subject:(0601 Biochemistry AND Cell Biology)

Victoria University

1. Roy, Anna. The EffectsO-1602, O-1918 or Different Dietary Fatty Acids have on Whole body and Skeletal Muscle Energy Homeostasis:A Focus on Putative Cannabinoid Receptors,Adiponectin and Fatty Acid Signalling.

Degree: phd, College of Health & Biomedicine, 2017, Victoria University

URL: http://vuir.vu.edu.au/40256/

► The prevalence and incidence of obesity and related co-morbidities such as insulin resistance and type two diabetes mellitus (T2DM) are rapidly increasing world-wide. These health… (more)

▼ The prevalence and incidence of obesity and related co-morbidities such as insulin resistance and type two diabetes mellitus (T2DM) are rapidly increasing world-wide. These health burdens negatively impact individuals, families, the community and the government, for a multitude of reasons. The search for suitable therapeutic targets for obesity, in combination with a healthy diet and increased physical activity is a strategy for weight management and associated co-morbidities such as T2DM. The endocannabinoid system is a lipid derived signalling system that is modulated by different dietary fatty acids and has a role in regulating energy homeostasis. Modulation of cannabinoid receptor 1 (CB1) reduces whole body adiposity and increases oxidative capacity within the skeletal muscle. The skeletal muscle is a major contributor to whole body energy metabolism through the oxidation of fatty acids, insulin signalling and glucose uptake, therefore understanding the impact that endocannabinoid pharmaceutics have on this tissue is essential. Putative endocannabinoid receptors including G Protein- Coupled Receptor (GPR55) and G Protein-Coupled Receptor 18 (GPR18) may potentially be beneficial pharmaceutical targets for obesity and associated co-morbidities. GPR55 expression is upregulated in visceral adipose tissue in obesity and T2DM. Surprisingly GPR55 knockout mice have increased adiposity and reduced physical activity. While GPR18 has been shown to be expressed in adipose tissue, its role in obesity is unknown. Furthermore the role that atypical cannabinoid compounds, O-1602 or O-1918 (which have/ are hypothesised to have affinities for these receptors and therefore modulate these putative cannabinoid receptors) have in obesity and skeletal muscle homeostasis, following chronic treatment has yet to be determined. The dietary intake of specific fatty acids can also alter circulating endocannabinoid concentrations depending on the type of fatty acid consumed. The effect that different dietary fatty acids have on the putative cannabinoid receptors GPR55 and GPR18 and whole body energy homeostasis in obesity is unknown. Therefore the overall focus for this thesis was to determine the role that atypical cannabinoids have on homeostatic skeletal muscle signalling and whole body energy metabolism in obesity. In addition to this, the effect that different dietary fatty acids have in obesity and whole body energy metabolism were also determined, which may partially be attributed to GPR55 and GPR18 signalling.

Subjects/Keywords: 0601 Biochemistry and Cell Biology; College of Health and Biomedicine

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APA (6^th Edition):

Roy, A. (2017). The EffectsO-1602, O-1918 or Different Dietary Fatty Acids have on Whole body and Skeletal Muscle Energy Homeostasis:A Focus on Putative Cannabinoid Receptors,Adiponectin and Fatty Acid Signalling. (Doctoral Dissertation). Victoria University. Retrieved from http://vuir.vu.edu.au/40256/

Chicago Manual of Style (16^th Edition):

Roy, Anna. “The EffectsO-1602, O-1918 or Different Dietary Fatty Acids have on Whole body and Skeletal Muscle Energy Homeostasis:A Focus on Putative Cannabinoid Receptors,Adiponectin and Fatty Acid Signalling.” 2017. Doctoral Dissertation, Victoria University. Accessed January 17, 2021. http://vuir.vu.edu.au/40256/.

MLA Handbook (7^th Edition):

Roy, Anna. “The EffectsO-1602, O-1918 or Different Dietary Fatty Acids have on Whole body and Skeletal Muscle Energy Homeostasis:A Focus on Putative Cannabinoid Receptors,Adiponectin and Fatty Acid Signalling.” 2017. Web. 17 Jan 2021.

Vancouver:

Roy A. The EffectsO-1602, O-1918 or Different Dietary Fatty Acids have on Whole body and Skeletal Muscle Energy Homeostasis:A Focus on Putative Cannabinoid Receptors,Adiponectin and Fatty Acid Signalling. [Internet] [Doctoral dissertation]. Victoria University; 2017. [cited 2021 Jan 17]. Available from: http://vuir.vu.edu.au/40256/.

Council of Science Editors:

Roy A. The EffectsO-1602, O-1918 or Different Dietary Fatty Acids have on Whole body and Skeletal Muscle Energy Homeostasis:A Focus on Putative Cannabinoid Receptors,Adiponectin and Fatty Acid Signalling. [Doctoral Dissertation]. Victoria University; 2017. Available from: http://vuir.vu.edu.au/40256/

University of Queensland

2. Sullivan, Andrea Michelle. A study of the epidemiology and pathogenicity of salmonella virchow.

Degree: School of Molecular & Microbial Sciences, 1997, University of Queensland

URL: https://espace.library.uq.edu.au/view/UQ:af4eed0/THE12179.pdf ; https://espace.library.uq.edu.au/view/UQ:af4eed0

Subjects/Keywords: 0601 Biochemistry and Cell Biology

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APA (6^th Edition):

Sullivan, A. M. (1997). A study of the epidemiology and pathogenicity of salmonella virchow. (Thesis). University of Queensland. Retrieved from https://espace.library.uq.edu.au/view/UQ:af4eed0/THE12179.pdf ; https://espace.library.uq.edu.au/view/UQ:af4eed0

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sullivan, Andrea Michelle. “A study of the epidemiology and pathogenicity of salmonella virchow.” 1997. Thesis, University of Queensland. Accessed January 17, 2021. https://espace.library.uq.edu.au/view/UQ:af4eed0/THE12179.pdf ; https://espace.library.uq.edu.au/view/UQ:af4eed0.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sullivan, Andrea Michelle. “A study of the epidemiology and pathogenicity of salmonella virchow.” 1997. Web. 17 Jan 2021.

Vancouver:

Sullivan AM. A study of the epidemiology and pathogenicity of salmonella virchow. [Internet] [Thesis]. University of Queensland; 1997. [cited 2021 Jan 17]. Available from: https://espace.library.uq.edu.au/view/UQ:af4eed0/THE12179.pdf ; https://espace.library.uq.edu.au/view/UQ:af4eed0.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sullivan AM. A study of the epidemiology and pathogenicity of salmonella virchow. [Thesis]. University of Queensland; 1997. Available from: https://espace.library.uq.edu.au/view/UQ:af4eed0/THE12179.pdf ; https://espace.library.uq.edu.au/view/UQ:af4eed0

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Queensland

3. Chesterfield, Rebecca. A synthetic biology toolbox for examining and engineering strigolactone biosynthesis.

Degree: Australian Institute for Bioengineering and Nanotechnology, 2020, University of Queensland

URL: https://espace.library.uq.edu.au/view/UQ:ad47224/thumbnail_s4417014_final_thesis_t.jpg ; https://espace.library.uq.edu.au/view/UQ:ad47224/s4417014_final_thesis.pdf ; https://espace.library.uq.edu.au/view/UQ:ad47224

► Strigolactones (SLs) are plant hormones and rhizosphere-signalling molecules that control plant architecture and environmental adaption, promote symbioses with soil organisms, and mediate root parasitism. These… (more)

▼ Strigolactones (SLs) are plant hormones and rhizosphere-signalling molecules that control plant architecture and environmental adaption, promote symbioses with soil organisms, and mediate root parasitism. These diverse activities give SLs great promise as agrochemicals, with potential applications in optimising plant architecture, controlling parasitic weeds, enhancing nutrient uptake, and improving tolerance to drought and salinity. However, agricultural use is currently unfeasible as there are no economically viable sources of SLs or SL analogues. Microbes engineered for SL biosynthesis could provide a cheap, renewable, and scalable production method that could overcome current supply challenges, and enable SLs to be deployed as agrochemicals.Synthetic biology – characterised by modularity, standardisation, interoperability of biological parts, and engineering principals such as the design-build-test-learn cycle – offers an approach to transform microbial engineering into an engineering discipline. This thesis describes the development of a synthetic biology toolbox for studying and engineering SL biosynthesis, comprising a SL production module, a detection module, and characterisation of one of the least understood enzymes in the pathway. Saccharomyces cerevisiae was selected as the initial host organism due to its favourable industrial properties, and previous work demonstrating that it is a suitable host for multiple elements in the SL biosynthetic pathway. However, conversion of β-carotene to CL was achieved at very low titres, with CL produced at approximately 1,000,000-fold lower concentration than b-carotene. This was at least in part due to poor conversion of all-trans-β-carotene to 9-cis-β-carotene through DWARF27 (D27), prompting further investigation into this enzyme.Characterization of enzymes increases the reliability of their deployment as a biological part in an engineered system, and provides background information for enzyme engineering. As little is known about the D27 structure-function relationship, the localization and activity of rice (Oryza sativa) D27 (OsD27) were investigated, and efforts were made to elucidate its three-dimensional structure. In a transient Nicotiana benthamiana system, OsD27 was found in stromal and thylakoid membrane-bound forms, demonstrating the sub-organellar localisation of this enzyme for the first time. A maltose binding protein (MBP) fusion of OsD27 had activity in E. coli, and the purified fusion of OsD27 catalysed the reversible isomerisation of β-carotene around the C9-C10 double bond in vitro, with Km = 3.3 ± 1.2 mM for all-trans-β-carotene and Km = 5.4 ± 1.4 mM for 9-cis-β-carotene. Extensive efforts to crystallise OsD27 for structural characterization did not yield results. Purified MBP fusions of OsD27 aggregated into soluble oligomers ranging from 10-150 nm in hydrodynamic radius. The aggregates were not compatible with crystal formation, and could not be completely dissociated using detergents. A non-aggregating D27 homologue from Ziziphus jujuba was…

Subjects/Keywords: Strigolactones; Biosynthesis; Biosensor; Metabolic engineering; Synthetic biology; 0601 Biochemistry and Cell Biology; 0607 Plant Biology

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APA (6^th Edition):

Chesterfield, R. (2020). A synthetic biology toolbox for examining and engineering strigolactone biosynthesis. (Thesis). University of Queensland. Retrieved from https://espace.library.uq.edu.au/view/UQ:ad47224/thumbnail_s4417014_final_thesis_t.jpg ; https://espace.library.uq.edu.au/view/UQ:ad47224/s4417014_final_thesis.pdf ; https://espace.library.uq.edu.au/view/UQ:ad47224

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chesterfield, Rebecca. “A synthetic biology toolbox for examining and engineering strigolactone biosynthesis.” 2020. Thesis, University of Queensland. Accessed January 17, 2021. https://espace.library.uq.edu.au/view/UQ:ad47224/thumbnail_s4417014_final_thesis_t.jpg ; https://espace.library.uq.edu.au/view/UQ:ad47224/s4417014_final_thesis.pdf ; https://espace.library.uq.edu.au/view/UQ:ad47224.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chesterfield, Rebecca. “A synthetic biology toolbox for examining and engineering strigolactone biosynthesis.” 2020. Web. 17 Jan 2021.

Vancouver:

Chesterfield R. A synthetic biology toolbox for examining and engineering strigolactone biosynthesis. [Internet] [Thesis]. University of Queensland; 2020. [cited 2021 Jan 17]. Available from: https://espace.library.uq.edu.au/view/UQ:ad47224/thumbnail_s4417014_final_thesis_t.jpg ; https://espace.library.uq.edu.au/view/UQ:ad47224/s4417014_final_thesis.pdf ; https://espace.library.uq.edu.au/view/UQ:ad47224.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chesterfield R. A synthetic biology toolbox for examining and engineering strigolactone biosynthesis. [Thesis]. University of Queensland; 2020. Available from: https://espace.library.uq.edu.au/view/UQ:ad47224/thumbnail_s4417014_final_thesis_t.jpg ; https://espace.library.uq.edu.au/view/UQ:ad47224/s4417014_final_thesis.pdf ; https://espace.library.uq.edu.au/view/UQ:ad47224

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Victoria University

4. Robinson, Ainsley. Stem cell therapies for the treatment of enteric neuropathy associated with inflammatory bowel disease.

Degree: phd, College of Health and Biomedicine, 2019, Victoria University

URL: http://vuir.vu.edu.au/39508/

► Although not associated with mortality, symptoms, complications and the relapsing nature of inflammatory bowel disease (IBD) severely impact patient’s quality of life. Current treatments are… (more)

▼ Although not associated with mortality, symptoms, complications and the relapsing nature of inflammatory bowel disease (IBD) severely impact patient’s quality of life. Current treatments are coupled with side effects and loss of patient response. Damage to the enteric neurons is consistently associated with intestinal inflammation and considered to underlie the generation of symptoms. Therefore, the enteric neurons are a potential target for novel IBD therapies. Mesenchymal stem cells (MSCs) exhibit anti-inflammatory, immunomodulating, and neuroprotective effects and are demonstrated to participate in tissue regeneration and repair in many pathological conditions. Hence, they are a viable option for the treatment of enteric neuropathy associated with IBD. The studies in this thesis aim to investigate the effects of MSC therapy in averting enteric neuropathy in acute and chronic models of IBD. The results of our studies demonstrated that MSC and conditioned medium attenuated inflammation and averted enteric neuropathy and colonic dysmotility in an acute model of IBD. The effects of MSC treatment are dose-dependent and occur as early as 24h post treatment. We characterized changes to colonic innervation, motility, transit time, microbiota and metabolome in the Winnie mouse model of spontaneously occurring chronic colitis. Our results demonstrated that the Winnie mouse is highly representative of human IBD. The mechanisms underlying colonic dysmotility in Winnie mice were due to inhibition of neuromuscular transmission and smooth muscle responses. We found that multiple high dose MSC treatments induce anti-inflammatory and neurotrophic effects in mice with chronic colitis. Single dose and multiple low dose MSC administrations were ineffective in this model. Overall, we have established the capacity of MSC treatments to attenuate inflammation and enteric neuropathy in acute and chronic models of IBD. These findings are both novel and highly relevant for clinical translation and future investigations of MSC therapy for the treatment of IBD.

Subjects/Keywords: 0601 Biochemistry and Cell Biology; 1103 Clinical Sciences; 1107 Immunology; College of Health and Biomedicine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Robinson, A. (2019). Stem cell therapies for the treatment of enteric neuropathy associated with inflammatory bowel disease. (Doctoral Dissertation). Victoria University. Retrieved from http://vuir.vu.edu.au/39508/

Chicago Manual of Style (16^th Edition):

Robinson, Ainsley. “Stem cell therapies for the treatment of enteric neuropathy associated with inflammatory bowel disease.” 2019. Doctoral Dissertation, Victoria University. Accessed January 17, 2021. http://vuir.vu.edu.au/39508/.

MLA Handbook (7^th Edition):

Robinson, Ainsley. “Stem cell therapies for the treatment of enteric neuropathy associated with inflammatory bowel disease.” 2019. Web. 17 Jan 2021.

Vancouver:

Robinson A. Stem cell therapies for the treatment of enteric neuropathy associated with inflammatory bowel disease. [Internet] [Doctoral dissertation]. Victoria University; 2019. [cited 2021 Jan 17]. Available from: http://vuir.vu.edu.au/39508/.

Council of Science Editors:

Robinson A. Stem cell therapies for the treatment of enteric neuropathy associated with inflammatory bowel disease. [Doctoral Dissertation]. Victoria University; 2019. Available from: http://vuir.vu.edu.au/39508/

University of Queensland

5. Mutemi, Kevin Nzumbi. Investigating the role of Dscam2 in the Drosophila mushroom bodies.

Degree: School of Medicine, 2018, University of Queensland

URL: https://espace.library.uq.edu.au/view/UQ:c5233ae/s4262593_masters_thesis.pdf ; https://espace.library.uq.edu.au/view/UQ:c5233ae

► This thesis is an attempt at investigating the role that Drosophila melanogaster(hereafter referred to as Drosophila) Down syndrome cell adhesion molecule 2(Dscam2) plays in mushroom… (more)

Subjects/Keywords: Drosophila; Dscam2; Dendrite; Development; Mushroom bodies; Localisation; Isoforms; 0601 Biochemistry and Cell Biology; 0604 Genetics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mutemi, K. N. (2018). Investigating the role of Dscam2 in the Drosophila mushroom bodies. (Thesis). University of Queensland. Retrieved from https://espace.library.uq.edu.au/view/UQ:c5233ae/s4262593_masters_thesis.pdf ; https://espace.library.uq.edu.au/view/UQ:c5233ae

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mutemi, Kevin Nzumbi. “Investigating the role of Dscam2 in the Drosophila mushroom bodies.” 2018. Thesis, University of Queensland. Accessed January 17, 2021. https://espace.library.uq.edu.au/view/UQ:c5233ae/s4262593_masters_thesis.pdf ; https://espace.library.uq.edu.au/view/UQ:c5233ae.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mutemi, Kevin Nzumbi. “Investigating the role of Dscam2 in the Drosophila mushroom bodies.” 2018. Web. 17 Jan 2021.

Vancouver:

Mutemi KN. Investigating the role of Dscam2 in the Drosophila mushroom bodies. [Internet] [Thesis]. University of Queensland; 2018. [cited 2021 Jan 17]. Available from: https://espace.library.uq.edu.au/view/UQ:c5233ae/s4262593_masters_thesis.pdf ; https://espace.library.uq.edu.au/view/UQ:c5233ae.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mutemi KN. Investigating the role of Dscam2 in the Drosophila mushroom bodies. [Thesis]. University of Queensland; 2018. Available from: https://espace.library.uq.edu.au/view/UQ:c5233ae/s4262593_masters_thesis.pdf ; https://espace.library.uq.edu.au/view/UQ:c5233ae

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Victoria University

6. Smith, Renee Melissa. The Role Of Putative Nox Inhibitors In Homocysteine-Induced Vascular Dysfunction.

Degree: phd, Institute for Health and Sport, 2018, Victoria University

URL: http://vuir.vu.edu.au/39496/

► Excess plasma homocysteine (Hcy; hyperhomocysteinemia, HHcy) remains an independent risk factor for cardiovascular disease (CVD) and treatments remain elusive. The source of Hcy, methionine, is… (more)

▼ Excess plasma homocysteine (Hcy; hyperhomocysteinemia, HHcy) remains an independent risk factor for cardiovascular disease (CVD) and treatments remain elusive. The source of Hcy, methionine, is an essential amino acid acquired by ingestion of animal foods. Normal methionine metabolism effectively removes Hcy via recycling back into methionine or excretion via the kidney. However, in aberrant methionine metabolism, Hcy accumulates and causes damage to the vascular system by increasing oxidative stress; the exact mechanism of how this occurs is unknown. Importantly, the B vitamins B6, B9 and B12 are essential to proper methionine/Hcy metabolism and are often found in low levels in patients presenting with HHcy; this has provided a potentially viable treatment strategy in the clinical setting. Disappointingly, clinical trials administering B vitamins to reduce HHcy have been unsuccessful in reducing CVD and treatments continue to be sought. The NADPH oxidase (Nox) family of enzymes are expressed in a broad range of cell types throughout the body and are the primary source of superoxide (Nox1, Nox2) and hydrogen peroxide (Nox4) within the vasculature under both physiological and pathological conditions. Nox1, 2 and 4 are of primary interest in vascular disease, as there is evidence that Hcy can interfere with the proper function of Nox1, 2 and 4 signalling, potentially leading to an over-expression of pro-oxidants. Nox1, 2 and 4 have been implicated in vascular disease (endothelial dysfunction), hypertension, vascular inflammation, stroke, diabetes, and atherosclerosis, and putative inhibitors of these enzymes are now available. Additionally, nitric oxide (NO) is also measured as a marker of proper vascular function; indeed, the current gold standard of assessing NO availability is by indirectly measuring vascular responses to acetylcholine. Accordingly, a loss of NO bioavailability is linked to the development of many of the same vascular pathologies caused by HHcy and also potentially increased Nox1, 2, and 4 activity. Thus, this thesis examined if current putative Nox inhibitors could prevent vascular dysfunction caused by homocysteine (as indirectly measured by acetylcholine-mediated vasorelaxation). Using New Zealand white rabbits, C57BL/6 mice and a Nox2-/- (C57BL/6 background) mouse models, we observed that pharmacological intervention with single Nox1, 2 and 4 inhibitors reduced the effect of acetylcholine on vasorelaxation. In 1% methionine-fed Nox2-/- mice, we observed an improvement in function. We also assessed combinations of Nox1, 2 and 4 inhibitors and found that, although function was not restored to control levels, it was improved compared with single Nox inhibition. Due to these results, we performed a gp91ds-tat dose response in rabbit aorta. We found that in our models of vascular dysfunction, lower doses of gp91ds-tat significantly improved acetylcholine-mediated vasorelaxation. These results showed for the first time that in both pharmacological and diet-induced HHcy, high dose putative Nox…

Subjects/Keywords: 0601 Biochemistry and Cell Biology; 1101 Medical Biochemistry and Metabolomics; 1102 Cardiorespiratory Medicine and Haematology; Institute for Health and Sport

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Smith, R. M. (2018). The Role Of Putative Nox Inhibitors In Homocysteine-Induced Vascular Dysfunction. (Doctoral Dissertation). Victoria University. Retrieved from http://vuir.vu.edu.au/39496/

Chicago Manual of Style (16^th Edition):

Smith, Renee Melissa. “The Role Of Putative Nox Inhibitors In Homocysteine-Induced Vascular Dysfunction.” 2018. Doctoral Dissertation, Victoria University. Accessed January 17, 2021. http://vuir.vu.edu.au/39496/.

MLA Handbook (7^th Edition):

Smith, Renee Melissa. “The Role Of Putative Nox Inhibitors In Homocysteine-Induced Vascular Dysfunction.” 2018. Web. 17 Jan 2021.

Vancouver:

Smith RM. The Role Of Putative Nox Inhibitors In Homocysteine-Induced Vascular Dysfunction. [Internet] [Doctoral dissertation]. Victoria University; 2018. [cited 2021 Jan 17]. Available from: http://vuir.vu.edu.au/39496/.

Council of Science Editors:

Smith RM. The Role Of Putative Nox Inhibitors In Homocysteine-Induced Vascular Dysfunction. [Doctoral Dissertation]. Victoria University; 2018. Available from: http://vuir.vu.edu.au/39496/

University of Queensland

7. Bokhari, Fawzi Faisal A. siRNA screening of the kinome identified Aurora A kinase as a therapeutic target gene in cervical cancer.

Degree: UQ Diamantina Institute, 2014, University of Queensland

URL: http://espace.library.uq.edu.au/view/UQ:341649

► HPV oncogenes disable a number of tumour suppressor pathways, including p53 and Rb, contributing to the transformed phenotype. Loss of these critical host cell functions… (more)

▼ HPV oncogenes disable a number of tumour suppressor pathways, including p53 and Rb, contributing to the transformed phenotype. Loss of these critical host cell functions may also provide an opportunity to selectively target the destruction of HPV-transformed cells. We have performed an siRNA screen using the kinome (779 genes) library to identify genes that when depleted are synthetically lethal with HPV transformation. The primary and validations screens have confirmed Aurora A kinase (AURKA) as a potential synthetic lethal target selective for HPV transformed cells. AURKA has been further investigated using the selective small molecule inhibitor MLN8237. We found that MLN8237 was significantly more potent towards the HPV transformed cells. The effect was not a consequence of targeting mitosis as two other mitotic inhibitors, PLK1 inhibitor (BI2536) and taxol, demonstrated no selectivity. Analysis of the nuclear structure and DNA content showed that Aurora A inhibition promoted a high level of polyploidy in non-HPV treated cells whilst this same degree of polyploidy was associate with apoptosis in the HPV-transformed cell lines. Whereas Bcl-2 over expression in HeLa cells had no effect on sensitivity to MLN8237, Mcl-1 overexpressing HeLa cells were less sensitive to the MLN8237 in comparison to the parental cell line, which may suggests the involvement of Noxa or Puma pro-apoptotic proteins in the induction of the apoptosis in the HPV-transformed cells. The transfection of the non-HPV C33A cervical cancer and SCC25 squamous cell carcinoma cell lines with the HPV16 oncogenic E7 increased sensitivity to MLN8237 between 3 >10 fold suggesting that the sensitivity to MLN8237-dependent killing was a direct consequence of HPV E7 expression. Xenograft experiments with cervical cancer cell lines in immunodeficient mice showed MLN8237 inhibited growth of HPV and non-HPV xenografts during treatment with 30mg/kg MLN8237 once a day for 10 consecutive days. However, outgrowth of tumour was noticed from the second day post-treatment in the non-HPV tumour group whereas the HPV-induced tumour group did not show cancer recurrence for 50 days post-treatment. These findings suggest that MLN8237 represent a promising novel therapeutic targeted agent against HPV-transformed cervical cancer.

Subjects/Keywords: siRNA; AURKA; AURKB; Haspin; GSG2; cervical cancer; HPV; synthetic lethality; 0601 Biochemistry and Cell Biology; 0604 Genetics; 1112 Oncology and Carcinogenesis

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APA (6^th Edition):

Bokhari, F. F. A. (2014). siRNA screening of the kinome identified Aurora A kinase as a therapeutic target gene in cervical cancer. (Thesis). University of Queensland. Retrieved from http://espace.library.uq.edu.au/view/UQ:341649

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bokhari, Fawzi Faisal A. “siRNA screening of the kinome identified Aurora A kinase as a therapeutic target gene in cervical cancer.” 2014. Thesis, University of Queensland. Accessed January 17, 2021. http://espace.library.uq.edu.au/view/UQ:341649.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bokhari, Fawzi Faisal A. “siRNA screening of the kinome identified Aurora A kinase as a therapeutic target gene in cervical cancer.” 2014. Web. 17 Jan 2021.

Vancouver:

Bokhari FFA. siRNA screening of the kinome identified Aurora A kinase as a therapeutic target gene in cervical cancer. [Internet] [Thesis]. University of Queensland; 2014. [cited 2021 Jan 17]. Available from: http://espace.library.uq.edu.au/view/UQ:341649.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bokhari FFA. siRNA screening of the kinome identified Aurora A kinase as a therapeutic target gene in cervical cancer. [Thesis]. University of Queensland; 2014. Available from: http://espace.library.uq.edu.au/view/UQ:341649

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Queensland

8. Sullivan, Mitchell. Liver-glycogen metabolism: A structural perspective.

Degree: Queensland Alliance for Agricultural and Food Innovation, 2014, University of Queensland

URL: http://espace.library.uq.edu.au/view/UQ:347324

► Liver glycogen, a highly branched glucose polymer, has a critical role in the maintenance of blood glucose homeostasis. Liver glycogen consists of glucose units that… (more)

▼ Liver glycogen, a highly branched glucose polymer, has a critical role in the maintenance of blood glucose homeostasis. Liver glycogen consists of glucose units that are attached to form linear chains via alpha-(1→4) linkages. These chains are connected via alpha-(1→6)-linked branch points to form highly branched glycogen “beta” particles (~20 nm in diameter) that can further join to form much larger “alpha” particles (~100-200 nm). Given the characteristically poor blood-glucose control associated with type 2 diabetes, a link between the structure/function relationships of liver glycogen and type 2 diabetes is probable. It is shown that diabetic (db/db) mice have an impaired ability to synthesize the large composite glycogen alpha particles present in normal, healthy mice and that alpha particles are held together via a bond more acid labile than normal glycosidic linkages, with the most likely bond being proteinaceous. The structure of healthy mouse liver glycogen over the diurnal cycle is characterized using size exclusion chromatography and transmission electron microscopy. Glycogen is observed to be initially formed as smaller beta particles, only being assembled into the larger alpha particles significantly after the time when glycogen content reaches a maximum. This pathway, impaired in diabetic animals, is likely to give optimal blood-glucose control, as explained by the particles’ surface area to volume ratio. Lack of this control may result from, or contribute to, the poor glycaemic regulation associated with diabetes. This discovery suggests novel approaches to diabetes management that promote alpha particle formation. Significant improvements in the extraction and characterization of liver glycogen has also been achieved, paving the way for future experiments exploring glycogen’s role in diabetes. Glycogen can now be effectively and rapidly extracted from formalin-fixed tissues using a novel technique, allowing the analysis of human tissue samples from pathology laboratories that routinely employ this method of fixation. The use of aqueous size exclusion chromatography has been shown to dramatically improve peak resolution when compared to the previously used dimethyl sulfoxide method, achieving separation of alpha-particle and beta-particle peaks. This allows for a more detailed and quantitative analysis and comparison between liver glycogen samples.

Subjects/Keywords: Glycogen; Size exclusion chromatography; Glycaemic control; Structural characterization; Diabetes; Alpha particle; 0306 Physical Chemistry (incl. Structural); 0601 Biochemistry and Cell Biology

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APA (6^th Edition):

Sullivan, M. (2014). Liver-glycogen metabolism: A structural perspective. (Thesis). University of Queensland. Retrieved from http://espace.library.uq.edu.au/view/UQ:347324

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sullivan, Mitchell. “Liver-glycogen metabolism: A structural perspective.” 2014. Thesis, University of Queensland. Accessed January 17, 2021. http://espace.library.uq.edu.au/view/UQ:347324.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sullivan, Mitchell. “Liver-glycogen metabolism: A structural perspective.” 2014. Web. 17 Jan 2021.

Vancouver:

Sullivan M. Liver-glycogen metabolism: A structural perspective. [Internet] [Thesis]. University of Queensland; 2014. [cited 2021 Jan 17]. Available from: http://espace.library.uq.edu.au/view/UQ:347324.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sullivan M. Liver-glycogen metabolism: A structural perspective. [Thesis]. University of Queensland; 2014. Available from: http://espace.library.uq.edu.au/view/UQ:347324

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Queensland

9. Iljas, Juvita Delancy. The in vivo roles of Sirtuin 1 and 3 in age and obesity-related decline in female fertility.

Degree: Faculty of Medicine, 2020, University of Queensland

URL: http://espace.library.uq.edu.au/view/UQ:2

► Sirtuin 1 (Sirt1) and Sirtuin 3 (Sirt3), are members of the sirtuin family of NAD+-dependent deacetylases. Sirt1 exerts its function in the nucleus, whereas Sirt3… (more)

▼ Sirtuin 1 (Sirt1) and Sirtuin 3 (Sirt3), are members of the sirtuin family of NAD+-dependent deacetylases. Sirt1 exerts its function in the nucleus, whereas Sirt3 exerts its function in mitochondria. Both Sirt1 and Sirt3 are indispensable for mitochondrial function and antioxidant defences in somatic cells. Mitochondria and oxidative stress are pivotal determinants of oocyte developmental competence (also known as quality). Thus far, analyses of Sirt1 and Sirt3 activities in oocytes have been limited to in vitro knockdown and chemical inhibitor approaches. Significantly, the in vivo roles of Sirt1 and Sirt3 for female fertility have not been formally tested. Since results with whole-animal knockouts would be confounded by the indispensable requirement for Sirt1 in somatic cells, I used the Zp3-Cre-LoxP system to specifically delete Sirt1’s catalytic domain in vivo in growing oocytes (OoSirt1∆Ex4/∆Ex4). To investigate the role of Sirt3 for female fertility, I employed a whole-animal Sirt3-knockout (Sirt3-/-) mouse model.I evaluated female reproductive capacity by quantifying ovarian follicles (ovarian sections); in vitro meiotic maturation, spindle assembly and chromosome segregation (time-lapse confocal microscopy); in vitro preimplantation embryo development; expression of SIRT1 and SIRT3 transcription targets (qRT-PCR); oocyte oxidative stress and mitochondrial content (confocal imaging), and; reproductive capacity (mating trials).Surprisingly, I found that all these parameters were indistinguishable in young (2-month-old) OoSirt1∆Ex4/∆Ex4 and Sirt3-/- females compared to corresponding wild-type females. I reasoned that the roles of Sirt1 deacetylase activity in oocytes (oocyte-Sirt1) and Sirt3 in mitochondrial function and antioxidant defences might become apparent under stressed conditions. Therefore, I undertook further analyses under two in vivo stressed states: natural ageing (10-12-months) and obesity induced by high-fat diet (HFD).Very interestingly, my analysis revealed an age-accelerated decline in reproductive performance in OoSirt1∆Ex4/∆Ex4 females, which was due to a decline in oocyte quality. Interestingly, compromised oocyte quality does not impact in vivo oocyte maturation or fertilisation but leads to increased oxidative stress in preimplantation embryos inhibiting cleavage divisions. Remarkably, HFD-induced obese OoSirt1∆Ex4/∆Ex4 females showed no defects in any of the above parameters (including litter sizes) when compared to wild-type females. Also, Sirt3-/- females exhibit no defects in oocyte quality and reproductive performance even following HFD-induced obesity and natural ageing.In my PhD project, I thoroughly interrogate the in vivo roles of oocyte-Sirt1 and Sirt3 for female fertility for the first time. My data suggest that defects in aged females lacking oocyte-Sirt1 arise due to concurrent age-related changes such as reduced NAD+ and sirtuin expression levels, which may compromise compensatory mechanisms that can cover for Sirt1 loss in younger oocytes. In contrast to evidence that…

Subjects/Keywords: Oocyte; Embryo; Sirt1; Sirt3; Fertility; Obesity; Ageing; Oxidative stress; Mitochondria; DNA damage; 0601 Biochemistry and Cell Biology; 0604 Genetics

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APA (6^th Edition):

Iljas, J. D. (2020). The in vivo roles of Sirtuin 1 and 3 in age and obesity-related decline in female fertility. (Thesis). University of Queensland. Retrieved from http://espace.library.uq.edu.au/view/UQ:2

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Iljas, Juvita Delancy. “The in vivo roles of Sirtuin 1 and 3 in age and obesity-related decline in female fertility.” 2020. Thesis, University of Queensland. Accessed January 17, 2021. http://espace.library.uq.edu.au/view/UQ:2.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Iljas, Juvita Delancy. “The in vivo roles of Sirtuin 1 and 3 in age and obesity-related decline in female fertility.” 2020. Web. 17 Jan 2021.

Vancouver:

Iljas JD. The in vivo roles of Sirtuin 1 and 3 in age and obesity-related decline in female fertility. [Internet] [Thesis]. University of Queensland; 2020. [cited 2021 Jan 17]. Available from: http://espace.library.uq.edu.au/view/UQ:2.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Iljas JD. The in vivo roles of Sirtuin 1 and 3 in age and obesity-related decline in female fertility. [Thesis]. University of Queensland; 2020. Available from: http://espace.library.uq.edu.au/view/UQ:2

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Queensland

10. Perdomo Contreras, Hugo. Interactions between flaviviruses and the vector Aedes aegypti, an overview into replication and microRNAs.

Degree: School of Biological Sciences, 2020, University of Queensland

URL: http://espace.library.uq.edu.au/view/UQ:65

► Mosquitoes are considered as the most dangerous animals on earth if all the diseases they transmit are taken into account. Different species of mosquitoes transmit… (more)

▼ Mosquitoes are considered as the most dangerous animals on earth if all the diseases they transmit are taken into account. Different species of mosquitoes transmit different pathogens ranging from viruses to parasites, but the transmission of these microorganisms comes down to a specific aspect of those mosquitoes’ biology, blood feeding. One of the most important vectors is Aedes aegypti responsible for transmission of viral diseases such as Dengue, Zika and Chikungunya among others. From those, dengue virus (DENV) has the highest prevalence, infecting about 390 million people a year in over 100 countries. No efficient vaccine or treatment exists at the moment making vector control the main way of prevention. Despite the existence of different methods to control the vector, epidemics continue to arise. This makes the study of A. aegypti biology and its interaction with different viruses important in the fight against these diseases.When DENV infects a cell, a reorganization of the membranes of the endoplasmic reticulum forms the replication complex, a place where viral translation and replication occurs. In mammals, the formation of the replication complex is preceded by the binding of viral non-structural proteins to STT3A, a member of the oligosaccharyl transferase complex (OST). Knockout of STT3A decreases viral titres in orders of magnitude in mammalian cells. We found that after silencing STT3A or other proteins of the OST complex, STT3B and MAGT1, viral titres and genomic copies of DENV were not altered in A. aegypti cells. The reason for this is not known but this points to an important difference in the formation of replication complexes in mosquito and mammalian cells.One of the most important biological processes for mosquitoes is blood feeding. This process is key for two main reasons: (1) this is the moment when a mosquito gets infected with a pathogen. In the context of A. aegypti-DENV interaction, the infection, replication and virion production of DENV has been studied extensively in mammalian cell lines, but fundamental aspects of the replication of the virus in mosquitoes are still less known. (2) Blood feeding is the way females acquire nutrients for egg laying, making it a vital step in their life cycle. Blood is a complex mixture of water, cells, proteins, lipids, and other elements that have regulatory functions in mosquito physiology. While the effect of most of these components on mosquitoes have been studied in detail, the potential regulatory role of host blood microRNAs (miRNAs) on mosquitoes has not been investigated.miRNAs are RNA molecules of approximately 22 nucleotides in length that regulate gene expression post-transcriptionally. miRNAs have been shown to modify many biological processes in a wide range of organisms including A. aegypti. We detected human miRNAs (hsa-miRNAs) in the fat body of A. aegypti mosquitoes as early as 6 hours after blood feeding lasting up to 24 hours after blood feeding. We used the two most abundant miRNAs found in the fat body to determine if they have…

Subjects/Keywords: Aedes aegypti; Dengue virus; Micro RNA; Blood feeding; Replication; Extracellular RNA; 0601 Biochemistry and Cell Biology; 0605 Microbiology; 0608 Zoology

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APA (6^th Edition):

Perdomo Contreras, H. (2020). Interactions between flaviviruses and the vector Aedes aegypti, an overview into replication and microRNAs. (Thesis). University of Queensland. Retrieved from http://espace.library.uq.edu.au/view/UQ:65

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Perdomo Contreras, Hugo. “Interactions between flaviviruses and the vector Aedes aegypti, an overview into replication and microRNAs.” 2020. Thesis, University of Queensland. Accessed January 17, 2021. http://espace.library.uq.edu.au/view/UQ:65.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Perdomo Contreras, Hugo. “Interactions between flaviviruses and the vector Aedes aegypti, an overview into replication and microRNAs.” 2020. Web. 17 Jan 2021.

Vancouver:

Perdomo Contreras H. Interactions between flaviviruses and the vector Aedes aegypti, an overview into replication and microRNAs. [Internet] [Thesis]. University of Queensland; 2020. [cited 2021 Jan 17]. Available from: http://espace.library.uq.edu.au/view/UQ:65.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Perdomo Contreras H. Interactions between flaviviruses and the vector Aedes aegypti, an overview into replication and microRNAs. [Thesis]. University of Queensland; 2020. Available from: http://espace.library.uq.edu.au/view/UQ:65

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Queensland

11. Kamli, Hossam. Exploring resistance of renal cell carcinoma to sunitinib therapy.

Degree: Faculty of Medicine, 2020, University of Queensland

URL: http://espace.library.uq.edu.au/view/UQ:3

► Renal cell carcinomas (RCC) are highly metastatic and therapy-resistant. Several novel tyrosine kinase inhibitors (TKI) targeting angiogenesis have been introduced into clinical practice for RCC, the most… (more)

▼ Renal cell carcinomas (RCC) are highly metastatic and therapy-resistant. Several novel tyrosine kinase inhibitors (TKI) targeting angiogenesis have been introduced into clinical practice for RCC, the most common being sunitinib. However, use of sunitinib is limited by development of therapy resistance in RCC. Comprehensive characterisation of morphological, functional and molecular changes in sunitinib-resistant RCC cells is lacking. Understanding the mechanisms of resistance to sunitinib therapy, and developing measures to overcome resistance, may help improve patient care.The overall hypotheses of this project were: 1) that RCC models that demonstrate resistance to current RCC therapies can be utilised to investigate and define molecular pathways of resistance; and 2) that these same models can be used to test novel therapies. Specifically, the project aimed: 1) to develop and characterise RCC cell lines that are resistant to sunitinib; 2) to explore the mechanisms of resistance in the RCC cell lines with emphasis on angiogenic and apoptotic pathways; 3) to overcome sunitinib-resistance with appropriate agents using in vitro methodologies; and 4) to validate in vitro findings in a murine xenograft model of RCC.A literature review is provided in Chapter 1, and general Materials and Methods are described in Chapter 2. Chapters 3 describes development of sunitinib resistance in four human RCC cell lines: 786-0, Caki-1, Caki-2, and SN12K1 and characterise the differences in cell and molecular biology from parental RCC cell lines. The RCC cell lines were made resistant to continuous, chronic exposure to 10 μM sunitinib. Cell growth/proliferation (MTT assay), morphology and apoptosis (phase-contrast microscopy and hematoxylin-eosin staining), transmigration (migration chamber), and gene expression (qRT-PCR) for interleukin-6 and -8 (IL-6, IL-8), vascular endothelial growth factor (VEGF), Bcl2 and Bax were studied. Resistant cells were stable in 10 μM sunitinib, maintained viability in 20 μM sunitinib, and have now been in culture for over two years. Resistant cells proliferated more slowly, but were significantly hypertrophic compared with parental cells. The transmigration assay showed no difference between resistant cells and their respective parental cells. One of the most striking differences between resistant and parental RCC was that IL-6 (in all RCC) and IL-8 (in most RCC) significantly increased in resistant cells, with an associated increase in VEGF. An anti-apoptotic mechanism was also demonstrated in therapy-resistant RCC. Increased IL-6 may contribute to resistance of RCC through downstream upregulation VEGF.Thus, IL-6 increase was considered worthy of further investigation as a potential therapeutic target for therapy-resistant RCC. In Chapter 4, resistant cells were grown with and without Tocilizumab (50 μg/mL), a monoclonal antibody that blocks the IL-6 receptor (IL-6R) protein. Cell growth/proliferation, cell morphology and apoptosis, gene expression (qRT-PCR) and protein levels (ELISA) for IL-6, VEGF,…

Subjects/Keywords: Angiogenesis; Apoptosis; Renal cell carcinoma; Clear cell renal cell carcinoma; Immunotherapy; Inhibitors; Interleukin-6; Kidney cancer; Sunitinib-resistance; Tocilizumab; 0601 Biochemistry and Cell Biology; 1103 Clinical Sciences; 1107 Immunology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kamli, H. (2020). Exploring resistance of renal cell carcinoma to sunitinib therapy. (Thesis). University of Queensland. Retrieved from http://espace.library.uq.edu.au/view/UQ:3

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kamli, Hossam. “Exploring resistance of renal cell carcinoma to sunitinib therapy.” 2020. Thesis, University of Queensland. Accessed January 17, 2021. http://espace.library.uq.edu.au/view/UQ:3.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kamli, Hossam. “Exploring resistance of renal cell carcinoma to sunitinib therapy.” 2020. Web. 17 Jan 2021.

Vancouver:

Kamli H. Exploring resistance of renal cell carcinoma to sunitinib therapy. [Internet] [Thesis]. University of Queensland; 2020. [cited 2021 Jan 17]. Available from: http://espace.library.uq.edu.au/view/UQ:3.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kamli H. Exploring resistance of renal cell carcinoma to sunitinib therapy. [Thesis]. University of Queensland; 2020. Available from: http://espace.library.uq.edu.au/view/UQ:3

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Victoria University

12. O'Connell, Brett. A study of rat skeletal muscle Troponin C isoforms.

Degree: phd, School of Biomedical Sciences, 2005, Victoria University

URL: http://vuir.vu.edu.au/15657/

► The investigations described in this thesis were prompted by an overall interest in the phenomenon of Troponin C (TnC) polymorphism in mammalian skeletal muscle. Gaining… (more)

Subjects/Keywords: 0601 Biochemistry and Cell Biology; 1101 Medical Biochemistry and Metabolomics; 0608 Zoology

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APA (6^th Edition):

O'Connell, B. (2005). A study of rat skeletal muscle Troponin C isoforms. (Doctoral Dissertation). Victoria University. Retrieved from http://vuir.vu.edu.au/15657/

Chicago Manual of Style (16^th Edition):

O'Connell, Brett. “A study of rat skeletal muscle Troponin C isoforms.” 2005. Doctoral Dissertation, Victoria University. Accessed January 17, 2021. http://vuir.vu.edu.au/15657/.

MLA Handbook (7^th Edition):

O'Connell, Brett. “A study of rat skeletal muscle Troponin C isoforms.” 2005. Web. 17 Jan 2021.

Vancouver:

O'Connell B. A study of rat skeletal muscle Troponin C isoforms. [Internet] [Doctoral dissertation]. Victoria University; 2005. [cited 2021 Jan 17]. Available from: http://vuir.vu.edu.au/15657/.

Council of Science Editors:

O'Connell B. A study of rat skeletal muscle Troponin C isoforms. [Doctoral Dissertation]. Victoria University; 2005. Available from: http://vuir.vu.edu.au/15657/

13. Gandhi, Disha M. Strategies for the Modulation of Protease Activated Receptors (PARs).

Degree: 2018, Marquette University

URL: https://epublications.marquette.edu/dissertations_mu/802

► Protease-activated receptors (PARs) are class A G protein-coupled receptors (GPCRs) with 4 subtypes (PAR 1 – 4) and with a unique mode of action. PARs… (more)

▼ Protease-activated receptors (PARs) are class A G protein-coupled receptors (GPCRs) with 4 subtypes (PAR 1 – 4) and with a unique mode of action. PARs are cleaved by extracellular proteases at the N-terminus, creating a new tethered ligand that activates the receptor and transduces biological signals into the cell. PARs have been implicated in various productive and pathological signals, including those related to thrombosis, inflammation, reperfusion injury, and cancer cell metastasis. Despite the fact that PARs are attractive as drug targets, their intramolecular mode of activation makes it challenging to modulate them with drugs in a selective manner, and only one PAR-targeting therapy, the PAR1 antagonist and antiplatelet drug vorapaxar, is currently approved by the FDA. Previously in the Dockendorff lab, compounds with a 1,3-diaminobenzene scaffold, called parmodulins, were identified as biased ligands of PAR1 that selectively inhibit Gαq-mediated signaling of PAR1 without significantly inhibiting Gα12/13-mediated signaling leading to platelet shape change. More recently, cytoprotective and anti-inflammatory effects of parmodulins in endothelial cells were identified that could be fruitful for the treatment of sepsis and myocardial infarction. We developed a Gαq-mediated intracellular Ca2+ mobilization (iCa2+) assay in endothelial cells to perform medium-throughput screening and characterization of parmodulins, and we demonstrated that they are reversible, negative allosteric modulators of PAR1, unlike ligands such as vorapaxar. We also extended structure-activity relationship (SAR) studies of parmodulins to identify analogues with potentially improved properties using medicinal chemistry, and these parmodulins are promising leads for safer antithrombotic and sepsis drugs. Additionally, we commenced investigations into the role of heteromeric PAR complexes in proliferative disorders such as restenosis and cancer cell metastasis. We synthesized a novel class of bivalent ligands targeting putative PAR1/2 heteromers and characterized them using our iCa2+ assay with endothelial cells (EA.hy926) and the breast cancer cell line MDA-MB-231. Such bivalent PAR ligands hold promise for the selective treatment of cancer and other proliferative disorders. Advisors/Committee Members: Dockendorff, Christopher, St. Maurice, Martin, Steinmetz, Mark.

Subjects/Keywords: Biochemistry; Cell Biology

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APA (6^th Edition):

Gandhi, D. M. (2018). Strategies for the Modulation of Protease Activated Receptors (PARs). (Thesis). Marquette University. Retrieved from https://epublications.marquette.edu/dissertations_mu/802

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gandhi, Disha M. “Strategies for the Modulation of Protease Activated Receptors (PARs).” 2018. Thesis, Marquette University. Accessed January 17, 2021. https://epublications.marquette.edu/dissertations_mu/802.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gandhi, Disha M. “Strategies for the Modulation of Protease Activated Receptors (PARs).” 2018. Web. 17 Jan 2021.

Vancouver:

Gandhi DM. Strategies for the Modulation of Protease Activated Receptors (PARs). [Internet] [Thesis]. Marquette University; 2018. [cited 2021 Jan 17]. Available from: https://epublications.marquette.edu/dissertations_mu/802.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gandhi DM. Strategies for the Modulation of Protease Activated Receptors (PARs). [Thesis]. Marquette University; 2018. Available from: https://epublications.marquette.edu/dissertations_mu/802

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Pennsylvania

14. Dawicki McKenna, Jennine. Investigating the Role of Microtubules in Glut4 Vesicle Trafficking and the Kinetics of Membrane Attachment by the Myosin Myo1c.

Degree: 2012, University of Pennsylvania

URL: https://repository.upenn.edu/edissertations/501

► The myosin myo1c dynamically localizes to cellular membranes through high affinity phosphoinositide binding and links them to the actin cytoskeleton. Determining the kinetics of membrane… (more)

▼ The myosin myo1c dynamically localizes to cellular membranes through high affinity phosphoinositide binding and links them to the actin cytoskeleton. Determining the kinetics of membrane attachment will provide insight into the relationship between membrane-attachment and actin-attachment lifetimes, and will also provide details about the regulation of membrane attachment. Stopped-flow spectroscopy was used to measure the binding and dissociation of a recombinant myo1c construct containing the tail and regulatory domains (myo1cIQ-tail) to and from 100 nm diameter large unilamellar vesicles (LUVs). The apparent second-order rate constant for association of myo1cIQ-tail with LUVs containing 2% phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) was approximately diffusion-limited. Myo1cIQ-tail dissociated from PtdIns(4,5)P2 at a slower rate (2.0 s-1) than the pleckstrin homology domain of phospholipase C-δ (PLCδ-PH) (13 s-1). The presence of additional anionic phospholipid reduced the myo1cIQ-tail dissociation rate constant > 50-fold, but marginally changed the dissociation rate of PLCδ-PH, suggesting that additional electrostatic interactions in myo1cIQ-tail help to stabilize binding. Remarkably, high concentrations of soluble inositol phosphates induce dissociation of myo1cIQ-tail from LUVs, suggesting that phosphoinositides are able to bind and dissociate from myo1cIQ-tail as it remains bound to the membrane. In adipocytes, vesicles containing glucose transporter-4 (GLUT4) redistribute from intracellular stores to the cell periphery in response to insulin. Vesicles then fuse with the plasma membrane, facilitating glucose transport into the cell. To gain insight into the molecular role of microtubules, we examined the spatial organization and dynamics of microtubules in relation to GLUT4 vesicle trafficking in living 3T3-L1 adipocytes using total internal reflection fluorescence (TIRF) microscopy. Insulin stimulated an increase in microtubule density and curvature within the TIRF-illuminated region of the cell. The time course of the density increase precedes that of the increase in intensity of HA-GLUT4-eGFP in this same region. Microtubule disruption delayed and modestly reduced the accumulation of GLUT4 at the plasma membrane. Interestingly, fusion of GLUT4-containing vesicles with the plasma membrane preferentially occur near microtubules, and long-distance vesicle movement along microtubules visible at the cell surface prior to fusion does not appear to account for this proximity. We conclude that microtubules may be important in providing spatial information for fusion events.

Subjects/Keywords: Biochemistry; Cell Biology

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APA (6^th Edition):

Dawicki McKenna, J. (2012). Investigating the Role of Microtubules in Glut4 Vesicle Trafficking and the Kinetics of Membrane Attachment by the Myosin Myo1c. (Thesis). University of Pennsylvania. Retrieved from https://repository.upenn.edu/edissertations/501

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dawicki McKenna, Jennine. “Investigating the Role of Microtubules in Glut4 Vesicle Trafficking and the Kinetics of Membrane Attachment by the Myosin Myo1c.” 2012. Thesis, University of Pennsylvania. Accessed January 17, 2021. https://repository.upenn.edu/edissertations/501.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dawicki McKenna, Jennine. “Investigating the Role of Microtubules in Glut4 Vesicle Trafficking and the Kinetics of Membrane Attachment by the Myosin Myo1c.” 2012. Web. 17 Jan 2021.

Vancouver:

Dawicki McKenna J. Investigating the Role of Microtubules in Glut4 Vesicle Trafficking and the Kinetics of Membrane Attachment by the Myosin Myo1c. [Internet] [Thesis]. University of Pennsylvania; 2012. [cited 2021 Jan 17]. Available from: https://repository.upenn.edu/edissertations/501.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dawicki McKenna J. Investigating the Role of Microtubules in Glut4 Vesicle Trafficking and the Kinetics of Membrane Attachment by the Myosin Myo1c. [Thesis]. University of Pennsylvania; 2012. Available from: https://repository.upenn.edu/edissertations/501

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Queensland

15. Doffek, Benjamin. From farm to pharm: taking plant pharmaceuticals from research to production.

Degree: Institute for Molecular Bioscience, 2020, University of Queensland

URL: https://espace.library.uq.edu.au/view/UQ:a238d17/s4443056_final_thesis.pdf ; https://espace.library.uq.edu.au/view/UQ:a238d17

► Affordable production of pharmaceuticals with high potency and low side effects is a major challenge of the 21st century. Peptides are an emerging class of… (more)

▼ Affordable production of pharmaceuticals with high potency and low side effects is a major challenge of the 21st century. Peptides are an emerging class of therapeutics that have the potential to marry the specificity and efficacy of protein drugs with the stability and membrane permeability of small molecule drugs. Although many peptides are amenable to chemical synthesis, their cost of production is high, as is the generation of waste products. Peptide production in plants has the potential to be a scalable, cost effective, and a less environmentally taxing alternative. Cyclotides, first discovered in Oldenlandia affinis, are a unique class of backbone cyclic peptides containing three stabilising disulfide bridges that form a knot-like structure. Their stability, and for some variants, the ability to traverse cellular membranes make them ideal candidates for pharmaceutical and agricultural applications. Even though highly constrained sterically, cyclotides are amenable to engineering by replacing native sequences with bioactive epitopes. In this thesis, cyclotide production strategies in plant cell suspensions are examined with a special focus placed on O. affinis as an archetypical cyclotide producer. Additional species investigated are Clitoria ternatea, Hybanthus enneaspermus, Nicotiana benthamiana, and Petunia hybrida. Insights into how cyclotides and their biosynthetic processing machinery are regulated in suspension plant cells are reported and provide first steps towards an affordable and environmentally friendly peptide production system in plants.Chapter 1 introduces the relevant scientific knowledge. The journey starts with a description of proteins and peptides, emphasising cyclotides and their synthesis. Then, current production strategies are compared with plant-based systems. The advantages and disadvantages of bioreactor setups suitable for medium to large scale production of cyclotides in plant cell suspensions are subsequently outlined. Finally, the importance of downstream processing is discussed. At the end of Chapter 1, the scope of this thesis is outlined to give an overview of the scientific questions addressed.Chapter 2 lays the experimental groundwork upon which the rest of this thesis is built. Protocols to establish C. ternatea, H. enneaspermus, N. benthamiana, O. affinis, and P. hybrida suspension cultures were developed and their cyclotide accumulation profiles presented. Cycloviolacin O2, a cyclotide previously reported only in the Violaceae, was discovered in O. affinis suspension cells and corroborated in the O. affinis leaf transcriptome. Two new cyclotides, kalata B22 and kalata B23, were characterized from O. affinis suspensions and were shown to have high sequence similarity to a group of cyclotides found in suspension cells of multiple plant families. Additionally, a strong time dependent effect was observed for cyclotide production in O. affinis suspension cells and was connected to reduced cyclotide mRNA expression. Cyclotide production was tissue specific in all tested…

Subjects/Keywords: Cyclotides; Plant cell suspension; Mass-cultivation; Wave-mixed bioreactor; Scale up; Molecular farming; Elicitation; Transformation; 0601 Biochemistry and Cell Biology; 1003 Industrial Biotechnology; 1004 Medical Biotechnology

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APA (6^th Edition):

Doffek, B. (2020). From farm to pharm: taking plant pharmaceuticals from research to production. (Thesis). University of Queensland. Retrieved from https://espace.library.uq.edu.au/view/UQ:a238d17/s4443056_final_thesis.pdf ; https://espace.library.uq.edu.au/view/UQ:a238d17

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Doffek, Benjamin. “From farm to pharm: taking plant pharmaceuticals from research to production.” 2020. Thesis, University of Queensland. Accessed January 17, 2021. https://espace.library.uq.edu.au/view/UQ:a238d17/s4443056_final_thesis.pdf ; https://espace.library.uq.edu.au/view/UQ:a238d17.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Doffek, Benjamin. “From farm to pharm: taking plant pharmaceuticals from research to production.” 2020. Web. 17 Jan 2021.

Vancouver:

Doffek B. From farm to pharm: taking plant pharmaceuticals from research to production. [Internet] [Thesis]. University of Queensland; 2020. [cited 2021 Jan 17]. Available from: https://espace.library.uq.edu.au/view/UQ:a238d17/s4443056_final_thesis.pdf ; https://espace.library.uq.edu.au/view/UQ:a238d17.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Doffek B. From farm to pharm: taking plant pharmaceuticals from research to production. [Thesis]. University of Queensland; 2020. Available from: https://espace.library.uq.edu.au/view/UQ:a238d17/s4443056_final_thesis.pdf ; https://espace.library.uq.edu.au/view/UQ:a238d17

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Wayne State University

16. Mcfarland, Timothy. Cardiac Calsequestrin Phosphorylation And Trafficking In The Mammalian Cardiomyocyte.

Degree: PhD, Physiology, 2011, Wayne State University

URL: https://digitalcommons.wayne.edu/oa_dissertations/176

► Cardiac CSQ (CSQ2) is a multifaceted protein, capable of binding significant quantities of Ca2+ and altering ryanodine receptor activity at the junctional sarcoplasmic reticulum… (more)

▼ Cardiac CSQ (CSQ2) is a multifaceted protein, capable of binding significant quantities of Ca2+ and altering ryanodine receptor activity at the junctional sarcoplasmic reticulum (SR). Little is known about the trafficking of CSQ2 from its unknown site of biosynthesis, which appears to be of importance as its structure changes in a trafficking-dependent manner in various types of heart failure. Through the use of multiple antibodies specific to classic rough ER markers, and with the creation of CSQ-DsRed tetramer fusion protein, we were able to establish a juxtanuclear localization of rough ER in cardiomyocytes. Using fluorescence confocal microscopy, the translocon complex proteins TRAP-α and TRAM, along with the ribosomal protein S6, were all visualized and found to encapsulate both myonuclei. Additionally, time course studies of CSQ-DsRed, in conjunction with anti-DsRed immunostaining, highlighted a perinuclear rough ER site of biosynthesis for CSQ2. The fusion protein exhibited a tetramerization-dependent trafficking predicted to be very similar to CSQ2 polymerization-dependent trafficking with high and low secretory compartment Ca2+ concentrations leading to polymerization and monomerization, respectively. Cardiac-specific C-terminal phosphorylation of CSQ2 serines was shown to effect CSQ2 trafficking according to mass spectrometric analysis of the protein's N-linked glycan. A Ser 378,382,386 Ala mutation (CSQ-nonPP) of these sites inhibited CSQ2 phosphorylation and led to an increased trafficking out of the ER as indicated by the increased mannose trimming of CSQ2 glycan. This effect was reversed with the phosphomimetic mutant Ser 378,382,386 Glu (CSQ-mimPP) which had glycoforms with mannose trimming nearly identical to that of CSQ-WT. Pharmacological and molecular inhibition were used to establish the identity of CSQ2 kinase as protein kinase CK2. In vitro, inhibition of CSQ2 phosphorylation using cardiomyocyte and nonmuscle cell CSQ2 kinase sources was successfully carried out with three specific inhibitors of CK2: TBB, DMAT and TBCA. TBCA produced identical inhibition curves for both cellular kinase sources and commercial CK2α′ kinase, with increasing concentrations of drug. SiRNA knockdown of both CK2α and CK2α′ catalytic subunits in nonmuscle cells reduced CSQ2 kinase activity by nearly 2-fold according to similar in vitro CSQ2 phosphorylation assays. Additionally, both ATP-binding site competitive inhibition and CK2 RNAi protein inhibition were used successfully in situ to suppress endogenous CSQ2 phosphorylation. TBCA used at 100 µM with CSQ-WT overexpressing cardiomyocytes and COS nonmuscle cells in culture led to an 82 and 66% decrease in CSQ2 phosphate incorporation, respectively, compared to control. Similarly, simultaneous knockdown of both CK2α and CK2α′ catalytic subunits in COS cells overexpressing adenoviral CSQ-WT, caused a near 2-fold decrease in CSQ2… Advisors/Committee Members: Steven Cala.

Subjects/Keywords: Biochemistry; Cell Biology; Molecular Biology

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APA (6^th Edition):

Mcfarland, T. (2011). Cardiac Calsequestrin Phosphorylation And Trafficking In The Mammalian Cardiomyocyte. (Doctoral Dissertation). Wayne State University. Retrieved from https://digitalcommons.wayne.edu/oa_dissertations/176

Chicago Manual of Style (16^th Edition):

Mcfarland, Timothy. “Cardiac Calsequestrin Phosphorylation And Trafficking In The Mammalian Cardiomyocyte.” 2011. Doctoral Dissertation, Wayne State University. Accessed January 17, 2021. https://digitalcommons.wayne.edu/oa_dissertations/176.

MLA Handbook (7^th Edition):

Mcfarland, Timothy. “Cardiac Calsequestrin Phosphorylation And Trafficking In The Mammalian Cardiomyocyte.” 2011. Web. 17 Jan 2021.

Vancouver:

Mcfarland T. Cardiac Calsequestrin Phosphorylation And Trafficking In The Mammalian Cardiomyocyte. [Internet] [Doctoral dissertation]. Wayne State University; 2011. [cited 2021 Jan 17]. Available from: https://digitalcommons.wayne.edu/oa_dissertations/176.

Council of Science Editors:

Mcfarland T. Cardiac Calsequestrin Phosphorylation And Trafficking In The Mammalian Cardiomyocyte. [Doctoral Dissertation]. Wayne State University; 2011. Available from: https://digitalcommons.wayne.edu/oa_dissertations/176

University of Minnesota

17. Rust, Brittney. Exosome-Mediated Protection Against Oxidative Stress: A Connection Between NRF2 And Exosomes.

Degree: MS, Chemistry, 2016, University of Minnesota

URL: http://hdl.handle.net/11299/202070

► The nuclear factor erythroid 2 (NF-E2)-related factor 2 (NRF2) transcription factor has been widely studied for its role as the master regulator of the response… (more)

Subjects/Keywords: Biochemistry; Biology; Cell Biology

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APA (6^th Edition):

Rust, B. (2016). Exosome-Mediated Protection Against Oxidative Stress: A Connection Between NRF2 And Exosomes. (Masters Thesis). University of Minnesota. Retrieved from http://hdl.handle.net/11299/202070

Chicago Manual of Style (16^th Edition):

Rust, Brittney. “Exosome-Mediated Protection Against Oxidative Stress: A Connection Between NRF2 And Exosomes.” 2016. Masters Thesis, University of Minnesota. Accessed January 17, 2021. http://hdl.handle.net/11299/202070.

MLA Handbook (7^th Edition):

Rust, Brittney. “Exosome-Mediated Protection Against Oxidative Stress: A Connection Between NRF2 And Exosomes.” 2016. Web. 17 Jan 2021.

Vancouver:

Rust B. Exosome-Mediated Protection Against Oxidative Stress: A Connection Between NRF2 And Exosomes. [Internet] [Masters thesis]. University of Minnesota; 2016. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/11299/202070.

Council of Science Editors:

Rust B. Exosome-Mediated Protection Against Oxidative Stress: A Connection Between NRF2 And Exosomes. [Masters Thesis]. University of Minnesota; 2016. Available from: http://hdl.handle.net/11299/202070

University of Arkansas

18. Alghanmi, Arwa Mohammed. The Structural and Functional Properties of a Double Mutant of Human Acidic Fibroblast Growth Factor (hFGF-1).

Degree: MS, 2017, University of Arkansas

URL: https://scholarworks.uark.edu/etd/2481

► Human acidic Fibroblast Growth Factor 1 (FGF-1), a member of the FGF superfamily, is a potent mitogen and heparin-binding protein involved in a broad… (more)

Subjects/Keywords: Biochemistry; Cell Biology; Molecular Biology

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APA (6^th Edition):

Alghanmi, A. M. (2017). The Structural and Functional Properties of a Double Mutant of Human Acidic Fibroblast Growth Factor (hFGF-1). (Masters Thesis). University of Arkansas. Retrieved from https://scholarworks.uark.edu/etd/2481

Chicago Manual of Style (16^th Edition):

Alghanmi, Arwa Mohammed. “The Structural and Functional Properties of a Double Mutant of Human Acidic Fibroblast Growth Factor (hFGF-1).” 2017. Masters Thesis, University of Arkansas. Accessed January 17, 2021. https://scholarworks.uark.edu/etd/2481.

MLA Handbook (7^th Edition):

Alghanmi, Arwa Mohammed. “The Structural and Functional Properties of a Double Mutant of Human Acidic Fibroblast Growth Factor (hFGF-1).” 2017. Web. 17 Jan 2021.

Vancouver:

Alghanmi AM. The Structural and Functional Properties of a Double Mutant of Human Acidic Fibroblast Growth Factor (hFGF-1). [Internet] [Masters thesis]. University of Arkansas; 2017. [cited 2021 Jan 17]. Available from: https://scholarworks.uark.edu/etd/2481.

Council of Science Editors:

Alghanmi AM. The Structural and Functional Properties of a Double Mutant of Human Acidic Fibroblast Growth Factor (hFGF-1). [Masters Thesis]. University of Arkansas; 2017. Available from: https://scholarworks.uark.edu/etd/2481

University of Arkansas

19. ALJahdali, Nesreen Hamdan. Determination of the Effects of Different Maillard Reaction Products on the Taxonomic Composition of the Gut Microbiota.

Degree: PhD, 2017, University of Arkansas

URL: https://scholarworks.uark.edu/etd/2569

► The Maillard Reaction (MR) is a non-enzymatic chemical reaction which results in linkage between the amino group of amino acids and the carbonyl group… (more)

▼ The Maillard Reaction (MR) is a non-enzymatic chemical reaction which results in linkage between the amino group of amino acids and the carbonyl group of reduced sugars. This reaction generates Maillard reaction products (MRPs) which are not present naturally in foods, and are responsible for a range of colors, odors, flavors, and other sensory properties. Conflicting reports of MRPs impacts on human health are probably due to the fact that bioconversion of these digestible molecules by the gut microbiota has been marginally taken into account. This study aimed to determine the effects of different MRPs on rodent’s gut microbiota through16S rRNA amplicon sequencing over three different studies. Study 1 focused on the impact of NƐCarboxymethyllysine (CML) on the composition of mice gut microbiota and potential association with severity of experimental colitis. Study 2 focused on the impact of bread melanoidins on the composition of healthy and experimental colitis mice gut microbiota. Study 3 focused on the impact of consumption of increasing amounts of malt melanoidins on mice gut microbiota. It was found that CML induced limited changes in gut microbiota profiles of healthy mice, but was found to significantly relieve the bacterial dysbiosis imparted by one (but not the other) inflammation-inducing chemical, especially the Proteobacteria bloom. Bread crust model (high in melanoidins) showed significant decreases of Bacteroides spp. and Enterobacteriaceae, while it increased Faecalibacterium spp. Also, bread crust model limited to increase Enterobacteriaceae in colitis model. High amounts of malts rich melanoidins rapidly and persistently led to significantly different gut microbiota profiles. There was a trend for decrease of Lactobacillus and Ruminococcus and increase of Akkermansia and Bifidobacterium with higher amounts of dietary melanoidins. We concluded that CML and melanoidins are not detrimental in terms of their impact on the gut microbiota, and that they may even have prebiotic properties. Advisors/Committee Members: Franck Carbonero, Steve Ricke, Sami Dridi.

Subjects/Keywords: Biochemistry; Cell Biology; Molecular Biology

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APA (6^th Edition):

ALJahdali, N. H. (2017). Determination of the Effects of Different Maillard Reaction Products on the Taxonomic Composition of the Gut Microbiota. (Doctoral Dissertation). University of Arkansas. Retrieved from https://scholarworks.uark.edu/etd/2569

Chicago Manual of Style (16^th Edition):

ALJahdali, Nesreen Hamdan. “Determination of the Effects of Different Maillard Reaction Products on the Taxonomic Composition of the Gut Microbiota.” 2017. Doctoral Dissertation, University of Arkansas. Accessed January 17, 2021. https://scholarworks.uark.edu/etd/2569.

MLA Handbook (7^th Edition):

ALJahdali, Nesreen Hamdan. “Determination of the Effects of Different Maillard Reaction Products on the Taxonomic Composition of the Gut Microbiota.” 2017. Web. 17 Jan 2021.

Vancouver:

ALJahdali NH. Determination of the Effects of Different Maillard Reaction Products on the Taxonomic Composition of the Gut Microbiota. [Internet] [Doctoral dissertation]. University of Arkansas; 2017. [cited 2021 Jan 17]. Available from: https://scholarworks.uark.edu/etd/2569.

Council of Science Editors:

ALJahdali NH. Determination of the Effects of Different Maillard Reaction Products on the Taxonomic Composition of the Gut Microbiota. [Doctoral Dissertation]. University of Arkansas; 2017. Available from: https://scholarworks.uark.edu/etd/2569

20. St. Germain, Bryan J. Connecting Motors and Membranes: A Quantitative Investigation of Dynein Pathway Components and in vitro Characterization of the Num1 Coiled Coil Domain.

Degree: Master of Architecture (M.Arch.), Molecular & Cellular Biology, 2011, University of Massachusetts

URL: https://scholarworks.umass.edu/theses/720

► In the budding yeast, Saccharomyces Cerevisiae, dynein, a minus-end directed motor, is involved in nuclear migration and proper orientation of the mitotic spindle during… (more)

▼ In the budding yeast, Saccharomyces Cerevisiae, dynein, a minus-end directed motor, is involved in nuclear migration and proper orientation of the mitotic spindle during mitosis. Our lab has developed a model that involves the loading of cytoplasmic dynein onto the plus-end of astral microtubules through interactions with Pac1/LIS1 and Bik1/CLIP-170. Dynein is then delivered to the cell cortex and anchored through a cortical receptor protein, Num1. Num1 is a 313KDa protein that localizes to the cell cortex and is an essential component of dynein mediated nuclear migration. Using quantitative fluorescence techniques I was able to create a molecular inventory of various dynein pathway components. Our results revealed Dyn1, dynein heavy chain, and Pac1/LIS1 associate at the plus end in a 1:1 ratio. Additionally we found that dynein and dynactin associate in a 3:1 ratio at the plus ends and a 2:1 ratio at the cortex. Interestingly, we found that over expression of Pac1/LIS1 augments cortical dynein activity while maintaining the dynein to dynactin ratio and this activity is separate from loss of She1, a negative regulator of dynein-dynactin interaction, which results in a 1:1 ratio of dynein-dynactin at the plus-ends, as well as, the cortex. Our results uncover molecular ratios that enable us to create more defined and detailed model of the dynein pathway. To elucidate how Num1 attaches dynein to the cortex we created truncations of the Num1 protein. We were able to determine that two coiled-coil (CC) domains in the N-terminus of Num1 are responsible for bright foci formation on the cortex. Cells without these bright foci exhibit a binucleate phenotype similar to that of dyn1∆ implicating that these bright foci are required for the proper function of Num1 in the dynein pathway. To test the hypothesis that the CC is capable of mediated bright patch assembly through self-association I purified a recombinant CC domain and performed gel filtration analysis, as well as, equilibrium sedimentation. I was able to determine that the CC domain exists as a dimer in solution. However, the mechanism of CC self-assembly may involve a requisite of targeting the CC to the cortex first. Advisors/Committee Members: Wei-Lih Lee.

Subjects/Keywords: Biochemistry; Cell Biology; Molecular Biology

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APA (6^th Edition):

St. Germain, B. J. (2011). Connecting Motors and Membranes: A Quantitative Investigation of Dynein Pathway Components and in vitro Characterization of the Num1 Coiled Coil Domain. (Masters Thesis). University of Massachusetts. Retrieved from https://scholarworks.umass.edu/theses/720

Chicago Manual of Style (16^th Edition):

St. Germain, Bryan J. “Connecting Motors and Membranes: A Quantitative Investigation of Dynein Pathway Components and in vitro Characterization of the Num1 Coiled Coil Domain.” 2011. Masters Thesis, University of Massachusetts. Accessed January 17, 2021. https://scholarworks.umass.edu/theses/720.

MLA Handbook (7^th Edition):

St. Germain, Bryan J. “Connecting Motors and Membranes: A Quantitative Investigation of Dynein Pathway Components and in vitro Characterization of the Num1 Coiled Coil Domain.” 2011. Web. 17 Jan 2021.

Vancouver:

St. Germain BJ. Connecting Motors and Membranes: A Quantitative Investigation of Dynein Pathway Components and in vitro Characterization of the Num1 Coiled Coil Domain. [Internet] [Masters thesis]. University of Massachusetts; 2011. [cited 2021 Jan 17]. Available from: https://scholarworks.umass.edu/theses/720.

Council of Science Editors:

St. Germain BJ. Connecting Motors and Membranes: A Quantitative Investigation of Dynein Pathway Components and in vitro Characterization of the Num1 Coiled Coil Domain. [Masters Thesis]. University of Massachusetts; 2011. Available from: https://scholarworks.umass.edu/theses/720

Victoria University of Technology

21. Yuriev, Elizabeth. Steric aspects of the interaction of metal species with nucleic acid constituents.

Degree: phd, Department of Chemical Sciences, 1997, Victoria University of Technology

URL: http://vuir.vu.edu.au/15393/

► The delineation of steric effects in the interaction of metal species with biomolecules has been addressed. More specifically, a focus has been placed on the… (more)

Subjects/Keywords: 0305 Organic Chemistry; 0601 Biochemistry and Cell Biology; School of Engineering and Science

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APA (6^th Edition):

Yuriev, E. (1997). Steric aspects of the interaction of metal species with nucleic acid constituents. (Doctoral Dissertation). Victoria University of Technology. Retrieved from http://vuir.vu.edu.au/15393/

Chicago Manual of Style (16^th Edition):

Yuriev, Elizabeth. “Steric aspects of the interaction of metal species with nucleic acid constituents.” 1997. Doctoral Dissertation, Victoria University of Technology. Accessed January 17, 2021. http://vuir.vu.edu.au/15393/.

MLA Handbook (7^th Edition):

Yuriev, Elizabeth. “Steric aspects of the interaction of metal species with nucleic acid constituents.” 1997. Web. 17 Jan 2021.

Vancouver:

Yuriev E. Steric aspects of the interaction of metal species with nucleic acid constituents. [Internet] [Doctoral dissertation]. Victoria University of Technology; 1997. [cited 2021 Jan 17]. Available from: http://vuir.vu.edu.au/15393/.

Council of Science Editors:

Yuriev E. Steric aspects of the interaction of metal species with nucleic acid constituents. [Doctoral Dissertation]. Victoria University of Technology; 1997. Available from: http://vuir.vu.edu.au/15393/

22. Soo, Rochelle Melissa. In search of non-photosynthetic Cyanobacteria.

Degree: School of Chemistry and Molecular Biosciences, 2015, University of Queensland

URL: http://espace.library.uq.edu.au/view/UQ:368958

Subjects/Keywords: Melainabacteria; Cyanobacteria; Metabolism; 0601 Biochemistry and Cell Biology; 0605 Microbiology

…Microbiology, 70% FoR code: 0601, Biochemistry and cell biology, 30% viii Table of Contents… …Cell Metabolism, 30% Fields of Research (FoR) Classification FoR code: 0605… …68 3.4.3 Cell shape and envelope… …91 4.4.4 Cell wall and shape… …103 4.4.10.7 Cell membrane adaptations…

10 more images…

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APA (6^th Edition):

Soo, R. M. (2015). In search of non-photosynthetic Cyanobacteria. (Thesis). University of Queensland. Retrieved from http://espace.library.uq.edu.au/view/UQ:368958

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Soo, Rochelle Melissa. “In search of non-photosynthetic Cyanobacteria.” 2015. Thesis, University of Queensland. Accessed January 17, 2021. http://espace.library.uq.edu.au/view/UQ:368958.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Soo, Rochelle Melissa. “In search of non-photosynthetic Cyanobacteria.” 2015. Web. 17 Jan 2021.

Vancouver:

Soo RM. In search of non-photosynthetic Cyanobacteria. [Internet] [Thesis]. University of Queensland; 2015. [cited 2021 Jan 17]. Available from: http://espace.library.uq.edu.au/view/UQ:368958.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Soo RM. In search of non-photosynthetic Cyanobacteria. [Thesis]. University of Queensland; 2015. Available from: http://espace.library.uq.edu.au/view/UQ:368958

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Queensland

23. Tong, Samuel Jia Ming. Regulation of Rab8 in Toll-Like Receptor Signalling Pathways.

Degree: Institute for Molecular Bioscience, 2020, University of Queensland

URL: http://espace.library.uq.edu.au/view/UQ:9

► Small GTPases of the Rab family have wide ranging and essential roles in recruiting effectors to mediate membrane trafficking and receptor signalling events in mammalian… (more)

▼ Small GTPases of the Rab family have wide ranging and essential roles in recruiting effectors to mediate membrane trafficking and receptor signalling events in mammalian cells. Nucleotide loading and activation/deactivation of the Rabs themselves are regulated by guanine nucleotide exchange factors (GEFs), GTPase activating proteins (GAPs) and other accessory proteins. While Rabs have important roles in innate immune cells, for host-pathogen interactions, receptor signalling and membrane trafficking, in many cases their GEFs and other accessory proteins have not been elucidated.Pathogen-activated, Toll-like receptors (TLRs) initiate and modulate transcription of inflammatory cytokines and other innate immune responses which are important for immune defence but can also contribute to chronic disease. Macrophages are key cells of the innate immune system and previous work from our laboratory established a role for promiscuous Rab family member, Rab8a, in TLR signalling. Rab8a is activated in TLR pathways to recruit phosphoinositide 3-kinase gamma (PI3Kg) as an effector which upregulates TLR-induced Akt/mTOR signalling to drive a biased program of anti-inflammatory cytokines. TLR-associated PI3Kg has now emerged as a key mediator of macrophage programming (or polarisation) in inflammation and cancer and its cognate GTPase, Rab8a, has a prominent role in immunity and disease. Rab8a is itself recruited to macrophage ruffles and macropinosomes through its association with the TLR crosstalk-activated endocytic receptor LRP1. However, it is not yet known how Rab8a is activated in TLR pathways. This project set out to identify the essential Rab8a GEF(s) needed to activate Rab8a as part of the LRP1 complex.Two well-known Rab8 GEFs, GRAB and Rabin8, which previously were uncharacterised in macrophages, were investigated in this project. GRAB was identified as part of the low density lipoprotein receptor-related protein 1 (LRP1) complex in pull-downs analysed by mass spectrometry. Co-immunoprecipitation and fluorescence microscopy showed that both GRAB and the structurally similar GEF, Rabin8, undergo LPS-inducible binding to Rab8a and are localised at sites of Rab8a enrichment. To carry out functional studies, stable knockouts (KOs) of Rabin8, GRAB, as well as a double KO were produced via CRISPR-Cas9 gene editing in macrophage cell lines. Nucleotide activation assays were developed for this project and live-cell imaging with KO cell lines showed that both GEFs contribute additively to TLR4-induced Rab8a GTP-loading, but they are not needed for Rab8a membrane recruitment. Analysis of TLR signalling after double KO of both GEFs suggested redundant roles for Rabin8 and GRAB in activating Rab8a for PI3Kg-dependent Akt/mTOR signalling. Live cell imaging utilising a fluorescent Akt1 reporter confirmed that LPS/TLR-induced Akt signalling is generated on macropinosomes and requires GRAB and Rabin8 GEF function.Next, to investigate possible regulators of the Rab8 GEFs, pull-downs and mass spectrometry were performed and identified a…

Subjects/Keywords: Macrophages; Rab GTPases; Rab8; Guanine nucleotide exchange factors; GRAB; Rabin8; Toll-Like receptors; PI3Kgamma; LRP1; Inflammation; 0601 Biochemistry and Cell Biology; 1107 Immunology

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APA (6^th Edition):

Tong, S. J. M. (2020). Regulation of Rab8 in Toll-Like Receptor Signalling Pathways. (Thesis). University of Queensland. Retrieved from http://espace.library.uq.edu.au/view/UQ:9

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tong, Samuel Jia Ming. “Regulation of Rab8 in Toll-Like Receptor Signalling Pathways.” 2020. Thesis, University of Queensland. Accessed January 17, 2021. http://espace.library.uq.edu.au/view/UQ:9.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tong, Samuel Jia Ming. “Regulation of Rab8 in Toll-Like Receptor Signalling Pathways.” 2020. Web. 17 Jan 2021.

Vancouver:

Tong SJM. Regulation of Rab8 in Toll-Like Receptor Signalling Pathways. [Internet] [Thesis]. University of Queensland; 2020. [cited 2021 Jan 17]. Available from: http://espace.library.uq.edu.au/view/UQ:9.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tong SJM. Regulation of Rab8 in Toll-Like Receptor Signalling Pathways. [Thesis]. University of Queensland; 2020. Available from: http://espace.library.uq.edu.au/view/UQ:9

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Queensland

24. Forouz, Farzaneh. Enhanced topical drug delivery for treatment of human melanoma.

Degree: Faculty of Medicine, 2020, University of Queensland

URL: http://espace.library.uq.edu.au/view/UQ:0

► The main objective of this thesis is to enhance topical delivery targeting early stage cutaneous melanoma aiming to increase therapeutic effects using optimal formulations. To… (more)

▼ The main objective of this thesis is to enhance topical delivery targeting early stage cutaneous melanoma aiming to increase therapeutic effects using optimal formulations. To achieve this primary aims, various approaches were applied such as (i) formulation behaviour studies using in vitro kinetic analysis, (ii) pre-formulation toxicity studies to select the safe raw ingredients, and (iii) Self-Emulsifying Microemulsion (SEME) systems was to enhance the drug delivery while reducing the toxicity.Early research was initiated by screening some small molecules with potential to be used as anti-melanoma agents, incorporated in topical formulations. In vitro cell culture of human melanoma and non-melanoma skin cells was employed as the biological model. Rose Bengal and coumarin were selected as active agents and classified as compounds with high and low potency based on the IC50 ranking. Different formulation approaches were considered for each compound according to their potency and physicochemical properties to enhance their bioavailability, anti-proliferative activity and minimize skin irritancy.Chapter 3 focuses on the relationship between the physicochemical properties of coumarin and its skin permeation from simple ternary solutions. Understanding of thermodynamic activity/solubility parameters of coumarin in delivery systems is used as an approach to enhance solubility and penetration of the drug and thus improving the anti-melanoma effect at the target site. The aim is to explore the impact of various solutions containing three universal solvents on skin permeation of drug and the performance of the final product through assessing the relationships between drug-vehicle, drug-skin, or drug-vehicle-skin interaction. Comparison of in vitro penetration fluxes (Jmax30%) and solubility of the drug in vehicles (Sv) was undertaken. Formulation 5 containing 70% Eth: 10% PG: 20% water showed the highest delivery for coumarin across the skin with the maximum flux of 1281.4 (µg/cm2/h).The relationship between solubility of coumarin in vehicles with Jmax showed that coumarin penetration through the skin is mainly depends on the solubility in the vehicles. Mechanism of Eth and PG to enhance permeation was through increasing the solubility of coumarin in the vehicle and in the stratum corneum, leading to improved partitioning. Enhancements in stratum corneum solubility were found to be related to uptake of solvents. These results showed that it is possible to enhance targeted efficacy of coumarin in skin by enhancing the solubility of the drug in formulation. HSP approach showed to be valuable in predicting the parameters that influence permeation of coumarin in various vehicles.In Chapter 4, in vitro toxicology of all formulation ingredients was evaluated on various skin cell lines to understand the toxic effects of the excipients for clinical applications. The toxicity level was measured by means of cell viability (IC50) using two distinct endpoint assays: MTT and Crystal violet. The MTT assay measures the reduction of MTT…

Subjects/Keywords: Melanoma; Topical delivery; Cytotoxicity; MTT; Flowcytometry; Fluorescence lifetime imaging; Thermodynamic; Rose Bengal; coumarin; Skin permeation; 0601 Biochemistry and Cell Biology; 1115 Pharmacology and Pharmaceutical Sciences

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APA (6^th Edition):

Forouz, F. (2020). Enhanced topical drug delivery for treatment of human melanoma. (Thesis). University of Queensland. Retrieved from http://espace.library.uq.edu.au/view/UQ:0

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Forouz, Farzaneh. “Enhanced topical drug delivery for treatment of human melanoma.” 2020. Thesis, University of Queensland. Accessed January 17, 2021. http://espace.library.uq.edu.au/view/UQ:0.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Forouz, Farzaneh. “Enhanced topical drug delivery for treatment of human melanoma.” 2020. Web. 17 Jan 2021.

Vancouver:

Forouz F. Enhanced topical drug delivery for treatment of human melanoma. [Internet] [Thesis]. University of Queensland; 2020. [cited 2021 Jan 17]. Available from: http://espace.library.uq.edu.au/view/UQ:0.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Forouz F. Enhanced topical drug delivery for treatment of human melanoma. [Thesis]. University of Queensland; 2020. Available from: http://espace.library.uq.edu.au/view/UQ:0

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Queensland

25. Addala, Venkateswara. Therapeutic Opportunity in Cancers with Poor Survival.

Degree: Faculty of Medicine, 2020, University of Queensland

URL: http://espace.library.uq.edu.au/view/UQ:9

► Next generation sequencing has allowed researchers to characterise the somatic landscape of cancer genomes, which has led to the discovery of biomarkers that may be… (more)

▼ Next generation sequencing has allowed researchers to characterise the somatic landscape of cancer genomes, which has led to the discovery of biomarkers that may be predictive and prognostic to targeted therapies. However, the efficacy of current targeted therapies has failed to raise the overall survival curve in many tumour types. Immunotherapy has shown a promising benefit in treating many tumours and demonstrated remarkable responses even at recurrent, relapse and metastasis stage. Tumour mutation burden (TMB) can stratify some responders and non-responders to Immunotherapies, but it is not perfect. Tumour whole genome sequencing (WGS) and RNA sequencing play a major role in deciphering tumour intrinsic-extrinsic factors and may improve prediction responses to immune checkpoint blockade therapies. This dissertation will explore computational tools that can be applied to understand complex tumour-immune milieu and that may predict a response to immunotherapy.Chapter 2 includes a comprehensive benchmarking analysis of bioinformatics tools to identify cancer-immunity interactions. In addition, tools to predict responses for immune checkpoint blockade therapies were evaluated on publicly available immunotherapy treated clinical datasets. Using this information an immunogenomics pipeline was developed with the bioinformatic tools that showed the best concordance with gold-standard in-vitro techniques. This pipeline was applied in subsequent chapters to predict immunotherapy responses and to identify various tumour immune escape mechanisms in tumour types that have a poor survival.In Chapter 3, I used the immunogenomics pipeline to analyse whole genome sequencing and RNA sequencing of malignant pleural mesothelioma (MPM). MPM is a rare, aggressive cancer associated with asbestos exposure and has very limited treatment options. Heterogeneity was observed in tumour intrinsic and extrinsic factors within MPM patient samples, including clonal selection of neoantigen presentation, altered gene expression profiles in neoantigen trafficking components and enriched immune cells within the tumour microenvironment. This profiling may help in to increase overall survival in mesothelioma by indicating patients who are more likely to be responsive to emerging immunotherapies and importantly providing mechanisms for why other patients may not respond.In chapter 4, I assessed various immune escape mechanisms of microsatellite stable (MSS) primary colorectal and matched liver metastasis tumours. MSS stable tumours are generally thought to be immunogenic “cold” tumours and remain as a major research challenge as they exist in an immune suppressed tumour microenvironment with low TMB. Through matched primary and metastasis WGS and RNAseq analysis I identified loss of heterozygosity of the HLA alleles, consistent neoantigen profiles, heterogeneity in T-cell repertoire expansion and diversity and other tumour microenvironment components that facilitate immune escape mechanisms in “cold” tumours. These findings highlight the mechanisms…

Subjects/Keywords: cancer immunotherapy; immune checkpoint blockade; neoantigens; whole genome sequencing; rna sequencing; mesothelioma; mss cold tumours; melanoma; tumour microenvironment; immune cells deconvolution; 0601 Biochemistry and Cell Biology; 0604 Genetics; 1107 Immunology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Addala, V. (2020). Therapeutic Opportunity in Cancers with Poor Survival. (Thesis). University of Queensland. Retrieved from http://espace.library.uq.edu.au/view/UQ:9

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Addala, Venkateswara. “Therapeutic Opportunity in Cancers with Poor Survival.” 2020. Thesis, University of Queensland. Accessed January 17, 2021. http://espace.library.uq.edu.au/view/UQ:9.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Addala, Venkateswara. “Therapeutic Opportunity in Cancers with Poor Survival.” 2020. Web. 17 Jan 2021.

Vancouver:

Addala V. Therapeutic Opportunity in Cancers with Poor Survival. [Internet] [Thesis]. University of Queensland; 2020. [cited 2021 Jan 17]. Available from: http://espace.library.uq.edu.au/view/UQ:9.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Addala V. Therapeutic Opportunity in Cancers with Poor Survival. [Thesis]. University of Queensland; 2020. Available from: http://espace.library.uq.edu.au/view/UQ:9

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Victoria University

26. Kutyna, Dariusz Roman. Isolation of low ethanol producing yeast strains using adaptive evolution.

Degree: phd, School of Molecular Sciences, 2008, Victoria University

URL: http://vuir.vu.edu.au/15484/

► This thesis describes the application of a non-genetic engineering approach, adaptive evolution, to generate variants of Saccharomyces cerevisiae that produce reduced levels of ethanol relative… (more)

Subjects/Keywords: 0908 Food Sciences; 0601 Biochemistry and Cell Biology; School of Engineering and Science

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kutyna, D. R. (2008). Isolation of low ethanol producing yeast strains using adaptive evolution. (Doctoral Dissertation). Victoria University. Retrieved from http://vuir.vu.edu.au/15484/

Chicago Manual of Style (16^th Edition):

Kutyna, Dariusz Roman. “Isolation of low ethanol producing yeast strains using adaptive evolution.” 2008. Doctoral Dissertation, Victoria University. Accessed January 17, 2021. http://vuir.vu.edu.au/15484/.

MLA Handbook (7^th Edition):

Kutyna, Dariusz Roman. “Isolation of low ethanol producing yeast strains using adaptive evolution.” 2008. Web. 17 Jan 2021.

Vancouver:

Kutyna DR. Isolation of low ethanol producing yeast strains using adaptive evolution. [Internet] [Doctoral dissertation]. Victoria University; 2008. [cited 2021 Jan 17]. Available from: http://vuir.vu.edu.au/15484/.

Council of Science Editors:

Kutyna DR. Isolation of low ethanol producing yeast strains using adaptive evolution. [Doctoral Dissertation]. Victoria University; 2008. Available from: http://vuir.vu.edu.au/15484/

Victoria University

27. Vaughn, Michelle. Blonde hair colour: classification, characterisation and genetic associations for use in forensic science.

Degree: phd, School of Biomedical and Health Sciences, 2010, Victoria University

URL: http://vuir.vu.edu.au/15540/

► The vast variety of normal pigmentation variation is a huge genetic puzzle and hair colour is one part of this puzzle. It is hypothesised that… (more)

Subjects/Keywords: 0601 Biochemistry and Cell Biology; 0604 Genetics; School of Biomedical and Health Sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vaughn, M. (2010). Blonde hair colour: classification, characterisation and genetic associations for use in forensic science. (Doctoral Dissertation). Victoria University. Retrieved from http://vuir.vu.edu.au/15540/

Chicago Manual of Style (16^th Edition):

Vaughn, Michelle. “Blonde hair colour: classification, characterisation and genetic associations for use in forensic science.” 2010. Doctoral Dissertation, Victoria University. Accessed January 17, 2021. http://vuir.vu.edu.au/15540/.

MLA Handbook (7^th Edition):

Vaughn, Michelle. “Blonde hair colour: classification, characterisation and genetic associations for use in forensic science.” 2010. Web. 17 Jan 2021.

Vancouver:

Vaughn M. Blonde hair colour: classification, characterisation and genetic associations for use in forensic science. [Internet] [Doctoral dissertation]. Victoria University; 2010. [cited 2021 Jan 17]. Available from: http://vuir.vu.edu.au/15540/.

Council of Science Editors:

Vaughn M. Blonde hair colour: classification, characterisation and genetic associations for use in forensic science. [Doctoral Dissertation]. Victoria University; 2010. Available from: http://vuir.vu.edu.au/15540/

Victoria University of Technology

28. Nguyen, Long Thanh. A study of the role of glycogen in skeletal muscle performance and of myosin heavy chain isoform expression in amphibian skeletal muscle using the toad Bufo marinus.

Degree: phd, School of Life Sciences and Technology, 2000, Victoria University of Technology

URL: http://vuir.vu.edu.au/15310/

► The overall aim of this study was to contribute knowledge to two areas of inquiry in muscle research: one concerned with the molecular mechanism(s) underlying… (more)

Subjects/Keywords: 0606 Physiology; 0601 Biochemistry and Cell Biology; School of Engineering and Science

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nguyen, L. T. (2000). A study of the role of glycogen in skeletal muscle performance and of myosin heavy chain isoform expression in amphibian skeletal muscle using the toad Bufo marinus. (Doctoral Dissertation). Victoria University of Technology. Retrieved from http://vuir.vu.edu.au/15310/

Chicago Manual of Style (16^th Edition):

Nguyen, Long Thanh. “A study of the role of glycogen in skeletal muscle performance and of myosin heavy chain isoform expression in amphibian skeletal muscle using the toad Bufo marinus.” 2000. Doctoral Dissertation, Victoria University of Technology. Accessed January 17, 2021. http://vuir.vu.edu.au/15310/.

MLA Handbook (7^th Edition):

Nguyen, Long Thanh. “A study of the role of glycogen in skeletal muscle performance and of myosin heavy chain isoform expression in amphibian skeletal muscle using the toad Bufo marinus.” 2000. Web. 17 Jan 2021.

Vancouver:

Nguyen LT. A study of the role of glycogen in skeletal muscle performance and of myosin heavy chain isoform expression in amphibian skeletal muscle using the toad Bufo marinus. [Internet] [Doctoral dissertation]. Victoria University of Technology; 2000. [cited 2021 Jan 17]. Available from: http://vuir.vu.edu.au/15310/.

Council of Science Editors:

Nguyen LT. A study of the role of glycogen in skeletal muscle performance and of myosin heavy chain isoform expression in amphibian skeletal muscle using the toad Bufo marinus. [Doctoral Dissertation]. Victoria University of Technology; 2000. Available from: http://vuir.vu.edu.au/15310/

Victoria University

29. Hewakapuge, Sudinna Kulangana. Prediction of age from DNA.

Degree: phd, School of Biomedical Sciences, 2009, Victoria University

URL: http://vuir.vu.edu.au/15226/

► Currently DNA profiling methods only compare a suspect’s DNA with DNA left at the crime scene. When there is no suspect, it would be useful… (more)

Subjects/Keywords: 0601 Biochemistry and Cell Biology; 0604 Genetics; School of Biomedical and Health Sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hewakapuge, S. K. (2009). Prediction of age from DNA. (Doctoral Dissertation). Victoria University. Retrieved from http://vuir.vu.edu.au/15226/

Chicago Manual of Style (16^th Edition):

Hewakapuge, Sudinna Kulangana. “Prediction of age from DNA.” 2009. Doctoral Dissertation, Victoria University. Accessed January 17, 2021. http://vuir.vu.edu.au/15226/.

MLA Handbook (7^th Edition):

Hewakapuge, Sudinna Kulangana. “Prediction of age from DNA.” 2009. Web. 17 Jan 2021.

Vancouver:

Hewakapuge SK. Prediction of age from DNA. [Internet] [Doctoral dissertation]. Victoria University; 2009. [cited 2021 Jan 17]. Available from: http://vuir.vu.edu.au/15226/.

Council of Science Editors:

Hewakapuge SK. Prediction of age from DNA. [Doctoral Dissertation]. Victoria University; 2009. Available from: http://vuir.vu.edu.au/15226/

Victoria University of Technology

30. Jang, Ki-Hyo. Identification and characterisation of the cell surface and enzymatic barriers to plasmid transformation in Corynebacterium glutamicum and related species.

Degree: phd, Department of Biological and Food Technology, 1998, Victoria University of Technology

URL: http://vuir.vu.edu.au/15345/

► Non-pathogenic corynebacteria including Corynebacterium glutamicum, Brevibacterium flavum, and B. lactofermentum have been used traditionally for industrial production of amino acids. Recently, these strains have been… (more)

Subjects/Keywords: 0601 Biochemistry and Cell Biology; School of Engineering and Science

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jang, K. (1998). Identification and characterisation of the cell surface and enzymatic barriers to plasmid transformation in Corynebacterium glutamicum and related species. (Doctoral Dissertation). Victoria University of Technology. Retrieved from http://vuir.vu.edu.au/15345/

Chicago Manual of Style (16^th Edition):

Jang, Ki-Hyo. “Identification and characterisation of the cell surface and enzymatic barriers to plasmid transformation in Corynebacterium glutamicum and related species.” 1998. Doctoral Dissertation, Victoria University of Technology. Accessed January 17, 2021. http://vuir.vu.edu.au/15345/.

MLA Handbook (7^th Edition):

Jang, Ki-Hyo. “Identification and characterisation of the cell surface and enzymatic barriers to plasmid transformation in Corynebacterium glutamicum and related species.” 1998. Web. 17 Jan 2021.

Vancouver:

Jang K. Identification and characterisation of the cell surface and enzymatic barriers to plasmid transformation in Corynebacterium glutamicum and related species. [Internet] [Doctoral dissertation]. Victoria University of Technology; 1998. [cited 2021 Jan 17]. Available from: http://vuir.vu.edu.au/15345/.

Council of Science Editors:

Jang K. Identification and characterisation of the cell surface and enzymatic barriers to plasmid transformation in Corynebacterium glutamicum and related species. [Doctoral Dissertation]. Victoria University of Technology; 1998. Available from: http://vuir.vu.edu.au/15345/

Open Access Theses and Dissertations