subject:(0601 Biochemistry AND Cell Biology)

Victoria University

1. Vaughn, Michelle. Blonde hair colour : classification, characterisation and genetic associations for use in forensic science.

Degree: phd, School of Biomedical and Health Sciences, 2010, Victoria University

URL: http://vuir.vu.edu.au/15540/

► The vast variety of normal pigmentation variation is a huge genetic puzzle and hair colour is one part of this puzzle. It is hypothesised that… (more)

Subjects/Keywords: 0601 Biochemistry and Cell Biology; 0604 Genetics; School of Biomedical and Health Sciences

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APA (6^th Edition):

Vaughn, M. (2010). Blonde hair colour : classification, characterisation and genetic associations for use in forensic science. (Doctoral Dissertation). Victoria University. Retrieved from http://vuir.vu.edu.au/15540/

Chicago Manual of Style (16^th Edition):

Vaughn, Michelle. “Blonde hair colour : classification, characterisation and genetic associations for use in forensic science.” 2010. Doctoral Dissertation, Victoria University. Accessed January 25, 2020. http://vuir.vu.edu.au/15540/.

MLA Handbook (7^th Edition):

Vaughn, Michelle. “Blonde hair colour : classification, characterisation and genetic associations for use in forensic science.” 2010. Web. 25 Jan 2020.

Vancouver:

Vaughn M. Blonde hair colour : classification, characterisation and genetic associations for use in forensic science. [Internet] [Doctoral dissertation]. Victoria University; 2010. [cited 2020 Jan 25]. Available from: http://vuir.vu.edu.au/15540/.

Council of Science Editors:

Vaughn M. Blonde hair colour : classification, characterisation and genetic associations for use in forensic science. [Doctoral Dissertation]. Victoria University; 2010. Available from: http://vuir.vu.edu.au/15540/

Victoria University

2. Robinson, Ainsley. Stem cell therapies for the treatment of enteric neuropathy associated with inflammatory bowel disease.

Degree: phd, College of Health and Biomedicine, 2019, Victoria University

URL: http://vuir.vu.edu.au/39508/

► Although not associated with mortality, symptoms, complications and the relapsing nature of inflammatory bowel disease (IBD) severely impact patient’s quality of life. Current treatments are… (more)

▼ Although not associated with mortality, symptoms, complications and the relapsing nature of inflammatory bowel disease (IBD) severely impact patient’s quality of life. Current treatments are coupled with side effects and loss of patient response. Damage to the enteric neurons is consistently associated with intestinal inflammation and considered to underlie the generation of symptoms. Therefore, the enteric neurons are a potential target for novel IBD therapies. Mesenchymal stem cells (MSCs) exhibit anti-inflammatory, immunomodulating, and neuroprotective effects and are demonstrated to participate in tissue regeneration and repair in many pathological conditions. Hence, they are a viable option for the treatment of enteric neuropathy associated with IBD. The studies in this thesis aim to investigate the effects of MSC therapy in averting enteric neuropathy in acute and chronic models of IBD. The results of our studies demonstrated that MSC and conditioned medium attenuated inflammation and averted enteric neuropathy and colonic dysmotility in an acute model of IBD. The effects of MSC treatment are dose-dependent and occur as early as 24h post treatment. We characterized changes to colonic innervation, motility, transit time, microbiota and metabolome in the Winnie mouse model of spontaneously occurring chronic colitis. Our results demonstrated that the Winnie mouse is highly representative of human IBD. The mechanisms underlying colonic dysmotility in Winnie mice were due to inhibition of neuromuscular transmission and smooth muscle responses. We found that multiple high dose MSC treatments induce anti-inflammatory and neurotrophic effects in mice with chronic colitis. Single dose and multiple low dose MSC administrations were ineffective in this model. Overall, we have established the capacity of MSC treatments to attenuate inflammation and enteric neuropathy in acute and chronic models of IBD. These findings are both novel and highly relevant for clinical translation and future investigations of MSC therapy for the treatment of IBD.

Subjects/Keywords: 0601 Biochemistry and Cell Biology; 1103 Clinical Sciences; 1107 Immunology; College of Health and Biomedicine

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APA (6^th Edition):

Robinson, A. (2019). Stem cell therapies for the treatment of enteric neuropathy associated with inflammatory bowel disease. (Doctoral Dissertation). Victoria University. Retrieved from http://vuir.vu.edu.au/39508/

Chicago Manual of Style (16^th Edition):

Robinson, Ainsley. “Stem cell therapies for the treatment of enteric neuropathy associated with inflammatory bowel disease.” 2019. Doctoral Dissertation, Victoria University. Accessed January 25, 2020. http://vuir.vu.edu.au/39508/.

MLA Handbook (7^th Edition):

Robinson, Ainsley. “Stem cell therapies for the treatment of enteric neuropathy associated with inflammatory bowel disease.” 2019. Web. 25 Jan 2020.

Vancouver:

Robinson A. Stem cell therapies for the treatment of enteric neuropathy associated with inflammatory bowel disease. [Internet] [Doctoral dissertation]. Victoria University; 2019. [cited 2020 Jan 25]. Available from: http://vuir.vu.edu.au/39508/.

Council of Science Editors:

Robinson A. Stem cell therapies for the treatment of enteric neuropathy associated with inflammatory bowel disease. [Doctoral Dissertation]. Victoria University; 2019. Available from: http://vuir.vu.edu.au/39508/

University of Queensland

3. Mutemi, Kevin Nzumbi. Investigating the role of Dscam2 in the Drosophila mushroom bodies.

Degree: School of Medicine, 2018, University of Queensland

URL: https://espace.library.uq.edu.au/view/UQ:c5233ae/s4262593_masters_thesis.pdf ; https://espace.library.uq.edu.au/view/UQ:c5233ae

► This thesis is an attempt at investigating the role that Drosophila melanogaster(hereafter referred to as Drosophila) Down syndrome cell adhesion molecule 2(Dscam2) plays in mushroom… (more)

Subjects/Keywords: Drosophila; Dscam2; Dendrite; Development; Mushroom bodies; Localisation; Isoforms; 0601 Biochemistry and Cell Biology; 0604 Genetics

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APA (6^th Edition):

Mutemi, K. N. (2018). Investigating the role of Dscam2 in the Drosophila mushroom bodies. (Thesis). University of Queensland. Retrieved from https://espace.library.uq.edu.au/view/UQ:c5233ae/s4262593_masters_thesis.pdf ; https://espace.library.uq.edu.au/view/UQ:c5233ae

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mutemi, Kevin Nzumbi. “Investigating the role of Dscam2 in the Drosophila mushroom bodies.” 2018. Thesis, University of Queensland. Accessed January 25, 2020. https://espace.library.uq.edu.au/view/UQ:c5233ae/s4262593_masters_thesis.pdf ; https://espace.library.uq.edu.au/view/UQ:c5233ae.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mutemi, Kevin Nzumbi. “Investigating the role of Dscam2 in the Drosophila mushroom bodies.” 2018. Web. 25 Jan 2020.

Vancouver:

Mutemi KN. Investigating the role of Dscam2 in the Drosophila mushroom bodies. [Internet] [Thesis]. University of Queensland; 2018. [cited 2020 Jan 25]. Available from: https://espace.library.uq.edu.au/view/UQ:c5233ae/s4262593_masters_thesis.pdf ; https://espace.library.uq.edu.au/view/UQ:c5233ae.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mutemi KN. Investigating the role of Dscam2 in the Drosophila mushroom bodies. [Thesis]. University of Queensland; 2018. Available from: https://espace.library.uq.edu.au/view/UQ:c5233ae/s4262593_masters_thesis.pdf ; https://espace.library.uq.edu.au/view/UQ:c5233ae

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Victoria University

4. Smith, Renee Melissa. The Role Of Putative Nox Inhibitors In Homocysteine-Induced Vascular Dysfunction.

Degree: phd, Institute for Health and Sport, 2018, Victoria University

URL: http://vuir.vu.edu.au/39496/

► Excess plasma homocysteine (Hcy; hyperhomocysteinemia, HHcy) remains an independent risk factor for cardiovascular disease (CVD) and treatments remain elusive. The source of Hcy, methionine, is… (more)

▼ Excess plasma homocysteine (Hcy; hyperhomocysteinemia, HHcy) remains an independent risk factor for cardiovascular disease (CVD) and treatments remain elusive. The source of Hcy, methionine, is an essential amino acid acquired by ingestion of animal foods. Normal methionine metabolism effectively removes Hcy via recycling back into methionine or excretion via the kidney. However, in aberrant methionine metabolism, Hcy accumulates and causes damage to the vascular system by increasing oxidative stress; the exact mechanism of how this occurs is unknown. Importantly, the B vitamins B6, B9 and B12 are essential to proper methionine/Hcy metabolism and are often found in low levels in patients presenting with HHcy; this has provided a potentially viable treatment strategy in the clinical setting. Disappointingly, clinical trials administering B vitamins to reduce HHcy have been unsuccessful in reducing CVD and treatments continue to be sought. The NADPH oxidase (Nox) family of enzymes are expressed in a broad range of cell types throughout the body and are the primary source of superoxide (Nox1, Nox2) and hydrogen peroxide (Nox4) within the vasculature under both physiological and pathological conditions. Nox1, 2 and 4 are of primary interest in vascular disease, as there is evidence that Hcy can interfere with the proper function of Nox1, 2 and 4 signalling, potentially leading to an over-expression of pro-oxidants. Nox1, 2 and 4 have been implicated in vascular disease (endothelial dysfunction), hypertension, vascular inflammation, stroke, diabetes, and atherosclerosis, and putative inhibitors of these enzymes are now available. Additionally, nitric oxide (NO) is also measured as a marker of proper vascular function; indeed, the current gold standard of assessing NO availability is by indirectly measuring vascular responses to acetylcholine. Accordingly, a loss of NO bioavailability is linked to the development of many of the same vascular pathologies caused by HHcy and also potentially increased Nox1, 2, and 4 activity. Thus, this thesis examined if current putative Nox inhibitors could prevent vascular dysfunction caused by homocysteine (as indirectly measured by acetylcholine-mediated vasorelaxation). Using New Zealand white rabbits, C57BL/6 mice and a Nox2-/- (C57BL/6 background) mouse models, we observed that pharmacological intervention with single Nox1, 2 and 4 inhibitors reduced the effect of acetylcholine on vasorelaxation. In 1% methionine-fed Nox2-/- mice, we observed an improvement in function. We also assessed combinations of Nox1, 2 and 4 inhibitors and found that, although function was not restored to control levels, it was improved compared with single Nox inhibition. Due to these results, we performed a gp91ds-tat dose response in rabbit aorta. We found that in our models of vascular dysfunction, lower doses of gp91ds-tat significantly improved acetylcholine-mediated vasorelaxation. These results showed for the first time that in both pharmacological and diet-induced HHcy, high dose putative Nox…

Subjects/Keywords: 0601 Biochemistry and Cell Biology; 1101 Medical Biochemistry and Metabolomics; 1102 Cardiorespiratory Medicine and Haematology; Institute for Health and Sport

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APA (6^th Edition):

Smith, R. M. (2018). The Role Of Putative Nox Inhibitors In Homocysteine-Induced Vascular Dysfunction. (Doctoral Dissertation). Victoria University. Retrieved from http://vuir.vu.edu.au/39496/

Chicago Manual of Style (16^th Edition):

Smith, Renee Melissa. “The Role Of Putative Nox Inhibitors In Homocysteine-Induced Vascular Dysfunction.” 2018. Doctoral Dissertation, Victoria University. Accessed January 25, 2020. http://vuir.vu.edu.au/39496/.

MLA Handbook (7^th Edition):

Smith, Renee Melissa. “The Role Of Putative Nox Inhibitors In Homocysteine-Induced Vascular Dysfunction.” 2018. Web. 25 Jan 2020.

Vancouver:

Smith RM. The Role Of Putative Nox Inhibitors In Homocysteine-Induced Vascular Dysfunction. [Internet] [Doctoral dissertation]. Victoria University; 2018. [cited 2020 Jan 25]. Available from: http://vuir.vu.edu.au/39496/.

Council of Science Editors:

Smith RM. The Role Of Putative Nox Inhibitors In Homocysteine-Induced Vascular Dysfunction. [Doctoral Dissertation]. Victoria University; 2018. Available from: http://vuir.vu.edu.au/39496/

University of Queensland

5. Bokhari, Fawzi Faisal A. siRNA screening of the kinome identified Aurora A kinase as a therapeutic target gene in cervical cancer.

Degree: UQ Diamantina Institute, 2014, University of Queensland

URL: http://espace.library.uq.edu.au/view/UQ:341649

► HPV oncogenes disable a number of tumour suppressor pathways, including p53 and Rb, contributing to the transformed phenotype. Loss of these critical host cell functions… (more)

▼ HPV oncogenes disable a number of tumour suppressor pathways, including p53 and Rb, contributing to the transformed phenotype. Loss of these critical host cell functions may also provide an opportunity to selectively target the destruction of HPV-transformed cells. We have performed an siRNA screen using the kinome (779 genes) library to identify genes that when depleted are synthetically lethal with HPV transformation. The primary and validations screens have confirmed Aurora A kinase (AURKA) as a potential synthetic lethal target selective for HPV transformed cells. AURKA has been further investigated using the selective small molecule inhibitor MLN8237. We found that MLN8237 was significantly more potent towards the HPV transformed cells. The effect was not a consequence of targeting mitosis as two other mitotic inhibitors, PLK1 inhibitor (BI2536) and taxol, demonstrated no selectivity. Analysis of the nuclear structure and DNA content showed that Aurora A inhibition promoted a high level of polyploidy in non-HPV treated cells whilst this same degree of polyploidy was associate with apoptosis in the HPV-transformed cell lines. Whereas Bcl-2 over expression in HeLa cells had no effect on sensitivity to MLN8237, Mcl-1 overexpressing HeLa cells were less sensitive to the MLN8237 in comparison to the parental cell line, which may suggests the involvement of Noxa or Puma pro-apoptotic proteins in the induction of the apoptosis in the HPV-transformed cells. The transfection of the non-HPV C33A cervical cancer and SCC25 squamous cell carcinoma cell lines with the HPV16 oncogenic E7 increased sensitivity to MLN8237 between 3 >10 fold suggesting that the sensitivity to MLN8237-dependent killing was a direct consequence of HPV E7 expression. Xenograft experiments with cervical cancer cell lines in immunodeficient mice showed MLN8237 inhibited growth of HPV and non-HPV xenografts during treatment with 30mg/kg MLN8237 once a day for 10 consecutive days. However, outgrowth of tumour was noticed from the second day post-treatment in the non-HPV tumour group whereas the HPV-induced tumour group did not show cancer recurrence for 50 days post-treatment. These findings suggest that MLN8237 represent a promising novel therapeutic targeted agent against HPV-transformed cervical cancer.

Subjects/Keywords: siRNA; AURKA; AURKB; Haspin; GSG2; cervical cancer; HPV; synthetic lethality; 0601 Biochemistry and Cell Biology; 0604 Genetics; 1112 Oncology and Carcinogenesis

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APA (6^th Edition):

Bokhari, F. F. A. (2014). siRNA screening of the kinome identified Aurora A kinase as a therapeutic target gene in cervical cancer. (Thesis). University of Queensland. Retrieved from http://espace.library.uq.edu.au/view/UQ:341649

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bokhari, Fawzi Faisal A. “siRNA screening of the kinome identified Aurora A kinase as a therapeutic target gene in cervical cancer.” 2014. Thesis, University of Queensland. Accessed January 25, 2020. http://espace.library.uq.edu.au/view/UQ:341649.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bokhari, Fawzi Faisal A. “siRNA screening of the kinome identified Aurora A kinase as a therapeutic target gene in cervical cancer.” 2014. Web. 25 Jan 2020.

Vancouver:

Bokhari FFA. siRNA screening of the kinome identified Aurora A kinase as a therapeutic target gene in cervical cancer. [Internet] [Thesis]. University of Queensland; 2014. [cited 2020 Jan 25]. Available from: http://espace.library.uq.edu.au/view/UQ:341649.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bokhari FFA. siRNA screening of the kinome identified Aurora A kinase as a therapeutic target gene in cervical cancer. [Thesis]. University of Queensland; 2014. Available from: http://espace.library.uq.edu.au/view/UQ:341649

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Queensland

6. Sullivan, Mitchell. Liver-glycogen metabolism: A structural perspective.

Degree: Queensland Alliance for Agricultural and Food Innovation, 2014, University of Queensland

URL: http://espace.library.uq.edu.au/view/UQ:347324

► Liver glycogen, a highly branched glucose polymer, has a critical role in the maintenance of blood glucose homeostasis. Liver glycogen consists of glucose units that… (more)

▼ Liver glycogen, a highly branched glucose polymer, has a critical role in the maintenance of blood glucose homeostasis. Liver glycogen consists of glucose units that are attached to form linear chains via alpha-(1→4) linkages. These chains are connected via alpha-(1→6)-linked branch points to form highly branched glycogen “beta” particles (~20 nm in diameter) that can further join to form much larger “alpha” particles (~100-200 nm). Given the characteristically poor blood-glucose control associated with type 2 diabetes, a link between the structure/function relationships of liver glycogen and type 2 diabetes is probable. It is shown that diabetic (db/db) mice have an impaired ability to synthesize the large composite glycogen alpha particles present in normal, healthy mice and that alpha particles are held together via a bond more acid labile than normal glycosidic linkages, with the most likely bond being proteinaceous. The structure of healthy mouse liver glycogen over the diurnal cycle is characterized using size exclusion chromatography and transmission electron microscopy. Glycogen is observed to be initially formed as smaller beta particles, only being assembled into the larger alpha particles significantly after the time when glycogen content reaches a maximum. This pathway, impaired in diabetic animals, is likely to give optimal blood-glucose control, as explained by the particles’ surface area to volume ratio. Lack of this control may result from, or contribute to, the poor glycaemic regulation associated with diabetes. This discovery suggests novel approaches to diabetes management that promote alpha particle formation. Significant improvements in the extraction and characterization of liver glycogen has also been achieved, paving the way for future experiments exploring glycogen’s role in diabetes. Glycogen can now be effectively and rapidly extracted from formalin-fixed tissues using a novel technique, allowing the analysis of human tissue samples from pathology laboratories that routinely employ this method of fixation. The use of aqueous size exclusion chromatography has been shown to dramatically improve peak resolution when compared to the previously used dimethyl sulfoxide method, achieving separation of alpha-particle and beta-particle peaks. This allows for a more detailed and quantitative analysis and comparison between liver glycogen samples.

Subjects/Keywords: Glycogen; Size exclusion chromatography; Glycaemic control; Structural characterization; Diabetes; Alpha particle; 0306 Physical Chemistry (incl. Structural); 0601 Biochemistry and Cell Biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sullivan, M. (2014). Liver-glycogen metabolism: A structural perspective. (Thesis). University of Queensland. Retrieved from http://espace.library.uq.edu.au/view/UQ:347324

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sullivan, Mitchell. “Liver-glycogen metabolism: A structural perspective.” 2014. Thesis, University of Queensland. Accessed January 25, 2020. http://espace.library.uq.edu.au/view/UQ:347324.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sullivan, Mitchell. “Liver-glycogen metabolism: A structural perspective.” 2014. Web. 25 Jan 2020.

Vancouver:

Sullivan M. Liver-glycogen metabolism: A structural perspective. [Internet] [Thesis]. University of Queensland; 2014. [cited 2020 Jan 25]. Available from: http://espace.library.uq.edu.au/view/UQ:347324.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sullivan M. Liver-glycogen metabolism: A structural perspective. [Thesis]. University of Queensland; 2014. Available from: http://espace.library.uq.edu.au/view/UQ:347324

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Victoria University

7. O'Connell, Brett. A study of rat skeletal muscle Troponin C isoforms.

Degree: phd, School of Biomedical Sciences, 2005, Victoria University

URL: http://vuir.vu.edu.au/15657/

► The investigations described in this thesis were prompted by an overall interest in the phenomenon of Troponin C (TnC) polymorphism in mammalian skeletal muscle. Gaining… (more)

Subjects/Keywords: 0601 Biochemistry and Cell Biology; 1101 Medical Biochemistry and Metabolomics; 0608 Zoology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

O'Connell, B. (2005). A study of rat skeletal muscle Troponin C isoforms. (Doctoral Dissertation). Victoria University. Retrieved from http://vuir.vu.edu.au/15657/

Chicago Manual of Style (16^th Edition):

O'Connell, Brett. “A study of rat skeletal muscle Troponin C isoforms.” 2005. Doctoral Dissertation, Victoria University. Accessed January 25, 2020. http://vuir.vu.edu.au/15657/.

MLA Handbook (7^th Edition):

O'Connell, Brett. “A study of rat skeletal muscle Troponin C isoforms.” 2005. Web. 25 Jan 2020.

Vancouver:

O'Connell B. A study of rat skeletal muscle Troponin C isoforms. [Internet] [Doctoral dissertation]. Victoria University; 2005. [cited 2020 Jan 25]. Available from: http://vuir.vu.edu.au/15657/.

Council of Science Editors:

O'Connell B. A study of rat skeletal muscle Troponin C isoforms. [Doctoral Dissertation]. Victoria University; 2005. Available from: http://vuir.vu.edu.au/15657/

University of Pennsylvania

8. Dawicki McKenna, Jennine. Investigating the Role of Microtubules in Glut4 Vesicle Trafficking and the Kinetics of Membrane Attachment by the Myosin Myo1c.

Degree: 2012, University of Pennsylvania

URL: https://repository.upenn.edu/edissertations/501

► The myosin myo1c dynamically localizes to cellular membranes through high affinity phosphoinositide binding and links them to the actin cytoskeleton. Determining the kinetics of membrane… (more)

▼ The myosin myo1c dynamically localizes to cellular membranes through high affinity phosphoinositide binding and links them to the actin cytoskeleton. Determining the kinetics of membrane attachment will provide insight into the relationship between membrane-attachment and actin-attachment lifetimes, and will also provide details about the regulation of membrane attachment. Stopped-flow spectroscopy was used to measure the binding and dissociation of a recombinant myo1c construct containing the tail and regulatory domains (myo1cIQ-tail) to and from 100 nm diameter large unilamellar vesicles (LUVs). The apparent second-order rate constant for association of myo1cIQ-tail with LUVs containing 2% phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) was approximately diffusion-limited. Myo1cIQ-tail dissociated from PtdIns(4,5)P2 at a slower rate (2.0 s-1) than the pleckstrin homology domain of phospholipase C-δ (PLCδ-PH) (13 s-1). The presence of additional anionic phospholipid reduced the myo1cIQ-tail dissociation rate constant > 50-fold, but marginally changed the dissociation rate of PLCδ-PH, suggesting that additional electrostatic interactions in myo1cIQ-tail help to stabilize binding. Remarkably, high concentrations of soluble inositol phosphates induce dissociation of myo1cIQ-tail from LUVs, suggesting that phosphoinositides are able to bind and dissociate from myo1cIQ-tail as it remains bound to the membrane. In adipocytes, vesicles containing glucose transporter-4 (GLUT4) redistribute from intracellular stores to the cell periphery in response to insulin. Vesicles then fuse with the plasma membrane, facilitating glucose transport into the cell. To gain insight into the molecular role of microtubules, we examined the spatial organization and dynamics of microtubules in relation to GLUT4 vesicle trafficking in living 3T3-L1 adipocytes using total internal reflection fluorescence (TIRF) microscopy. Insulin stimulated an increase in microtubule density and curvature within the TIRF-illuminated region of the cell. The time course of the density increase precedes that of the increase in intensity of HA-GLUT4-eGFP in this same region. Microtubule disruption delayed and modestly reduced the accumulation of GLUT4 at the plasma membrane. Interestingly, fusion of GLUT4-containing vesicles with the plasma membrane preferentially occur near microtubules, and long-distance vesicle movement along microtubules visible at the cell surface prior to fusion does not appear to account for this proximity. We conclude that microtubules may be important in providing spatial information for fusion events.

Subjects/Keywords: Biochemistry; Cell Biology

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APA (6^th Edition):

Dawicki McKenna, J. (2012). Investigating the Role of Microtubules in Glut4 Vesicle Trafficking and the Kinetics of Membrane Attachment by the Myosin Myo1c. (Thesis). University of Pennsylvania. Retrieved from https://repository.upenn.edu/edissertations/501

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dawicki McKenna, Jennine. “Investigating the Role of Microtubules in Glut4 Vesicle Trafficking and the Kinetics of Membrane Attachment by the Myosin Myo1c.” 2012. Thesis, University of Pennsylvania. Accessed January 25, 2020. https://repository.upenn.edu/edissertations/501.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dawicki McKenna, Jennine. “Investigating the Role of Microtubules in Glut4 Vesicle Trafficking and the Kinetics of Membrane Attachment by the Myosin Myo1c.” 2012. Web. 25 Jan 2020.

Vancouver:

Dawicki McKenna J. Investigating the Role of Microtubules in Glut4 Vesicle Trafficking and the Kinetics of Membrane Attachment by the Myosin Myo1c. [Internet] [Thesis]. University of Pennsylvania; 2012. [cited 2020 Jan 25]. Available from: https://repository.upenn.edu/edissertations/501.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dawicki McKenna J. Investigating the Role of Microtubules in Glut4 Vesicle Trafficking and the Kinetics of Membrane Attachment by the Myosin Myo1c. [Thesis]. University of Pennsylvania; 2012. Available from: https://repository.upenn.edu/edissertations/501

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

9. Gandhi, Disha M. Strategies for the Modulation of Protease Activated Receptors (PARs).

Degree: 2018, Marquette University

URL: https://epublications.marquette.edu/dissertations_mu/802

► Protease-activated receptors (PARs) are class A G protein-coupled receptors (GPCRs) with 4 subtypes (PAR 1 – 4) and with a unique mode of action. PARs… (more)

▼ Protease-activated receptors (PARs) are class A G protein-coupled receptors (GPCRs) with 4 subtypes (PAR 1 – 4) and with a unique mode of action. PARs are cleaved by extracellular proteases at the N-terminus, creating a new tethered ligand that activates the receptor and transduces biological signals into the cell. PARs have been implicated in various productive and pathological signals, including those related to thrombosis, inflammation, reperfusion injury, and cancer cell metastasis. Despite the fact that PARs are attractive as drug targets, their intramolecular mode of activation makes it challenging to modulate them with drugs in a selective manner, and only one PAR-targeting therapy, the PAR1 antagonist and antiplatelet drug vorapaxar, is currently approved by the FDA. Previously in the Dockendorff lab, compounds with a 1,3-diaminobenzene scaffold, called parmodulins, were identified as biased ligands of PAR1 that selectively inhibit Gαq-mediated signaling of PAR1 without significantly inhibiting Gα12/13-mediated signaling leading to platelet shape change. More recently, cytoprotective and anti-inflammatory effects of parmodulins in endothelial cells were identified that could be fruitful for the treatment of sepsis and myocardial infarction. We developed a Gαq-mediated intracellular Ca2+ mobilization (iCa2+) assay in endothelial cells to perform medium-throughput screening and characterization of parmodulins, and we demonstrated that they are reversible, negative allosteric modulators of PAR1, unlike ligands such as vorapaxar. We also extended structure-activity relationship (SAR) studies of parmodulins to identify analogues with potentially improved properties using medicinal chemistry, and these parmodulins are promising leads for safer antithrombotic and sepsis drugs. Additionally, we commenced investigations into the role of heteromeric PAR complexes in proliferative disorders such as restenosis and cancer cell metastasis. We synthesized a novel class of bivalent ligands targeting putative PAR1/2 heteromers and characterized them using our iCa2+ assay with endothelial cells (EA.hy926) and the breast cancer cell line MDA-MB-231. Such bivalent PAR ligands hold promise for the selective treatment of cancer and other proliferative disorders. Advisors/Committee Members: Dockendorff, Christopher, St. Maurice, Martin, Steinmetz, Mark.

Subjects/Keywords: Biochemistry; Cell Biology

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APA (6^th Edition):

Gandhi, D. M. (2018). Strategies for the Modulation of Protease Activated Receptors (PARs). (Thesis). Marquette University. Retrieved from https://epublications.marquette.edu/dissertations_mu/802

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gandhi, Disha M. “Strategies for the Modulation of Protease Activated Receptors (PARs).” 2018. Thesis, Marquette University. Accessed January 25, 2020. https://epublications.marquette.edu/dissertations_mu/802.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gandhi, Disha M. “Strategies for the Modulation of Protease Activated Receptors (PARs).” 2018. Web. 25 Jan 2020.

Vancouver:

Gandhi DM. Strategies for the Modulation of Protease Activated Receptors (PARs). [Internet] [Thesis]. Marquette University; 2018. [cited 2020 Jan 25]. Available from: https://epublications.marquette.edu/dissertations_mu/802.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gandhi DM. Strategies for the Modulation of Protease Activated Receptors (PARs). [Thesis]. Marquette University; 2018. Available from: https://epublications.marquette.edu/dissertations_mu/802

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

10. St. Germain, Bryan J. Connecting Motors and Membranes: A Quantitative Investigation of Dynein Pathway Components and in vitro Characterization of the Num1 Coiled Coil Domain.

Degree: Master of Architecture (M.Arch.), Molecular & Cellular Biology, 2011, University of Massachusetts

URL: https://scholarworks.umass.edu/theses/720

► In the budding yeast, Saccharomyces Cerevisiae, dynein, a minus-end directed motor, is involved in nuclear migration and proper orientation of the mitotic spindle during… (more)

▼ In the budding yeast, Saccharomyces Cerevisiae, dynein, a minus-end directed motor, is involved in nuclear migration and proper orientation of the mitotic spindle during mitosis. Our lab has developed a model that involves the loading of cytoplasmic dynein onto the plus-end of astral microtubules through interactions with Pac1/LIS1 and Bik1/CLIP-170. Dynein is then delivered to the cell cortex and anchored through a cortical receptor protein, Num1. Num1 is a 313KDa protein that localizes to the cell cortex and is an essential component of dynein mediated nuclear migration. Using quantitative fluorescence techniques I was able to create a molecular inventory of various dynein pathway components. Our results revealed Dyn1, dynein heavy chain, and Pac1/LIS1 associate at the plus end in a 1:1 ratio. Additionally we found that dynein and dynactin associate in a 3:1 ratio at the plus ends and a 2:1 ratio at the cortex. Interestingly, we found that over expression of Pac1/LIS1 augments cortical dynein activity while maintaining the dynein to dynactin ratio and this activity is separate from loss of She1, a negative regulator of dynein-dynactin interaction, which results in a 1:1 ratio of dynein-dynactin at the plus-ends, as well as, the cortex. Our results uncover molecular ratios that enable us to create more defined and detailed model of the dynein pathway. To elucidate how Num1 attaches dynein to the cortex we created truncations of the Num1 protein. We were able to determine that two coiled-coil (CC) domains in the N-terminus of Num1 are responsible for bright foci formation on the cortex. Cells without these bright foci exhibit a binucleate phenotype similar to that of dyn1∆ implicating that these bright foci are required for the proper function of Num1 in the dynein pathway. To test the hypothesis that the CC is capable of mediated bright patch assembly through self-association I purified a recombinant CC domain and performed gel filtration analysis, as well as, equilibrium sedimentation. I was able to determine that the CC domain exists as a dimer in solution. However, the mechanism of CC self-assembly may involve a requisite of targeting the CC to the cortex first. Advisors/Committee Members: Wei-Lih Lee.

Subjects/Keywords: Biochemistry; Cell Biology; Molecular Biology

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APA (6^th Edition):

St. Germain, B. J. (2011). Connecting Motors and Membranes: A Quantitative Investigation of Dynein Pathway Components and in vitro Characterization of the Num1 Coiled Coil Domain. (Masters Thesis). University of Massachusetts. Retrieved from https://scholarworks.umass.edu/theses/720

Chicago Manual of Style (16^th Edition):

St. Germain, Bryan J. “Connecting Motors and Membranes: A Quantitative Investigation of Dynein Pathway Components and in vitro Characterization of the Num1 Coiled Coil Domain.” 2011. Masters Thesis, University of Massachusetts. Accessed January 25, 2020. https://scholarworks.umass.edu/theses/720.

MLA Handbook (7^th Edition):

St. Germain, Bryan J. “Connecting Motors and Membranes: A Quantitative Investigation of Dynein Pathway Components and in vitro Characterization of the Num1 Coiled Coil Domain.” 2011. Web. 25 Jan 2020.

Vancouver:

St. Germain BJ. Connecting Motors and Membranes: A Quantitative Investigation of Dynein Pathway Components and in vitro Characterization of the Num1 Coiled Coil Domain. [Internet] [Masters thesis]. University of Massachusetts; 2011. [cited 2020 Jan 25]. Available from: https://scholarworks.umass.edu/theses/720.

Council of Science Editors:

St. Germain BJ. Connecting Motors and Membranes: A Quantitative Investigation of Dynein Pathway Components and in vitro Characterization of the Num1 Coiled Coil Domain. [Masters Thesis]. University of Massachusetts; 2011. Available from: https://scholarworks.umass.edu/theses/720

University of Arkansas

11. Alghanmi, Arwa Mohammed. The Structural and Functional Properties of a Double Mutant of Human Acidic Fibroblast Growth Factor (hFGF-1).

Degree: MS, 2017, University of Arkansas

URL: https://scholarworks.uark.edu/etd/2481

► Human acidic Fibroblast Growth Factor 1 (FGF-1), a member of the FGF superfamily, is a potent mitogen and heparin-binding protein involved in a broad… (more)

Subjects/Keywords: Biochemistry; Cell Biology; Molecular Biology

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APA (6^th Edition):

Alghanmi, A. M. (2017). The Structural and Functional Properties of a Double Mutant of Human Acidic Fibroblast Growth Factor (hFGF-1). (Masters Thesis). University of Arkansas. Retrieved from https://scholarworks.uark.edu/etd/2481

Chicago Manual of Style (16^th Edition):

Alghanmi, Arwa Mohammed. “The Structural and Functional Properties of a Double Mutant of Human Acidic Fibroblast Growth Factor (hFGF-1).” 2017. Masters Thesis, University of Arkansas. Accessed January 25, 2020. https://scholarworks.uark.edu/etd/2481.

MLA Handbook (7^th Edition):

Alghanmi, Arwa Mohammed. “The Structural and Functional Properties of a Double Mutant of Human Acidic Fibroblast Growth Factor (hFGF-1).” 2017. Web. 25 Jan 2020.

Vancouver:

Alghanmi AM. The Structural and Functional Properties of a Double Mutant of Human Acidic Fibroblast Growth Factor (hFGF-1). [Internet] [Masters thesis]. University of Arkansas; 2017. [cited 2020 Jan 25]. Available from: https://scholarworks.uark.edu/etd/2481.

Council of Science Editors:

Alghanmi AM. The Structural and Functional Properties of a Double Mutant of Human Acidic Fibroblast Growth Factor (hFGF-1). [Masters Thesis]. University of Arkansas; 2017. Available from: https://scholarworks.uark.edu/etd/2481

University of Arkansas

12. ALJahdali, Nesreen Hamdan. Determination of the Effects of Different Maillard Reaction Products on the Taxonomic Composition of the Gut Microbiota.

Degree: PhD, 2017, University of Arkansas

URL: https://scholarworks.uark.edu/etd/2569

► The Maillard Reaction (MR) is a non-enzymatic chemical reaction which results in linkage between the amino group of amino acids and the carbonyl group… (more)

▼ The Maillard Reaction (MR) is a non-enzymatic chemical reaction which results in linkage between the amino group of amino acids and the carbonyl group of reduced sugars. This reaction generates Maillard reaction products (MRPs) which are not present naturally in foods, and are responsible for a range of colors, odors, flavors, and other sensory properties. Conflicting reports of MRPs impacts on human health are probably due to the fact that bioconversion of these digestible molecules by the gut microbiota has been marginally taken into account. This study aimed to determine the effects of different MRPs on rodent’s gut microbiota through16S rRNA amplicon sequencing over three different studies. Study 1 focused on the impact of NƐCarboxymethyllysine (CML) on the composition of mice gut microbiota and potential association with severity of experimental colitis. Study 2 focused on the impact of bread melanoidins on the composition of healthy and experimental colitis mice gut microbiota. Study 3 focused on the impact of consumption of increasing amounts of malt melanoidins on mice gut microbiota. It was found that CML induced limited changes in gut microbiota profiles of healthy mice, but was found to significantly relieve the bacterial dysbiosis imparted by one (but not the other) inflammation-inducing chemical, especially the Proteobacteria bloom. Bread crust model (high in melanoidins) showed significant decreases of Bacteroides spp. and Enterobacteriaceae, while it increased Faecalibacterium spp. Also, bread crust model limited to increase Enterobacteriaceae in colitis model. High amounts of malts rich melanoidins rapidly and persistently led to significantly different gut microbiota profiles. There was a trend for decrease of Lactobacillus and Ruminococcus and increase of Akkermansia and Bifidobacterium with higher amounts of dietary melanoidins. We concluded that CML and melanoidins are not detrimental in terms of their impact on the gut microbiota, and that they may even have prebiotic properties. Advisors/Committee Members: Franck Carbonero, Steve Ricke, Sami Dridi.

Subjects/Keywords: Biochemistry; Cell Biology; Molecular Biology

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APA (6^th Edition):

ALJahdali, N. H. (2017). Determination of the Effects of Different Maillard Reaction Products on the Taxonomic Composition of the Gut Microbiota. (Doctoral Dissertation). University of Arkansas. Retrieved from https://scholarworks.uark.edu/etd/2569

Chicago Manual of Style (16^th Edition):

ALJahdali, Nesreen Hamdan. “Determination of the Effects of Different Maillard Reaction Products on the Taxonomic Composition of the Gut Microbiota.” 2017. Doctoral Dissertation, University of Arkansas. Accessed January 25, 2020. https://scholarworks.uark.edu/etd/2569.

MLA Handbook (7^th Edition):

ALJahdali, Nesreen Hamdan. “Determination of the Effects of Different Maillard Reaction Products on the Taxonomic Composition of the Gut Microbiota.” 2017. Web. 25 Jan 2020.

Vancouver:

ALJahdali NH. Determination of the Effects of Different Maillard Reaction Products on the Taxonomic Composition of the Gut Microbiota. [Internet] [Doctoral dissertation]. University of Arkansas; 2017. [cited 2020 Jan 25]. Available from: https://scholarworks.uark.edu/etd/2569.

Council of Science Editors:

ALJahdali NH. Determination of the Effects of Different Maillard Reaction Products on the Taxonomic Composition of the Gut Microbiota. [Doctoral Dissertation]. University of Arkansas; 2017. Available from: https://scholarworks.uark.edu/etd/2569

University of Minnesota

13. Rust, Brittney. Exosome-Mediated Protection Against Oxidative Stress: A Connection Between NRF2 And Exosomes.

Degree: MS, Chemistry, 2016, University of Minnesota

URL: http://hdl.handle.net/11299/202070

► The nuclear factor erythroid 2 (NF-E2)-related factor 2 (NRF2) transcription factor has been widely studied for its role as the master regulator of the response… (more)

Subjects/Keywords: Biochemistry; Biology; Cell Biology

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APA (6^th Edition):

Rust, B. (2016). Exosome-Mediated Protection Against Oxidative Stress: A Connection Between NRF2 And Exosomes. (Masters Thesis). University of Minnesota. Retrieved from http://hdl.handle.net/11299/202070

Chicago Manual of Style (16^th Edition):

Rust, Brittney. “Exosome-Mediated Protection Against Oxidative Stress: A Connection Between NRF2 And Exosomes.” 2016. Masters Thesis, University of Minnesota. Accessed January 25, 2020. http://hdl.handle.net/11299/202070.

MLA Handbook (7^th Edition):

Rust, Brittney. “Exosome-Mediated Protection Against Oxidative Stress: A Connection Between NRF2 And Exosomes.” 2016. Web. 25 Jan 2020.

Vancouver:

Rust B. Exosome-Mediated Protection Against Oxidative Stress: A Connection Between NRF2 And Exosomes. [Internet] [Masters thesis]. University of Minnesota; 2016. [cited 2020 Jan 25]. Available from: http://hdl.handle.net/11299/202070.

Council of Science Editors:

Rust B. Exosome-Mediated Protection Against Oxidative Stress: A Connection Between NRF2 And Exosomes. [Masters Thesis]. University of Minnesota; 2016. Available from: http://hdl.handle.net/11299/202070

Wayne State University

14. Mcfarland, Timothy. Cardiac Calsequestrin Phosphorylation And Trafficking In The Mammalian Cardiomyocyte.

Degree: PhD, Physiology, 2011, Wayne State University

URL: https://digitalcommons.wayne.edu/oa_dissertations/176

► Cardiac CSQ (CSQ2) is a multifaceted protein, capable of binding significant quantities of Ca2+ and altering ryanodine receptor activity at the junctional sarcoplasmic reticulum… (more)

▼ Cardiac CSQ (CSQ2) is a multifaceted protein, capable of binding significant quantities of Ca2+ and altering ryanodine receptor activity at the junctional sarcoplasmic reticulum (SR). Little is known about the trafficking of CSQ2 from its unknown site of biosynthesis, which appears to be of importance as its structure changes in a trafficking-dependent manner in various types of heart failure. Through the use of multiple antibodies specific to classic rough ER markers, and with the creation of CSQ-DsRed tetramer fusion protein, we were able to establish a juxtanuclear localization of rough ER in cardiomyocytes. Using fluorescence confocal microscopy, the translocon complex proteins TRAP-α and TRAM, along with the ribosomal protein S6, were all visualized and found to encapsulate both myonuclei. Additionally, time course studies of CSQ-DsRed, in conjunction with anti-DsRed immunostaining, highlighted a perinuclear rough ER site of biosynthesis for CSQ2. The fusion protein exhibited a tetramerization-dependent trafficking predicted to be very similar to CSQ2 polymerization-dependent trafficking with high and low secretory compartment Ca2+ concentrations leading to polymerization and monomerization, respectively. Cardiac-specific C-terminal phosphorylation of CSQ2 serines was shown to effect CSQ2 trafficking according to mass spectrometric analysis of the protein's N-linked glycan. A Ser 378,382,386 Ala mutation (CSQ-nonPP) of these sites inhibited CSQ2 phosphorylation and led to an increased trafficking out of the ER as indicated by the increased mannose trimming of CSQ2 glycan. This effect was reversed with the phosphomimetic mutant Ser 378,382,386 Glu (CSQ-mimPP) which had glycoforms with mannose trimming nearly identical to that of CSQ-WT. Pharmacological and molecular inhibition were used to establish the identity of CSQ2 kinase as protein kinase CK2. In vitro, inhibition of CSQ2 phosphorylation using cardiomyocyte and nonmuscle cell CSQ2 kinase sources was successfully carried out with three specific inhibitors of CK2: TBB, DMAT and TBCA. TBCA produced identical inhibition curves for both cellular kinase sources and commercial CK2α′ kinase, with increasing concentrations of drug. SiRNA knockdown of both CK2α and CK2α′ catalytic subunits in nonmuscle cells reduced CSQ2 kinase activity by nearly 2-fold according to similar in vitro CSQ2 phosphorylation assays. Additionally, both ATP-binding site competitive inhibition and CK2 RNAi protein inhibition were used successfully in situ to suppress endogenous CSQ2 phosphorylation. TBCA used at 100 µM with CSQ-WT overexpressing cardiomyocytes and COS nonmuscle cells in culture led to an 82 and 66% decrease in CSQ2 phosphate incorporation, respectively, compared to control. Similarly, simultaneous knockdown of both CK2α and CK2α′ catalytic subunits in COS cells overexpressing adenoviral CSQ-WT, caused a near 2-fold decrease in CSQ2… Advisors/Committee Members: Steven Cala.

Subjects/Keywords: Biochemistry; Cell Biology; Molecular Biology

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APA (6^th Edition):

Mcfarland, T. (2011). Cardiac Calsequestrin Phosphorylation And Trafficking In The Mammalian Cardiomyocyte. (Doctoral Dissertation). Wayne State University. Retrieved from https://digitalcommons.wayne.edu/oa_dissertations/176

Chicago Manual of Style (16^th Edition):

Mcfarland, Timothy. “Cardiac Calsequestrin Phosphorylation And Trafficking In The Mammalian Cardiomyocyte.” 2011. Doctoral Dissertation, Wayne State University. Accessed January 25, 2020. https://digitalcommons.wayne.edu/oa_dissertations/176.

MLA Handbook (7^th Edition):

Mcfarland, Timothy. “Cardiac Calsequestrin Phosphorylation And Trafficking In The Mammalian Cardiomyocyte.” 2011. Web. 25 Jan 2020.

Vancouver:

Mcfarland T. Cardiac Calsequestrin Phosphorylation And Trafficking In The Mammalian Cardiomyocyte. [Internet] [Doctoral dissertation]. Wayne State University; 2011. [cited 2020 Jan 25]. Available from: https://digitalcommons.wayne.edu/oa_dissertations/176.

Council of Science Editors:

Mcfarland T. Cardiac Calsequestrin Phosphorylation And Trafficking In The Mammalian Cardiomyocyte. [Doctoral Dissertation]. Wayne State University; 2011. Available from: https://digitalcommons.wayne.edu/oa_dissertations/176

University of Nevada – Las Vegas

15. Villoso, Adelbert Mark. Production, purification and crystallization of membrane integrated multimeric Bax.

Degree: MSin Biological Sciences, Life Sciences, 2010, University of Nevada – Las Vegas

URL: https://digitalscholarship.unlv.edu/thesesdissertations/668

► Apoptosis, or programmed cell death, is a vital process intimately involved in the embryonic development and homeostatic maintenance of all multicellular organisms. The committing… (more)

Subjects/Keywords: Biochemistry; Cell Biology; Molecular Biology

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APA (6^th Edition):

Villoso, A. M. (2010). Production, purification and crystallization of membrane integrated multimeric Bax. (Masters Thesis). University of Nevada – Las Vegas. Retrieved from https://digitalscholarship.unlv.edu/thesesdissertations/668

Chicago Manual of Style (16^th Edition):

Villoso, Adelbert Mark. “Production, purification and crystallization of membrane integrated multimeric Bax.” 2010. Masters Thesis, University of Nevada – Las Vegas. Accessed January 25, 2020. https://digitalscholarship.unlv.edu/thesesdissertations/668.

MLA Handbook (7^th Edition):

Villoso, Adelbert Mark. “Production, purification and crystallization of membrane integrated multimeric Bax.” 2010. Web. 25 Jan 2020.

Vancouver:

Villoso AM. Production, purification and crystallization of membrane integrated multimeric Bax. [Internet] [Masters thesis]. University of Nevada – Las Vegas; 2010. [cited 2020 Jan 25]. Available from: https://digitalscholarship.unlv.edu/thesesdissertations/668.

Council of Science Editors:

Villoso AM. Production, purification and crystallization of membrane integrated multimeric Bax. [Masters Thesis]. University of Nevada – Las Vegas; 2010. Available from: https://digitalscholarship.unlv.edu/thesesdissertations/668

16. Soo, Rochelle Melissa. In search of non-photosynthetic Cyanobacteria.

Degree: School of Chemistry and Molecular Biosciences, 2015, University of Queensland

URL: http://espace.library.uq.edu.au/view/UQ:368958

Subjects/Keywords: Melainabacteria; Cyanobacteria; Metabolism; 0601 Biochemistry and Cell Biology; 0605 Microbiology

…Microbiology, 70% FoR code: 0601, Biochemistry and cell biology, 30% viii Table of Contents… …Cell Metabolism, 30% Fields of Research (FoR) Classification FoR code: 0605… …68 3.4.3 Cell shape and envelope… …91 4.4.4 Cell wall and shape… …103 4.4.10.7 Cell membrane adaptations…

10 more images…

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APA (6^th Edition):

Soo, R. M. (2015). In search of non-photosynthetic Cyanobacteria. (Thesis). University of Queensland. Retrieved from http://espace.library.uq.edu.au/view/UQ:368958

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Soo, Rochelle Melissa. “In search of non-photosynthetic Cyanobacteria.” 2015. Thesis, University of Queensland. Accessed January 25, 2020. http://espace.library.uq.edu.au/view/UQ:368958.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Soo, Rochelle Melissa. “In search of non-photosynthetic Cyanobacteria.” 2015. Web. 25 Jan 2020.

Vancouver:

Soo RM. In search of non-photosynthetic Cyanobacteria. [Internet] [Thesis]. University of Queensland; 2015. [cited 2020 Jan 25]. Available from: http://espace.library.uq.edu.au/view/UQ:368958.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Soo RM. In search of non-photosynthetic Cyanobacteria. [Thesis]. University of Queensland; 2015. Available from: http://espace.library.uq.edu.au/view/UQ:368958

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Victoria University of Technology

17. Nguyen, Long Thanh. A study of the role of glycogen in skeletal muscle performance and of myosin heavy chain isoform expression in amphibian skeletal muscle using the toad Bufo marinus.

Degree: phd, School of Life Sciences and Technology, 2000, Victoria University of Technology

URL: http://vuir.vu.edu.au/15310/

► The overall aim of this study was to contribute knowledge to two areas of inquiry in muscle research: one concerned with the molecular mechanism(s) underlying… (more)

Subjects/Keywords: 0606 Physiology; 0601 Biochemistry and Cell Biology; School of Engineering and Science

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APA (6^th Edition):

Nguyen, L. T. (2000). A study of the role of glycogen in skeletal muscle performance and of myosin heavy chain isoform expression in amphibian skeletal muscle using the toad Bufo marinus. (Doctoral Dissertation). Victoria University of Technology. Retrieved from http://vuir.vu.edu.au/15310/

Chicago Manual of Style (16^th Edition):

Nguyen, Long Thanh. “A study of the role of glycogen in skeletal muscle performance and of myosin heavy chain isoform expression in amphibian skeletal muscle using the toad Bufo marinus.” 2000. Doctoral Dissertation, Victoria University of Technology. Accessed January 25, 2020. http://vuir.vu.edu.au/15310/.

MLA Handbook (7^th Edition):

Nguyen, Long Thanh. “A study of the role of glycogen in skeletal muscle performance and of myosin heavy chain isoform expression in amphibian skeletal muscle using the toad Bufo marinus.” 2000. Web. 25 Jan 2020.

Vancouver:

Nguyen LT. A study of the role of glycogen in skeletal muscle performance and of myosin heavy chain isoform expression in amphibian skeletal muscle using the toad Bufo marinus. [Internet] [Doctoral dissertation]. Victoria University of Technology; 2000. [cited 2020 Jan 25]. Available from: http://vuir.vu.edu.au/15310/.

Council of Science Editors:

Nguyen LT. A study of the role of glycogen in skeletal muscle performance and of myosin heavy chain isoform expression in amphibian skeletal muscle using the toad Bufo marinus. [Doctoral Dissertation]. Victoria University of Technology; 2000. Available from: http://vuir.vu.edu.au/15310/

Victoria University of Technology

18. Jang, Ki-Hyo. Identification and characterisation of the cell surface and enzymatic barriers to plasmid transformation in Corynebacterium glutamicum and related species.

Degree: phd, Department of Biological and Food Technology, 1998, Victoria University of Technology

URL: http://vuir.vu.edu.au/15345/

► Non-pathogenic corynebacteria including Corynebacterium glutamicum, Brevibacterium flavum, and B. lactofermentum have been used traditionally for industrial production of amino acids. Recently, these strains have been… (more)

Subjects/Keywords: 0601 Biochemistry and Cell Biology; School of Engineering and Science

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APA (6^th Edition):

Jang, K. (1998). Identification and characterisation of the cell surface and enzymatic barriers to plasmid transformation in Corynebacterium glutamicum and related species. (Doctoral Dissertation). Victoria University of Technology. Retrieved from http://vuir.vu.edu.au/15345/

Chicago Manual of Style (16^th Edition):

Jang, Ki-Hyo. “Identification and characterisation of the cell surface and enzymatic barriers to plasmid transformation in Corynebacterium glutamicum and related species.” 1998. Doctoral Dissertation, Victoria University of Technology. Accessed January 25, 2020. http://vuir.vu.edu.au/15345/.

MLA Handbook (7^th Edition):

Jang, Ki-Hyo. “Identification and characterisation of the cell surface and enzymatic barriers to plasmid transformation in Corynebacterium glutamicum and related species.” 1998. Web. 25 Jan 2020.

Vancouver:

Jang K. Identification and characterisation of the cell surface and enzymatic barriers to plasmid transformation in Corynebacterium glutamicum and related species. [Internet] [Doctoral dissertation]. Victoria University of Technology; 1998. [cited 2020 Jan 25]. Available from: http://vuir.vu.edu.au/15345/.

Council of Science Editors:

Jang K. Identification and characterisation of the cell surface and enzymatic barriers to plasmid transformation in Corynebacterium glutamicum and related species. [Doctoral Dissertation]. Victoria University of Technology; 1998. Available from: http://vuir.vu.edu.au/15345/

Victoria University of Technology

19. Yuriev, Elizabeth. Steric aspects of the interaction of metal species with nucleic acid constituents.

Degree: phd, Department of Chemical Sciences, 1997, Victoria University of Technology

URL: http://vuir.vu.edu.au/15393/

► The delineation of steric effects in the interaction of metal species with biomolecules has been addressed. More specifically, a focus has been placed on the… (more)

Subjects/Keywords: 0305 Organic Chemistry; 0601 Biochemistry and Cell Biology; School of Engineering and Science

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APA (6^th Edition):

Yuriev, E. (1997). Steric aspects of the interaction of metal species with nucleic acid constituents. (Doctoral Dissertation). Victoria University of Technology. Retrieved from http://vuir.vu.edu.au/15393/

Chicago Manual of Style (16^th Edition):

Yuriev, Elizabeth. “Steric aspects of the interaction of metal species with nucleic acid constituents.” 1997. Doctoral Dissertation, Victoria University of Technology. Accessed January 25, 2020. http://vuir.vu.edu.au/15393/.

MLA Handbook (7^th Edition):

Yuriev, Elizabeth. “Steric aspects of the interaction of metal species with nucleic acid constituents.” 1997. Web. 25 Jan 2020.

Vancouver:

Yuriev E. Steric aspects of the interaction of metal species with nucleic acid constituents. [Internet] [Doctoral dissertation]. Victoria University of Technology; 1997. [cited 2020 Jan 25]. Available from: http://vuir.vu.edu.au/15393/.

Council of Science Editors:

Yuriev E. Steric aspects of the interaction of metal species with nucleic acid constituents. [Doctoral Dissertation]. Victoria University of Technology; 1997. Available from: http://vuir.vu.edu.au/15393/

Victoria University of Technology

20. Bhaskaracharya, Raman K. The texture and microstructure of mozzarella cheese as affected by fat content, EPS producing starter culture and fat replacers.

Degree: School of Life Sciences and Technology, 2000, Victoria University of Technology

URL: http://vuir.vu.edu.au/15412/

► The main goal of this study was to manufacture low fat mozzarella cheeses using fat replacers having similar texture and microstructure characteristics as full fat… (more)

Subjects/Keywords: 0908 Food Sciences; 0601 Biochemistry and Cell Biology; School of Engineering and Science

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APA (6^th Edition):

Bhaskaracharya, R. K. (2000). The texture and microstructure of mozzarella cheese as affected by fat content, EPS producing starter culture and fat replacers. (Masters Thesis). Victoria University of Technology. Retrieved from http://vuir.vu.edu.au/15412/

Chicago Manual of Style (16^th Edition):

Bhaskaracharya, Raman K. “The texture and microstructure of mozzarella cheese as affected by fat content, EPS producing starter culture and fat replacers.” 2000. Masters Thesis, Victoria University of Technology. Accessed January 25, 2020. http://vuir.vu.edu.au/15412/.

MLA Handbook (7^th Edition):

Bhaskaracharya, Raman K. “The texture and microstructure of mozzarella cheese as affected by fat content, EPS producing starter culture and fat replacers.” 2000. Web. 25 Jan 2020.

Vancouver:

Bhaskaracharya RK. The texture and microstructure of mozzarella cheese as affected by fat content, EPS producing starter culture and fat replacers. [Internet] [Masters thesis]. Victoria University of Technology; 2000. [cited 2020 Jan 25]. Available from: http://vuir.vu.edu.au/15412/.

Council of Science Editors:

Bhaskaracharya RK. The texture and microstructure of mozzarella cheese as affected by fat content, EPS producing starter culture and fat replacers. [Masters Thesis]. Victoria University of Technology; 2000. Available from: http://vuir.vu.edu.au/15412/

Victoria University of Technology

21. Chen, Guangyu. Studies on extracellular polysaccharide-degrading enzymes secreted by Pleurotus ostreatus grown on lupin hull.

Degree: Centre for Bioprocessing and Food Technology, 1996, Victoria University of Technology

URL: http://vuir.vu.edu.au/15418/

► Lupin hull is a waste resulting from the processing of the lupin seeds and it contains mainly polysaccharides, which is unusable by human and stock… (more)

Subjects/Keywords: 0908 Food Sciences; 0601 Biochemistry and Cell Biology; School of Engineering and Science

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APA (6^th Edition):

Chen, G. (1996). Studies on extracellular polysaccharide-degrading enzymes secreted by Pleurotus ostreatus grown on lupin hull. (Masters Thesis). Victoria University of Technology. Retrieved from http://vuir.vu.edu.au/15418/

Chicago Manual of Style (16^th Edition):

Chen, Guangyu. “Studies on extracellular polysaccharide-degrading enzymes secreted by Pleurotus ostreatus grown on lupin hull.” 1996. Masters Thesis, Victoria University of Technology. Accessed January 25, 2020. http://vuir.vu.edu.au/15418/.

MLA Handbook (7^th Edition):

Chen, Guangyu. “Studies on extracellular polysaccharide-degrading enzymes secreted by Pleurotus ostreatus grown on lupin hull.” 1996. Web. 25 Jan 2020.

Vancouver:

Chen G. Studies on extracellular polysaccharide-degrading enzymes secreted by Pleurotus ostreatus grown on lupin hull. [Internet] [Masters thesis]. Victoria University of Technology; 1996. [cited 2020 Jan 25]. Available from: http://vuir.vu.edu.au/15418/.

Council of Science Editors:

Chen G. Studies on extracellular polysaccharide-degrading enzymes secreted by Pleurotus ostreatus grown on lupin hull. [Masters Thesis]. Victoria University of Technology; 1996. Available from: http://vuir.vu.edu.au/15418/

Victoria University of Technology

22. Bortolotto, Susan Kate. A study of biochemical and physiological properties of normal and functionally impaired skeletal muscles.

Degree: phd, School of Life Science and Technology, 2000, Victoria University of Technology

URL: http://vuir.vu.edu.au/15442/

► The main aim of this study was to increase our understanding of the diversity and plasticity of skeletal muscle by using (i) normal and functionally… (more)

Subjects/Keywords: 0606 Physiology; 0601 Biochemistry and Cell Biology; School of Biomedical and Health Sciences

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APA (6^th Edition):

Bortolotto, S. K. (2000). A study of biochemical and physiological properties of normal and functionally impaired skeletal muscles. (Doctoral Dissertation). Victoria University of Technology. Retrieved from http://vuir.vu.edu.au/15442/

Chicago Manual of Style (16^th Edition):

Bortolotto, Susan Kate. “A study of biochemical and physiological properties of normal and functionally impaired skeletal muscles.” 2000. Doctoral Dissertation, Victoria University of Technology. Accessed January 25, 2020. http://vuir.vu.edu.au/15442/.

MLA Handbook (7^th Edition):

Bortolotto, Susan Kate. “A study of biochemical and physiological properties of normal and functionally impaired skeletal muscles.” 2000. Web. 25 Jan 2020.

Vancouver:

Bortolotto SK. A study of biochemical and physiological properties of normal and functionally impaired skeletal muscles. [Internet] [Doctoral dissertation]. Victoria University of Technology; 2000. [cited 2020 Jan 25]. Available from: http://vuir.vu.edu.au/15442/.

Council of Science Editors:

Bortolotto SK. A study of biochemical and physiological properties of normal and functionally impaired skeletal muscles. [Doctoral Dissertation]. Victoria University of Technology; 2000. Available from: http://vuir.vu.edu.au/15442/

Victoria University

23. Kutyna, Dariusz Roman. Isolation of low ethanol producing yeast strains using adaptive evolution.

Degree: phd, School of Molecular Sciences, 2008, Victoria University

URL: http://vuir.vu.edu.au/15484/

► This thesis describes the application of a non-genetic engineering approach, adaptive evolution, to generate variants of Saccharomyces cerevisiae that produce reduced levels of ethanol relative… (more)

Subjects/Keywords: 0908 Food Sciences; 0601 Biochemistry and Cell Biology; School of Engineering and Science

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APA (6^th Edition):

Kutyna, D. R. (2008). Isolation of low ethanol producing yeast strains using adaptive evolution. (Doctoral Dissertation). Victoria University. Retrieved from http://vuir.vu.edu.au/15484/

Chicago Manual of Style (16^th Edition):

Kutyna, Dariusz Roman. “Isolation of low ethanol producing yeast strains using adaptive evolution.” 2008. Doctoral Dissertation, Victoria University. Accessed January 25, 2020. http://vuir.vu.edu.au/15484/.

MLA Handbook (7^th Edition):

Kutyna, Dariusz Roman. “Isolation of low ethanol producing yeast strains using adaptive evolution.” 2008. Web. 25 Jan 2020.

Vancouver:

Kutyna DR. Isolation of low ethanol producing yeast strains using adaptive evolution. [Internet] [Doctoral dissertation]. Victoria University; 2008. [cited 2020 Jan 25]. Available from: http://vuir.vu.edu.au/15484/.

Council of Science Editors:

Kutyna DR. Isolation of low ethanol producing yeast strains using adaptive evolution. [Doctoral Dissertation]. Victoria University; 2008. Available from: http://vuir.vu.edu.au/15484/

Victoria University

24. Hewakapuge, Sudinna Kulangana. Prediction of age from DNA.

Degree: phd, School of Biomedical Sciences, 2009, Victoria University

URL: http://vuir.vu.edu.au/15226/

► Currently DNA profiling methods only compare a suspect’s DNA with DNA left at the crime scene. When there is no suspect, it would be useful… (more)

Subjects/Keywords: 0601 Biochemistry and Cell Biology; 0604 Genetics; School of Biomedical and Health Sciences

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APA (6^th Edition):

Hewakapuge, S. K. (2009). Prediction of age from DNA. (Doctoral Dissertation). Victoria University. Retrieved from http://vuir.vu.edu.au/15226/

Chicago Manual of Style (16^th Edition):

Hewakapuge, Sudinna Kulangana. “Prediction of age from DNA.” 2009. Doctoral Dissertation, Victoria University. Accessed January 25, 2020. http://vuir.vu.edu.au/15226/.

MLA Handbook (7^th Edition):

Hewakapuge, Sudinna Kulangana. “Prediction of age from DNA.” 2009. Web. 25 Jan 2020.

Vancouver:

Hewakapuge SK. Prediction of age from DNA. [Internet] [Doctoral dissertation]. Victoria University; 2009. [cited 2020 Jan 25]. Available from: http://vuir.vu.edu.au/15226/.

Council of Science Editors:

Hewakapuge SK. Prediction of age from DNA. [Doctoral Dissertation]. Victoria University; 2009. Available from: http://vuir.vu.edu.au/15226/

University of Oxford

25. Upcher, William R. Biochemical studies of the cohesin complex.

Degree: PhD, 2012, University of Oxford

URL: http://ora.ox.ac.uk/objects/uuid:e9a77a9c-40a5-466d-a894-c7fefeddef52 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.604377

► The accurate inheritance of genetic material depends upon the establishment and maintenance of sister chromatid cohesion. Replicated chromosomes are topologically encircled by the large, tripartite… (more)

▼ The accurate inheritance of genetic material depends upon the establishment and maintenance of sister chromatid cohesion. Replicated chromosomes are topologically encircled by the large, tripartite protein complex cohesin, allowing bi-orientation in mitosis. To entrap and reversibly dissociate from DNA, the annular complex structure must be disrupted at either the hinge domain between Smc1 and Smc3, or the interfaces created by the kleisin subunit Scc1 bridging the two ABC-like ATPase domains. The aim of this work was to characterise the cohesin complex loading and releasing mechanisms by examining the biochemical requirements for these processes. Although the identity of a chromosomal cofactor could not be assigned, the loading reaction was found to necessitate engagement of ATPase domains in an ATP-dependent manner. Notions of allosteric modulation of ATP binding and NBD engagement by acetylation were discredited. Likewise, a direct and stable physical association of hinge domains with NBDs was shown to be an unlikely conformational intermediate in a reaction thought to promote hinge opening for loading of cohesin onto DNA. The Smc3-Scc1 kleisin interface might be exploited during the opposing process of release of cohesin from DNA. Therefore, a novel protein- protein cross-linking system was adapted for use in S. cerevisiae, with a view to (1) confirming the well-founded role of hinge dissociation in topological entrapment, and (2) validating the Smc3- Scc1 interface as the recently conjectured exit gate. Despite promising preliminary kinetics in vitro, the SpyTag-SpyCatcher system was considerably less efficient in vivo. It was thus deemed unsuitable in its current format for investigating the process of interface dissociation in live cells. Finally, the large, cohesin complex-associated HEAT repeat protein Pds5 has been either speculated or shown to participate in all of the aforementioned processes, potentially modulating the hinge and Smc-kleisin interfaces. Acting as a regulatory node in cohesin function, it was pursued as an informative target for structural studies. Although no diffractive material could be obtained, Pds5 was confirmed to bind a short, N-terminal sequence of Scc1 and was in turn bound at its N- terminus by Wapl. Together, these findings contribute to defining the structures and states of the cohesin complex.

Subjects/Keywords: 572.8; Biochemistry; Biology; biology chromosome cell biochemistry

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APA (6^th Edition):

Upcher, W. R. (2012). Biochemical studies of the cohesin complex. (Doctoral Dissertation). University of Oxford. Retrieved from http://ora.ox.ac.uk/objects/uuid:e9a77a9c-40a5-466d-a894-c7fefeddef52 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.604377

Chicago Manual of Style (16^th Edition):

Upcher, William R. “Biochemical studies of the cohesin complex.” 2012. Doctoral Dissertation, University of Oxford. Accessed January 25, 2020. http://ora.ox.ac.uk/objects/uuid:e9a77a9c-40a5-466d-a894-c7fefeddef52 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.604377.

MLA Handbook (7^th Edition):

Upcher, William R. “Biochemical studies of the cohesin complex.” 2012. Web. 25 Jan 2020.

Vancouver:

Upcher WR. Biochemical studies of the cohesin complex. [Internet] [Doctoral dissertation]. University of Oxford; 2012. [cited 2020 Jan 25]. Available from: http://ora.ox.ac.uk/objects/uuid:e9a77a9c-40a5-466d-a894-c7fefeddef52 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.604377.

Council of Science Editors:

Upcher WR. Biochemical studies of the cohesin complex. [Doctoral Dissertation]. University of Oxford; 2012. Available from: http://ora.ox.ac.uk/objects/uuid:e9a77a9c-40a5-466d-a894-c7fefeddef52 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.604377

University of California, San Francisco

26. Engel, Benjamin D. Regulation of Flagellar Length by Intraflagellar Transport.

Degree: 2011, University of California, San Francisco

URL: http://pqdtopen.proquest.com/#viewpdf?dispub=3461045

► Eukaryotic flagella (also referred to as cilia) are microtubule-based organelles that protrude from the cell body into the extracellular environment. In addition to powering… (more)

▼ Eukaryotic flagella (also referred to as cilia) are microtubule-based organelles that protrude from the cell body into the extracellular environment. In addition to powering cell motility and fluid low, flagella mediate the reception of numerous signaling processes that direct developmental programs. Because flagella serve such diverse roles, defects in flagellar morphology produce a pleiotropic array of human diseases. The assembly and maintenance of flagella relies on intraflagellar transport (IFT), the molecular motor driven traffic of large protein complexes (called trains) carrying flagellar proteins between the cell body and the flagellar tip. To better understand the relationship between flagellar length and IFT, we developed a method to quantify IFT in the flagella of Chlamydomonas reinhardtii using total internal reflection fluorescence microscopy (TIRF, described in detail in chapter 2). In chapter 1, we measured changes in anterograde (base to tip) IFT during flagellar regeneration and found that IFT train size is inversely proportional to flagellar length, and may thus determine the steady state length of flagella. In chapter 3, we performed a pharmacological screen for impaired cell motility in Chlamydomonas and identified a small molecule that reduces flagellar length in several organisms, while affecting both IFT and actin dynamics. In chapter 4, we performed a genetic screen for impaired Chlamydomonas cell motility and isolated a temperature-sensitive mutant in the heavy chain of IFT dynein, the retrograde motor that returns IFT trains from the flagellar tip to the cell body. Unlike mutations in the anterograde kinesin motor, which impede both flagellar assembly and maintenance, the dynein mutant (named dhc1b-3) experiences defects in assembly but not maintenance despite significant reductions in both dynein protein and IFT activity. This surprising observation has forced us to rethink the relationship between IFT and the control of flagellar length.

Subjects/Keywords: Biology, Cell; Chemistry, Biochemistry

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APA (6^th Edition):

Engel, B. D. (2011). Regulation of Flagellar Length by Intraflagellar Transport. (Thesis). University of California, San Francisco. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=3461045

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Engel, Benjamin D. “Regulation of Flagellar Length by Intraflagellar Transport.” 2011. Thesis, University of California, San Francisco. Accessed January 25, 2020. http://pqdtopen.proquest.com/#viewpdf?dispub=3461045.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Engel, Benjamin D. “Regulation of Flagellar Length by Intraflagellar Transport.” 2011. Web. 25 Jan 2020.

Vancouver:

Engel BD. Regulation of Flagellar Length by Intraflagellar Transport. [Internet] [Thesis]. University of California, San Francisco; 2011. [cited 2020 Jan 25]. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3461045.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Engel BD. Regulation of Flagellar Length by Intraflagellar Transport. [Thesis]. University of California, San Francisco; 2011. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3461045

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California, San Francisco

27. Millius, Arthur. WAVE-mediated Actin Assembly.

Degree: 2011, University of California, San Francisco

URL: http://pqdtopen.proquest.com/#viewpdf?dispub=3474952

► This thesis presents work toward understanding the biophysical mechanisms that control polarized cell movement. Many cells move in response to external cues, and this… (more)

Subjects/Keywords: Biology, Cell; Chemistry, Biochemistry

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APA (6^th Edition):

Millius, A. (2011). WAVE-mediated Actin Assembly. (Thesis). University of California, San Francisco. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=3474952

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Millius, Arthur. “WAVE-mediated Actin Assembly.” 2011. Thesis, University of California, San Francisco. Accessed January 25, 2020. http://pqdtopen.proquest.com/#viewpdf?dispub=3474952.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Millius, Arthur. “WAVE-mediated Actin Assembly.” 2011. Web. 25 Jan 2020.

Vancouver:

Millius A. WAVE-mediated Actin Assembly. [Internet] [Thesis]. University of California, San Francisco; 2011. [cited 2020 Jan 25]. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3474952.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Millius A. WAVE-mediated Actin Assembly. [Thesis]. University of California, San Francisco; 2011. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3474952

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

28. Goodman, Benjamin. Lamin B3 antagonizes the mitotic kinesin EG5 to restrain microtubule movements during spindle assembly.

Degree: 2012, The Johns Hopkins University

URL: http://pqdtopen.proquest.com/#viewpdf?dispub=3497135

► Mitosis ensures the segregation of chromosomes to daughter cells by way of the mitotic spindle, which is composed of microtubules, microtubule associated factors, and… (more)

Subjects/Keywords: Biology, Cell; Chemistry, Biochemistry

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APA (6^th Edition):

Goodman, B. (2012). Lamin B3 antagonizes the mitotic kinesin EG5 to restrain microtubule movements during spindle assembly. (Thesis). The Johns Hopkins University. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=3497135

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Goodman, Benjamin. “Lamin B3 antagonizes the mitotic kinesin EG5 to restrain microtubule movements during spindle assembly.” 2012. Thesis, The Johns Hopkins University. Accessed January 25, 2020. http://pqdtopen.proquest.com/#viewpdf?dispub=3497135.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Goodman, Benjamin. “Lamin B3 antagonizes the mitotic kinesin EG5 to restrain microtubule movements during spindle assembly.” 2012. Web. 25 Jan 2020.

Vancouver:

Goodman B. Lamin B3 antagonizes the mitotic kinesin EG5 to restrain microtubule movements during spindle assembly. [Internet] [Thesis]. The Johns Hopkins University; 2012. [cited 2020 Jan 25]. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3497135.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Goodman B. Lamin B3 antagonizes the mitotic kinesin EG5 to restrain microtubule movements during spindle assembly. [Thesis]. The Johns Hopkins University; 2012. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3497135

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

29. Holub, Justin M. Bioconjugation to functionalize peptidomimetics| Peptoid-based selective estrogen receptor modulators.

Degree: 2010, New York University

URL: http://pqdtopen.proquest.com/#viewpdf?dispub=3380284

► Oligo-N-substituted glycines (peptoids) are a class of sequence specific peptidomimetics that are notable for their facile synthesis, sequence specificity, and peptide-like architectures. Additionally, due… (more)

▼ Oligo-N-substituted glycines (peptoids) are a class of sequence specific peptidomimetics that are notable for their facile synthesis, sequence specificity, and peptide-like architectures. Additionally, due to their proteolytic resistance and enhanced cell permeability, peptoids are considered attractive candidates for therapeutics. In an effort to expand the chemical diversity and biomedical relevance of peptoids, we report here a novel technique to site-specifically functionalize linear peptoid oligomers utilizing the copper-catalyzed azide-alkyne [3+2] cycloaddition (CuAAC) reaction. Peptoid sequences including azide or alkyne-functionalized side chains were synthesized on solid-phase support and used as substrates for CuAAC. We were successful at conjugating such diverse pendant groups as fluorophores, nucleobases, metallocenes, and steroid ligands at up to six positions along the oligomer scaffold. We have also demonstrated that the triazole linkages formed from the CuAAC reaction are compatible with standard peptoid submonomer addition chemistry. This reactive orthogonality allows for multiple cycles of conjugation and oligomer extension to be performed, facilitating the generation of highly functionalized peptidomimetic oligomers outfitted with multiple heterogeneous pendant groups. This CuAAC-mediated ligation technique was used to generate novel multivalent bioactive constructs. Estradiol-peptidomimetic conjugates (EPCs) were synthesized via site-specific conjugation of 17α-ethynylestradiol to multiple sites on azide-functionalized peptoid scaffolds. Multivalent EPCs were generated in high yield and their efficacy on binding the human estrogen receptor α (hERα) was evaluated in vitro. It was determined that EPC-hERα binding avidities are enhanced when the valency of hormone ligand is increased. To monitor EPC-mediated activation of the hERα in vivo, HEK293 cells were stably transfected with plasmids coding for hERα and subsequently treated with EPCs. EPC length and valency influenced both cell uptake and receptor modulation in this system, with highest levels of hERα activation coming from divalent 18-mer and trivalent 9-mer EPCs. Non-steroidal peptidomimetic conjugates did not activate the hERα when compared to their EPC analogues, indicating that the estradiol pendant groups are primarily responsible for the bioactivity of these constructs. Given intrinsic length and valence "tunability", and the ability to modulate the hERα in living systems, EPCs may prove to be beneficial chemical tools in activating specific nuclear, and perhaps extranuclear effects of estrogens.

Subjects/Keywords: Biology, Cell; Chemistry, Biochemistry

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APA (6^th Edition):

Holub, J. M. (2010). Bioconjugation to functionalize peptidomimetics| Peptoid-based selective estrogen receptor modulators. (Thesis). New York University. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=3380284

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Holub, Justin M. “Bioconjugation to functionalize peptidomimetics| Peptoid-based selective estrogen receptor modulators.” 2010. Thesis, New York University. Accessed January 25, 2020. http://pqdtopen.proquest.com/#viewpdf?dispub=3380284.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Holub, Justin M. “Bioconjugation to functionalize peptidomimetics| Peptoid-based selective estrogen receptor modulators.” 2010. Web. 25 Jan 2020.

Vancouver:

Holub JM. Bioconjugation to functionalize peptidomimetics| Peptoid-based selective estrogen receptor modulators. [Internet] [Thesis]. New York University; 2010. [cited 2020 Jan 25]. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3380284.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Holub JM. Bioconjugation to functionalize peptidomimetics| Peptoid-based selective estrogen receptor modulators. [Thesis]. New York University; 2010. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3380284

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Michigan Technological University

30. Kiemle, Sarah Nelson. The extracellular matrix of the charophycean green algae.

Degree: 2010, Michigan Technological University

URL: http://pqdtopen.proquest.com/#viewpdf?dispub=3422645

► A comprehensive knowledge of cell wall structure and function throughout the plant kingdom is essential to understanding cell wall evolution. The fundamental understanding of… (more)

▼ A comprehensive knowledge of cell wall structure and function throughout the plant kingdom is essential to understanding cell wall evolution. The fundamental understanding of the charophycean green algal cell wall is broadening. The similarities and differences that exist between land plant and algal cell walls provide opportunities to understand plant evolution. A variety of polymers previously associated with higher plants were discovered in the charophycean green algae (CGA), including homogalacturonans, cross-linking glycans, arabinogalactan protein, β-glucans, and cellulose. The cellulose content of CGA cell walls ranged from 6% to 43%, with the higher valuescomparable to that found in the primary cell wall of land plants (20-30%). (1,3)β-glucans were found in the unicellular Chlorokybus atmophyticus, Penium margaritaceum, and Cosmarium turpini, the unbranched filamentous Klebsormidium flaccidum, and the multicellular Chara corallina. The discovery of homogalacturonan in Penium margaritaceum represents the first confirmation of land plant-type pectins in desmids and the second rigorous characterization of a pectin polymer from the charophycean algae. Homogalacturonan was also indicated from the basal species Chlorokybus atmophyticus and Klebsormidium flaccidum. There is evidence of branched pectins in Cosmarium turpini and linkage analysis suggests the presence of type I rhamnogalacturonan (RGI). Cross-linking β-glucans are associated with cellulose microfibrils during land plant cell growth, and were found in the cell wall of CGA. The evidence of mixed-linkage glucan (MLG) in the charophytes is both suprising and significant given that MLG was once thought to be specific to some grasses. The organization and structure of Cosmarium turpini and Chara corallina MLG was found to be similar to that of Equisetum spp., whereas the basal species of the CGA, Chlorokybus atmophyticus and Klebsormidium flaccidum, have unique organization of alternating of 3- and 4-linkages. The significance of this result on the evolution of the MLG synthetic pathway has yet to be determined. The extracellular matrix (ECM) of Chlorokybus atmophyticus, Klebsormidium flaccidum, and Spirogyra spp. exhibits significant biochemical diversity, ranging from distinct “land plant” polymers to polysaccharides unique to these algae. The neutral sugar composition of Chlorokybus atmophyticus hot water extract and Spirogyra extracellular polymeric substance (EPS), combined with antibody labeling results, revealed the distinct possibility of an arabinogalactan protein in these organisms. Polysaccharide analysis of Zygnematales (desmid) EPS, indicated a probable range of different EPS backbones and substitution patterns upon the core portions of the molecules. Desmid EPS is predominately composed of a complex matrix of branched, uronic acid containing polysaccharides with ester…

Subjects/Keywords: Biology, Cell; Chemistry, Biochemistry

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APA (6^th Edition):

Kiemle, S. N. (2010). The extracellular matrix of the charophycean green algae. (Thesis). Michigan Technological University. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=3422645

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kiemle, Sarah Nelson. “The extracellular matrix of the charophycean green algae.” 2010. Thesis, Michigan Technological University. Accessed January 25, 2020. http://pqdtopen.proquest.com/#viewpdf?dispub=3422645.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kiemle, Sarah Nelson. “The extracellular matrix of the charophycean green algae.” 2010. Web. 25 Jan 2020.

Vancouver:

Kiemle SN. The extracellular matrix of the charophycean green algae. [Internet] [Thesis]. Michigan Technological University; 2010. [cited 2020 Jan 25]. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3422645.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kiemle SN. The extracellular matrix of the charophycean green algae. [Thesis]. Michigan Technological University; 2010. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=3422645

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Open Access Theses and Dissertations