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University of Bradford

1. Khaghani, Seyed Ali. Cell and tissue engineering of articular cartilage via regulation and alignment of primary chondrocyte using manipulated transforming growth factors and ECM proteins : effect of transforming growth factor-beta (TGF-β1, 2 and 3) on the biological regulation and wound repair of chondrocyte monolayers with and without presence of ECM proteins.

Degree: PhD, 2010, University of Bradford

Articular cartilage is an avascular and flexible connective tissue found in joints. It produces a cushioning effect at the joints and provides low friction to protect the ends of the bones from wear and tear/damage. It has poor repair capacity and any injury can result pain and loss of mobility. One of the common forms of articular cartilage disease which has a huge impact on patient's life is arthritis. Research on cartilage cell/tissue engineering will help patients to improve their physical activity by replacing or treating the diseased/damaged cartilage tissue. Cartilage cell, called chondrocyte is embedded in the matrix (Lacunae) and has round shape in vivo. The in vitro monolayer culture of primary chondrocyte causes morphological change characterized as dedifferentiation. Transforming growth factor-beta (TGF-β), a cytokine superfamily, regulates cell function, including differentiation and proliferation. The effect of TGF-β1, 2, 3, and their manipulated forms in biological regulation of primary chondrocyte was investigated in this work. A novel method was developed to isolate and purify the primary chondrocytes from knee joint of neonate Sprague-Dawley rat, and the effect of some supplementations such as hyaluronic acid and antibiotics were also investigated to provide the most appropriate condition for in vitro culture of chondrocyte cells. Addition of 0.1mg/ml hyaluronic acid in chondrocyte culture media resulted an increase in primary chondrocyte proliferation and helped the cells to maintain chondrocytic morphology. TGF-β1, 2 and 3 caused chondrocytes to obtain fibroblastic phenotype, alongside an increase in apoptosis. The healing process of the wound closure assay of chondrocyte monolayers were slowed down by all three isoforms of TGF-β. All three types of TGF-β negatively affected the strength of chondrocyte adhesion. TGF-β1, 2 and 3 up regulated the expression of collagen type-II, but decreased synthesis of collagen type-I, Chondroitin sulfate glycoprotein, and laminin. They did not show any significant change in production of S-100 protein and fibronectin. TGF-β2, and 3 did not change expression of integrin-β1 (CD29), but TGF-β1 decreased the secretion of this adhesion protein. Manipulated TGF-β showed huge impact on formation of fibroblast like morphology of chondrocytes with chondrocytic phenotype. These isoforms also decreased the expression of laminin, chondroitin sulfate glycoprotein, and collagen type-I, but they increased production of collagen type-II and did not induce synthesis of fibronectin and S-100 protein. In addition, the strength of cell adhesion on solid surface was reduced by manipulated TGF-β. Only manipulated form of TGF-β1 and 2 could increase the proliferation rate. Manipulation of TGF-β did not up regulate the expression of integrin-β1 in planar culture system. The implications of this R&D work are that the manipulation of TGF-β by combination of TGF-β1, 2, and 3 can be utilized in production of superficial zone of cartilage and perichondrium. The collagen, fibronectin and…

Subjects/Keywords: 611; Primary chondrocyte cell; Cell / Tissue engineering; Monolayer cell culture; Pellet culture; Cell marker; Wound closure assay; Integrin-?1 (CD29); TGF-?1; TGF-?2; TGF-?3; Articular cartilage; Growth factors

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Khaghani, S. A. (2010). Cell and tissue engineering of articular cartilage via regulation and alignment of primary chondrocyte using manipulated transforming growth factors and ECM proteins : effect of transforming growth factor-beta (TGF-β1, 2 and 3) on the biological regulation and wound repair of chondrocyte monolayers with and without presence of ECM proteins. (Doctoral Dissertation). University of Bradford. Retrieved from http://hdl.handle.net/10454/5371

Chicago Manual of Style (16th Edition):

Khaghani, Seyed Ali. “Cell and tissue engineering of articular cartilage via regulation and alignment of primary chondrocyte using manipulated transforming growth factors and ECM proteins : effect of transforming growth factor-beta (TGF-β1, 2 and 3) on the biological regulation and wound repair of chondrocyte monolayers with and without presence of ECM proteins.” 2010. Doctoral Dissertation, University of Bradford. Accessed May 26, 2019. http://hdl.handle.net/10454/5371.

MLA Handbook (7th Edition):

Khaghani, Seyed Ali. “Cell and tissue engineering of articular cartilage via regulation and alignment of primary chondrocyte using manipulated transforming growth factors and ECM proteins : effect of transforming growth factor-beta (TGF-β1, 2 and 3) on the biological regulation and wound repair of chondrocyte monolayers with and without presence of ECM proteins.” 2010. Web. 26 May 2019.

Vancouver:

Khaghani SA. Cell and tissue engineering of articular cartilage via regulation and alignment of primary chondrocyte using manipulated transforming growth factors and ECM proteins : effect of transforming growth factor-beta (TGF-β1, 2 and 3) on the biological regulation and wound repair of chondrocyte monolayers with and without presence of ECM proteins. [Internet] [Doctoral dissertation]. University of Bradford; 2010. [cited 2019 May 26]. Available from: http://hdl.handle.net/10454/5371.

Council of Science Editors:

Khaghani SA. Cell and tissue engineering of articular cartilage via regulation and alignment of primary chondrocyte using manipulated transforming growth factors and ECM proteins : effect of transforming growth factor-beta (TGF-β1, 2 and 3) on the biological regulation and wound repair of chondrocyte monolayers with and without presence of ECM proteins. [Doctoral Dissertation]. University of Bradford; 2010. Available from: http://hdl.handle.net/10454/5371

2. Gallel Vicente, María del Pilar. Alteraciones moleculares relacionadas con el carcinoma medular de tiroides: estudio mediante inmunohistoquímica y tissue-microarrays.

Degree: Departament de Ciències Mèdiques Bàsiques, 2014, Universitat de Lleida

Medullary Thyroid Carcinoma (MTC) is a relatively rare malignant tumour with C-cell differentiation. 50% of sporadic MTC cases do not exhibit RET mutations. Sprouty is a RET antagonist. Thyroid Paraganglioma (TP) is one of the tumours associated with differential diagnosis. Alterations to several of the genes involved in MTC play an important role in tumoural progression and differential diagnosis. Three MTC-related studies using immunohistochemistry and tissue-arrays were conducted. One of these showed that NF-kB proteins are frequently active in MTC, as are the target genes FLIP and BcL-xL. In a second, a group of young adult mice with selectively deleted spry1 developed C-cell hyperplasia. Following the third, we propose the mandatory use of an immunohistochemical battery of 7 proteins for differential diagnosis between MTC and neck and head paragangliomas, conducting reliability tests to check their reliability on 4 TP cases Advisors/Committee Members: false (authoremailshow), Matias-Guiu, Xavier (director), Encinas Martín, Mario (director), false (authorsendemail).

Subjects/Keywords: Carcinoma medul·lar de tiroide; NF-kappaB; Spry 1; Diagnòstic diferencial; Carcinoma medular de tiroides; Diagnóstico diferencial; Medullary thyroid carcinoma; Differential diagnosis; Anatomia patològica; 611

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Gallel Vicente, M. d. P. (2014). Alteraciones moleculares relacionadas con el carcinoma medular de tiroides: estudio mediante inmunohistoquímica y tissue-microarrays. (Thesis). Universitat de Lleida. Retrieved from http://hdl.handle.net/10803/306437

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Gallel Vicente, María del Pilar. “Alteraciones moleculares relacionadas con el carcinoma medular de tiroides: estudio mediante inmunohistoquímica y tissue-microarrays.” 2014. Thesis, Universitat de Lleida. Accessed May 26, 2019. http://hdl.handle.net/10803/306437.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Gallel Vicente, María del Pilar. “Alteraciones moleculares relacionadas con el carcinoma medular de tiroides: estudio mediante inmunohistoquímica y tissue-microarrays.” 2014. Web. 26 May 2019.

Vancouver:

Gallel Vicente MdP. Alteraciones moleculares relacionadas con el carcinoma medular de tiroides: estudio mediante inmunohistoquímica y tissue-microarrays. [Internet] [Thesis]. Universitat de Lleida; 2014. [cited 2019 May 26]. Available from: http://hdl.handle.net/10803/306437.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gallel Vicente MdP. Alteraciones moleculares relacionadas con el carcinoma medular de tiroides: estudio mediante inmunohistoquímica y tissue-microarrays. [Thesis]. Universitat de Lleida; 2014. Available from: http://hdl.handle.net/10803/306437

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.