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You searched for subject:(5PTase13). Showing records 1 – 2 of 2 total matches.

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Technical University of Lisbon

1. Nunes, Vera Alexandra Sacramento. Functional insights into the plant-specific SR45 splicing factor.

Degree: 2014, Technical University of Lisbon

Mestrado em Engenharia Agronómica - Instituto Superior de Agronomia

Alternative splicing is a post-transcriptional regulatory mechanism that generates transcriptome and proteome diversity. The SR protein family of splicing regulators is essential for the execution and regulation of both constitutive and alternative pre-mRNA splicing. The SR-related protein SR45 is an Arabidopsis bona fide splicing factor that plays a key role in plant development as well as in sugar and abscisic acid (ABA) signaling during early seedling development. The Arabidopsis 5PTase13, belonging to a group of inositol phosphatases involved in plant growth, development and stress responses, is a splicing target of SR45 previously implicated in hormonal and light signaling pathways. The 5PTase13 gene is alternatively spliced, generating two isoforms shown here to be ubiquitously expressed in Arabidopsis. The 5PTase13 loss-of-function mutant we isolated exhibits shorter roots when grown under low light conditions. Moreover, overexpression of either the 5PTase13.1 or the 5PTase13.2 isoforms in the sr45-1 background enhances the glucose hypersensitivity during early seedling development conferred by the sr45-1 mutation. Finally, confocal microscopy data suggests that both 5PTase13 isoforms are located in the cytoplasm, with 5PTase13.1 appearing mostly associated with the plasma membrane. This work provides new insight into the in vivo roles of the SR45 splicing factor and its functional connections to the 5PTase13 phosphatase

Advisors/Committee Members: Santos, Paula Duque Magalhães, Carvalho, Raquel Fonseca, Amâncio, Sara Barros Queiróz.

Subjects/Keywords: 5PTase13; ABA; alternative splicing; Arabidopsis; glucose; SR45

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Nunes, V. A. S. (2014). Functional insights into the plant-specific SR45 splicing factor. (Thesis). Technical University of Lisbon. Retrieved from https://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/8260

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Nunes, Vera Alexandra Sacramento. “Functional insights into the plant-specific SR45 splicing factor.” 2014. Thesis, Technical University of Lisbon. Accessed March 19, 2019. https://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/8260.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Nunes, Vera Alexandra Sacramento. “Functional insights into the plant-specific SR45 splicing factor.” 2014. Web. 19 Mar 2019.

Vancouver:

Nunes VAS. Functional insights into the plant-specific SR45 splicing factor. [Internet] [Thesis]. Technical University of Lisbon; 2014. [cited 2019 Mar 19]. Available from: https://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/8260.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nunes VAS. Functional insights into the plant-specific SR45 splicing factor. [Thesis]. Technical University of Lisbon; 2014. Available from: https://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/8260

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. Sedivy, Eric. Molecular characterization of signaling mechanisms in flowering transition in Arabidopsis and identification of novel flowering QTL in soybean.

Degree: MS, 0030, 2013, University of Illinois – Urbana-Champaign

Flowering response to seasonal photoperiod changes is a critical trait to environmental adaptation and productivity of plants. To better understand flowering two approaches are developed. The first is the molecular characterization of signaling mechanisms in flowering transition in Arabidopsis with the intent to explore basic flowering mechanisms in a model plant species. To this end TERMINAL FLOWER 1 (TFL1), a flowering repressor that controls the activity of the shoot apical meristem (SAM), was selected. Through yeast two-hybrid, we identified nine TFL1 interactors and named them TFL1-IN-LOVE (TIL). Of the nine, TIL3, showed specific binding to TFL1 at a unique TFL1 residue. For this reason TIL3, which encodes an inositol 5-phosphatase (5PTase), was selected for further observation. A functional link between TFL1 and TIL3/5PTase13 was established through root gravitropism experiments, and supported through flowering time experiments where til3-1/5ptase13-1 mutants were shown to reduce the late-flowering effect of TFL1 over-expression. Furthermore, in vivo sub-cellular localization in Nicotiana benthamiana reveals both TFL1 and TIL3/5PTase13 proteins to co-localize in the nucleus and cytoplasm. Finally, Bimolecular Fluorescent Complementation (BiFC) demonstrates in vivo protein-protein interaction between TFL1 and TIL3/5PTase13, supporting our original yeast two-hybrid screen, and reinforcing the hypothesis that TIL3/5PTase13 is important for proper TFL1 function. The second approach devised to understanding flowering is the identification of novel flowering QTLs in Glycine max, the purpose of which is to expand basic knowledge of flowering gained in model species, and apply that knowledge to a cultivate species. In soybean, we conducted QTL mapping using a population of 115 BC2F6 recombinant inbred lines (RILs) that were created from a cross between cultivated soybean, G. max, and its ancestor, G. soja. Agriculturally important traits: flowering time (R1), maturity time (R8), height, yield, lodging, and stem vining were measured for two years in four field locations. QTL mapping analysis identified many previously unidentified QTLs, including 6 independent flowering QTLs with candidate genes, 6 independent maturity QTLs with candidate genes, and 14 QTLs that are observed to affect multiple traits, 3 of which are highly significant. Advisors/Committee Members: Hanzawa, Yoshie (advisor).

Subjects/Keywords: Arabidopsis; Flowering; TERMINAL FLOWER 1 (TFL1); Glycine max; 5PTASE13; Glycine soja

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Sedivy, E. (2013). Molecular characterization of signaling mechanisms in flowering transition in Arabidopsis and identification of novel flowering QTL in soybean. (Thesis). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/45308

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Sedivy, Eric. “Molecular characterization of signaling mechanisms in flowering transition in Arabidopsis and identification of novel flowering QTL in soybean.” 2013. Thesis, University of Illinois – Urbana-Champaign. Accessed March 19, 2019. http://hdl.handle.net/2142/45308.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Sedivy, Eric. “Molecular characterization of signaling mechanisms in flowering transition in Arabidopsis and identification of novel flowering QTL in soybean.” 2013. Web. 19 Mar 2019.

Vancouver:

Sedivy E. Molecular characterization of signaling mechanisms in flowering transition in Arabidopsis and identification of novel flowering QTL in soybean. [Internet] [Thesis]. University of Illinois – Urbana-Champaign; 2013. [cited 2019 Mar 19]. Available from: http://hdl.handle.net/2142/45308.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sedivy E. Molecular characterization of signaling mechanisms in flowering transition in Arabidopsis and identification of novel flowering QTL in soybean. [Thesis]. University of Illinois – Urbana-Champaign; 2013. Available from: http://hdl.handle.net/2142/45308

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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