subject:(Adducts)

University of Lethbridge

1. University of Lethbridge. Faculty of Arts and Science. Conformational analysis of DNA damaged by aristolochic acids to assess the toxicity and repair : a computational study .

Degree: 2015, University of Lethbridge

URL: http://hdl.handle.net/10133/3744

► Molecular modeling study is carried out on DNA damaged by aristolochic acids I and II. The carcinogenic effects of these acids have been attributed to… (more)

Subjects/Keywords: adenine adducts; aristolochic acid purine adducts; computational models; density functional theory; guanine adducts; mutagenesis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Science, U. o. L. F. o. A. a. (2015). Conformational analysis of DNA damaged by aristolochic acids to assess the toxicity and repair : a computational study . (Thesis). University of Lethbridge. Retrieved from http://hdl.handle.net/10133/3744

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Science, University of Lethbridge. Faculty of Arts and. “Conformational analysis of DNA damaged by aristolochic acids to assess the toxicity and repair : a computational study .” 2015. Thesis, University of Lethbridge. Accessed January 17, 2021. http://hdl.handle.net/10133/3744.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Science, University of Lethbridge. Faculty of Arts and. “Conformational analysis of DNA damaged by aristolochic acids to assess the toxicity and repair : a computational study .” 2015. Web. 17 Jan 2021.

Vancouver:

Science UoLFoAa. Conformational analysis of DNA damaged by aristolochic acids to assess the toxicity and repair : a computational study . [Internet] [Thesis]. University of Lethbridge; 2015. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/10133/3744.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Science UoLFoAa. Conformational analysis of DNA damaged by aristolochic acids to assess the toxicity and repair : a computational study . [Thesis]. University of Lethbridge; 2015. Available from: http://hdl.handle.net/10133/3744

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Vanderbilt University

2. Whitley, Brent Tyler. Aflatoxin B1 adducts with d(ATCGAT)2 and d(ATGCAT)2: Do methylation and hydroxymethylation influence reaction stoichiometry?.

Degree: MS, Chemistry, 2013, Vanderbilt University

URL: http://hdl.handle.net/1803/12018

► Aflatoxin B1 (AFB1), a carcinogenic mycotoxin produced by the common agricultural contaminant A. flavus, has been implicated in the high rates of hepatocellular carcinoma observed… (more)

Subjects/Keywords: methylation; DNA adducts; Aflatoxin; carcinogen

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Whitley, B. T. (2013). Aflatoxin B1 adducts with d(ATCGAT)2 and d(ATGCAT)2: Do methylation and hydroxymethylation influence reaction stoichiometry?. (Thesis). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/12018

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Whitley, Brent Tyler. “Aflatoxin B1 adducts with d(ATCGAT)2 and d(ATGCAT)2: Do methylation and hydroxymethylation influence reaction stoichiometry?.” 2013. Thesis, Vanderbilt University. Accessed January 17, 2021. http://hdl.handle.net/1803/12018.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Whitley, Brent Tyler. “Aflatoxin B1 adducts with d(ATCGAT)2 and d(ATGCAT)2: Do methylation and hydroxymethylation influence reaction stoichiometry?.” 2013. Web. 17 Jan 2021.

Vancouver:

Whitley BT. Aflatoxin B1 adducts with d(ATCGAT)2 and d(ATGCAT)2: Do methylation and hydroxymethylation influence reaction stoichiometry?. [Internet] [Thesis]. Vanderbilt University; 2013. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1803/12018.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Whitley BT. Aflatoxin B1 adducts with d(ATCGAT)2 and d(ATGCAT)2: Do methylation and hydroxymethylation influence reaction stoichiometry?. [Thesis]. Vanderbilt University; 2013. Available from: http://hdl.handle.net/1803/12018

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

3. Abdelhady, Rasha. Characterizing pro-mutagenic O6-alkylguanine adducts in human colorectal tissue.

Degree: 2018, University of Manchester

URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:313114

► Colorectal cancer is the third most commonly diagnosed cancer worldwide. The positive association between red and/or processed meat consumption and human colorectal cancer risk is… (more)

▼ Colorectal cancer is the third most commonly diagnosed cancer worldwide. The positive association between red and/or processed meat consumption and human colorectal cancer risk is well established potentially via heme-catalysed formation of carcinogenic N-nitrosocompounds (NNOC). These NNOC can form a range of O6- alkylguanine (O6-alkylG) adducts that may increase mutational and cancer risk. The overall aim of this study was to develop a novel approach to characterize the overall load of O6-alkylG adducts in human colorectal DNA using the known action of the DNA repair protein, O6-alkylguanine O6-alkyltransferase (MGMT) to irreversibly transfer the alkyl group from O6-alkylG to the MGMT active site peptide (MGMT-ASP). Incubation of chemically synthesised single (SS) and double stranded (DS) oligodeoxyribonucleotides (ODNs) containing O6-methylguanine (O6-MeG), O6- carboxyethylguanine (O6-CEG), but only DS O6-carboxymethylguanine (O6-CMG) -23 mer containing ODNs with MGMT resulted in MGMT inactivation. There was a preference for repair of DS ODNs. Recorded IC50 values were 0.17+0.02, 0.07+0.01, 15.7+2.1, 27.00+1.70 and 7.50+0.50 nanomolar (nM) for SS O6-MeG, DS O6-MeG, DS O6-CMG, SS O6-CEG, and DS O6-CEG, respectively. This repair was confirmed using a restriction endonuclease site deprotection assay as O6-MeG and O6-CEG inhibition of PstI digestion of ODNs, whilst, O6-CMG inhibited MboI. This inhibition was removed by pre-incubation with MGMT. Alkyl group transfer from these ODNs to a Maltose binding protein-MGMT (MBP-MGMT) fusion protein was demonstrated as alkylated active site peptides (ASPs) were detected using with Matrix assisted laser desorption/ionizationtime of flight (MALDI-TOF). S-methylcysteine (m/z 1329.7), and S-carboxyethylcysteine (m/z 1387.7) modified ASPs were detected following incubation of MBP-MGMT incubated with SS or DS O6-MeG, and O6-CEG ODNs, respectively. Scarboxymethylcysteine (m/z 1373.7) was detected with DS but not SS O6-CMG 23 mer ODN. Only the unmodified MGMT-ASP (m/z 1315.7) was found after incubating MGMT with unmodified ODN. Quantitative mass spectrometry (MS) findings highlighted that MGMT preferentially repaired specified adducts when presented in DS ODNs over SS ones. Temozolomide (TMZ) methylated calf thymus DNA (CT-DNA) and human colorectal cancer DNA samples were incubated with his tagged MGMT fusion protein (his-MGMT) and his-MGMT recovered using Ni-coated beads, digested in situ with trypsin and analysed by MALDI-TOF. MS analysis of tryptic digests of MGMT incubated with methylated CT-DNA revealed methylated MGMT-ASP, detecting as low as 50 fmole of O6-MeG/mg CT-DNA. This approach was applied to 13 human colorectal DNA samples, 12 of them showed evidence of O6 alkylation damage. Of 13 samples, 12 contained O6-MeG, 8 contained O6-CMG and 1 contained O6-HOEtG. A further 7 peaks were detected with a signal/noise (S/N) ratio of >10, potentially, correspond to alkylated MGMT-ASP ions but identities of the alkyl groups are as yet unknown. These results demonstrate proof of… Advisors/Committee Members: BARRAN, PERDITA P, Povey, Andrew, Barran, Perdita.

Subjects/Keywords: Colorectal cancer; O6-alkylguanine adducts

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Abdelhady, R. (2018). Characterizing pro-mutagenic O6-alkylguanine adducts in human colorectal tissue. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:313114

Chicago Manual of Style (16^th Edition):

Abdelhady, Rasha. “Characterizing pro-mutagenic O6-alkylguanine adducts in human colorectal tissue.” 2018. Doctoral Dissertation, University of Manchester. Accessed January 17, 2021. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:313114.

MLA Handbook (7^th Edition):

Abdelhady, Rasha. “Characterizing pro-mutagenic O6-alkylguanine adducts in human colorectal tissue.” 2018. Web. 17 Jan 2021.

Vancouver:

Abdelhady R. Characterizing pro-mutagenic O6-alkylguanine adducts in human colorectal tissue. [Internet] [Doctoral dissertation]. University of Manchester; 2018. [cited 2021 Jan 17]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:313114.

Council of Science Editors:

Abdelhady R. Characterizing pro-mutagenic O6-alkylguanine adducts in human colorectal tissue. [Doctoral Dissertation]. University of Manchester; 2018. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:313114

University of Illinois – Chicago

4. Pierce, Emily. Chemical Probe Design & Development for Assessing Protein Modification and Cross-Linking.

Degree: 2018, University of Illinois – Chicago

URL: http://hdl.handle.net/10027/22578

► Oxidative stress occurs when the production of reactive oxygen species (ROS) exceeds the capacity of antioxidants in a cell. The CNS is particularly sensitive to… (more)

▼ Oxidative stress occurs when the production of reactive oxygen species (ROS) exceeds the capacity of antioxidants in a cell. The CNS is particularly sensitive to oxidative stress and increased oxidative stress is implicated in the development and progression of neurodegenerative diseases. One of the results of increased oxidative stress is the peroxidation of polyunsaturated fatty acids (PUFAs). Lipid peroxides then undergo degradation and produce electrophilic lipid peroxidation products (LPx) which include 4-hydroxynonenal (4-HNE) and 4-oxononenal (4-ONE). These LPx contain two reactive functionalities that result in the formation of proteins adducts through different mechanisms. The aldehyde is capable of reacting with the terminal amino group of Lys residues to produce reversible Schiff base protein adducts. Additionally, the reaction of Cys, His, or Lys residues with the α,β-unsaturated aldehyde results in the formation of an irreversible Michael addition adduct. This bivalent reactivity has been shown to promote the formation of protein oligomers, implicated in neurodegenerative diseases, such as Aβ, tau, and α-synuclein. The methods commonly used to detect these protein adducts are chemical derivatization, immunoblotting, or LC-MS/MS analysis. These methods suffer from different limitations, therefore, we have developed a dual color near-IR imaging method that allows for the visualization and quantitation of the Schiff base adducts, Michael addition adducts, and protein crosslinking induced by these LPx. By adapting this method we were also able to enrich these modified proteins and use LC-MS/MS to identify HNE protein adducts in the lysates of neuronal SHSY-5Y cells. Several nucleophilic small molecules have been reported to protect tissues and cells from conditions of increased oxidative stress by behaving as chemical traps of LPx. It has also been reported that the trapping of LPx by these small molecules results in a decreased level of LPx protein adduct formation. We have shown that these trapping agents react with LPx in vitro and protect against the death of SHSY-5Y cells induced by the treatment of exogenous LPx. Our imaging method demonstrates that trapping agents containing a free thiol (glutathione, N-acetyl-cysteine) significantly decrease the formation of protein adducts. However, this method shows that the neuroprotective activity observed from certain aldehyde reactive scavengers (histidyl-hydrazide, hydralazine) is a caused by a prevention of protein cross-linking, not an overall decrease in protein adduct formation. Advisors/Committee Members: Thatcher, Gregory R.J. (advisor), Thomas, Douglas (committee member), Moore, Terry (committee member), Wang, Yue-Ting (committee member), Aldrich, Leslie (committee member), Thatcher, Gregory R.J. (chair).

Subjects/Keywords: Hydroxynonenal; protein adducts; proteomics; photoprobes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pierce, E. (2018). Chemical Probe Design & Development for Assessing Protein Modification and Cross-Linking. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/22578

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pierce, Emily. “Chemical Probe Design & Development for Assessing Protein Modification and Cross-Linking.” 2018. Thesis, University of Illinois – Chicago. Accessed January 17, 2021. http://hdl.handle.net/10027/22578.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pierce, Emily. “Chemical Probe Design & Development for Assessing Protein Modification and Cross-Linking.” 2018. Web. 17 Jan 2021.

Vancouver:

Pierce E. Chemical Probe Design & Development for Assessing Protein Modification and Cross-Linking. [Internet] [Thesis]. University of Illinois – Chicago; 2018. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/10027/22578.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pierce E. Chemical Probe Design & Development for Assessing Protein Modification and Cross-Linking. [Thesis]. University of Illinois – Chicago; 2018. Available from: http://hdl.handle.net/10027/22578

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

5. Abdelhady, Rasha. Characterizing pro-mutagenic O6-alkylguanine adducts in human colorectal tissue.

Degree: PhD, 2018, University of Manchester

URL: https://www.research.manchester.ac.uk/portal/en/theses/characterizing-promutagenic-o6alkylguanine-adducts-in-human-colorectal-tissue(61792200-cb60-425c-b667-1ebe3b2b5192).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.771360

► Colorectal cancer is the third most commonly diagnosed cancer worldwide. The positive association between red and/or processed meat consumption and human colorectal cancer risk is… (more)

▼ Colorectal cancer is the third most commonly diagnosed cancer worldwide. The positive association between red and/or processed meat consumption and human colorectal cancer risk is well established potentially via heme-catalysed formation of carcinogenic N-nitrosocompounds (NNOC). These NNOC can form a range of O6- alkylguanine (O6-alkylG) adducts that may increase mutational and cancer risk. The overall aim of this study was to develop a novel approach to characterize the overall load of O6-alkylG adducts in human colorectal DNA using the known action of the DNA repair protein, O6-alkylguanine O6-alkyltransferase (MGMT) to irreversibly transfer the alkyl group from O6-alkylG to the MGMT active site peptide (MGMT-ASP). Incubation of chemically synthesised single (SS) and double stranded (DS) oligodeoxyribonucleotides (ODNs) containing O6-methylguanine (O6-MeG), O6- carboxyethylguanine (O6-CEG), but only DS O6-carboxymethylguanine (O6-CMG) -23 mer containing ODNs with MGMT resulted in MGMT inactivation. There was a preference for repair of DS ODNs. Recorded IC50 values were 0.17+0.02, 0.07+0.01, 15.7+2.1, 27.00+1.70 and 7.50+0.50 nanomolar (nM) for SS O6-MeG, DS O6-MeG, DS O6-CMG, SS O6-CEG, and DS O6-CEG, respectively. This repair was confirmed using a restriction endonuclease site deprotection assay as O6-MeG and O6-CEG inhibition of PstI digestion of ODNs, whilst, O6-CMG inhibited MboI. This inhibition was removed by pre-incubation with MGMT. Alkyl group transfer from these ODNs to a Maltose binding protein-MGMT (MBP-MGMT) fusion protein was demonstrated as alkylated active site peptides (ASPs) were detected using with Matrix assisted laser desorption/ionizationtime of flight (MALDI-TOF). S-methylcysteine (m/z 1329.7), and S-carboxyethylcysteine (m/z 1387.7) modified ASPs were detected following incubation of MBP-MGMT incubated with SS or DS O6-MeG, and O6-CEG ODNs, respectively. Scarboxymethylcysteine (m/z 1373.7) was detected with DS but not SS O6-CMG 23 mer ODN. Only the unmodified MGMT-ASP (m/z 1315.7) was found after incubating MGMT with unmodified ODN. Quantitative mass spectrometry (MS) findings highlighted that MGMT preferentially repaired specified adducts when presented in DS ODNs over SS ones. Temozolomide (TMZ) methylated calf thymus DNA (CT-DNA) and human colorectal cancer DNA samples were incubated with his tagged MGMT fusion protein (his-MGMT) and his-MGMT recovered using Ni-coated beads, digested in situ with trypsin and analysed by MALDI-TOF. MS analysis of tryptic digests of MGMT incubated with methylated CT-DNA revealed methylated MGMT-ASP, detecting as low as 50 fmole of O6-MeG/mg CT-DNA. This approach was applied to 13 human colorectal DNA samples, 12 of them showed evidence of O6 alkylation damage. Of 13 samples, 12 contained O6-MeG, 8 contained O6-CMG and 1 contained O6-HOEtG. A further 7 peaks were detected with a signal/noise (S/N) ratio of >10, potentially, correspond to alkylated MGMT-ASP ions but identities of the alkyl groups are as yet unknown. These results demonstrate proof of…

Subjects/Keywords: O6-alkylguanine adducts; Colorectal cancer

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APA (6^th Edition):

Abdelhady, R. (2018). Characterizing pro-mutagenic O6-alkylguanine adducts in human colorectal tissue. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/characterizing-promutagenic-o6alkylguanine-adducts-in-human-colorectal-tissue(61792200-cb60-425c-b667-1ebe3b2b5192).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.771360

Chicago Manual of Style (16^th Edition):

Abdelhady, Rasha. “Characterizing pro-mutagenic O6-alkylguanine adducts in human colorectal tissue.” 2018. Doctoral Dissertation, University of Manchester. Accessed January 17, 2021. https://www.research.manchester.ac.uk/portal/en/theses/characterizing-promutagenic-o6alkylguanine-adducts-in-human-colorectal-tissue(61792200-cb60-425c-b667-1ebe3b2b5192).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.771360.

MLA Handbook (7^th Edition):

Abdelhady, Rasha. “Characterizing pro-mutagenic O6-alkylguanine adducts in human colorectal tissue.” 2018. Web. 17 Jan 2021.

Vancouver:

Abdelhady R. Characterizing pro-mutagenic O6-alkylguanine adducts in human colorectal tissue. [Internet] [Doctoral dissertation]. University of Manchester; 2018. [cited 2021 Jan 17]. Available from: https://www.research.manchester.ac.uk/portal/en/theses/characterizing-promutagenic-o6alkylguanine-adducts-in-human-colorectal-tissue(61792200-cb60-425c-b667-1ebe3b2b5192).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.771360.

Council of Science Editors:

Abdelhady R. Characterizing pro-mutagenic O6-alkylguanine adducts in human colorectal tissue. [Doctoral Dissertation]. University of Manchester; 2018. Available from: https://www.research.manchester.ac.uk/portal/en/theses/characterizing-promutagenic-o6alkylguanine-adducts-in-human-colorectal-tissue(61792200-cb60-425c-b667-1ebe3b2b5192).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.771360

Latrobe University

6. Ugarenko, Michal. Exploiting anthracycline-dna adduct formation to enhance anthracycline anticancer activity.

Degree: PhD, 2012, Latrobe University

URL: http://hdl.handle.net/1959.9/518141

►

Thesis (Ph.D.) - La Trobe University, 2012

Submission note: "A thesis submitted in total fulfillment of the requirements for the degree of Doctor of Philosophy… (more)

Subjects/Keywords: Chemotherapy.; DNA adducts.; Anthracyclines.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ugarenko, M. (2012). Exploiting anthracycline-dna adduct formation to enhance anthracycline anticancer activity. (Doctoral Dissertation). Latrobe University. Retrieved from http://hdl.handle.net/1959.9/518141

Chicago Manual of Style (16^th Edition):

Ugarenko, Michal. “Exploiting anthracycline-dna adduct formation to enhance anthracycline anticancer activity.” 2012. Doctoral Dissertation, Latrobe University. Accessed January 17, 2021. http://hdl.handle.net/1959.9/518141.

MLA Handbook (7^th Edition):

Ugarenko, Michal. “Exploiting anthracycline-dna adduct formation to enhance anthracycline anticancer activity.” 2012. Web. 17 Jan 2021.

Vancouver:

Ugarenko M. Exploiting anthracycline-dna adduct formation to enhance anthracycline anticancer activity. [Internet] [Doctoral dissertation]. Latrobe University; 2012. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1959.9/518141.

Council of Science Editors:

Ugarenko M. Exploiting anthracycline-dna adduct formation to enhance anthracycline anticancer activity. [Doctoral Dissertation]. Latrobe University; 2012. Available from: http://hdl.handle.net/1959.9/518141

University of Akron

7. Johnson, Nicholas A. Phosphazenes: From Polymer to Superbase.

Degree: PhD, Chemistry, 2015, University of Akron

URL: http://rave.ohiolink.edu/etdc/view?acc_num=akron1436517844

► Polyphosphazenes represent the largest class of inorganic backbone polymers and a wide array of applications exists and continues to accumulate. Although many useful phosphazene polymer… (more)

▼ Polyphosphazenes represent the largest class of inorganic backbone polymers and a wide array of applications exists and continues to accumulate. Although many useful phosphazene polymer systems exist, commercial use has been limited due to irreproducibilities and high cost of the parent polymer, polydichlorophosphazene, from which most other polyphosphazenes are derived. Although the ring-opening polymerization of [PCl2N]3 has been extensively studied since the mid-1960s, the mechanism is still under much debate and the search for novel initiators to the process is ongoing. The majority of phosphazenes are derived from the chlorophosphazenes; however, the properties of the P-N backbone of phosphazenes can be greatly tuned with the addition of different side groups attached to the phosphorus atom. A large variety of applications for substituted phosphazene compounds have been developed ranging from biological materials that are water-degradable to phosphazene superbases that have been used as initiators for several organic polymerizations.The main focus of this dissertation is fundamental phosphazene chemistry ranging from investigations of the initiating steps of the ring-opening polymerization of [PCl2N]3 to interactions of phosphazene superbases with classic Lewis acids. This dissertation is divided into six chapters: introduction, interactions of phosphazene superbases with group 1 and group 12 Lewis acids, interactions of phosphazene superbases with group 13 Lewis acids, reaction of phosphazene superbases with [PCl2N]3, phosphazenes for biological applications, and conclusion. Chapter I provides an overall review of polyphosphazene synthesis including initiators and mechanistic discussions as well as use of phosphazene superbases as frustrated Lewis pairs and initiators in anionic ring-opening polymerizations. Chapter II contains explorations of phosphazene superbases and their interactions with group 1 and group 12 Lewis acids. Chapter III is an investigation of phosphazene superbases with Group 13 Lewis acids and a brief investigation into these complexes frustrated Lewis pair (FLP) capabilities. Chapter IV explores the interactions of phosphazene superbases with cyclic chlorophosphazene trimer ([PCl2N]3) and the investigation of a tadpole-like structure that is formed, similar to the complex that is implicated as the initiating species to ring-opening polymerization. Chapter V investigates the utility of ethylene glycol substituted [PCl2N]3 as a phosphazene-based drug delivery system including synthesis and purification of stereoisomers. Chapter VI contains the conclusions of this dissertation. Advisors/Committee Members: Tessier, Claire (Advisor).

Subjects/Keywords: Chemistry; Inorganic Chemistry; phosphazene; phosphazene superbase; inorganic polymers; group 13 adducts; group 1 adducts; group 12 adducts; phosphonitrlic chloride; tadpole

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Johnson, N. A. (2015). Phosphazenes: From Polymer to Superbase. (Doctoral Dissertation). University of Akron. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=akron1436517844

Chicago Manual of Style (16^th Edition):

Johnson, Nicholas A. “Phosphazenes: From Polymer to Superbase.” 2015. Doctoral Dissertation, University of Akron. Accessed January 17, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=akron1436517844.

MLA Handbook (7^th Edition):

Johnson, Nicholas A. “Phosphazenes: From Polymer to Superbase.” 2015. Web. 17 Jan 2021.

Vancouver:

Johnson NA. Phosphazenes: From Polymer to Superbase. [Internet] [Doctoral dissertation]. University of Akron; 2015. [cited 2021 Jan 17]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron1436517844.

Council of Science Editors:

Johnson NA. Phosphazenes: From Polymer to Superbase. [Doctoral Dissertation]. University of Akron; 2015. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron1436517844

Vanderbilt University

8. Stavros, Kallie Marie. Conformation of the Aryl Amine DNA Adduct 2-Amino-3-methylimidazole-[4,5-f]quinoline N2-deoxyguanine in the NarI Endonuclease Recognition Sequence as Determined by NMR Spectroscopy.

Degree: PhD, Chemical and Physical Biology, 2015, Vanderbilt University

URL: http://hdl.handle.net/1803/10556

► 2-Amino-3-methylimidazo-[4,5-f]quinoline, IQ, has been identified as one of the most genotoxic materials according to Ames assays and animal studies. IQ is during high temperature cooking… (more)

▼ 2-Amino-3-methylimidazo-[4,5-f]quinoline, IQ, has been identified as one of the most genotoxic materials according to Ames assays and animal studies. IQ is during high temperature cooking of meats and is classified as probably human carcinogen by the IARC. The N2-dG adduct has been shown to have a sequence dependence when replicated by Polη when located in a GCGC repeat sequence. This project investigates differences between the adduct located at the G1 position of the 12mer containing the NarI recognition sequence (G1G2CG3CC) as well as the mutagenic hotspot G3 position using high-field solution NMR. Overall, NMR data supports both adducts adopt a base-displaced intercalated in the duplex, where the IQ spans across the duplex and stacks with the flanking base pairs. The modified bases are displaced into the major groove, while the base opposite the modification is completely flipped into the major groove. Both modified bases maintain the anti conformation. Both adducts do not change the thermal stability of the duplex. The IQ moiety at G3 position, however, is angled by 15° relative to the X7 base, whereas the IQ at G1 remains planar to X4. Further studies are needed to determine if and how this might be significant during replication, but if the orientation is maintained in the polymerase active site, it could play a role in the context determining mutagenicity. This project also differentiates the N2-dG adduct from the major C8-dG adduct, where the N2-dG-IQ has been observed to be more persistent in tissues and provides insight to how it might evade repair by the nucleotide excision repair pathway. This likely relates to the ability of IQ to participate in base-stacking in the duplex when attached at the N2 position of guanine as well as how the modified base remains in the anti conformation. The C8-dG-IQ, however, changes to the syn conformation. The orientation of the C8-dG-IQ results in destabilizing perturbations to the helix that are reflected in the thermal stabilities. These differences could likely contribute to the differing repair response for these IQ adducts. Advisors/Committee Members: Michael Stone (committee member), Carmelo Rizzo (committee member), Brandt Eichman (committee member), Charles Sanders (committee member), Frederick Guengerich (committee member).

Subjects/Keywords: structural biology; NMR; DNA adducts; heterocyclic amines

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APA (6^th Edition):

Stavros, K. M. (2015). Conformation of the Aryl Amine DNA Adduct 2-Amino-3-methylimidazole-[4,5-f]quinoline N2-deoxyguanine in the NarI Endonuclease Recognition Sequence as Determined by NMR Spectroscopy. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/10556

Chicago Manual of Style (16^th Edition):

Stavros, Kallie Marie. “Conformation of the Aryl Amine DNA Adduct 2-Amino-3-methylimidazole-[4,5-f]quinoline N2-deoxyguanine in the NarI Endonuclease Recognition Sequence as Determined by NMR Spectroscopy.” 2015. Doctoral Dissertation, Vanderbilt University. Accessed January 17, 2021. http://hdl.handle.net/1803/10556.

MLA Handbook (7^th Edition):

Stavros, Kallie Marie. “Conformation of the Aryl Amine DNA Adduct 2-Amino-3-methylimidazole-[4,5-f]quinoline N2-deoxyguanine in the NarI Endonuclease Recognition Sequence as Determined by NMR Spectroscopy.” 2015. Web. 17 Jan 2021.

Vancouver:

Stavros KM. Conformation of the Aryl Amine DNA Adduct 2-Amino-3-methylimidazole-[4,5-f]quinoline N2-deoxyguanine in the NarI Endonuclease Recognition Sequence as Determined by NMR Spectroscopy. [Internet] [Doctoral dissertation]. Vanderbilt University; 2015. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1803/10556.

Council of Science Editors:

Stavros KM. Conformation of the Aryl Amine DNA Adduct 2-Amino-3-methylimidazole-[4,5-f]quinoline N2-deoxyguanine in the NarI Endonuclease Recognition Sequence as Determined by NMR Spectroscopy. [Doctoral Dissertation]. Vanderbilt University; 2015. Available from: http://hdl.handle.net/1803/10556

University of Hyderabad

9. Kunche, Aravindu. Towards development of novel strategies for synthesis of fused heterocyclic and carbocyclic frameworks and himanimide a using baylis hillman adducts; -.

Degree: Chemistry, 2010, University of Hyderabad

URL: http://shodhganga.inflibnet.ac.in/handle/10603/4219

►

The Baylis-Hillman reaction is an atom-economy three component carbon-carbon bond forming reaction involving the coupling of and#945;-position of activated alkene with an electrophile under the… (more)

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The Baylis-Hillman reaction is an atom-economy three component carbon-carbon bond forming reaction involving the coupling of and#945;-position of activated alkene with an electrophile under the influence of a catalyst / catalytic system producing interesting and useful classes of highly functionalized molecules. These multifunctional molecules, usually known as Baylis-Hillman adducts, have been elegantly employed in various organic transformation methodologies and also in the synthesis of biologically active molecules and natural products. Our research group has been working on various aspects of this fascinating reaction for the last several years with the main objective of developing Baylis-Hillman adducts as a valuable source for the synthesis of various heterocyclic and carbocyclic molecules in a one-pot multistep reaction strategy. This thesis deals with applications of Baylis-Hillman adducts in synthesis of heterocyclic / carbocyclic molecules and also synthesis of himanimide A and consists of three chapters 1) Introduction 2) Objectives, Results and Discussion and 3) Experimental. The first chapter, i.e., Introduction presents a brief literature survey on the recent and relevant developments in the Baylis?Hillman reaction and also on applications of the Baylis?Hillman adducts in synthetic organic chemistry. The second chapter deals with the development of simple and convenient methodologies for synthesis of heterocyclic / carbocyclic molecules from the Baylis-Hillman adducts with the following main objectives. OBJECTIVE 1) To develop a facile methodology for synthesis of functionalized tri-/tetracyclic frameworks containing azocine moiety as one of the central rings using the Baylis-Hillman acetates as the starting materials. 2) To develop a simple, one-pot, and facile synthetic strategy for synthesis of angularly fused [6-7-5], [6-7-6], [6-7-7] and [6,7] carbocyclic ring systems containing cycloheptane as the central ring using the Baylis-Hillman acetates as the starting materials.

References p.199-214

Advisors/Committee Members: Basavaiah, D.

Subjects/Keywords: Chemistry; Baylis Hillman Adducts; Fused Heterocyclic

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APA (6^th Edition):

Kunche, A. (2010). Towards development of novel strategies for synthesis of fused heterocyclic and carbocyclic frameworks and himanimide a using baylis hillman adducts; -. (Thesis). University of Hyderabad. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/4219

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kunche, Aravindu. “Towards development of novel strategies for synthesis of fused heterocyclic and carbocyclic frameworks and himanimide a using baylis hillman adducts; -.” 2010. Thesis, University of Hyderabad. Accessed January 17, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/4219.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kunche, Aravindu. “Towards development of novel strategies for synthesis of fused heterocyclic and carbocyclic frameworks and himanimide a using baylis hillman adducts; -.” 2010. Web. 17 Jan 2021.

Vancouver:

Kunche A. Towards development of novel strategies for synthesis of fused heterocyclic and carbocyclic frameworks and himanimide a using baylis hillman adducts; -. [Internet] [Thesis]. University of Hyderabad; 2010. [cited 2021 Jan 17]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/4219.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kunche A. Towards development of novel strategies for synthesis of fused heterocyclic and carbocyclic frameworks and himanimide a using baylis hillman adducts; -. [Thesis]. University of Hyderabad; 2010. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/4219

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Minnesota

10. Warnert, Marissa A. Modulation of prostate and pancreatic PhIP-DNA adducts by consumption of cruciferous and apiaceous vegetables in male wistar rats.

Degree: MS, Nutrition, 2013, University of Minnesota

URL: http://purl.umn.edu/157252

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University of Minnesota M.S. thesis. May 2013. Major: Nutrition. Advisor: Sabrina P. Trudo. 1 computer file (PDF); viii, 131 pages, appendices A-B.

PhIP (2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine )… (more)

Subjects/Keywords: Adducts; Apiaceous; Cruciferous; Pancreas; PhIP-DNA; Prostate

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APA (6^th Edition):

Warnert, M. A. (2013). Modulation of prostate and pancreatic PhIP-DNA adducts by consumption of cruciferous and apiaceous vegetables in male wistar rats. (Masters Thesis). University of Minnesota. Retrieved from http://purl.umn.edu/157252

Chicago Manual of Style (16^th Edition):

Warnert, Marissa A. “Modulation of prostate and pancreatic PhIP-DNA adducts by consumption of cruciferous and apiaceous vegetables in male wistar rats.” 2013. Masters Thesis, University of Minnesota. Accessed January 17, 2021. http://purl.umn.edu/157252.

MLA Handbook (7^th Edition):

Warnert, Marissa A. “Modulation of prostate and pancreatic PhIP-DNA adducts by consumption of cruciferous and apiaceous vegetables in male wistar rats.” 2013. Web. 17 Jan 2021.

Vancouver:

Warnert MA. Modulation of prostate and pancreatic PhIP-DNA adducts by consumption of cruciferous and apiaceous vegetables in male wistar rats. [Internet] [Masters thesis]. University of Minnesota; 2013. [cited 2021 Jan 17]. Available from: http://purl.umn.edu/157252.

Council of Science Editors:

Warnert MA. Modulation of prostate and pancreatic PhIP-DNA adducts by consumption of cruciferous and apiaceous vegetables in male wistar rats. [Masters Thesis]. University of Minnesota; 2013. Available from: http://purl.umn.edu/157252

University of Minnesota

11. Warnert, Marissa A. Modulation of prostate and pancreatic PhIP-DNA adducts by consumption of cruciferous and apiaceous vegetables in male wistar rats.

Degree: MS, Nutrition, 2013, University of Minnesota

URL: http://purl.umn.edu/157252

► PhIP (2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine ) is one of the most abundant heterocylic aromatic amines (HAA), which are dietary procarcinogens in over-cooked meat. Some phase I biotransformation enzymes… (more)

Subjects/Keywords: Adducts; Apiaceous; Cruciferous; Pancreas; PhIP-DNA; Prostate

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Warnert, M. A. (2013). Modulation of prostate and pancreatic PhIP-DNA adducts by consumption of cruciferous and apiaceous vegetables in male wistar rats. (Masters Thesis). University of Minnesota. Retrieved from http://purl.umn.edu/157252

Chicago Manual of Style (16^th Edition):

Warnert, Marissa A. “Modulation of prostate and pancreatic PhIP-DNA adducts by consumption of cruciferous and apiaceous vegetables in male wistar rats.” 2013. Masters Thesis, University of Minnesota. Accessed January 17, 2021. http://purl.umn.edu/157252.

MLA Handbook (7^th Edition):

Warnert, Marissa A. “Modulation of prostate and pancreatic PhIP-DNA adducts by consumption of cruciferous and apiaceous vegetables in male wistar rats.” 2013. Web. 17 Jan 2021.

Vancouver:

Warnert MA. Modulation of prostate and pancreatic PhIP-DNA adducts by consumption of cruciferous and apiaceous vegetables in male wistar rats. [Internet] [Masters thesis]. University of Minnesota; 2013. [cited 2021 Jan 17]. Available from: http://purl.umn.edu/157252.

Council of Science Editors:

Warnert MA. Modulation of prostate and pancreatic PhIP-DNA adducts by consumption of cruciferous and apiaceous vegetables in male wistar rats. [Masters Thesis]. University of Minnesota; 2013. Available from: http://purl.umn.edu/157252

University of Guelph

12. Verwey, Anne. In vitro Insight toward the Mutagenicity of Carbon- and Oxygen-Linked C8-Aryl-Deoxyguanosine Adducts.

Degree: MS, Department of Chemistry, 2015, University of Guelph

URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8723

► Aryl radicals generated through metabolism of polyaromatic hydrocarbons and arylhydrazines can react directly at the C8-site of 2’-deoxyguanosine (dG) to form carbon (C)-linked C8-dG adducts.… (more)

Subjects/Keywords: DNA damage; DNA; DNA adducts; DNA replication; Mutagenicity; Deoxyguanosine; Klenow fragment; Translesion polymerase; C8-aryl-dG adducts

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Verwey, A. (2015). In vitro Insight toward the Mutagenicity of Carbon- and Oxygen-Linked C8-Aryl-Deoxyguanosine Adducts. (Masters Thesis). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8723

Chicago Manual of Style (16^th Edition):

Verwey, Anne. “In vitro Insight toward the Mutagenicity of Carbon- and Oxygen-Linked C8-Aryl-Deoxyguanosine Adducts.” 2015. Masters Thesis, University of Guelph. Accessed January 17, 2021. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8723.

MLA Handbook (7^th Edition):

Verwey, Anne. “In vitro Insight toward the Mutagenicity of Carbon- and Oxygen-Linked C8-Aryl-Deoxyguanosine Adducts.” 2015. Web. 17 Jan 2021.

Vancouver:

Verwey A. In vitro Insight toward the Mutagenicity of Carbon- and Oxygen-Linked C8-Aryl-Deoxyguanosine Adducts. [Internet] [Masters thesis]. University of Guelph; 2015. [cited 2021 Jan 17]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8723.

Council of Science Editors:

Verwey A. In vitro Insight toward the Mutagenicity of Carbon- and Oxygen-Linked C8-Aryl-Deoxyguanosine Adducts. [Masters Thesis]. University of Guelph; 2015. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8723

13. Lin, De. Modification and Cross-Linking of Proteins by Lipoxidation Derived Electrophiles: Chemical and Biological Consequences.

Degree: PhD, Chemistry, 2007, Case Western Reserve University School of Graduate Studies

URL: http://rave.ohiolink.edu/etdc/view?acc_num=case1166813930

► Chemical modification of proteins and polynucleotides by electrophilic products of lipid oxidation, generated under condition of oxidative stress, have been implicated in a variety… (more)

▼ Chemical modification of proteins and polynucleotides by electrophilic products of lipid oxidation, generated under condition of oxidative stress, have been implicated in a variety of diseases, such as atherosclerosis, neurodegenerative disease, liver disease, and cancer, to name a few. Most work has focused on reactive bifunctional aldehydes like malondialdehyde, 4-hydroxy-2-nonenal (HNE), 4-oxo-2-nonenal (ONE), and 4,5-epoxy-2-alkenals, in each case with an eye on modification and cross-linking of protein-based lysine, histidine, and cysteine residues. The reactions of HNE and ONE with two model proteins, RNase and β-lactoglobulin, and their Lys ε-dimethylamino derivatives, studied through spectrophotometry, showed that ONE was 6-31 times more reactive than HNE with these proteins. The fastest reaction of ONE with proteins involves Schiff base formation at Lys ε-amino groups, whereas Schiff base formation is not spectroscopically apparent for HNE. Detailed kinetic studies of the initial reaction of HNE and ONE were carried out with amino acids and amino acid surrogates. Whereas the reactions with imidazole and thiol nucleophiles involve straightforward Michael adduct formation, kinetics analyses revealed the reversibility of both HNE Michael addition to amine and ONE Schiff base formation with amine. Although ONE is more reactive than HNE toward conjugate addition of imidazole and thiol nucleophiles, it is less reactive than HNE toward Lys/amine Michael adduction. Moreover, ONE is 4-5 times more cytotoxic than HNE to human neuroblastoma cells at concentrations near the threshold of lethality. The greater neurotoxicity of ONE could reflect in part the different reactivity characteristics of ONE as compared to HNE. Mass spectrometric studies (MALDI-TOF, HPLC-MS/MS) revealed that apomyoglobin was modified predominantly by isomeric epoxyketooctadecenoic acid (EKODE) residues when the protein was exposed to mild non-enzymatic oxidation of linoleic acid. Six regio- and stereoismoers of EKODE were independently synthesized and identified by HPLC in the system of autoxidation of linoleic acid catalyzed by Fe(II)/ascorbic acid. The nature of EKODE-His adduct formation was defined through model studies to reflect Michael addition of the His imidazole group. In addition, kinetic studies revealed that the various EKODE isomeric families show significantly different reactivity toward imidazole nucleophiles. 4,5-Epoxy-2-alkenals, another family of lipoxidation end products, can modify proteins and give rise to Lys-Lys protein cross-linking in the form of lipofuscin-like polypyrroles. Here a novel Lys-His cross-linking piperidine structure was identified in the reaction of <i>trans</i>-4,5-epoxy-2(<i>E</i>)-heptenal (EH) with simple amine and imidazole surrogates for the Lys and His side-chains of proteins. Protein cross-linking studies indicated that Lys-His cross-linking should play a more important role than Lys-Lys cross-linking reported before. Neurofilament proteins (NFs) are among the major… Advisors/Committee Members: Pearson, Anthony (Committee Chair).

Subjects/Keywords: Chemistry, Biochemistry; HNE; EKODE; 1H; Adducts; Hz; Michael Adducts

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lin, D. (2007). Modification and Cross-Linking of Proteins by Lipoxidation Derived Electrophiles: Chemical and Biological Consequences. (Doctoral Dissertation). Case Western Reserve University School of Graduate Studies. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=case1166813930

Chicago Manual of Style (16^th Edition):

Lin, De. “Modification and Cross-Linking of Proteins by Lipoxidation Derived Electrophiles: Chemical and Biological Consequences.” 2007. Doctoral Dissertation, Case Western Reserve University School of Graduate Studies. Accessed January 17, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=case1166813930.

MLA Handbook (7^th Edition):

Lin, De. “Modification and Cross-Linking of Proteins by Lipoxidation Derived Electrophiles: Chemical and Biological Consequences.” 2007. Web. 17 Jan 2021.

Vancouver:

Lin D. Modification and Cross-Linking of Proteins by Lipoxidation Derived Electrophiles: Chemical and Biological Consequences. [Internet] [Doctoral dissertation]. Case Western Reserve University School of Graduate Studies; 2007. [cited 2021 Jan 17]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=case1166813930.

Council of Science Editors:

Lin D. Modification and Cross-Linking of Proteins by Lipoxidation Derived Electrophiles: Chemical and Biological Consequences. [Doctoral Dissertation]. Case Western Reserve University School of Graduate Studies; 2007. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=case1166813930

14. Santos, Isabela Ferreira Sodré dos. Investigação da correlação morfologia/estrutura nos produtos da polimerização da anilina com persulfato de amônio.

Degree: Mestrado, Química, 2013, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/46/46136/tde-22102013-104923/ ;

►

É verificado que materiais nano/micro estruturado, com morfologia definida possui propriedades melhoradas em relação às formas macro. Essa constatação justifica o crescente número de rotas… (more)

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É verificado que materiais nano/micro estruturado, com morfologia definida possui propriedades melhoradas em relação às formas macro. Essa constatação justifica o crescente número de rotas diferentes para se sintetizar polianilina numa ampla gama de morfologias diferentes e tamanhos definidos, através de variações na síntese padrão de polianilina. Utilizando uma dessas rotas que leva a produto com morfologia esférica, em estudo anterior do nosso grupo foi mostrado que o produto principal é adutos de anilinas constituídos por adutos tipo 1,4-Michael entre anilina e benzoquinona monoimina e segmentos fenazínicos substituídos. Neste trabalho foram feitas modificações nesse protocolo de síntese e os produtos foram caracterizados tanto morfologicamente como estruturalmente. A morfologia dos produtos foi determinada por microscopia eletrônica de varredura enquanto que as técnicas UV-VIS, Raman, IR e Fluorescência forneceram a estrutura dos produtos. A oxidação de anilina por persulfato de amônio sob sete diferentes condições experimentais foram realizadas mantendo o pH da solução reacional constante em 3 ou 4.5. Os produtos das condições experimentais realizadas apresentaram morfologias tipo esféricas (microesferas lisas ou porosas, e microesferas Tipo-Folha) e foram caracterizados como adutos de anilina constituídos por adutos tipo 1,4-Michael e segmentos fenazínicos não substituídos como produto majoritário de síntese. O grau de hidrólise dos nitrogênios imínicos mostrou dependência com o pH de síntese e concentrações dos reagentes. Os resultados sugeriram que o grau hidrólise dos nitrogênios imínicos é um fator importante na determinação de estruturas esféricas, assim como as concentrações dos reagentes, uma vez que estes aspectos podem influenciar na capacidade de interação entre os adutos de anilina que resulta na formação destas estruturas. A verificação de segmentos fenazínicos não substituídos (diferente do verificado no estudo anterior) na constituição dos adutos de anilina chama a atenção para a importância de caracterizar estes segmentos pela técnica de espectroscopia de Fluorescência nos trabalhos anteriores do grupo. A formação de produto de polimerização cabeça-cauda (PANI-ES) foi verificada na condição experimental realizada em pH 4,5 mais concentrada e nas condições experimentais realizadas em pH 3, exceto na condição mais diluída, corroborando a afirmação de que, além do pH reacional, a concentração absoluta dos reagentes influi na determinação do produto final

It is known that nano/micro structured materials with defined morphology has improved properties when compared to bulk forms. This finding justifies the increasing number of different routes to synthesize polyaniline in a wide range of different morphologies and sizes defined by variations in the standard synthesis of polyaniline. Using one of these routes that leads to product with spherical morphology, in a previous study from our group it was shown that the main product is aniline adducts formed by 1,4-Michael type adducts between aniline and…

Advisors/Committee Members: Temperini, Marcia Laudelina Arruda.

Subjects/Keywords: Adutos de anilina; Aniline adducts; Espectrometria; Polianilina; Polyaniline; Raman; Raman; Spectrometry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Santos, I. F. S. d. (2013). Investigação da correlação morfologia/estrutura nos produtos da polimerização da anilina com persulfato de amônio. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/46/46136/tde-22102013-104923/ ;

Chicago Manual of Style (16^th Edition):

Santos, Isabela Ferreira Sodré dos. “Investigação da correlação morfologia/estrutura nos produtos da polimerização da anilina com persulfato de amônio.” 2013. Masters Thesis, University of São Paulo. Accessed January 17, 2021. http://www.teses.usp.br/teses/disponiveis/46/46136/tde-22102013-104923/ ;.

MLA Handbook (7^th Edition):

Santos, Isabela Ferreira Sodré dos. “Investigação da correlação morfologia/estrutura nos produtos da polimerização da anilina com persulfato de amônio.” 2013. Web. 17 Jan 2021.

Vancouver:

Santos IFSd. Investigação da correlação morfologia/estrutura nos produtos da polimerização da anilina com persulfato de amônio. [Internet] [Masters thesis]. University of São Paulo; 2013. [cited 2021 Jan 17]. Available from: http://www.teses.usp.br/teses/disponiveis/46/46136/tde-22102013-104923/ ;.

Council of Science Editors:

Santos IFSd. Investigação da correlação morfologia/estrutura nos produtos da polimerização da anilina com persulfato de amônio. [Masters Thesis]. University of São Paulo; 2013. Available from: http://www.teses.usp.br/teses/disponiveis/46/46136/tde-22102013-104923/ ;

15. Dutra, Igo. Investigação de lesões em DNA induzidas pelo hidrocarboneto policíclico aromático antantreno.

Degree: Mestrado, Toxicologia e Análises Toxicológicas, 2007, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/9/9141/tde-07122007-112557/ ;

►

O antantreno é um hidrocarboneto hexacíclico aromático (HPA) bastante difundido no meio ambiente e que não apresenta as regiões de baía ou fjord, que em… (more)

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O antantreno é um hidrocarboneto hexacíclico aromático (HPA) bastante difundido no meio ambiente e que não apresenta as regiões de baía ou fjord, que em geral, conferem alta reatividade a esses compostos após oxidação. Entretanto, sua mutagenicidade em bactérias após ativação metabólica é comparável à do Benzopireno (BP) e, com base na sensibilidade das linhagens de S. typhimurium utilizadas, os metabólitos do antantreno causam substituições de pares de base, trocas de fita e possivelmente dano oxidativo ao DNA. Alguns dos metabólitos que apresentam ação genotóxica em bactérias foram recentemente identificados, mas uma investigação mais ampla considerando a ligação desses e outros possíveis metabólitos a biomoléculas, com a identificação dos adutos formados, pode contribuir para um melhor esclarecimento das vias envolvidas em sua genotoxicidade. Neste trabalho investigamos a reação do antantreno (oxidado quimicamente e pelo sistema HRP/H2O2) com dG e DNA in vitro, tendo sido observada a formação de possíveis adutos nas análises por HPLC/ESI/MS, HPLC/UV/Fluorescência e espectrofluorimetria. Células de carcinoma hepatocelular humano (HepG2) e hepatócitos de fígado humano normal (THLE-2) foram incubadas com antantreno e seus produtos de oxidação (quinonas e hidroquinonas acetiladas), verificando-se citotoxicidade dose dependente em diferentes condições de cultura. Uma vez que a sobrevivência relativa das células THLE-2 cultivadas sobre filme de colágeno e em meio PFMR-4 se assemelhou à sobrevivência relativa das células HepG2 cultivadas em meio DME e sem filme de colágeno, utilizamos as células HepG2 para análise de dano oxidativo. Produtos de oxidação do antantreno (quinonas e hidroquinonas acetiladas) induziram a formação de 8-oxodGuo no DNA celular. Os dados obtidos nos indicam que duas vias podem estar envolvidas na genotoxicidade do antantreno observada nos estudos com S.typhimurium realizados por Platt et al. (2002): indução de dano oxidativo e formação de adutos com o DNA.

Anthanthrene is a polycyclic aromatic hydrocarbon (PAH) widely spread in the environment and does not have the bay or fjord regions, which in general provide high reactivity to these compounds after oxidation. However, its mutagenic activity to bacteria after metabolic activation is comparable to that of benzopyrene (BP) and, based on the sensitivity of the S. typhimurium strains used, anthanthrene metabolites cause base pair substitutions, DNA strand exchanges, and possibly DNA oxidative damage. Some of the metabolites that are genotoxic to bacteria have been recently identified, but a better investigation on how these and other possible metabolites react with biomolecules, identifying the generated adducts, can contribute to clarify the ways involved in its genotoxicity. In the present work we investigated the reaction of anthanthrene (chemically oxidized or oxidized by the HRP/H2O2 system) with dG and DNA in vitro. The formation of possible adducts was observed in the analyses by HPLC/ESI/MS, HPLC/UV/Fluorescence and…

Advisors/Committee Members: Loureiro, Ana Paula de Melo.

Subjects/Keywords: Adducts.; Adutos.; DNA; DNA; Estresse oxidativo; Lesions; Lesões; Oxidative stress

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dutra, I. (2007). Investigação de lesões em DNA induzidas pelo hidrocarboneto policíclico aromático antantreno. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/9/9141/tde-07122007-112557/ ;

Chicago Manual of Style (16^th Edition):

Dutra, Igo. “Investigação de lesões em DNA induzidas pelo hidrocarboneto policíclico aromático antantreno.” 2007. Masters Thesis, University of São Paulo. Accessed January 17, 2021. http://www.teses.usp.br/teses/disponiveis/9/9141/tde-07122007-112557/ ;.

MLA Handbook (7^th Edition):

Dutra, Igo. “Investigação de lesões em DNA induzidas pelo hidrocarboneto policíclico aromático antantreno.” 2007. Web. 17 Jan 2021.

Vancouver:

Dutra I. Investigação de lesões em DNA induzidas pelo hidrocarboneto policíclico aromático antantreno. [Internet] [Masters thesis]. University of São Paulo; 2007. [cited 2021 Jan 17]. Available from: http://www.teses.usp.br/teses/disponiveis/9/9141/tde-07122007-112557/ ;.

Council of Science Editors:

Dutra I. Investigação de lesões em DNA induzidas pelo hidrocarboneto policíclico aromático antantreno. [Masters Thesis]. University of São Paulo; 2007. Available from: http://www.teses.usp.br/teses/disponiveis/9/9141/tde-07122007-112557/ ;

Vanderbilt University

16. Mitchener, Michelle Marie. A. Competition and allostery govern substrate selectivity of cyclooxygenase-2 B. Enzymatic oxidation of M1dG in the genome.

Degree: PhD, Chemistry, 2017, Vanderbilt University

URL: http://hdl.handle.net/1803/15119

► Part A: Cyclooxygenase-2 (COX-2) oxygenates arachidonic acid (AA) and its ester analog, 2-arachidonoylglycerol (2-AG), to prostaglandins (PGs) and prostaglandin glyceryl esters (PG-Gs), respectively. Although the… (more)

▼ Part A: Cyclooxygenase-2 (COX-2) oxygenates arachidonic acid (AA) and its ester analog, 2-arachidonoylglycerol (2-AG), to prostaglandins (PGs) and prostaglandin glyceryl esters (PG-Gs), respectively. Although the efficiency of oxygenation of these substrates by COX-2 in vitro is similar, cellular biosynthesis of PGs far exceeds that of PG-Gs. Evidence that the homodimeric COX enzymes are functional heterodimers comprising a catalytic and an allosteric subunit suggests that competitive interaction of AA and 2-AG at the allosteric site of COX-2 might result in differential regulation of the oxygenation of the two substrates when both are present. Modulation of AA levels in RAW264.7 macrophages uncovered an inverse correlation between cellular AA levels and PG-G biosynthesis. In vitro kinetic analysis using purified protein demonstrated that the inhibition of 2-AG oxygenation by high concentrations of AA far exceeded the inhibition of AA oxygenation by high concentrations of 2-AG. An unbiased systems-based mechanistic model of the kinetic data revealed that binding of AA or 2-AG at the allosteric site of COX-2 results in a decreased catalytic efficiency of the enzyme toward 2-AG, whereas 2-AG binding at the allosteric site increases COX-2's efficiency toward AA. The results suggest that substrates interact with COX-2 via multiple potential complexes involving binding to both the catalytic and allosteric sites. Competition between AA and 2-AG for these sites, combined with differential allosteric modulation, gives rise to a complex interplay between the substrates, leading to preferential oxygenation of AA. Part B: Lipid and DNA peroxidation give rise to DNA adducts, the most abundant of which is 3-(2-deoxy-β-D-erythro-pentofuranosyl)pyrimido[1,2-α]purin-10(3H)-one (M1dG). Increased levels of M1dG are positively correlated with a variety of physiological and pathophysiological conditions including aging, bacterial infection, and cancer. Recently, our laboratory reported the oxidative metabolism of M1dG to 6-oxo-M1dG in nuclear DNA isolated from multiple human and rodent cell lines. Heat-denaturation or proteinase K-treatment of nuclear lysates ablated conversion of M1dG to 6-oxo-M1dG in double-stranded oligonucleotides, suggesting that the oxidation is enzymatic. Preincubation of nuclear lysates with iron or alpha-ketoglutarate increased 6-oxo-M1dG formation, and nuclear lysates preincubated with either N-oxalylglycine or IOX1, two structurally distinct broad-spectrum alpha-ketoglutarate-dependent dioxygenase inhibitors, showed reduced 6-oxo-M1dG formation. Finally, treatment of RKO cells with JIB-04, a pan alpha-ketoglutarate-dependent Jumonji histone demethylase inhibitor, resulted in reduced levels of genomic 6-oxo-M1dG along with a concomitant increase in levels of M1dG. Collectively, these findings indicate the presence of a nuclear enzyme capable of converting M1dG to 6-oxo-M1dG in genomic DNA and support the hypothesis that the enzyme is a member of the alpha-ketoglutarate-dependent dioxygenase class of… Advisors/Committee Members: Ned A. Porter (committee member), Walter J. Chazin (committee member), Brian O. Bachmann (committee member), Lawrence J. Marnett (Committee Chair).

Subjects/Keywords: M1dG; chemical kinetics; endocannabinoids; cyclooxygenase-2; DNA adducts

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APA (6^th Edition):

Mitchener, M. M. (2017). A. Competition and allostery govern substrate selectivity of cyclooxygenase-2 B. Enzymatic oxidation of M1dG in the genome. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/15119

Chicago Manual of Style (16^th Edition):

Mitchener, Michelle Marie. “A. Competition and allostery govern substrate selectivity of cyclooxygenase-2 B. Enzymatic oxidation of M1dG in the genome.” 2017. Doctoral Dissertation, Vanderbilt University. Accessed January 17, 2021. http://hdl.handle.net/1803/15119.

MLA Handbook (7^th Edition):

Mitchener, Michelle Marie. “A. Competition and allostery govern substrate selectivity of cyclooxygenase-2 B. Enzymatic oxidation of M1dG in the genome.” 2017. Web. 17 Jan 2021.

Vancouver:

Mitchener MM. A. Competition and allostery govern substrate selectivity of cyclooxygenase-2 B. Enzymatic oxidation of M1dG in the genome. [Internet] [Doctoral dissertation]. Vanderbilt University; 2017. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1803/15119.

Council of Science Editors:

Mitchener MM. A. Competition and allostery govern substrate selectivity of cyclooxygenase-2 B. Enzymatic oxidation of M1dG in the genome. [Doctoral Dissertation]. Vanderbilt University; 2017. Available from: http://hdl.handle.net/1803/15119

17. Gujarathi, Jayesh R. Studies on metal complexes of parent and n4 substituted 5 chloro 2 hydroxyacetophenone thiosemicarbazone and their adducts; -.

Degree: Chemistry, 2013, North Maharashtra University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/36951

Abstract attached

Bibliography p.170-194

Advisors/Committee Members: Bendre, R S.

Subjects/Keywords: Heterocyclic base adducts; metal complexes

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APA (6^th Edition):

Gujarathi, J. R. (2013). Studies on metal complexes of parent and n4 substituted 5 chloro 2 hydroxyacetophenone thiosemicarbazone and their adducts; -. (Thesis). North Maharashtra University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/36951

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gujarathi, Jayesh R. “Studies on metal complexes of parent and n4 substituted 5 chloro 2 hydroxyacetophenone thiosemicarbazone and their adducts; -.” 2013. Thesis, North Maharashtra University. Accessed January 17, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/36951.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gujarathi, Jayesh R. “Studies on metal complexes of parent and n4 substituted 5 chloro 2 hydroxyacetophenone thiosemicarbazone and their adducts; -.” 2013. Web. 17 Jan 2021.

Vancouver:

Gujarathi JR. Studies on metal complexes of parent and n4 substituted 5 chloro 2 hydroxyacetophenone thiosemicarbazone and their adducts; -. [Internet] [Thesis]. North Maharashtra University; 2013. [cited 2021 Jan 17]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/36951.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gujarathi JR. Studies on metal complexes of parent and n4 substituted 5 chloro 2 hydroxyacetophenone thiosemicarbazone and their adducts; -. [Thesis]. North Maharashtra University; 2013. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/36951

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

18. Patel.Gauri. Dna adducts mutations and cancer; -.

Degree: life science, 2002, Gujarat University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/33460

none newline

-

Advisors/Committee Members: Agarwal,Y.K.

Subjects/Keywords: adducts; Dna; mutations and cancer

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APA (6^th Edition):

Patel.Gauri. (2002). Dna adducts mutations and cancer; -. (Thesis). Gujarat University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/33460

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Patel.Gauri. “Dna adducts mutations and cancer; -.” 2002. Thesis, Gujarat University. Accessed January 17, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/33460.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Patel.Gauri. “Dna adducts mutations and cancer; -.” 2002. Web. 17 Jan 2021.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

Patel.Gauri. Dna adducts mutations and cancer; -. [Internet] [Thesis]. Gujarat University; 2002. [cited 2021 Jan 17]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/33460.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Patel.Gauri. Dna adducts mutations and cancer; -. [Thesis]. Gujarat University; 2002. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/33460

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Rhodes University

19. Haigh, J M; Nassimbeni, L R; Pauptit, R A; Rodgers, A L. The structure of aliphatic amine adducts of uranyl acetylacetonate. I. Dioxobis(2,4-pentanedionato)mono (2-N-methylaminopentan-4-one)uranium(VI).

Degree: 1976, Rhodes University

URL: http://hdl.handle.net/10962/d1006077

► Crystals of the title compound are monoclinic with a= 8.314 (5), b= 22.723 (9), c= 12.589 (6) A, /3= 123.0 (2t, Z=4, space group P2dc.… (more)

Subjects/Keywords: Aliphatic amine adducts; Uranyl acetylacetonate

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APA (6^th Edition):

Haigh, J M; Nassimbeni, L R; Pauptit, R A; Rodgers, A. L. (1976). The structure of aliphatic amine adducts of uranyl acetylacetonate. I. Dioxobis(2,4-pentanedionato)mono (2-N-methylaminopentan-4-one)uranium(VI). (Thesis). Rhodes University. Retrieved from http://hdl.handle.net/10962/d1006077

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Haigh, J M; Nassimbeni, L R; Pauptit, R A; Rodgers, A L. “The structure of aliphatic amine adducts of uranyl acetylacetonate. I. Dioxobis(2,4-pentanedionato)mono (2-N-methylaminopentan-4-one)uranium(VI).” 1976. Thesis, Rhodes University. Accessed January 17, 2021. http://hdl.handle.net/10962/d1006077.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Haigh, J M; Nassimbeni, L R; Pauptit, R A; Rodgers, A L. “The structure of aliphatic amine adducts of uranyl acetylacetonate. I. Dioxobis(2,4-pentanedionato)mono (2-N-methylaminopentan-4-one)uranium(VI).” 1976. Web. 17 Jan 2021.

Vancouver:

Haigh, J M; Nassimbeni, L R; Pauptit, R A; Rodgers AL. The structure of aliphatic amine adducts of uranyl acetylacetonate. I. Dioxobis(2,4-pentanedionato)mono (2-N-methylaminopentan-4-one)uranium(VI). [Internet] [Thesis]. Rhodes University; 1976. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/10962/d1006077.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Haigh, J M; Nassimbeni, L R; Pauptit, R A; Rodgers AL. The structure of aliphatic amine adducts of uranyl acetylacetonate. I. Dioxobis(2,4-pentanedionato)mono (2-N-methylaminopentan-4-one)uranium(VI). [Thesis]. Rhodes University; 1976. Available from: http://hdl.handle.net/10962/d1006077

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

20. Liu, Ming. Formation of borane adducts and some complexes starting from N-heterocyclic carbenes.

Degree: 2017, Technische Universität Dortmund

URL: https://doi.org/10.21268/20170406-092952 ; https://nbn-resolving.org/urn:nbn:de:gbv:104-20170406-093241-2 ; https://dokumente.ub.tu-clausthal.de/receive/clausthal_mods_00000494 ; https://dokumente.ub.tu-clausthal.de/servlets/MCRFileNodeServlet/clausthal_derivate_00000268/Db113054.pdf

► The conjugated mesomeric betaines 2-(1-phenyl-4H-1,2,4-triazolium-4-yl)phenolates and 2-(imidazolium-1-yl)phenolates are in equilibrium with the corresponding N-heterocyclic carbenes (NHCs) which can be trapped as thiones (X-ray analysis). Both… (more)

Subjects/Keywords: Abschlussarbeit; doctoral thesis; ddc:540; N-Heterocyclic carbenes – Borane-Adducts – complexes

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APA (6^th Edition):

Liu, M. (2017). Formation of borane adducts and some complexes starting from N-heterocyclic carbenes. (Thesis). Technische Universität Dortmund. Retrieved from https://doi.org/10.21268/20170406-092952 ; https://nbn-resolving.org/urn:nbn:de:gbv:104-20170406-093241-2 ; https://dokumente.ub.tu-clausthal.de/receive/clausthal_mods_00000494 ; https://dokumente.ub.tu-clausthal.de/servlets/MCRFileNodeServlet/clausthal_derivate_00000268/Db113054.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Liu, Ming. “Formation of borane adducts and some complexes starting from N-heterocyclic carbenes.” 2017. Thesis, Technische Universität Dortmund. Accessed January 17, 2021. https://doi.org/10.21268/20170406-092952 ; https://nbn-resolving.org/urn:nbn:de:gbv:104-20170406-093241-2 ; https://dokumente.ub.tu-clausthal.de/receive/clausthal_mods_00000494 ; https://dokumente.ub.tu-clausthal.de/servlets/MCRFileNodeServlet/clausthal_derivate_00000268/Db113054.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Liu, Ming. “Formation of borane adducts and some complexes starting from N-heterocyclic carbenes.” 2017. Web. 17 Jan 2021.

Vancouver:

Liu M. Formation of borane adducts and some complexes starting from N-heterocyclic carbenes. [Internet] [Thesis]. Technische Universität Dortmund; 2017. [cited 2021 Jan 17]. Available from: https://doi.org/10.21268/20170406-092952 ; https://nbn-resolving.org/urn:nbn:de:gbv:104-20170406-093241-2 ; https://dokumente.ub.tu-clausthal.de/receive/clausthal_mods_00000494 ; https://dokumente.ub.tu-clausthal.de/servlets/MCRFileNodeServlet/clausthal_derivate_00000268/Db113054.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Liu M. Formation of borane adducts and some complexes starting from N-heterocyclic carbenes. [Thesis]. Technische Universität Dortmund; 2017. Available from: https://doi.org/10.21268/20170406-092952 ; https://nbn-resolving.org/urn:nbn:de:gbv:104-20170406-093241-2 ; https://dokumente.ub.tu-clausthal.de/receive/clausthal_mods_00000494 ; https://dokumente.ub.tu-clausthal.de/servlets/MCRFileNodeServlet/clausthal_derivate_00000268/Db113054.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Iowa

21. Li, Miao. Protein adducts and crosslinking by reactive metabolites of polychlorinated biphenyls (PCBs).

Degree: PhD, Human Toxicology, 2015, University of Iowa

URL: https://ir.uiowa.edu/etd/1984

► Polychlorinated biphenyls (PCBs) are the persistent environmental pollutants with the continuous concerns over adverse human health effects. As semi-volatile compounds, PCBs were found in… (more)

▼ Polychlorinated biphenyls (PCBs) are the persistent environmental pollutants with the continuous concerns over adverse human health effects. As semi-volatile compounds, PCBs were found in indoor and outdoor air. The observation of high levels of airborne PCBs in old school buildings raised the concerns of inhalation exposure and toxicity of PCBs. Lower chlorinated PCBs (LC-PCBs), major components of airborne PCBs, are subject to biotranformation. In vitro and in vivo studies revealed that reactive metabolites of LC-PCBs formed covalent adducts on DNA and proteins. The hypothesis of the project is that the reactive metabolites of LC-PCBs are able to form adducts on proteins or even protein crosslinks, and the formation of protein adducts and crosslinks causes the dysfunction of the target proteins. In addition, the objectives of the project are also to identify protein targets by PCB metabolites, which may be related to the mechanism of toxicity of LC-PCBs. The alkaline permethylation (AP) was established and optimized to identify and measure the protein adducts from LC-PCB metabolites. The AP method evidenced PCB metabolites formed protein adducts through the sulfhydryl groups and also one molecule of PCB quinoid metabolites was able to bind to more than one protein. Application of cytochrome c as the model protein revealed PCB quinoid metabolites also formed adducts on lysine and glutamic acid. The adduct formation and crosslinks caused the dysfunction of cytochrome c. In addition, the quinone protein adducts still kept the ability for redox reactions, which may lead to unexpected toxicity. The SILAC method was applied to identify the target proteins in the samples of in vitro proteome incubation. The instability of PCB quinone protein adducts was found by further reaction of quinone protein adducts. This may be the reason why cysteine-PCB quinone adducts were not frequently identified by proteomics method. The further understanding of protein adducts by reactive PCB metabolites helps to identify the target proteins, and ultimately reveal the role of protein adducts impacting on human health. Advisors/Committee Members: Ludewig, Gabriele (supervisor).

Subjects/Keywords: publicabstract; Polychlorinated Biphynels; Protein Adducts; Proteomics; Quinone; Reactive Metabolites; SILAC; Toxicology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Li, M. (2015). Protein adducts and crosslinking by reactive metabolites of polychlorinated biphenyls (PCBs). (Doctoral Dissertation). University of Iowa. Retrieved from https://ir.uiowa.edu/etd/1984

Chicago Manual of Style (16^th Edition):

Li, Miao. “Protein adducts and crosslinking by reactive metabolites of polychlorinated biphenyls (PCBs).” 2015. Doctoral Dissertation, University of Iowa. Accessed January 17, 2021. https://ir.uiowa.edu/etd/1984.

MLA Handbook (7^th Edition):

Li, Miao. “Protein adducts and crosslinking by reactive metabolites of polychlorinated biphenyls (PCBs).” 2015. Web. 17 Jan 2021.

Vancouver:

Li M. Protein adducts and crosslinking by reactive metabolites of polychlorinated biphenyls (PCBs). [Internet] [Doctoral dissertation]. University of Iowa; 2015. [cited 2021 Jan 17]. Available from: https://ir.uiowa.edu/etd/1984.

Council of Science Editors:

Li M. Protein adducts and crosslinking by reactive metabolites of polychlorinated biphenyls (PCBs). [Doctoral Dissertation]. University of Iowa; 2015. Available from: https://ir.uiowa.edu/etd/1984

Hong Kong University of Science and Technology

22. Leung, Ming Kit CHEM. Development and application of liquid chromatography tandem mass spectrometric method for detecting DNA/RNA adducts as biomarkers of carcinogen exposure : a case study with aristolochic acids.

Degree: 2016, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-88916 ; https://doi.org/10.14711/thesis-b1585430 ; http://repository.ust.hk/ir/bitstream/1783.1-88916/1/th_redirect.html

► Biomarker is an important indicator that measures the events occurring in a biological system. Apart from being used as a sign to disclose the health… (more)

▼ Biomarker is an important indicator that measures the events occurring in a biological system. Apart from being used as a sign to disclose the health status of an individual, biomarker is extremely useful in both toxicological research as well as biological monitoring. In addition to the occurrence of natural disease, exposure to toxic or specific environments may lead to fatal alteration of the biological system of a subject. However, if such alteration can be detected in the early stage, life will be spared upon appropriate medication. As a consequence, the evaluation of biomarker is extremely useful in the prevention of mortality. Substances which cause renal damage are defined as nephrotoxic. Aristolochic acid (AA) is one of the examples of nephrotoxic substances. Research studies and literature reveal that AA is strongly associated with the cases of renal fibrosis reported during the late 80s. Evidence has shown that AA will covalently bind to DNA to form stable adducts with remarkable persistence which can be detected after a reasonable long period of time. However, conventional analyses of DNA-AA adducts involve both tedious and invasive DNA isolation procedures in target organ. Herein, we report a non-invasive approach to detect and quantify DNA-AA adducts in rat urine rather than in tissue samples. The developed method entails extraction and enrichment of DNA-AA adducts in urine samples by solid phase extraction, followed by detection with the assistance of liquid chromatography-tandem mass spectrometry (LC-MS/MS). Since the newly developed method is to replace the old and traditional invasive method for adducts detection, method comparison is performed so as to prove that the developed method is of advantageous. Apart from DNA, emerging evidence showed that RNA will be attacked under the influence of carcinogens. The impact is similar to that of DNA where the formation of covalent adducts may be resulted. However, since RNA does not possess proofreading enzyme such as DNA polymerase, there is no repair system for the elimination of the covalent adducts. Instead, RNA relies on enzymatic degradation or decay. Hence, RNA adducts may be released as free ribonucleosides and excreted through urinary system. As a result, we use an approach which is similar to DNA to detect the RNA-AA adducts in urine samples by UPLC-MS/MS. On the other hand, since the synthesis of proteins heavily relies on the integrity of RNA, we have a hypothesis that the deviation in RNA sequence will ultimately lead to mis-folding or truncation of proteins, therefore resulting in AA associated diseases such as kidney fibrosis. Comparing to the traditional detection methods, the advantages of our developed method by utilizing mass spectrometry detection include high sensitivity and good selectivity. Identities of adducts have been made more promising and quantities of adducts are accurately evaluated. Although the traditional methods are interchangeable with our newly developed method, the enzymatic treatment of the old methods is still vital…

Subjects/Keywords: Liquid chromatography ; Tandem mass spectrometry ; Biochemical markers ; DNA adducts ; Analysis ; RNA

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APA (6^th Edition):

Leung, M. K. C. (2016). Development and application of liquid chromatography tandem mass spectrometric method for detecting DNA/RNA adducts as biomarkers of carcinogen exposure : a case study with aristolochic acids. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-88916 ; https://doi.org/10.14711/thesis-b1585430 ; http://repository.ust.hk/ir/bitstream/1783.1-88916/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Leung, Ming Kit CHEM. “Development and application of liquid chromatography tandem mass spectrometric method for detecting DNA/RNA adducts as biomarkers of carcinogen exposure : a case study with aristolochic acids.” 2016. Thesis, Hong Kong University of Science and Technology. Accessed January 17, 2021. http://repository.ust.hk/ir/Record/1783.1-88916 ; https://doi.org/10.14711/thesis-b1585430 ; http://repository.ust.hk/ir/bitstream/1783.1-88916/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Leung, Ming Kit CHEM. “Development and application of liquid chromatography tandem mass spectrometric method for detecting DNA/RNA adducts as biomarkers of carcinogen exposure : a case study with aristolochic acids.” 2016. Web. 17 Jan 2021.

Vancouver:

Leung MKC. Development and application of liquid chromatography tandem mass spectrometric method for detecting DNA/RNA adducts as biomarkers of carcinogen exposure : a case study with aristolochic acids. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2016. [cited 2021 Jan 17]. Available from: http://repository.ust.hk/ir/Record/1783.1-88916 ; https://doi.org/10.14711/thesis-b1585430 ; http://repository.ust.hk/ir/bitstream/1783.1-88916/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Leung MKC. Development and application of liquid chromatography tandem mass spectrometric method for detecting DNA/RNA adducts as biomarkers of carcinogen exposure : a case study with aristolochic acids. [Thesis]. Hong Kong University of Science and Technology; 2016. Available from: http://repository.ust.hk/ir/Record/1783.1-88916 ; https://doi.org/10.14711/thesis-b1585430 ; http://repository.ust.hk/ir/bitstream/1783.1-88916/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Hong Kong University of Science and Technology

23. Wang, Yinan CHEM. Development and application of liquid chromatography and tandem mass spectrometry methods to quantitate antibacterial nitrofurans and associated DNA and RNA adducts.

Degree: 2018, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-97209 ; https://doi.org/10.14711/thesis-991012587065103412 ; http://repository.ust.hk/ir/bitstream/1783.1-97209/1/th_redirect.html

► Nitrofuran (NF) is a class of broad-spectrum veterinary drugs for the treatment of microbial infections. They are highly effective in suppressing and killing most kinds… (more)

Subjects/Keywords: DNA adducts ; Nitrofurans ; Liquid chromatography ; Tandem mass spectrometry

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APA (6^th Edition):

Wang, Y. C. (2018). Development and application of liquid chromatography and tandem mass spectrometry methods to quantitate antibacterial nitrofurans and associated DNA and RNA adducts. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-97209 ; https://doi.org/10.14711/thesis-991012587065103412 ; http://repository.ust.hk/ir/bitstream/1783.1-97209/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wang, Yinan CHEM. “Development and application of liquid chromatography and tandem mass spectrometry methods to quantitate antibacterial nitrofurans and associated DNA and RNA adducts.” 2018. Thesis, Hong Kong University of Science and Technology. Accessed January 17, 2021. http://repository.ust.hk/ir/Record/1783.1-97209 ; https://doi.org/10.14711/thesis-991012587065103412 ; http://repository.ust.hk/ir/bitstream/1783.1-97209/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wang, Yinan CHEM. “Development and application of liquid chromatography and tandem mass spectrometry methods to quantitate antibacterial nitrofurans and associated DNA and RNA adducts.” 2018. Web. 17 Jan 2021.

Vancouver:

Wang YC. Development and application of liquid chromatography and tandem mass spectrometry methods to quantitate antibacterial nitrofurans and associated DNA and RNA adducts. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2018. [cited 2021 Jan 17]. Available from: http://repository.ust.hk/ir/Record/1783.1-97209 ; https://doi.org/10.14711/thesis-991012587065103412 ; http://repository.ust.hk/ir/bitstream/1783.1-97209/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wang YC. Development and application of liquid chromatography and tandem mass spectrometry methods to quantitate antibacterial nitrofurans and associated DNA and RNA adducts. [Thesis]. Hong Kong University of Science and Technology; 2018. Available from: http://repository.ust.hk/ir/Record/1783.1-97209 ; https://doi.org/10.14711/thesis-991012587065103412 ; http://repository.ust.hk/ir/bitstream/1783.1-97209/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Minnesota

24. Sangaraju, Dewakar. Mass Spectrometry Based Quantification of 1, 3-Butadiene Induced DNA Adducts: Potential Biomarkers of Cancer Risk.

Degree: PhD, Medicinal Chemistry, 2014, University of Minnesota

URL: http://hdl.handle.net/11299/177098

► Chemical carcinogenesis involves metabolic activation of carcinogens to electrophilic species which can react with important cellular biomolecules including DNA to form covalent adducts. Covalent carcinogen-DNA… (more)

▼ Chemical carcinogenesis involves metabolic activation of carcinogens to electrophilic species which can react with important cellular biomolecules including DNA to form covalent adducts. Covalent carcinogen-DNA adducts which are not removed by DNA repair mechanisms can induce transforming mutations, ultimately leading to cancer. Hence, carcinogen-DNA adducts are deemed the ultimate biomarkers of carcinogen exposure, metabolic activation, and possibly of cancer risk. 1,3-Butadiene (BD) is a recognized human and animal carcinogen present in cigarette smoke, automobile exhaust, wood fires, and also in some occupational settings such as BD monomer and polymer plants. BD is metabolically activated by CYP2E1 to form three electrophilic epoxides: 3,4-epoxy-1-butene (EB), 3,4-epoxy-1,2-butanediol (EBD), and 1,2,3,4-diepoxybutane (DEB). EB, EBD, and DEB can modify DNA bases to form covalent DNA adducts such as N-7-(1-hydroxy-3-buten-2-yl) guanine (EB-GII), N7-(2, 3, 4-trihydroxybut-1-yl)-guanine (N7-THBG) and 1,4-bis-(guan-7-yl)-2,3-butanediol (bis-N7G-BD). Although BD-DNA adducts had been successfully detected and quantified in tissues of laboratory animals exposed to relatively high concentrations of BD ( ≥ 6.25 ppm), they had not been previously quantified in humans, preventing their use as biomarkers of BD exposure, metabolic activation, and cancer risk. The main purpose of this research was to develop ultra-sensitive bioanalytical methodologies based on mass spectrometry to enable the detection and quantitation of BD-DNA adducts in animals treated with sup-ppm levels of BD and in exposed human populations. In Chapter 2 of the thesis, a novel nanoHPLC-nanoESI+-MS/MS method was developed for sensitive, accurate, and precise quantitation of BD-induced guanine-guanine cross-links (1,4-bis-(guan-7-yl)-2,3,-butanediol, bis-N7G-BD) in tissues of laboratory mice treated with low - sub-ppm concentrations of BD (0.5-1.5 ppm) which approximate human occupational exposure to BD (1 ppm). Bis-N7G-BD concentrations increased in a concentration-dependent manner in mouse liver DNA as a function of BD exposure. In Chapter 3 of this Thesis, we investigated DNA repair mechanisms responsible for bis-N7G-BD repair using isogenic Chinese hamster lung fibroblasts proficient or deficient in nucleotide excision repair (NER) and Fanconi Anemia (FA) repair pathways. We found that while both pathways contributed to bis-N7G-BD removal, FA pathway was most effective at alleviating the toxicity and replication blockage imposed by bis-N7G-BD cross-links. To enable BD-DNA adduct detection in humans, we developed an isotope dilution capillary HPLC-ESI+-HRMS/MS methodology for the most abundant BD-DNA adducts identified in vivo: N7-(2,3,4-trihydroxybut-1-yl)-guanine (N7-THBG) (Chapter 4). This method was successfully applied to quantify N7-THBG adducts in blood leukocyte DNA of smokers, nonsmokers, and occupationally exposed workers. In addition, we have developed an isotope dilution nanoLC/ESI+-HRMS3 methodology for the quantitation of BD-induced…

Subjects/Keywords: 1; 3-Butadiene; Biomarkers; DNA adducts; humans; Mass spectrometry

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APA (6^th Edition):

Sangaraju, D. (2014). Mass Spectrometry Based Quantification of 1, 3-Butadiene Induced DNA Adducts: Potential Biomarkers of Cancer Risk. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/177098

Chicago Manual of Style (16^th Edition):

Sangaraju, Dewakar. “Mass Spectrometry Based Quantification of 1, 3-Butadiene Induced DNA Adducts: Potential Biomarkers of Cancer Risk.” 2014. Doctoral Dissertation, University of Minnesota. Accessed January 17, 2021. http://hdl.handle.net/11299/177098.

MLA Handbook (7^th Edition):

Sangaraju, Dewakar. “Mass Spectrometry Based Quantification of 1, 3-Butadiene Induced DNA Adducts: Potential Biomarkers of Cancer Risk.” 2014. Web. 17 Jan 2021.

Vancouver:

Sangaraju D. Mass Spectrometry Based Quantification of 1, 3-Butadiene Induced DNA Adducts: Potential Biomarkers of Cancer Risk. [Internet] [Doctoral dissertation]. University of Minnesota; 2014. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/11299/177098.

Council of Science Editors:

Sangaraju D. Mass Spectrometry Based Quantification of 1, 3-Butadiene Induced DNA Adducts: Potential Biomarkers of Cancer Risk. [Doctoral Dissertation]. University of Minnesota; 2014. Available from: http://hdl.handle.net/11299/177098

Latrobe University

25. Forrest, Robert. DNA Damage Responses Elicited by Anthracycline-DNA Adducts.

Degree: PhD, 2010, Latrobe University

URL: http://hdl.handle.net/1959.9/511365

►

Thesis (Ph.D.) - La Trobe University, 2010

Submission note: “A Thesis Submitted In Total Fulfilment of the Requirements for the Degree of Doctor of Philosophy… (more)

Subjects/Keywords: DNA adducts.; DNA damage.

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APA (6^th Edition):

Forrest, R. (2010). DNA Damage Responses Elicited by Anthracycline-DNA Adducts. (Doctoral Dissertation). Latrobe University. Retrieved from http://hdl.handle.net/1959.9/511365

Chicago Manual of Style (16^th Edition):

Forrest, Robert. “DNA Damage Responses Elicited by Anthracycline-DNA Adducts.” 2010. Doctoral Dissertation, Latrobe University. Accessed January 17, 2021. http://hdl.handle.net/1959.9/511365.

MLA Handbook (7^th Edition):

Forrest, Robert. “DNA Damage Responses Elicited by Anthracycline-DNA Adducts.” 2010. Web. 17 Jan 2021.

Vancouver:

Forrest R. DNA Damage Responses Elicited by Anthracycline-DNA Adducts. [Internet] [Doctoral dissertation]. Latrobe University; 2010. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1959.9/511365.

Council of Science Editors:

Forrest R. DNA Damage Responses Elicited by Anthracycline-DNA Adducts. [Doctoral Dissertation]. Latrobe University; 2010. Available from: http://hdl.handle.net/1959.9/511365

University of North Texas

26. McDougald, Roy N., Jr. Photochemical and Photophysical Properties of Mononuclear and Multinuclear Closed Shell D10 Coinage Metal Complexes and Their Metallo-organometallic Adducts.

Degree: 2013, University of North Texas

URL: https://digital.library.unt.edu/ark:/67531/metadc407759/

► This dissertation covers the studies of two major topics: the photochemistry of mononuclear and multinuclear gold(I) complexes and synthetic approaches to tailor photophysical properties of… (more)

Subjects/Keywords: Organometallic; trinuclear gold complex; π-acid/π-base adducts; aurophilicity; heterobimetallic

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APA (6^th Edition):

McDougald, Roy N., J. (2013). Photochemical and Photophysical Properties of Mononuclear and Multinuclear Closed Shell D10 Coinage Metal Complexes and Their Metallo-organometallic Adducts. (Thesis). University of North Texas. Retrieved from https://digital.library.unt.edu/ark:/67531/metadc407759/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

McDougald, Roy N., Jr. “Photochemical and Photophysical Properties of Mononuclear and Multinuclear Closed Shell D10 Coinage Metal Complexes and Their Metallo-organometallic Adducts.” 2013. Thesis, University of North Texas. Accessed January 17, 2021. https://digital.library.unt.edu/ark:/67531/metadc407759/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

McDougald, Roy N., Jr. “Photochemical and Photophysical Properties of Mononuclear and Multinuclear Closed Shell D10 Coinage Metal Complexes and Their Metallo-organometallic Adducts.” 2013. Web. 17 Jan 2021.

Vancouver:

McDougald, Roy N. J. Photochemical and Photophysical Properties of Mononuclear and Multinuclear Closed Shell D10 Coinage Metal Complexes and Their Metallo-organometallic Adducts. [Internet] [Thesis]. University of North Texas; 2013. [cited 2021 Jan 17]. Available from: https://digital.library.unt.edu/ark:/67531/metadc407759/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

McDougald, Roy N. J. Photochemical and Photophysical Properties of Mononuclear and Multinuclear Closed Shell D10 Coinage Metal Complexes and Their Metallo-organometallic Adducts. [Thesis]. University of North Texas; 2013. Available from: https://digital.library.unt.edu/ark:/67531/metadc407759/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

27. Hong, Li. Syntheses and Immunological Detection of Oxidized Lipid-Derived Protein and Phosphatidylethanolamine Modifications.

Degree: PhD, Chemistry, 2011, Case Western Reserve University School of Graduate Studies

URL: http://rave.ohiolink.edu/etdc/view?acc_num=case1296331868

► Phospholipid oxidation and its products are increasingly believed to be associated with diseases. Oxidative cleavage of phospholipids containing docosahexaenoic acid produces reactive electrophilic 4-hydroxy-7-oxohept-5-enoates… (more)

▼ Phospholipid oxidation and its products are increasingly believed to be associated with diseases. Oxidative cleavage of phospholipids containing docosahexaenoic acid produces reactive electrophilic 4-hydroxy-7-oxohept-5-enoates and 4-keto-7-oxohept-5-enoates. The former one can convert the primary amino group of protein lysyl residues into 2-(ω-carboxyethyl)pyrrole (CEP) derivatives, while the latter one can generate 4-oxo-heptanedioic amide (OHdiA) derivatives. Arachidonyl acid containing phospholipids and Linoleic acid containing phospholipids undergo the same transformations to generate2-(ω-carboxypropyl)pyrrole derivatives (CPP), 2-(ω-carboxyheptyl)pyrrole (CHP) and 2-pentylpyrrole derivatives (PP). For the first time, OHdiA derivatives of proteins, a dipeptide, and an ethanolamine phospholipid was synthesized, characterized and found to be angiogenic. Anti-OHdiA polyclonal antibodies are generated and purified. ELISA experiments confirm that those antibodies are specific to the OHdiA epitopes with little cross-reaction from CEPs, CPPs, and CHPs. CEPs and OHdiAs are generated during inflammation and wound healing and accumulate at high levels in aging tissues. The CEP-driven and OHdiA-driven angiogenesis may be different from other proangiogenic growth factors, including VEGF, providing an attractive new therapeutic target, especially in cancers resistant to anti-VEGF therapy and as an adjunct to anti-VEGF therapy for AMD. To further investigate the influence of carboxyalkyl chain length in carboxyalkyl pyrrole compounds, three different compounds other than CEP, CPP and CHP were also synthesized and characterized. A new and effective synthesis of CAP is described which facilitates scale up because intermediates are crystalline. This improved synthesis facilitates their use as agonists to test their receptor-mediated responses, and as standards to measure levels of them in vivo. The CEP levels of both Melanoma patients and healthy controls were measured by the ELISA assay using CEP-HSA as standard. It was found that there was no significant difference between the two groups. Similarly, five melanoma tumor-injected WT-WT mice, five melanoma tumor-injected WT-TLR2 mice, seven melanoma tumor-injected TLR2-WT mice, three CEP mAb-injected plus wound assay mice and two nonspecific IgG2a-injected plus wound assay mice were measured for their CEP protein adducts levels by competitive ELISA, and no significant difference was found in CEP levels among different mice. Advisors/Committee Members: Salomon, Robet (Advisor), Zagorski, Michael (Committee Chair).

Subjects/Keywords: Chemistry; Lipid-derived proteins; phospholipids; CEP; adducts; CDCl3; NMR

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hong, L. (2011). Syntheses and Immunological Detection of Oxidized Lipid-Derived Protein and Phosphatidylethanolamine Modifications. (Doctoral Dissertation). Case Western Reserve University School of Graduate Studies. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=case1296331868

Chicago Manual of Style (16^th Edition):

Hong, Li. “Syntheses and Immunological Detection of Oxidized Lipid-Derived Protein and Phosphatidylethanolamine Modifications.” 2011. Doctoral Dissertation, Case Western Reserve University School of Graduate Studies. Accessed January 17, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=case1296331868.

MLA Handbook (7^th Edition):

Hong, Li. “Syntheses and Immunological Detection of Oxidized Lipid-Derived Protein and Phosphatidylethanolamine Modifications.” 2011. Web. 17 Jan 2021.

Vancouver:

Hong L. Syntheses and Immunological Detection of Oxidized Lipid-Derived Protein and Phosphatidylethanolamine Modifications. [Internet] [Doctoral dissertation]. Case Western Reserve University School of Graduate Studies; 2011. [cited 2021 Jan 17]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=case1296331868.

Council of Science Editors:

Hong L. Syntheses and Immunological Detection of Oxidized Lipid-Derived Protein and Phosphatidylethanolamine Modifications. [Doctoral Dissertation]. Case Western Reserve University School of Graduate Studies; 2011. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=case1296331868

University of Cincinnati

28. VIETAS, JAY A. CO-EXPOSURE OF ARSENITE AND BENZO(a)PYRENE: EFFECT OF GLUTATHIONE ON DNA ADDUCT LEVELS.

Degree: PhD, Medicine : Epidemiology (Environmental Health), 2005, University of Cincinnati

URL: http://rave.ohiolink.edu/etdc/view?acc_num=ucin1132172369

► Humans are considered the most sensitive species to arsenic exposure with increased risk to skin, lung and bladder cancer. Epidemiologic studies of workers simultaneously exposed… (more)

▼ Humans are considered the most sensitive species to arsenic exposure with increased risk to skin, lung and bladder cancer. Epidemiologic studies of workers simultaneously exposed to benzo[a]pyrene (BaP) and arsenite (As) report additive to multiplicative effects. These studies are supported by both in vitro and in animal studies demonstrating an increase in BaP DNA adduct levels when co-treated with BaP and arsenite than when treated with BaP alone. Glutathione, the major thiol compound responsible for maintaining redox homeostasis, may provide cellular protection against arsenite’s ability to increase the likelihood of DNA damage. We examined the effect of arsenite and glutathione on BaP DNA adduct levels in the absence of cellular processes, characterized the effect of modulating glutathione levels in Hepa-1 cells and in C57BL/6 mice through the use of buthionine sulfoximine (BSO) and glutathione ethyl ester (GSHEE) treatment, and examined the impact of arsenite and BaP coexposure on glutathione deficient knockout mice and mouse embryonic fibroblasts. In the absence of glutathione S-transferases, normal intracellular glutathione concentrations did not reduce BaP-DNA adduct levels and arsenite increased adduct levels (p=0.0028) via an unidentified mechanism. A 24 hour pretreatment of arsenite in Hepa-1 cells potentiated BaP DNA adduct formation (p=0.002), although lower than observed with a 30 minute pretreatment period. Glutathione modulation, through pharmacological methods or by examining glutathione deficient models, demonstrated no effect on DNA adduct levels after BaP treatment, or after arsenite BaP co-treatment. Experimentation with C57/BL6 mice showed that arsenic co-treatment increased average BaP adduct levels in both lung and skin; the increase was statistically significant in the lung (p=0.048). A reduction in glutathione level increased BaP adduct levels, although only significantly in the skin of mice treated with BSO (p=0.028). Treatment with GSHEE did not reduce adduct levels in any tissue measured. These results are consistent with previous in vitro and in vivo findings and suggest that glutathione plays a minor role in arsenic’s ability to potentiate BaP DNA adduct formation. Advisors/Committee Members: Talaska, Dr. Glenn (Advisor).

Subjects/Keywords: arsenic; glutathione; cancer; DNA adducts

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APA (6^th Edition):

VIETAS, J. A. (2005). CO-EXPOSURE OF ARSENITE AND BENZO(a)PYRENE: EFFECT OF GLUTATHIONE ON DNA ADDUCT LEVELS. (Doctoral Dissertation). University of Cincinnati. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=ucin1132172369

Chicago Manual of Style (16^th Edition):

VIETAS, JAY A. “CO-EXPOSURE OF ARSENITE AND BENZO(a)PYRENE: EFFECT OF GLUTATHIONE ON DNA ADDUCT LEVELS.” 2005. Doctoral Dissertation, University of Cincinnati. Accessed January 17, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1132172369.

MLA Handbook (7^th Edition):

VIETAS, JAY A. “CO-EXPOSURE OF ARSENITE AND BENZO(a)PYRENE: EFFECT OF GLUTATHIONE ON DNA ADDUCT LEVELS.” 2005. Web. 17 Jan 2021.

Vancouver:

VIETAS JA. CO-EXPOSURE OF ARSENITE AND BENZO(a)PYRENE: EFFECT OF GLUTATHIONE ON DNA ADDUCT LEVELS. [Internet] [Doctoral dissertation]. University of Cincinnati; 2005. [cited 2021 Jan 17]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ucin1132172369.

Council of Science Editors:

VIETAS JA. CO-EXPOSURE OF ARSENITE AND BENZO(a)PYRENE: EFFECT OF GLUTATHIONE ON DNA ADDUCT LEVELS. [Doctoral Dissertation]. University of Cincinnati; 2005. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ucin1132172369

29. NC DOCKS at The University of North Carolina at Greensboro; Proctor, Nathanael K. Studying the effects of DNA methylation on adduct formation using molecular modeling.

Degree: 2011, NC Docks

URL: http://libres.uncg.edu/ir/uncg/f/Proctor_uncg_0154M_10650.pdf

► The goal of this study was to use molecular modeling to compare and analyze the molecular structure of a double-stranded DNA fragment, and the effects… (more)

Subjects/Keywords: Adducts, DNA; DNA $x Structure; DNA $x Methylation

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APA (6^th Edition):

NC DOCKS at The University of North Carolina at Greensboro; Proctor, N. K. (2011). Studying the effects of DNA methylation on adduct formation using molecular modeling. (Thesis). NC Docks. Retrieved from http://libres.uncg.edu/ir/uncg/f/Proctor_uncg_0154M_10650.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

NC DOCKS at The University of North Carolina at Greensboro; Proctor, Nathanael K. “Studying the effects of DNA methylation on adduct formation using molecular modeling.” 2011. Thesis, NC Docks. Accessed January 17, 2021. http://libres.uncg.edu/ir/uncg/f/Proctor_uncg_0154M_10650.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

NC DOCKS at The University of North Carolina at Greensboro; Proctor, Nathanael K. “Studying the effects of DNA methylation on adduct formation using molecular modeling.” 2011. Web. 17 Jan 2021.

Vancouver:

NC DOCKS at The University of North Carolina at Greensboro; Proctor NK. Studying the effects of DNA methylation on adduct formation using molecular modeling. [Internet] [Thesis]. NC Docks; 2011. [cited 2021 Jan 17]. Available from: http://libres.uncg.edu/ir/uncg/f/Proctor_uncg_0154M_10650.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

NC DOCKS at The University of North Carolina at Greensboro; Proctor NK. Studying the effects of DNA methylation on adduct formation using molecular modeling. [Thesis]. NC Docks; 2011. Available from: http://libres.uncg.edu/ir/uncg/f/Proctor_uncg_0154M_10650.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

30. Freitas, Florêncio Porto. Investigação de potenciais biomarcadores redox - um enfoque em aldeídos e seus produtos.

Degree: PhD, Bioquímica, 2014, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/46/46131/tde-30092014-093431/ ;

► As espécies reativas são associadas a processos toxicológicos e fisiopatológicos, agindo como importantes mediadores, por exemplo, na sinalização celular. Diversas classes de compostos têm sido… (more)

▼ As espécies reativas são associadas a processos toxicológicos e fisiopatológicos, agindo como importantes mediadores, por exemplo, na sinalização celular. Diversas classes de compostos têm sido utilizadas como possíveis biomarcadores de estresse redox, destacando-se os aldeídos α,β-insaturados, capazes de alquilar biomoléculas como o DNA. Para evitar efeitos deletérios, estes aldeídos são detoxificados por glutationilação e posterior metabolização a derivados mercaptúricos. Contudo, avaliar o estado redox em sistemas biológicos ainda é tarefa bastante complexa, sendo a dificuldade em quantificar de forma prática e acurada os efeitos de sinalização e/ou dano molecular o maior problema dos estudos redox. Assim, o objetivo deste trabalho foi desenvolver métodos acurados e sensíveis de análise de potenciais biomarcadores de estresse redox, isto é: nucleosídeos modificados, aldeídos endógenos e exógenos, glutationa e produtos de glutationilação, e avaliá-los em sistemas modelos, celular e animal, e em humanos. A avaliação dos níveis urinários de três nucleosídeos modificados por metodologia de HPLC-MS/MS desenvolvida pelo grupo em moradores da cidade de São Paulo - região com poluição atmosférica - demonstrou aumento significativo de 1,N2-propanodGuo comparado aos moradores de região não poluída. Ademais, comprova-se pela primeira vez que células deficientes em reparo de ligações cruzadas apresentam níveis basais elevados de 1,N2-propanodGuo, em duas linhagens independentes, colocando este aduto como potencial mediador de carcinogênese em pacientes portadores de Anemia de Fanconi. Utilizando cérebro de ratos SOD1G93A (modelo de Esclerose Lateral Amiotrófica - ELA), verificou-se aumento de 50% nos níveis de 1,N2-propanodGuo e de 100% nos de 1,N6-εdAdo em fase sintomática, sugerindo influência do conteúdo lipídico cerebral, levando a comprometimento do metabolismo neuronal e morte celular. O perfil de aldeídos determinado em cérebro de ratos SOD1G93A demonstrou aumento de trans-hexa-2-enal e trans,trans-hexa-2,4-dienal em fase assintomática e de trans,trans-deca-2,4-dienal em fase sintomática, não sendo observada nenhuma alteração na medula. Conhecer estas variações permite direcionar estudos de modificações em biomoléculas, além de a metodologia per se corroborar com as áreas de análises lipidômicas. Técnicas distintas e o preparo de amostras refletiram nos níveis de glutationa reduzida (GSH) e oxidada (GSSG) relatados. A técnica de espectrometria de massas mostrou-se mais precisa que a detecção eletroquímica; e a alquilação do grupo tiol minimizou interferências de matriz. Por análise de HPLC-UV/Vis-ESI-MS/MS, a quantificação de trans-4-hidroxi-2-nonenal (HNE) e crotonaldeido conjugados com GSH demonstrou não haver alterações em cérebro e medula de ratos SOD1G93A. Contudo, há formação esteroespecífica dos adutos de HNE in vivo. Ressalta-se que a metodologia desenvolvida é extremamente sensível e específica e permite análise simultânea de GSH, GSSG, cisteína, cistina e dos adutos supracitados, servindo para… Advisors/Committee Members: Medeiros, Marisa Helena Gennari de.

Subjects/Keywords: Adutos de DNA; Adutos de glutationilação; Aldehydes; Aldeídos; Amyotrophic Lateral Sclerosis; Biomarcadores; Biomarkers; DNA adducts; Esclerose Lateral Amiotrófica; Estresse redox; Glutathionylation adducts; Stress redox

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Freitas, F. P. (2014). Investigação de potenciais biomarcadores redox - um enfoque em aldeídos e seus produtos. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/46/46131/tde-30092014-093431/ ;

Chicago Manual of Style (16^th Edition):

Freitas, Florêncio Porto. “Investigação de potenciais biomarcadores redox - um enfoque em aldeídos e seus produtos.” 2014. Doctoral Dissertation, University of São Paulo. Accessed January 17, 2021. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-30092014-093431/ ;.

MLA Handbook (7^th Edition):

Freitas, Florêncio Porto. “Investigação de potenciais biomarcadores redox - um enfoque em aldeídos e seus produtos.” 2014. Web. 17 Jan 2021.

Vancouver:

Freitas FP. Investigação de potenciais biomarcadores redox - um enfoque em aldeídos e seus produtos. [Internet] [Doctoral dissertation]. University of São Paulo; 2014. [cited 2021 Jan 17]. Available from: http://www.teses.usp.br/teses/disponiveis/46/46131/tde-30092014-093431/ ;.

Council of Science Editors:

Freitas FP. Investigação de potenciais biomarcadores redox - um enfoque em aldeídos e seus produtos. [Doctoral Dissertation]. University of São Paulo; 2014. Available from: http://www.teses.usp.br/teses/disponiveis/46/46131/tde-30092014-093431/ ;

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