subject:(ATP)

University of Waikato

1. Cumming, Mathew Hoani. Structural and enzymatic characterisation of nucleoside triphosphate diphosphohydrolases from Trifolium repens and Dolichos biflorus .

Degree: 2012, University of Waikato

URL: http://hdl.handle.net/10289/6331

► Extracellular nucleoside triphosphate diphosphohydrolases (NTPDases) are enzymes that reduce the extracellular nucleotide signal and inactivate the purinogenic signalling pathway. These enzymes, in the presence of… (more)

▼ Extracellular nucleoside triphosphate diphosphohydrolases (NTPDases) are enzymes that reduce the extracellular nucleotide signal and inactivate the purinogenic signalling pathway. These enzymes, in the presence of a divalent cation sequentially hydrolyses the γ- and β- phosphoanhydride bond from a range of nucleotide di- or triphosphates to the corresponding nucleotide monophosphate. There is evidence that purinogenic signalling is present in higher plants and it is becoming clear that NTPDases play a role in the early stages of rhizobium infection during nodulation in legumes. To further our understanding of NTPDases, this study investigated the biochemical and structural characteristics of two legume NTPDases believed to be involved in nodulation. The first, 7WC, was isolated from the roots of white clover and the second, DbLnP, from the roots of Dolichos biflorus. DbLnP has been characterised as a carbohydrate binding NTPDase that is directly associated with the perception of rhizobia. Using X-ray crystallography a number of crystal structures of 7WC and DbLnP were determined at resolutions between 1.9 Å and 2.9 Å. For 7WC, structures were determined for an apo- form, an AMP-bound and also bound with the nonhydrolysable ATP analogue AMPPNP. For DbLnP structures were solved with phosphate and Mn2+ bound and another with AMPPNP and Mn2+ bound. Kinetic analysis of a range of substrates together with the analysis of the binding modes of 7WC and DbLnP explained substrate preference for each of the NTPDases. These analyses showed that NTPDases can adopt two conformations depending on substrate and co-factor binding. The central hinge region creates a ‘butterfly’ motion of the domains that reduces the width of the active site cleft. This phenomenon has been previously hypothesised but has not been observed for NTPDases. A model of catalysis is proposed whereby the ‘open’ form first binds substrate in an inactive orientation. Binding of the metal ion induces a conformational change that brings the domains together and allows movement of the phosphate tail deeper into the active site cleft – a reorganisation that is required for catalysis. Hydrolysis occurs via nucleophilic attack on the terminal phosphate. Finally, release of the metal ion allows the ‘open’ conformation to be restored for subsequent catalysis to occur. Advisors/Committee Members: Arcus, Vickery L (advisor), Roberts, Nick (advisor).

Subjects/Keywords: NTPDase; ATP

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APA (6^th Edition):

Cumming, M. H. (2012). Structural and enzymatic characterisation of nucleoside triphosphate diphosphohydrolases from Trifolium repens and Dolichos biflorus . (Doctoral Dissertation). University of Waikato. Retrieved from http://hdl.handle.net/10289/6331

Chicago Manual of Style (16^th Edition):

Cumming, Mathew Hoani. “Structural and enzymatic characterisation of nucleoside triphosphate diphosphohydrolases from Trifolium repens and Dolichos biflorus .” 2012. Doctoral Dissertation, University of Waikato. Accessed March 07, 2021. http://hdl.handle.net/10289/6331.

MLA Handbook (7^th Edition):

Cumming, Mathew Hoani. “Structural and enzymatic characterisation of nucleoside triphosphate diphosphohydrolases from Trifolium repens and Dolichos biflorus .” 2012. Web. 07 Mar 2021.

Vancouver:

Cumming MH. Structural and enzymatic characterisation of nucleoside triphosphate diphosphohydrolases from Trifolium repens and Dolichos biflorus . [Internet] [Doctoral dissertation]. University of Waikato; 2012. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10289/6331.

Council of Science Editors:

Cumming MH. Structural and enzymatic characterisation of nucleoside triphosphate diphosphohydrolases from Trifolium repens and Dolichos biflorus . [Doctoral Dissertation]. University of Waikato; 2012. Available from: http://hdl.handle.net/10289/6331

Universidad de Cantabria

2. Fidalgo Gómez, Estefanía. Intracellular ATP production by CD4 T cells: comparison of different conditions of cells sources and clinical utility in renal transplantation.

Degree: Máster en Biología Molecular y Biomedicina, 2013, Universidad de Cantabria

URL: http://hdl.handle.net/10902/4193

► The ImmuKnow® uses complete fresh blood to measure the intracellular ATP production. The technique cannot be doing in samples older than 30 hours after the… (more)

Subjects/Keywords: Producción de ATP

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APA (6^th Edition):

Fidalgo Gómez, E. (2013). Intracellular ATP production by CD4 T cells: comparison of different conditions of cells sources and clinical utility in renal transplantation. (Masters Thesis). Universidad de Cantabria. Retrieved from http://hdl.handle.net/10902/4193

Chicago Manual of Style (16^th Edition):

Fidalgo Gómez, Estefanía. “Intracellular ATP production by CD4 T cells: comparison of different conditions of cells sources and clinical utility in renal transplantation.” 2013. Masters Thesis, Universidad de Cantabria. Accessed March 07, 2021. http://hdl.handle.net/10902/4193.

MLA Handbook (7^th Edition):

Fidalgo Gómez, Estefanía. “Intracellular ATP production by CD4 T cells: comparison of different conditions of cells sources and clinical utility in renal transplantation.” 2013. Web. 07 Mar 2021.

Vancouver:

Fidalgo Gómez E. Intracellular ATP production by CD4 T cells: comparison of different conditions of cells sources and clinical utility in renal transplantation. [Internet] [Masters thesis]. Universidad de Cantabria; 2013. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10902/4193.

Council of Science Editors:

Fidalgo Gómez E. Intracellular ATP production by CD4 T cells: comparison of different conditions of cells sources and clinical utility in renal transplantation. [Masters Thesis]. Universidad de Cantabria; 2013. Available from: http://hdl.handle.net/10902/4193

Arizona State University

3. Yang, Jay-How. Isolation and Functional Studies of The F-type ATP Synthase from Spinach Chloroplasts and Heliobacterium modesticaldum.

Degree: Biochemistry, 2015, Arizona State University

URL: http://repository.asu.edu/items/29992

► Adenosine triphosphate (ATP) is the universal chemical energy currency in most living cells, used to power many cellular reactions and generated by an enzyme supercomplex… (more)

▼ Adenosine triphosphate (ATP) is the universal chemical energy currency in most living cells, used to power many cellular reactions and generated by an enzyme supercomplex known as the ATP synthase, consisting of a hydrophilic F1 subcomplex and a membrane-bound FO subcomplex. Driven by the electrochemical gradient generated by the respiratory or photosynthetic electron transport chain, the rotation of the FO domain drives movements of the central stalk in response to conformational changes in the F1 domain, in which the physical energy is converted into chemical energy through the condensation of ADP and Pi to ATP. The exact mechanism how ATP synthesis is coupled to proton translocation is not known as no structure of the intact ATP-synthase nor the intact FO subcomplex has been determined to date. Structural information may shed light on these mechanisms and aid in understanding how structural changed relate to its coupling to ATP synthesis. The work in this thesis has successful established a defined large-scale CF1FO isolation procedure resulting in high purity and high yield of this complex from spinach thylakoid membranes by incorporating a unique combination of biochemical methods will form the basis for the subsequent structural determination of this complex. Isolation began from the isolation of intact chloroplasts and the separation of intact thylakoid membranes. Both native and denaturing electrophoresis analyses clearly demonstrated that the purified CF1FO retains its quaternary structure consisting of the CF1 and CFO subcomplexes and nine subunits (five F1 subunits: α, β, γ, δ and ε, and four FO subunits: a, b, b' and c). Moreover, both ATP synthesis and hydrolysis activities were successfully detected using protein reconstitution in combination with acid-base incubation and in-gel ATPase assays, respectively. Furthermore, the ATP-synthase of H. modesticaldum, an anaerobic photosynthetic bacterium, was also isolated and characterized at the biochemical level. These biochemical characterizations directly influenced recent studies on the high-resolution structure determination of intact CF1FO using electron crystallography on two-dimensional crystals. The availability of the functionally intact CF1FO purified at a large scale will lead to studies that investigate the possible crystallization conditions to ultimately determine its three-dimensional structure at atomic resolution.

Subjects/Keywords: Biochemistry; ATP synthase

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APA (6^th Edition):

Yang, J. (2015). Isolation and Functional Studies of The F-type ATP Synthase from Spinach Chloroplasts and Heliobacterium modesticaldum. (Doctoral Dissertation). Arizona State University. Retrieved from http://repository.asu.edu/items/29992

Chicago Manual of Style (16^th Edition):

Yang, Jay-How. “Isolation and Functional Studies of The F-type ATP Synthase from Spinach Chloroplasts and Heliobacterium modesticaldum.” 2015. Doctoral Dissertation, Arizona State University. Accessed March 07, 2021. http://repository.asu.edu/items/29992.

MLA Handbook (7^th Edition):

Yang, Jay-How. “Isolation and Functional Studies of The F-type ATP Synthase from Spinach Chloroplasts and Heliobacterium modesticaldum.” 2015. Web. 07 Mar 2021.

Vancouver:

Yang J. Isolation and Functional Studies of The F-type ATP Synthase from Spinach Chloroplasts and Heliobacterium modesticaldum. [Internet] [Doctoral dissertation]. Arizona State University; 2015. [cited 2021 Mar 07]. Available from: http://repository.asu.edu/items/29992.

Council of Science Editors:

Yang J. Isolation and Functional Studies of The F-type ATP Synthase from Spinach Chloroplasts and Heliobacterium modesticaldum. [Doctoral Dissertation]. Arizona State University; 2015. Available from: http://repository.asu.edu/items/29992

Ruhr Universität Bochum

4. Veitinger, Thomas. Physiologische Untersuchungen von Signalwegen in reifen und unreifen Keimzellen männlicher Säugetiere.

Degree: 2009, Ruhr Universität Bochum

URL: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-30273

► In der vorliegenden Arbeit wurden physiologische Reaktionen von menschlichen Spermien im Zusammenhang mit Chemotaxis und Motilität untersucht. Dabei wurden Spermien verschiedenen Duftstoffen ausgesetzt und mit… (more)

Subjects/Keywords: Spermium; Calcium; ATP; Spermatogenese; Chemotaxis

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APA (6^th Edition):

Veitinger, T. (2009). Physiologische Untersuchungen von Signalwegen in reifen und unreifen Keimzellen männlicher Säugetiere. (Thesis). Ruhr Universität Bochum. Retrieved from http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-30273

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Veitinger, Thomas. “Physiologische Untersuchungen von Signalwegen in reifen und unreifen Keimzellen männlicher Säugetiere.” 2009. Thesis, Ruhr Universität Bochum. Accessed March 07, 2021. http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-30273.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Veitinger, Thomas. “Physiologische Untersuchungen von Signalwegen in reifen und unreifen Keimzellen männlicher Säugetiere.” 2009. Web. 07 Mar 2021.

Vancouver:

Veitinger T. Physiologische Untersuchungen von Signalwegen in reifen und unreifen Keimzellen männlicher Säugetiere. [Internet] [Thesis]. Ruhr Universität Bochum; 2009. [cited 2021 Mar 07]. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-30273.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Veitinger T. Physiologische Untersuchungen von Signalwegen in reifen und unreifen Keimzellen männlicher Säugetiere. [Thesis]. Ruhr Universität Bochum; 2009. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-30273

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Alberta

5. Lai, Aaron. Determining factors in the differential activation of microglia.

Degree: PhD, Centre for Neuroscience, 2010, University of Alberta

URL: https://era.library.ualberta.ca/files/tb09j6275

► Microglia, the resident immune cells of the central nervous system (CNS), become activated in response to danger signals given out by other cells when homeostasis… (more)

▼ Microglia, the resident immune cells of the central nervous system (CNS), become activated in response to danger signals given out by other cells when homeostasis has been disturbed. Microglial activation is a multifaceted phenomenon that includes numerous distinct phenotypes. The type of activation often influences the survival of surrounding CNS tissue, and thus gaining a better understanding of how microglial activation is regulated has important therapeutic implications. Currently, it is known that the phenotype of activated microglia depends on both the type of CNS insult and the specific activating agent. The aim of this thesis was to investigate the potential involvement of other determining factors. Extrinsic regulators of microglial activation, including the severity of CNS insult and the stimulation strength of activating agents, were examined. Intrinsic differences among different microglial populations, namely differences in region of origin and age of origin, were also investigated. To study microglial behavior without interference from other cells, rat primary cultures were used as the system of study. With regard to extrinsic factors, it was found that different severities of hypoxic neuronal injury induced distinct microglial phenotypes. Among the activating agents released by injured neurons, adenosine 5’-triphosphate (ATP) was studied in isolation and was found to induce trophic and toxic effectors in microglia depending on the strength of ATP stimulation. In regards to intrinsic factors, it was found that microglia derived from different regions of the brain had distinct responses to activators, with cortical and hippocampal microglia generating more toxic responses than brainstem, striatal, and thalamic microglia. Microglia derived from various ages of origin also responded differentially to activators, with neonatal and aged microglia being more reactive than microglia derived from other age groups. Together, the results here present several novel concepts, that the phenotype of activated microglia are dependent not only on the type of activating stimulus, but the strength of that stimulus, and that in addition to stimuli from other cells, the regional and age differences among microglia themselves are also crucial in determining their activation phenotype.

Subjects/Keywords: ATP; hypoxia; neuroinflammation; neuroscience; microglia

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APA (6^th Edition):

Lai, A. (2010). Determining factors in the differential activation of microglia. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/tb09j6275

Chicago Manual of Style (16^th Edition):

Lai, Aaron. “Determining factors in the differential activation of microglia.” 2010. Doctoral Dissertation, University of Alberta. Accessed March 07, 2021. https://era.library.ualberta.ca/files/tb09j6275.

MLA Handbook (7^th Edition):

Lai, Aaron. “Determining factors in the differential activation of microglia.” 2010. Web. 07 Mar 2021.

Vancouver:

Lai A. Determining factors in the differential activation of microglia. [Internet] [Doctoral dissertation]. University of Alberta; 2010. [cited 2021 Mar 07]. Available from: https://era.library.ualberta.ca/files/tb09j6275.

Council of Science Editors:

Lai A. Determining factors in the differential activation of microglia. [Doctoral Dissertation]. University of Alberta; 2010. Available from: https://era.library.ualberta.ca/files/tb09j6275

University of Alberta

6. Zwicker, Jennifer D. ATP and central respiratory control: a three-part signaling system.

Degree: PhD, Department of Physiology, 2012, University of Alberta

URL: https://era.library.ualberta.ca/files/ws859g94q

► ATP actions on central inspiratory networks are determined by a three-part signaling system comprising: i) excitatory actions of ATP at P2 receptors (Rs) ii) ectonucleotidases… (more)

▼ ATP actions on central inspiratory networks are determined by a three-part signaling system comprising: i) excitatory actions of ATP at P2 receptors (Rs) ii) ectonucleotidases that degrade ATP into adenosine (ADO), and iii) inhibitory actions of ADO at P1Rs. During hypoxia, an initial increase in ventilation is followed by a secondary depression that is life threatening in premature infants. The release of ATP in respiratory networks, including the preBötzinger Complex (preBötC, inspiratory rhythm generation site) attenuates this secondary ventilatory depression. However, subsequent degradation of ATP to ADO may exacerbate the depression. The objective of my thesis research is to explore the significance of this three-part signaling system for preBötC networks in rodents using rhythmically active medullary slices from rats and mice, primary cultures of preBötC glia, and anesthetized adult rats. In neonatal rats in vitro, injection of ATP into the preBötC evokes an increase in inspiratory breathing frequency via a P2Y1R mechanism that involves both neurons and glia. Analysis of cultured preBötC glia suggests that P2Y1R stimulation evokes an increase in Ca2+ and release of glutamate, which excites inspiratory neurons. In contrast, injection of ATP into the preBötC of rhythmic slices from neonatal mice evokes a P2Y1R-mediated frequency increase if A1 ADORs are blocked. In contrast to rats, neonatal mice are sensitive to ADO inhibition of preBötC frequency and have higher expression of the ectonucleotidase TNAP (an enzyme that degrades ATP to ADO). A delicate balance between P2R actions and P1R actions modulates the preBötC network of neonatal rodents. Purinergic signaling also influences the activity of mature preBötC networks in adult rats. Injection of a P2Y1R agonist into the preBötC evoked a 40% increase in respiratory frequency while ADO injection had no effect. As predicted based on the proposed role of ATP in attenuating the secondary hypoxic ventilatory depression, the increase of endogenous ectonucleotidase activity in the preBötC (via injection of a lentivirus controlling expression of the enzyme TMPAP) produced a greater secondary hypoxic ventilatory depression compared to control. This work lays the foundation for future research examining the importance of glia and purinergic modulation within the rhythmogenic inspiratory network.

Subjects/Keywords: ATP; PreBotzinger Complex; Glia; Adenosine

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APA (6^th Edition):

Zwicker, J. D. (2012). ATP and central respiratory control: a three-part signaling system. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/ws859g94q

Chicago Manual of Style (16^th Edition):

Zwicker, Jennifer D. “ATP and central respiratory control: a three-part signaling system.” 2012. Doctoral Dissertation, University of Alberta. Accessed March 07, 2021. https://era.library.ualberta.ca/files/ws859g94q.

MLA Handbook (7^th Edition):

Zwicker, Jennifer D. “ATP and central respiratory control: a three-part signaling system.” 2012. Web. 07 Mar 2021.

Vancouver:

Zwicker JD. ATP and central respiratory control: a three-part signaling system. [Internet] [Doctoral dissertation]. University of Alberta; 2012. [cited 2021 Mar 07]. Available from: https://era.library.ualberta.ca/files/ws859g94q.

Council of Science Editors:

Zwicker JD. ATP and central respiratory control: a three-part signaling system. [Doctoral Dissertation]. University of Alberta; 2012. Available from: https://era.library.ualberta.ca/files/ws859g94q

Addis Ababa University

7. Asmamaw, Asrat. DYNAMICS OF MOTOR PROTEINS= A MONTE CARLO SIMULATION .

Degree: 2012, Addis Ababa University

URL: http://etd.aau.edu.et/dspace/handle/123456789/1190

► Motor proteins are mechanochemical enzymes that convert energy released by adenosine triphosphate (ATP) hydrolysis into either linear or rotary movement. Motor proteins: kinesin, myosin and… (more)

Subjects/Keywords: MOTOR PROTEINS; adenosine triphosphate (ATP)

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APA (6^th Edition):

Asmamaw, A. (2012). DYNAMICS OF MOTOR PROTEINS= A MONTE CARLO SIMULATION . (Thesis). Addis Ababa University. Retrieved from http://etd.aau.edu.et/dspace/handle/123456789/1190

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Asmamaw, Asrat. “DYNAMICS OF MOTOR PROTEINS= A MONTE CARLO SIMULATION .” 2012. Thesis, Addis Ababa University. Accessed March 07, 2021. http://etd.aau.edu.et/dspace/handle/123456789/1190.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Asmamaw, Asrat. “DYNAMICS OF MOTOR PROTEINS= A MONTE CARLO SIMULATION .” 2012. Web. 07 Mar 2021.

Vancouver:

Asmamaw A. DYNAMICS OF MOTOR PROTEINS= A MONTE CARLO SIMULATION . [Internet] [Thesis]. Addis Ababa University; 2012. [cited 2021 Mar 07]. Available from: http://etd.aau.edu.et/dspace/handle/123456789/1190.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Asmamaw A. DYNAMICS OF MOTOR PROTEINS= A MONTE CARLO SIMULATION . [Thesis]. Addis Ababa University; 2012. Available from: http://etd.aau.edu.et/dspace/handle/123456789/1190

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

8. Cline, Paul. Female Airline Transport Pilots: The Role of Mentoring.

Degree: PhD, Aviation, 2017, University of North Dakota

URL: https://commons.und.edu/theses/344

► Women have been a part of aviation since its inception, yet they have been traditionally underrepresented in the ranks of commercial pilots. This study… (more)

Subjects/Keywords: Airlines; ATP; Female; Mentoring; Pilot

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APA (6^th Edition):

Cline, P. (2017). Female Airline Transport Pilots: The Role of Mentoring. (Doctoral Dissertation). University of North Dakota. Retrieved from https://commons.und.edu/theses/344

Chicago Manual of Style (16^th Edition):

Cline, Paul. “Female Airline Transport Pilots: The Role of Mentoring.” 2017. Doctoral Dissertation, University of North Dakota. Accessed March 07, 2021. https://commons.und.edu/theses/344.

MLA Handbook (7^th Edition):

Cline, Paul. “Female Airline Transport Pilots: The Role of Mentoring.” 2017. Web. 07 Mar 2021.

Vancouver:

Cline P. Female Airline Transport Pilots: The Role of Mentoring. [Internet] [Doctoral dissertation]. University of North Dakota; 2017. [cited 2021 Mar 07]. Available from: https://commons.und.edu/theses/344.

Council of Science Editors:

Cline P. Female Airline Transport Pilots: The Role of Mentoring. [Doctoral Dissertation]. University of North Dakota; 2017. Available from: https://commons.und.edu/theses/344

9. Achterberg, Peter. Formation and breakdown of adenosine in the heart : investigations on myocardial purine metabolismen.

Degree: 1986, Erasmus University Medical Center

URL: http://hdl.handle.net/1765/39037

► textabstractAdenosine, a strong coronary vasodilator, is a breakdown product of the myocardial high-energy phosphate ATP. ATP serves as the direct energy source for contraction of… (more)

▼ textabstractAdenosine, a strong coronary vasodilator, is a breakdown product of the myocardial high-energy phosphate ATP. ATP serves as the direct energy source for contraction of the heart. Chapter 1 of this thesis gives a general introduction on contractility dependent ATP-breakdown, the ATP-generating metabolism of the heart and the mechanism which leads to adenosine formation. The applications of purine biochemistry to cardiovascular research are summarized. The in vitro characteristics of other enzymes of purine metabolism (AMP-deaminase and S-adenosylhomocysteine hydrolase) are reported. Additional perfusion studies with specific inhibitors suggest a significant contribution by these enzymes to myocardial purine formation during normoxia (Appendix Papers II and IV) . Several lines of evidence suggest that the purines released from isolated rat hearts are in part degradation products of IMP or GMP instead of AMP. This appears to be especially so during normoxia. If hypoxanthine or inosine are added to the myocardial perfusate, they are slowly incorporated into the ATP-pool of the heart. The incorporation rate is increased after a previous ischemic period and can be further stimulated by simultaneous infusion of ribose. Evidence is presented that increased adenosine formation is directly related to increased formation of AMP (e.g., from ATP). The old hypothesis that adenosine is constantly produced at a high rate and nearly simultaneously reincorporated into AMP appears to be unrealistic. The results suggest an even tighter coupling of adenosine and purine release to the myocardial energy state than had already been assumed. A minor disbalance between ATP-breakdown and ATP-production will lead to increased adenosine and purine release and will eventually lead to significant loss of ATP and myocardial function. Some of the experiments point to ways of breaking through this vicious circle by defining conditions for enhanced myocardial ATP-repletion and possible restoration of function during reperfusion

Subjects/Keywords: ATP; adenosine

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APA (6^th Edition):

Achterberg, P. (1986). Formation and breakdown of adenosine in the heart : investigations on myocardial purine metabolismen. (Doctoral Dissertation). Erasmus University Medical Center. Retrieved from http://hdl.handle.net/1765/39037

Chicago Manual of Style (16^th Edition):

Achterberg, Peter. “Formation and breakdown of adenosine in the heart : investigations on myocardial purine metabolismen.” 1986. Doctoral Dissertation, Erasmus University Medical Center. Accessed March 07, 2021. http://hdl.handle.net/1765/39037.

MLA Handbook (7^th Edition):

Achterberg, Peter. “Formation and breakdown of adenosine in the heart : investigations on myocardial purine metabolismen.” 1986. Web. 07 Mar 2021.

Vancouver:

Achterberg P. Formation and breakdown of adenosine in the heart : investigations on myocardial purine metabolismen. [Internet] [Doctoral dissertation]. Erasmus University Medical Center; 1986. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/1765/39037.

Council of Science Editors:

Achterberg P. Formation and breakdown of adenosine in the heart : investigations on myocardial purine metabolismen. [Doctoral Dissertation]. Erasmus University Medical Center; 1986. Available from: http://hdl.handle.net/1765/39037

University of Debrecen

10. Józsa, Réka Anna. A [(η6-p-cym)Ru(H2O)3]2+ kölcsönhatása ATP-vel .

Degree: DE – Természettudományi és Technológiai Kar – Kémiai Intézet, University of Debrecen

URL: http://hdl.handle.net/2437/226843

► Az ATP a DNS egyik alkotórésze, a négy nukleotid egyike, bizonyos esetekben az ATP DNS modellnek is tekinthető, így fiziológiás pH-n az esetlegesen kialakuló komplexek… (more)

Subjects/Keywords: Ruténium; ATP

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APA (6^th Edition):

Józsa, R. A. (n.d.). A [(η6-p-cym)Ru(H2O)3]2+ kölcsönhatása ATP-vel . (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/226843

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Józsa, Réka Anna. “A [(η6-p-cym)Ru(H2O)3]2+ kölcsönhatása ATP-vel .” Thesis, University of Debrecen. Accessed March 07, 2021. http://hdl.handle.net/2437/226843.

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Józsa, Réka Anna. “A [(η6-p-cym)Ru(H2O)3]2+ kölcsönhatása ATP-vel .” Web. 07 Mar 2021.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

Józsa RA. A [(η6-p-cym)Ru(H2O)3]2+ kölcsönhatása ATP-vel . [Internet] [Thesis]. University of Debrecen; [cited 2021 Mar 07]. Available from: http://hdl.handle.net/2437/226843.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

Council of Science Editors:

Józsa RA. A [(η6-p-cym)Ru(H2O)3]2+ kölcsönhatása ATP-vel . [Thesis]. University of Debrecen; Available from: http://hdl.handle.net/2437/226843

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

University of Illinois – Chicago

11. Tchernookova, Boriana Krassimirova. Characterization of Extracellular H+ Fluxes from Isolated Glia and Slices of the Vertebrate Retina.

Degree: 2017, University of Illinois – Chicago

URL: http://hdl.handle.net/10027/21812

► Active glia-neuron interactions are crucial for the proper development and function of the nervous system. Bidirectional communication between neurons and glial cells has been shown… (more)

▼ Active glia-neuron interactions are crucial for the proper development and function of the nervous system. Bidirectional communication between neurons and glial cells has been shown to have modulatory effects on neuronal signaling. The exact mechanisms which glial cells employ to influence the behavior of neurons have not been fully understood. In the vertebrate retina, extracellular pH can act as a modulator of neuronal signaling by altering the activity of calcium channels, particularly on photoreceptor terminals. Experiments have demonstrated that when the pH of the extracellular medium is lowered to more acidic values, the calcium flux into photoreceptors is lessened. The exact cell type, which mediates these changes in extracellular pH, is still debatable with mounting evidence pointing to the role of horizontal cells in this process. This work presents evidence that retina Müller glial cells of tiger salamander are capable of mediating changes in extracellular proton concentration. Müller glia respond to extracellular ATP with a robust extracellular acidification measured with ion-selective microelectrodes. The mechanism through which these cells change the extracellular acidity involves the activation of membrane ATP receptors and the mobilization of intracellular signaling molecules with resulting intracellular calcium increases. The ATP-induced sustained acidifications are observed from isolated Müller glia as well as in retinal slices. Interestingly, Müller cells of the retinae of many other vertebrates tested, including Macaques and human, also respond to extracellular ATP through acidifications of the extracellular space, suggesting that this is a conserved and potentially physiologically important response. Müller cells respond to extracellular glutamate as well, but the changes in extracellular acidity are opposite of these evoked by ATP: glutamate induces Müller cell-mediated extracellular transient alkalinization, which seems to be due to the activity of membrane glutamate transporters. In slices, the initial transient alkalinization induced by glutamate is followed by a pronounced, sustained acidification, whose mechanism is independent of the glutamate transporters’ activity. This data suggest Müller glia could potentially play a role in extracellular proton modulation of neuronal activity in the vertebrate retina Advisors/Committee Members: Malchow, Robert P (advisor), Park, Thomas (committee member), Gong, Liang-Wei (committee member), Matthew Kreitzer (committee member), Murphy, Alvin D (chair).

Subjects/Keywords: retina; glia; ATP; pH

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APA (6^th Edition):

Tchernookova, B. K. (2017). Characterization of Extracellular H+ Fluxes from Isolated Glia and Slices of the Vertebrate Retina. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/21812

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tchernookova, Boriana Krassimirova. “Characterization of Extracellular H+ Fluxes from Isolated Glia and Slices of the Vertebrate Retina.” 2017. Thesis, University of Illinois – Chicago. Accessed March 07, 2021. http://hdl.handle.net/10027/21812.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tchernookova, Boriana Krassimirova. “Characterization of Extracellular H+ Fluxes from Isolated Glia and Slices of the Vertebrate Retina.” 2017. Web. 07 Mar 2021.

Vancouver:

Tchernookova BK. Characterization of Extracellular H+ Fluxes from Isolated Glia and Slices of the Vertebrate Retina. [Internet] [Thesis]. University of Illinois – Chicago; 2017. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10027/21812.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tchernookova BK. Characterization of Extracellular H+ Fluxes from Isolated Glia and Slices of the Vertebrate Retina. [Thesis]. University of Illinois – Chicago; 2017. Available from: http://hdl.handle.net/10027/21812

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Minnesota

12. Robinson, Jerid. Activation and Inhibition Mechanisms of Membrane Guanylyl Cyclases.

Degree: PhD, Pharmacology, 2013, University of Minnesota

URL: http://hdl.handle.net/11299/173946

► Guanylyl cyclase (GC)-A and GC-B are homologous enzymes that catalyze the formation of cyclic guanosine monophosphate and pyrophosphate from GTP. GC-A is activated by atrial… (more)

Subjects/Keywords: ANP; ATP; Guanylyl Cyclase

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APA (6^th Edition):

Robinson, J. (2013). Activation and Inhibition Mechanisms of Membrane Guanylyl Cyclases. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/173946

Chicago Manual of Style (16^th Edition):

Robinson, Jerid. “Activation and Inhibition Mechanisms of Membrane Guanylyl Cyclases.” 2013. Doctoral Dissertation, University of Minnesota. Accessed March 07, 2021. http://hdl.handle.net/11299/173946.

MLA Handbook (7^th Edition):

Robinson, Jerid. “Activation and Inhibition Mechanisms of Membrane Guanylyl Cyclases.” 2013. Web. 07 Mar 2021.

Vancouver:

Robinson J. Activation and Inhibition Mechanisms of Membrane Guanylyl Cyclases. [Internet] [Doctoral dissertation]. University of Minnesota; 2013. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/11299/173946.

Council of Science Editors:

Robinson J. Activation and Inhibition Mechanisms of Membrane Guanylyl Cyclases. [Doctoral Dissertation]. University of Minnesota; 2013. Available from: http://hdl.handle.net/11299/173946

13. Julkunen, Henna. Käyttöohje voilinjan CIP-pesuprosessista.

Degree: 2011, Seinäjoen ammattikorkeakoulu

URL: http://www.theseus.fi/handle/10024/32275

►

Opinnäytetyön tavoitteena oli tehdä yksityiskohtainen käyttöohje uuden rasvatehtaan voilinjan CIP- pesuprosessista ja sen toiminnasta sekä ohjauksesta. Käyttöohje tehtiin Valio Oy:n Seinäjoen rasvatehtaalle. Käyttöohjeen tarkoitus on… (more)

▼

Opinnäytetyön tavoitteena oli tehdä yksityiskohtainen käyttöohje uuden rasvatehtaan voilinjan CIP- pesuprosessista ja sen toiminnasta sekä ohjauksesta. Käyttöohje tehtiin Valio Oy:n Seinäjoen rasvatehtaalle. Käyttöohjeen tarkoitus on antaa selkeät toimintaohjeet turvalliseen pesutoimintoon sekä parhaan pesutuloksen saavuttamiseen. Käyttöohje opastaa rutiininomaisesti valvomon työntekijöitä ja yhtenäistää heidän toimintatapojaan. Käyttöohje antaa myös tukea itsenäiseen työskentelyyn valvomossa. Ennen uuden tehtaan valmistumista pesuprosessin toiminnasta ja ohjauksesta vastasivat kokeneet valvomotyöntekijät. Uuden organisaatiomuutoksen myötä myös voinvalmistajat työskentelevät ajoittain valvomossa, joten käyttöohjeita eri toiminnoista pyydettiin tukemaan heidän valvomotyöskentelyään. Käyttöohjeen lisäksi työssä perehdyttiin perusteellisemmin elintarvikkeita koskeviin hygieniavaatimuksiin sekä elintarviketeollisuuden kaupalliseen, moraaliseen ja lainmukaiseen velvoitteeseen. Työssä käsiteltiin myös käsitteet: steriili, bakteriologinen sekä kemiallinen puhtaus. Työssä käytiin läpi myös ATP- menetelmällä otettuja puhdistustuloksia voilinjalta, jonka pesuun käyttöohje on tehty.

The object for the thesis was to create a detailed operation manual for operators on CIP washing process in the new butter plant. The manual includes all functions and controls to operate CIP washing process that is used to clean manufacturing lines in the butter plant. The manual was made to Valio PLC, Seinäjoki. The purpose of the manual is to provide clear instructions for a safe washing process and the best possible washing result. The manual is created to advise operators in the control room and to standardise their way of working. The manual is also made to provide support to operators in the control room. Before the new plant was built, experienced control room operators were responsible for the control of the CIP washing process. Due to changes in the new organization, now butter makers are responsible for CIP washing process and a well detailed operation manual is needed. In addition to the operation manual, the thesis studies also food hygiene and commercial, moral and legal obligation of food industry. Also the concepts sterile, bacteriological and chemical cleanliness are handled. The cleanliness tests from butter manufacturing lines taken with ATP- method are part of the thesis as well.

Advisors/Committee Members: Seinäjoen ammattikorkeakoulu.

Subjects/Keywords: elintarvikehygienia; pesu; CIP; ATP

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APA (6^th Edition):

Julkunen, H. (2011). Käyttöohje voilinjan CIP-pesuprosessista. (Thesis). Seinäjoen ammattikorkeakoulu. Retrieved from http://www.theseus.fi/handle/10024/32275

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Julkunen, Henna. “Käyttöohje voilinjan CIP-pesuprosessista.” 2011. Thesis, Seinäjoen ammattikorkeakoulu. Accessed March 07, 2021. http://www.theseus.fi/handle/10024/32275.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Julkunen, Henna. “Käyttöohje voilinjan CIP-pesuprosessista.” 2011. Web. 07 Mar 2021.

Vancouver:

Julkunen H. Käyttöohje voilinjan CIP-pesuprosessista. [Internet] [Thesis]. Seinäjoen ammattikorkeakoulu; 2011. [cited 2021 Mar 07]. Available from: http://www.theseus.fi/handle/10024/32275.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Julkunen H. Käyttöohje voilinjan CIP-pesuprosessista. [Thesis]. Seinäjoen ammattikorkeakoulu; 2011. Available from: http://www.theseus.fi/handle/10024/32275

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Louisiana State University

14. Hendrix, Amanda Yoesting. Base-caged Adenosine Triphosphate as a Model System for Photoactivatable Small Interfering RNA.

Degree: MSBAE, Engineering, 2012, Louisiana State University

URL: etd-06062012-164102 ; https://digitalcommons.lsu.edu/gradschool_theses/527

► Photocaged adenosine triphosphate (ATP) is one of the earliest examples of exerting spatial-temporal control over the activity of a substrate. The activity of ATP is… (more)

Subjects/Keywords: competitive inhibition; photoactivation; ATP; caged

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APA (6^th Edition):

Hendrix, A. Y. (2012). Base-caged Adenosine Triphosphate as a Model System for Photoactivatable Small Interfering RNA. (Masters Thesis). Louisiana State University. Retrieved from etd-06062012-164102 ; https://digitalcommons.lsu.edu/gradschool_theses/527

Chicago Manual of Style (16^th Edition):

Hendrix, Amanda Yoesting. “Base-caged Adenosine Triphosphate as a Model System for Photoactivatable Small Interfering RNA.” 2012. Masters Thesis, Louisiana State University. Accessed March 07, 2021. etd-06062012-164102 ; https://digitalcommons.lsu.edu/gradschool_theses/527.

MLA Handbook (7^th Edition):

Hendrix, Amanda Yoesting. “Base-caged Adenosine Triphosphate as a Model System for Photoactivatable Small Interfering RNA.” 2012. Web. 07 Mar 2021.

Vancouver:

Hendrix AY. Base-caged Adenosine Triphosphate as a Model System for Photoactivatable Small Interfering RNA. [Internet] [Masters thesis]. Louisiana State University; 2012. [cited 2021 Mar 07]. Available from: etd-06062012-164102 ; https://digitalcommons.lsu.edu/gradschool_theses/527.

Council of Science Editors:

Hendrix AY. Base-caged Adenosine Triphosphate as a Model System for Photoactivatable Small Interfering RNA. [Masters Thesis]. Louisiana State University; 2012. Available from: etd-06062012-164102 ; https://digitalcommons.lsu.edu/gradschool_theses/527

University of Canterbury

15. Mittelstädt, Gerd Horst. Allosteric regulation of the adenosine triphosphate phosphoribosyltransferase from campylobacter jejuni.

Degree: PhD, Biochemistry, 2015, University of Canterbury

URL: http://dx.doi.org/10.26021/9208

► The enzyme adenosine triphosphate phosphoribosyltransferase (ATP-PRT) catalyses the first reaction of the histidine biosynthetic pathway. ATP-PRT also represents a metabolic control point, directing the flux… (more)

▼ The enzyme adenosine triphosphate phosphoribosyltransferase (ATP-PRT) catalyses the first reaction of the histidine biosynthetic pathway. ATP-PRT also represents a metabolic control point, directing the flux of metabolites through this energetically expensive pathway. Two distinctly different forms of ATP-PRT exist, the long form and the short form, which differ in the presence of a C-terminal regulatory domain. In the short form, where this domain is absent, it is functionally replaced by a regulatory protein, called HisZ. ATP-PRT activity is modulated by two layers of regulation: active site inhibition by adenosine monophosphate, which reflects cellular energy levels, and pathway end product feedback inhibition by histidine. In the long form ATP-PRT histidine binds to the allosteric site at the regulatory domain, but the exact nature of the inhibitory mechanism is still debated. This thesis characterises a new member of the ATP-PRT long form from Campylobacter jejuni (CjeATP-PRT) and investigates the molecular mechanisms involved in the feed back inhibition by histidine. Chapter 2 describes the characterisation of the CjeATP-PRT including a detailed description of its crystal structure. The C. jejuni enzyme is similar to the previously described enzymes of the ATP-PRT long form, but exists only as hexameric species under experimental conditions, which contradicts previous assumptions that the hexamer is exclusively inactive. Chapter 3 investigates the catalytic apparatus of CjeATP-PRT by separating the catalytic and regulatory domains of the enzyme for individual study. The isolated catalytic portion of the enzyme, the CjeATP-PRT Core mutant, forms a dimeric species with very limited catalytic capabilities but high substrate and product affnities. The CjeATP-PRT Core characteristics suggest that it exists in a permanently inhibited conformation, highlighting the requirement of the regulatory domain not only for feedback regulation but also for enzyme function. Additionally this supports the evolutionary need for the recruitment of a regulatory apparatus. In chapter 4 a potential intramolecular communication pathway from the allosteric to the active site is probed by the generation of several single site mutations. One of these, CjeATP-PRT R216A, is completely insensitive to histidine inhibition, although this ligand is still able to bind at the allosteric site, which is consistent with the involvement of R216 in the allosteric signal communication. The catalytic abilities of CjeATP-PRT R216A are largely impaired, leading to the assumption that this mutation causes a permanent inhibitory response. In summary this thesis supports the existence of a simple physical regulatorymechanism for the feedback inhibition of the ATP-PRT long form, the change between two different hexamer conformations depending on the presence of the allosteric effector.

Subjects/Keywords: ATP-PRT; Structure; Regulation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mittelstädt, G. H. (2015). Allosteric regulation of the adenosine triphosphate phosphoribosyltransferase from campylobacter jejuni. (Doctoral Dissertation). University of Canterbury. Retrieved from http://dx.doi.org/10.26021/9208

Chicago Manual of Style (16^th Edition):

Mittelstädt, Gerd Horst. “Allosteric regulation of the adenosine triphosphate phosphoribosyltransferase from campylobacter jejuni.” 2015. Doctoral Dissertation, University of Canterbury. Accessed March 07, 2021. http://dx.doi.org/10.26021/9208.

MLA Handbook (7^th Edition):

Mittelstädt, Gerd Horst. “Allosteric regulation of the adenosine triphosphate phosphoribosyltransferase from campylobacter jejuni.” 2015. Web. 07 Mar 2021.

Vancouver:

Mittelstädt GH. Allosteric regulation of the adenosine triphosphate phosphoribosyltransferase from campylobacter jejuni. [Internet] [Doctoral dissertation]. University of Canterbury; 2015. [cited 2021 Mar 07]. Available from: http://dx.doi.org/10.26021/9208.

Council of Science Editors:

Mittelstädt GH. Allosteric regulation of the adenosine triphosphate phosphoribosyltransferase from campylobacter jejuni. [Doctoral Dissertation]. University of Canterbury; 2015. Available from: http://dx.doi.org/10.26021/9208

University of Texas – Austin

16. -7326-3122. Investigation of the role of extracellular nucleotide gradients in plant gravity responses.

Degree: PhD, Plant biology, 2016, University of Texas – Austin

URL: http://hdl.handle.net/2152/46510

► Extracellular ATP (eATP) was first identified as a neurotransmitter in animal systems decades ago, but has only recently been classified as a signaling molecule in… (more)

▼ Extracellular ATP (eATP) was first identified as a neurotransmitter in animal systems decades ago, but has only recently been classified as a signaling molecule in plants. Previous studies have shown that exogenously applied ATP can disrupt gravitropism in roots, depolarize root hairs, and alter auxin distribution. These results support a clear role for this molecule as a regulatory signal in plants. To further define eATP as a signal in plants, Ceratopteris spores, a model system, were used to study gravity-directed cell polarization. This polarization begins with the uptake of Ca2+ through channels at the bottom of the spore, a process required for the cell’s gravity response. Previous data showing that mechanosensitive channels can release ATP and that eATP can induce the opening of Ca2+ channels led to the hypothesis that eATP could play a role in the gravity-directed polarization. Data described in this dissertation show that an eATP gradient, with significantly higher [ATP] outside the bottom of the cell, is present during and promotes gravity-directed polarization. To explore the link between eATP and Ca2+ in gravity-directed polarization of spores, microparticle bombardment was used to transform Ceratopteris cells with a FRET-based Ca2+ sensor, Yellow Cameleon 3.60. The success of this effort has generated a uniquely valuable tool that can be used to analyze intracellular Ca2+ dynamics and rapidly screen transformants in Ceratopteris, a primitive plant system. In addition to studying the role of eATP signaling in the gravity response of single cells, an assessment of its role in the gravity response of a multicellular system, primary roots of Arabidopsis, was carried out. By using ecto-luciferase-expressing seedlings, a gradient of eATP, with the highest concentration being along the bottom of the root, was visualized within 30 min of gravistimulation. When this gradient was disrupted by excess ATP or an eATP receptor antagonist, the gravity response was attenuated. These results characterize the role of eATP gradients in the gravity responses of single spore cells of ferns and multicellular primary roots of a flowering plant. They suggest that the preferential accumulation of eATP along the bottom of gravity-responding cells is an evolutionarily conserved mechanism for promoting gravity-directed development. Advisors/Committee Members: Roux, Stanley J. (advisor), Browning, Karen S (committee member), Huq, Enamul (committee member), Levin, Donald A (committee member), Mehdy, Mona (committee member).

Subjects/Keywords: Extracellular ATP; Gravity; Ceratopteris; Arabidopsis

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APA (6^th Edition):

-7326-3122. (2016). Investigation of the role of extracellular nucleotide gradients in plant gravity responses. (Doctoral Dissertation). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/46510

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Chicago Manual of Style (16^th Edition):

-7326-3122. “Investigation of the role of extracellular nucleotide gradients in plant gravity responses.” 2016. Doctoral Dissertation, University of Texas – Austin. Accessed March 07, 2021. http://hdl.handle.net/2152/46510.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

MLA Handbook (7^th Edition):

-7326-3122. “Investigation of the role of extracellular nucleotide gradients in plant gravity responses.” 2016. Web. 07 Mar 2021.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

-7326-3122. Investigation of the role of extracellular nucleotide gradients in plant gravity responses. [Internet] [Doctoral dissertation]. University of Texas – Austin; 2016. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/2152/46510.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Council of Science Editors:

-7326-3122. Investigation of the role of extracellular nucleotide gradients in plant gravity responses. [Doctoral Dissertation]. University of Texas – Austin; 2016. Available from: http://hdl.handle.net/2152/46510

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

University of Sydney

17. Selvamani, Sakthi. Effect of Hepatitis B and C Viruses on Mitochondrial Function .

Degree: 2020, University of Sydney

URL: http://hdl.handle.net/2123/24376

► HCV and HBV infections are leading causes of chronic hepatitis, cirrhosis, and hepatocellular carcinoma and can induce metabolic dysfunction, which may offer a selective advantage… (more)

▼ HCV and HBV infections are leading causes of chronic hepatitis, cirrhosis, and hepatocellular carcinoma and can induce metabolic dysfunction, which may offer a selective advantage for liver cancer proliferation and survival. The liver is enriched with numerous mitochondria, providing a continuous supply of ATP for a range of cellular activities. The hypothesis of this thesis is that HBV and HCV induce mitochondrial dysfunction and metabolic disorders. A range of cell culture models and in vitro techniques were used to test this hypothesis, including the Seahorse analyser to measure mitochondrial function in real time. Mitochondrial function and membrane potential during HCV and HBV infection were decreased, which were independent of mitochondrial biogenesis. HCV infection promotes steatosis due to a combination of viral and host metabolic factors, whereas steatosis during HBV is more variable. Therefore, potential changes in lipid metabolism were investigated in our HCV and HBV models. Impaired lipid oxidation was observed during HCV infection, but not during HBV expression. Perturbation of pyruvate metabolism was proposed as a possible mechanism for mitochondrial dysfunction during HBV expression. No significant change in pyruvate but an increase in lactate concentrations was observed, due to elevated lactate dehydrogenase A, which converts pyruvate to lactate. Proteomics analysis revealed other key proteins involved in pyruvate metabolism to be differentially regulated, including increased levels of pyruvate dehydrogenase kinase. In summary, HCV infection causes mitochondrial dysfunction and reduced lipid oxidation, resulting in intracellular accumulation of lipids. In contrast, HBV expression does not affect lipids but alters pyruvate metabolism, causing lactate accumulation and promoting the “Warburg effect”. Thus, although HBV does not cause steatosis, the lactate accumulation and altered cell metabolism may promote the development and progression of liver cancer.

Subjects/Keywords: HBV; HCV; Mitochondria; steatosis; ATP

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Selvamani, S. (2020). Effect of Hepatitis B and C Viruses on Mitochondrial Function . (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/24376

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Selvamani, Sakthi. “Effect of Hepatitis B and C Viruses on Mitochondrial Function .” 2020. Thesis, University of Sydney. Accessed March 07, 2021. http://hdl.handle.net/2123/24376.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Selvamani, Sakthi. “Effect of Hepatitis B and C Viruses on Mitochondrial Function .” 2020. Web. 07 Mar 2021.

Vancouver:

Selvamani S. Effect of Hepatitis B and C Viruses on Mitochondrial Function . [Internet] [Thesis]. University of Sydney; 2020. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/2123/24376.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Selvamani S. Effect of Hepatitis B and C Viruses on Mitochondrial Function . [Thesis]. University of Sydney; 2020. Available from: http://hdl.handle.net/2123/24376

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Tampere University

18. Kontro, Heidi. Regulating the ATP Synthase Activity - Characterization of the F-ATP synthase subunit DAPIT and its over-expression in HEK293T cells .

Degree: 2017, Tampere University

URL: https://trepo.tuni.fi/handle/10024/101020

► Tyypin 1 diabetes on monimutkainen metabolinen häiriötila, joka aiheuttaa muutoksia solun fysiologiaan, aineenvaihduntaan ja geenien ilmentymiseen. Insuliinin puutoksen tiedetään aiheuttavan hiiren kudoksissa geenisäätelyn häiriintymistä mm.… (more)

▼ Tyypin 1 diabetes on monimutkainen metabolinen häiriötila, joka aiheuttaa muutoksia solun fysiologiaan, aineenvaihduntaan ja geenien ilmentymiseen. Insuliinin puutoksen tiedetään aiheuttavan hiiren kudoksissa geenisäätelyn häiriintymistä mm. laskemalla mitokondriaalisen elektroninsiirtoketjun proteiinien mRNA-tasoja. Sen sijaan energiaa tuottavan ATP-syntaasin rakenneyksiköiden ilmentyminen diabeteksessa on proteiinitasolla vielä tuntematonta, kuten ilmentymisen vaikutukset itse entsyymikompleksin aktiivisuuteenkin. Tässä väitöskirjatyössä on löydetty ja karakterisoitu ATP-syntaasin Fo kompleksin rakenneproteiini Diabetes Associated Protein in Insulin-sensitive Tissues, DAPIT. Lisäksi sen yli-ilmentymisen vaikutusta solun fysiologiaan on luonnehdittu molekyylibiologisin keinoin diabeettisen ja muun tautiyhteyden selventämiseksi. Väitöskirja koostuu kolmesta erillisestä osatyöstä. Osatyössä I DD-PCR-, sekvensointi-, Northern blot-menetelmien sekä tietokantahaun avulla löydettiin, nimettiin ja karakterisoitiin uusi proteiini, DAPIT, tyypin 1 diabeettisen rotan insuliiniherkistä kudoksista. Osatyössä II karakterisoitiin erikoisvalmisteinen, DAPITin tunnistava vasta-aine, jonka avulla proteiinin ilmentyminen havainnollistettiin immunohistokemiallisesti useissa rotan ja ihmisen kudoksissa. Lisäksi vahvistettiin DAPITin mitokondriaalinen esiintyminen solutasolla, sekä havaittiin lysosomaalinen ilmentyminen. DAPITin ilmentymistä tutkittiin myös diabeettisen rotan ja hiiren insuliiniherkissä kudoksissa, ja havainnollistettiin sen lihassäie-spesifinen esiintyminen hiiressä. Väitöskirjan osatyössä III geenimuuntelun avulla rakennettiin DAPITia yli-ilmentävä solulinja, jonka mitokondrioita, energia-aineenvaihduntaa, morfologiaa ja käyttäytymisominaisuuksia selvitettiin. Osatyö I tuotti uuden DAPIT-proteiinin, joka osoittautui konservoituneeksi eläinkunnassa. Terveellä rotalla proteiinin lähetti-RNA ilmeni runsaana mm. insuliiniherkissä kudoskissa. Diabeettisella rotalla lähetti-RNAn ilmentyminen oli alentunut sydämessä ja lihaksessa kontrollikudokseen verrattuna. Osatyössä II DAPITin aminohapposekvenssin karboksyylipäähän suunniteltu vasta-aine tunnisti kohteensa sekä solu- että kudostasolla. Vasta-aineen avulla nähtiin DAPITin erityinen ilmentyminen mitokondrioissa ja lysosomeissa. Aminopäähän suunniteltu vasta-aine ei ollut yhtä luotettava jokaisessa sovelluksessa. Ihmisen ja rotan kudosten immunohistokemiallisessa tutkimuksessa DAPITia esiintyi runsaasti aerobista aineenvaihduntaa hyödyntävissä insuliiniherkissä kudoksissa, sekä aktiivisesti ravinteita ja ioneja kuljettavissa epiteelirakenteissa. Diabeettisen rotan insuliiniherkkien kudosten tutkimus osoitti DAPITin proteiinitason olevan koholla, toisin kuin lähetti-RNA tasolla, sydämessä, lihaksessa ja rasvassa mutta alentunut maksassa normaaliin kudokseen verrattuna. Terveen ja diabeettisen hiiren lihaksessa eroja sen sijaan ei havaittu lihasyhdistelmän runsaan säietyyppivaihtelun vuoksi. Osatyössä III DAPITia aktivoidusti ilmentävässä solulinjassa havaittiin…

Subjects/Keywords: DAPIT ; ATP-syntaasi ; mitokondria ; metabolia ; ATP synthase ; mitochondria ; metabolism

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kontro, H. (2017). Regulating the ATP Synthase Activity - Characterization of the F-ATP synthase subunit DAPIT and its over-expression in HEK293T cells . (Doctoral Dissertation). Tampere University. Retrieved from https://trepo.tuni.fi/handle/10024/101020

Chicago Manual of Style (16^th Edition):

Kontro, Heidi. “Regulating the ATP Synthase Activity - Characterization of the F-ATP synthase subunit DAPIT and its over-expression in HEK293T cells .” 2017. Doctoral Dissertation, Tampere University. Accessed March 07, 2021. https://trepo.tuni.fi/handle/10024/101020.

MLA Handbook (7^th Edition):

Kontro, Heidi. “Regulating the ATP Synthase Activity - Characterization of the F-ATP synthase subunit DAPIT and its over-expression in HEK293T cells .” 2017. Web. 07 Mar 2021.

Vancouver:

Kontro H. Regulating the ATP Synthase Activity - Characterization of the F-ATP synthase subunit DAPIT and its over-expression in HEK293T cells . [Internet] [Doctoral dissertation]. Tampere University; 2017. [cited 2021 Mar 07]. Available from: https://trepo.tuni.fi/handle/10024/101020.

Council of Science Editors:

Kontro H. Regulating the ATP Synthase Activity - Characterization of the F-ATP synthase subunit DAPIT and its over-expression in HEK293T cells . [Doctoral Dissertation]. Tampere University; 2017. Available from: https://trepo.tuni.fi/handle/10024/101020

19. Rita Gabriela Pedrosa Ribeiro Mendes. Identificação de alvos antigênicos na ATP difosfohidrolase solúvel de Schistosoma mansoni e possível aplicação funcional de peptídeos sintéticos.

Degree: 2010, Universidade Federal de Juiz de Fora

URL: http://www.bdtd.ufjf.br/tde_busca/arquivo.php?codArquivo=921

► The antigenicity of both soluble S. mansoni ATP diphosphohydrolase and membraneassociated isoforms has been recently described. Cross-immunoreactivity between potato apyrase and IgG antibody from S.… (more)

▼ The antigenicity of both soluble S. mansoni ATP diphosphohydrolase and membraneassociated isoforms has been recently described. Cross-immunoreactivity between potato apyrase and IgG antibody from S. mansoni-infected mice or schistosomiasis patients strongly suggested that the epitopes shared between the vegetable and parasite proteins are antigenic. By bioinformatics analysis, a strategic comparative study of the conserved domains shared between the soluble ATP diphosphohydrolase isoform and potato apyrase predicted a homologue antigenic-domain available for antibody and HLA-DR binding. Synthetic peptides belonging to the domain of the parasite (Sm) and vegetable (pot) proteins were then obtained. By ELISA, the serum samples from the schistosomiasis patients (n= 146) showed high seropositivity of IgG1 (58%) and IgG4 (58%) antibody sub-classes for potato apyrase. Seropositive patients (n= 35) were then selected for analyses of antibody reactivity against the synthetic peptides. Significant seropositivity of IgG (potB1LJ, 9%; potB1MP, 54%; potB2LJ, 54%; SmB1LJ, 23%; SmB1MP, 86%; SmB2LJ, 34%), and IgG1 (potB1LJ, 57%; potB1MP, 67%; potB2LJ, 46%; SmB1LJ, 57%; SmB1MP, 10%; SmB2LJ, 31%) or IgG4 (potB1LJ, 71%; potB1MP, 40%; potB2LJ, 71%; SmB1LJ, 54%; SmB1MP, 63%; SmB2LJ, 49%) was observed for the homologue peptides in accordance with the existence of shared antigenic epitopes between the parasite and vegetable proteins, and demonstrating that in schistosomiasis the domain r155-194 from soluble ATP diphosphohydrolase isoform is rich in B-cell epitopes. The reactivity of the IgG1 and IgG4 antibody from the serum samples of patients (n= 26) cured after chemotherapy with praziquantel was analyzed against potB1LJ e potB2LJ, before and six months after treatment. Individual analysis revealed variable IgG1 and IgG4 reactivity against each peptide, suggesting that different antigenic epitopes could be involved in susceptibility or resistance to S. mansoni reinfection. By ELISA, polyclonal antibody to potato apyrase of BALB/c or CBA/J mice reacts with potB1LJ, potB1MP and/or potB2LJ. Similar reactivity was also observed between these immune sera and SmB1LJ, SmB1MP and/or SmB2LJ, confirming again the cross-immunoreactivity between parasite and vegetable proteins. In addition, inoculation of each peptide in healthy Swiss mice had significant immunostimulatory activity, increasing the amount of total IgG and/or IgG1 and IgG2a antibody, as observed by ELISA and/or Dot blots, suggesting that each peptide has two distinct epitopes capable of inducing a Th1 or Th2-type immune response. By Western blots, serum immune anti-SmB2LJ recognized two polypeptides of approximately 63 and 55 kDa in SEA or SWAP, indicating the presence of the soluble isoform of ATP diphosphohydrolase in these antigenic preparations, as well as the sensitivity and specificity of this immune serum. The potato apyrase and potB1LJ were evaluated in delayed-type hypersensitivity reaction (DTH), using ovalbumin (OVA) as standard antigen, by footpad thickness measurement,… Advisors/Committee Members: Eveline Gomes Vasconcelos, José Otávio do Amaral Correa, Florence Mara Rosa.

Subjects/Keywords: Schistosoma mansoni; Esquistossomose; Atp Difosfohidrolase; Schistosoma mansoni; schistosomiasis; ATP diphosphohydrolase; IMUNOLOGIA

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mendes, R. G. P. R. (2010). Identificação de alvos antigênicos na ATP difosfohidrolase solúvel de Schistosoma mansoni e possível aplicação funcional de peptídeos sintéticos. (Thesis). Universidade Federal de Juiz de Fora. Retrieved from http://www.bdtd.ufjf.br/tde_busca/arquivo.php?codArquivo=921

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mendes, Rita Gabriela Pedrosa Ribeiro. “Identificação de alvos antigênicos na ATP difosfohidrolase solúvel de Schistosoma mansoni e possível aplicação funcional de peptídeos sintéticos.” 2010. Thesis, Universidade Federal de Juiz de Fora. Accessed March 07, 2021. http://www.bdtd.ufjf.br/tde_busca/arquivo.php?codArquivo=921.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mendes, Rita Gabriela Pedrosa Ribeiro. “Identificação de alvos antigênicos na ATP difosfohidrolase solúvel de Schistosoma mansoni e possível aplicação funcional de peptídeos sintéticos.” 2010. Web. 07 Mar 2021.

Vancouver:

Mendes RGPR. Identificação de alvos antigênicos na ATP difosfohidrolase solúvel de Schistosoma mansoni e possível aplicação funcional de peptídeos sintéticos. [Internet] [Thesis]. Universidade Federal de Juiz de Fora; 2010. [cited 2021 Mar 07]. Available from: http://www.bdtd.ufjf.br/tde_busca/arquivo.php?codArquivo=921.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mendes RGPR. Identificação de alvos antigênicos na ATP difosfohidrolase solúvel de Schistosoma mansoni e possível aplicação funcional de peptídeos sintéticos. [Thesis]. Universidade Federal de Juiz de Fora; 2010. Available from: http://www.bdtd.ufjf.br/tde_busca/arquivo.php?codArquivo=921

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

20. Kabala, Anna Magdalena. Biogenesis of mitochondrial ATP synthase and its dysfunction leading to diseases : Biogenese de l’ATP synthase mitochondriale et des dysfonctions générant des maladies.

Degree: Docteur es, Biochimie, 2014, Bordeaux; ACADEMIE POLONAISE DES SCIENCES

URL: http://www.theses.fr/2014BORD0366

►

La F1FO-ATP synthase mitochondriale produit la majorité de l’énergie cellulaire chezles eucaryotes aérobes sous forme d’ATP par le processus des oxydations phosphorylantes.Chez la plupart des… (more)

▼

La F1FO-ATP synthase mitochondriale produit la majorité de l’énergie cellulaire chezles eucaryotes aérobes sous forme d’ATP par le processus des oxydations phosphorylantes.Chez la plupart des espèces, cette enzyme possède une origine génétique double, nucléaire etmitochondriale. Dans la première partie de ce travail, je décris la construction de modèles delevure de mutations du gène mitochondrial ATP6 de l’ATP synthase découvertes chez despatients atteints de maladies neurologiques (9185T>C and 9191T>C) ou dans des tumeurs(8716A>G, 8914C>A, 8932C>T, 8953A>G and 9131T>C). Le gène ATP6 code une sousunitéessentielle (a/6) du domaine FO de l’ATP synthase. J’ai trouvé que la mutation 9185T>Cn’affecte pas l’assemblage de l’ATP synthase, mais conduit à une diminution de la vitesse desynthèse d’ATP d’environ 30%. La mutation 9191T>C empêche presque entièrementl’incorporation de la sous-unité a/6 dans l’ATP synthase. Les cinq mutations identifiées dansles tumeurs ont un effet modeste à nul, indiquant que ces mutations ne favorisent pas latumorigenèse en affectant le processus énergétique mitochondrial, comme évoquéprécédemment. J’ai ensuite étudié la régulation de la synthèse des sous-unités a/6 et 9 dans lesmitochondries de levures. La sous-unité 9 est présente sous la forme d’un anneau de 10 copiesqui interagit avec la sous-unité 6. Durant la catalyse, la rotation de cet anneau provoque deschangements conformationnels favorisant la synthèse d’ATP dans le secteur F1 de l’ATPsynthase. Je montre que la synthèse de ces protéines est couplée à leur assemblage, demanière à ce qu’elles soient produites dans une stoechiométrie adéquate et pour éviterl’accumulation d’intermédiaires d’ATP synthase potentiellement délétères

Mitochondrial F1FO-ATP synthase produces most of the cellular energy in aerobiceukaryotes under the form of ATP in the process of oxidative phosphorylation. This enzymehas in most species a double genetic origin, nuclear and mitochondrial. In the first part of thiswork, I describe the construction of yeast models of ATP synthase mutations in themitochondrial ATP6 gene, that have been found in patients presenting with neurologicaldisorders (9185T>C and 9191T>C) and in tumors (8716A>G, 8914C>A, 8932C>T,8953A>G and 9131T>C). The ATP6 gene encodes an essential subunit (called a/6) of theATP synthase proton-translocating domain (FO). The 9185T>C mutation had no effect on theassembly of ATP synthase, but reduces the rate of ATP synthesis by 30%. The 9191T>Cmutation almost completely prevented incorporation of the subunit a/6 into the ATP synthase.The five mutations found in tumors had modest, if at all, effect, indicating that thesemutations probably do not favor tumorigenesis, as was hypothesized. In the second part of mythesis, I studied the regulation of synthesis of subunits a/6 and 9 in yeast mitochondria. Thesubunit 9 is present in 10 copies forming a ring that interacts with subunit 6. Protonmovements through the FO induce the rotation of the subunit 9-ring, which results inconformational changes that promote…

Advisors/Committee Members: Di Rago, Jean-Paul (thesis director), Kucharczyk, Roza (thesis director).

Subjects/Keywords: ATP synthase; Syndrome NARP; Cancer; Biogenese de l’ATP synthase; ATP synthase; NARP syndrome; Cancer; Biogenesis of ATP synthase

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kabala, A. M. (2014). Biogenesis of mitochondrial ATP synthase and its dysfunction leading to diseases : Biogenese de l’ATP synthase mitochondriale et des dysfonctions générant des maladies. (Doctoral Dissertation). Bordeaux; ACADEMIE POLONAISE DES SCIENCES. Retrieved from http://www.theses.fr/2014BORD0366

Chicago Manual of Style (16^th Edition):

Kabala, Anna Magdalena. “Biogenesis of mitochondrial ATP synthase and its dysfunction leading to diseases : Biogenese de l’ATP synthase mitochondriale et des dysfonctions générant des maladies.” 2014. Doctoral Dissertation, Bordeaux; ACADEMIE POLONAISE DES SCIENCES. Accessed March 07, 2021. http://www.theses.fr/2014BORD0366.

MLA Handbook (7^th Edition):

Kabala, Anna Magdalena. “Biogenesis of mitochondrial ATP synthase and its dysfunction leading to diseases : Biogenese de l’ATP synthase mitochondriale et des dysfonctions générant des maladies.” 2014. Web. 07 Mar 2021.

Vancouver:

Kabala AM. Biogenesis of mitochondrial ATP synthase and its dysfunction leading to diseases : Biogenese de l’ATP synthase mitochondriale et des dysfonctions générant des maladies. [Internet] [Doctoral dissertation]. Bordeaux; ACADEMIE POLONAISE DES SCIENCES; 2014. [cited 2021 Mar 07]. Available from: http://www.theses.fr/2014BORD0366.

Council of Science Editors:

Kabala AM. Biogenesis of mitochondrial ATP synthase and its dysfunction leading to diseases : Biogenese de l’ATP synthase mitochondriale et des dysfonctions générant des maladies. [Doctoral Dissertation]. Bordeaux; ACADEMIE POLONAISE DES SCIENCES; 2014. Available from: http://www.theses.fr/2014BORD0366

Ruhr Universität Bochum

21. Sojka, Johann Sebastian. Interaktion vasokonstriktiver P2X- und P2Y-Rezeptoren in der Mäuseniere.

Degree: 2010, Ruhr Universität Bochum

URL: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-27646

► Das sympathische Nervensystem ist an der Regulation des renovaskulären Widerstands beteiligt. Noradrenalin und ATP vermitteln dabei als Neurotransmitter vasokonstriktive Effekte. Postsynaptische Wirkungen von Noradrenalin und… (more)

Subjects/Keywords: ATP; Essentielle Hypertonie; Niere; Noradrenalin; Nucleotide

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APA (6^th Edition):

Sojka, J. S. (2010). Interaktion vasokonstriktiver P2X- und P2Y-Rezeptoren in der Mäuseniere. (Thesis). Ruhr Universität Bochum. Retrieved from http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-27646

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sojka, Johann Sebastian. “Interaktion vasokonstriktiver P2X- und P2Y-Rezeptoren in der Mäuseniere.” 2010. Thesis, Ruhr Universität Bochum. Accessed March 07, 2021. http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-27646.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sojka, Johann Sebastian. “Interaktion vasokonstriktiver P2X- und P2Y-Rezeptoren in der Mäuseniere.” 2010. Web. 07 Mar 2021.

Vancouver:

Sojka JS. Interaktion vasokonstriktiver P2X- und P2Y-Rezeptoren in der Mäuseniere. [Internet] [Thesis]. Ruhr Universität Bochum; 2010. [cited 2021 Mar 07]. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-27646.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sojka JS. Interaktion vasokonstriktiver P2X- und P2Y-Rezeptoren in der Mäuseniere. [Thesis]. Ruhr Universität Bochum; 2010. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-27646

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Ruhr Universität Bochum

22. Wagenhäuser, Peter Johannes. Einfluß purinerger P2Y2-Rezeptoren auf die Progredienz der chronischen Niereninsuffizienz am Mausmodell.

Degree: 2011, Ruhr Universität Bochum

URL: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-32936

► Methode: Bei Wildtyp- (WT-) und P2Y2-Knockout- (KO-) Mäusen wurde durch 5/6-Nephrektomie (SNx) eine Niereninsuffizienz induziert. Bei Kontrollgruppen wurden die Nieren in situ belassen (Sham). Postoperativ… (more)

Subjects/Keywords: Nephrektomie; Knockout (Molekulargenetik); Nierenkrankheit; Purinozeptor; ATP

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wagenhäuser, P. J. (2011). Einfluß purinerger P2Y2-Rezeptoren auf die Progredienz der chronischen Niereninsuffizienz am Mausmodell. (Thesis). Ruhr Universität Bochum. Retrieved from http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-32936

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wagenhäuser, Peter Johannes. “Einfluß purinerger P2Y2-Rezeptoren auf die Progredienz der chronischen Niereninsuffizienz am Mausmodell.” 2011. Thesis, Ruhr Universität Bochum. Accessed March 07, 2021. http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-32936.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wagenhäuser, Peter Johannes. “Einfluß purinerger P2Y2-Rezeptoren auf die Progredienz der chronischen Niereninsuffizienz am Mausmodell.” 2011. Web. 07 Mar 2021.

Vancouver:

Wagenhäuser PJ. Einfluß purinerger P2Y2-Rezeptoren auf die Progredienz der chronischen Niereninsuffizienz am Mausmodell. [Internet] [Thesis]. Ruhr Universität Bochum; 2011. [cited 2021 Mar 07]. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-32936.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wagenhäuser PJ. Einfluß purinerger P2Y2-Rezeptoren auf die Progredienz der chronischen Niereninsuffizienz am Mausmodell. [Thesis]. Ruhr Universität Bochum; 2011. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-32936

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

23. Martins, Rodrigo Machado. A transposição didática do papel termodinâmico do ATP gera conceitos alternativos?.

Degree: Mestrado, Bioquímica, 2012, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/46/46131/tde-26042013-092432/ ;

►

Conceitos prévios, ou alternativos, são ideias introjetadas no universo cognitivo dos estudantes que diferem daqueles credenciados pela ciência estabelecida. São bastante estudadas as consequências da… (more)

▼

Conceitos prévios, ou alternativos, são ideias introjetadas no universo cognitivo dos estudantes que diferem daqueles credenciados pela ciência estabelecida. São bastante estudadas as consequências da presença deste tipo de conceitos para o aprendizado. Entre outras dificuldades provocadas pelos conceitos alternativos está a impossibilidade de utilizá-los para embasar novos conhecimentos. Nestas condições, incapazes de decodificar apropriadamente as novas informações apresentadas pelas disciplinas, os alunos são inconscientemente encaminhados para a memorização. A origem dos conceitos prévios é variada. Uma das causas já detectadas é a precariedade da transposição didática, feita pelos autores dos livros textos ou por docentes. Uma das moléculas fundamentais para os estudos bioquímicos é a adenosina trifosfato (ATP), que desempenha múltiplas funções. Uma das principais é participar de processos que requerem energia. A compreensão do papel desta molécula é fundamental para o entendimento dos processos dos quais ela participa. Um dos objetivos do presente trabalho foi investigar equívocos sobre o papel termodinâmico do ATP nos processos celulares. Os testes foram realizados com alunos do ensino médio (EM), graduação (G) e pós-graduação (PG). A existência de concepções alternativas foi verificada, assim como sua estabilidade nos diferentes níveis de escolaridade: um resultado mostra que 68% EM, 92% G e 91% PG afirmaram que a energia da hidrólise de ATP é responsável por conduzir os processos celulares. Os resultados gerais mostram que os estudantes carregam equívocos em conceitos termodinâmicos básicos, tais como transferência de energia e espontaneidade de reações químicas. Duas possíveis fontes de conceitos alternativos da termodinâmica do ATP são o professor e o livro didático. Nesse trabalho foi verificado que os livros de ensino médio e graduação podem contribuir para a instalação de conceitos alternativos referentes ao ATP. Nos livros analisados, principalmente os de ensino médio, foram encontrados passagens, analogias e esquemas que podem contribuir para isso. E por fim, uma interferência didática foi feita com o intuito de corrigir o entendimento dos alunos no que diz respeito ao papel do ATP nas reações químicas. Alunos monitorados por meio de pré e pós-testes apresentaram resultados animadores em relação à atenuação de conceitos relacionados à termodinâmica do ATP. Dos alunos que participaram da intervenção, mais de 80% responderam e justificaram corretamente os testes feitos pós-intervenção

Misconceptions, or alternative concepts, are introjected ideas in the students` cognitive universe that differ from those established by science. The consequences of alternative concepts for the learning process are widely studied. Among other difficulties caused by alternative concepts is the impossibility to use them to support new knowledge. Under these conditions, unable to properly decode the new information submitted by the disciplines, students are unconsciously driven to memorization. The origin of the…

Advisors/Committee Members: Torres, Bayardo Baptista.

Subjects/Keywords: Alternative concepts about ATP; Biochemistry (Study and teaching); Bioquímica (Estudo e ensino); Conceitos alternativos sobre o ATP; Didactic transposition (ATP); Termodinâmica (Conceitos alternativos); Thermodynamic (Alternative concepts); Transposição (Didática ATP)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Martins, R. M. (2012). A transposição didática do papel termodinâmico do ATP gera conceitos alternativos?. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/46/46131/tde-26042013-092432/ ;

Chicago Manual of Style (16^th Edition):

Martins, Rodrigo Machado. “A transposição didática do papel termodinâmico do ATP gera conceitos alternativos?.” 2012. Masters Thesis, University of São Paulo. Accessed March 07, 2021. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-26042013-092432/ ;.

MLA Handbook (7^th Edition):

Martins, Rodrigo Machado. “A transposição didática do papel termodinâmico do ATP gera conceitos alternativos?.” 2012. Web. 07 Mar 2021.

Vancouver:

Martins RM. A transposição didática do papel termodinâmico do ATP gera conceitos alternativos?. [Internet] [Masters thesis]. University of São Paulo; 2012. [cited 2021 Mar 07]. Available from: http://www.teses.usp.br/teses/disponiveis/46/46131/tde-26042013-092432/ ;.

Council of Science Editors:

Martins RM. A transposição didática do papel termodinâmico do ATP gera conceitos alternativos?. [Masters Thesis]. University of São Paulo; 2012. Available from: http://www.teses.usp.br/teses/disponiveis/46/46131/tde-26042013-092432/ ;

McMaster University

24. Ortiz Castro, Mary. Characterizing the interactions of ATP and DNA with the MutL Mismatch Repair protein.

Degree: MSc, 2016, McMaster University

URL: http://hdl.handle.net/11375/20279

►

The fidelity of DNA replication prevents mutations that may lead to cancer predisposition or neurodegenerative diseases. One mechanism that enhances DNA replication fidelity is DNA… (more)

Subjects/Keywords: MutL; mismatch repair; ATP; DNA; endonuclease activity

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ortiz Castro, M. (2016). Characterizing the interactions of ATP and DNA with the MutL Mismatch Repair protein. (Masters Thesis). McMaster University. Retrieved from http://hdl.handle.net/11375/20279

Chicago Manual of Style (16^th Edition):

Ortiz Castro, Mary. “Characterizing the interactions of ATP and DNA with the MutL Mismatch Repair protein.” 2016. Masters Thesis, McMaster University. Accessed March 07, 2021. http://hdl.handle.net/11375/20279.

MLA Handbook (7^th Edition):

Ortiz Castro, Mary. “Characterizing the interactions of ATP and DNA with the MutL Mismatch Repair protein.” 2016. Web. 07 Mar 2021.

Vancouver:

Ortiz Castro M. Characterizing the interactions of ATP and DNA with the MutL Mismatch Repair protein. [Internet] [Masters thesis]. McMaster University; 2016. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/11375/20279.

Council of Science Editors:

Ortiz Castro M. Characterizing the interactions of ATP and DNA with the MutL Mismatch Repair protein. [Masters Thesis]. McMaster University; 2016. Available from: http://hdl.handle.net/11375/20279

University of Toronto

25. Gao, Andrew Feng. The Role of ATP and P2X Purinoceptor 7 in the Development of Cerebral Tau.

Degree: 2018, University of Toronto

URL: http://hdl.handle.net/1807/91436

►

ATP release and signalling via the P2X7 receptor have been implicated in numerous pathological processes including neurodegeneration and may play a role in the development… (more)

Subjects/Keywords: ATP; Neurodegeneration; P2X7R; Tau; Tauopathy; 0317

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gao, A. F. (2018). The Role of ATP and P2X Purinoceptor 7 in the Development of Cerebral Tau. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/91436

Chicago Manual of Style (16^th Edition):

Gao, Andrew Feng. “The Role of ATP and P2X Purinoceptor 7 in the Development of Cerebral Tau.” 2018. Masters Thesis, University of Toronto. Accessed March 07, 2021. http://hdl.handle.net/1807/91436.

MLA Handbook (7^th Edition):

Gao, Andrew Feng. “The Role of ATP and P2X Purinoceptor 7 in the Development of Cerebral Tau.” 2018. Web. 07 Mar 2021.

Vancouver:

Gao AF. The Role of ATP and P2X Purinoceptor 7 in the Development of Cerebral Tau. [Internet] [Masters thesis]. University of Toronto; 2018. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/1807/91436.

Council of Science Editors:

Gao AF. The Role of ATP and P2X Purinoceptor 7 in the Development of Cerebral Tau. [Masters Thesis]. University of Toronto; 2018. Available from: http://hdl.handle.net/1807/91436

26. 이, 진삼. ATP depletion at G2 phase blocks mitotic entry through maintaining inhibitory phosphorylation of CDK1.

Degree: 2018, Ajou University

URL: http://repository.ajou.ac.kr/handle/201003/16540 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000026806

►

Tumor cells have been reported to have two main energy checkpoints depending on the amount of available ATP. These checkpoints regulate the progression of tumor… (more)

▼

Tumor cells have been reported to have two main energy checkpoints depending on the amount of available ATP. These checkpoints regulate the progression of tumor cells during the G1 phase and their progression from the G2/M phase. Thus, the cell cycle depends on the time needed to produce enough ATP to overcome the energy checkpoints. Although molecular mechanism of G1 energy checkpoint has been reported, G2 energy checkpoint has not been fully explained. Here, I have shown that ATP depletion at G2 phase blocks both mitotic entry and cyclin B nucleus translocation in multiple cell lines. In addition, G2-M transition was regulated by the level of intracellular ATP. ATP depletion maintains p-Cdk1 (Y15), which is widely known to be phosphorylated by Wee1 kinase. Moreover, ATP depletion at G2 phase induced p-Chk1 (S345). Thus, I treated Wee1 inhibitor, PD166285, during ATP depletion at G2 phase. PD166285 dramatically overcome G2 arrest by ATP depletion. In addition, I also treated DNA damage response signaling inhibitor to target Chk1. However, DDR signaling inhibitor did not overcome G2 arrest by ATP depletion. Experiment using chemical inhibition and siRNA for AMPK also revealed that ATP depletion-induced G2 arrest did not result from AMPK activation. Taken together, ATP depletion at G2 phase blocks mitotic entry through maintaining inhibitory phosphorylation of Cdk1. Moreover, I observed that some the G2 arrested cells treated with PD166285 eventually died, indicating potential therapeutic implication and Wee1 inhibition upon ATPdepleted cells.

진핵세포는 다양한 스트레스에 반응하여 세포주기를 조절하는checkpoint를 가지고 있다. 진핵세포의 세포주기에서 이용 가능한 ATP의 양에 따라 2 가지 주요 energy checkpoint를 갖는 것으로 보고되었다. 이 checkpoint는 G1 기 동안 종양 세포주기의 진행과 G2 / M transition의 진행을 조절한다. G1 energy checkpoint에서는 AMPK에 의해 cyclin E가 억제되는 molecular mechanism이 보고되었지만 G2 energy checkpoint는 아직 완전히설명되지 않았다. 본 저자는 G2 기에서 ATP 결핍이 세포주기에 어떤영향을 미치는지 알아보고자 하였다. ATP를 결핍 시키는데 사용한 약물은 glycolysis를 억제하는 2-DG와 mitochondrial Cytochrome C oxidase를 억제하는 NaN3를 사용하였다. Drugs를 처리하였을 때 ATP가 asynchronous 수준으로 떨어지는 것을 확인하였다. 그 다음 mitotic entry와 cyclin B nuclear translocation을 확인한 결과 mitotic entry와 cyclin B nuclear translocation이 차단된 것을 관찰할 수 있었다. 이러한 현상이 drugs을 wash out 해 주었을 때 정상수준으로 회복되는지 알아보고자 하였다. 그 결과 drugs를 wash out 했을 때 ATP와 mitotic index가 정상 수준으로 회복하였다. 그 다음 세포주기가 멈추는 현상이 정말로 ATP결핍에 의한 현상인지 알아보고자 또 다른 energy stress를 가해주었다. 2-DG와 mitochondrial complex I 을 억제하는 phenformin을 동시에 처리한 결과 세포주기가 정지되는 것을 확인하였다. 그 다음 glucose free media로 glucose starvation 시켰을 때에도 이러한 현상이 일어나는지 확인하였다. 그 결과 drugs 처리 때보다는 약한정도 였으나 어느정도 세포분열기로의 이행이 억제되는 것을 볼 수 있었다. 그 다음 세포내 ATP를 측정한 결과 2-DG와 phenformin을 처리하였을 때는 asynchronous 수준으로 떨어지는 것을 확인하였다. 하지만 glucose starvation 조건에서는 ATP가 조금 감소하는 정도였다. 따라서 세포내 ATP의 양에 따라 세포주기가 조절된다는 것을 알 수 있었다. 이러한 현상이 일어나는 기전으로 먼저 AMPK에 의한 것인지 알아보고자 하였다. siRNA와 inhibitor를 사용하여 AMPK를 억제하였지만 세포주기 진행억제가 극복되지 않았다. 따라서 G2 energy checkpoint에서 AMPK는 큰 영향을 미치지 않는다는 것을 확인하였다. 그 다음 DNA 손상에 의한 신호전달 경로를 확인한 결과 Chk1이 인산화 되는 것을 확인하였다. 다양한 inhibitor를 통해 Chk1의 인산화를 억제하였지만 세포주기 진행억제를 극복하지 못했다. 따라서 Chk1도 별다른 영향을 미치지 않는다는…

Advisors/Committee Members: 대학원 의생명과학과, 201524774, 이, 진삼.

Subjects/Keywords: ATP depletion; Cdk1; Wee1; Energy-checkpoint

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

이, �. (2018). ATP depletion at G2 phase blocks mitotic entry through maintaining inhibitory phosphorylation of CDK1. (Thesis). Ajou University. Retrieved from http://repository.ajou.ac.kr/handle/201003/16540 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000026806

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

이, 진삼. “ATP depletion at G2 phase blocks mitotic entry through maintaining inhibitory phosphorylation of CDK1.” 2018. Thesis, Ajou University. Accessed March 07, 2021. http://repository.ajou.ac.kr/handle/201003/16540 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000026806.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

이, 진삼. “ATP depletion at G2 phase blocks mitotic entry through maintaining inhibitory phosphorylation of CDK1.” 2018. Web. 07 Mar 2021.

Vancouver:

이 �. ATP depletion at G2 phase blocks mitotic entry through maintaining inhibitory phosphorylation of CDK1. [Internet] [Thesis]. Ajou University; 2018. [cited 2021 Mar 07]. Available from: http://repository.ajou.ac.kr/handle/201003/16540 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000026806.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

이 �. ATP depletion at G2 phase blocks mitotic entry through maintaining inhibitory phosphorylation of CDK1. [Thesis]. Ajou University; 2018. Available from: http://repository.ajou.ac.kr/handle/201003/16540 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000026806

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Ottawa

27. Cloutier, Adam. Initiation of Solution NMR Studies on the Bacterial Cell Division Regulator MinD .

Degree: 2019, University of Ottawa

URL: http://hdl.handle.net/10393/39664

► Bacterial cell division relies on the cell division septum to form at the mid-cell position. In gram negative bacteria, this is mediated by three proteins,… (more)

▼ Bacterial cell division relies on the cell division septum to form at the mid-cell position. In gram negative bacteria, this is mediated by three proteins, MinC, MinD and MinE. Together these proteins interact with each other and the membrane in a dynamic, oscillating process which prevents cell division septum formation at the cell poles. The early phase of this process involves MinD binding to the membrane, which is triggered upon binding of ATP. Subsequent interactions with MinE result in stimulation of the ATPase activity of MinD. After hydrolysis, MinD is released from the membrane and diffuses to a new binding site. Many in silico models have been constructed of the Min system in an attempt to describe its self-organizing behaviour. A limitation of these models is that, in order to prevent rapid re-binding of MinD to the membrane after hydrolysis of ATP, the exchange of bound ADP for ATP is assumed to be a slow process, on the order of 1/s . In order to provide experimental evidence of the rate of nucleotide binding, we performed a series of triple-resonance NMR experiments to complete a partial assignment of backbone atom resonances, which required the application of deuterium labelling and amino acid-specific selective unlabelling. After the introduction of ATP, it was discovered that no dimerization had been induced, in contrast with existing literature. It was proposed that MinD from N. gonorrhoeae only forms a dimer in the presence of a membrane, while literature with MinD from E. coli shows it does not have this requirement. Interestingly while dimerization had not been induced, there was a persistent population of dimeric species even in the absence of nucleotide. This was discovered to be the result of disulfide formation, likely an artifact of established purification protocols. Binding of both ADP and ATP to MinD were studied by titration using NMR, with the relative affinity of both nucleotides to MinD being indistinguishable. By analyzing peak coalescence in the half-bound condition, a maximum rate was determined for nucleotide binding, with the lifetime being on the order of 170ms. Results from this experiment support models requiring a slow nucleotide binding step, and help enhance understanding of how Min proteins sustain oscillations required for normal cell division.

Subjects/Keywords: NMR; MinD; ATP; Dimer; Exchange; N. gonorrhoeae

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cloutier, A. (2019). Initiation of Solution NMR Studies on the Bacterial Cell Division Regulator MinD . (Thesis). University of Ottawa. Retrieved from http://hdl.handle.net/10393/39664

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cloutier, Adam. “Initiation of Solution NMR Studies on the Bacterial Cell Division Regulator MinD .” 2019. Thesis, University of Ottawa. Accessed March 07, 2021. http://hdl.handle.net/10393/39664.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cloutier, Adam. “Initiation of Solution NMR Studies on the Bacterial Cell Division Regulator MinD .” 2019. Web. 07 Mar 2021.

Vancouver:

Cloutier A. Initiation of Solution NMR Studies on the Bacterial Cell Division Regulator MinD . [Internet] [Thesis]. University of Ottawa; 2019. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10393/39664.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cloutier A. Initiation of Solution NMR Studies on the Bacterial Cell Division Regulator MinD . [Thesis]. University of Ottawa; 2019. Available from: http://hdl.handle.net/10393/39664

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Miami

28. Qiu, Feng. Regulation of Pannexin 1 Channels by ATP.

Degree: PhD, Physiology and Biophysics (Medicine), 2010, University of Miami

URL: https://scholarlyrepository.miami.edu/oa_dissertations/394

► Pannexins represent a recently discovered second family of gap junction proteins in vertebrates. However, instead of forming intercellular gap junction channels like connexins, pannexins… (more)

▼ Pannexins represent a recently discovered second family of gap junction proteins in vertebrates. However, instead of forming intercellular gap junction channels like connexins, pannexins operate as unpaired pannexons, allowing the flux of molecules from the cytoplasm to the extracellular space and vice versa. Pannexins appear to play a vital role in the local control loop of blood perfusion and oxygen delivery. The properties of Panx1 channels indicate that this protein is the most probable candidate for an ATP release channel and is involved in the propagation of intercellular calcium waves. It is also proposed to mediate the large pore formation of the P2X7 receptor death complex. Prolonged activation of this receptor can lead to cell death. There must be some mechanisms to stop this ATP-induced ATP release and opening of the lethal pore. Here we describe a negative feedback loop controlling pannexin 1 channel activity. ATP, permeant to pannexin 1 channels, was found to inhibit its permeation pathway when applied extracellularly. ATP analogues, including BzATP, suramine, and BBG were even more effective inhibitors of pannexin 1 currents than ATP. These compounds also attenuated the uptake of dyes by erythrocytes, which express pannexin 1. The rank order of the compounds in attenuation of pannexin 1 currents was similar to their binding affinities to the P2X7 receptor, except that receptor agonists and antagonists both were inhibitory to the channel. The ATP inhibitory effect is largely decreased when R75 on the first extracellular loop of Pannexin1 is mutated to alanine, strongly indicating that the ATP regulates this channel through binding. To further investigate the structural property of the ATP binding, we did alanine scanning mutagenesis of the extracellular loops and found that mutations on W74, S237, S240, I247 and L266 on the extracellular loops severely impair the BzATP inhibitory effect indicating that they might be direct binding partners for the ligands. Mutations on R75, S82, S93, L94, D241, S249, P259 and I267 have largely decreased BzATP sensitivity. Mutations on other residues didn't change the BzATP sensitivity compared to the wild type except for some nonfunctional mutants. All these data demonstrate that some amino acid residues on the extracellular loop of Pannexin 1 mediate ATP sensitivity. However, how these residues form the ATP-binding pocket remains to be elucidated. Advisors/Committee Members: Wolfgang Nonner, Grace Zhai, Ana Diez-Sampedro, Gerhard Dahl, Daniela Boassa.

Subjects/Keywords: Pannexin 1; ATP; Negative Feedback; P2X7 Receptor

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❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Qiu, F. (2010). Regulation of Pannexin 1 Channels by ATP. (Doctoral Dissertation). University of Miami. Retrieved from https://scholarlyrepository.miami.edu/oa_dissertations/394

Chicago Manual of Style (16^th Edition):

Qiu, Feng. “Regulation of Pannexin 1 Channels by ATP.” 2010. Doctoral Dissertation, University of Miami. Accessed March 07, 2021. https://scholarlyrepository.miami.edu/oa_dissertations/394.

MLA Handbook (7^th Edition):

Qiu, Feng. “Regulation of Pannexin 1 Channels by ATP.” 2010. Web. 07 Mar 2021.

Vancouver:

Qiu F. Regulation of Pannexin 1 Channels by ATP. [Internet] [Doctoral dissertation]. University of Miami; 2010. [cited 2021 Mar 07]. Available from: https://scholarlyrepository.miami.edu/oa_dissertations/394.

Council of Science Editors:

Qiu F. Regulation of Pannexin 1 Channels by ATP. [Doctoral Dissertation]. University of Miami; 2010. Available from: https://scholarlyrepository.miami.edu/oa_dissertations/394

University of Miami

29. Hsu, Byron Che-Fu. Analysis of Extracellular ATP Distribution in the Intervertebral Disc Using a Finite Element Model.

Degree: MS, Biomedical Engineering (Engineering), 2014, University of Miami

URL: https://scholarlyrepository.miami.edu/oa_theses/486

► Extracellular adenosine triphosphate (ATP) released from cells can mediate a diverse number of biological responses, such as cell secretion, inflammation, and immune reactions through… (more)

Subjects/Keywords: IVD; ATP; compression; Michaelis-Menten; numerical simulation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hsu, B. C. (2014). Analysis of Extracellular ATP Distribution in the Intervertebral Disc Using a Finite Element Model. (Thesis). University of Miami. Retrieved from https://scholarlyrepository.miami.edu/oa_theses/486

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hsu, Byron Che-Fu. “Analysis of Extracellular ATP Distribution in the Intervertebral Disc Using a Finite Element Model.” 2014. Thesis, University of Miami. Accessed March 07, 2021. https://scholarlyrepository.miami.edu/oa_theses/486.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hsu, Byron Che-Fu. “Analysis of Extracellular ATP Distribution in the Intervertebral Disc Using a Finite Element Model.” 2014. Web. 07 Mar 2021.

Vancouver:

Hsu BC. Analysis of Extracellular ATP Distribution in the Intervertebral Disc Using a Finite Element Model. [Internet] [Thesis]. University of Miami; 2014. [cited 2021 Mar 07]. Available from: https://scholarlyrepository.miami.edu/oa_theses/486.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hsu BC. Analysis of Extracellular ATP Distribution in the Intervertebral Disc Using a Finite Element Model. [Thesis]. University of Miami; 2014. Available from: https://scholarlyrepository.miami.edu/oa_theses/486

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Miami

30. Fernando, Hanan Nirosha. Mechanical Loading Affects the Energy Metabolism of Intervertebral Disc Cells.

Degree: MS, Biomedical Engineering (Engineering), 2010, University of Miami

URL: https://scholarlyrepository.miami.edu/oa_theses/286

► Back pain is the second most common neurological ailment in the United States and the leading cause of pain and disability. More than 80%… (more)

▼ Back pain is the second most common neurological ailment in the United States and the leading cause of pain and disability. More than 80% of the total US population experiences back-pain during their life time and the annual back pain related healthcare costs exceed 100 billion dollars. While the exact cause of low back pain (LBP) is still unknown, degeneration of the intervertebral disc (IVD) has been suggested as a primary contributor. IVD is the largest avascular tissue in the human body and it is composed of three integrated tissues (annulus fibrosus - AF, nucleus pulposus - NP and cartilaginous end plate - CEP). IVD functions as a shock-absorber during motion and provides flexibility to motion of the spine. Maintaining IVD tissue integrity is an energy demanding process. Studies have shown that mechanical loading affects cellular biosynthesis of IVD tissue and may also promote IVD degeneration. However the path to this effect is still unknown. We propose a link between mechanical loading and cell energy production which contributes to altered cellular biosynthesis. Thus, we investigated the effects of mechanical loading on IVD cell energy metabolism under various mechanical loading regimes. Porcine AF and NP cells were isolated and seeded in 2% agarose at a 5,000,000 cells/mL cell density. A custom made bioreactor was used to conduct compression experiments. The experiment groups were: 15% static compression; 30% static compression; 0.1, 1 and 2 Hz dynamic compression at 15% strain magnitude. Experiment duration was 4 hr. ATP concentration in cell-agarose construct and culture media were measured using Luciferin-luciferase method to evaluate ATP production and ATP release from cells respectively. Lactate concentration in media was measured using lactate dehydrogenase enzymatic assay. Nitrite (stable metabolite of nitric oxide - NO) concentration in media was measured by Greiss Assay. DNA content per sample was measured using fluorometric assay. DNA content per sample was used as an internal control; all compressed samples were then normalized to unstrained control group. ATP production of AF cells was up regulated by static and dynamic mechanical loading. Data suggests that AF cell response to mechanical loading is primarily loading amplitude dependent. NP cells exhibited an increased ATP production at 1 Hz dynamic loading but remained comparable to control samples at other tested conditions. AF cells produced an increase in NO production at 1-, 2 Hz dynamic loading. NO production of NP cells was up regulated by mechanical loading at all tested conditions. ATP release was up regulated at higher frequencies in AF cells. In addition to higher frequencies (1 Hz and 2 Hz) NP cell ATP release was also up regulated by 30% static compression. Thus, this study clearly illustrates that mechanical loading affects IVD cell energy production. Advisors/Committee Members: Chun-Yuh (Charles) Huang, Salahadin Abdi, Weiyong Gu.

Subjects/Keywords: ATP; Energy Metabolism; Mechanical Loading; Intervetebral Disc

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❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fernando, H. N. (2010). Mechanical Loading Affects the Energy Metabolism of Intervertebral Disc Cells. (Thesis). University of Miami. Retrieved from https://scholarlyrepository.miami.edu/oa_theses/286

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fernando, Hanan Nirosha. “Mechanical Loading Affects the Energy Metabolism of Intervertebral Disc Cells.” 2010. Thesis, University of Miami. Accessed March 07, 2021. https://scholarlyrepository.miami.edu/oa_theses/286.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fernando, Hanan Nirosha. “Mechanical Loading Affects the Energy Metabolism of Intervertebral Disc Cells.” 2010. Web. 07 Mar 2021.

Vancouver:

Fernando HN. Mechanical Loading Affects the Energy Metabolism of Intervertebral Disc Cells. [Internet] [Thesis]. University of Miami; 2010. [cited 2021 Mar 07]. Available from: https://scholarlyrepository.miami.edu/oa_theses/286.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fernando HN. Mechanical Loading Affects the Energy Metabolism of Intervertebral Disc Cells. [Thesis]. University of Miami; 2010. Available from: https://scholarlyrepository.miami.edu/oa_theses/286

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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Open Access Theses and Dissertations