subject:(0410)

University of Toronto

1. Marchand-Austin, Alexandre. Bordetella pertussis: Whole Genome Sequence Analysis and its Application to Public Health Laboratory Surveillance.

Degree: 2015, University of Toronto

URL: http://hdl.handle.net/1807/70461

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Bordetella pertussis (BP) is a Gram-negative bacterium that causes respiratory infections in humans. Multi-locus sequence types (MLST), virulence factor alleles, and antimicrobial resistance markers are… (more)

Subjects/Keywords: 0410

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APA (6^th Edition):

Marchand-Austin, A. (2015). Bordetella pertussis: Whole Genome Sequence Analysis and its Application to Public Health Laboratory Surveillance. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/70461

Chicago Manual of Style (16^th Edition):

Marchand-Austin, Alexandre. “Bordetella pertussis: Whole Genome Sequence Analysis and its Application to Public Health Laboratory Surveillance.” 2015. Masters Thesis, University of Toronto. Accessed January 17, 2021. http://hdl.handle.net/1807/70461.

MLA Handbook (7^th Edition):

Marchand-Austin, Alexandre. “Bordetella pertussis: Whole Genome Sequence Analysis and its Application to Public Health Laboratory Surveillance.” 2015. Web. 17 Jan 2021.

Vancouver:

Marchand-Austin A. Bordetella pertussis: Whole Genome Sequence Analysis and its Application to Public Health Laboratory Surveillance. [Internet] [Masters thesis]. University of Toronto; 2015. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1807/70461.

Council of Science Editors:

Marchand-Austin A. Bordetella pertussis: Whole Genome Sequence Analysis and its Application to Public Health Laboratory Surveillance. [Masters Thesis]. University of Toronto; 2015. Available from: http://hdl.handle.net/1807/70461

University of Toronto

2. Kao, Wei-Chien (Andrew). Identification and Characterization of Candidate Treponema pallidum Vascular Adhesins by Heterologous Expression in the Lyme Disease Pathogen.

Degree: 2016, University of Toronto

URL: http://hdl.handle.net/1807/74946

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This thesis reports novel techniques to study the candidate adhesin of unculturable syphilis pathogen Treponema pallidum in the Lyme disease pathogen Borrelia burgdorferi. The Treponema… (more)

Subjects/Keywords: 0410

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APA (6^th Edition):

Kao, W. (. (2016). Identification and Characterization of Candidate Treponema pallidum Vascular Adhesins by Heterologous Expression in the Lyme Disease Pathogen. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/74946

Chicago Manual of Style (16^th Edition):

Kao, Wei-Chien (Andrew). “Identification and Characterization of Candidate Treponema pallidum Vascular Adhesins by Heterologous Expression in the Lyme Disease Pathogen.” 2016. Masters Thesis, University of Toronto. Accessed January 17, 2021. http://hdl.handle.net/1807/74946.

MLA Handbook (7^th Edition):

Kao, Wei-Chien (Andrew). “Identification and Characterization of Candidate Treponema pallidum Vascular Adhesins by Heterologous Expression in the Lyme Disease Pathogen.” 2016. Web. 17 Jan 2021.

Vancouver:

Kao W(. Identification and Characterization of Candidate Treponema pallidum Vascular Adhesins by Heterologous Expression in the Lyme Disease Pathogen. [Internet] [Masters thesis]. University of Toronto; 2016. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1807/74946.

Council of Science Editors:

Kao W(. Identification and Characterization of Candidate Treponema pallidum Vascular Adhesins by Heterologous Expression in the Lyme Disease Pathogen. [Masters Thesis]. University of Toronto; 2016. Available from: http://hdl.handle.net/1807/74946

University of Toronto

3. Beckett, Emily. The Characterization of Staphylococcal Phage Tails as an Alternative to Chemical Antibiotics.

Degree: 2012, University of Toronto

URL: http://hdl.handle.net/1807/32526

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The emergence of multidrug resistant Staphylococcus aureus strains has increased the difficulty of treating these infections. Bacteriophages can eliminate bacterial infections and some bacteriophage tails… (more)

Subjects/Keywords: 0410

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APA (6^th Edition):

Beckett, E. (2012). The Characterization of Staphylococcal Phage Tails as an Alternative to Chemical Antibiotics. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/32526

Chicago Manual of Style (16^th Edition):

Beckett, Emily. “The Characterization of Staphylococcal Phage Tails as an Alternative to Chemical Antibiotics.” 2012. Masters Thesis, University of Toronto. Accessed January 17, 2021. http://hdl.handle.net/1807/32526.

MLA Handbook (7^th Edition):

Beckett, Emily. “The Characterization of Staphylococcal Phage Tails as an Alternative to Chemical Antibiotics.” 2012. Web. 17 Jan 2021.

Vancouver:

Beckett E. The Characterization of Staphylococcal Phage Tails as an Alternative to Chemical Antibiotics. [Internet] [Masters thesis]. University of Toronto; 2012. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1807/32526.

Council of Science Editors:

Beckett E. The Characterization of Staphylococcal Phage Tails as an Alternative to Chemical Antibiotics. [Masters Thesis]. University of Toronto; 2012. Available from: http://hdl.handle.net/1807/32526

University of Toronto

4. Basharat, Sinwan. Experimental Evolution to Identify Mutations that Compensate for Loss of Xenogeneic Silencing Proteins in Salmonella Typhimurium.

Degree: 2016, University of Toronto

URL: http://hdl.handle.net/1807/92187

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Horizontal Gene Transfer (HGT) is an important process that has driven the evolution of bacteria, and has been particularly important in the development of pathogenic… (more)

Subjects/Keywords: 0410

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APA (6^th Edition):

Basharat, S. (2016). Experimental Evolution to Identify Mutations that Compensate for Loss of Xenogeneic Silencing Proteins in Salmonella Typhimurium. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/92187

Chicago Manual of Style (16^th Edition):

Basharat, Sinwan. “Experimental Evolution to Identify Mutations that Compensate for Loss of Xenogeneic Silencing Proteins in Salmonella Typhimurium.” 2016. Masters Thesis, University of Toronto. Accessed January 17, 2021. http://hdl.handle.net/1807/92187.

MLA Handbook (7^th Edition):

Basharat, Sinwan. “Experimental Evolution to Identify Mutations that Compensate for Loss of Xenogeneic Silencing Proteins in Salmonella Typhimurium.” 2016. Web. 17 Jan 2021.

Vancouver:

Basharat S. Experimental Evolution to Identify Mutations that Compensate for Loss of Xenogeneic Silencing Proteins in Salmonella Typhimurium. [Internet] [Masters thesis]. University of Toronto; 2016. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1807/92187.

Council of Science Editors:

Basharat S. Experimental Evolution to Identify Mutations that Compensate for Loss of Xenogeneic Silencing Proteins in Salmonella Typhimurium. [Masters Thesis]. University of Toronto; 2016. Available from: http://hdl.handle.net/1807/92187

University of Toronto

5. Tong, Grace. Xenogeneic Silencer MvaT Differs in its DNA Binding Mechanism from Family Members H-NS and Lsr2.

Degree: 2015, University of Toronto

URL: http://hdl.handle.net/1807/69179

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Xenogeneic silencers (H-NS, Lsr2 and MvaT) facilitate bacterial evolution through the cryptic maintenance of horizontally acquired genes, binding and transcriptionally silencing DNA that is more… (more)

Subjects/Keywords: 0410

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APA (6^th Edition):

Tong, G. (2015). Xenogeneic Silencer MvaT Differs in its DNA Binding Mechanism from Family Members H-NS and Lsr2. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/69179

Chicago Manual of Style (16^th Edition):

Tong, Grace. “Xenogeneic Silencer MvaT Differs in its DNA Binding Mechanism from Family Members H-NS and Lsr2.” 2015. Masters Thesis, University of Toronto. Accessed January 17, 2021. http://hdl.handle.net/1807/69179.

MLA Handbook (7^th Edition):

Tong, Grace. “Xenogeneic Silencer MvaT Differs in its DNA Binding Mechanism from Family Members H-NS and Lsr2.” 2015. Web. 17 Jan 2021.

Vancouver:

Tong G. Xenogeneic Silencer MvaT Differs in its DNA Binding Mechanism from Family Members H-NS and Lsr2. [Internet] [Masters thesis]. University of Toronto; 2015. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1807/69179.

Council of Science Editors:

Tong G. Xenogeneic Silencer MvaT Differs in its DNA Binding Mechanism from Family Members H-NS and Lsr2. [Masters Thesis]. University of Toronto; 2015. Available from: http://hdl.handle.net/1807/69179

University of Toronto

6. Brownlie, Emma. Using In Vitro Approaches to Study Relationships between Key Members of the Murine Gut Microbiota.

Degree: 2019, University of Toronto

URL: http://hdl.handle.net/1807/102884

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The gut microbiota comprises a diverse community of organisms that have co-evolved intricate relationships with each other, leading to its involvement in a number of… (more)

Subjects/Keywords: 0410

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APA (6^th Edition):

Brownlie, E. (2019). Using In Vitro Approaches to Study Relationships between Key Members of the Murine Gut Microbiota. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/102884

Chicago Manual of Style (16^th Edition):

Brownlie, Emma. “Using In Vitro Approaches to Study Relationships between Key Members of the Murine Gut Microbiota.” 2019. Masters Thesis, University of Toronto. Accessed January 17, 2021. http://hdl.handle.net/1807/102884.

MLA Handbook (7^th Edition):

Brownlie, Emma. “Using In Vitro Approaches to Study Relationships between Key Members of the Murine Gut Microbiota.” 2019. Web. 17 Jan 2021.

Vancouver:

Brownlie E. Using In Vitro Approaches to Study Relationships between Key Members of the Murine Gut Microbiota. [Internet] [Masters thesis]. University of Toronto; 2019. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1807/102884.

Council of Science Editors:

Brownlie E. Using In Vitro Approaches to Study Relationships between Key Members of the Murine Gut Microbiota. [Masters Thesis]. University of Toronto; 2019. Available from: http://hdl.handle.net/1807/102884

University of Toronto

7. Salamun, Samuel Gerald. Mechanism and Significance of the BMRF1-NuRD Interaction.

Degree: 2020, University of Toronto

URL: http://hdl.handle.net/1807/103405

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BMRF1 has known roles as the EBV DNA polymerase processivity factor and in activating the transcription of EBV genes. However, its roles in manipulating cellular… (more)

Subjects/Keywords: 0410

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APA (6^th Edition):

Salamun, S. G. (2020). Mechanism and Significance of the BMRF1-NuRD Interaction. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/103405

Chicago Manual of Style (16^th Edition):

Salamun, Samuel Gerald. “Mechanism and Significance of the BMRF1-NuRD Interaction.” 2020. Masters Thesis, University of Toronto. Accessed January 17, 2021. http://hdl.handle.net/1807/103405.

MLA Handbook (7^th Edition):

Salamun, Samuel Gerald. “Mechanism and Significance of the BMRF1-NuRD Interaction.” 2020. Web. 17 Jan 2021.

Vancouver:

Salamun SG. Mechanism and Significance of the BMRF1-NuRD Interaction. [Internet] [Masters thesis]. University of Toronto; 2020. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1807/103405.

Council of Science Editors:

Salamun SG. Mechanism and Significance of the BMRF1-NuRD Interaction. [Masters Thesis]. University of Toronto; 2020. Available from: http://hdl.handle.net/1807/103405

University of Toronto

8. Stanley, Sabrina. An Investigation of Bacteriophage Anti-CRISPR and Anti-CRISPR Associated Proteins.

Degree: PhD, 2018, University of Toronto

URL: http://hdl.handle.net/1807/97883

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Phage infection poses a major threat to bacterial survival. To protect themselves, bacteria have evolved numerous defence mechanisms. The best known of these systems is… (more)

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Phage infection poses a major threat to bacterial survival. To protect themselves, bacteria have evolved numerous defence mechanisms. The best known of these systems is CRISPR-Cas, which provides sequence-specific protection against phage infection. In turn, phages have evolved inhibitors of CRISPR-Cas systems known as anti-CRISPRS. Here, I investigated different aspects of anti-CRISPR biology. It was previously established that anti-CRISPRs must function during the process of infection to protect phages targeted by the P. aeruginosa CRISPR-Cas system; however, it was not known how they manifest during this process. I determined that anti-CRISPR genes are robustly expressed prior to any significant degradation of phage DNA by CRISPR-Cas machinery. This expression was tied to a powerful promoter found in association with anti-CRISPR loci. While the anti-CRISPR sequences are quite diverse, the organization of their locus is well conserved. Phages were found to carry one to three anti-CRISPR genes followed by a highly conserved helix-turn-helix motif encoding gene, known as anti-CRISPR associated 1 (aca1). I determined that aca1 encodes an essential repressor of anti-CRISPR transcription, thereby explaining its conservation. Additionally, I determined that other helix-turn-helix motif containing genes found in association with anti-CRISPR loci in diverse bacterial species also act as repressors of anti-CRISPR transcription. To date, the mechanism of action of less than half of the described anti-CRISPR protein families have been studied in detail. I have demonstrated that AcrIE3 from phage DMS3 is dual function anti-CRISPR with activities against the type I-E and type I-F CRISPR-Cas systems of P. aeruginosa. Additionally, AcrIE3 was found to inhibit both CRISPR-Cas systems by preventing target DNA binding. More work is needed to determine how AcrIE3 inhibits DNA binding. Together, these studies provide insight into the spread of anti-CRISPR operons in mobile genetic elements and into the arms race between phages and their bacterial hosts.

2019-11-19 00:00:00

Advisors/Committee Members: Davidson, Alan R, Molecular and Medical Genetics.

Subjects/Keywords: 0410

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APA (6^th Edition):

Stanley, S. (2018). An Investigation of Bacteriophage Anti-CRISPR and Anti-CRISPR Associated Proteins. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/97883

Chicago Manual of Style (16^th Edition):

Stanley, Sabrina. “An Investigation of Bacteriophage Anti-CRISPR and Anti-CRISPR Associated Proteins.” 2018. Doctoral Dissertation, University of Toronto. Accessed January 17, 2021. http://hdl.handle.net/1807/97883.

MLA Handbook (7^th Edition):

Stanley, Sabrina. “An Investigation of Bacteriophage Anti-CRISPR and Anti-CRISPR Associated Proteins.” 2018. Web. 17 Jan 2021.

Vancouver:

Stanley S. An Investigation of Bacteriophage Anti-CRISPR and Anti-CRISPR Associated Proteins. [Internet] [Doctoral dissertation]. University of Toronto; 2018. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1807/97883.

Council of Science Editors:

Stanley S. An Investigation of Bacteriophage Anti-CRISPR and Anti-CRISPR Associated Proteins. [Doctoral Dissertation]. University of Toronto; 2018. Available from: http://hdl.handle.net/1807/97883

University of Toronto

9. Wenderska, Iwona Bernadetta. Understanding the Regulation and Biological Function of the ComRS Competence Signaling System in Streptococcus mutans.

Degree: PhD, 2016, University of Toronto

URL: http://hdl.handle.net/1807/93582

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Genetic competence provides bacteria with an opportunity to increase genetic diversity by allowing the acquisition of novel traits that can confer virulence or a survival… (more)

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Genetic competence provides bacteria with an opportunity to increase genetic diversity by allowing the acquisition of novel traits that can confer virulence or a survival advantage. The oral biofilm, containing a diverse microbiota at close proximity, is an ideal environment for this form of genetic exchange. Several oral streptococci have been demonstrated to engage in DNA uptake from their environment by the process of genetic transformation. In the cariogenic organism, Streptococcus mutans, competence for transformation is regulated by the ComRS peptide signaling system, comprised of the ComR transcriptional regulator and the ComS pre-peptide required to produce the mature ComX-inducting peptide, XIP. XIP in conjunction with ComR activates the ComX alternate sigma factor critical for DNA uptake and recombination. In this study, we examined the molecular pathways and biological functions of the ComRS regulon in S. mutans. We demonstrated a novel role for ComRS in competence-associated self-lysis. Further analysis of the global transcriptome in response to XIP revealed a role for XIP in the regulation of bacteriocin genes, encoding antimicrobial peptides used by bacteria to lyse closely related species. The coordination of competence development and cell lysis may ensure availability of eDNA for DNA uptake by the competent subpopulation. Aside from cell lysis, supplementation with XIP regulated a number of virulence-associated genes, and resulted in a global transcription profile that mimicked that of the starvation-induced stringent response in S. mutans. Others have shown that genetic competence and the stringent response were regulated by the rcrRPQ operon, encoding an RcrR transcriptional regulator and the RcrPQ ABC transporters. Our work further implicated RcrRPQ in the regulation of comS expression and endogenous XIP levels. Together, our work suggests that the ComRS regulon in S. mutans exhibits a biological role beyond competence induction that includes virulence and stress adaptation.

2019-02-09 00:00:00

Advisors/Committee Members: Cvitkovitch, Dennis G, Senadheera, Dilani B, Dentistry.

Subjects/Keywords: 0410

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APA (6^th Edition):

Wenderska, I. B. (2016). Understanding the Regulation and Biological Function of the ComRS Competence Signaling System in Streptococcus mutans. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/93582

Chicago Manual of Style (16^th Edition):

Wenderska, Iwona Bernadetta. “Understanding the Regulation and Biological Function of the ComRS Competence Signaling System in Streptococcus mutans.” 2016. Doctoral Dissertation, University of Toronto. Accessed January 17, 2021. http://hdl.handle.net/1807/93582.

MLA Handbook (7^th Edition):

Wenderska, Iwona Bernadetta. “Understanding the Regulation and Biological Function of the ComRS Competence Signaling System in Streptococcus mutans.” 2016. Web. 17 Jan 2021.

Vancouver:

Wenderska IB. Understanding the Regulation and Biological Function of the ComRS Competence Signaling System in Streptococcus mutans. [Internet] [Doctoral dissertation]. University of Toronto; 2016. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1807/93582.

Council of Science Editors:

Wenderska IB. Understanding the Regulation and Biological Function of the ComRS Competence Signaling System in Streptococcus mutans. [Doctoral Dissertation]. University of Toronto; 2016. Available from: http://hdl.handle.net/1807/93582

University of Toronto

10. Liu, Jingqian. Lsr2 mediates adaptation of Mycobacterium tuberculosis to changing oxygen levels and interaction with host cells.

Degree: PhD, 2017, University of Toronto

URL: http://hdl.handle.net/1807/80759

► Mycobacterium tuberculosis (M. tb), the causative agent of tuberculosis, remains a major public health threat causing approximately 2 million deaths annually. During the course of… (more)

▼ Mycobacterium tuberculosis (M. tb), the causative agent of tuberculosis, remains a major public health threat causing approximately 2 million deaths annually. During the course of infection, the bacteria encounter a variety of host environments and host-imposed stresses. In order to establish an infection at the early stage, M. tb has adapted to replicate within phagocytic cells, especially macrophages, which are specialized to kill microbes. The surviving ability of M. tb thus involves interference with critical macrophage functions, including different forms of cell death. Apoptosis of infected macrophages is associated with diminished pathogen viability, and virulent M. tb are found to subvert apoptosis signaling pathways to foil innate defense mechanisms of the macrophage. At later stage in the majority of infected individuals, the bacteria establish an asymptomatic and latent infection with a paucibacillary state in the body. Although the mechanism controlling latency remains poorly understood, oxygen tension was shown to be intimately associated with the outcome of M. tb infection. Genetic studies have shown various metabolic reprogramming occurring in M. tb during hypoxia-induced persistence or during animal models of latency. My thesis concerns the characterization of Lsr2, an important nucleoid associated protein of mycobacteria, in M. tb infection. Previous genetic study showed that it is a global regulator of M. tb that acts primarily as a transcriptional repressor. Here using an lsr2 deletion mutant, I show that Lsr2 is required for M. tb growth and survival under hypoxia, and that the mutant has altered metabolic profile compared to WT. I also found that the mutant induces apoptosis in cultured macrophages, and that its virulence is severely attenuated in a guinea pig model of infection. Lastly, using a genetic screen method, I identified LprO, a lipoprotein of unknown function in M. tb, as an inducer of macrophage apoptosis. Taken together my work implicates Lsr2 as a central regulator of M. tb that is required for adaptation of anaerobic persistence and virulence. Advisors/Committee Members: Liu, Jun, Molecular and Medical Genetics.

Subjects/Keywords: 0410

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APA (6^th Edition):

Liu, J. (2017). Lsr2 mediates adaptation of Mycobacterium tuberculosis to changing oxygen levels and interaction with host cells. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/80759

Chicago Manual of Style (16^th Edition):

Liu, Jingqian. “Lsr2 mediates adaptation of Mycobacterium tuberculosis to changing oxygen levels and interaction with host cells.” 2017. Doctoral Dissertation, University of Toronto. Accessed January 17, 2021. http://hdl.handle.net/1807/80759.

MLA Handbook (7^th Edition):

Liu, Jingqian. “Lsr2 mediates adaptation of Mycobacterium tuberculosis to changing oxygen levels and interaction with host cells.” 2017. Web. 17 Jan 2021.

Vancouver:

Liu J. Lsr2 mediates adaptation of Mycobacterium tuberculosis to changing oxygen levels and interaction with host cells. [Internet] [Doctoral dissertation]. University of Toronto; 2017. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1807/80759.

Council of Science Editors:

Liu J. Lsr2 mediates adaptation of Mycobacterium tuberculosis to changing oxygen levels and interaction with host cells. [Doctoral Dissertation]. University of Toronto; 2017. Available from: http://hdl.handle.net/1807/80759

University of Toronto

11. Deng, Xianding Wayne. Whole-genome Based Investigation of Streptococcus pneumoniae Infections.

Degree: PhD, 2017, University of Toronto

URL: http://hdl.handle.net/1807/77448

► Streptococcus pneumoniae is a common resident of the human throat, yet is also a major cause of community-acquired pneumonia, septicemia, meningitis and otitis media. In… (more)

▼ Streptococcus pneumoniae is a common resident of the human throat, yet is also a major cause of community-acquired pneumonia, septicemia, meningitis and otitis media. In recent years, whole-genome sequencing (WGS) has been extensively used in research laboratories to study infectious pathogens. However, to date, the application of WGS in clinical laboratories is limited, especially for S. pneumoniae-associated diseases. We hypothesize that variations between the genome sequence of pneumococcal isolates can be used to monitor epidemiological spread and to reveal unique traits that define clinical disease outcome. In this thesis, I used WGS in a public health laboratory setting for rapid prediction of multi-locus sequence typing (MLST) and antimicrobial resistance, establishing good sensitivity and specificity in this context. I also used WGS to delineate outbreaks of pneumococcal diseases caused by 12F that occurred in Manitoba, Canada, which was the first reported use of WGS for tracing a pneumococcal epidemic. Through genomic comparisons of Canadian and U.S.A. 12F isolates, I was able to pinpoint the source of the outbreak, and monitor transmission and evolution of an emerging 12F outbreak strain. Finally, I performed a large-scale genome comparison of pneumococcal isolates associated with different clinical manifestations to gain insight into genomic landscape of S. pneumoniae, with a goal to reveal genetic differences between strains with a propensity to cause different types of disease. From this work, I have identified a handful of candidate genes that tend to differentiate pathogenic versus commensal strains When considered together, this thesis demonstrates the power of WGS in clinical epidemiology. Once routinely applied, WGS will allow high resolution mapping of epidemics, helpful predictions of antimicrobial resistance, and the presence of factors indicating a predisposition to cause different types of pneumococcal disease. When given the rapid turnaround of state-of-the-art sequencers, this will provide a profound benefit for clinical care and public health interventions against this incessant pathogen. Advisors/Committee Members: Mazzulli, Tony, Owen, Scott-Gray, Laboratory Medicine and Pathobiology.

Subjects/Keywords: 0410

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APA (6^th Edition):

Deng, X. W. (2017). Whole-genome Based Investigation of Streptococcus pneumoniae Infections. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/77448

Chicago Manual of Style (16^th Edition):

Deng, Xianding Wayne. “Whole-genome Based Investigation of Streptococcus pneumoniae Infections.” 2017. Doctoral Dissertation, University of Toronto. Accessed January 17, 2021. http://hdl.handle.net/1807/77448.

MLA Handbook (7^th Edition):

Deng, Xianding Wayne. “Whole-genome Based Investigation of Streptococcus pneumoniae Infections.” 2017. Web. 17 Jan 2021.

Vancouver:

Deng XW. Whole-genome Based Investigation of Streptococcus pneumoniae Infections. [Internet] [Doctoral dissertation]. University of Toronto; 2017. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1807/77448.

Council of Science Editors:

Deng XW. Whole-genome Based Investigation of Streptococcus pneumoniae Infections. [Doctoral Dissertation]. University of Toronto; 2017. Available from: http://hdl.handle.net/1807/77448

12. Hill, Jessica Anne. Determining the Mechanisms of Resistance to Drug Combinations and their Fitness Effects in Candida albicans.

Degree: PhD, 2014, University of Toronto

URL: http://hdl.handle.net/1807/68186

► The evolution of drug resistance is occurring rapidly and threatening our arsenal of antimicrobials. This is a poignant issue with antifungals, whose targets are limited… (more)

▼ The evolution of drug resistance is occurring rapidly and threatening our arsenal of antimicrobials. This is a poignant issue with antifungals, whose targets are limited due to conservation between fungi and the human host. A promising strategy is to enhance effectiveness of our current antifungals using combination therapy. Adding an inhibitor of one of the key stress response proteins, Hsp90 or calcineurin, to an antifungal azole renders the activity of the azole fungicidal and abrogates azole resistance. However, evidence from clinical isolates indicates that resistance to the drug combination can still arise. Determining the mechanisms of resistance to these drug combinations and their fitness effects will provide information about how resistance can occur and whether it is likely to spread in a population. To address what mechanisms of resistance can arise to drug combinations, I evolved strains of the leading fungal pathogen, Candida albicans, and its genetically tractable relative Saccharomyces cerevisiae, in the presence of an azole and the Hsp90 inhibitor geldanamycin (GdA) or the calcineurin inhibitor FK506. The vast majority of strains (286) went extinct, however, I identified and validated mechanisms of resistance in 13 strains. A variety of resistance mechanisms were found, from well-established resistance mechanisms to novel ones. To address the fitness effects of resistance to an azole in combination with GdA or FK506, I took advantage of these C. albicans in vitro evolved strains and a series of C. albicans clinical isolates that transition to Hsp90- and calcineurin-independent azole resistance. I determined that resistance to the drug combination is often but not universally costly in the absence of drug and in novel, clinically relevant stress environments. Together these results provide a basis for our understanding of key parameters for the evolution of resistance to drug combinations: the variety of mechanisms that encompass resistance, and their fitness costs. Advisors/Committee Members: Leah, E Cowen, Molecular and Medical Genetics.

Subjects/Keywords: 0410

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APA (6^th Edition):

Hill, J. A. (2014). Determining the Mechanisms of Resistance to Drug Combinations and their Fitness Effects in Candida albicans. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/68186

Chicago Manual of Style (16^th Edition):

Hill, Jessica Anne. “Determining the Mechanisms of Resistance to Drug Combinations and their Fitness Effects in Candida albicans.” 2014. Doctoral Dissertation, University of Toronto. Accessed January 17, 2021. http://hdl.handle.net/1807/68186.

MLA Handbook (7^th Edition):

Hill, Jessica Anne. “Determining the Mechanisms of Resistance to Drug Combinations and their Fitness Effects in Candida albicans.” 2014. Web. 17 Jan 2021.

Vancouver:

Hill JA. Determining the Mechanisms of Resistance to Drug Combinations and their Fitness Effects in Candida albicans. [Internet] [Doctoral dissertation]. University of Toronto; 2014. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1807/68186.

Council of Science Editors:

Hill JA. Determining the Mechanisms of Resistance to Drug Combinations and their Fitness Effects in Candida albicans. [Doctoral Dissertation]. University of Toronto; 2014. Available from: http://hdl.handle.net/1807/68186

University of Toronto

13. Galimanas, B. Vaia. Investigating the oral microbiome in health and periodontal disease.

Degree: 2014, University of Toronto

URL: http://hdl.handle.net/1807/68492

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16S rRNA was used to determine the microbiome associated with health and chronic periodontitis (CP). We hypothesized that a comparison of plaque in health and… (more)

Subjects/Keywords: microbiome; periodontal; 0410

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APA (6^th Edition):

Galimanas, B. V. (2014). Investigating the oral microbiome in health and periodontal disease. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/68492

Chicago Manual of Style (16^th Edition):

Galimanas, B Vaia. “Investigating the oral microbiome in health and periodontal disease.” 2014. Masters Thesis, University of Toronto. Accessed January 17, 2021. http://hdl.handle.net/1807/68492.

MLA Handbook (7^th Edition):

Galimanas, B Vaia. “Investigating the oral microbiome in health and periodontal disease.” 2014. Web. 17 Jan 2021.

Vancouver:

Galimanas BV. Investigating the oral microbiome in health and periodontal disease. [Internet] [Masters thesis]. University of Toronto; 2014. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1807/68492.

Council of Science Editors:

Galimanas BV. Investigating the oral microbiome in health and periodontal disease. [Masters Thesis]. University of Toronto; 2014. Available from: http://hdl.handle.net/1807/68492

University of Toronto

14. Forster, Carol. The Role of the Innated Immune System in Bisphonate-induced Osteonecrosis of the Jaw.

Degree: 2011, University of Toronto

URL: http://hdl.handle.net/1807/30597

►

Bisphosphonate-induced osteonecrosis of the jaw (BPONJ) has been identified as a severe complication of dental treatment in 1-10% of patients previously treated with intravenous bisphosphonates.… (more)

Subjects/Keywords: Neutrophil; Bisphosphonate; 0410

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APA (6^th Edition):

Forster, C. (2011). The Role of the Innated Immune System in Bisphonate-induced Osteonecrosis of the Jaw. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/30597

Chicago Manual of Style (16^th Edition):

Forster, Carol. “The Role of the Innated Immune System in Bisphonate-induced Osteonecrosis of the Jaw.” 2011. Masters Thesis, University of Toronto. Accessed January 17, 2021. http://hdl.handle.net/1807/30597.

MLA Handbook (7^th Edition):

Forster, Carol. “The Role of the Innated Immune System in Bisphonate-induced Osteonecrosis of the Jaw.” 2011. Web. 17 Jan 2021.

Vancouver:

Forster C. The Role of the Innated Immune System in Bisphonate-induced Osteonecrosis of the Jaw. [Internet] [Masters thesis]. University of Toronto; 2011. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1807/30597.

Council of Science Editors:

Forster C. The Role of the Innated Immune System in Bisphonate-induced Osteonecrosis of the Jaw. [Masters Thesis]. University of Toronto; 2011. Available from: http://hdl.handle.net/1807/30597

University of Toronto

15. Saravia, Sandy. Disruption of the Toll-like Receptor 4 Signaling Pathway by Salmonella Effector SigD.

Degree: 2011, University of Toronto

URL: http://hdl.handle.net/1807/29614

►

The enteropathogenic bacteria Salmonella are the main cause of food borne gastroenteritis worldwide. The activation of Toll-like receptor 4 (TLR4) by LPS triggers an immune… (more)

Subjects/Keywords: Salmonella; SigD; 0410

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APA (6^th Edition):

Saravia, S. (2011). Disruption of the Toll-like Receptor 4 Signaling Pathway by Salmonella Effector SigD. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/29614

Chicago Manual of Style (16^th Edition):

Saravia, Sandy. “Disruption of the Toll-like Receptor 4 Signaling Pathway by Salmonella Effector SigD.” 2011. Masters Thesis, University of Toronto. Accessed January 17, 2021. http://hdl.handle.net/1807/29614.

MLA Handbook (7^th Edition):

Saravia, Sandy. “Disruption of the Toll-like Receptor 4 Signaling Pathway by Salmonella Effector SigD.” 2011. Web. 17 Jan 2021.

Vancouver:

Saravia S. Disruption of the Toll-like Receptor 4 Signaling Pathway by Salmonella Effector SigD. [Internet] [Masters thesis]. University of Toronto; 2011. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1807/29614.

Council of Science Editors:

Saravia S. Disruption of the Toll-like Receptor 4 Signaling Pathway by Salmonella Effector SigD. [Masters Thesis]. University of Toronto; 2011. Available from: http://hdl.handle.net/1807/29614

University of Toronto

16. McSheffrey, Gordon Gerald. Host Cellular Responses to Neisseria gonorrhoeae.

Degree: 2016, University of Toronto

URL: http://hdl.handle.net/1807/71686

►

Neisseria gonorrhoeae is a human-restricted pathogen associated with increasing morbidity as antibiotic-resistant strains spread globally. N. gonorrhoeae possesses sophisticated mechanisms to survive the host immune… (more)

Subjects/Keywords: gonorrhea; macrophage; phosphoinositides; 0410

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APA (6^th Edition):

McSheffrey, G. G. (2016). Host Cellular Responses to Neisseria gonorrhoeae. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/71686

Chicago Manual of Style (16^th Edition):

McSheffrey, Gordon Gerald. “Host Cellular Responses to Neisseria gonorrhoeae.” 2016. Masters Thesis, University of Toronto. Accessed January 17, 2021. http://hdl.handle.net/1807/71686.

MLA Handbook (7^th Edition):

McSheffrey, Gordon Gerald. “Host Cellular Responses to Neisseria gonorrhoeae.” 2016. Web. 17 Jan 2021.

Vancouver:

McSheffrey GG. Host Cellular Responses to Neisseria gonorrhoeae. [Internet] [Masters thesis]. University of Toronto; 2016. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1807/71686.

Council of Science Editors:

McSheffrey GG. Host Cellular Responses to Neisseria gonorrhoeae. [Masters Thesis]. University of Toronto; 2016. Available from: http://hdl.handle.net/1807/71686

University of Toronto

17. Chiang, Jennifer. In Search of Interaction Partners for the Saccharomyces cerevisiae Magnesium Channel Alr1p.

Degree: 2011, University of Toronto

URL: http://hdl.handle.net/1807/30564

►

Magnesium, the second most abundant cation in the cell, is involved in a diverse range of biochemical activities. This project focuses on the mechanism of… (more)

Subjects/Keywords: protein interaction; magnesium channel; 0410

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APA (6^th Edition):

Chiang, J. (2011). In Search of Interaction Partners for the Saccharomyces cerevisiae Magnesium Channel Alr1p. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/30564

Chicago Manual of Style (16^th Edition):

Chiang, Jennifer. “In Search of Interaction Partners for the Saccharomyces cerevisiae Magnesium Channel Alr1p.” 2011. Masters Thesis, University of Toronto. Accessed January 17, 2021. http://hdl.handle.net/1807/30564.

MLA Handbook (7^th Edition):

Chiang, Jennifer. “In Search of Interaction Partners for the Saccharomyces cerevisiae Magnesium Channel Alr1p.” 2011. Web. 17 Jan 2021.

Vancouver:

Chiang J. In Search of Interaction Partners for the Saccharomyces cerevisiae Magnesium Channel Alr1p. [Internet] [Masters thesis]. University of Toronto; 2011. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1807/30564.

Council of Science Editors:

Chiang J. In Search of Interaction Partners for the Saccharomyces cerevisiae Magnesium Channel Alr1p. [Masters Thesis]. University of Toronto; 2011. Available from: http://hdl.handle.net/1807/30564

University of Toronto

18. Gigliozzi, Darren. Bacteria Filamentation, a Contributing Factor to the Intracellular Survival of Legionella pneumophila.

Degree: 2012, University of Toronto

URL: http://hdl.handle.net/1807/33217

►

Legionella pneumophila (Lp) is the pathogen responsible for Legionnaires disease. Lp invades and survives in human macrophages to form an intracellular compartment, called the Legionella… (more)

Subjects/Keywords: Legionella; Macrophages; 0410; 0379

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APA (6^th Edition):

Gigliozzi, D. (2012). Bacteria Filamentation, a Contributing Factor to the Intracellular Survival of Legionella pneumophila. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/33217

Chicago Manual of Style (16^th Edition):

Gigliozzi, Darren. “Bacteria Filamentation, a Contributing Factor to the Intracellular Survival of Legionella pneumophila.” 2012. Masters Thesis, University of Toronto. Accessed January 17, 2021. http://hdl.handle.net/1807/33217.

MLA Handbook (7^th Edition):

Gigliozzi, Darren. “Bacteria Filamentation, a Contributing Factor to the Intracellular Survival of Legionella pneumophila.” 2012. Web. 17 Jan 2021.

Vancouver:

Gigliozzi D. Bacteria Filamentation, a Contributing Factor to the Intracellular Survival of Legionella pneumophila. [Internet] [Masters thesis]. University of Toronto; 2012. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1807/33217.

Council of Science Editors:

Gigliozzi D. Bacteria Filamentation, a Contributing Factor to the Intracellular Survival of Legionella pneumophila. [Masters Thesis]. University of Toronto; 2012. Available from: http://hdl.handle.net/1807/33217

University of Toronto

19. Gao, Duan (Aggie). The Role of Septins during Invasion of Host Cells by Salmonella enterica serovar Typhimurium.

Degree: 2016, University of Toronto

URL: http://hdl.handle.net/1807/92572

►

Salmonella enterica serovar Typhimurium (S. Typhimurium) initiates invasion of host cells using a Type 3 Secretion System to translocate bacterial virulence proteins (known as effectors)… (more)

Subjects/Keywords: Invasion; Pathogenesis; Salmonella; Septin; 0410

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APA (6^th Edition):

Gao, D. (. (2016). The Role of Septins during Invasion of Host Cells by Salmonella enterica serovar Typhimurium. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/92572

Chicago Manual of Style (16^th Edition):

Gao, Duan (Aggie). “The Role of Septins during Invasion of Host Cells by Salmonella enterica serovar Typhimurium.” 2016. Masters Thesis, University of Toronto. Accessed January 17, 2021. http://hdl.handle.net/1807/92572.

MLA Handbook (7^th Edition):

Gao, Duan (Aggie). “The Role of Septins during Invasion of Host Cells by Salmonella enterica serovar Typhimurium.” 2016. Web. 17 Jan 2021.

Vancouver:

Gao D(. The Role of Septins during Invasion of Host Cells by Salmonella enterica serovar Typhimurium. [Internet] [Masters thesis]. University of Toronto; 2016. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1807/92572.

Council of Science Editors:

Gao D(. The Role of Septins during Invasion of Host Cells by Salmonella enterica serovar Typhimurium. [Masters Thesis]. University of Toronto; 2016. Available from: http://hdl.handle.net/1807/92572

University of Toronto

20. Stevenson, James. Hha and YdgT Act Through H-NS to Repress Horizontally Acquired Genes.

Degree: 2010, University of Toronto

URL: http://hdl.handle.net/1807/25812

►

The bacterial protein H-NS acts to silence horizontally acquired genes. H-NS physically interacts via its N-terminus with two paralogous proteins, Hha and YdgT. Deletion of… (more)

Subjects/Keywords: H-NS; Salmonella; 0307; 0410

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APA (6^th Edition):

Stevenson, J. (2010). Hha and YdgT Act Through H-NS to Repress Horizontally Acquired Genes. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/25812

Chicago Manual of Style (16^th Edition):

Stevenson, James. “Hha and YdgT Act Through H-NS to Repress Horizontally Acquired Genes.” 2010. Masters Thesis, University of Toronto. Accessed January 17, 2021. http://hdl.handle.net/1807/25812.

MLA Handbook (7^th Edition):

Stevenson, James. “Hha and YdgT Act Through H-NS to Repress Horizontally Acquired Genes.” 2010. Web. 17 Jan 2021.

Vancouver:

Stevenson J. Hha and YdgT Act Through H-NS to Repress Horizontally Acquired Genes. [Internet] [Masters thesis]. University of Toronto; 2010. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1807/25812.

Council of Science Editors:

Stevenson J. Hha and YdgT Act Through H-NS to Repress Horizontally Acquired Genes. [Masters Thesis]. University of Toronto; 2010. Available from: http://hdl.handle.net/1807/25812

University of Toronto

21. Tjia, Jason Alexander. Journey into C. albicans Biofilms: Proteomic and functional genomic approaches to uncovering mechanisms of adherence.

Degree: 2016, University of Toronto

URL: http://hdl.handle.net/1807/72808

►

Candida albicans is an opportunistic fungal human pathogen that naturally forms biofilms, which are a community of yeast and filamentous cells surrounded by an extracellular… (more)

Subjects/Keywords: Adherence; Biofilms; Candida; Endocytosis; 0410

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APA (6^th Edition):

Tjia, J. A. (2016). Journey into C. albicans Biofilms: Proteomic and functional genomic approaches to uncovering mechanisms of adherence. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/72808

Chicago Manual of Style (16^th Edition):

Tjia, Jason Alexander. “Journey into C. albicans Biofilms: Proteomic and functional genomic approaches to uncovering mechanisms of adherence.” 2016. Masters Thesis, University of Toronto. Accessed January 17, 2021. http://hdl.handle.net/1807/72808.

MLA Handbook (7^th Edition):

Tjia, Jason Alexander. “Journey into C. albicans Biofilms: Proteomic and functional genomic approaches to uncovering mechanisms of adherence.” 2016. Web. 17 Jan 2021.

Vancouver:

Tjia JA. Journey into C. albicans Biofilms: Proteomic and functional genomic approaches to uncovering mechanisms of adherence. [Internet] [Masters thesis]. University of Toronto; 2016. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1807/72808.

Council of Science Editors:

Tjia JA. Journey into C. albicans Biofilms: Proteomic and functional genomic approaches to uncovering mechanisms of adherence. [Masters Thesis]. University of Toronto; 2016. Available from: http://hdl.handle.net/1807/72808

University of Toronto

22. Lastovetska, Olga. Surveillance of Extended-spectrum Cephalosporin- and Carbapenem-resistance in Escherichia coli from the Greater Toronto Area, Ontario, Canada.

Degree: 2012, University of Toronto

URL: http://hdl.handle.net/1807/33660

►

The purpose of this study was to investigate the prevalence and mechanisms of extended-spectrum cephalosporin- (ESC) and carbapenem-resistance in Escherichia coli from the GTA. A… (more)

Subjects/Keywords: E. coli; cephalosporin-resistance; 0410

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APA (6^th Edition):

Lastovetska, O. (2012). Surveillance of Extended-spectrum Cephalosporin- and Carbapenem-resistance in Escherichia coli from the Greater Toronto Area, Ontario, Canada. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/33660

Chicago Manual of Style (16^th Edition):

Lastovetska, Olga. “Surveillance of Extended-spectrum Cephalosporin- and Carbapenem-resistance in Escherichia coli from the Greater Toronto Area, Ontario, Canada.” 2012. Masters Thesis, University of Toronto. Accessed January 17, 2021. http://hdl.handle.net/1807/33660.

MLA Handbook (7^th Edition):

Lastovetska, Olga. “Surveillance of Extended-spectrum Cephalosporin- and Carbapenem-resistance in Escherichia coli from the Greater Toronto Area, Ontario, Canada.” 2012. Web. 17 Jan 2021.

Vancouver:

Lastovetska O. Surveillance of Extended-spectrum Cephalosporin- and Carbapenem-resistance in Escherichia coli from the Greater Toronto Area, Ontario, Canada. [Internet] [Masters thesis]. University of Toronto; 2012. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1807/33660.

Council of Science Editors:

Lastovetska O. Surveillance of Extended-spectrum Cephalosporin- and Carbapenem-resistance in Escherichia coli from the Greater Toronto Area, Ontario, Canada. [Masters Thesis]. University of Toronto; 2012. Available from: http://hdl.handle.net/1807/33660

University of Toronto

23. Cardarelli, Rodilia. A Structural and Evolutionary Analysis of the Bacteriophage Head-tail Connector.

Degree: 2010, University of Toronto

URL: http://hdl.handle.net/1807/26135

►

Macromolecular complexes are important in almost all cellular processes. The bacteriophage head-tail connector complex offers a model with which to study the mechanisms that control… (more)

Subjects/Keywords: 0487; 0410

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APA (6^th Edition):

Cardarelli, R. (2010). A Structural and Evolutionary Analysis of the Bacteriophage Head-tail Connector. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/26135

Chicago Manual of Style (16^th Edition):

Cardarelli, Rodilia. “A Structural and Evolutionary Analysis of the Bacteriophage Head-tail Connector.” 2010. Doctoral Dissertation, University of Toronto. Accessed January 17, 2021. http://hdl.handle.net/1807/26135.

MLA Handbook (7^th Edition):

Cardarelli, Rodilia. “A Structural and Evolutionary Analysis of the Bacteriophage Head-tail Connector.” 2010. Web. 17 Jan 2021.

Vancouver:

Cardarelli R. A Structural and Evolutionary Analysis of the Bacteriophage Head-tail Connector. [Internet] [Doctoral dissertation]. University of Toronto; 2010. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1807/26135.

Council of Science Editors:

Cardarelli R. A Structural and Evolutionary Analysis of the Bacteriophage Head-tail Connector. [Doctoral Dissertation]. University of Toronto; 2010. Available from: http://hdl.handle.net/1807/26135

University of Toronto

24. Sintsova, Anna. CEACAM3: Specific Innate Immunity.

Degree: PhD, 2015, University of Toronto

URL: http://hdl.handle.net/1807/80191

► The bacterial species Neisseria gonorrhoeae remains one of the most prevalent bacterial causes of sexually transmitted infections. Gonorrhea is typically characterized by a painful purulent… (more)

▼ The bacterial species Neisseria gonorrhoeae remains one of the most prevalent bacterial causes of sexually transmitted infections. Gonorrhea is typically characterized by a painful purulent discharge and inflammation, the outcome of overzealous recruitment of neutrophils, phagocytic immune cells highly specialized in the detection and elimination of microbial pathogens. Although molecular mechanisms of gonococcal pathogenesis have been studied in great detail, the causes for the excessive inflammation and immunopathology that follow infection are still not clear. The human neutrophil-restricted innate immune receptor CEACAM3 has been previously shown to promote opsonin-independent uptake and killing of N. gonorrhoeae by neutrophils. In the course of my thesis work, I have established that the role of CEACAM3 is not restricted to the neutrophils’ direct engulfment and killing of gonococci, since it also drives a vigorous inflammatory response that typifies gonorrhea. By carrying the potential to mobilize increasing numbers of neutrophils, CEACAM3 represents a tipping point between the protective and pathogenic outcomes of N. gonorrhoeae infection. My biochemical profiling of gonococcal-infected, genetically modified neutrophils revealed that CEACAM3 engagement triggers a Syk-, PKCδ-, Bcl10-MALT1-, and Tak1-dependent signaling cascade that leads to the activation of an NF-κB-dependent transcriptional response, with consequent production of pro-inflammatory cytokines. I also showed that CEACAM cross-linking on neutrophils signals in synergy with TLR4, significantly amplifying the response to LPS, suggesting that CEACAM3 acts as an integral part of a complex innate immune detection network. Finally, I co-led a study that characterized Opa protein variant expression among a collection of clinical specimen-derived N. gonorrhoeae, and then assessed their CEACAM binding profile. This study suggested that mucosal infection in humans selects for binding to epithelial-expressed CEACAMs but against CEACAM3 binding in vivo. Together, my studies implicate a role for CEACAM3 as a host-adaptive innate receptor that promotes gonococcal detection and elimination in the context of natural infection, but also reveal potential pathogenic consequences of CEACAM3 activation. Advisors/Committee Members: Gray-Owen, Scott, Molecular and Medical Genetics.

Subjects/Keywords: CEACAM; Neisseria; Neutrophil; 0410

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APA (6^th Edition):

Sintsova, A. (2015). CEACAM3: Specific Innate Immunity. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/80191

Chicago Manual of Style (16^th Edition):

Sintsova, Anna. “CEACAM3: Specific Innate Immunity.” 2015. Doctoral Dissertation, University of Toronto. Accessed January 17, 2021. http://hdl.handle.net/1807/80191.

MLA Handbook (7^th Edition):

Sintsova, Anna. “CEACAM3: Specific Innate Immunity.” 2015. Web. 17 Jan 2021.

Vancouver:

Sintsova A. CEACAM3: Specific Innate Immunity. [Internet] [Doctoral dissertation]. University of Toronto; 2015. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1807/80191.

Council of Science Editors:

Sintsova A. CEACAM3: Specific Innate Immunity. [Doctoral Dissertation]. University of Toronto; 2015. Available from: http://hdl.handle.net/1807/80191

University of Toronto

25. Tsao, Yu Fan. Systematic Study of Bacterial Phenotypes Caused by Pseudomonas aeruginosa Prophage Adaptive Genes.

Degree: 2015, University of Toronto

URL: http://hdl.handle.net/1807/69722

►

In addition to being the major predator of bacteria, some bacteriophages adapted a symbiotic relationship with their bacterial hosts through the formation of prophages. Unique… (more)

Subjects/Keywords: bacteriophage; lysogenic conversion; Pseudomonas aeruginosa; 0410

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APA (6^th Edition):

Tsao, Y. F. (2015). Systematic Study of Bacterial Phenotypes Caused by Pseudomonas aeruginosa Prophage Adaptive Genes. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/69722

Chicago Manual of Style (16^th Edition):

Tsao, Yu Fan. “Systematic Study of Bacterial Phenotypes Caused by Pseudomonas aeruginosa Prophage Adaptive Genes.” 2015. Masters Thesis, University of Toronto. Accessed January 17, 2021. http://hdl.handle.net/1807/69722.

MLA Handbook (7^th Edition):

Tsao, Yu Fan. “Systematic Study of Bacterial Phenotypes Caused by Pseudomonas aeruginosa Prophage Adaptive Genes.” 2015. Web. 17 Jan 2021.

Vancouver:

Tsao YF. Systematic Study of Bacterial Phenotypes Caused by Pseudomonas aeruginosa Prophage Adaptive Genes. [Internet] [Masters thesis]. University of Toronto; 2015. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1807/69722.

Council of Science Editors:

Tsao YF. Systematic Study of Bacterial Phenotypes Caused by Pseudomonas aeruginosa Prophage Adaptive Genes. [Masters Thesis]. University of Toronto; 2015. Available from: http://hdl.handle.net/1807/69722

University of Toronto

26. Magnan, Chantal Nicole. Investigating the Determinants of Host Range Specificity of Pseudomonas aeruginosa Phage.

Degree: 2015, University of Toronto

URL: http://hdl.handle.net/1807/70489

►

Phage therapy, or the use of bacteriophages (phages) to kill pathogenic bacteria, represents an alternative strategy to antibiotics in combating bacterial disease in the face… (more)

Subjects/Keywords: aeruginosa; Bacteriophage; fibers; Pseudomonas; Specificity; tail; 0410

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APA (6^th Edition):

Magnan, C. N. (2015). Investigating the Determinants of Host Range Specificity of Pseudomonas aeruginosa Phage. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/70489

Chicago Manual of Style (16^th Edition):

Magnan, Chantal Nicole. “Investigating the Determinants of Host Range Specificity of Pseudomonas aeruginosa Phage.” 2015. Masters Thesis, University of Toronto. Accessed January 17, 2021. http://hdl.handle.net/1807/70489.

MLA Handbook (7^th Edition):

Magnan, Chantal Nicole. “Investigating the Determinants of Host Range Specificity of Pseudomonas aeruginosa Phage.” 2015. Web. 17 Jan 2021.

Vancouver:

Magnan CN. Investigating the Determinants of Host Range Specificity of Pseudomonas aeruginosa Phage. [Internet] [Masters thesis]. University of Toronto; 2015. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1807/70489.

Council of Science Editors:

Magnan CN. Investigating the Determinants of Host Range Specificity of Pseudomonas aeruginosa Phage. [Masters Thesis]. University of Toronto; 2015. Available from: http://hdl.handle.net/1807/70489

University of Toronto

27. Vaidya, Anup Shirish. Elucidating the Role of ARKL1 in Regulating Epstein-Barr Virus (EBV) Iinfection.

Degree: 2016, University of Toronto

URL: http://hdl.handle.net/1807/76190

►

ARKL1 and Arkadia were previously identified as proteins that bind to the CK2Î˛ KSSR motif. Since the Epstein-Barr virus (EBV) protein Epstein-Barr nuclear antigen 1… (more)

Subjects/Keywords: Arkadia; ARKL1; EBV; Jun; Polycomb; Reactivation; 0410

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APA (6^th Edition):

Vaidya, A. S. (2016). Elucidating the Role of ARKL1 in Regulating Epstein-Barr Virus (EBV) Iinfection. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/76190

Chicago Manual of Style (16^th Edition):

Vaidya, Anup Shirish. “Elucidating the Role of ARKL1 in Regulating Epstein-Barr Virus (EBV) Iinfection.” 2016. Masters Thesis, University of Toronto. Accessed January 17, 2021. http://hdl.handle.net/1807/76190.

MLA Handbook (7^th Edition):

Vaidya, Anup Shirish. “Elucidating the Role of ARKL1 in Regulating Epstein-Barr Virus (EBV) Iinfection.” 2016. Web. 17 Jan 2021.

Vancouver:

Vaidya AS. Elucidating the Role of ARKL1 in Regulating Epstein-Barr Virus (EBV) Iinfection. [Internet] [Masters thesis]. University of Toronto; 2016. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1807/76190.

Council of Science Editors:

Vaidya AS. Elucidating the Role of ARKL1 in Regulating Epstein-Barr Virus (EBV) Iinfection. [Masters Thesis]. University of Toronto; 2016. Available from: http://hdl.handle.net/1807/76190

University of Toronto

28. Neiman, Jana. Evolution of cps Loci in Vibrio vulnificus.

Degree: 2011, University of Toronto

URL: http://hdl.handle.net/1807/31364

►

Vibrio vulnificus is an opportunistic human and animal pathogen with the highest death rate of any foodborne disease agent. The capsular polysaccharide (CPS) is essential… (more)

Subjects/Keywords: Vibrio vulnificus; CPS; Horizontal gene transfer; 0410

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APA (6^th Edition):

Neiman, J. (2011). Evolution of cps Loci in Vibrio vulnificus. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/31364

Chicago Manual of Style (16^th Edition):

Neiman, Jana. “Evolution of cps Loci in Vibrio vulnificus.” 2011. Masters Thesis, University of Toronto. Accessed January 17, 2021. http://hdl.handle.net/1807/31364.

MLA Handbook (7^th Edition):

Neiman, Jana. “Evolution of cps Loci in Vibrio vulnificus.” 2011. Web. 17 Jan 2021.

Vancouver:

Neiman J. Evolution of cps Loci in Vibrio vulnificus. [Internet] [Masters thesis]. University of Toronto; 2011. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1807/31364.

Council of Science Editors:

Neiman J. Evolution of cps Loci in Vibrio vulnificus. [Masters Thesis]. University of Toronto; 2011. Available from: http://hdl.handle.net/1807/31364

University of Toronto

29. Li, Peter Yan. In Silico Metabolic Network Reconstruction of Scheffersomyces Stipitis.

Degree: 2012, University of Toronto

URL: http://hdl.handle.net/1807/32597

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In this study, a genome-scale metabolic model was constructed using published genomic and biochemical data. By employing the flux balance analysis (FBA) framework, the model… (more)

Subjects/Keywords: metabolic model; S. stipitis; FBA; 0542; 0410

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Li, P. Y. (2012). In Silico Metabolic Network Reconstruction of Scheffersomyces Stipitis. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/32597

Chicago Manual of Style (16^th Edition):

Li, Peter Yan. “In Silico Metabolic Network Reconstruction of Scheffersomyces Stipitis.” 2012. Masters Thesis, University of Toronto. Accessed January 17, 2021. http://hdl.handle.net/1807/32597.

MLA Handbook (7^th Edition):

Li, Peter Yan. “In Silico Metabolic Network Reconstruction of Scheffersomyces Stipitis.” 2012. Web. 17 Jan 2021.

Vancouver:

Li PY. In Silico Metabolic Network Reconstruction of Scheffersomyces Stipitis. [Internet] [Masters thesis]. University of Toronto; 2012. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1807/32597.

Council of Science Editors:

Li PY. In Silico Metabolic Network Reconstruction of Scheffersomyces Stipitis. [Masters Thesis]. University of Toronto; 2012. Available from: http://hdl.handle.net/1807/32597

University of Toronto

30. Quach, Van Chau. Optimization of Purification Conditions for the Pseudomonas syringae HopZ1a Type III Secreted Effector Protein for Structural and Functional Studies.

Degree: 2010, University of Toronto

URL: http://hdl.handle.net/1807/24278

►

HopZ1a is a type III secreted effector (TTSE) protein from Pseudomonas syringae. The goal of this study was to obtain a 3D crystal structure of… (more)

Subjects/Keywords: Molecular biology; microbiology; 0309; 0379; 0410; 0307

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Quach, V. C. (2010). Optimization of Purification Conditions for the Pseudomonas syringae HopZ1a Type III Secreted Effector Protein for Structural and Functional Studies. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/24278

Chicago Manual of Style (16^th Edition):

Quach, Van Chau. “Optimization of Purification Conditions for the Pseudomonas syringae HopZ1a Type III Secreted Effector Protein for Structural and Functional Studies.” 2010. Masters Thesis, University of Toronto. Accessed January 17, 2021. http://hdl.handle.net/1807/24278.

MLA Handbook (7^th Edition):

Quach, Van Chau. “Optimization of Purification Conditions for the Pseudomonas syringae HopZ1a Type III Secreted Effector Protein for Structural and Functional Studies.” 2010. Web. 17 Jan 2021.

Vancouver:

Quach VC. Optimization of Purification Conditions for the Pseudomonas syringae HopZ1a Type III Secreted Effector Protein for Structural and Functional Studies. [Internet] [Masters thesis]. University of Toronto; 2010. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1807/24278.

Council of Science Editors:

Quach VC. Optimization of Purification Conditions for the Pseudomonas syringae HopZ1a Type III Secreted Effector Protein for Structural and Functional Studies. [Masters Thesis]. University of Toronto; 2010. Available from: http://hdl.handle.net/1807/24278

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