subject:(Activit antiplasmodiale in vitro)

1. Cohen Potier de Courcy, Anita. Synthèse et évaluation antiparasitaire de nouveaux dérivés du thiazole et apparentés : Synthesis and antiparasitic evaluation of new thiazole derivatives and related structures.

Degree: Docteur es, Sciences, 2012, Aix Marseille Université

URL: http://www.theses.fr/2012AIXM5503

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L'objectif de ce travail consiste en la synthèse et l'évaluation antiparasitaire in vitro de nouveaux dérivés du thiazole et apparentés. Plusieurs stratégies de synthèse visant… (more)

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L'objectif de ce travail consiste en la synthèse et l'évaluation antiparasitaire in vitro de nouveaux dérivés du thiazole et apparentés. Plusieurs stratégies de synthèse visant à une pharmacomodulation en séries mono- et polycycliques ont été étudiées : en série 2-méthyl-5-nitrothiazole, la pharmacomodulation anti-Trichomonas de la position 4 par stratégie SRN1 n'a pas permis d'améliorer l'activité déjà démontrée en série 2-méthyl-5-nitroimidazole, mais a conduit à des dérivés à activité antiproliférative in vitro, spécifique de la lignée HepG2. En série 4-arylsulfonylméthyl-2-méthylthiazole, la pharmacomodulation de la position 5, par couplage de Suzuki-Miyaura d'une part, et par arylation directe et réaction de Knoevenagel intramoléculaire d'autre part, a conduit à des dérivés mono- et polycycliques dont certains ont démontré une activité antiplasmodiale in vitro encourageante. En série 5H-thiazolo[3,2-a]pyrimidin-5-one, la réaction de double couplage de Suzuki-Miyaura a révélé l'importance du groupement phényle en position 6 pour l'activité antiplasmodiale de ces dérivés. Enfin, l'évaluation biologique in vitro de thiéno[3,2-d]pyrimidin-4(3H)-ones a permis de caractériser le pharmacophore responsable de l'activité antiplasmodiale significative de cette série. Les résultats préliminaires encourageants d'une étude mécanistique antiplasmodiale présentent l'inhibition spécifique des kinases plasmodiales comme un mécanisme d'action potentiel de ces composés.

The objective of this work consists of the synthesis and the antiparasitic in vitro evaluation of new thiazole derivatives and related structures. Several synthetic strategies aiming at the pharmacomodulation on mono- and polycyclic series have been studied: in 2-methyl-5-nitrothiazole series, the anti-Trichomonas pharmacomodulation on position 4 by SRN1 strategy did not improve the activity previously demonstrated in 2-methyl-5-nitroimidazole series, but led to derivatives displaying a selective in vitro antiproliferative activity toward the HepG2 cell line. In 4-arylsulfonylmethyl-2-methylthiazole series, the pharmacomodulation on position 5, by Suzuki-Miyaura cross-coupling reaction on the one hand, and by direct arylation and intramolecular Knoevenagel reaction on the other hand, led to mono- and polycyclic derivatives among which some displayed an encouraging in vitro antiplasmodial activity. In 5H-thiazolo[3,2-a]pyrimidin-5-one series, a double Suzuki-Miyaura cross-coupling reaction revealed that the phenyl group on position 6 contributes to the antiplasmodial effect of this series. Finally, the in vitro biological evaluation of the thieno[3,2-d]pyrimidin-4(3H)-one scaffold let to characterize the pharmacophore responsible for the significant antiplasmodial activity. Some preliminary encouraging results regarding a mechanistic antiplasmodial study show the specific inhibition of plasmodial kinases, as a potential mechanism of action of some of these compounds.

Advisors/Committee Members: Vanelle, Patrice (thesis director), Rathelot, Pascal (thesis director).

Subjects/Keywords: Thiazole; Plasmodium falciparum; Activité antiproliférative in vitro; Activité antiplasmodiale in vitro; Pharmacomodulation; Kinases plasmodiales; Srn1; Palladium; Couplage de Suzuki-Miyaura; Arylation directe; Thiazole; Plasmodium falciparum; In vitro antiproliferative activity; In vitro antiplasmodial activity; Pharmacomodulation; Plasmodial kinases; Srn1; Palladium; Suzuki-Miyaura cross-coupling; Direct arylation

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APA (6^th Edition):

Cohen Potier de Courcy, A. (2012). Synthèse et évaluation antiparasitaire de nouveaux dérivés du thiazole et apparentés : Synthesis and antiparasitic evaluation of new thiazole derivatives and related structures. (Doctoral Dissertation). Aix Marseille Université. Retrieved from http://www.theses.fr/2012AIXM5503

Chicago Manual of Style (16^th Edition):

Cohen Potier de Courcy, Anita. “Synthèse et évaluation antiparasitaire de nouveaux dérivés du thiazole et apparentés : Synthesis and antiparasitic evaluation of new thiazole derivatives and related structures.” 2012. Doctoral Dissertation, Aix Marseille Université. Accessed January 21, 2021. http://www.theses.fr/2012AIXM5503.

MLA Handbook (7^th Edition):

Cohen Potier de Courcy, Anita. “Synthèse et évaluation antiparasitaire de nouveaux dérivés du thiazole et apparentés : Synthesis and antiparasitic evaluation of new thiazole derivatives and related structures.” 2012. Web. 21 Jan 2021.

Vancouver:

Cohen Potier de Courcy A. Synthèse et évaluation antiparasitaire de nouveaux dérivés du thiazole et apparentés : Synthesis and antiparasitic evaluation of new thiazole derivatives and related structures. [Internet] [Doctoral dissertation]. Aix Marseille Université 2012. [cited 2021 Jan 21]. Available from: http://www.theses.fr/2012AIXM5503.

Council of Science Editors:

Cohen Potier de Courcy A. Synthèse et évaluation antiparasitaire de nouveaux dérivés du thiazole et apparentés : Synthesis and antiparasitic evaluation of new thiazole derivatives and related structures. [Doctoral Dissertation]. Aix Marseille Université 2012. Available from: http://www.theses.fr/2012AIXM5503

2. Lemoine, Manuela. La réaction acrosomique du spermatozoïde chez le coq : The chicken acrosome reaction.

Degree: Docteur es, Sciences de la vie, 2009, Université François-Rabelais de Tours

URL: http://www.theses.fr/2009TOUR4005

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L’objectif de la thèse a été d’apporter des éléments sur la réaction acrosomique (RA) aviaire afin de mieux comprendre les processus menant à la fécondation… (more)

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L’objectif de la thèse a été d’apporter des éléments sur la réaction acrosomique (RA) aviaire afin de mieux comprendre les processus menant à la fécondation et de mieux maîtriser la capacité des spermatozoïdes à être conservés. Nos résultats ont conforté l’hypothèse de l’absence de capacitation chez les oiseaux. De plus, il n’y a pas d’hyperactivation de la mobilité lors de la RA. Seul le Ca2+ s’avère être l’élément indispensable au déclenchement de la RA. L’évaluation de la RA avec des spermatozoïdes conservés à l’état liquide ou après cryoconservation a révélé une évolution différente en fonction du type de conservation. L’étude des voies de signalisation susceptibles d’être impliquées dans le déclenchement de la RA a suggéré l’activation de 3voies, PKA, PI3K et MAPK ERK. Ce travail ouvre de nombreuses perspectives scientifiques vers l’approfondissement des connaissances de la RA chez les oiseaux et sur l’utilisation qui peut en être faite pour mieux maîtriser la qualité des gamètes.

The aim of this work was to provide new information on chicken acrosome reaction (AR) for a better comprehension of the mechanisms leading to this reaction and a better control of the fertilizing potential of spermatozoa after in vitro storage. Our results showed that calcium is the factor absolutely necessary to initiate the AR and supported the hypothesis that chicken spermatozoa do not need to be capacitated. Moreover, motility hyperactivation was not found at the time of AR. Then, we showed that chicken sperm ability to undergo the AR may differ depending on the type of semen storage. Indeed, this ability was dramatically affected by liquid storage, but was submitted to contrasted effect after cryopreservation. Finally, we investigated the potential involvement of several signaling pathways in initiation of the chicken AR and the results showed that the AR could be mediated by activation of the PKA, PI3K and ERK MAPK pathways.

Advisors/Committee Members: Blesbois, Elisabeth (thesis director).

Subjects/Keywords: Conservation in vitro

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APA (6^th Edition):

Lemoine, M. (2009). La réaction acrosomique du spermatozoïde chez le coq : The chicken acrosome reaction. (Doctoral Dissertation). Université François-Rabelais de Tours. Retrieved from http://www.theses.fr/2009TOUR4005

Chicago Manual of Style (16^th Edition):

Lemoine, Manuela. “La réaction acrosomique du spermatozoïde chez le coq : The chicken acrosome reaction.” 2009. Doctoral Dissertation, Université François-Rabelais de Tours. Accessed January 21, 2021. http://www.theses.fr/2009TOUR4005.

MLA Handbook (7^th Edition):

Lemoine, Manuela. “La réaction acrosomique du spermatozoïde chez le coq : The chicken acrosome reaction.” 2009. Web. 21 Jan 2021.

Vancouver:

Lemoine M. La réaction acrosomique du spermatozoïde chez le coq : The chicken acrosome reaction. [Internet] [Doctoral dissertation]. Université François-Rabelais de Tours; 2009. [cited 2021 Jan 21]. Available from: http://www.theses.fr/2009TOUR4005.

Council of Science Editors:

Lemoine M. La réaction acrosomique du spermatozoïde chez le coq : The chicken acrosome reaction. [Doctoral Dissertation]. Université François-Rabelais de Tours; 2009. Available from: http://www.theses.fr/2009TOUR4005

Universiteit Utrecht

3. Marlen, R.L.M. van. Acclimatization, survival and growth of in vitro grown Arabidopsis thaliana seedlings after transplanting to soil.

Degree: 2015, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/317684

► Plants grown in vitro have a different morphology, physiology and anatomy than plants grown in soil. In vitro grown plants usually have non-functional stomata, a… (more)

Subjects/Keywords: Arabidopsis thaliana; acclimatization; in vitro

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APA (6^th Edition):

Marlen, R. L. M. v. (2015). Acclimatization, survival and growth of in vitro grown Arabidopsis thaliana seedlings after transplanting to soil. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/317684

Chicago Manual of Style (16^th Edition):

Marlen, R L M van. “Acclimatization, survival and growth of in vitro grown Arabidopsis thaliana seedlings after transplanting to soil.” 2015. Masters Thesis, Universiteit Utrecht. Accessed January 21, 2021. http://dspace.library.uu.nl:8080/handle/1874/317684.

MLA Handbook (7^th Edition):

Marlen, R L M van. “Acclimatization, survival and growth of in vitro grown Arabidopsis thaliana seedlings after transplanting to soil.” 2015. Web. 21 Jan 2021.

Vancouver:

Marlen RLMv. Acclimatization, survival and growth of in vitro grown Arabidopsis thaliana seedlings after transplanting to soil. [Internet] [Masters thesis]. Universiteit Utrecht; 2015. [cited 2021 Jan 21]. Available from: http://dspace.library.uu.nl:8080/handle/1874/317684.

Council of Science Editors:

Marlen RLMv. Acclimatization, survival and growth of in vitro grown Arabidopsis thaliana seedlings after transplanting to soil. [Masters Thesis]. Universiteit Utrecht; 2015. Available from: http://dspace.library.uu.nl:8080/handle/1874/317684

4. Kelle de Andrade Lemoine Neves, Juliana. Imidazolidinas esquistomicidas: avaliação ultraestrutural, atividade citotóxica e imunomoduladora .

Degree: 2010, Universidade Federal de Pernambuco

URL: http://repositorio.ufpe.br/handle/123456789/1273

► A esquistossomose é uma doença debilitante e endêmica, distribuída em 74 países, causada por parasitas do gênero Schistosoma, onde existem cinco espécies de interesse médico,… (more)

Subjects/Keywords: Esquistossomose; Imidazolidinadionas; Ensaios in vitro

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APA (6^th Edition):

Kelle de Andrade Lemoine Neves, J. (2010). Imidazolidinas esquistomicidas: avaliação ultraestrutural, atividade citotóxica e imunomoduladora . (Thesis). Universidade Federal de Pernambuco. Retrieved from http://repositorio.ufpe.br/handle/123456789/1273

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kelle de Andrade Lemoine Neves, Juliana. “Imidazolidinas esquistomicidas: avaliação ultraestrutural, atividade citotóxica e imunomoduladora .” 2010. Thesis, Universidade Federal de Pernambuco. Accessed January 21, 2021. http://repositorio.ufpe.br/handle/123456789/1273.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kelle de Andrade Lemoine Neves, Juliana. “Imidazolidinas esquistomicidas: avaliação ultraestrutural, atividade citotóxica e imunomoduladora .” 2010. Web. 21 Jan 2021.

Vancouver:

Kelle de Andrade Lemoine Neves J. Imidazolidinas esquistomicidas: avaliação ultraestrutural, atividade citotóxica e imunomoduladora . [Internet] [Thesis]. Universidade Federal de Pernambuco; 2010. [cited 2021 Jan 21]. Available from: http://repositorio.ufpe.br/handle/123456789/1273.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kelle de Andrade Lemoine Neves J. Imidazolidinas esquistomicidas: avaliação ultraestrutural, atividade citotóxica e imunomoduladora . [Thesis]. Universidade Federal de Pernambuco; 2010. Available from: http://repositorio.ufpe.br/handle/123456789/1273

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

5. Mansseri née Lamrioui, Akila. Etude de la multiplicatoin par clonage "in vitro" du merisier (Prunus avium L.).

Degree: 2011, Université M'Hamed Bougara Boumerdès

URL: http://dlibrary.univ-boumerdes.dz:8080/handle/123456789/103

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161 p. , ill. , 30 cm

Le présent travail a porté sur l’étude de la multiplication du Merisier (Prunus avium L.) par clonage in… (more)

Subjects/Keywords: Clonage; Multiplication in vitro; Merisier

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APA (6^th Edition):

Mansseri née Lamrioui, A. (2011). Etude de la multiplicatoin par clonage "in vitro" du merisier (Prunus avium L.). (Thesis). Université M'Hamed Bougara Boumerdès. Retrieved from http://dlibrary.univ-boumerdes.dz:8080/handle/123456789/103

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mansseri née Lamrioui, Akila. “Etude de la multiplicatoin par clonage "in vitro" du merisier (Prunus avium L.).” 2011. Thesis, Université M'Hamed Bougara Boumerdès. Accessed January 21, 2021. http://dlibrary.univ-boumerdes.dz:8080/handle/123456789/103.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mansseri née Lamrioui, Akila. “Etude de la multiplicatoin par clonage "in vitro" du merisier (Prunus avium L.).” 2011. Web. 21 Jan 2021.

Vancouver:

Mansseri née Lamrioui A. Etude de la multiplicatoin par clonage "in vitro" du merisier (Prunus avium L.). [Internet] [Thesis]. Université M'Hamed Bougara Boumerdès; 2011. [cited 2021 Jan 21]. Available from: http://dlibrary.univ-boumerdes.dz:8080/handle/123456789/103.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mansseri née Lamrioui A. Etude de la multiplicatoin par clonage "in vitro" du merisier (Prunus avium L.). [Thesis]. Université M'Hamed Bougara Boumerdès; 2011. Available from: http://dlibrary.univ-boumerdes.dz:8080/handle/123456789/103

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

6. Renzi, Adriana. Análise da influência do hormônio anti-Mülleriano na produção in vitro de embriões bovinos.

Degree: PhD, Genética, 2012, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/17/17135/tde-19042013-154338/ ;

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A maturação in vitro de oócitos (MIV) é uma importante tecnologia reprodutiva a qual gera oócitos maduros capazes de suportar o desenvolvimento embrionário pré-implantacional e… (more)

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A maturação in vitro de oócitos (MIV) é uma importante tecnologia reprodutiva a qual gera oócitos maduros capazes de suportar o desenvolvimento embrionário pré-implantacional e sua completa evolução à termo. Muitos fatores levam ao processo de maturação do oócito, e o AMH (hormônio anti-Mülleriano) tem demonstrado possuir um importante efeito nesta etapa. Neste trabalho nós demonstramos a influência da suplementação de AMH na maturação de complexos cumulus-oócito (COCs). Nossos resultados demonstram que não houve efeito na produção de embriões para COCS grau I. Entretanto, pudemos encontrar diferenças significativas entre os COCs graus II e III maturados na presença de 150ng/ml de AMH. Aqui também demonstramos que não houve diferença significativa na expressão relativa de mRNA para os genes AMHRII e FSHR no oócito, e na expressão relativa de mRNA para os genes AMH, AMHRII e FSHR nas células da granulosa. Nossos resultados corroboram com as importantes funções do AMH na produção de embriões, sugerem que a suplementação do meio de maturação de oócitos com AMH pode ajudar a melhorar a produção de blastocistos.

The in vitro oocyte maturation (MIV) is an important reproductive technology that generates mature oocytes able to support the preimplantation embryonic development and their fully evolution to term. Many factors lead to oocyte maturation process, and the AMH (AntiMüllerian hormone) have demonstrated important effects in the oocyte development. Here, we report the influence of AMH supplementation in the cumulus-oocyte complex (COCs) maturation. We found that AMH had no effect on embryo production of COCs grade I. On the other hand, significant differences between the COCs grade II and COCs grade III matured in AMH 150ng/ml were verified. We have also demonstrated that there were no significant difference in mRNA expression of the genes AMHRII and FSHR in the oocyte, and in mRNA of the genes AMH, AMHRII and FSHR in the granulosa cells. Taken together, the results corroborate the important roles for AMH on embryo production, and suggest that AMH supplementation could achieve successful outcome in the production of blastocysts.

Advisors/Committee Members: Lôbo, Raysildo Barbosa.

Subjects/Keywords: Anti-Müllerian hormone.; Bovinos; Cattle; Fertilização In Vitro; Hormônio Anti-Mülleriano.; In Vitro Fertilization; In Vitro Maturation; Maturação In Vitro

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APA (6^th Edition):

Renzi, A. (2012). Análise da influência do hormônio anti-Mülleriano na produção in vitro de embriões bovinos. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/17/17135/tde-19042013-154338/ ;

Chicago Manual of Style (16^th Edition):

Renzi, Adriana. “Análise da influência do hormônio anti-Mülleriano na produção in vitro de embriões bovinos.” 2012. Doctoral Dissertation, University of São Paulo. Accessed January 21, 2021. http://www.teses.usp.br/teses/disponiveis/17/17135/tde-19042013-154338/ ;.

MLA Handbook (7^th Edition):

Renzi, Adriana. “Análise da influência do hormônio anti-Mülleriano na produção in vitro de embriões bovinos.” 2012. Web. 21 Jan 2021.

Vancouver:

Renzi A. Análise da influência do hormônio anti-Mülleriano na produção in vitro de embriões bovinos. [Internet] [Doctoral dissertation]. University of São Paulo; 2012. [cited 2021 Jan 21]. Available from: http://www.teses.usp.br/teses/disponiveis/17/17135/tde-19042013-154338/ ;.

Council of Science Editors:

Renzi A. Análise da influência do hormônio anti-Mülleriano na produção in vitro de embriões bovinos. [Doctoral Dissertation]. University of São Paulo; 2012. Available from: http://www.teses.usp.br/teses/disponiveis/17/17135/tde-19042013-154338/ ;

Cornell University

7. Blakeley, Amy. Understanding The Effect Of Interface Chemistry In Biomineralization – Development Of An In Vitro Model For Calcium Phosphate Mineralization.

Degree: PhD, Chemistry and Chemical Biology, 2013, Cornell University

URL: http://hdl.handle.net/1813/33811

► Structure-function relationships in mineralized tissues, such as bone and teeth, depend upon the chemical interactions of the interfaces between cells, proteins, organic matrix, and mineral… (more)

▼ Structure-function relationships in mineralized tissues, such as bone and teeth, depend upon the chemical interactions of the interfaces between cells, proteins, organic matrix, and mineral (calcium phosphate). Development of in vitro models that more accurately model mineralization systems in vivo will assist in understanding medical conditions as well as inform biomimetic materials development. Understanding the heirachical structures in mineralized tissues relies on the chemical control of crystal nucleation and growth at the interfaces. Studies presented in this thesis provide additional in vitro tools for studying in vivo systems by controlling for interface chemistry, mineralization environment, and experimental setup for elucidating the effect of interfaces in biomineralization. Covalently bound surface chemistry - oxide, methyl, carboxylate, 2-aminoethyl dihydrogen phosphate, osteopontin, and bovine serum albumin (BSA) - on porous silicon substrates has a distinct affect on surface mineral nucleation and morphology. The morphology and coverage resulting from suspending the differed from those reported in literature. This method has potential for selectively examining the effects of covalently bound protein conformations and segments, more closely mimicking the conformation constraints at interfaces in biological systems. Hydrogel-based double diffusion system (DDS) has the potential to allow for the simultaneous in vitro study of cell-mineral-matrix interactions in a 3-D environment, the 'holy-grail' of mineralization studies. Successful hydroxyapatite mineralization was demonstrated at both room temperature and incubator conditions using a custom build polycarbonate DDS with polydimethylsiloxane membranes for gas permeability, minimal polyethyleneimine coating for gel adhesion, and cell culture compatible Agarose. This system provides researchers with a new tool for combining cell, mineral, and matrix in a 3-D environment for understanding the complexity of cell-mineral-matrix interactions. Understanding how proteins interact with surface chemistry as well as their structural changes and coverage, provides important clues towards biology's ability to exert control over biomineralization. Analysis of the monolayer-protein interface via infrared spectroscopy demonstrated that the protein conformation increases in disorder upon adsorption to the surface based on amide peak positions and amounts of adsorbed protein varied as a function of chemistry. Work in this thesis provides tools and information towards developing in vitro systems that elucidate complex interactions in mineralized tissues. Advisors/Committee Members: Ober, Christopher Kemper (chair), Van Dover, Robert B. (committee member), Estroff, Lara A. (committee member).

Subjects/Keywords: Biomineralization; interfaces; in vitro models

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APA (6^th Edition):

Blakeley, A. (2013). Understanding The Effect Of Interface Chemistry In Biomineralization – Development Of An In Vitro Model For Calcium Phosphate Mineralization. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/33811

Chicago Manual of Style (16^th Edition):

Blakeley, Amy. “Understanding The Effect Of Interface Chemistry In Biomineralization – Development Of An In Vitro Model For Calcium Phosphate Mineralization.” 2013. Doctoral Dissertation, Cornell University. Accessed January 21, 2021. http://hdl.handle.net/1813/33811.

MLA Handbook (7^th Edition):

Blakeley, Amy. “Understanding The Effect Of Interface Chemistry In Biomineralization – Development Of An In Vitro Model For Calcium Phosphate Mineralization.” 2013. Web. 21 Jan 2021.

Vancouver:

Blakeley A. Understanding The Effect Of Interface Chemistry In Biomineralization – Development Of An In Vitro Model For Calcium Phosphate Mineralization. [Internet] [Doctoral dissertation]. Cornell University; 2013. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/1813/33811.

Council of Science Editors:

Blakeley A. Understanding The Effect Of Interface Chemistry In Biomineralization – Development Of An In Vitro Model For Calcium Phosphate Mineralization. [Doctoral Dissertation]. Cornell University; 2013. Available from: http://hdl.handle.net/1813/33811

University of Georgia

8. Dew, Elizabeth Virginia. In vitro maturation of the canine oocyte.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/20311

► This research examines culture techniques to improve nuclear maturation of canine oocytes in vitro.The primary objective of these experiments was to test individual;1)estradiol or progesterone,2)FSH,3)eCG,4)LH,5)L-cysteine,6)… (more)

Subjects/Keywords: Canine; in vitro maturation; occyte

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APA (6^th Edition):

Dew, E. V. (2014). In vitro maturation of the canine oocyte. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/20311

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dew, Elizabeth Virginia. “In vitro maturation of the canine oocyte.” 2014. Thesis, University of Georgia. Accessed January 21, 2021. http://hdl.handle.net/10724/20311.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dew, Elizabeth Virginia. “In vitro maturation of the canine oocyte.” 2014. Web. 21 Jan 2021.

Vancouver:

Dew EV. In vitro maturation of the canine oocyte. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/10724/20311.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dew EV. In vitro maturation of the canine oocyte. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/20311

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

9. Mansseri née Lamrioui, Akila. Etude de la multiplicatoin par clonage "in vitro" du merisier (Prunus avium L.).

Degree: 2011, Université M'Hamed Bougara Boumerdès

URL: http://dlibrary.univ-boumerdes.dz:8080/jspui/handle/123456789/174

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161 p. , ill. , 30 cm

Le présent travail a porté sur l’étude de la multiplication du Merisier (Prunus avium L.) par clonage in… (more)

Subjects/Keywords: Clonage; Multiplication in vitro; Merisier

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mansseri née Lamrioui, A. (2011). Etude de la multiplicatoin par clonage "in vitro" du merisier (Prunus avium L.). (Thesis). Université M'Hamed Bougara Boumerdès. Retrieved from http://dlibrary.univ-boumerdes.dz:8080/jspui/handle/123456789/174

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mansseri née Lamrioui, Akila. “Etude de la multiplicatoin par clonage "in vitro" du merisier (Prunus avium L.).” 2011. Thesis, Université M'Hamed Bougara Boumerdès. Accessed January 21, 2021. http://dlibrary.univ-boumerdes.dz:8080/jspui/handle/123456789/174.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mansseri née Lamrioui, Akila. “Etude de la multiplicatoin par clonage "in vitro" du merisier (Prunus avium L.).” 2011. Web. 21 Jan 2021.

Vancouver:

Mansseri née Lamrioui A. Etude de la multiplicatoin par clonage "in vitro" du merisier (Prunus avium L.). [Internet] [Thesis]. Université M'Hamed Bougara Boumerdès; 2011. [cited 2021 Jan 21]. Available from: http://dlibrary.univ-boumerdes.dz:8080/jspui/handle/123456789/174.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mansseri née Lamrioui A. Etude de la multiplicatoin par clonage "in vitro" du merisier (Prunus avium L.). [Thesis]. Université M'Hamed Bougara Boumerdès; 2011. Available from: http://dlibrary.univ-boumerdes.dz:8080/jspui/handle/123456789/174

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas A&M University

10. Klopatek, Sarah B. Ruminant Methanogenic Activity in the United States Beef Cattle Industry.

Degree: MS, Animal Science, 2016, Texas A&M University

URL: http://hdl.handle.net/1969.1/157878

► In the U.S., gastrointestinal fermentation from cattle is estimated to account for approximately 25% of total anthropogenic related methane (CH4) emissions. In addition, 2-8% of… (more)

▼ In the U.S., gastrointestinal fermentation from cattle is estimated to account for approximately 25% of total anthropogenic related methane (CH4) emissions. In addition, 2-8% of gross energy consumed by cattle is lost in the form of CH4, representing an energetic cost to the animal. Thereby, in order to decrease greenhouse gas emissions (GHG) and improve the efficiency of cattle production additional research on gastrointestinal CH4 emissions from cattle is needed. In ruminants carbohydrate (CHO) catabolism and nitrogen (N) utilization have a tremendous impact on ruminal methanogenesis. However, the impact of purified carbohydrates in the presence of a variety of N sources on rates of CH4 and VFA production remains unknown. In order to determine these rates for use in predictive models of the ruminal fermentation, we formulated a fractional rate equation to fit the rate of CH4 production and measured the concentration of CH4 and VFA and using purified CHO with a variety of N sources in two in vitro mixed ruminal microorganism fermentation studies. In both studies, a CHO treatment × incubation time (IT) effect was observed for both VFA and CH4 (P < 0.01). There was also an N × IT interaction for CH4 production at 24 h in Study 2, where nitrogen free and NH3 treatments produced greater concentrations of CH4 than treatments with amino acids (P < 0.01). A nonlinear equation for the conversion of carbohydrates to CH4 was able to fit starch treatments in Study 1 and glucose treatments in Study 2. Overall, this study demonstrated different fermentation patterns among all CHO and N sources and was the first step in determining rates for in vitro CH4 production. Although cattle contribute with high amounts of anthropologic GHG, they are not the only methanogenic producing food source in the U.S. Rice and wild ruminants (e.g. bison, elk, and deer) are also methanogenic producing food sources. The objective of this final study was to compare the efficiency of beef and milk production to pre-settlement wild ruminants and rice production on a kilogram of CH4 emitted to kilogram human-edible protein production basis. Bison had the highest ratio of 13.93 kg CH4: Protein, followed by elk (12.50) deer (6.66) and beef (2.47). Overall, wild ruminants emitted 296 to 564 percent more CH4 per kilogram of human-edible protein produced than current beef cattle production systems. Rice yielded the second lowest CH4 to human-edible protein ratio (0.83), followed by dairy cattle milk production (0.50). We believe, this analysis provides insight on the efficiency of methanogenic food sources that may aid in the development of regulatory guidelines of CH4 production. Advisors/Committee Members: Wickersham, Tryon (advisor), Tedeschi, Luis (advisor), Callaway, Todd (committee member), Sawyer, Jason (committee member).

Subjects/Keywords: Ruminants; Methane; in vitro; Sustainability

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APA (6^th Edition):

Klopatek, S. B. (2016). Ruminant Methanogenic Activity in the United States Beef Cattle Industry. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/157878

Chicago Manual of Style (16^th Edition):

Klopatek, Sarah B. “Ruminant Methanogenic Activity in the United States Beef Cattle Industry.” 2016. Masters Thesis, Texas A&M University. Accessed January 21, 2021. http://hdl.handle.net/1969.1/157878.

MLA Handbook (7^th Edition):

Klopatek, Sarah B. “Ruminant Methanogenic Activity in the United States Beef Cattle Industry.” 2016. Web. 21 Jan 2021.

Vancouver:

Klopatek SB. Ruminant Methanogenic Activity in the United States Beef Cattle Industry. [Internet] [Masters thesis]. Texas A&M University; 2016. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/1969.1/157878.

Council of Science Editors:

Klopatek SB. Ruminant Methanogenic Activity in the United States Beef Cattle Industry. [Masters Thesis]. Texas A&M University; 2016. Available from: http://hdl.handle.net/1969.1/157878

11. Kang, Hyeran. Kinetic and Kinematic Properties of Actin Network Assembly and Nonlinearity of Cross-Linked Gels.

Degree: PhD, Physics, 2009, Brown University

URL: https://repository.library.brown.edu/studio/item/bdr:232/

► Although recent studies have identified the biochemical components required to generate motile forces by actin polymerization, how the forces are generated remains unclear. To elucidate… (more)

▼ Although recent studies have identified the biochemical components required to generate motile forces by actin polymerization, how the forces are generated remains unclear. To elucidate this molecular mechanism of cell motility, I investigated the biophysical conditions under which actin-based growth and motility take place. In Part I of this dissertation, I report an experimental study on the kinetics of actin assembly mediated by branching and capping proteins. The experiments were performed by fluorescence imaging and light-scattering intensity measurement. The findings confirm the recent theoretical prediction that a "branching explosion" occurs during polymerization. Furthermore, the branching explosion occurs over a limited range of the ratio between branching protein and capping protein concentrations. This is also consistent with the theoretical model. These results establish a natural link between the kinetic theory of actin assembly in vitro and the cytoskeletal structure and actin dynamics in motile cells. In Part II of this dissertation, I present an in vitro study of the actin-based movement of functionalized spherical beads in comparison with those of bacteria like Listeria. Long trajectories induced by the spherical beads show characteristic differences with those observed for bacteria, which have both an elongated shape and an asymmetric expression of the polymerization inducing enzyme. The experimental trajectories can be simulated using a generalized kinematic model that includes the rotation of the bead relative to the actin tail. These results imply that the trajectories of spherical beads are mechanically deterministic rather than random, as suggested in stochastic models. In Part III of this dissertation, I examine nonlinear viscoelasticity of cross-linked biopolymer networks, by rheological measurements of shear moduli and normal stresses in fibrin gels. The results for coarse fibrin gels are consistent with expectations from theories of rod-like filament networks. Comparison of rheological and optical properties shows that the filament alignment, as measured by optical retardance increases with increasing shear strain but lags behind the increase in shear moduli. Advisors/Committee Members: Tang, Jay (director), Stein, Derek (reader), Shenoy, Vivek (reader), Shaw, Sunil (reader).

Subjects/Keywords: in vitro actin assembly

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APA (6^th Edition):

Kang, H. (2009). Kinetic and Kinematic Properties of Actin Network Assembly and Nonlinearity of Cross-Linked Gels. (Doctoral Dissertation). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:232/

Chicago Manual of Style (16^th Edition):

Kang, Hyeran. “Kinetic and Kinematic Properties of Actin Network Assembly and Nonlinearity of Cross-Linked Gels.” 2009. Doctoral Dissertation, Brown University. Accessed January 21, 2021. https://repository.library.brown.edu/studio/item/bdr:232/.

MLA Handbook (7^th Edition):

Kang, Hyeran. “Kinetic and Kinematic Properties of Actin Network Assembly and Nonlinearity of Cross-Linked Gels.” 2009. Web. 21 Jan 2021.

Vancouver:

Kang H. Kinetic and Kinematic Properties of Actin Network Assembly and Nonlinearity of Cross-Linked Gels. [Internet] [Doctoral dissertation]. Brown University; 2009. [cited 2021 Jan 21]. Available from: https://repository.library.brown.edu/studio/item/bdr:232/.

Council of Science Editors:

Kang H. Kinetic and Kinematic Properties of Actin Network Assembly and Nonlinearity of Cross-Linked Gels. [Doctoral Dissertation]. Brown University; 2009. Available from: https://repository.library.brown.edu/studio/item/bdr:232/

Addis Ababa University

12. ASKALE, GIZAW. IN VITRO EFFECACY OF METHANOLIC EXTRACTS OF VERNONIA AMYGDALINA, CROTON MACROSTACHYUS, RICINUS COMMUNIS AND PETROLEUM ETHER EXTRACT OF MILLETTIA FERRUGINEA AGAINST BOVICOLA OVIS AND RHIPICEPHALUS (BOOPHILUS) DECOLORATUS .

Degree: 2015, Addis Ababa University

URL: http://etd.aau.edu.et/dspace/handle/123456789/7794

► In vitro loucicidal and accaricidal efficacy evaluation of Vernonia amygdalina, Croton macrostachyus, Ricinus communis and Millettia ferruginea against Bovicola ovis and Rhipicephalus decoloratus of cattle… (more)

▼ In vitro loucicidal and accaricidal efficacy evaluation of Vernonia amygdalina, Croton macrostachyus, Ricinus communis and Millettia ferruginea against Bovicola ovis and Rhipicephalus decoloratus of cattle were carried out from January 2014 to April 2015. Crude methanol extracts of Vernonia amygdalina, Croton macrostachyus, Ricinus communis leaf and Petroleum ether extract of Millettia ferruginea seed oil were prepared for in vitro test at different time intervals. The four selected medicinal plants, at concentrations of 200, 100, 50, 25, 12.5 and 6.25mg/ml and a commercially used acaricide (0.1% diazinon), were examined using in vitro adult immersion test. In vitro loucicidal test showed all concentration of Millettia ferruginea oil (µl/ml), ≥25mg /ml concentration of Croton macrostachyus and 200 and 100mg/ml concentration of Ricinus communis had pronounced loucidal activity at 24hr of exposure. 200mg/ml concentration of Vernonia amygdalina and ≤6.25mg/ml concentration of Croton macr ostachyus had moderate loucicidal activity at 24hr of exposure. Moreover, the 200 and 100µl/ml concentration of Millettia ferruginea seed oil and 200mg/ml concentration of Ricinus communis leaf crude extract had high accaricidal activity (90-100%) against Rhipicephalus decoloratus while crude methanolic extracts of Croton macrostachyus and Vernonia amygdalina and even the positive control (diazinon) showed low acaricidal activity (<35%) against the tick species. All plants had significantly (P< 0.05) higher activity against lice than Rhipicephalus decoloratus ticks except Millettia ferruginea at (200 and 100µl/ml) and Ricinus communis (at 200 mg/ml) which showed both accaricidal and loucicidal activity. Therefore the present study concluded that Croton macrostachyus, Ricinus communis and Millettia ferruginea against Bovicola ovis whereas Ricinus communis and Millettia ferruginea against Rhipicephalus decoloratus could be used as potential alternative in the discovery of guide compounds that substitute commercially available acaricides. In addition, the low activity of diazinon on ticks compared to our plant extracts deserves further attention. Advisors/Committee Members: Dr. Yacob Hailu (advisor).

Subjects/Keywords: Acaricide; Bovicola ovis; in vitro

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APA (6^th Edition):

ASKALE, G. (2015). IN VITRO EFFECACY OF METHANOLIC EXTRACTS OF VERNONIA AMYGDALINA, CROTON MACROSTACHYUS, RICINUS COMMUNIS AND PETROLEUM ETHER EXTRACT OF MILLETTIA FERRUGINEA AGAINST BOVICOLA OVIS AND RHIPICEPHALUS (BOOPHILUS) DECOLORATUS . (Thesis). Addis Ababa University. Retrieved from http://etd.aau.edu.et/dspace/handle/123456789/7794

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

ASKALE, GIZAW. “IN VITRO EFFECACY OF METHANOLIC EXTRACTS OF VERNONIA AMYGDALINA, CROTON MACROSTACHYUS, RICINUS COMMUNIS AND PETROLEUM ETHER EXTRACT OF MILLETTIA FERRUGINEA AGAINST BOVICOLA OVIS AND RHIPICEPHALUS (BOOPHILUS) DECOLORATUS .” 2015. Thesis, Addis Ababa University. Accessed January 21, 2021. http://etd.aau.edu.et/dspace/handle/123456789/7794.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

ASKALE, GIZAW. “IN VITRO EFFECACY OF METHANOLIC EXTRACTS OF VERNONIA AMYGDALINA, CROTON MACROSTACHYUS, RICINUS COMMUNIS AND PETROLEUM ETHER EXTRACT OF MILLETTIA FERRUGINEA AGAINST BOVICOLA OVIS AND RHIPICEPHALUS (BOOPHILUS) DECOLORATUS .” 2015. Web. 21 Jan 2021.

Vancouver:

ASKALE G. IN VITRO EFFECACY OF METHANOLIC EXTRACTS OF VERNONIA AMYGDALINA, CROTON MACROSTACHYUS, RICINUS COMMUNIS AND PETROLEUM ETHER EXTRACT OF MILLETTIA FERRUGINEA AGAINST BOVICOLA OVIS AND RHIPICEPHALUS (BOOPHILUS) DECOLORATUS . [Internet] [Thesis]. Addis Ababa University; 2015. [cited 2021 Jan 21]. Available from: http://etd.aau.edu.et/dspace/handle/123456789/7794.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

ASKALE G. IN VITRO EFFECACY OF METHANOLIC EXTRACTS OF VERNONIA AMYGDALINA, CROTON MACROSTACHYUS, RICINUS COMMUNIS AND PETROLEUM ETHER EXTRACT OF MILLETTIA FERRUGINEA AGAINST BOVICOLA OVIS AND RHIPICEPHALUS (BOOPHILUS) DECOLORATUS . [Thesis]. Addis Ababa University; 2015. Available from: http://etd.aau.edu.et/dspace/handle/123456789/7794

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Guelph

13. Lau, Wilson. A Study of the In Vitro Bioaccessibilities of Nickel Compounds in Particulate Form as they are Affected by Variation in Assay Conditions.

Degree: MS, Department of Land Resource Science, 2012, University of Guelph

URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3953

► In vitro bioaccessibility of Ni from NiO, NiSO4, Ni3S2, NiS, and Ni metal in the absence of soil was measured under assay conditions known to… (more)

Subjects/Keywords: Nickel; bioaccessibility; bioavailability; in vitro

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APA (6^th Edition):

Lau, W. (2012). A Study of the In Vitro Bioaccessibilities of Nickel Compounds in Particulate Form as they are Affected by Variation in Assay Conditions. (Masters Thesis). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3953

Chicago Manual of Style (16^th Edition):

Lau, Wilson. “A Study of the In Vitro Bioaccessibilities of Nickel Compounds in Particulate Form as they are Affected by Variation in Assay Conditions.” 2012. Masters Thesis, University of Guelph. Accessed January 21, 2021. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3953.

MLA Handbook (7^th Edition):

Lau, Wilson. “A Study of the In Vitro Bioaccessibilities of Nickel Compounds in Particulate Form as they are Affected by Variation in Assay Conditions.” 2012. Web. 21 Jan 2021.

Vancouver:

Lau W. A Study of the In Vitro Bioaccessibilities of Nickel Compounds in Particulate Form as they are Affected by Variation in Assay Conditions. [Internet] [Masters thesis]. University of Guelph; 2012. [cited 2021 Jan 21]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3953.

Council of Science Editors:

Lau W. A Study of the In Vitro Bioaccessibilities of Nickel Compounds in Particulate Form as they are Affected by Variation in Assay Conditions. [Masters Thesis]. University of Guelph; 2012. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3953

14. Afonso, Ricardo. Estudos sobre aproveitamento biotecnológico da quitina produzida por organismos marinhos.

Degree: 2008, RCAAP

URL: http://www.rcaap.pt/detail.jsp?id=oai:sapientia.ualg.pt:10400.1/710

►

Dissertação mest., Biotecnologia, Universidade do Algarve, 2008

O oceano é um dos mais ricos habitats do planeta, mas também um dos menos explorados, embora a… (more)

Subjects/Keywords: Teses; Biotecnologia; Cultura in vitro

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APA (6^th Edition):

Afonso, R. (2008). Estudos sobre aproveitamento biotecnológico da quitina produzida por organismos marinhos. (Thesis). RCAAP. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:sapientia.ualg.pt:10400.1/710

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Afonso, Ricardo. “Estudos sobre aproveitamento biotecnológico da quitina produzida por organismos marinhos.” 2008. Thesis, RCAAP. Accessed January 21, 2021. http://www.rcaap.pt/detail.jsp?id=oai:sapientia.ualg.pt:10400.1/710.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Afonso, Ricardo. “Estudos sobre aproveitamento biotecnológico da quitina produzida por organismos marinhos.” 2008. Web. 21 Jan 2021.

Vancouver:

Afonso R. Estudos sobre aproveitamento biotecnológico da quitina produzida por organismos marinhos. [Internet] [Thesis]. RCAAP; 2008. [cited 2021 Jan 21]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:sapientia.ualg.pt:10400.1/710.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Afonso R. Estudos sobre aproveitamento biotecnológico da quitina produzida por organismos marinhos. [Thesis]. RCAAP; 2008. Available from: http://www.rcaap.pt/detail.jsp?id=oai:sapientia.ualg.pt:10400.1/710

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

15. Calheiros, Ana Manuel Casanova de Girão. Relação ácido-base na absorção de fármacos.

Degree: 2013, RCAAP

URL: https://www.rcaap.pt/detail.jsp?id=oai:comum.rcaap.pt:10400.26/14121

►

Dissertação para obtenção do grau de Mestre no Instituto Superior de Ciências da Saúde Egas Moniz

Neste trabalho são apresentados conceitos sobre a absorção de… (more)

▼

Dissertação para obtenção do grau de Mestre no Instituto Superior de Ciências da Saúde Egas Moniz

Neste trabalho são apresentados conceitos sobre a absorção de fármacos e em que aspecto a relação ácido-base a pode influenciar. A absorção consiste na transferência do fármaco do local de administração até à corrente sanguínea. Os fármacos podem ser administrados por diversas vias, sendo a via oral a mais destacada nesta dissertação, devido à grande variação de pH ao longo do TGI (de 1.3 a 7.5), o que implica diversas reacções ácido-base que podem afectar a absorção de fármacos. Ao longo do TGI, o grau de ionização é fundamental para a passagem através das membranas celulares, pois seja qual for a natureza dos fármacos, geralmente, a taxa de absorção por difusão passiva aumenta com um baixo grau de ionização, ou seja com o fármaco na forma não-ionizada. No entanto, para além do pH fisiológico, existem diversos factores, como a formulação do próprio fármaco, as interacções com outros medicamentos e com alimentos, que podem influenciar a dissolução, solubilidade e grau de ionização do fármaco. A indústria farmacêutica tem vindo a desenvolver estratégias e modelos in vitro, de modo a melhorar a formulação e certificar as propriedades dos fármacos que intervêm na sua absorção. Ao introduzir lauril sulfato de sódio (LSS), um surfactante, na fase externa da formulação do fármaco, aumenta a solubilidade e.g. do cetoprofeno, quer na forma ionizada ou não-ionizada. A adição de polímeros como o Kollicoat®SR 30D e o Kollicoat®MAE 30DP para aumentar a solubilidade e permeabilidade de fármacos quando a sua absorção fica comprometida com o valor de pH do meio e.g. do verapamilo. A associação dos modelos in vitro Caco-2, em que é usado a linhagem celular de adenocarcinoma do cólon humano, e o PAMPA (permeação em membrana artificial paralela) são ideais para a previsão da permeabilidade intestinal, permitindo priorizar as moléculas com propriedades mais favoráveis.

Advisors/Committee Members: Ferreira, Ana Paula.

Subjects/Keywords: Absorção; Ionização; TGI; In vitro

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APA (6^th Edition):

Calheiros, A. M. C. d. G. (2013). Relação ácido-base na absorção de fármacos. (Thesis). RCAAP. Retrieved from https://www.rcaap.pt/detail.jsp?id=oai:comum.rcaap.pt:10400.26/14121

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Calheiros, Ana Manuel Casanova de Girão. “Relação ácido-base na absorção de fármacos.” 2013. Thesis, RCAAP. Accessed January 21, 2021. https://www.rcaap.pt/detail.jsp?id=oai:comum.rcaap.pt:10400.26/14121.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Calheiros, Ana Manuel Casanova de Girão. “Relação ácido-base na absorção de fármacos.” 2013. Web. 21 Jan 2021.

Vancouver:

Calheiros AMCdG. Relação ácido-base na absorção de fármacos. [Internet] [Thesis]. RCAAP; 2013. [cited 2021 Jan 21]. Available from: https://www.rcaap.pt/detail.jsp?id=oai:comum.rcaap.pt:10400.26/14121.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Calheiros AMCdG. Relação ácido-base na absorção de fármacos. [Thesis]. RCAAP; 2013. Available from: https://www.rcaap.pt/detail.jsp?id=oai:comum.rcaap.pt:10400.26/14121

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

16. CARDOSO, Thalita Pedon de Araujo. Estratégias e implementação de técnicas in vitro, ex vivo e in vivo para avaliação da passagem dérmica e ocular de resíduos de pesticidas .

Degree: 2020, Universidade Federal de Pernambuco

URL: https://repositorio.ufpe.br/handle/123456789/37676

► Após um período de ênfase nas ações governamentais para a modernização da agricultura na região nordeste do Brasil, o Vale de São Francisco tornou-se um… (more)

▼ Após um período de ênfase nas ações governamentais para a modernização da agricultura na região nordeste do Brasil, o Vale de São Francisco tornou-se um local fértil e propício ao cultivo, principalmente de frutas, onde é possível encontrar grandes empresas e pequenos agricultores. Assim, o agrotóxico dimetoato (organofosforado) amplamente utilizado nesta região foi selecionado como objeto desde estudo. O estudo de caso in vivo foi realizado com três trabalhadores através de duas abordagens: 1) uma secção de algodão, imitando uma peça de roupa, fixada nas roupas do trabalhador, em diferentes regiões com área exposta específica para investigar a exposição dérmica do dimetoato; 2) a quantificação do estrato córneo foi realizada pela técnica de tape-striping para avaliar a absorção dérmica do dimetoato. Uma abordagem in vitro usando células Franz foi realizada aplicando-se dimetoato (0,4 e 1,8 μg) diretamente na pele de porco ou em uma seção de algodão antes de entrar em contato com a pele de porco. Dois estudos ex vivo foram ainda realizados em, um com córneas bovinas excisadas frescas e refrigerada e o outro com olhos bovinos inteiros frescos, refrigerados e danificados. Em ambos os experimentos foram aplicados 50 μL (5 mg/mL) de uma formulação de pesticida emulsificável (Dimetoato 500 EC®). Os resultados in vivo demonstraram os altos níveis de agrotóxicos aos quais os agricultores estão expostos, com a exposição dérmica potencial total entre 0,87-2,85 mg/pessoa/h e o fator estimado de penetração de EC(PF) entre 0-54,0 e 0- 28,9% para a nuca e os braços, respectivamente. Para o estudo in vitro, a quantidade de pesticida retido no EC foi de 52,63 ± 10,73 e 135,15 ± 31,8 ng/cm² após a aplicação diretamente no EC e demonstrando uma boa concordância com a abordagem in vivo. No estudo de absorção ocular ex vivo, os perfis de dimetoato, os fluxos e os coeficientes de permeação não apresentaram diferenças estatísticas (p <0,05) entre córneas excisadas frescas e refrigeradas. Em relação aos resultados obtidos para permeações em olhos inteiros, houve diferença estatística (p <0,05) entre os experimentos realizados com olhos frescos e os realizados com olhos refrigerados/danificados. Assim, os estudos preliminares demonstram que a abordagem ex vivo desenvolvida utilizando olhos inteiros é simples e demonstrou ser reprodutível para avaliação de agrotóxicos. É evidente que testes adicionais precisam ser realizados utilizando-se outros pesticidas com características físico-químicas distintas para uma melhor compreensão da passagem dos agrotóxicos pela pele. Advisors/Committee Members: SANTANA, Davi Pereira de (advisor), BEDOR, Cheila Nataly Galindo (advisor), LEAL, Leila Bastos (advisor), http://lattes.cnpq.br/1781541984014126 (advisor), http://lattes.cnpq.br/2164253878550042 (advisor), http://lattes.cnpq.br/9396720850659766 (advisor).

Subjects/Keywords: Agroquímicos; Técnicas in vitro; Pele

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

CARDOSO, T. P. d. A. (2020). Estratégias e implementação de técnicas in vitro, ex vivo e in vivo para avaliação da passagem dérmica e ocular de resíduos de pesticidas . (Doctoral Dissertation). Universidade Federal de Pernambuco. Retrieved from https://repositorio.ufpe.br/handle/123456789/37676

Chicago Manual of Style (16^th Edition):

CARDOSO, Thalita Pedon de Araujo. “Estratégias e implementação de técnicas in vitro, ex vivo e in vivo para avaliação da passagem dérmica e ocular de resíduos de pesticidas .” 2020. Doctoral Dissertation, Universidade Federal de Pernambuco. Accessed January 21, 2021. https://repositorio.ufpe.br/handle/123456789/37676.

MLA Handbook (7^th Edition):

CARDOSO, Thalita Pedon de Araujo. “Estratégias e implementação de técnicas in vitro, ex vivo e in vivo para avaliação da passagem dérmica e ocular de resíduos de pesticidas .” 2020. Web. 21 Jan 2021.

Vancouver:

CARDOSO TPdA. Estratégias e implementação de técnicas in vitro, ex vivo e in vivo para avaliação da passagem dérmica e ocular de resíduos de pesticidas . [Internet] [Doctoral dissertation]. Universidade Federal de Pernambuco; 2020. [cited 2021 Jan 21]. Available from: https://repositorio.ufpe.br/handle/123456789/37676.

Council of Science Editors:

CARDOSO TPdA. Estratégias e implementação de técnicas in vitro, ex vivo e in vivo para avaliação da passagem dérmica e ocular de resíduos de pesticidas . [Doctoral Dissertation]. Universidade Federal de Pernambuco; 2020. Available from: https://repositorio.ufpe.br/handle/123456789/37676

University of Toronto

17. Mosa, Adam Jonathan. Optimization of Organotypic Hippocampal Slice Cultures for Adult Neurogenesis Research: Exploring Pharmacological Interactions in Newborn Dentate Granule Cells.

Degree: 2014, University of Toronto

URL: http://hdl.handle.net/1807/68851

►

The organotypic hippocampal slice culture technique is an in vitro system for explanting the hippocampus and incubating tissue for up to four weeks. This method… (more)

Subjects/Keywords: in vitro; neurogenesis; organotypic; 0317

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APA (6^th Edition):

Mosa, A. J. (2014). Optimization of Organotypic Hippocampal Slice Cultures for Adult Neurogenesis Research: Exploring Pharmacological Interactions in Newborn Dentate Granule Cells. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/68851

Chicago Manual of Style (16^th Edition):

Mosa, Adam Jonathan. “Optimization of Organotypic Hippocampal Slice Cultures for Adult Neurogenesis Research: Exploring Pharmacological Interactions in Newborn Dentate Granule Cells.” 2014. Masters Thesis, University of Toronto. Accessed January 21, 2021. http://hdl.handle.net/1807/68851.

MLA Handbook (7^th Edition):

Mosa, Adam Jonathan. “Optimization of Organotypic Hippocampal Slice Cultures for Adult Neurogenesis Research: Exploring Pharmacological Interactions in Newborn Dentate Granule Cells.” 2014. Web. 21 Jan 2021.

Vancouver:

Mosa AJ. Optimization of Organotypic Hippocampal Slice Cultures for Adult Neurogenesis Research: Exploring Pharmacological Interactions in Newborn Dentate Granule Cells. [Internet] [Masters thesis]. University of Toronto; 2014. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/1807/68851.

Council of Science Editors:

Mosa AJ. Optimization of Organotypic Hippocampal Slice Cultures for Adult Neurogenesis Research: Exploring Pharmacological Interactions in Newborn Dentate Granule Cells. [Masters Thesis]. University of Toronto; 2014. Available from: http://hdl.handle.net/1807/68851

University of Debrecen

18. Kovács, Álmos. Vörös elemi nanoszelén és nátrium-szelenát biológiai hatásának összehasonlító vizsgálata in vitro dohánytenyészetben .

Degree: DE – ATC – Mezőgazdaság- Élelmiszertudományi és Környezetgazdálkodási Kar, 2011, University of Debrecen

URL: http://hdl.handle.net/2437/117878

► A szelén a legintenzívebben kutatott mikroelemek közé tartozik napjainkban. A nagy érdeklődés annak köszönhető, hogy bebizonyosodott esszenciális volta magassabbrendű állatokban, és az emberben is. Magasabbrendű… (more)

▼ A szelén a legintenzívebben kutatott mikroelemek közé tartozik napjainkban. A nagy érdeklődés annak köszönhető, hogy bebizonyosodott esszenciális volta magassabbrendű állatokban, és az emberben is. Magasabbrendű növényekben eddig nem bizonyították létfontosságú szerepét, bár pozitív hatását sok esetben tapasztalták alacsony koncentrációtartományban módosulatoktól függően. Növényekkel végzett kísérletekben a leggyakrabban használt szelénformák a szelenát illetve szelenit. E két forma fordul elő leggyakrabban a természetben és a növények számára a leginkább hozzáférhető módosulatok, ezzel magyarázható elterjedt alkalmazásuk. Van azonban egy érdekes, állatkísérletekben ígéretesnek tűnő szelénforma, a vörös elemi nanoszelén, melyet növényekben eddig még sohasem vizsgáltak. Dr. Prokisch József révén rendelkezésünkre állt tisztított nanoszelén szuszpenzió, így került kísérleteink homlokterébe. A kísérleteket dohány kallusztenyészetben és a mikrohajtásokból regenerálódó növényekben végeztem. Összehasonlításként a leggyakrabban használt szervetlen szelénformát, nátrium-szelenátot alkalmaztam. Eredményeink alapján megállapítható, hogy a vörös elemi nanoszelén partikulumok képesek bejutni a dohány in vitro szövettenyészetében, s ott a kalluszon át képződő mikrohajtások képződését stimulálta (különösen 50 - 100 mg/l koncentráció tartományban). A képződő mikrohajtások szabályos formájúak voltak normál szöveti szerkezettel, vitrifikáció megjelenése nélkül. Ez azért volt különösen szembetűnő, mivel a kontrol táptalajon a képződött mikrohajtások jelentős része abnormális alakú volt, áttetsző, vizenyős szöveti szerkezetük vitrifikációra utalt. Nanose-nel ellentétben szelenát esetében 10 mg/l koncentrációig indult meg a kalluszosodás, 50-100 mg/l tartomány azonban már gátolta a kalluszképződést. A nanoszelén hatását vizsgálva regenerálódó növényekben azt tapasztaltuk, hogy az főként a gyökérrészben akkumulálódott, ahol extrém magas értékeket tudtunk kimutatni. A hajtásrészben kis mértékben szállítódott fel, amit a transzport index számítása is alátámasztott. A nanoSe-nel szemben a szelenát forma főként hajtásrészben halmozódott fel, s a gyökérben csak kis mennyiség maradt. Az átoltott, regenerálódó hajtástenyészetekre is jellemző volt az a tendencia, amely a kalluszképződésnél is látható volt: e szerint ≥50 mg/l szelenát teljesen megakadályozta a gyökérképződést, a hajtások egy ideig vegetáltak, majd elpusztultak. Ezzel szemben a magas nanoSe tartalom (50-100 mg/l) kifejezetten segítette a gyökérképződés megindulását és a gyökerek frisstömeg gyarapodását is. Feltételezésünk szerint a szelenát és a nanoSe közötti biológiai hatásban tapasztalt különbség a két szelénforma kémiai természetéből adódhat. A szelenát, mint ionos forma bejutva a szövetekbe hamarabb okoz toxikus tüneteket. Ezt támasztotta alá a morfológiai vizsgálataink és a klorofilltartalom méréseink is. Az elemi nanoszelén azonban önmagában indifferens a növény számára. Vizes közegben azonban nagyon lassan ionok válhatnak le… Advisors/Committee Members: Fári, Miklós (advisor).

Subjects/Keywords: nanoszelén; in vitro dohány tenyészet

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kovács, �. (2011). Vörös elemi nanoszelén és nátrium-szelenát biológiai hatásának összehasonlító vizsgálata in vitro dohánytenyészetben . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/117878

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kovács, Álmos. “Vörös elemi nanoszelén és nátrium-szelenát biológiai hatásának összehasonlító vizsgálata in vitro dohánytenyészetben .” 2011. Thesis, University of Debrecen. Accessed January 21, 2021. http://hdl.handle.net/2437/117878.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kovács, Álmos. “Vörös elemi nanoszelén és nátrium-szelenát biológiai hatásának összehasonlító vizsgálata in vitro dohánytenyészetben .” 2011. Web. 21 Jan 2021.

Vancouver:

Kovács �. Vörös elemi nanoszelén és nátrium-szelenát biológiai hatásának összehasonlító vizsgálata in vitro dohánytenyészetben . [Internet] [Thesis]. University of Debrecen; 2011. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/2437/117878.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kovács �. Vörös elemi nanoszelén és nátrium-szelenát biológiai hatásának összehasonlító vizsgálata in vitro dohánytenyészetben . [Thesis]. University of Debrecen; 2011. Available from: http://hdl.handle.net/2437/117878

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Debrecen

19. Resetár, Anna. Alkaloid termelésre alkalmas Galanthus nivalis szövettenyészetek előállítása .

Degree: DE – TEK – Természettudományi és Technológiai Kar – Biológiai és Ökológiai Intézet, 2012, University of Debrecen

URL: http://hdl.handle.net/2437/128414

► A kikeleti hóvirág (Galanthus nivalis) az egyszikűek (Liliopsida) osztályának, a spárgavirágúak (Asparagales) rendjéhez, ezen belül az amarilliszfélék (Amaryllidaceae) családjához tartozó védett növényfaj. A Magyarországon élő… (more)

Subjects/Keywords: in vitro; Galanthus nivalis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Resetár, A. (2012). Alkaloid termelésre alkalmas Galanthus nivalis szövettenyészetek előállítása . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/128414

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Resetár, Anna. “Alkaloid termelésre alkalmas Galanthus nivalis szövettenyészetek előállítása .” 2012. Thesis, University of Debrecen. Accessed January 21, 2021. http://hdl.handle.net/2437/128414.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Resetár, Anna. “Alkaloid termelésre alkalmas Galanthus nivalis szövettenyészetek előállítása .” 2012. Web. 21 Jan 2021.

Vancouver:

Resetár A. Alkaloid termelésre alkalmas Galanthus nivalis szövettenyészetek előállítása . [Internet] [Thesis]. University of Debrecen; 2012. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/2437/128414.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Resetár A. Alkaloid termelésre alkalmas Galanthus nivalis szövettenyészetek előállítása . [Thesis]. University of Debrecen; 2012. Available from: http://hdl.handle.net/2437/128414

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Debrecen

20. Balázs, Bence. Védett növények in vitro a Debreceni Egyetem Növénytani Tanszékén .

Degree: DE – Természettudományi és Technológiai Kar – Biológiai és Ökológiai Intézet, 2014, University of Debrecen

URL: http://hdl.handle.net/2437/190851

Védett növények in vitro mikroszaporítása. A Debreceni Egyetem Növénytani Tanszékén található védett növények bemutatása. Advisors/Committee Members: Máthé, Csaba (advisor).

Subjects/Keywords: in vitro szövettenyésztés; mikroszaporítás

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Balázs, B. (2014). Védett növények in vitro a Debreceni Egyetem Növénytani Tanszékén . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/190851

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Balázs, Bence. “Védett növények in vitro a Debreceni Egyetem Növénytani Tanszékén .” 2014. Thesis, University of Debrecen. Accessed January 21, 2021. http://hdl.handle.net/2437/190851.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Balázs, Bence. “Védett növények in vitro a Debreceni Egyetem Növénytani Tanszékén .” 2014. Web. 21 Jan 2021.

Vancouver:

Balázs B. Védett növények in vitro a Debreceni Egyetem Növénytani Tanszékén . [Internet] [Thesis]. University of Debrecen; 2014. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/2437/190851.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Balázs B. Védett növények in vitro a Debreceni Egyetem Növénytani Tanszékén . [Thesis]. University of Debrecen; 2014. Available from: http://hdl.handle.net/2437/190851

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Debrecen

21. Krasznai, Levente. In vitro módszerek Aloe vera és fehér akác (Robinia pseudoacacia) esetében .

Degree: DE – TEK – Természettudományi és Technológiai Kar – Biológiai és Ökológiai Intézet, 2013, University of Debrecen

URL: http://hdl.handle.net/2437/170210

Dolgozatomban két, habitusában és ökológiai igényeiben is eltérő növény in vitro körülmények közötti alkalmazását mutatom be. Advisors/Committee Members: Mikóné Hamvas, Márta (advisor).

Subjects/Keywords: in vitro; mikroszaporítás; Robinia pseudoacacia

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APA (6^th Edition):

Krasznai, L. (2013). In vitro módszerek Aloe vera és fehér akác (Robinia pseudoacacia) esetében . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/170210

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Krasznai, Levente. “In vitro módszerek Aloe vera és fehér akác (Robinia pseudoacacia) esetében .” 2013. Thesis, University of Debrecen. Accessed January 21, 2021. http://hdl.handle.net/2437/170210.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Krasznai, Levente. “In vitro módszerek Aloe vera és fehér akác (Robinia pseudoacacia) esetében .” 2013. Web. 21 Jan 2021.

Vancouver:

Krasznai L. In vitro módszerek Aloe vera és fehér akác (Robinia pseudoacacia) esetében . [Internet] [Thesis]. University of Debrecen; 2013. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/2437/170210.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Krasznai L. In vitro módszerek Aloe vera és fehér akác (Robinia pseudoacacia) esetében . [Thesis]. University of Debrecen; 2013. Available from: http://hdl.handle.net/2437/170210

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

22. Modin, Anna. En beskrivning av hur copingstrategier används av personer som har eller ska genomgå In Vitro Fertiliseringsbehandling, utifrån Lazarus och Folkmans teorier om coping : En kvalitativ studie.

Degree: Health and Caring Sciences, 2011, University of Gävle

URL: http://urn.kb.se/resolve?urn=urn:nbn:se:hig:diva-11193

► Syfte: Syftet med denna studie var att beskriva hur Lazarus och Folkmans copingstrategier används av kvinnor och män som genomgår IVF behandling, vid första… (more)

Subjects/Keywords: coping; In Vitro fertilisering; infertilitet

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APA (6^th Edition):

Modin, A. (2011). En beskrivning av hur copingstrategier används av personer som har eller ska genomgå In Vitro Fertiliseringsbehandling, utifrån Lazarus och Folkmans teorier om coping : En kvalitativ studie. (Thesis). University of Gävle. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:hig:diva-11193

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Modin, Anna. “En beskrivning av hur copingstrategier används av personer som har eller ska genomgå In Vitro Fertiliseringsbehandling, utifrån Lazarus och Folkmans teorier om coping : En kvalitativ studie.” 2011. Thesis, University of Gävle. Accessed January 21, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:hig:diva-11193.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Modin, Anna. “En beskrivning av hur copingstrategier används av personer som har eller ska genomgå In Vitro Fertiliseringsbehandling, utifrån Lazarus och Folkmans teorier om coping : En kvalitativ studie.” 2011. Web. 21 Jan 2021.

Vancouver:

Modin A. En beskrivning av hur copingstrategier används av personer som har eller ska genomgå In Vitro Fertiliseringsbehandling, utifrån Lazarus och Folkmans teorier om coping : En kvalitativ studie. [Internet] [Thesis]. University of Gävle; 2011. [cited 2021 Jan 21]. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:hig:diva-11193.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Modin A. En beskrivning av hur copingstrategier används av personer som har eller ska genomgå In Vitro Fertiliseringsbehandling, utifrån Lazarus och Folkmans teorier om coping : En kvalitativ studie. [Thesis]. University of Gävle; 2011. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:hig:diva-11193

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Delft University of Technology

23. Langeslag, S.A.E. (author). Maturation Enhancement of Cardiac Myocytes In Vitro: Development of a Loading Protocol.

Degree: 2011, Delft University of Technology

URL: http://resolver.tudelft.nl/uuid:31a47ae2-d68f-44cc-aeea-eede570702c1

► One of the major challenges in drug development is the development of a drug-screening model that closely resembles the adult human heart. Currently many drugs… (more)

▼ One of the major challenges in drug development is the development of a drug-screening model that closely resembles the adult human heart. Currently many drugs are rejected in late stages of development and even withdrawn from the market due to possible cardiac tissue damaging side effects. The corresponding delay in drug development is mainly due to a lack in drug-screening methods, to determine these life-threatening side effects. Cultivation of human cardiac cells in vitro could provide such a drug-screening model. Human cardiac muscle cells can be derived from human embryonic stem cells. However, the cardiac muscle cells derived by culturing these human embryonic stem cells into cardiomyocytes (cardiac muscle cells) appear to be immature in relation to cardiac muscle cells taken directly from the adult human body. This immaturity results in a drug-screening model with limited predictability. The hypothesis of the Cytostretch project, a collaborative project between Leiden University Medical Centre and Philips Research, is that in vitro cultured cardiomyocytes subjected to in vivo mimicking mechanical stimuli (stretch), will show enhanced maturation. These mature cell cultures provide a good basis for future drug-screening models. A chip is developed containing a polydimethylsiloxane (PDMS) thin-film as a substrate for cardiomyocyte culturing. A pressure difference is applied to the membrane to obtain a strained membrane, which with anchored cardiomyocytes will result in cardiomyocyte stretch. In order to stretch cardiac myocytes in vitro, the development of an in vivo mimicking loading protocol is essential. The main goal for the presented study, therefore, is the development of a proper loading protocol for in vitro stretching of cardiomyocytes. To reach this goal two study objectives have been defined; investigation of in vivo cardiac muscle strain during a normal cardiac cycle and the determination of the PDMS thin-film behavior. For the determination of the in vivo cardiac muscle strain, the left ventricle was modelled mathematically. Assumed was a homogeneous stress distribution along the left ventricular wall, corresponding to a fiber direction course along the wall, consistent with anatomical findings. The relation between left ventricular fiber stress and left ventricle pressure showed to depend mainly on the ratio of cavity volume over wall volume. The left ventricle mechanics can be approximated by: Where ?f is the left ventricular fiber stress, Plv the left ventricular pressure. Vlv is the left ventricular cavity volume, Vw the left ventricular wall volume and ??f the natural fiber strain. The outcome of the mathematical left ventricle model led to the conclusion that the absolute left ventricular fiber strain between end systole (reference volume) and end diastole equals an approximate 14.7% for a healthy adult human heart during normal cardiac cycle. The PDMS thin-film behavior is modelled analytically with use of classical thin plate mechanics, considering large deformations. The analytical derived… Advisors/Committee Members: Van Keulen, A. (mentor).

Subjects/Keywords: Cardiac Myocytes; In Vitro

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APA (6^th Edition):

Langeslag, S. A. E. (. (2011). Maturation Enhancement of Cardiac Myocytes In Vitro: Development of a Loading Protocol. (Masters Thesis). Delft University of Technology. Retrieved from http://resolver.tudelft.nl/uuid:31a47ae2-d68f-44cc-aeea-eede570702c1

Chicago Manual of Style (16^th Edition):

Langeslag, S A E (author). “Maturation Enhancement of Cardiac Myocytes In Vitro: Development of a Loading Protocol.” 2011. Masters Thesis, Delft University of Technology. Accessed January 21, 2021. http://resolver.tudelft.nl/uuid:31a47ae2-d68f-44cc-aeea-eede570702c1.

MLA Handbook (7^th Edition):

Langeslag, S A E (author). “Maturation Enhancement of Cardiac Myocytes In Vitro: Development of a Loading Protocol.” 2011. Web. 21 Jan 2021.

Vancouver:

Langeslag SAE(. Maturation Enhancement of Cardiac Myocytes In Vitro: Development of a Loading Protocol. [Internet] [Masters thesis]. Delft University of Technology; 2011. [cited 2021 Jan 21]. Available from: http://resolver.tudelft.nl/uuid:31a47ae2-d68f-44cc-aeea-eede570702c1.

Council of Science Editors:

Langeslag SAE(. Maturation Enhancement of Cardiac Myocytes In Vitro: Development of a Loading Protocol. [Masters Thesis]. Delft University of Technology; 2011. Available from: http://resolver.tudelft.nl/uuid:31a47ae2-d68f-44cc-aeea-eede570702c1

University of Minnesota

24. Stading, Amy. Development and Applications of In Vitro-Microdialysis: A Sampling Platform for Fast Analysis of Non-Electroactive Analytes.

Degree: PhD, Chemistry, 2016, University of Minnesota

URL: http://hdl.handle.net/11299/181667

► When considering the measurement of release events from cells, it can be done at levels as small as the single cell and performed in systems… (more)

▼ When considering the measurement of release events from cells, it can be done at levels as small as the single cell and performed in systems increasingly larger and more complex up to in vivo studies. Though in vitro systems lack the physiological relevance of in vivo, their simple and controlled environment is highly advantageous in preliminary mechanistic studies. In spite of this, there exists a serious gap in our ability to perform in vitro measurements on a wide array of analytes within a meaningful time frame. While electrochemical techniques are unparalleled in their ability to temporally resolve minute signals in biological systems, there is only a small class of targets which are suitable for this type of analysis. When analyzing non-electroactive analytes, measurements are often plagued by slow temporal responses (5+ minutes). Fluorescent imaging offers opportunities to monitor faster dynamics of non-electroactive analytes, but the target analyte must be either natively fluorescent or labeled, which can result in nonspecific binding and cytotoxicity. In both of these cases fast dynamics can be observed, but the array of analytes is small and only a few can be monitored simultaneously. In this work, a novel in vitro sampling platform is described which is capable of simultaneously monitoring approximately 15 non-electroactive analytes with 20 second temporal resolution. Cells were cultured on the surface of a microdialysis probe coupled with an analytical system for analysis. Small molecules released from the cells upon stimulation diffuse across the porous membrane because of the close proximity. A high-speed CE, built in house, enabled analysis of the collected dialysate. The ability of our platform to detect basal and stimulated release of amines was confirmed by transferring the probe between artificial cerebrospinal fluid (aCSF) and a potassium-spiked (100 mM K+-aCSF) stimulant solution. A variety of cell models were tested for compatibility with the in vitro-microdialysis platform, both single cell type and co-cultures were initiated. Adherence of viable cells was confirmed by labeling cells with either fluorescein diacetate (FDA) or specific antibody labelling, followed by imaging under a microscope. As a step towards continuously monitoring the change of non-electroactive analytes released from cultured cells, microdialysis was coupled directly to micro free flow electrophoresis (µFFE) device instead of the high-speed CE instrument.

Subjects/Keywords: Capillary Electrophoresis; In Vitro; Microdialysis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Stading, A. (2016). Development and Applications of In Vitro-Microdialysis: A Sampling Platform for Fast Analysis of Non-Electroactive Analytes. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/181667

Chicago Manual of Style (16^th Edition):

Stading, Amy. “Development and Applications of In Vitro-Microdialysis: A Sampling Platform for Fast Analysis of Non-Electroactive Analytes.” 2016. Doctoral Dissertation, University of Minnesota. Accessed January 21, 2021. http://hdl.handle.net/11299/181667.

MLA Handbook (7^th Edition):

Stading, Amy. “Development and Applications of In Vitro-Microdialysis: A Sampling Platform for Fast Analysis of Non-Electroactive Analytes.” 2016. Web. 21 Jan 2021.

Vancouver:

Stading A. Development and Applications of In Vitro-Microdialysis: A Sampling Platform for Fast Analysis of Non-Electroactive Analytes. [Internet] [Doctoral dissertation]. University of Minnesota; 2016. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/11299/181667.

Council of Science Editors:

Stading A. Development and Applications of In Vitro-Microdialysis: A Sampling Platform for Fast Analysis of Non-Electroactive Analytes. [Doctoral Dissertation]. University of Minnesota; 2016. Available from: http://hdl.handle.net/11299/181667

Universitat de Valencia

25. Escrich Albelda, Laura. Maduración in vitro de ovocitos humanos desestimados de ciclos de estimulación ovárica .

Degree: 2015, Universitat de Valencia

URL: http://hdl.handle.net/10550/50735

► El éxito de las técnicas de reproducción asistida está directamente relacionado con el número y grado de madurez de los ovocitos recuperados tras la punción… (more)

▼ El éxito de las técnicas de reproducción asistida está directamente relacionado con el número y grado de madurez de los ovocitos recuperados tras la punción folicular, siendo únicamente útiles reproductivamente, aquéllos maduros en estadio de MII; existiendo un 11% en estadio inmaduro (VG) y, por tanto, no útiles reproductivamente. Por ello, planteamos el rescate de ovocitos inmaduros en estadio VG como estrategia reproductiva a fin de aumentar el número de ovocitos iniciales. El objetivo de esta tesis es optimizar las tasas de maduración in vitro (MIV) a nivel nuclear, citoplasmático y genómico de los ovocitos humanos en estadio de VG, recuperados en ciclos de estimulación ovárica controlada con indicación de ICSI. Para ello se han realizado 3 estudios. En el primero, se evaluó la correlación de ciertos parámetros morfométricos y morfológicos no invasivos con la condensación y distribución de la cromatina, en relación al nucléolo. Tras analizar 131 VG, éstas se agruparon retrospectivamente en 3 modelos y las variables morfométricas y morfológicas comparadas. Tras agrupar las VG en función de la condensación de la cromatina, se observó que las variables: diámetro ovocitario, continuidad de la envoltura nuclear, posición del nucléolo y apariencia del nucleoplasma permitieron predecir, mediante un modelo matemático, el grado de condensación de la cromatina, manteniendo la viabilidad del ovocito. En el segundo estudio, se evaluó la maduración ovocitaria in vitro y su correlación con el grado de condensación de la cromatina. Para ello, 131 VG fueron cultivadas en un sistema convencional (SC) y, el grado de maduración nuclear alcanzado evaluado a intervalos regulares de tiempo. Tras 24h, la tasa de maduración nuclear, o capacidad de los ovocitos VG para progresar in vitro a MII, fue del 55%, aumentando al 75% tras prolongar el cultivo hasta 42h. Respecto a la competencia citoplasmática, o capacidad de los ovocitos MII de responder a un procedimiento de activación ovocitaria artificial, un 64% de los ovocitos MIV activó, porcentaje significativamente inferior al observado en ovocitos control (100%). Igualmente, la tasa de activación normal, fue significativamente inferior en el grupo de MIV (22.4% vs. 79.2%). Respeto a la competencia genómica o determinación de la ploidía por FISH observamos que todos los ovocitos con respuesta normal fueron haploides. Finalmente, tras aplicar el modelo matemático predictivo del grado de condensación de la cromatina, observamos que esta variable no muestra relación alguna con la competencia nuclear, citoplasmática o genómica de los ovocitos VG, posiblemente debido a la homogeneidad de la población, en términos de condensación de la cromatina. En el tercero estudio, se describió la cinética de la maduración nuclear in vitro y su correlación con la competencia citoplasmática. Las VG cultivadas en sistema time-lapse (STL) mostraron una tasa de maduración nuclear superior a la obtenida tras cultivo en SC (88.9% vs. 74.8%); además, su competencia citoplasmática fue también superior (STL:… Advisors/Committee Members: Escribá Pérez, Mª José (advisor).

Subjects/Keywords: Maduración in vitro; Ovocito

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APA (6^th Edition):

Escrich Albelda, L. (2015). Maduración in vitro de ovocitos humanos desestimados de ciclos de estimulación ovárica . (Doctoral Dissertation). Universitat de Valencia. Retrieved from http://hdl.handle.net/10550/50735

Chicago Manual of Style (16^th Edition):

Escrich Albelda, Laura. “Maduración in vitro de ovocitos humanos desestimados de ciclos de estimulación ovárica .” 2015. Doctoral Dissertation, Universitat de Valencia. Accessed January 21, 2021. http://hdl.handle.net/10550/50735.

MLA Handbook (7^th Edition):

Escrich Albelda, Laura. “Maduración in vitro de ovocitos humanos desestimados de ciclos de estimulación ovárica .” 2015. Web. 21 Jan 2021.

Vancouver:

Escrich Albelda L. Maduración in vitro de ovocitos humanos desestimados de ciclos de estimulación ovárica . [Internet] [Doctoral dissertation]. Universitat de Valencia; 2015. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/10550/50735.

Council of Science Editors:

Escrich Albelda L. Maduración in vitro de ovocitos humanos desestimados de ciclos de estimulación ovárica . [Doctoral Dissertation]. Universitat de Valencia; 2015. Available from: http://hdl.handle.net/10550/50735

Universitat de Valencia

26. Fernández-Blanco Gómez, Celia. Estudios In Vitro de los mecanismos de toxicidad de las micotoxinas .

Degree: 2017, Universitat de Valencia

URL: http://hdl.handle.net/10550/58372

► Se ha llevado a cabo la evaluación in vitro de los efectos producidos por micotoxinas de Alternaria y Fusarium en células de mamífero. Se ha… (more)

▼ Se ha llevado a cabo la evaluación in vitro de los efectos producidos por micotoxinas de Alternaria y Fusarium en células de mamífero. Se ha evaluado la citotoxicidad individual del alternariol (AOH), alternariol monometil éter (AME), beauvericina (BEA), deoxinivalenol (DON), eniatina B (ENN B), fumonisina B1 (FB1), zearalenona (ZEA) y α-zearalenol (α-ZOL) en células de adenocarcinoma de colon humano (Caco-2) donde únicamente se obtuvieron valores de IC50 para la ENN B, DON, BEA y α- ZOL. La evaluación de la citotoxicidad combinada entre mezclas de micotoxinas en células Caco-2 mostró efecto sinérgico en las combinaciones de AOH+AME, DON+AOH y ENN B+AOH; efecto aditivo en la combinación DON+ENN B y efecto antagonista en la combinación terciaria DON+AOH+ENN B. Se estudió la bioaccesibilidad y biodisponibilidad del AOH, ZEA y α-ZOL. Se evaluó la bioaccesibilidad mediante el método de digestión estático in vitro, siendo el α-ZOL más bioaccesible a nivel gástrico y duodenal que la ZEA. Se observó una baja biodisponibilidad en las tres micotoxinas ensayadas con las células Caco-2/TC7, siendo el AOH el más biodisponible. Teniendo en cuenta la biodisponibilidad de las micotoxinas y los escasos estudios de mecanismos de toxicidad conocidos, se estudió la interacción de las micotoxinas con los componentes y la alteración de actividades celulares. Los resultados obtenidos demostraron que el AOH bloquea el ciclo celular en la fase G2/M, causa pérdida del potencial de la membrana mitocondrial y produce apoptosis a través de la vía mitocondrial. Además, se evaluó el daño causado por el AOH a nivel del ADN mediante el ensayo del cometa y se observó un incremento del daño dependiente de la concentración. Debido a la biodisponibilidad de las micotoxinas, se determinó la actividad estrogénica de algunas muy prevalentes como la FB1 y la BEA, observándose que la BEA produce mayor actividad estrogénica sobre células que la FB1. Dado que un mecanismo de citotoxicidad es el estrés oxidativo, se determina la capacidad del AOH para generar especies reactivas de oxígeno (ROS), evidenciándose que el AOH en las células Caco-2 produce ROS inmediatamente tras la exposición en todas lasconcentraciones ensayadas. Una de las consecuencias de las ROS es la oxidación de los lípidos de las membranas celulares, es decir, la generación de peroxidación lipídica (LPO). Los resultados obtenidos demostraron que el AOH aumenta significativamente la producción de LPO. Tras los resultados obtenidos se procedió a determinar la eficacia del sistema de defensa intracelular (enzimático y no enzimático) frente al estrés oxidativo. Estos indicaron un incremento de la actividad de la superóxido dismutasa (SOD) a todas las concentraciones de AOH expuestas en las células Caco-2 y que la actividad enzimática de la catalasa (CAT) fue más eficaz que la glutatión peroxidasa (GPx), eliminando peróxido de hidrógeno. Además, se demostró que el glutatión (GSH) y las enzimas implicadas en el … Advisors/Committee Members: Ruiz Leal, María José (advisor).

Subjects/Keywords: Micotoxinas; in vitro; Toxicología

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APA (6^th Edition):

Fernández-Blanco Gómez, C. (2017). Estudios In Vitro de los mecanismos de toxicidad de las micotoxinas . (Doctoral Dissertation). Universitat de Valencia. Retrieved from http://hdl.handle.net/10550/58372

Chicago Manual of Style (16^th Edition):

Fernández-Blanco Gómez, Celia. “Estudios In Vitro de los mecanismos de toxicidad de las micotoxinas .” 2017. Doctoral Dissertation, Universitat de Valencia. Accessed January 21, 2021. http://hdl.handle.net/10550/58372.

MLA Handbook (7^th Edition):

Fernández-Blanco Gómez, Celia. “Estudios In Vitro de los mecanismos de toxicidad de las micotoxinas .” 2017. Web. 21 Jan 2021.

Vancouver:

Fernández-Blanco Gómez C. Estudios In Vitro de los mecanismos de toxicidad de las micotoxinas . [Internet] [Doctoral dissertation]. Universitat de Valencia; 2017. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/10550/58372.

Council of Science Editors:

Fernández-Blanco Gómez C. Estudios In Vitro de los mecanismos de toxicidad de las micotoxinas . [Doctoral Dissertation]. Universitat de Valencia; 2017. Available from: http://hdl.handle.net/10550/58372

University of Limerick

27. Johnson, Gillian P. MSC contributions to loading-induced bone formation: a role for adenylyl cyclase 6.

Degree: 2019, University of Limerick

URL: http://hdl.handle.net/10344/8159

►

peer-reviewed

Physical loading is a potent regulator of bone anabolism, yet the cellular mechanisms by which this occurs are poorly understood. Due to the finite… (more)

▼

peer-reviewed

Physical loading is a potent regulator of bone anabolism, yet the cellular mechanisms by which this occurs are poorly understood. Due to the finite lifespan of bone forming osteoblasts, it is hypothesized that these cells must be replenished from a bone marrow stem cell (MSC) population in response to loading, as is seen in response to injury. It is hypothesized that the application of a mechanical stimulus may directly stimulate MSCs within the marrow to undergo osteogenic differentiation, as has been shown in vitro. However, how MSCs may sense this loading or whether loading actually regulates MSC differentiation in vivo is unknown. In this thesis, we first demonstrate that MSCs utilize cAMP as a 2nd messenger in mechanotransduction, that is required for flow mediated increases in osteogenic gene expression. Furthermore, we demonstrate that this mechanosignalling is dependent on the primary cilium and the ciliary localised adenylyl cyclase 6. Lastly, in this initial study we also demonstrate that this mechanotransduction mechanism can be targeted therapeutically to enhance cAMP concentrations and early osteogenic signalling, mimicking the beneficial effect of physical loading. In the second study of this thesis, we demonstrate that Gpr161 is a mechanoresponsive G-Protein Couple Receptor (GPCR), that localises to the cilium, and is required for fluid shear induced increases in cAMP and osteogenesis. This Gpr161 mediated mechanotransduction is dependent on the primary cilium but acts upstream of cilia localised adenylyl cyclase 6 (AC6), suggesting that Gpr161 may act through AC6 to regulate cAMP and MSC osteogenesis. Moreover, we demonstrate that Hedgehog (Hh) signalling is positively correlated with osteogenesis and demonstrate that Hh signalling is mechanically regulated and required for loading-induced MSC osteogenesis through a primary cilium-Gpr161-AC6-cAMP mechanism. Taking the first two studies together, we have delineated a molecular mechanism of MSC mechanotransduction which occurs at the primary cilium and can be targeted therapeutically, demonstrating a potential mechanotherapeutic for bone loss diseases such as osteoporosis. Finally, we utilized Leptin Receptor to identify and trace the contribution of bone marrow stem cells to bone mechanoadaptation in vivo. Lepr-Cre; tdTomato+ mice were subjected to compressive tibia loading and histological analysis revealed that Lepr-Cre; tdTomato+ stem cells arise perinatally around blood vessels and increase within the marrow following tibial compressive loading. Mechanical loading induces an increase in bone formation parameters yet loading does not result in an increase in Lepr-Cre; tdTomato+ osteoblasts or osteocytes. Moreover, mice with a LepR specific deletion of AC6 have an attenuated response to compressive tibia loading, nor is there a change in the percentage of Lepr-Cre; tdTomato+ cells. This therefore demonstrates that LepR+ cells on the bone surface directly contribute to bone formation and that adenylyl cyclase 6 is required for…

Advisors/Committee Members: Fair, Seán, Hoey, David A..

Subjects/Keywords: bone anabolism; in vitro

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APA (6^th Edition):

Johnson, G. P. (2019). MSC contributions to loading-induced bone formation: a role for adenylyl cyclase 6. (Thesis). University of Limerick. Retrieved from http://hdl.handle.net/10344/8159

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Johnson, Gillian P. “MSC contributions to loading-induced bone formation: a role for adenylyl cyclase 6.” 2019. Thesis, University of Limerick. Accessed January 21, 2021. http://hdl.handle.net/10344/8159.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Johnson, Gillian P. “MSC contributions to loading-induced bone formation: a role for adenylyl cyclase 6.” 2019. Web. 21 Jan 2021.

Vancouver:

Johnson GP. MSC contributions to loading-induced bone formation: a role for adenylyl cyclase 6. [Internet] [Thesis]. University of Limerick; 2019. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/10344/8159.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Johnson GP. MSC contributions to loading-induced bone formation: a role for adenylyl cyclase 6. [Thesis]. University of Limerick; 2019. Available from: http://hdl.handle.net/10344/8159

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

28. Delplace, Céline. Microparticules à libération contrôlée : nouveaux polymères et importance des conditions de libération : Controlled release microparticles : novel polymers and insight into the importance of the release set-up.

Degree: Docteur es, Sciences du médicament, 2012, Université Lille II – Droit et Santé

URL: http://www.theses.fr/2012LIL2S007

► Les microparticules à base de copolymères d’acides lactique et glycolique (PLGA) sont biocompatibles et biodégradables tout en permettant de contrôler la libération des principes actifs… (more)

▼ Les microparticules à base de copolymères d’acides lactique et glycolique (PLGA) sont biocompatibles et biodégradables tout en permettant de contrôler la libération des principes actifs pendant quelques jours à plusieurs mois. Récemment, des stratégies ont été développées pour améliorer les propriétés de ces polymères en introduisant des groupements fonctionnels le long de la chaîne polymérique dans le but de moduler la libération des principes actifs.L’un des objectifs de ce travail a été d’étudier le potentiel de nouveaux copolymères fonctionnalisés portant des groupements carboxyliques pour la préparation de systèmes à libération contrôlée. L’apomorphine est encapsulée comme principe actif modèle. Son effet thérapeutique reste limité de part son court temps de demi-vie et son puissant effet émétique. Ainsi, des microparticules biodégradables, assurant une libération contrôlée de l’apomorphine, amélioreraient son efficacité thérapeutique et l’observance du traitement en réduisant la fréquence d’administration et les effets secondaires systémiques. Les microparticules chargées en apomorphine ont été préparées par une méthode d’émulsion à partir des nouveaux polymères fonctionnalisés, et de PLGA 50:50 de différentes masses moléculaires, pour comparaison. Les microparticules obtenues ont été caractérisées par différentes techniques. Le contenu résiduel en dichlorométhane (utilisé au cours de la formulation) a été quantifié et la libération de l’apomorphine a été étudiée in vitro. L’utilisation des polymères fonctionnalisés portant des fonctions carboxyliques libres a mené à une efficacité d’encapsulation plus élevée en apomorphine, de plus bas taux résiduels en dichlorométhane et à des cinétiques de libération in vitro de l’apomorphine distinctes de celles obtenues avec les PLGA traditionnels. Ces résultats suggèrent une application prometteuse de ces polymères fonctionnalisés pour la libération contrôlée de principes actifs. Une étape d’optimisation a ensuite consisté à modifier les paramètres de formulation afin d’étudier leur influence sur les caractéristiques des microparticules produites. L’objectif était notamment d’améliorer l’efficacité d’encapsulation tout en limitant la libération initiale d’apomorphine pouvant engendrer des pics de concentration néfastes, à l’origine d’effets indésirables. Ainsi, certains paramètres de formulation ont été modulés au cours de la préparation des microparticules à base de PLGA 50:50 de 10 kDa. La sélection de paramètres optimaux a mené au développement d’une formulation assurant une libération d’ordre zéro de l’apomorphine sur une période de dix jours.En outre, la littérature met en évidence l’importance des études de libération in vitro au cours du développement des microparticules de PLGA. Cependant, aucune méthode n’étant décrite par les autorités réglementaires, des conditions très différentes sont utilisées en pratique et leur influence sur les cinétiques de libération est peu connue. Par conséquent, une partie de ce travail a consisté à évaluer l’impact des conditions… Advisors/Committee Members: Siepmann, Juergen (thesis director), Siepmann, Florence (thesis director).

Subjects/Keywords: Microparticules; Essais in vitro

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APA (6^th Edition):

Delplace, C. (2012). Microparticules à libération contrôlée : nouveaux polymères et importance des conditions de libération : Controlled release microparticles : novel polymers and insight into the importance of the release set-up. (Doctoral Dissertation). Université Lille II – Droit et Santé. Retrieved from http://www.theses.fr/2012LIL2S007

Chicago Manual of Style (16^th Edition):

Delplace, Céline. “Microparticules à libération contrôlée : nouveaux polymères et importance des conditions de libération : Controlled release microparticles : novel polymers and insight into the importance of the release set-up.” 2012. Doctoral Dissertation, Université Lille II – Droit et Santé. Accessed January 21, 2021. http://www.theses.fr/2012LIL2S007.

MLA Handbook (7^th Edition):

Delplace, Céline. “Microparticules à libération contrôlée : nouveaux polymères et importance des conditions de libération : Controlled release microparticles : novel polymers and insight into the importance of the release set-up.” 2012. Web. 21 Jan 2021.

Vancouver:

Delplace C. Microparticules à libération contrôlée : nouveaux polymères et importance des conditions de libération : Controlled release microparticles : novel polymers and insight into the importance of the release set-up. [Internet] [Doctoral dissertation]. Université Lille II – Droit et Santé 2012. [cited 2021 Jan 21]. Available from: http://www.theses.fr/2012LIL2S007.

Council of Science Editors:

Delplace C. Microparticules à libération contrôlée : nouveaux polymères et importance des conditions de libération : Controlled release microparticles : novel polymers and insight into the importance of the release set-up. [Doctoral Dissertation]. Université Lille II – Droit et Santé 2012. Available from: http://www.theses.fr/2012LIL2S007

Virginia Tech

29. Vu, Lucas Trung. Proteomic Analysis of Three Dimensional Organotypic Liver Models.

Degree: PhD, Chemical Engineering, 2015, Virginia Tech

URL: http://hdl.handle.net/10919/77033

► In vitro liver models that closely mimic the in vivo microenvironment are central for understanding hepatic functions and intercellular communication processes. Bottom-up shotgun proteomic analysis… (more)

▼ In vitro liver models that closely mimic the in vivo microenvironment are central for understanding hepatic functions and intercellular communication processes. Bottom-up shotgun proteomic analysis of the hepatic cells can lend insight into such processes. This technique employs liquid chromatography-tandem mass spectrometry (LC-MS/MS) for relative quantification of protein abundances by measuring intensities of their corresponding peptides. Organotypic 3D liver models have been developed in our laboratory that consist of hepatocytes and liver sinusoidal endothelial cells (LSECs) separated by a polyelectrolyte multilayer (PEM), which serves as a mimic for the Space of Disse. Each component within these models is easily separable allowing for systematic evaluation of the cells and PEMs. In this study, proteomes of hepatocytes from PEM containing models, cultured with and without LSECs, were compared to those from monolayers. Changes in core metabolism were evaluated among all culture conditions. Overall, all cultures were ketogenic and performed gluconeogenesis. The presence of the PEM led to increases in proteins associated with mitochondrial-based β-oxidation and peroxisomal proteins. The PEMs also limited production of structural proteins, which are linked to dedifferentiation of hepatocytes, suggesting that cell-ECM interactions are essential for maintenance of their liver-like state. The presence of LSECs increased levels of carboxylesterases and other phase I and phase II detoxification enzymes suggesting that intercellular signaling mediates enzyme abundance. Taken together, these results suggest that the cell-cell (from the LSECs) and cell-ECM (from the PEMs) interactions exert different, yet crucial effects, and both are required for the preservation of metabolic liver functions and differentiated phenotypes. Changes in the PEMs as a result of cell culture were also evaluated but exhibited minimal differences at this time point. Proteomes of LSECs monolayers were also characterized. Enzymes related to the metabolism of amino acids, lipids, oxidative phosphorylation and phase I and phase II detoxification processes were all identified in LSECs monolayers highlighting their role in these processes. Characterization of 3DHL LSECs was not possible due to ion suppression resulting from the presence of excess contaminant proteins. Nonetheless, this study provides a foundation in which LSECs from 3D liver models can be compared against in future studies. Advisors/Committee Members: Rajagopalan, Padmavathy (committeechair), Helm, Richard F. (committeechair), Murali, T. M. (committee member), Lu, Chang (committee member), Davis, Richey M. (committee member).

Subjects/Keywords: Shotgun Proteomics; Liver; In vitro

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APA (6^th Edition):

Vu, L. T. (2015). Proteomic Analysis of Three Dimensional Organotypic Liver Models. (Doctoral Dissertation). Virginia Tech. Retrieved from http://hdl.handle.net/10919/77033

Chicago Manual of Style (16^th Edition):

Vu, Lucas Trung. “Proteomic Analysis of Three Dimensional Organotypic Liver Models.” 2015. Doctoral Dissertation, Virginia Tech. Accessed January 21, 2021. http://hdl.handle.net/10919/77033.

MLA Handbook (7^th Edition):

Vu, Lucas Trung. “Proteomic Analysis of Three Dimensional Organotypic Liver Models.” 2015. Web. 21 Jan 2021.

Vancouver:

Vu LT. Proteomic Analysis of Three Dimensional Organotypic Liver Models. [Internet] [Doctoral dissertation]. Virginia Tech; 2015. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/10919/77033.

Council of Science Editors:

Vu LT. Proteomic Analysis of Three Dimensional Organotypic Liver Models. [Doctoral Dissertation]. Virginia Tech; 2015. Available from: http://hdl.handle.net/10919/77033

University of Guelph

30. Nicholson, James. Improving the Ex Vitro Acclimation Efficiency of Hybrid Hazelnuts (Corylus avellana L. X C. americana Marshall).

Degree: MS, Department of Plant Agriculture, 2019, University of Guelph

URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/17398

► Micropropagation of hybrid hazelnuts (Corylus avellana L. X C. americana Marshall) commercially has been limited due to their poor rooting ability plus low survival and… (more)

Subjects/Keywords: micropropagation; hazelnuts; hybrid hazelnuts; acclimation; ex vitro; Corylus avellana; in vitro; in vitro rooting

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APA (6^th Edition):

Nicholson, J. (2019). Improving the Ex Vitro Acclimation Efficiency of Hybrid Hazelnuts (Corylus avellana L. X C. americana Marshall). (Masters Thesis). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/17398

Chicago Manual of Style (16^th Edition):

Nicholson, James. “Improving the Ex Vitro Acclimation Efficiency of Hybrid Hazelnuts (Corylus avellana L. X C. americana Marshall).” 2019. Masters Thesis, University of Guelph. Accessed January 21, 2021. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/17398.

MLA Handbook (7^th Edition):

Nicholson, James. “Improving the Ex Vitro Acclimation Efficiency of Hybrid Hazelnuts (Corylus avellana L. X C. americana Marshall).” 2019. Web. 21 Jan 2021.

Vancouver:

Nicholson J. Improving the Ex Vitro Acclimation Efficiency of Hybrid Hazelnuts (Corylus avellana L. X C. americana Marshall). [Internet] [Masters thesis]. University of Guelph; 2019. [cited 2021 Jan 21]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/17398.

Council of Science Editors:

Nicholson J. Improving the Ex Vitro Acclimation Efficiency of Hybrid Hazelnuts (Corylus avellana L. X C. americana Marshall). [Masters Thesis]. University of Guelph; 2019. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/17398

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