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Universidade Estadual de Campinas

1. Menezes, Cláudia Beatriz Afonso de, 1977-. Taxonomia polifásica com ênfase em Multilocus Sequence Analysis (MLSA) e bioprospecção de compostos bioativos de actinomicetos isolados de ambiente marinho: Polyfhasic taxonomy emphazing Multilocus Sequence Analysis (MLSA) and bioprospecting of bioactive compounds from actinomycetes isolated from marine environment.

Degree: 2015, Universidade Estadual de Campinas

Abstract: O ambiente marinho representa uma importante fonte de diversidade biológica com grande potencial para descoberta de novos metabólitos secundários biologicamente ativos. Dentro desse ambiente, pode-se destacar os actinomicetos marinhos, cujos estudos podem contribuir para a melhor compreensão de suas funções ecológicas e para seu uso como uma fonte importante de novos metabólitos. A taxonomia dos actinomicetos é bastante complexa e a metodologia de MLSA, ferramenta alternativa em estudos de sistemática microbiana, pode ser uma técnica importante na caracterização de actinomicetos. Novos metabólitos secundários tem sido isolados de actinomicetos marinhos, sendo as atividades antibacteriana e anticâncer as principais descritas,porém pouco se conhece sobre a atividade antiviral deste grupo de micro-organimos. Neste contexto, o presente trabalho teve por objetivo avaliar a diversidade de actinomicetos associados ao ambiente marinho, utilizando uma abordagem polifásica, associada à avaliação das atividades antimicrobiana e antiviral destas bactérias. No total, foram obtidos 579 isolados de bactérias oriundos de macro-organismos marinhos coletados no litoral norte do Estado de São Paulo, Brasil. Desses, 72 foram identificados por análise de sequência do gene RNA ribossomal 16S, como actinomicetos, e nove isolados foram descritos como novas espécies de actinobactérias pertencentes aos gêneros Gordonia (B204), Marmoricola (B374), Williamsia (B138, B375 e B452), Serinicoccus (B736), Kineococcus (B366), Knoellia (B175) e Janibacter (B742). A caracterização polifásica dessas novas espécies de actinobactérias foi realizada pela técnica de hibridação DNA-DNA, Multilocus Sequence Analysis (MLSA) utilizando genes conservados (rpoB, rpoA, gyrB, recA e trpB), análise de perfil de ácidos graxos e microscopia eletrônica. A caracterização dos isolados foi complementada por testes fisiológicos e quimiotaxonômicos, tais como a determinação do conteúdo de GC, lipídios polares, menaquinonas, testes de utilização de fontes de carbono, atividade enzimática, degradação de compostos e tolerância a antibióticos, pH e temperatura. Quanto ao potencial biotecnológico dos 72 isolados de actinobactérias avaliados, 16 isolados apresentaram potencial antimicrobiano e 13 potencial antiviral. Os extratos dos isolados B175 (CIM = 1 mg/mL), B375 (CIM = 2 mg/mL), B138 (CIM = 1 mg/mL) e B366 (CIM = 2 mg/mL) apresentaram atividade antimicrobiana frente a Staphylococcus aureus ATCC 6538. O extrato do isolado B374 apresentou atividade antiviral frente ao vírus Metapneumovírus aviário (aMPV), os isolados B366 e B742, frente ao herpes vírus simplex do tipo 1 (HSV-1), os isolados B138 e B452 frente ao vírus Calicivírus Felino (FCV) e o isolado B204 frente ao vírus da diarréia viral bovina (BVDV). A partir deste trabalho, pode-se concluir que existe grande diversidade de micro-organismos marinhos a ser descoberta e explorada, como fonte de novas espécies e compostos com potencial biotecnológico Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Fantinatti-Garboggini, Fabiana, 1965- (advisor), Universidade Estadual de Campinas. Instituto de Biologia (institution), Programa de Pós-Graduação em Genética e Biologia Molecular (nameofprogram), Ruiz, Ana Lucia Tasca Gois (committee member), Destéfano, Suzete Aparecida Lanza (committee member), Gonçalves, Edmilson Ricardo (committee member), Vasconcellos, Suzan Pantaroto de (committee member).

Subjects/Keywords: Actinobactéria - Classificação; Agentes antivirais; Atividade antimicrobiana; Actinobacteria - Classification; Antiviral agent; Antimicrobial activity

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APA (6th Edition):

Menezes, Cláudia Beatriz Afonso de, 1. (2015). Taxonomia polifásica com ênfase em Multilocus Sequence Analysis (MLSA) e bioprospecção de compostos bioativos de actinomicetos isolados de ambiente marinho: Polyfhasic taxonomy emphazing Multilocus Sequence Analysis (MLSA) and bioprospecting of bioactive compounds from actinomycetes isolated from marine environment. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/316710

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Menezes, Cláudia Beatriz Afonso de, 1977-. “Taxonomia polifásica com ênfase em Multilocus Sequence Analysis (MLSA) e bioprospecção de compostos bioativos de actinomicetos isolados de ambiente marinho: Polyfhasic taxonomy emphazing Multilocus Sequence Analysis (MLSA) and bioprospecting of bioactive compounds from actinomycetes isolated from marine environment.” 2015. Thesis, Universidade Estadual de Campinas. Accessed October 31, 2020. http://repositorio.unicamp.br/jspui/handle/REPOSIP/316710.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Menezes, Cláudia Beatriz Afonso de, 1977-. “Taxonomia polifásica com ênfase em Multilocus Sequence Analysis (MLSA) e bioprospecção de compostos bioativos de actinomicetos isolados de ambiente marinho: Polyfhasic taxonomy emphazing Multilocus Sequence Analysis (MLSA) and bioprospecting of bioactive compounds from actinomycetes isolated from marine environment.” 2015. Web. 31 Oct 2020.

Vancouver:

Menezes, Cláudia Beatriz Afonso de 1. Taxonomia polifásica com ênfase em Multilocus Sequence Analysis (MLSA) e bioprospecção de compostos bioativos de actinomicetos isolados de ambiente marinho: Polyfhasic taxonomy emphazing Multilocus Sequence Analysis (MLSA) and bioprospecting of bioactive compounds from actinomycetes isolated from marine environment. [Internet] [Thesis]. Universidade Estadual de Campinas; 2015. [cited 2020 Oct 31]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/316710.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Menezes, Cláudia Beatriz Afonso de 1. Taxonomia polifásica com ênfase em Multilocus Sequence Analysis (MLSA) e bioprospecção de compostos bioativos de actinomicetos isolados de ambiente marinho: Polyfhasic taxonomy emphazing Multilocus Sequence Analysis (MLSA) and bioprospecting of bioactive compounds from actinomycetes isolated from marine environment. [Thesis]. Universidade Estadual de Campinas; 2015. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/316710

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. Ber, Polona. Aeromicrobium choanae sp. nov., nova vrsta aktinobakterije, izolirana iz sapišča vrtne penice.

Degree: 2017, Univerza v Mariboru

V okviru preiskovanja bakterijske mikrobiote sapišč izbranih vrst ptic, je bil v predhodnih raziskavah iz sapišča vrtne penice (Sylvia borin) izoliran sev 9H-4 (Matjašič, 2016). Na osnovi delne sekvence gena za 16S rRNA je bil sev uvrščen v rod Aeromicrobium. Z namenom, da določimo njegov natančen taksonomski položaj, smo ga v pričujoči magistrski nalogi nadalje okarakterizirali z različnimi fenotipskimi in genotipskimi pristopi. Analiza celotnega nukleotidnega zaporedja genov za 16S rRNA je pokazala, da sev 9H-4 spada v rod Aeromicrobium z najbližjima sorodnima vrstama Aeromicrobium flavum in Aeromicrobium tamlense. Na osnovi sekvenc genov za 16S rRNA smo med vrstami iz rodu Aeromicrobium izračunali filogenetske odnose z metodo združevanja sosedov in konstruirali filogenetsko drevo. Sev 9H-4 v filogenetskem drevesu oblikuje samostojno vejo, dobro ločeno od najbolj sorodnih vrst. S preiskovanjem fenotipskih lastnosti seva 9H-4 smo ugotovili, da se ta sev od vrst A. flavum in A. tamlense razlikuje v sposobnosti hidrolize kazeina in pozitivni aktivnosti encimov β-galaktozidaze ter valin arilamidaze. S tankoplastno kromatografijo smo preiskali hidrolizat celične stene seva 9H-4 in ugotovili, da je sestavina peptidoglikana LL-diaminopimelična kislina. Nadaljnja primerjava genoma seva 9H-4 z genomoma referenčnih sevov Aeromicrobium flavum DSM 19355T in Aeromicrobium tamlense DSM 19087T na osnovi DNA-DNA hibridizacije, ki je bila narejena v sodelovanju z raziskovalno skupino iz zbirke mikroorganizmov BCCM/LMG v Belgiji, je odkrila manj kot 70% podobnosti z obema vrstama. Slednja analiza je potrdila, da Aeromicrobium sp. 9H-4 predstavlja novo vrsto bakterije. Sev 9H-4T smo opisali kot tipski sev za novo vrsto Aeromicrobium choanae (Ber in sod., 2017).

In the frame of studying the microbial diversity from the choana of selected bird species, strain 9H-4 was isolated in previous research work (Matjašič, 2016) from the choana of the garden warbler (Sylvia borin). Based on the partial sequence of the 16S rRNA gene, the strain was classified into the genus Aeromicrobium. In order to determine its precise taxonomic position, the strain was in the present master's thesis characterized more in details using various phenotypic and genotypic approaches. An analysis of the entire nucleotide sequence of the 16S rRNA genes has shown that the strain 9H-4 belongs to the genus Aeromicrobium with the closest related species Aeromicrobium flavum and Aeromicrobium tamlense. On the basis of the 16S rRNA gene sequence, the phylogenetic relationship between the species of the genus Aeromicrobium was calculated using the neighbour-joining method and a phylogenetic tree was constructed. Strain 9H-4 forms in the phylogenetic tree an independent branch, well separated from the most closely related species. By further analysis of the phenotypic characteristics of strain 9H-4, we discovered that the strain differentiates from its closest relatives A. flavum and A. tamlense in its capability to hydrolyse casein and positive enzyme activities of…

Advisors/Committee Members: Trček, Janja.

Subjects/Keywords: aktinobakterije; rod Aeromicrobium; Aeromicrobium sp. nov.; actinobacteria; Aeromicrobium; Aeromicrobium sp. nov.; info:eu-repo/classification/udc/579(043.2)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Ber, P. (2017). Aeromicrobium choanae sp. nov., nova vrsta aktinobakterije, izolirana iz sapišča vrtne penice. (Masters Thesis). Univerza v Mariboru. Retrieved from https://dk.um.si/IzpisGradiva.php?id=68631 ; https://dk.um.si/Dokument.php?id=119262&dn= ; https://plus.si.cobiss.net/opac7/bib/23423496?lang=sl

Chicago Manual of Style (16th Edition):

Ber, Polona. “Aeromicrobium choanae sp. nov., nova vrsta aktinobakterije, izolirana iz sapišča vrtne penice.” 2017. Masters Thesis, Univerza v Mariboru. Accessed October 31, 2020. https://dk.um.si/IzpisGradiva.php?id=68631 ; https://dk.um.si/Dokument.php?id=119262&dn= ; https://plus.si.cobiss.net/opac7/bib/23423496?lang=sl.

MLA Handbook (7th Edition):

Ber, Polona. “Aeromicrobium choanae sp. nov., nova vrsta aktinobakterije, izolirana iz sapišča vrtne penice.” 2017. Web. 31 Oct 2020.

Vancouver:

Ber P. Aeromicrobium choanae sp. nov., nova vrsta aktinobakterije, izolirana iz sapišča vrtne penice. [Internet] [Masters thesis]. Univerza v Mariboru; 2017. [cited 2020 Oct 31]. Available from: https://dk.um.si/IzpisGradiva.php?id=68631 ; https://dk.um.si/Dokument.php?id=119262&dn= ; https://plus.si.cobiss.net/opac7/bib/23423496?lang=sl.

Council of Science Editors:

Ber P. Aeromicrobium choanae sp. nov., nova vrsta aktinobakterije, izolirana iz sapišča vrtne penice. [Masters Thesis]. Univerza v Mariboru; 2017. Available from: https://dk.um.si/IzpisGradiva.php?id=68631 ; https://dk.um.si/Dokument.php?id=119262&dn= ; https://plus.si.cobiss.net/opac7/bib/23423496?lang=sl

3. Haesly, Doran John. Taxonomic Analysis of Marine Actinomycetic Isolates.

Degree: 1959, North Texas State College

Though this current study was initiated independently and was not a test laboratory for the taxonomic sub-committee's evaluative program, the problem outlined in this treatise was also designed in an effort to test certain characteristics of the actinomycetes of both a biochemical and morphological nature. This problem employed methods that might absolve or establish certain criteria for taxonomic use in the group of actinomycetes. Advisors/Committee Members: Roach, Archibald W., Guthrie, Rufus K..

Subjects/Keywords: taxonomic analysis; marine actinomycetic isolates; Actinobacteria  – Classification.; Marine bacteria  – Classification.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Haesly, D. J. (1959). Taxonomic Analysis of Marine Actinomycetic Isolates. (Thesis). North Texas State College. Retrieved from https://digital.library.unt.edu/ark:/67531/metadc108052/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Haesly, Doran John. “Taxonomic Analysis of Marine Actinomycetic Isolates.” 1959. Thesis, North Texas State College. Accessed October 31, 2020. https://digital.library.unt.edu/ark:/67531/metadc108052/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Haesly, Doran John. “Taxonomic Analysis of Marine Actinomycetic Isolates.” 1959. Web. 31 Oct 2020.

Vancouver:

Haesly DJ. Taxonomic Analysis of Marine Actinomycetic Isolates. [Internet] [Thesis]. North Texas State College; 1959. [cited 2020 Oct 31]. Available from: https://digital.library.unt.edu/ark:/67531/metadc108052/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Haesly DJ. Taxonomic Analysis of Marine Actinomycetic Isolates. [Thesis]. North Texas State College; 1959. Available from: https://digital.library.unt.edu/ark:/67531/metadc108052/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.