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1. Abad Sánchez, Sergi. Biotechnological production of polyunsaturated fatty acids omega-3 by heterotrophic microalgae.

Degree: 2017, Universitat Ramon Llull

This dissertation describes the development of a biotechnological process to obtain polyunsaturated fatty acids (PUFA) omega-3 by heterotrophic microalgae. The selected heterotrophic microorganism was Aurantiochytrium limacinum SR21 due to its capacity to produce PUFA, grow with different carbon sources and tolerate high salinity. During the thesis investigation, diverse analytical methods have been developed in order to monitor A. limacinum growth. Moreover, different experimental design strategies/tools, such us Taguchi orthogonal matrices, Artificial neural network, Response surface methodology, etc. have been used to develop a growth medium specifically optimized for A. limacinum. Instead of using traditional carbon sources, the process has been developed to grow A. limacinum with crude glycerol, an industrial by‐product. The same experimental design tools served to find the best oxygen supply conditions to stimulate either biomass or DHA production. Batch, fed‐batch, continuous and multi‐stage continuous bioreactors have been studied to find the most prolific strategy. Batch and fed‐batch reactor generated the highest DHA yields (g DHA /g biomass). However, continuous cultivations produced higher DHA productivity values DHA (g DHA/l·h), especially the multi‐stage strategy. In a multi‐stage, continuous bioreactor, the first tank/s were dedicated to biomass production whereas the following tanks were set to stimulate DHA production. DHA products currently in market are formulated as DHA methyl esters or re‐esterified triglycerides, but not as unmodified triglycerides. In this thesis, an approach for preparative and process scale chromatography purification of unmodified triglycerides containing DHA has been developed. Advisors/Committee Members: Universitat Ramon Llull. IQS SE - Bioenginyeria, [email protected] (authoremail), true (authoremailshow), Turon Casalprim, Xavier (director), Planas Sauter, Antoni (codirector), true (authorsendemail).

Subjects/Keywords: Omega-3; Microalgae; Glycerol; Polyunsaturated fatty acids; Bioreactor; Batch; Continuous reactor; Cultivation; Analytical chemistry; Growth Medium; Ciències naturals, químiques, físiques i matemàtiques; 543; 577; 579; 633; 663/664

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APA (6th Edition):

Abad Sánchez, S. (2017). Biotechnological production of polyunsaturated fatty acids omega-3 by heterotrophic microalgae. (Thesis). Universitat Ramon Llull. Retrieved from http://hdl.handle.net/10803/400556

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Abad Sánchez, Sergi. “Biotechnological production of polyunsaturated fatty acids omega-3 by heterotrophic microalgae.” 2017. Thesis, Universitat Ramon Llull. Accessed February 21, 2020. http://hdl.handle.net/10803/400556.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Abad Sánchez, Sergi. “Biotechnological production of polyunsaturated fatty acids omega-3 by heterotrophic microalgae.” 2017. Web. 21 Feb 2020.

Vancouver:

Abad Sánchez S. Biotechnological production of polyunsaturated fatty acids omega-3 by heterotrophic microalgae. [Internet] [Thesis]. Universitat Ramon Llull; 2017. [cited 2020 Feb 21]. Available from: http://hdl.handle.net/10803/400556.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Abad Sánchez S. Biotechnological production of polyunsaturated fatty acids omega-3 by heterotrophic microalgae. [Thesis]. Universitat Ramon Llull; 2017. Available from: http://hdl.handle.net/10803/400556

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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