].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0621115-154800.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Peng S. Investigation of Bio-Inspired Polydopamine Using Atomic Force Microscopy. [Thesis]. NSYSU; 2015. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0621115-154800
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
25.
Kawamoto, Eiji.
LFA-1 and Mac-1 integrins bind to the serine/threonine-rich domain of thrombomodulin.
Degree: 博士(医学), 2017, Mie University / 三重大学
URL: http://hdl.handle.net/10076/00017114
► LFA-1 (aLb2) and Mac-1 (aMb2) integrins regulate leukocyte trafficking in health and disease by binding primarily to IgSF ligand ICAM-1 and ICAM-2 on endothelial cells.…
(more)
▼ LFA-1 (aLb2) and Mac-1 (aMb2) integrins regulate leukocyte trafficking in health and disease by binding primarily to IgSF ligand ICAM-1 and ICAM-2 on endothelial cells. Here we have shown that the anticoagulant molecule thrombomodulin (TM), found on the surface of endothelial cells, functions as a potentially new ligand for leukocyte integrins. We generated a recombinant extracellular domain of human TM and Fc fusion protein (TM-domains 123-Fc), and showed that pheripheral blood mononuclear cells (PBMCs) bind to TM-domains 123-Fc dependent upon integrin activation. We then demonstrated that aL integrin-blocking mAb, aM integrin-blocking mAb, and b2 integrin-blocking mAb inhibited the binding of PBMCs to TM-domains 123-Fc. Furthermore, we show that the serine/threonine-rich domain (domain 3) of TM is required for the interaction with the LFA-1 (aLb2) and Mac-1 (aMb2) integrins to occur on PBMCs. These results demonstrate that the LFA-1 and Mac-1 integrins on leukocytes bind to TM, thereby establishing the molecular and structural basis underlying LFA-1 and Mac-1 integrin interaction with TM on endothelial cells. In fact, integrin-TM interactions might be involved in the dynamic regulation of leukocyte adhesion with endothelial cells.
本文 / Department of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine; Emergency and Critical Care Center, Mie University Hospital
8p
Subjects/Keywords: Integrin; Thrombomodulin; Inflammation; Adhesion molecules
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APA (6th Edition):
Kawamoto, E. (2017). LFA-1 and Mac-1 integrins bind to the serine/threonine-rich domain of thrombomodulin. (Thesis). Mie University / 三重大学. Retrieved from http://hdl.handle.net/10076/00017114
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Kawamoto, Eiji. “LFA-1 and Mac-1 integrins bind to the serine/threonine-rich domain of thrombomodulin.” 2017. Thesis, Mie University / 三重大学. Accessed March 01, 2021.
http://hdl.handle.net/10076/00017114.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Kawamoto, Eiji. “LFA-1 and Mac-1 integrins bind to the serine/threonine-rich domain of thrombomodulin.” 2017. Web. 01 Mar 2021.
Vancouver:
Kawamoto E. LFA-1 and Mac-1 integrins bind to the serine/threonine-rich domain of thrombomodulin. [Internet] [Thesis]. Mie University / 三重大学; 2017. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/10076/00017114.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Kawamoto E. LFA-1 and Mac-1 integrins bind to the serine/threonine-rich domain of thrombomodulin. [Thesis]. Mie University / 三重大学; 2017. Available from: http://hdl.handle.net/10076/00017114
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Alberta
26.
EL Gowini, Mohamed MT.
Theoretical and Experimental Investigation on the Use of
Surface Acoustic Wave Sensors for Evaluating the Adhesion of SU-8
Thin Films.
Degree: PhD, Department of Mechanical Engineering, 2015, University of Alberta
URL: https://era.library.ualberta.ca/files/c7s75dc526
► This research investigates the use of a SU-8/AlN/Si SAW sensor for evaluating the adhesion of SU-8 thin films. A theoretical model is developed to plot…
(more)
▼ This research investigates the use of a SU-8/AlN/Si
SAW sensor for evaluating the adhesion of SU-8 thin films. A
theoretical model is developed to plot the wave dispersion profile
for the SU-8/AlN/Si configuration. A spring interface model is
utilized to represent the SU-8/AlN interface using a series of
massless springs with stiffness K (N/m3). The value of the
interface spring stiffness K is changed to represent different
levels of SU-8 adhesion. The wave dispersion profiles for the
intermediate adhesion levels are plotted using the theoretical
model. The change in wave velocity due to the change in adhesion of
the SU-8 thin film is evaluated. The sensitivities of different
configurations of the SU-8/AlN/Si SAW sensors are investigated.
Four SAW sensor designs are selected to evaluate the adhesion of
the SU-8 thin film. The four SAW sensors operate in the frequency
range of 84-208MHz. A process flow for fabricating the SAW sensors
at the University of Alberta micro-and nanofabrication facility
“nanofab” is developed. The fabricated sensors are packaged using
wire bonding to allow measurement of their frequency responses
using a Vector Network Analyzer. For each of the four SAW sensor
designs two sensor configurations will be developed. In one
configuration the SU-8 film will be patterned on top of a thin gold
film on the surface of the AlN/Si layers and in the second
configuration the gold film will be coated with an Omnicoat layer
prior to patterning the SU-8 film. Omnicoat is an adhesion promoter
that is widely used to improve the adhesion of SU-8 to gold. The
frequency responses from both sensor configurations are measured
for each of the four SAW sensor designs and the frequency shift is
evaluated. The frequency shift illustrates the change in adhesion
of the SU-8 film with and without omnicoat. The phase velocity
values also shift to a higher value for the sensor configurations
without omnicoat. As the adhesion of the SU-8 film drops in the
absence of omnicoat the stresses transferred to the SU-8 layer drop
and accordingly the wave propagation is concentrated in the AlN/Si
layers, which have a higher wave velocity than SU-8. However, in
the presence of omnicoat the adhesion of the SU-8 layer improves
and the surface acoustic wave propagates in the SU-8 layer, which
leads to a drop in the phase velocity. The theoretical model is
used to find the equivalent interface spring stiffness values for
the two cases of SU-8 adhesion i.e. with and without omnicoat. This
is accomplished by curve fitting the dispersion curves to the phase
velocity values for from both sensor configurations. The equivalent
interface spring stiffness values are found to be 8.0992 x109
N/m3 and 7.9492x109 N/m3 for the SAW sensors with omnicoat and
without omnicoat, respectively. These values indicate that when
omnicoat is used as an adhesion promoter for SU-8 the interface
spring stiffness increases due to the improved adhesion. However,
without omnicoat the adhesion of the SU-8 layer drops, which
corresponds to the lower interface spring…
Subjects/Keywords: Adhesion; Surface Acoustic Waves; MEMS
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
EL Gowini, M. M. (2015). Theoretical and Experimental Investigation on the Use of
Surface Acoustic Wave Sensors for Evaluating the Adhesion of SU-8
Thin Films. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/c7s75dc526
Chicago Manual of Style (16th Edition):
EL Gowini, Mohamed MT. “Theoretical and Experimental Investigation on the Use of
Surface Acoustic Wave Sensors for Evaluating the Adhesion of SU-8
Thin Films.” 2015. Doctoral Dissertation, University of Alberta. Accessed March 01, 2021.
https://era.library.ualberta.ca/files/c7s75dc526.
MLA Handbook (7th Edition):
EL Gowini, Mohamed MT. “Theoretical and Experimental Investigation on the Use of
Surface Acoustic Wave Sensors for Evaluating the Adhesion of SU-8
Thin Films.” 2015. Web. 01 Mar 2021.
Vancouver:
EL Gowini MM. Theoretical and Experimental Investigation on the Use of
Surface Acoustic Wave Sensors for Evaluating the Adhesion of SU-8
Thin Films. [Internet] [Doctoral dissertation]. University of Alberta; 2015. [cited 2021 Mar 01].
Available from: https://era.library.ualberta.ca/files/c7s75dc526.
Council of Science Editors:
EL Gowini MM. Theoretical and Experimental Investigation on the Use of
Surface Acoustic Wave Sensors for Evaluating the Adhesion of SU-8
Thin Films. [Doctoral Dissertation]. University of Alberta; 2015. Available from: https://era.library.ualberta.ca/files/c7s75dc526
University of Manchester
27.
Jethwa, Devina.
The Role and Regulation of the Focal Adhesion
Network.
Degree: 2018, University of Manchester
URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:314799
► This abstract is for the thesis entitled ‘The role and regulation of the focal adhesion network’ by Devina Jethwa. The thesis is submitted in 2017…
(more)
▼ This abstract is for the thesis entitled ‘The
role and regulation of the focal adhesion network’ by Devina
Jethwa. The thesis is submitted in 2017 for the degree of Doctor of
Philosophy in the Faculty of Biology, Medicine and Health, School
of Biological Sciences, University of Manchester. Cells sense and
interpret environmental signals to influence a number of cellular
processes including migration, proliferation and differentiation.
Focal adhesions (FAs) are large integrin based adhesion receptors
that link the extracellular matrix to the contractile actin
cytoskeleton. Here, FAs can sense mechanical changes of the
underlying matrix and translate this information into a cellular
response, a process known as mechanotransduction. However, the
molecular processes that co-ordinate mechanotransduction are yet to
be elucidated. Talin and vinculin are two multidomain proteins that
form the core of the FA and are thought to be involved in
mechanotransduction. The first results chapter focuses on the
force-dependent interactions between talin, vinculin and actin.
Talin’s R2R3 domains were identified as key mechanosensitive
vinculin binding sites that are exposed upon the application of
force. Upon vinculin binding to R2R3, talin’s central actin
binding site (ABS2) is exposed allowing actin to bind to talin and
link to the underlying substrate. The association of actin at
talin’s ABS2 is required for the transmission of actomyosin
tensin onto the underlying substrate as traction force. The second
results chapter focuses on the force-independent mechanisms of
talin and vinculin activation and recruitment. Through the
generation of a force free system, we revealed that actomyosin
forces are not essential for talin to expose potential vinculin
binding sites. Following talin activation, vinculin can bind to
talin’s R3 domain to trigger vinculin activation. Once activated,
actomyosin forces are required for talin and vinculin to recruit
the FA plaque and stabilse the FA. Finally, we assembled a core
subset of FA proteins into a mathematical rule based model (RBM).
Using a rule based modelling technique, we were able to create the
first large-scale adhesome with talin and vinculin playing central
roles. Protein turnover data was used to link interactions and
simulate the dynamic exchange of various talin and vinculin mutants
and how the FA network can affect cellular processes (i.e.,
cellular migration). Together, these findings increase our
knowledge in the processes that coordinate adhesion signalling and
in particular, mechanotransduction. By understanding these
processes, we could develop novel therapies to target diseases such
as fibrosis, cancer and atherosclerosis.
Appendix on CD disk
Advisors/Committee Members: SCHWARTZ, JEAN-MARC J, Ballestrem, Christoph, Schwartz, Jean-Marc.
Subjects/Keywords: Focal Adhesion; Talin; Vinculin
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APA ·
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Manager
APA (6th Edition):
Jethwa, D. (2018). The Role and Regulation of the Focal Adhesion
Network. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:314799
Chicago Manual of Style (16th Edition):
Jethwa, Devina. “The Role and Regulation of the Focal Adhesion
Network.” 2018. Doctoral Dissertation, University of Manchester. Accessed March 01, 2021.
http://www.manchester.ac.uk/escholar/uk-ac-man-scw:314799.
MLA Handbook (7th Edition):
Jethwa, Devina. “The Role and Regulation of the Focal Adhesion
Network.” 2018. Web. 01 Mar 2021.
Vancouver:
Jethwa D. The Role and Regulation of the Focal Adhesion
Network. [Internet] [Doctoral dissertation]. University of Manchester; 2018. [cited 2021 Mar 01].
Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:314799.
Council of Science Editors:
Jethwa D. The Role and Regulation of the Focal Adhesion
Network. [Doctoral Dissertation]. University of Manchester; 2018. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:314799
Universidade Estadual de Campinas
28.
Caiado, Ana Carolina Rocha Lima.
Avaliação da integridade marginal e dureza Knoop de cimentos resinosos usados na fixação de pinos de fibra de vidro anatomicamente reembasados: Marginal integrity and Knoop hardness of luting cements used in the fixation of anatomically relined fiber posts.
Degree: 2010, Universidade Estadual de Campinas
URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/288153
► Abstract: Preformed fiber-reinforced root canal posts have increased in popularity as an alternative to metal posts. This is partly due to a modulus of elasticity…
(more)
▼ Abstract: Preformed fiber-reinforced root canal posts have increased in popularity as an alternative to metal posts. This is partly due to a modulus of elasticity that is closer to that of dentin when compared to metal posts. The employment of materials with similar mechanical properties creates a homogenous biomechanical unit which favors uniform stress distribution and reduces the incidence of root fracture and micro-leakage. In spite of positive outcomes in terms of laboratory and clinical results documented in retrospective and prospective studies, major concerns around bonding procedures still remain. The dislodging of fiber posts from the root canal continues to be the main cause of failure of fiber post-retained restorations. A chair side clinical procedure that involves the confectioning of an anatomically shaped relined post was developed to compensate the mismatch between fiber post and post space, offering a more conservative preparation for the remaining root structure. This technique has been described to compensate light limitations because the composite resin used to anatomically shape the fiber-post is light-cured immediately after relining, outside of the root canal, before cementation. Among the possible advantages of light curing the relined fiber post outside of the root canal and reducing the cement layer thickness are the increase of monomer conversion, the reduction of polymerization shrinkage, and the presence of bubbles in the cement layer. Therefore, the aim of the present study was to: (1) Compare marginal integrity of conventional and anatomically relined fiberposts to root canal dentin using scanning electron microscopy; (2) Evaluate and compare Knoop's Hardness of two dual-cure cements with for conventional and anatomically relined fiberposts in the presence and absence of light; and (3) Compare the marginal integrity between a self adhesive cement and root canal dentin after cementation of anatomic fiber posts relined with either methacrylate or silorane based composite resin. It was possible to conclude that: (1) Marginal integrity of relined fiber posts to root dentin is superior compared to conventional fiber posts; (2) The technique, activation mode and cement type were factors that affected Knoop hardness for cervical and middle root thirds. The apical third was exclusively influenced by activation mode; and (3) The material used for anatomically relining fiber posts did not significantly affect marginal integrity to root canal walls
Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Goes, Mario Fernando de, 1954- (advisor), Universidade Estadual de Campinas. Faculdade de Odontologia de Piracicaba (institution), Programa de Pós-Graduação em Clínica Odontológica (nameofprogram), Carvalho, Ricardo Marins de (committee member), Imura, Noboru (committee member), Giannini, Marcelo (committee member), Almeida, José Flávio Affonso de (committee member).
Subjects/Keywords: Adesão; Adhesion
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APA ·
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APA (6th Edition):
Caiado, A. C. R. L. (2010). Avaliação da integridade marginal e dureza Knoop de cimentos resinosos usados na fixação de pinos de fibra de vidro anatomicamente reembasados: Marginal integrity and Knoop hardness of luting cements used in the fixation of anatomically relined fiber posts. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/288153
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Caiado, Ana Carolina Rocha Lima. “Avaliação da integridade marginal e dureza Knoop de cimentos resinosos usados na fixação de pinos de fibra de vidro anatomicamente reembasados: Marginal integrity and Knoop hardness of luting cements used in the fixation of anatomically relined fiber posts.” 2010. Thesis, Universidade Estadual de Campinas. Accessed March 01, 2021.
http://repositorio.unicamp.br/jspui/handle/REPOSIP/288153.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Caiado, Ana Carolina Rocha Lima. “Avaliação da integridade marginal e dureza Knoop de cimentos resinosos usados na fixação de pinos de fibra de vidro anatomicamente reembasados: Marginal integrity and Knoop hardness of luting cements used in the fixation of anatomically relined fiber posts.” 2010. Web. 01 Mar 2021.
Vancouver:
Caiado ACRL. Avaliação da integridade marginal e dureza Knoop de cimentos resinosos usados na fixação de pinos de fibra de vidro anatomicamente reembasados: Marginal integrity and Knoop hardness of luting cements used in the fixation of anatomically relined fiber posts. [Internet] [Thesis]. Universidade Estadual de Campinas; 2010. [cited 2021 Mar 01].
Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/288153.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Caiado ACRL. Avaliação da integridade marginal e dureza Knoop de cimentos resinosos usados na fixação de pinos de fibra de vidro anatomicamente reembasados: Marginal integrity and Knoop hardness of luting cements used in the fixation of anatomically relined fiber posts. [Thesis]. Universidade Estadual de Campinas; 2010. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/288153
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universidade Estadual de Campinas
29.
Feitosa, Victor Pinheiro, 1987-.
Evaluation of acidic functional monomers with spacer chains with different hydrophilicities and lengths = Avaliação de monômeros funcionais ácidos com cadeias espaçadoras de diferentes hidrofilias e comprimentos: Avaliação de monômeros funcionais ácidos com cadeias espaçadoras de diferentes hidrofilias e comprimentos.
Degree: 2013, Universidade Estadual de Campinas
URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/288445
► Abstract: The aim of this study was to evaluate the effects of spacer chain (length and polarity) of five phosphoric functional monomers, MEP (two carbons),…
(more)
▼ Abstract: The aim of this study was to evaluate the effects of spacer chain (length and polarity) of five phosphoric functional monomers, MEP (two carbons), MDP (ten carbons), MDDP (twelve carbons), MTEP (more hydrophilic spacer chain) and CAP-P (intermediate hydrophilicity spacer). It was assessed the chemical bonding with dentin and calcium, the physicochemical properties of one-step self-etch adhesives and the
adhesion to dentin. This investigation was separated in 3 chapters. Chapter 1 verified the influence of spacer chain of functional monomers on the degree of conversion, ultimate tensile strength (UTS), water sorption and wettability of experimental all-in-one adhesives. Light-activation in all chapters was undertaken using halogen-lamp with minimal irradiance of 600mW/cm². All data were submitted to one-way ANOVA and Tukey's test (p<0.05). The outcomes showed that different monomers promote similar degree of conversion when added in same molar percentage. Nevertheless, their UTS were distinct, with high values for monomers able to promote intermolecular interactions. The water sorption and dentin wettability were greater with more hydrophilic (polar) monomers. In chapter 2, the purpose was to assess the chemical interaction of studied functional monomers with calcium and dentin. It was analyzed the formation of monomer-calcium salts through evaluation of free-calcium (Ca2+) in atomic absorption spectroscopy (AAS), the interaction of aqueous monomer-containing solutions with dentin slabs before and after rinsing with water and ethanol by means of Fourier-transform infrared spectroscopy (FTIR) and the presence of monomers onto the dentin slabs was imaged in scanning electron microscopy. The results displayed better ionic bond with calcium with monomers based on long and less polar spacer chains. The stability of such linkages was affected only for the monomer with short spacer chain. The hydrophilicity of spacer chain did not affect the stability of the chemical bond. In chapter 3, the aim was to evaluate the formation of bonds between monomers and calcium as well as the bonding performance of all-in-one adhesives using the investigated monomers. It was analyzed the microtensile bond strength after 24h and one year of water storage, the AAS absorbance of free-calcium, the micropermeability and the nanoleakage of adhesives. The outcomes showed that initial bond strength and formation of linkages with calcium were lower for monomers with short and polar spacer chains. The bond strength was reduced after aging only for the monomer with highly hydrophilic spacer chain, likely due to higher micropermeability. There was little nanoleakage only for monomers with long and hydrophobic spacer chains. As conclusion, it was observed that more hydrophilic and short spacer chains jeopardize the chemical bonding, the
adhesion and the physicochemical properties of acidic functional monomers
Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Correr, Americo Bortolazzo, 1981- (advisor), Sinhoreti, Mário Alexandre Coelho, 1969- (coadvisor), Universidade Estadual de Campinas. Faculdade de Odontologia de Piracicaba (institution), Programa de Pós-Graduação em Materiais Dentários (nameofprogram), Ogliari, Fabrício Aulo (committee member), Santiago, Sérgio Lima (committee member), Giannini, Marcelo (committee member), Naves, Lucas Zago (committee member).
Subjects/Keywords: Adesão; Dentina; Adhesion; Dentin
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APA (6th Edition):
Feitosa, Victor Pinheiro, 1. (2013). Evaluation of acidic functional monomers with spacer chains with different hydrophilicities and lengths = Avaliação de monômeros funcionais ácidos com cadeias espaçadoras de diferentes hidrofilias e comprimentos: Avaliação de monômeros funcionais ácidos com cadeias espaçadoras de diferentes hidrofilias e comprimentos. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/288445
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Feitosa, Victor Pinheiro, 1987-. “Evaluation of acidic functional monomers with spacer chains with different hydrophilicities and lengths = Avaliação de monômeros funcionais ácidos com cadeias espaçadoras de diferentes hidrofilias e comprimentos: Avaliação de monômeros funcionais ácidos com cadeias espaçadoras de diferentes hidrofilias e comprimentos.” 2013. Thesis, Universidade Estadual de Campinas. Accessed March 01, 2021.
http://repositorio.unicamp.br/jspui/handle/REPOSIP/288445.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Feitosa, Victor Pinheiro, 1987-. “Evaluation of acidic functional monomers with spacer chains with different hydrophilicities and lengths = Avaliação de monômeros funcionais ácidos com cadeias espaçadoras de diferentes hidrofilias e comprimentos: Avaliação de monômeros funcionais ácidos com cadeias espaçadoras de diferentes hidrofilias e comprimentos.” 2013. Web. 01 Mar 2021.
Vancouver:
Feitosa, Victor Pinheiro 1. Evaluation of acidic functional monomers with spacer chains with different hydrophilicities and lengths = Avaliação de monômeros funcionais ácidos com cadeias espaçadoras de diferentes hidrofilias e comprimentos: Avaliação de monômeros funcionais ácidos com cadeias espaçadoras de diferentes hidrofilias e comprimentos. [Internet] [Thesis]. Universidade Estadual de Campinas; 2013. [cited 2021 Mar 01].
Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/288445.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Feitosa, Victor Pinheiro 1. Evaluation of acidic functional monomers with spacer chains with different hydrophilicities and lengths = Avaliação de monômeros funcionais ácidos com cadeias espaçadoras de diferentes hidrofilias e comprimentos: Avaliação de monômeros funcionais ácidos com cadeias espaçadoras de diferentes hidrofilias e comprimentos. [Thesis]. Universidade Estadual de Campinas; 2013. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/288445
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Penn State University
30.
Chorpenning, Katherine Ann.
Platelet Adhesion to Polyurethane Urea Under Cardiac Pulse.
Degree: 2010, Penn State University
URL: https://submit-etda.libraries.psu.edu/catalog/10765
► Platelet adhesion to an artificial surface is a key event in thrombus development for cardiovascular devices. Polyurethane urea (PUU) is one widely used biomaterial in…
(more)
▼ Platelet
adhesion to an artificial surface is a key event in thrombus development for cardiovascular devices. Polyurethane urea (PUU) is one widely used biomaterial in the medical device industry today due to its desirable mechanical and blood compatibility properties. The goal of the present work is to test the platelet
adhesion properties of PUU under simulated physiologic conditions. A rotating disk system (RDS) is used to deliver a “cardiac pulse” to a stainless steel disk with PUU attached to the bottom surface. This pulse simulates the mean velocities typically seen at the inlet of a left ventricular assist device during the cardiac cycle. Using a rotating disk provides a well-defined flow that spans a large shear rate range. The metal disk and material rotate in platelet rich plasma (PRP) obtained from bovine blood. Platelet adherence is quantified by immunofluorescent labeling using CAPP2A mouse anti-bovine αIIbβ3 antibody and Alexa-Fluor 488 donkey anti-mouse IgG. Two approaches were used to identify shear rates with high levels of platelet
adhesion. The first method applied a cardiac pulse continuously to a 15 mm disk for 2 hr. The average
adhesion coefficient (AC%) was 0.60% at the center, which is comparable to the values seen for a steady 2 hr rotation at 283 rpm (AC% = 0.36%). In the second study, platelets were pre-adhered to the material surface and a cardiac pulse was applied continuously for 30 min. At the disk center, 33% of the original platelet number remained following the 30 min pulse. This value decreased to 10% at 300 s-1 (3 mm radius) and 5% at 840 s-1 (8 mm radius). Steady rotation and cardiac pulse experiments all showed low platelet
adhesion for shear rates higher than 500 s-1. This is the highest shear rate at which a platelet can adhere to a polyurethane surface and is a current standard for many cardiovascular applications.
Advisors/Committee Members: Keefe B Manning, Thesis Advisor/Co-Advisor, Keefe B Manning, Thesis Advisor/Co-Advisor, Margaret June Slattery, Thesis Advisor/Co-Advisor, Steven Deutsch, Thesis Advisor/Co-Advisor.
Subjects/Keywords: platelet; adhesion
Record Details
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Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Chorpenning, K. A. (2010). Platelet Adhesion to Polyurethane Urea Under Cardiac Pulse. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/10765
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Chorpenning, Katherine Ann. “Platelet Adhesion to Polyurethane Urea Under Cardiac Pulse.” 2010. Thesis, Penn State University. Accessed March 01, 2021.
https://submit-etda.libraries.psu.edu/catalog/10765.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Chorpenning, Katherine Ann. “Platelet Adhesion to Polyurethane Urea Under Cardiac Pulse.” 2010. Web. 01 Mar 2021.
Vancouver:
Chorpenning KA. Platelet Adhesion to Polyurethane Urea Under Cardiac Pulse. [Internet] [Thesis]. Penn State University; 2010. [cited 2021 Mar 01].
Available from: https://submit-etda.libraries.psu.edu/catalog/10765.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Chorpenning KA. Platelet Adhesion to Polyurethane Urea Under Cardiac Pulse. [Thesis]. Penn State University; 2010. Available from: https://submit-etda.libraries.psu.edu/catalog/10765
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
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