subject:( molecular aspects)

Brock University

1. Boyle, Patrick. Unique and unifying themes in the mechanisms regulating the expression of the arabidopsis thaliana PR-1 and Solanum tuberosum PR-10a inducible defense genes .

Degree: Centre for Biotechnology, 2010, Brock University

URL: http://hdl.handle.net/10464/3031

► Arabidopsis is a model plant used to study disease resistance; Solanum tuberosum or potato is a crop species. Both plants possess inducible defense mechanisms that… (more)

▼ Arabidopsis is a model plant used to study disease resistance; Solanum tuberosum or potato is a crop species. Both plants possess inducible defense mechanisms that are deployed upon recognition of pathogen invasion. Transcriptional reprogramming is crucial to the activation of defense responses. The Pathogenesis-Related (PR) genes are activated in these defense programs. Expression of Arabidopsis PR-l and potato PR-10a serve as markers for the deployment of defense responses in these plants. PR-l expression indicates induction of systemic acquired resistance (SAR). Activation of SAR requires accumulation of salicylic acid (SA), in addition to the interaction of the non-expressor of pathogenesis-related genes I (NPRI), with the TGA transcription factors. The PR-10a is activated in response to pathogen invasion, wounding and elicitor treatment. PR-10a induction requires recruitment of the Whirly I (Whyl) activator to the promoter. This locus is also negatively regulated by the silencer element binding factor (SEBF). We established that both the PR-l and PR-10a are occupied by repressors under non-inducing conditions. TGA2 was found to be a constitutive resident and repressor of PR-l, which mediates repression by forming an oligomeric complex on the promoter. The DNA-binding activity of this oligomer required the TGA2 N-terminus (NT). Under resting conditions we determined that the PR-10a is bound by a repressosome containing SEBF and curiously the activator Pto interacting protein 4 (Pti4). In the context of this repressosome, SEBF is responsible for PR-10a binding, yet rWe also showed that PR-l and PR-10a are activated by different means. In PR-l activation the NPRI NT domain alleviates TGA2-mediated repression by interacting with the TGA2 NT. TGA2 remains at the PR-l but adopts a dimeric conformation and forms an enhanceosome with NPRl. In contrast, the PR-10a is activated by evicting the repressosome and recruiting Why! to the promoter. These results advance our understanding of the mechanisms regulating PR-l and PR-10a expression under resting and inducing conditions. This study also revealed that the means of regulation for related genes can differ greatly between model and crop s

Subjects/Keywords: Arabidopsis – Molecular aspects; Solanum – Molecular aspects

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APA (6^th Edition):

Boyle, P. (2010). Unique and unifying themes in the mechanisms regulating the expression of the arabidopsis thaliana PR-1 and Solanum tuberosum PR-10a inducible defense genes . (Thesis). Brock University. Retrieved from http://hdl.handle.net/10464/3031

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Boyle, Patrick. “Unique and unifying themes in the mechanisms regulating the expression of the arabidopsis thaliana PR-1 and Solanum tuberosum PR-10a inducible defense genes .” 2010. Thesis, Brock University. Accessed September 16, 2019. http://hdl.handle.net/10464/3031.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Boyle, Patrick. “Unique and unifying themes in the mechanisms regulating the expression of the arabidopsis thaliana PR-1 and Solanum tuberosum PR-10a inducible defense genes .” 2010. Web. 16 Sep 2019.

Vancouver:

Boyle P. Unique and unifying themes in the mechanisms regulating the expression of the arabidopsis thaliana PR-1 and Solanum tuberosum PR-10a inducible defense genes . [Internet] [Thesis]. Brock University; 2010. [cited 2019 Sep 16]. Available from: http://hdl.handle.net/10464/3031.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Boyle P. Unique and unifying themes in the mechanisms regulating the expression of the arabidopsis thaliana PR-1 and Solanum tuberosum PR-10a inducible defense genes . [Thesis]. Brock University; 2010. Available from: http://hdl.handle.net/10464/3031

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University

2. Codding, Sara Jone. Functional and Structural Analyses of Three Distinct proteins by Biochemical and Biophysical Techniques.

Degree: PhD, Biochemistry & Biophysics, 2016, Oregon State University

URL: http://hdl.handle.net/1957/60138

► The focus of this dissertation was to understand protein function and structure on a molecular level. To do this successfully, a variety of biochemical and… (more)

▼ The focus of this dissertation was to understand protein function and structure on a molecular level. To do this successfully, a variety of biochemical and biophysical techniques were employed. A wide variety of techniques were applied to study the proteins of interest in this dissertation to probe protein-protein interactions, protein function, protein structure, and protein regulation. In addition, the use of bioinformatics has placed the similarities and differences of these proteins compared to homologs in perspective within their protein families. There are three chapters of original research presented here, the second chapter is published, the third contains my contribution to a work that is published, and the fourth will be submitted for publication. The second chapter of this thesis describes the studies on the protein dysferlin. Mutations in dysferlin are responsible for late onset muscular dystrophies where muscle cells are deficient in resealing the cell membrane upon routine injury. We demonstrate that dysferlin catalyzes the rate-limiting step of membrane fusion and mediates lipid mixing in a calcium sensitive manner. This work was the first in the field to determine a mechanism of action of dysferlin and suggests a molecular basis for why mutations that abrogate dysferlin's activity result in muscular dystrophy. The third chapter includes my contribution to the complete structural solution of the enzyme ValA involved in the synthesis of the crop protectant validamycin A. This was the first structure of a sedoheputlose-7-phoshpate cyclase, and the overall fold reflects that of similar sugar cyclase proteins. But, the sequence and structural analysis of ValA with respect to other related proteins revealed the first novel theory regarding substrate specificity of ValA. It is postulated that due to sequence alignment and structural data, ValA selectively binds the β-anomer of its substrate which pre-assigns stereochemistry of the enzymatic product. In the fourth chapter of this dissertation we design several variants of the protein merlin involved in cell contact growth inhibition. Whereas a complete structural solution was never obtained for merlin in this study, we used in vitro techniques to test the role of merlin's central domain in the conformational regulation of the protein. This was the first study on merlin, or the homologous proteins ezrin, radixin, and moesin, that focused on the role of the central domain. Our results confirm that this domain is not merely structural, but mediates key interactions that modulate merlin's degree of intramolecular association. That merlin's activity is central to cell contact growth inhibition and that this activity is modulated by merlin's degree of intramolecular association, it is clear now that the central domain plays a key role in the activity of merlin. In the final chapter and fifth chapter I conclude this dissertation. Advisors/Committee Members: Johnson, Colin (advisor), Taylor, Barbara J (committee member).

Subjects/Keywords: dysferlin; Muscular dystrophy – Molecular aspects

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APA (6^th Edition):

Codding, S. J. (2016). Functional and Structural Analyses of Three Distinct proteins by Biochemical and Biophysical Techniques. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/60138

Chicago Manual of Style (16^th Edition):

Codding, Sara Jone. “Functional and Structural Analyses of Three Distinct proteins by Biochemical and Biophysical Techniques.” 2016. Doctoral Dissertation, Oregon State University. Accessed September 16, 2019. http://hdl.handle.net/1957/60138.

MLA Handbook (7^th Edition):

Codding, Sara Jone. “Functional and Structural Analyses of Three Distinct proteins by Biochemical and Biophysical Techniques.” 2016. Web. 16 Sep 2019.

Vancouver:

Codding SJ. Functional and Structural Analyses of Three Distinct proteins by Biochemical and Biophysical Techniques. [Internet] [Doctoral dissertation]. Oregon State University; 2016. [cited 2019 Sep 16]. Available from: http://hdl.handle.net/1957/60138.

Council of Science Editors:

Codding SJ. Functional and Structural Analyses of Three Distinct proteins by Biochemical and Biophysical Techniques. [Doctoral Dissertation]. Oregon State University; 2016. Available from: http://hdl.handle.net/1957/60138

Oregon State University

3. Creason, Allison L. Characterization of the pathogenicity and phylogeny of members of the Rhodococcus genus.

Degree: PhD, Molecular and Cellular Biology, 2015, Oregon State University

URL: http://hdl.handle.net/1957/55812

► Plant pathogenic Rhodococcus species are persistent pathogens able to cause severe growth deformities on a large range of hosts. The most well studied species, R.… (more)

Subjects/Keywords: Rhodococcus – Phylogeny – Molecular aspects

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APA (6^th Edition):

Creason, A. L. (2015). Characterization of the pathogenicity and phylogeny of members of the Rhodococcus genus. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/55812

Chicago Manual of Style (16^th Edition):

Creason, Allison L. “Characterization of the pathogenicity and phylogeny of members of the Rhodococcus genus.” 2015. Doctoral Dissertation, Oregon State University. Accessed September 16, 2019. http://hdl.handle.net/1957/55812.

MLA Handbook (7^th Edition):

Creason, Allison L. “Characterization of the pathogenicity and phylogeny of members of the Rhodococcus genus.” 2015. Web. 16 Sep 2019.

Vancouver:

Creason AL. Characterization of the pathogenicity and phylogeny of members of the Rhodococcus genus. [Internet] [Doctoral dissertation]. Oregon State University; 2015. [cited 2019 Sep 16]. Available from: http://hdl.handle.net/1957/55812.

Council of Science Editors:

Creason AL. Characterization of the pathogenicity and phylogeny of members of the Rhodococcus genus. [Doctoral Dissertation]. Oregon State University; 2015. Available from: http://hdl.handle.net/1957/55812

University of Hong Kong

4. Tey, Sze Keong. Clinical significance and functional role of nuclear met in hepatocellular carcinoma.

Degree: PhD, 2015, University of Hong Kong

URL: Tey, S. K. [鄭思強]. (2015). Clinical significance and functional role of nuclear met in hepatocellular carcinoma. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5699886 ; http://dx.doi.org/10.5353/th_b5699886 ; http://hdl.handle.net/10722/231140

► Hepatocellular carcinoma (HCC) is the fifth most prevalent cancer and the third most common cause of cancer mortality worldwide. Understanding the underlying mechanisms that contributed… (more)

▼ Hepatocellular carcinoma (HCC) is the fifth most prevalent cancer and the third most common cause of cancer mortality worldwide. Understanding the underlying mechanisms that contributed to hepatocarcinogenesis would certainly provide more effective therapeutic options as the prognosis of HCC remains poor. Met/HGF signaling, implicated in various carcinomas including HCC, is an attractive target for human cancers. Indeed, therapeutic strategies against Met surface receptor are currently in clinical trials. The presence of Met inside nucleus will undoubtedly challenge the old-school idea of receptor tyrosine kinase signaling always occurred at the membrane surface, suggesting a novel “signaling shortcut”. In this study, we attempted to provide a comprehensive understanding regarding the involvement of nuclear Met in HCC by focusing on three major goals; first, the expression and clinical relevance of nuclear Met in HCC; second, the functions of nuclear Met in HCC tumourigenesis and progression; and finally, the underlying signaling network mediated by nuclear Met. The expression of nuclear Met was examined in human HCC cell lines and tissue samples. Nuclear Met was shown to be overexpressed in HCC tumorous tissues than the corresponding non-tumorous tissues. Moreover, its expression increased progressively along HCC development, from non-tumorous liver, cirrhotic liver to early and advanced HCC. Besides, nuclear Met overexpression was associated with venous invasion and poorer overall survival in HCC patients. We then confirmed that nuclear Met, at a size of around 48 kDa, only consists of the cytoplasmic fragment of full length Met as it could only be detected by Met antibody that directed against the cytoplasmic domain but not the extracellular domain of Met. Subsequent cellular fractionation and immunofluorescence microscopy confirmed that this 48 kDa fragment of Met was present in the nucleus in a ligand-independent fashion. We also identified a region preceding the tyrosine kinase domain after juxtamembrane region (P1027-L1157) is necessary for the translocation of nuclear Met. Nuclear Met overexpressing stable clones were established to functionally characterize the nuclear Met in HCC by subjecting stable clones to various functional studies. Collectively, we demonstrated the promoting effect of nuclear Met in in vitro cell proliferation, anchorage independent growth, motility and invasiveness. Moreover, nuclear Met overexpression also enhanced the in vivo tumourigenicity of HCC cells. Our data provided the first compelling evidences of an oncogenic function of nuclear Met in HCC. To elucidate the underlying mechanism mediated by nuclear Met in promoting HCC tumourigenesis and aggressiveness, we performed luciferase reporter assay and showed the activation of NF-κB reporter by nuclear Met expression. Subsequent inhibition of IKKβ and p65 activity, two important effectors of NF-κB signaling, abolished the promoting effect of nuclear Met on HCC cells motility and invasiveness. Also, quantitative…

Subjects/Keywords: Liver - Cancer - Molecular aspects

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APA (6^th Edition):

Tey, S. K. (2015). Clinical significance and functional role of nuclear met in hepatocellular carcinoma. (Doctoral Dissertation). University of Hong Kong. Retrieved from Tey, S. K. [鄭思強]. (2015). Clinical significance and functional role of nuclear met in hepatocellular carcinoma. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5699886 ; http://dx.doi.org/10.5353/th_b5699886 ; http://hdl.handle.net/10722/231140

Chicago Manual of Style (16^th Edition):

Tey, Sze Keong. “Clinical significance and functional role of nuclear met in hepatocellular carcinoma.” 2015. Doctoral Dissertation, University of Hong Kong. Accessed September 16, 2019. Tey, S. K. [鄭思強]. (2015). Clinical significance and functional role of nuclear met in hepatocellular carcinoma. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5699886 ; http://dx.doi.org/10.5353/th_b5699886 ; http://hdl.handle.net/10722/231140.

MLA Handbook (7^th Edition):

Tey, Sze Keong. “Clinical significance and functional role of nuclear met in hepatocellular carcinoma.” 2015. Web. 16 Sep 2019.

Vancouver:

Tey SK. Clinical significance and functional role of nuclear met in hepatocellular carcinoma. [Internet] [Doctoral dissertation]. University of Hong Kong; 2015. [cited 2019 Sep 16]. Available from: Tey, S. K. [鄭思強]. (2015). Clinical significance and functional role of nuclear met in hepatocellular carcinoma. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5699886 ; http://dx.doi.org/10.5353/th_b5699886 ; http://hdl.handle.net/10722/231140.

Council of Science Editors:

Tey SK. Clinical significance and functional role of nuclear met in hepatocellular carcinoma. [Doctoral Dissertation]. University of Hong Kong; 2015. Available from: Tey, S. K. [鄭思強]. (2015). Clinical significance and functional role of nuclear met in hepatocellular carcinoma. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5699886 ; http://dx.doi.org/10.5353/th_b5699886 ; http://hdl.handle.net/10722/231140

University of Hong Kong

5. Chan, Yan-yan. The role of cyclin E1 in hepatocellular carcinoma.

Degree: M. Phil., 2014, University of Hong Kong

URL: Chan, Y. [陳茵茵]. (2014). The role of cyclin E1 in hepatocellular carcinoma. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5351033 ; http://dx.doi.org/10.5353/th_b5351033 ; http://hdl.handle.net/10722/208041

►

Hepatocellular carcinoma (HCC) accounts for 70-85% of liver cancer, which is the sixth most common cancer in the world. Prognosis of HCC is dismal with… (more)

▼

Hepatocellular carcinoma (HCC) accounts for 70-85% of liver cancer, which is the sixth most common cancer in the world. Prognosis of HCC is dismal with little chance of complete recovery after diagnosis. It is of essence to discover the key molecules involved in the tumor progression. This could help earlier detection of HCC and establish targeted molecular therapies. Cyclin E1 (CCNE1) is a cyclin molecule responsible for the transition from G1 to S phase of the cell cycle and is often dysregulated in human cancers. CCNE1 is reported with overexpression in about 30-70% of HCC cases. It expresses in tumor cells as a ladder of proteins and as low molecular weight CCNE1. The study is aimed to investigate the role of CCNE1 in HCC. From the local cohort of HCC patients, 6 out of 13 patients (46.2%) of HCC tumor tissues were found with CCNE1 overexpression compared with the non-tumor tissues by western blotting. The presence of three CCNE1 isoforms in HCC was detected. The expression of total CCNE1 and each isoform varied independently among the studied HCC cell lines, with HepG2 having the highest expression and 97L the lowest. To extend our study on the regulation of CCNE1 expression, the expression of selected four genes associating with the CCNE1 expression and functions was studied by quantitative PCR (qPCR). F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase (FBXW7) and cullin 3 (CUL3), the two genes responsible for CCNE1 degradation, had increased expression in the HCC cell lines with higher CCNE1 expression. Cyclin A (CCNA2), the downstream cyclin molecule of CCNE1, also had higher expression in these cell lines. In contrast, the expression of cyclin dependent kinase 2 (CDK2), the catalytic partner of CCNE1, had the least difference among the six HCC cell lines compared to other three genes. To characterize the role of CCNE1 isoforms in HCC, CCNE1 isoform 1, 2, and 3 were overexpressed in PLC cells and such overexpression remained even after 8 passages in culture. In flow cytometric analysis, GFP signal in cell culture population was viewed to observe the transduction efficiency. The vector control showed the strongest GFP signal, followed by CCNE1 isoform 3 showing dim signal. CCNE1 isoform 1 and 2 almost showed no signal. In the functional studies, the overexpression of CCNE1 isoform 3 could increase proliferation and migration of HCC cells. In summary, CCNE1 could promote proliferation and migration of HCC cells through elevated expression of CCNE1 isoform 3.

published_or_final_version

Surgery

Master

Master of Philosophy

Advisors/Committee Members: Lee, NPY.

Subjects/Keywords: Cyclins; Liver - Cancer - Molecular aspects

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APA (6^th Edition):

Chan, Y. (2014). The role of cyclin E1 in hepatocellular carcinoma. (Masters Thesis). University of Hong Kong. Retrieved from Chan, Y. [陳茵茵]. (2014). The role of cyclin E1 in hepatocellular carcinoma. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5351033 ; http://dx.doi.org/10.5353/th_b5351033 ; http://hdl.handle.net/10722/208041

Chicago Manual of Style (16^th Edition):

Chan, Yan-yan. “The role of cyclin E1 in hepatocellular carcinoma.” 2014. Masters Thesis, University of Hong Kong. Accessed September 16, 2019. Chan, Y. [陳茵茵]. (2014). The role of cyclin E1 in hepatocellular carcinoma. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5351033 ; http://dx.doi.org/10.5353/th_b5351033 ; http://hdl.handle.net/10722/208041.

MLA Handbook (7^th Edition):

Chan, Yan-yan. “The role of cyclin E1 in hepatocellular carcinoma.” 2014. Web. 16 Sep 2019.

Vancouver:

Chan Y. The role of cyclin E1 in hepatocellular carcinoma. [Internet] [Masters thesis]. University of Hong Kong; 2014. [cited 2019 Sep 16]. Available from: Chan, Y. [陳茵茵]. (2014). The role of cyclin E1 in hepatocellular carcinoma. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5351033 ; http://dx.doi.org/10.5353/th_b5351033 ; http://hdl.handle.net/10722/208041.

Council of Science Editors:

Chan Y. The role of cyclin E1 in hepatocellular carcinoma. [Masters Thesis]. University of Hong Kong; 2014. Available from: Chan, Y. [陳茵茵]. (2014). The role of cyclin E1 in hepatocellular carcinoma. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5351033 ; http://dx.doi.org/10.5353/th_b5351033 ; http://hdl.handle.net/10722/208041

University of Hong Kong

6. Ng, Pik-yi. Molecular characterization of clostridium difficile PCR ribotypes R-002 and R-017 causing outbreaks and sporadic diarrhea.

Degree: Master of Medical Sciences, 2014, University of Hong Kong

URL: Ng, P. [吳碧儀]. (2014). Molecular characterization of clostridium difficile PCR ribotypes R-002 and R-017 causing outbreaks and sporadic diarrhea. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5361040 ; http://dx.doi.org/10.5353/th_b5361040 ; http://hdl.handle.net/10722/208523

►

Introduction: Clostridium difficile infection (CDI) is the major bacterial agent causing hospital-acquired diarrhea. The incidence and severity of CDI has increased significantly in recent decades.… (more)

▼

Introduction: Clostridium difficile infection (CDI) is the major bacterial agent causing hospital-acquired diarrhea. The incidence and severity of CDI has increased significantly in recent decades. The distribution of PCR ribotype varies among the countries. PCR ribotype 017 and 002 are the predominant ribotypes in China and Hong Kong respectively. Molecular characterization of C. difficile isolates is useful for outbreak investigation and surveillance. Both PCR ribotyping and Multilocus variable-number tandem-repeat analysis (MLVA) are reliable molecular tools for characterization of C. difficile from outbreak and sporadic case. MLVA allows subtyping of genetically related C. difficile isolates of the same ribotype by their distinctive MLVA patterns. The aim of this study was to characterize the outbreak and sporadic C. difficile isolates of PCR ribotype 002 and 017 by using capillary gel electrophoresis-based PCR ribotyping and MLVA. Their cytotoxicity, sporulation rate and germination efficiency were also investigated in the study. Results: A total of the 26 C. difficile isolates were identified by PCR ribotyping including fifteen isolates of PCR ribotype 002, eight isolates of ribotype 017, and three isolates of other ribotypes. The isolates of the same ribotypes were further sub-typed into outbreak and sporadic cluster. All isolates showed toxin-producing capability. The sporualtion rates of outbreak isolates of PCR ribotype 002 were significantly higher than that of sporadic isolates of PCR ribotype 002. Statistically higher sporulation rate was also observed in the outbreak isolates of PCR ribotype 017. The germination rate was also statistically higher in the outbreak isolates of PCR ribotype 002 than the sporadic cases. Conclusion: Capillary electrophoresis-based PCR ribotyping identified fifteen C. difficile PCR ribotype 002 and eight PCR ribotype 017 in this study. MLVA then refined these isolates into two corresponding sub-groups of outbreak and sporadic isolates. Higher sporulation and germination rates were observed in the outbreak isolates. Sporadic isolates demonstrated relatively lower sporulation and germination rates. The current evidences correlate the genotypic characterization to sporulation activity and germination efficiency.

published_or_final_version

Microbiology

Master

Master of Medical Sciences

Subjects/Keywords: Clostridium difficile - Molecular aspects

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APA (6^th Edition):

Ng, P. (2014). Molecular characterization of clostridium difficile PCR ribotypes R-002 and R-017 causing outbreaks and sporadic diarrhea. (Masters Thesis). University of Hong Kong. Retrieved from Ng, P. [吳碧儀]. (2014). Molecular characterization of clostridium difficile PCR ribotypes R-002 and R-017 causing outbreaks and sporadic diarrhea. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5361040 ; http://dx.doi.org/10.5353/th_b5361040 ; http://hdl.handle.net/10722/208523

Chicago Manual of Style (16^th Edition):

Ng, Pik-yi. “Molecular characterization of clostridium difficile PCR ribotypes R-002 and R-017 causing outbreaks and sporadic diarrhea.” 2014. Masters Thesis, University of Hong Kong. Accessed September 16, 2019. Ng, P. [吳碧儀]. (2014). Molecular characterization of clostridium difficile PCR ribotypes R-002 and R-017 causing outbreaks and sporadic diarrhea. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5361040 ; http://dx.doi.org/10.5353/th_b5361040 ; http://hdl.handle.net/10722/208523.

MLA Handbook (7^th Edition):

Ng, Pik-yi. “Molecular characterization of clostridium difficile PCR ribotypes R-002 and R-017 causing outbreaks and sporadic diarrhea.” 2014. Web. 16 Sep 2019.

Vancouver:

Ng P. Molecular characterization of clostridium difficile PCR ribotypes R-002 and R-017 causing outbreaks and sporadic diarrhea. [Internet] [Masters thesis]. University of Hong Kong; 2014. [cited 2019 Sep 16]. Available from: Ng, P. [吳碧儀]. (2014). Molecular characterization of clostridium difficile PCR ribotypes R-002 and R-017 causing outbreaks and sporadic diarrhea. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5361040 ; http://dx.doi.org/10.5353/th_b5361040 ; http://hdl.handle.net/10722/208523.

Council of Science Editors:

Ng P. Molecular characterization of clostridium difficile PCR ribotypes R-002 and R-017 causing outbreaks and sporadic diarrhea. [Masters Thesis]. University of Hong Kong; 2014. Available from: Ng, P. [吳碧儀]. (2014). Molecular characterization of clostridium difficile PCR ribotypes R-002 and R-017 causing outbreaks and sporadic diarrhea. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5361040 ; http://dx.doi.org/10.5353/th_b5361040 ; http://hdl.handle.net/10722/208523

University of Hong Kong

7. 賈亦真; Jia, Yizhen. Integrated bioinformatics analysis of genetic, expression and interaction data to understand the molecular mechanisms of Alzheimer's disease.

Degree: PhD, 2014, University of Hong Kong

URL: Jia, Y. [賈亦真]. (2014). Integrated bioinformatics analysis of genetic, expression and interaction data to understand the molecular mechanisms of Alzheimer's disease. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5570785 ; http://hdl.handle.net/10722/219981

► This thesis presents an integrated analysis to understand different aspects of the molecular mechanisms of Alzheimer’s disease (AD). Genetics data from genome-wide association studies (GWASs),… (more)

▼ This thesis presents an integrated analysis to understand different aspects of the molecular mechanisms of Alzheimer’s disease (AD). Genetics data from genome-wide association studies (GWASs), expression data from newly developed RNA-seq technology and published microarray experiments, and interaction data from public protein database were integrated to build up networks using bioinformatics and systems biology approaches. Previous experiments have identified Pax6, a multifunctional transcription factor which plays essential roles in eye development and pancreatic β-cell function, as a new regulator involved in the cell-cycle related neuronal death model of AD. However, downstream targets of Pax6 in brain remains undiscovered and upon up-regulation of Pax6, the downstream molecular mechanisms by which neuronal death is triggered and eventually leads to AD are largely unclear. In Chapter 3, downstream network of Pax6 in AD neuronal death model was constructed by integrating expression data of AD brain, Pax6 binding sites prediction and literature search. Expression level of Pax6 significantly increased during AD progression among different brain regions, shown by microarray. 70 potential downstream targets of Pax6, including ACAD8, YWHAZ and OGT, were identified and ranked by their binding affinity. Network was constructed based on these 70 candidates. 9 KEGG pathways harboring well-defined AD-related sub-pathways were identified, among which the PI3K/PDK1/AKT sub-pathway was shown to be activated in AD brain. In Chapter 4, expression alterations after Pax6 knockdown were examined by siPax6 in mouse cortical neurons followed by RNA-seq. An analysis pipeline was built to quantify expression level, identify splicing isoforms and discover new genes/transcripts from raw RNA-seq data. 8,584 genes were identified as differentially expressed (DEGs) after Pax6 knockdown, validated preliminarily by qPCR random sampling of 10 DEGs. KEGG pathways interrupted by Pax6 knockdown were largely overlapped with AD-related pathways identified in Chapter 3. GSK3B was selected as prime candidate downstream of Pax6. Cell cycle/Pax6/GSK3β pathway provides a possible link from Aβ-mediated neuronal death to tau pathology. Chapter 3&4 both indicated the role of Pax6/GSK3β-mediated insulin signaling pathway in AD. Type 2 diabetes mellitus (T2DM), characterized by insulin resistance and interrupted insulin signaling, is a strong risk factor of AD. An integrated analysis was carried out to study the shared molecular mechanisms linking AD and T2DM. APOE, APOC1 and TOMM40 were identified as common GWAS genes. 40 pathways were enriched in both diseases, including PI3K/AKT mediated insulin signaling pathways. Stronger connections between AD and T2DM GWAS genes were detected compared with random generated genes and bone mineral density (BMD) GWAS genes. TNF, MMP3, PPARG and CLU were identified as hub-/bottleneck-genes in the connection network which might perform the most essential functions in the progression of AD for patients with…

Subjects/Keywords: Alzheimer's disease - Molecular aspects; Bioinformatics

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APA (6^th Edition):

賈亦真; Jia, Y. (2014). Integrated bioinformatics analysis of genetic, expression and interaction data to understand the molecular mechanisms of Alzheimer's disease. (Doctoral Dissertation). University of Hong Kong. Retrieved from Jia, Y. [賈亦真]. (2014). Integrated bioinformatics analysis of genetic, expression and interaction data to understand the molecular mechanisms of Alzheimer's disease. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5570785 ; http://hdl.handle.net/10722/219981

Chicago Manual of Style (16^th Edition):

賈亦真; Jia, Yizhen. “Integrated bioinformatics analysis of genetic, expression and interaction data to understand the molecular mechanisms of Alzheimer's disease.” 2014. Doctoral Dissertation, University of Hong Kong. Accessed September 16, 2019. Jia, Y. [賈亦真]. (2014). Integrated bioinformatics analysis of genetic, expression and interaction data to understand the molecular mechanisms of Alzheimer's disease. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5570785 ; http://hdl.handle.net/10722/219981.

MLA Handbook (7^th Edition):

賈亦真; Jia, Yizhen. “Integrated bioinformatics analysis of genetic, expression and interaction data to understand the molecular mechanisms of Alzheimer's disease.” 2014. Web. 16 Sep 2019.

Vancouver:

賈亦真; Jia Y. Integrated bioinformatics analysis of genetic, expression and interaction data to understand the molecular mechanisms of Alzheimer's disease. [Internet] [Doctoral dissertation]. University of Hong Kong; 2014. [cited 2019 Sep 16]. Available from: Jia, Y. [賈亦真]. (2014). Integrated bioinformatics analysis of genetic, expression and interaction data to understand the molecular mechanisms of Alzheimer's disease. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5570785 ; http://hdl.handle.net/10722/219981.

Council of Science Editors:

賈亦真; Jia Y. Integrated bioinformatics analysis of genetic, expression and interaction data to understand the molecular mechanisms of Alzheimer's disease. [Doctoral Dissertation]. University of Hong Kong; 2014. Available from: Jia, Y. [賈亦真]. (2014). Integrated bioinformatics analysis of genetic, expression and interaction data to understand the molecular mechanisms of Alzheimer's disease. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5570785 ; http://hdl.handle.net/10722/219981

University of Hong Kong

8. Liu, Yang. The role of regulatory B cells in the development of autoimmune diabetes in NOD mice.

Degree: M. Phil., 2013, University of Hong Kong

URL: Liu, Y. [劉洋]. (2013). The role of regulatory B cells in the development of autoimmune diabetes in NOD mice. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5194749 ; http://dx.doi.org/10.5353/th_b5194749 ; http://hdl.handle.net/10722/197509

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Interleukin (IL)-10-secreting regulatory Bcells(B10) are acknowledged to play important roles in balancing cellular immunity and fighting against autoimmune diseases. Since the early discovery of the… (more)

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Interleukin (IL)-10-secreting regulatory Bcells(B10) are acknowledged to play important roles in balancing cellular immunity and fighting against autoimmune diseases. Since the early discovery of the potential of B cells in suppressing autoimmunity by secreting IL-10 in a murine model of experimental autoimmune encephalomyelitis(EAE),accumulating evidences have revealed the existence and regulatory function of B10 cells in the progression of several autoimmune diseases including multiple sclerosis (MS), lupus and autoimmune arthritis, suggesting potential values of therapeutic intervention. Autoimmune diabetes is an autoimmune disease in animal models characterized by progressive insulitis and mass destruction of βcells in pancreatic islets. However, the role of Bregsin the development of this disease remains largely unclear. To explore whether Bregs possess a regulatory function in suppressing diabetes, B10 cells were generated from B-cell activation factor (BAFF)-stimulated B cells of Non-obese diabetic (NOD)mice. Notably, NOD mice receiving B10 transfer exhibited delayed diabetes onset and substantially reduced incidence, suggesting some therapeutic effect against autoimmune diabetes. As an important contributor to inflammation and autoimmune disorders, the pathogenic function of IL-17 producing CD4+cells (Th17) in autoimmune diabetes has been increasingly identified, which attracts me to investigate whether B10 cells can contribute to amelioration of autoimmune diabetes via suppressing Th17 cells. During the development of autoimmune diabetes in NOD mice, both B10 and Th17 significantly increased at prediabetic stage and rapidly declined after disease onset. Upon adoptive transfer of B10 cells into prediabetic NOD mice, Th17 cells in pancreatic lymph nodes and pancreas were profoundly reduced. To verify whether B10 cells can directly inhibit Th17 generation in vitro, CFSE-dilution assay combined to Th17 polarization assay was performed. Results indicated that B10 cells suppress Th17 polarization in an IL-10 independent manner, but inhibit Th17 proliferation in an partially IL-10 dependent way. Finally I transferred B10 together with naive CD4+T cells reactive to islets into lymphopenic NOD-SCID mice and detected substantially reduced Th17 frequencies in pancreatic lymph nodes and pancreas, suggesting a potential way of developing new therapeutic strategies in treating Type 1 diabetes in humans.

published_or_final_version

Pathology

Master

Master of Philosophy

Advisors/Committee Members: Lu, L.

Subjects/Keywords: Diabetes - Molecular aspects; B cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Liu, Y. (2013). The role of regulatory B cells in the development of autoimmune diabetes in NOD mice. (Masters Thesis). University of Hong Kong. Retrieved from Liu, Y. [劉洋]. (2013). The role of regulatory B cells in the development of autoimmune diabetes in NOD mice. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5194749 ; http://dx.doi.org/10.5353/th_b5194749 ; http://hdl.handle.net/10722/197509

Chicago Manual of Style (16^th Edition):

Liu, Yang. “The role of regulatory B cells in the development of autoimmune diabetes in NOD mice.” 2013. Masters Thesis, University of Hong Kong. Accessed September 16, 2019. Liu, Y. [劉洋]. (2013). The role of regulatory B cells in the development of autoimmune diabetes in NOD mice. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5194749 ; http://dx.doi.org/10.5353/th_b5194749 ; http://hdl.handle.net/10722/197509.

MLA Handbook (7^th Edition):

Liu, Yang. “The role of regulatory B cells in the development of autoimmune diabetes in NOD mice.” 2013. Web. 16 Sep 2019.

Vancouver:

Liu Y. The role of regulatory B cells in the development of autoimmune diabetes in NOD mice. [Internet] [Masters thesis]. University of Hong Kong; 2013. [cited 2019 Sep 16]. Available from: Liu, Y. [劉洋]. (2013). The role of regulatory B cells in the development of autoimmune diabetes in NOD mice. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5194749 ; http://dx.doi.org/10.5353/th_b5194749 ; http://hdl.handle.net/10722/197509.

Council of Science Editors:

Liu Y. The role of regulatory B cells in the development of autoimmune diabetes in NOD mice. [Masters Thesis]. University of Hong Kong; 2013. Available from: Liu, Y. [劉洋]. (2013). The role of regulatory B cells in the development of autoimmune diabetes in NOD mice. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5194749 ; http://dx.doi.org/10.5353/th_b5194749 ; http://hdl.handle.net/10722/197509

University of Hong Kong

9. Wang, Yongfeng. Molecular analysis of ammonia oxidizing prokaryotes in mangrove wetlands and factors affecting their dynamics.

Degree: PhD, 2013, University of Hong Kong

URL: Wang, Y. [王永峰]. (2013). Molecular analysis of ammonia oxidizing prokaryotes in mangrove wetlands and factors affecting their dynamics. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5043442 ; http://dx.doi.org/10.5353/th_b5043442 ; http://hdl.handle.net/10722/184255

► Ammonia/ammonium-oxidizing prokaryotes (AOPs) play a crucial role in nitrogen transformation in the natural ecosystems including mangrove wetlands. The objectives of the present study were to… (more)

▼ Ammonia/ammonium-oxidizing prokaryotes (AOPs) play a crucial role in nitrogen transformation in the natural ecosystems including mangrove wetlands. The objectives of the present study were to investigate the spatial and temporal distribution of AOPs in the mangrove wetland sediments in subtropical Hong Kong and their ecophysiology. When AOP communities and abundances in a natural coastal mangrove wetland and a constructed freshwater wetland were compared, the constructed freshwater wetland contained a broader range of phylotypes, higher diversity, more complex community structures, and more uneven abundances of AOPs than the mangrove wetland. Typha angustifolia affected the community structures of all AOPs and enhanced their abundances in the rhizosphere. Both Phragmites australis and Cyperus malaccensis showed some effects on the community structures of ammonia-oxidizing bacteria (AOB), but little effects on those of anaerobic ammonium oxidizing (anammox) bacteria or ammonia-oxidizing archaea (AOA). Kandelia obovata had no detectable effect on any group of the AOPs due to their smaller size. AOPs in oxic and anoxic sediments of a protected mangrove wetland were investigated in both winter and summer. Seasonality had little effect on community structure and abundance of anammox bacteria. AOA community structures were stable between the two seasons, but AOA abundance was significantly higher in winter than summer. The community structures of AOB were different between winter and summer, but the abundance in winter was apparently higher than that in summer. Sediment type had a noticeable influence on community structure and abundance of anammox bacteria. No apparent difference in AOA community structures between the different types of sediments in winter was observed, but the oxic sediments showed obviously different AOA community structures from anoxic sediments in summer. Sediment type had little effect on AOB community structures, but AOB abundance in oxic sediments was obviously lower than anoxic ones in both seasons. Addition of acetate or leaf litter into sediment inhibited the growth of anammox bacteria in laboratory incubation. The inhibition of anammox bacteria by acetate was more pronounced than by leaves. Acetate and leaf litter did not affect AOA community structures, but promoted their growth. Both acetate and leaf litter affected the AOB community structures and promoted their growth in the early phase of the incubation. The promoting effects by leaf litter were more obvious than by acetate. Allylthiourea effectively inhibited the growth of both AOA and AOB in laboratory incubation, but only slightly for anammox bacteria. Acidic condition altered AOB community structure, but affected anammox bacteria and AOA slightly. Alkaline condition strongly affected community structures of anammox bacteria and AOA, but slightly for AOB. Alkaline condition inhibited the growth of anammox bacteria, but promoted AOA and AOB slightly. Increase in salinity resulted in higher diversity of anammox bacteria, and… Advisors/Committee Members: Gu, J.

Subjects/Keywords: Prokaryotes - Molecular aspects.; Mangrove ecology.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wang, Y. (2013). Molecular analysis of ammonia oxidizing prokaryotes in mangrove wetlands and factors affecting their dynamics. (Doctoral Dissertation). University of Hong Kong. Retrieved from Wang, Y. [王永峰]. (2013). Molecular analysis of ammonia oxidizing prokaryotes in mangrove wetlands and factors affecting their dynamics. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5043442 ; http://dx.doi.org/10.5353/th_b5043442 ; http://hdl.handle.net/10722/184255

Chicago Manual of Style (16^th Edition):

Wang, Yongfeng. “Molecular analysis of ammonia oxidizing prokaryotes in mangrove wetlands and factors affecting their dynamics.” 2013. Doctoral Dissertation, University of Hong Kong. Accessed September 16, 2019. Wang, Y. [王永峰]. (2013). Molecular analysis of ammonia oxidizing prokaryotes in mangrove wetlands and factors affecting their dynamics. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5043442 ; http://dx.doi.org/10.5353/th_b5043442 ; http://hdl.handle.net/10722/184255.

MLA Handbook (7^th Edition):

Wang, Yongfeng. “Molecular analysis of ammonia oxidizing prokaryotes in mangrove wetlands and factors affecting their dynamics.” 2013. Web. 16 Sep 2019.

Vancouver:

Wang Y. Molecular analysis of ammonia oxidizing prokaryotes in mangrove wetlands and factors affecting their dynamics. [Internet] [Doctoral dissertation]. University of Hong Kong; 2013. [cited 2019 Sep 16]. Available from: Wang, Y. [王永峰]. (2013). Molecular analysis of ammonia oxidizing prokaryotes in mangrove wetlands and factors affecting their dynamics. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5043442 ; http://dx.doi.org/10.5353/th_b5043442 ; http://hdl.handle.net/10722/184255.

Council of Science Editors:

Wang Y. Molecular analysis of ammonia oxidizing prokaryotes in mangrove wetlands and factors affecting their dynamics. [Doctoral Dissertation]. University of Hong Kong; 2013. Available from: Wang, Y. [王永峰]. (2013). Molecular analysis of ammonia oxidizing prokaryotes in mangrove wetlands and factors affecting their dynamics. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5043442 ; http://dx.doi.org/10.5353/th_b5043442 ; http://hdl.handle.net/10722/184255

University of Hong Kong

10. 江家耀; Kong, Ka-yiu. Characterization of the roles of yeast nuclear exosome cofactor TRAMP complex in pre-mRNA splicing.

Degree: PhD, 2013, University of Hong Kong

URL: Kong, K. [江家耀]. (2013). Characterization of the roles of yeast nuclear exosome cofactor TRAMP complex in pre-mRNA splicing. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5108671 ; http://dx.doi.org/10.5353/th_b5108671 ; http://hdl.handle.net/10722/193522

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In budding yeast, the Trf4/5p-Air1/2p-Mtr4p polyadenylation (TRAMP) complex recognizes unwanted RNA transcripts in the nucleus and then targets them to the nuclear exosome for rapid… (more)

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In budding yeast, the Trf4/5p-Air1/2p-Mtr4p polyadenylation (TRAMP) complex recognizes unwanted RNA transcripts in the nucleus and then targets them to the nuclear exosome for rapid degradation, constituting an important pathway of nuclear RNA quality control. Each pre-mRNA splicing event unavoidably generates a RNA side-product that should be recognized by TRAMP and then removed by the nuclear exosome to prevent the potentially harmful sequestration of splicing factors and/or ribonucleotides. While successful pre-mRNA splicing inevitably produces a spliced-out intron, errors in pre-mRNA splicing lead to the emergence of either an abnormal splicing intermediate, or a splicing-incompetent pre-mRNA that cannot be properly spliced. However, it remains unclear how and when these RNA side-products of pre-mRNA splicing are recognized by TRAMP. In this study, chromatin immunoprecipitation (ChIP) was applied to demonstrate that both TRAMP and the nuclear exosome component Rrp6p are cotranscriptionally recruited to nascent RNA transcripts, particularly to intronic sequences, indicating that splicing side-products are recognized by TRAMP and committed to subsequent nuclear-exosome-mediated degradation in a cotranscriptional manner. Deletion of TRF4, of both AIR1 and AIR2, or of RRP6, resulted in accumulation of unspliced pre-mRNAs. Surprisingly, while such pre-mRNAs accumulated in rrp6 cells owing to defects in pre-mRNA degradation, the same phenotype in trf4 and air1air2 cells involved splicing defects, demonstrating that only TRAMP, but not the nuclear exosome, contributes to optimal pre-mRNA splicing. Consistent with a direct stimulatory role for TRAMP in pre-mRNA splicing, negative genetic interactions and physical interactions between Trf4p and several splicing factors were observed, and that Trf4p was further shown to be required for optimal recruitment of the splicing factor Msl5p. The direct facilitation of pre-mRNA splicing by TRAMP may act as a fail-safe mechanism to ensure the cotranscriptional recruitment of TRAMP to nascent intron-containing transcripts before or during pre-mRNA splicing, such that the subsequently generated spliced-out introns, abnormal splicing intermediates, or splicing-incompetent pre-mRNAs can be recognized immediately by TRAMP, and then targeted to the nuclear exosome for prompt degradation before their potentially harmful accumulation. Since most TRAMP and nuclear exosome components found in budding yeast also contain functional human homologs, this work provides important insights into how splicing side-products are rapidly degraded by the nuclear RNA quality control system in human cells, which have a much higher frequency of introns within their genome, and certainly require a much more efficient pathway for the removal of an increased amount of splicing side-products due to the greater number of splicing events.

published_or_final_version

Biochemistry

Doctoral

Doctor of Philosophy

Advisors/Committee Members: Jin, D, Yao, KM.

Subjects/Keywords: Messenger RNA; Yeast - Molecular aspects

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

江家耀; Kong, K. (2013). Characterization of the roles of yeast nuclear exosome cofactor TRAMP complex in pre-mRNA splicing. (Doctoral Dissertation). University of Hong Kong. Retrieved from Kong, K. [江家耀]. (2013). Characterization of the roles of yeast nuclear exosome cofactor TRAMP complex in pre-mRNA splicing. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5108671 ; http://dx.doi.org/10.5353/th_b5108671 ; http://hdl.handle.net/10722/193522

Chicago Manual of Style (16^th Edition):

江家耀; Kong, Ka-yiu. “Characterization of the roles of yeast nuclear exosome cofactor TRAMP complex in pre-mRNA splicing.” 2013. Doctoral Dissertation, University of Hong Kong. Accessed September 16, 2019. Kong, K. [江家耀]. (2013). Characterization of the roles of yeast nuclear exosome cofactor TRAMP complex in pre-mRNA splicing. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5108671 ; http://dx.doi.org/10.5353/th_b5108671 ; http://hdl.handle.net/10722/193522.

MLA Handbook (7^th Edition):

江家耀; Kong, Ka-yiu. “Characterization of the roles of yeast nuclear exosome cofactor TRAMP complex in pre-mRNA splicing.” 2013. Web. 16 Sep 2019.

Vancouver:

江家耀; Kong K. Characterization of the roles of yeast nuclear exosome cofactor TRAMP complex in pre-mRNA splicing. [Internet] [Doctoral dissertation]. University of Hong Kong; 2013. [cited 2019 Sep 16]. Available from: Kong, K. [江家耀]. (2013). Characterization of the roles of yeast nuclear exosome cofactor TRAMP complex in pre-mRNA splicing. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5108671 ; http://dx.doi.org/10.5353/th_b5108671 ; http://hdl.handle.net/10722/193522.

Council of Science Editors:

江家耀; Kong K. Characterization of the roles of yeast nuclear exosome cofactor TRAMP complex in pre-mRNA splicing. [Doctoral Dissertation]. University of Hong Kong; 2013. Available from: Kong, K. [江家耀]. (2013). Characterization of the roles of yeast nuclear exosome cofactor TRAMP complex in pre-mRNA splicing. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5108671 ; http://dx.doi.org/10.5353/th_b5108671 ; http://hdl.handle.net/10722/193522

University of Hong Kong

11. Lam, Ching-to. Molecular characterization of clostridium difficile isolates by capillary gel electrophoresis-based PCR ribotyping.

Degree: Master of Medical Sciences, 2012, University of Hong Kong

URL: Lam, C. [林正道]. (2012). Molecular characterization of clostridium difficile isolates by capillary gel electrophoresis-based PCR ribotyping. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4833399 ; http://dx.doi.org/10.5353/th_b4833399 ; http://hdl.handle.net/10722/173847

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Backgroud: Clostridium difficile infection (CDI) is a global concern since the emergence of the hyper-virulent strain NAP1/BI/027. The incidence of CDI has been increasing and… (more)

Subjects/Keywords: Clostridium difficile - Molecular aspects.

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APA (6^th Edition):

Lam, C. (2012). Molecular characterization of clostridium difficile isolates by capillary gel electrophoresis-based PCR ribotyping. (Masters Thesis). University of Hong Kong. Retrieved from Lam, C. [林正道]. (2012). Molecular characterization of clostridium difficile isolates by capillary gel electrophoresis-based PCR ribotyping. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4833399 ; http://dx.doi.org/10.5353/th_b4833399 ; http://hdl.handle.net/10722/173847

Chicago Manual of Style (16^th Edition):

Lam, Ching-to. “Molecular characterization of clostridium difficile isolates by capillary gel electrophoresis-based PCR ribotyping.” 2012. Masters Thesis, University of Hong Kong. Accessed September 16, 2019. Lam, C. [林正道]. (2012). Molecular characterization of clostridium difficile isolates by capillary gel electrophoresis-based PCR ribotyping. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4833399 ; http://dx.doi.org/10.5353/th_b4833399 ; http://hdl.handle.net/10722/173847.

MLA Handbook (7^th Edition):

Lam, Ching-to. “Molecular characterization of clostridium difficile isolates by capillary gel electrophoresis-based PCR ribotyping.” 2012. Web. 16 Sep 2019.

Vancouver:

Lam C. Molecular characterization of clostridium difficile isolates by capillary gel electrophoresis-based PCR ribotyping. [Internet] [Masters thesis]. University of Hong Kong; 2012. [cited 2019 Sep 16]. Available from: Lam, C. [林正道]. (2012). Molecular characterization of clostridium difficile isolates by capillary gel electrophoresis-based PCR ribotyping. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4833399 ; http://dx.doi.org/10.5353/th_b4833399 ; http://hdl.handle.net/10722/173847.

Council of Science Editors:

Lam C. Molecular characterization of clostridium difficile isolates by capillary gel electrophoresis-based PCR ribotyping. [Masters Thesis]. University of Hong Kong; 2012. Available from: Lam, C. [林正道]. (2012). Molecular characterization of clostridium difficile isolates by capillary gel electrophoresis-based PCR ribotyping. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4833399 ; http://dx.doi.org/10.5353/th_b4833399 ; http://hdl.handle.net/10722/173847

Massey University

12. Rehman, Zahid ur. Molecular mechanism of export of alginate in Pseudomonas aeruginosa : a thesis presented in partial fulfilment of the requirements for [the] degree of Doctor of Philosophy in Microbiology at Massey University, New Zealand .

Degree: 2013, Massey University

URL: http://hdl.handle.net/10179/4322

► Pseudomonas aeruginosa is an opportunistic pathogen; infecting insects, plants and humans. It is of particular relevance to cystic fibrosis (CF) patients where it causes pulmonary… (more)

Subjects/Keywords: Pseudomonas aeruginosa; Molecular aspects; Alginates

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APA (6^th Edition):

Rehman, Z. u. (2013). Molecular mechanism of export of alginate in Pseudomonas aeruginosa : a thesis presented in partial fulfilment of the requirements for [the] degree of Doctor of Philosophy in Microbiology at Massey University, New Zealand . (Thesis). Massey University. Retrieved from http://hdl.handle.net/10179/4322

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rehman, Zahid ur. “Molecular mechanism of export of alginate in Pseudomonas aeruginosa : a thesis presented in partial fulfilment of the requirements for [the] degree of Doctor of Philosophy in Microbiology at Massey University, New Zealand .” 2013. Thesis, Massey University. Accessed September 16, 2019. http://hdl.handle.net/10179/4322.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rehman, Zahid ur. “Molecular mechanism of export of alginate in Pseudomonas aeruginosa : a thesis presented in partial fulfilment of the requirements for [the] degree of Doctor of Philosophy in Microbiology at Massey University, New Zealand .” 2013. Web. 16 Sep 2019.

Vancouver:

Rehman Zu. Molecular mechanism of export of alginate in Pseudomonas aeruginosa : a thesis presented in partial fulfilment of the requirements for [the] degree of Doctor of Philosophy in Microbiology at Massey University, New Zealand . [Internet] [Thesis]. Massey University; 2013. [cited 2019 Sep 16]. Available from: http://hdl.handle.net/10179/4322.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rehman Zu. Molecular mechanism of export of alginate in Pseudomonas aeruginosa : a thesis presented in partial fulfilment of the requirements for [the] degree of Doctor of Philosophy in Microbiology at Massey University, New Zealand . [Thesis]. Massey University; 2013. Available from: http://hdl.handle.net/10179/4322

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University

13. Njuguna, Wambui. Development and use of molecular tools in Fragaria.

Degree: PhD, Horticulture, 2010, Oregon State University

URL: http://hdl.handle.net/1957/15096

► This dissertation describes the application and development of molecular tools that have great potential for use in studying variation in strawberry germplasm. The first study… (more)

▼ This dissertation describes the application and development of molecular tools that have great potential for use in studying variation in strawberry germplasm. The first study evaluated 91 microsatellite (simple sequence repeat, SSR) markers for transferability in 22 Fragaria species and their utility in fingerprinting octoploid strawberries. Out of the transferable markers, a reduced set of four SSRs was developed based on polymorphism, multiplexing ability, reproducibility and ease of scoring. Unique SSR fingerprints were obtained for over 175 Fragaria samples representing 22 Fragaria species used in the study. Testing of two molecular markers linked to the red stele and anthracnose resistances identified potential sources of resistance in previously untested genotypes. Further characterization of these accessions is warranted to validate resistance and usefulness in breeding. In the second study, 20 SSRs polymorphic in wild Asian diploids, F. iinumae and F. nipponica, from Hokkaido, Japan were selected for genetic analysis of 137 accessions from 22 locations. Principal coordinate analysis followed by non-parametric modal clustering grouped accessions into two groups representing the two species. Further clustering within the groups resulted in 10 groups (7-F. iinumae, 3-F. nipponica) that suggest lineages representative of the diversity in Hokkaido, Japan. The third study tested plant DNA barcodes, the nuclear ribosomal Internal Transcribed and the chloroplast PsbA-trnH spacers, for Fragaria species identification. The 'barcoding gap', between within species and between species variation, was absent and prevented identification of Fragaria species. The fourth study evaluated the genetic diversity of 94 accessions representing 22 Fragaria species using four universal chloroplast SSR loci. Genetic diversity was moderate (0.54) despite the homoplasy observed. Species-specific haplotypes for F. nipponica, F. orientalis, F. iinumae and F. nilgerrensis were identified. Sequencing whole chloroplast genomes using Illumina in a final study revealed a close maternal genome relationship between F. vesca ssp. bracteata and the octoploid species supporting a North American origin of the octoploids and the polyphyly of F. vesca. Calculation of divergence time of Fragaria revealed young evolutionary age of the genus at 2.7 million years and of the octoploids at 450,000 years. Advisors/Committee Members: Bassil, Nahla V. (advisor), Mehlenbacher, Shawn (committee member).

Subjects/Keywords: strawberries; Strawberries – Molecular aspects

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Njuguna, W. (2010). Development and use of molecular tools in Fragaria. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/15096

Chicago Manual of Style (16^th Edition):

Njuguna, Wambui. “Development and use of molecular tools in Fragaria.” 2010. Doctoral Dissertation, Oregon State University. Accessed September 16, 2019. http://hdl.handle.net/1957/15096.

MLA Handbook (7^th Edition):

Njuguna, Wambui. “Development and use of molecular tools in Fragaria.” 2010. Web. 16 Sep 2019.

Vancouver:

Njuguna W. Development and use of molecular tools in Fragaria. [Internet] [Doctoral dissertation]. Oregon State University; 2010. [cited 2019 Sep 16]. Available from: http://hdl.handle.net/1957/15096.

Council of Science Editors:

Njuguna W. Development and use of molecular tools in Fragaria. [Doctoral Dissertation]. Oregon State University; 2010. Available from: http://hdl.handle.net/1957/15096

Oregon State University

14. Kepler, Ryan M. Advances in molecular systematics of clavicipitaceous fungi (Sordariomycetes: Hypocreales).

Degree: PhD, Botany and Plant Pathology, 2010, Oregon State University

URL: http://hdl.handle.net/1957/19589

► Historical concepts of Clavicipitaceae have included a broad range of species that display diverse morphologies, ecological modes and host associations. When subjected to multigene phylogenetic… (more)

▼ Historical concepts of Clavicipitaceae have included a broad range of species that display diverse morphologies, ecological modes and host associations. When subjected to multigene phylogenetic investigation of evolutionary history, the family was found to be polyphyletic, largely driven by diversity in the genus Cordyceps, previously containing over 400 taxa. The majority of Cordyceps sensu lato now resides in the families Cordycipitaceae, for which the genus Cordyceps has been retained owing to the placement of the type C. militaris, and the genera Ophiocordyceps and Elaphocordyceps in Ophiocordycipitaceae. The genus Metacordyceps was defined for species of Cordyceps remaining in Clavicipitaceae sensu stricto and contains relatively few species owing to convergent morphologies. Clavicipitaceae remains considerably diverse and its members attack hosts across three kingdoms of life, including insects and rotifers, plants and other fungi. There remains a significant number of Cordyceps species for which molecular or morphological data are insufficient, and are therefore considered incertae sedis with regards to family until new material is available for examination. This work expands the sampling of taxa in Clavicipitaceae sensu lato for inclusion in phylogenetic reconstruction, with particular emphasis on residual species of Cordyceps. The resulting phylogenies were then used to refine concepts morphological features that define boundaries between taxa and explore the evolution of host association, morphology and ecology with ancestral character-state reconstruction. Taxon sampling was increased by extensive field collections and collaborations with other researchers. The diversity present in newly acquired specimens represents all three pathogen groups. The inclusion of these taxa in a five-gene dataset (nrSSU, nrLSU, TEF, RPB1, RPB2) advances understanding of systematic relationships within Clavicipitaceae. Molecular data supports the movement of seven species from Cordyceps sensu lato to Metacordyceps, doubling the number of species known for the genus. The addition of new species reveals significant structure within the genus and allows for a reevaluation and strengthening of morphological concepts attributed to the genus. A clade of species sister to Clavicipitaceae was identified as Veterocordyceps gen. nov. This finding provides clarity to the results of previous workers who considered species in the genus as members of Ophiocordyceps based on morphological data and a more limited molecular dataset. Cordyceps fratricida, a pathogen of ergot, and the rice pathogen Ustilaginoidea virens were placed phylogenetically within Clavicipitaceae. The genus Tyrranocordyceps gen. nov. was established for fungal pathogens that attack closely related species of ergot. Tyrranocordyceps represents a sexual state for a clade of fungal pathogens known only from asexual states. Ustilaginoidea virens was found to occupy a separate lineage of plant pathogens, representing a third clade of clavicipitaceous fungi deriving their… Advisors/Committee Members: Spatafora, Joseph W. (advisor), Muir, Patricia (committee member).

Subjects/Keywords: Fungi; Hypocreales – Phylogeny – Molecular aspects

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APA (6^th Edition):

Kepler, R. M. (2010). Advances in molecular systematics of clavicipitaceous fungi (Sordariomycetes: Hypocreales). (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/19589

Chicago Manual of Style (16^th Edition):

Kepler, Ryan M. “Advances in molecular systematics of clavicipitaceous fungi (Sordariomycetes: Hypocreales).” 2010. Doctoral Dissertation, Oregon State University. Accessed September 16, 2019. http://hdl.handle.net/1957/19589.

MLA Handbook (7^th Edition):

Kepler, Ryan M. “Advances in molecular systematics of clavicipitaceous fungi (Sordariomycetes: Hypocreales).” 2010. Web. 16 Sep 2019.

Vancouver:

Kepler RM. Advances in molecular systematics of clavicipitaceous fungi (Sordariomycetes: Hypocreales). [Internet] [Doctoral dissertation]. Oregon State University; 2010. [cited 2019 Sep 16]. Available from: http://hdl.handle.net/1957/19589.

Council of Science Editors:

Kepler RM. Advances in molecular systematics of clavicipitaceous fungi (Sordariomycetes: Hypocreales). [Doctoral Dissertation]. Oregon State University; 2010. Available from: http://hdl.handle.net/1957/19589

East Carolina University

15. Williams, Melinda. Analysis of the hex regulon in Pseudomonas aeruginosa.

Degree: 2012, East Carolina University

URL: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=13782

► Pseudomonas aeruginosa is an opportunistic pathogen whose defense mechanisms include biofilm production through carbohydrate metabolism. The activity of the enzymes in the hex regulon are… (more)

Subjects/Keywords: Pseudomonas aeruginosa – Molecular aspects

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APA (6^th Edition):

Williams, M. (2012). Analysis of the hex regulon in Pseudomonas aeruginosa. (Masters Thesis). East Carolina University. Retrieved from http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=13782

Chicago Manual of Style (16^th Edition):

Williams, Melinda. “Analysis of the hex regulon in Pseudomonas aeruginosa.” 2012. Masters Thesis, East Carolina University. Accessed September 16, 2019. http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=13782.

MLA Handbook (7^th Edition):

Williams, Melinda. “Analysis of the hex regulon in Pseudomonas aeruginosa.” 2012. Web. 16 Sep 2019.

Vancouver:

Williams M. Analysis of the hex regulon in Pseudomonas aeruginosa. [Internet] [Masters thesis]. East Carolina University; 2012. [cited 2019 Sep 16]. Available from: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=13782.

Council of Science Editors:

Williams M. Analysis of the hex regulon in Pseudomonas aeruginosa. [Masters Thesis]. East Carolina University; 2012. Available from: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=13782

University of North Carolina – Greensboro

16. Stacy, Anderson Timothy IV. Efforts towards the formal synthesis of bakuchiol via small molecule manipulation.

Degree: 2014, University of North Carolina – Greensboro

URL: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=16616

► Bakuchiol is a monoterpinoid compound found mainly in the seeds of Psoralea coryfolia Linn, which is indigenous to Southeast Asia. Since its discovery and isolation… (more)

Subjects/Keywords: Psoralea – Molecular aspects; Biosynthesis

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APA (6^th Edition):

Stacy, A. T. I. (2014). Efforts towards the formal synthesis of bakuchiol via small molecule manipulation. (Masters Thesis). University of North Carolina – Greensboro. Retrieved from http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=16616

Chicago Manual of Style (16^th Edition):

Stacy, Anderson Timothy IV. “Efforts towards the formal synthesis of bakuchiol via small molecule manipulation.” 2014. Masters Thesis, University of North Carolina – Greensboro. Accessed September 16, 2019. http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=16616.

MLA Handbook (7^th Edition):

Stacy, Anderson Timothy IV. “Efforts towards the formal synthesis of bakuchiol via small molecule manipulation.” 2014. Web. 16 Sep 2019.

Vancouver:

Stacy ATI. Efforts towards the formal synthesis of bakuchiol via small molecule manipulation. [Internet] [Masters thesis]. University of North Carolina – Greensboro; 2014. [cited 2019 Sep 16]. Available from: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=16616.

Council of Science Editors:

Stacy ATI. Efforts towards the formal synthesis of bakuchiol via small molecule manipulation. [Masters Thesis]. University of North Carolina – Greensboro; 2014. Available from: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=16616

Brock University

17. Zhang, Di. The structure of arabidopsis NPR1 : its function as a salicylic acid receptor and a metal-binding protein .

Degree: Centre for Biotechnology, 2011, Brock University

URL: http://hdl.handle.net/10464/3422

► The Arabidopsis NPRI protein regulates systemic acquired resistance dependent on salicylic acid. Analyses by plant two-hybrid analysis in vivo and pull-down assays in vitro showed… (more)

Subjects/Keywords: Arabidopsis – Molecular aspects.; Metalloproteins

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APA (6^th Edition):

Zhang, D. (2011). The structure of arabidopsis NPR1 : its function as a salicylic acid receptor and a metal-binding protein . (Thesis). Brock University. Retrieved from http://hdl.handle.net/10464/3422

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zhang, Di. “The structure of arabidopsis NPR1 : its function as a salicylic acid receptor and a metal-binding protein .” 2011. Thesis, Brock University. Accessed September 16, 2019. http://hdl.handle.net/10464/3422.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zhang, Di. “The structure of arabidopsis NPR1 : its function as a salicylic acid receptor and a metal-binding protein .” 2011. Web. 16 Sep 2019.

Vancouver:

Zhang D. The structure of arabidopsis NPR1 : its function as a salicylic acid receptor and a metal-binding protein . [Internet] [Thesis]. Brock University; 2011. [cited 2019 Sep 16]. Available from: http://hdl.handle.net/10464/3422.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zhang D. The structure of arabidopsis NPR1 : its function as a salicylic acid receptor and a metal-binding protein . [Thesis]. Brock University; 2011. Available from: http://hdl.handle.net/10464/3422

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rutgers University

18. Wang, Haoxun, 1989-. Regulation of renal organic anion transporters.

Degree: PhD, Pharmaceutical Science, 2019, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/60932/

►

Organic anion transporters (OATs) are a group of membrane proteins that are mainly involved in the body disposition of organic anionic molecules or zwitterions, including… (more)

Subjects/Keywords: Kidneys – Molecular aspects; Anions

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APA (6^th Edition):

Wang, Haoxun, 1. (2019). Regulation of renal organic anion transporters. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/60932/

Chicago Manual of Style (16^th Edition):

Wang, Haoxun, 1989-. “Regulation of renal organic anion transporters.” 2019. Doctoral Dissertation, Rutgers University. Accessed September 16, 2019. https://rucore.libraries.rutgers.edu/rutgers-lib/60932/.

MLA Handbook (7^th Edition):

Wang, Haoxun, 1989-. “Regulation of renal organic anion transporters.” 2019. Web. 16 Sep 2019.

Vancouver:

Wang, Haoxun 1. Regulation of renal organic anion transporters. [Internet] [Doctoral dissertation]. Rutgers University; 2019. [cited 2019 Sep 16]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/60932/.

Council of Science Editors:

Wang, Haoxun 1. Regulation of renal organic anion transporters. [Doctoral Dissertation]. Rutgers University; 2019. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/60932/

University of Hong Kong

19. Bahl, Justin. Molecular evolution of three morphologically similar families in the Xylariomycetidae (Apiosporaceae, Clypeosphaeriaceae, Hyponectriaceae).

Degree: PhD, 2006, University of Hong Kong

URL: Bahl, J.. (2006). Molecular evolution of three morphologically similar families in the Xylariomycetidae (Apiosporaceae, Clypeosphaeriaceae, Hyponectriaceae). (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b3785674 ; http://dx.doi.org/10.5353/th_b3785674 ; http://hdl.handle.net/10722/51007

published_or_final_version

abstract

Ecology and Biodiversity

Doctoral

Doctor of Philosophy

Subjects/Keywords: Ascomycetes - Molecular aspects.; Amphisphaeriaceae - Molecular aspects.; Xylariales - Molecular aspects.

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APA (6^th Edition):

Bahl, J. (2006). Molecular evolution of three morphologically similar families in the Xylariomycetidae (Apiosporaceae, Clypeosphaeriaceae, Hyponectriaceae). (Doctoral Dissertation). University of Hong Kong. Retrieved from Bahl, J.. (2006). Molecular evolution of three morphologically similar families in the Xylariomycetidae (Apiosporaceae, Clypeosphaeriaceae, Hyponectriaceae). (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b3785674 ; http://dx.doi.org/10.5353/th_b3785674 ; http://hdl.handle.net/10722/51007

Chicago Manual of Style (16^th Edition):

Bahl, Justin. “Molecular evolution of three morphologically similar families in the Xylariomycetidae (Apiosporaceae, Clypeosphaeriaceae, Hyponectriaceae).” 2006. Doctoral Dissertation, University of Hong Kong. Accessed September 16, 2019. Bahl, J.. (2006). Molecular evolution of three morphologically similar families in the Xylariomycetidae (Apiosporaceae, Clypeosphaeriaceae, Hyponectriaceae). (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b3785674 ; http://dx.doi.org/10.5353/th_b3785674 ; http://hdl.handle.net/10722/51007.

MLA Handbook (7^th Edition):

Bahl, Justin. “Molecular evolution of three morphologically similar families in the Xylariomycetidae (Apiosporaceae, Clypeosphaeriaceae, Hyponectriaceae).” 2006. Web. 16 Sep 2019.

Vancouver:

Bahl J. Molecular evolution of three morphologically similar families in the Xylariomycetidae (Apiosporaceae, Clypeosphaeriaceae, Hyponectriaceae). [Internet] [Doctoral dissertation]. University of Hong Kong; 2006. [cited 2019 Sep 16]. Available from: Bahl, J.. (2006). Molecular evolution of three morphologically similar families in the Xylariomycetidae (Apiosporaceae, Clypeosphaeriaceae, Hyponectriaceae). (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b3785674 ; http://dx.doi.org/10.5353/th_b3785674 ; http://hdl.handle.net/10722/51007.

Council of Science Editors:

Bahl J. Molecular evolution of three morphologically similar families in the Xylariomycetidae (Apiosporaceae, Clypeosphaeriaceae, Hyponectriaceae). [Doctoral Dissertation]. University of Hong Kong; 2006. Available from: Bahl, J.. (2006). Molecular evolution of three morphologically similar families in the Xylariomycetidae (Apiosporaceae, Clypeosphaeriaceae, Hyponectriaceae). (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b3785674 ; http://dx.doi.org/10.5353/th_b3785674 ; http://hdl.handle.net/10722/51007

Jawaharlal Nehru University

20. Rao, S Chenchal. Molecular aspects of the eye lens and its proteins; -.

Degree: Centre for Cellular and Molecular Biology, 1991, Jawaharlal Nehru University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/32211

Available

References p.86-199

Advisors/Committee Members: Balasubramanlan, D.

Subjects/Keywords: Molecular aspects; proteins

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APA (6^th Edition):

Rao, S. C. (1991). Molecular aspects of the eye lens and its proteins; -. (Thesis). Jawaharlal Nehru University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/32211

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rao, S Chenchal. “Molecular aspects of the eye lens and its proteins; -.” 1991. Thesis, Jawaharlal Nehru University. Accessed September 16, 2019. http://shodhganga.inflibnet.ac.in/handle/10603/32211.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rao, S Chenchal. “Molecular aspects of the eye lens and its proteins; -.” 1991. Web. 16 Sep 2019.

Vancouver:

Rao SC. Molecular aspects of the eye lens and its proteins; -. [Internet] [Thesis]. Jawaharlal Nehru University; 1991. [cited 2019 Sep 16]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/32211.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rao SC. Molecular aspects of the eye lens and its proteins; -. [Thesis]. Jawaharlal Nehru University; 1991. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/32211

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University

21. Slauson, Marjorie E. Tumor targeting with a ⁹⁹̳mTcMAG-3 labeled molecular engine.

Degree: MS, Radiation Health Physics, 2006, Oregon State University

URL: http://hdl.handle.net/1957/28773

► A unique tumor targeted method, which may be able to deliver a molecule to the surface of a tumor cell using the pH gradient between… (more)

Subjects/Keywords: Tumors – Molecular aspects

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APA (6^th Edition):

Slauson, M. E. (2006). Tumor targeting with a ⁹⁹̳mTcMAG-3 labeled molecular engine. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/28773

Chicago Manual of Style (16^th Edition):

Slauson, Marjorie E. “Tumor targeting with a ⁹⁹̳mTcMAG-3 labeled molecular engine.” 2006. Masters Thesis, Oregon State University. Accessed September 16, 2019. http://hdl.handle.net/1957/28773.

MLA Handbook (7^th Edition):

Slauson, Marjorie E. “Tumor targeting with a ⁹⁹̳mTcMAG-3 labeled molecular engine.” 2006. Web. 16 Sep 2019.

Vancouver:

Slauson ME. Tumor targeting with a ⁹⁹̳mTcMAG-3 labeled molecular engine. [Internet] [Masters thesis]. Oregon State University; 2006. [cited 2019 Sep 16]. Available from: http://hdl.handle.net/1957/28773.

Council of Science Editors:

Slauson ME. Tumor targeting with a ⁹⁹̳mTcMAG-3 labeled molecular engine. [Masters Thesis]. Oregon State University; 2006. Available from: http://hdl.handle.net/1957/28773

University of Hong Kong

22. 刘谨; Liu, Jin. The role of exchange protein directly activated by cyclic AMP 1-deficiency in diabetic and ischemic retinopathy.

Degree: M. Phil., 2011, University of Hong Kong

URL: Liu, J. [刘谨]. (2011). The role of exchange protein directly activated by cyclic AMP 1-deficiency in diabetic and ischemic retinopathy. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4731015 ; http://dx.doi.org/10.5353/th_b4731015 ; http://hdl.handle.net/10722/174370

► Previous in vitro studies showed that exchange protein directly activated by cyclic AMP 1 (Epac1), which is a cAMP mediator, plays an important role in… (more)

▼ Previous in vitro studies showed that exchange protein directly activated by cyclic AMP 1 (Epac1), which is a cAMP mediator, plays an important role in maintenance of endothelial barrier function. Diabetic retinopathy is characterized by impairment of retinal blood vessel integrity leading to breakdown of blood retinal barrier, retinal hypoxia, and neuronal damage. Here, we hypothesize that Epac1 regulates endothelial permeability and protects retina from the retinal damage associated with diabetes. To test such hypothesis, we first demonstrated that human retinal microvascular endothelial cells (HRMECs) exposed to high glucose concentration at 25 mM or 35 mM showed the decreased Epac1 expression level. Our preliminary data also showed that Epac1-downstream activator, Rap1, a member of Ras GTPase, was also altered by different glucose levels. In addition, retina from type 2 diabetic, db/db, mice also showed the decreased Epac1 expression compared to that of non-diabetic, db/m, mice. To further determine the role of Epac1 in diabetic retinopathy, we made use of Epac1-deficient mice. The pathogenesis of diabetic retinopathy share similar characteristics to that of ischemic retinopathy, such as neuronal cell death, glial reactivity, and glutamate toxicity. Therefore, we used our previous retinal ischemic model, i.e., transient middle cerebral artery occlusion (tMCAO). Firstly, we determined the retinal morphology of Epac1-/- mice under normal condition at 3wks. At 3 wks old, the Epac1-/- retinae showed a significantly decreased thickness of outer plexiform layer (OPL) with a trend of increase in inner nuclear layer (INL) thickness. Interestingly, there were obviously more glutamine synthetase (GS)-positive M?ller cells and protein kinase C (PKC)-α positive rod bipolar cells in INL. In addition, there were more IgG-positive blood vessels in OPL. To further determine whether these phenotypes will lead to more severe retinal damage, Epac1-/- mice were exposed to 2 hours of MCAO followed by 22 hours of reperfusion, which we have previously shown to induce retinal ischemia. There was no obvious difference in retinal thickness and expressions of glial fibrillary acidic protein (GFAP) and GS in the contralateral sides of Epac1+/+ and Epac1-/- retina after tMCAO suggesting that the Epac1-deficiency may be compensated by either protein kinase A (PKA) or Epac2. However, Epac2 level was not altered by Epac1-deficiency by Western blot analysis. The ipsilateral sides of the retina of Epac1+/+ and Epac1-/- after tMCAO also did not show obvious difference in swelling and cell death in inner retina, GFAP, glutamate, GS, nitrotyrosine (NT), and peroxiredoxin 6 (Prx6), suggesting that Epac1-deficiency may have been compensated by other cAMP mediators, such as Epac2. However, Epac2 expression in the ipsilateral side of Epac1+/+ and Epac1-/- retinae was not significantly different, although the activities of Epac and PKA were not determined. Taken together, the Epac1-deficient mice would serve as a useful model to determine the role of… Advisors/Committee Members: Chung, SK.

Subjects/Keywords: Cyclic adenylic acid.; Diabetic retinopathy - Molecular aspects.

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APA (6^th Edition):

刘谨; Liu, J. (2011). The role of exchange protein directly activated by cyclic AMP 1-deficiency in diabetic and ischemic retinopathy. (Masters Thesis). University of Hong Kong. Retrieved from Liu, J. [刘谨]. (2011). The role of exchange protein directly activated by cyclic AMP 1-deficiency in diabetic and ischemic retinopathy. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4731015 ; http://dx.doi.org/10.5353/th_b4731015 ; http://hdl.handle.net/10722/174370

Chicago Manual of Style (16^th Edition):

刘谨; Liu, Jin. “The role of exchange protein directly activated by cyclic AMP 1-deficiency in diabetic and ischemic retinopathy.” 2011. Masters Thesis, University of Hong Kong. Accessed September 16, 2019. Liu, J. [刘谨]. (2011). The role of exchange protein directly activated by cyclic AMP 1-deficiency in diabetic and ischemic retinopathy. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4731015 ; http://dx.doi.org/10.5353/th_b4731015 ; http://hdl.handle.net/10722/174370.

MLA Handbook (7^th Edition):

刘谨; Liu, Jin. “The role of exchange protein directly activated by cyclic AMP 1-deficiency in diabetic and ischemic retinopathy.” 2011. Web. 16 Sep 2019.

Vancouver:

刘谨; Liu J. The role of exchange protein directly activated by cyclic AMP 1-deficiency in diabetic and ischemic retinopathy. [Internet] [Masters thesis]. University of Hong Kong; 2011. [cited 2019 Sep 16]. Available from: Liu, J. [刘谨]. (2011). The role of exchange protein directly activated by cyclic AMP 1-deficiency in diabetic and ischemic retinopathy. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4731015 ; http://dx.doi.org/10.5353/th_b4731015 ; http://hdl.handle.net/10722/174370.

Council of Science Editors:

刘谨; Liu J. The role of exchange protein directly activated by cyclic AMP 1-deficiency in diabetic and ischemic retinopathy. [Masters Thesis]. University of Hong Kong; 2011. Available from: Liu, J. [刘谨]. (2011). The role of exchange protein directly activated by cyclic AMP 1-deficiency in diabetic and ischemic retinopathy. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4731015 ; http://dx.doi.org/10.5353/th_b4731015 ; http://hdl.handle.net/10722/174370

University of Hong Kong

23. Sam, Laiju. Molecular biology of a cryptic dehalogenase from Burkholderia cepacia MBA4.

Degree: PhD, 2000, University of Hong Kong

URL: http://hdl.handle.net/10722/26199

►

(Uncorrected OCR) Abstract of the thesis entitled MOLECULAR BIOLOGY OF A CRYPTIC DEHALOGENASE FROM Burkholderia cepacia MBA4 Submitted by Laiju Sam For the degree of… (more)

▼

(Uncorrected OCR) Abstract of the thesis entitled MOLECULAR BIOLOGY OF A CRYPTIC DEHALOGENASE FROM Burkholderia cepacia MBA4 Submitted by Laiju Sam For the degree of Doctor of Philosophy at The University of Hong Kong in July 2000 Burkholderia cepacia MBA4 has been previously shown to produce a single dehalogenase in batch culture condition. Other cryptic dehalogenases were detected in cells grown in continuous culture. In this study, one of the cryptic dehalogenases was cloned and characterised. This cryptic haloacid dehalogenase was designated Chd 1 and expressed constitutively in Escherichia coli. The structural gene, chdJ, was isolated from a 1.7-kb PstI fragment. This fragment. contains a functional promoter since the expression of chdl in E. coli is orientation independent. The nucleotide sequence of the fragment was determined and analysed and an open reading frame for 840 amino acids was identified. The nucleotide sequence of chdl did not show any homology with those of other dehalogenase genes. Comparison of the deduced amino acid sequence, however, showed significant homology, ranging from 42-50%, with the amino acid sequences of several other dehalogenases. Phylogenetic analysis indicated that Chd 1 is closely related to dehalogenase CI and dehalogenase IVa of Pseudomonas putida CBS3 and B. cepacia MBA4 respectively. 1 The recombinant Chdl had a native molecular weight of 58,000 daltons whereas the denatured molecular weight was found to be 27,000 daltons. Thus, the enzyme exists predominantly as a dimer. When the activity towards the two stereoisomers of 2-monochloropropionic acid were considered individually, the enzyme was found to be active towards the L-isomer only. The purified enzyme was most active towards monobromoacetic acid with specific activities of 5.44, 1.5, 4.56, and 1.95 J!mole of halide released/min/mg protein for monobromoacetic acid, 2-monobromopropionic acid, monochloroacetic acid and 2-monochloropropionic acid respectively. Maximum activity of the enzyme was observed at pH 6.5. The enzyme was found to be thermolabile. chdl was cloned in the T7 RNA polymerase driven pRSET A and Irc promoter based pPROEXHT expression vectors. However, no significant expression of chdl was obtained in these systems. Chd 1 differed from other dehalogenases in having a long leader sequence. This leader sequence contains a potential signal peptidase cleavage site. This is a property of periplasmic enzymes. In order to detect the unprocessed molecules of Chd 1, chdl was expressed in the temperature-sensitive E. coli strain IT 41 (lep9-mutant). This strain contains a mutation in the leader peptidase gene. The leader peptidase is inactive in this strain at the non-permissive temperature of o 42 C. Precursor molecules of Chd 1 was detected in cultures shifted to this non- permissive temperature. Periplasmic fractions isolated from E. coli harboring chdl were found to contain the active dehalogenase. 11

abstract

toc

Botany

Doctoral

Doctor of Philosophy

Subjects/Keywords: Pseudomonas - Molecular aspects.

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APA (6^th Edition):

Sam, L. (2000). Molecular biology of a cryptic dehalogenase from Burkholderia cepacia MBA4. (Doctoral Dissertation). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/26199

Chicago Manual of Style (16^th Edition):

Sam, Laiju. “Molecular biology of a cryptic dehalogenase from Burkholderia cepacia MBA4.” 2000. Doctoral Dissertation, University of Hong Kong. Accessed September 16, 2019. http://hdl.handle.net/10722/26199.

MLA Handbook (7^th Edition):

Sam, Laiju. “Molecular biology of a cryptic dehalogenase from Burkholderia cepacia MBA4.” 2000. Web. 16 Sep 2019.

Vancouver:

Sam L. Molecular biology of a cryptic dehalogenase from Burkholderia cepacia MBA4. [Internet] [Doctoral dissertation]. University of Hong Kong; 2000. [cited 2019 Sep 16]. Available from: http://hdl.handle.net/10722/26199.

Council of Science Editors:

Sam L. Molecular biology of a cryptic dehalogenase from Burkholderia cepacia MBA4. [Doctoral Dissertation]. University of Hong Kong; 2000. Available from: http://hdl.handle.net/10722/26199

University of Hong Kong

24. Ngai, Cheung. Usefulness of molecular targets for identification of medically important tsukamurella species.

Degree: Master of Medical Sciences, 2009, University of Hong Kong

URL: Ngai, C. [魏祥]. (2009). Usefulness of molecular targets for identification of medically important tsukamurella species. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4292530 ; http://dx.doi.org/10.5353/th_b4292530 ; http://hdl.handle.net/10722/56700

published_or_final_version

Microbiology

Master

Master of Medical Sciences

Subjects/Keywords: Corynebacterium - Molecular aspects.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ngai, C. (2009). Usefulness of molecular targets for identification of medically important tsukamurella species. (Masters Thesis). University of Hong Kong. Retrieved from Ngai, C. [魏祥]. (2009). Usefulness of molecular targets for identification of medically important tsukamurella species. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4292530 ; http://dx.doi.org/10.5353/th_b4292530 ; http://hdl.handle.net/10722/56700

Chicago Manual of Style (16^th Edition):

Ngai, Cheung. “Usefulness of molecular targets for identification of medically important tsukamurella species.” 2009. Masters Thesis, University of Hong Kong. Accessed September 16, 2019. Ngai, C. [魏祥]. (2009). Usefulness of molecular targets for identification of medically important tsukamurella species. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4292530 ; http://dx.doi.org/10.5353/th_b4292530 ; http://hdl.handle.net/10722/56700.

MLA Handbook (7^th Edition):

Ngai, Cheung. “Usefulness of molecular targets for identification of medically important tsukamurella species.” 2009. Web. 16 Sep 2019.

Vancouver:

Ngai C. Usefulness of molecular targets for identification of medically important tsukamurella species. [Internet] [Masters thesis]. University of Hong Kong; 2009. [cited 2019 Sep 16]. Available from: Ngai, C. [魏祥]. (2009). Usefulness of molecular targets for identification of medically important tsukamurella species. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4292530 ; http://dx.doi.org/10.5353/th_b4292530 ; http://hdl.handle.net/10722/56700.

Council of Science Editors:

Ngai C. Usefulness of molecular targets for identification of medically important tsukamurella species. [Masters Thesis]. University of Hong Kong; 2009. Available from: Ngai, C. [魏祥]. (2009). Usefulness of molecular targets for identification of medically important tsukamurella species. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4292530 ; http://dx.doi.org/10.5353/th_b4292530 ; http://hdl.handle.net/10722/56700

University of Hong Kong

25. Pan, Qing. Functions and physiological significance of the N- and C- terminal regions of the Escherichia coli global transcription factor FNR.

Degree: PhD, 2013, University of Hong Kong

URL: Pan, Q. [潘庆]. (2013). Functions and physiological significance of the N- and C- terminal regions of the Escherichia coli global transcription factor FNR. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4985852 ; http://dx.doi.org/10.5353/th_b4985852 ; http://hdl.handle.net/10722/196081

► A facultative anaerobe such as Escherichia coli is able to switch between the aerobic and anaerobic modes of metabolism in response to O2 availability. This… (more)

▼ A facultative anaerobe such as Escherichia coli is able to switch between the aerobic and anaerobic modes of metabolism in response to O2 availability. This adaptation is primarily controlled by a global transcription regulator called FNR (fumarate nitrate reduction). The key property that allows FNR to act as an O2 responsive transcription factor is its capability to dimerize and being activated upon binding of an O2 labile [4Fe-4S]2+ cluster. Previous functional studies have largely focused on the regions of FNR analogous to CRP (cAMP receptor protein), a prototype CRP/FNR family protein which X-ray crystal structure has been resolved. However, E. coli FNR contains extra N- and C-terminal regions that are conserved among various FNR orthologs but are absent in CRP. The functions of these two regions have not been resolved. In this study, their functions and physiological significance to the O2 sensing capacity of FNR were systematically investigated. A three-alanine (3-Ala) scanning library on amino acid 2-19 and 236-250 of FNR was constructed and selective 3-Ala substitution mutants exhibited variable defects. These defects were found to be due to their impairment of intracellular FNR protein levels which was unique only among FNR mutations in these two regions. Introduction of 3-Ala substitution at the residues 239-244, resulting in LAQ239-241A3 and LAG242-244A3 respectively, caused an especially accelerated degradation and decrease of intracellular FNR proteins. These variants were found to be degraded by the ClpXP protease. Sequence alignment of FNR orthologs revealed a highly conserved “L239XXL242XG244” motif, and my experimental data further revealed that L239 and L242 were important residues and were responsible for the defects of LAQ239-241A3 and LAG242-244A3, respectively. Circular dichroism analysis revealed that introduction of LAQ239-241A3 caused conformational changes with a significant loss of secondary structures in FNR. These studies taken together suggest that the N- and C-terminal regions of FNR play an important role in mediating the intracellular protein level of FNR. My studies also specified the ClpXP signals as the N-terminal RR9-10 and C-terminal VA249-250, and indicated that VA249-250 is a more important site than RR9-10 in targeting FNR to proteolysis. The second topic of the thesis involves exploration of the regulatory mechanism of an anaerobically activated multidrug efflux pump MdtEF in E. coli. MdtEF is an important multidrug efflux pump that causes antibiotic resistance upon overexpression. Previous studies revealed that expression of MdtEF was significantly upregulated under anaerobic conditions, but its regulatory mechanism was unknown. In the current study, systematic analyses on the unusually long promoter region of the gadE-mdtEF operon which drives the expression of MdtEF were performed. It was found that unlike FNR, mdtEF was not regulated at post-translational level by proteolysis, but at transcriptional level through the promoter region of gadE. My study showed that…

Subjects/Keywords: Transcription factors; Escherichia coli - Molecular aspects

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pan, Q. (2013). Functions and physiological significance of the N- and C- terminal regions of the Escherichia coli global transcription factor FNR. (Doctoral Dissertation). University of Hong Kong. Retrieved from Pan, Q. [潘庆]. (2013). Functions and physiological significance of the N- and C- terminal regions of the Escherichia coli global transcription factor FNR. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4985852 ; http://dx.doi.org/10.5353/th_b4985852 ; http://hdl.handle.net/10722/196081

Chicago Manual of Style (16^th Edition):

Pan, Qing. “Functions and physiological significance of the N- and C- terminal regions of the Escherichia coli global transcription factor FNR.” 2013. Doctoral Dissertation, University of Hong Kong. Accessed September 16, 2019. Pan, Q. [潘庆]. (2013). Functions and physiological significance of the N- and C- terminal regions of the Escherichia coli global transcription factor FNR. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4985852 ; http://dx.doi.org/10.5353/th_b4985852 ; http://hdl.handle.net/10722/196081.

MLA Handbook (7^th Edition):

Pan, Qing. “Functions and physiological significance of the N- and C- terminal regions of the Escherichia coli global transcription factor FNR.” 2013. Web. 16 Sep 2019.

Vancouver:

Pan Q. Functions and physiological significance of the N- and C- terminal regions of the Escherichia coli global transcription factor FNR. [Internet] [Doctoral dissertation]. University of Hong Kong; 2013. [cited 2019 Sep 16]. Available from: Pan, Q. [潘庆]. (2013). Functions and physiological significance of the N- and C- terminal regions of the Escherichia coli global transcription factor FNR. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4985852 ; http://dx.doi.org/10.5353/th_b4985852 ; http://hdl.handle.net/10722/196081.

Council of Science Editors:

Pan Q. Functions and physiological significance of the N- and C- terminal regions of the Escherichia coli global transcription factor FNR. [Doctoral Dissertation]. University of Hong Kong; 2013. Available from: Pan, Q. [潘庆]. (2013). Functions and physiological significance of the N- and C- terminal regions of the Escherichia coli global transcription factor FNR. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4985852 ; http://dx.doi.org/10.5353/th_b4985852 ; http://hdl.handle.net/10722/196081

University of Hong Kong

26. 程天凡.; Cheng, Tianfan. Structural and functional aspects of the multifaceted SlyD in Helicobacter pylori.

Degree: PhD, 2012, University of Hong Kong

URL: Cheng, T. [程天凡]. (2012). Structural and functional aspects of the multifaceted SlyD in Helicobacter pylori. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4819919 ; http://dx.doi.org/10.5353/th_b4819919 ; http://hdl.handle.net/10722/188237

►

As a ubiquitous protein-folding helper in bacterial cytosol, SlyD is a peptidylprolyl isomerase (PPIase) of the FK506-binding protein (FKBP) family. It has two important functional… (more)

▼

As a ubiquitous protein-folding helper in bacterial cytosol, SlyD is a peptidylprolyl isomerase (PPIase) of the FK506-binding protein (FKBP) family. It has two important functional domains, the IF (insert-in-flap) domain with chaperone activity and the FKBP domain with PPIase activity. It also possesses a histidine- and cysteine-rich C-terminal metal-binding domain, which binds to selected divalent metal ions (e.g. Ni2+, Zn2+) and is critical for participation in metal trafficking for metalloenzymes. SlyD from Helicobacter pylori was investigated both structurally and functionally by a variety of biophysical, biochemical and molecular biology techniques. HpSlyD was cloned, expressed and purified. It binds to Ni2+ and Zn2+ with dissociation constants (Kd) of 2.74 and 3.79 μM, respectively. Both Ni2+ and Zn2+ can competitively bind to HpSlyD. The C-terminus was demonstrated to convey nickel resistance in vivo. It also binds to Bi3+ with Kd of 4.4 × 10-24 M. Furthermore, Zn2+, Cu2+ and Bi3+ can induce the dimerization or oligomerization of HpSlyD. The solution structure of the C-terminus-truncated SlyD from Helicobacter pylori (HpSlyDΔC) was determined by NMR, which demonstrates that HpSlyDΔC folds into two well-separated, orientation-independent domains. Both the FKBP and IF domains fold into a structure consisting of a four-stranded antiparallel β-sheet and an α-helix. Binding of Ni2+ instead of Zn2+ induced the conformational changes in FKBP domain, where the active sites are positioned, suggesting a regulatory role of nickel on the function of HpSlyD. It was also confirmed that HpSlyD can associate with the Tat (twin-arginine translocation) signal peptide from small subunit of [NiFe] hydrogenase (HydA), an accessory protein HpHypB for [NiFe] hydrogenase mainly by the IF domain. Surprisingly HpSlyD was found to form a complex with HpUreE, a urease chaperone, indicative of the “cross-talk” between [NiFe] hydrogenase and urease. The possible mechanism of HpSlyD for the cooperation with HpHypB was also explored. In the presence of different metal ions, HpSlyD was shown to regulate the GTPase activity of HpHypB, implicating the possible metal transfer induced by HpSlyD. It was suggested that HpSlyD modulates the nickel insertion of [NiFe] hydrogenase by controlling the GTPase activity of HpHypB. In this thesis, the SlyD protein from H. pylori was shown as an important regulator for the activation of both [NiFe] hydrogenase and urease.

published_or_final_version

Chemistry

Doctoral

Doctor of Philosophy

Subjects/Keywords: Helicobacter pylori - Molecular aspects.; Protein binding.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

程天凡.; Cheng, T. (2012). Structural and functional aspects of the multifaceted SlyD in Helicobacter pylori. (Doctoral Dissertation). University of Hong Kong. Retrieved from Cheng, T. [程天凡]. (2012). Structural and functional aspects of the multifaceted SlyD in Helicobacter pylori. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4819919 ; http://dx.doi.org/10.5353/th_b4819919 ; http://hdl.handle.net/10722/188237

Chicago Manual of Style (16^th Edition):

程天凡.; Cheng, Tianfan. “Structural and functional aspects of the multifaceted SlyD in Helicobacter pylori.” 2012. Doctoral Dissertation, University of Hong Kong. Accessed September 16, 2019. Cheng, T. [程天凡]. (2012). Structural and functional aspects of the multifaceted SlyD in Helicobacter pylori. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4819919 ; http://dx.doi.org/10.5353/th_b4819919 ; http://hdl.handle.net/10722/188237.

MLA Handbook (7^th Edition):

程天凡.; Cheng, Tianfan. “Structural and functional aspects of the multifaceted SlyD in Helicobacter pylori.” 2012. Web. 16 Sep 2019.

Vancouver:

程天凡.; Cheng T. Structural and functional aspects of the multifaceted SlyD in Helicobacter pylori. [Internet] [Doctoral dissertation]. University of Hong Kong; 2012. [cited 2019 Sep 16]. Available from: Cheng, T. [程天凡]. (2012). Structural and functional aspects of the multifaceted SlyD in Helicobacter pylori. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4819919 ; http://dx.doi.org/10.5353/th_b4819919 ; http://hdl.handle.net/10722/188237.

Council of Science Editors:

程天凡.; Cheng T. Structural and functional aspects of the multifaceted SlyD in Helicobacter pylori. [Doctoral Dissertation]. University of Hong Kong; 2012. Available from: Cheng, T. [程天凡]. (2012). Structural and functional aspects of the multifaceted SlyD in Helicobacter pylori. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4819919 ; http://dx.doi.org/10.5353/th_b4819919 ; http://hdl.handle.net/10722/188237

University of Hong Kong

27. 林映辰.; Lin, Ying Chen. Functional analysis of anther-specific genes essential for pollen exine development and male fertility in tobacco.

Degree: M. Phil., 2012, University of Hong Kong

URL: Lin, Y. C. [林映辰]. (2012). Functional analysis of anther-specific genes essential for pollen exine development and male fertility in tobacco. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5053417 ; http://dx.doi.org/10.5353/th_b5053417 ; http://hdl.handle.net/10722/188298

►

In flowering plants, pollen grains are surrounded by extremely strong outer walls providing solid and firm structure for protecting pollen and species-specific interactions with female… (more)

▼

In flowering plants, pollen grains are surrounded by extremely strong outer walls providing solid and firm structure for protecting pollen and species-specific interactions with female stigma. The outer wall of pollen, referred to as exine, is composed of sporopollenin polymer, but the composition and synthesis of sporopollenin remains poorly understood. Previous studies have indicated that several genes such as Fatty Acyl-CoA Synthetase (ACOS5), Polyketide Synthases (PKSA and PKSB), and Tetraketide α-Pyrone Reductase (TKPR1) take part in the biosynthesis of sporopollenin in Arabidopsis thaliana. The existence of ancient biochemical pathways for sporopollenin biosynthesis has been widely proposed but experimental evidence from plant species other than Arabidopsis is not extensively available. In this study, two homologous PKS genes, NtPKS1 and NtPKS2, were found in tobacco (Nicotiana tabacum). Results of RT-PCR and in situ hybridization revealed that NtPKS1 and NtPKS2 are specifically and transiently expressed in tapetal cells during microspore development in tobacco anthers. RNAi plants of NtACOS1 and NtPKS1 were investigated. Comparing with wild-type tobacco (SR1), abnormal pollens, defect exine structure, and male sterility were found in the RNAi lines. Enzymatic assays show that NtPKS1 and NtPKS2 encode anther-specific enzymes using fatty acyl-coenzyme A and p-coumaroyl coenzyme A as substrates to yield tri- and tetra- ketide α-pyrone and bisnoryangonin respectively. In this study, the metabolic steps catalyzed by the anther-specific acyl- CoA synthetase (ACOS), polyketide synthase (PKS), and tetraketide α-pyrone reductase (TKPR) were investigated. Using fatty acids as starting substrates, sequential activities of heterologously-expressed tobacco enzymes NtACOS1, NtPKS1, and NtTKPR1 resulted in the production of reduced tetraketide α- pyrones which propose to contribute to the biosynthesis of sporopollenin precursors in tobacco.

published_or_final_version

Biological Sciences

Master

Master of Philosophy

Advisors/Committee Members: Lo, CSC.

Subjects/Keywords: Tobacco - Reproduction - Molecular aspects.; Tobacco - Genetics.

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APA (6^th Edition):

林映辰.; Lin, Y. C. (2012). Functional analysis of anther-specific genes essential for pollen exine development and male fertility in tobacco. (Masters Thesis). University of Hong Kong. Retrieved from Lin, Y. C. [林映辰]. (2012). Functional analysis of anther-specific genes essential for pollen exine development and male fertility in tobacco. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5053417 ; http://dx.doi.org/10.5353/th_b5053417 ; http://hdl.handle.net/10722/188298

Chicago Manual of Style (16^th Edition):

林映辰.; Lin, Ying Chen. “Functional analysis of anther-specific genes essential for pollen exine development and male fertility in tobacco.” 2012. Masters Thesis, University of Hong Kong. Accessed September 16, 2019. Lin, Y. C. [林映辰]. (2012). Functional analysis of anther-specific genes essential for pollen exine development and male fertility in tobacco. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5053417 ; http://dx.doi.org/10.5353/th_b5053417 ; http://hdl.handle.net/10722/188298.

MLA Handbook (7^th Edition):

林映辰.; Lin, Ying Chen. “Functional analysis of anther-specific genes essential for pollen exine development and male fertility in tobacco.” 2012. Web. 16 Sep 2019.

Vancouver:

林映辰.; Lin YC. Functional analysis of anther-specific genes essential for pollen exine development and male fertility in tobacco. [Internet] [Masters thesis]. University of Hong Kong; 2012. [cited 2019 Sep 16]. Available from: Lin, Y. C. [林映辰]. (2012). Functional analysis of anther-specific genes essential for pollen exine development and male fertility in tobacco. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5053417 ; http://dx.doi.org/10.5353/th_b5053417 ; http://hdl.handle.net/10722/188298.

Council of Science Editors:

林映辰.; Lin YC. Functional analysis of anther-specific genes essential for pollen exine development and male fertility in tobacco. [Masters Thesis]. University of Hong Kong; 2012. Available from: Lin, Y. C. [林映辰]. (2012). Functional analysis of anther-specific genes essential for pollen exine development and male fertility in tobacco. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5053417 ; http://dx.doi.org/10.5353/th_b5053417 ; http://hdl.handle.net/10722/188298

University of Hong Kong

28. Hung, Wing-yan. The role of TAX1BP2 in hepatocellular carcinoma.

Degree: M. Phil., 2012, University of Hong Kong

URL: Hung, W. [洪穎欣]. (2012). The role of TAX1BP2 in hepatocellular carcinoma. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4852155 ; http://dx.doi.org/10.5353/th_b4852155 ; http://hdl.handle.net/10722/193070

► TAX1 Binding Protein 2 (TAX1BP2) has been found to be a centrosome duplication regulating protein. Previous findings have demonstrated that over-expression of TAX1BP2 suppresses centrosome… (more)

▼ TAX1 Binding Protein 2 (TAX1BP2) has been found to be a centrosome duplication regulating protein. Previous findings have demonstrated that over-expression of TAX1BP2 suppresses centrosome over-duplication. Recently, our lab has revealed that TAX1BP2 is a novel tumor suppressor in hepatocellular carcinoma (HCC) regulated by cyclin-dependent protein kinase 2 (CDK2), nevertheless, the molecular mechanism of how TAX1BP2 regulates centrosome duplication and the link between its centrosome duplication regulatory ability and the tumor suppressing property remain elusive. With the aim to understand the roles of TAX1BP2 in HCC, the present study intended to investigate the link between centrosome duplication regulating ability and tumor suppressing property. Polo-like kinase 4 (PLK4) is a special member of the Polo-like kinase family as its structure is diverged from other family members. Instead of having two Polo-boxes, it carries one Polo-box and one cryptic Polo-box. It has been shown that PLK4 is involved in the formation of centrioles, an important component of centrosome, and is a key regulator of centrosome duplication. Based on the functional similarity, it was hypothesized that PLK4 may function as a regulator of TAX1BP2. To define if PLK4 regulate TAX1BP2, the interaction between PLK4 and TAX1BP2, both in vivo and in vitro, was first confirmed using affinity pulldown and co-immunoprecipitation assays. To understand the significance of the physical interaction, in vitro and in vivo kinase assay were used to study the phosphorylation activity between PLK4 and TAX1BP2. It was demonstrated that TAX1BP2 is a potential substrate of PLK4. Centrosome duplication assay was also performed to investigate if over-expression of PLK4 abolished the centrosome over-duplication suppressing ability of TAX1BP2. In order to delineate the signaling pathway of TAX1BP2, the interaction between TAX1BP2 and its cellular interacting partners was investigated in this study. Ten proteins were isolated as potential interacting partners of TAX1BP2 using Tandem affinity purification (TAP) coupled with Mass Spectrometry protein fingerprinting. Two of the ten proteins, the Ezrin and Mortalin, were confirmed to be binding partners of TAX1BP2 using affinity pull-down assay and TAP, respectively. The identification of the interacting partners suggested that TAX1BP2 may modulate centrosome duplication via alteration of the subcellular localization of Mortalin. These findings helped to delineate the signaling pathway of TAX1BP2 and enabled the better understanding of the roles of TAX1BP2 in tumor suppressor function of HCC. In summary, we demonstrated that TAX1BP2 contains a centrosome duplication regulatory domain (CDRD) and its centrosome duplication regulating ability is critical for its tumor suppressing property. Moreover, three novel interacting partners of TAX1BP2, including Ezrin, PLK4 and Mortalin, are identified. Our findings provide a new insight into the roles of TAX1BP2 in centrosome duplication, hepatocarcinogenesis and…

Subjects/Keywords: Tumor suppressor proteins; Liver - Cancer - Molecular aspects

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hung, W. (2012). The role of TAX1BP2 in hepatocellular carcinoma. (Masters Thesis). University of Hong Kong. Retrieved from Hung, W. [洪穎欣]. (2012). The role of TAX1BP2 in hepatocellular carcinoma. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4852155 ; http://dx.doi.org/10.5353/th_b4852155 ; http://hdl.handle.net/10722/193070

Chicago Manual of Style (16^th Edition):

Hung, Wing-yan. “The role of TAX1BP2 in hepatocellular carcinoma.” 2012. Masters Thesis, University of Hong Kong. Accessed September 16, 2019. Hung, W. [洪穎欣]. (2012). The role of TAX1BP2 in hepatocellular carcinoma. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4852155 ; http://dx.doi.org/10.5353/th_b4852155 ; http://hdl.handle.net/10722/193070.

MLA Handbook (7^th Edition):

Hung, Wing-yan. “The role of TAX1BP2 in hepatocellular carcinoma.” 2012. Web. 16 Sep 2019.

Vancouver:

Hung W. The role of TAX1BP2 in hepatocellular carcinoma. [Internet] [Masters thesis]. University of Hong Kong; 2012. [cited 2019 Sep 16]. Available from: Hung, W. [洪穎欣]. (2012). The role of TAX1BP2 in hepatocellular carcinoma. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4852155 ; http://dx.doi.org/10.5353/th_b4852155 ; http://hdl.handle.net/10722/193070.

Council of Science Editors:

Hung W. The role of TAX1BP2 in hepatocellular carcinoma. [Masters Thesis]. University of Hong Kong; 2012. Available from: Hung, W. [洪穎欣]. (2012). The role of TAX1BP2 in hepatocellular carcinoma. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4852155 ; http://dx.doi.org/10.5353/th_b4852155 ; http://hdl.handle.net/10722/193070

University of Hong Kong

29. 单越; Shan, Yue. Molecular engineering of the Escherichia coli global transcription factor FNR to improve its stability to oxygen.

Degree: M. Phil., 2012, University of Hong Kong

URL: Shan, Y. [单越]. (2012). Molecular engineering of the Escherichia coli global transcription factor FNR to improve its stability to oxygen. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4961737 ; http://dx.doi.org/10.5353/th_b4961737 ; http://hdl.handle.net/10722/193014

► The ability to sense and rapidly respond to oxygen availability is crucial to the survival and physiology of facultative anaerobes. In many gram negative bacteria… (more)

▼ The ability to sense and rapidly respond to oxygen availability is crucial to the survival and physiology of facultative anaerobes. In many gram negative bacteria such as Escherichia coli, this process is primarily controlled by the dimeric, [4Fe〖-4S]〗^(2+) containing global transcription factor FNR, which regulates transcription of genes necessary for the anaerobic metabolism. Activity of FNR is directly regulated by the presence of oxygen, which inactivates FNR by oxidizing the [4Fe〖-4S]〗^(2+) cluster and causing the dissociation of the FNR dimer. Although the biological function of FNR has been well established, structural and biochemical characterization of the FNR dimer has been limited due to its extreme lability to oxygen. In the current study, I conduct molecular engineering on FNR protein and obtain oxygen stable variants that are suitable for in vitro biochemical studies. By combining several approaches including covalently linking two FNR monomers using a flexible peptide linker, amino acid substitutions to promote dimerization, and removal of protease recognition sites to prevent proteolysis, a series of FNR variants which are potentially active in the presence of oxygen are constructed. Various in vivo and in vitro assays led to the identification of the construct (FNRD154A)2 which covalently links two copies of FNRD154A, an FNR variant that has greater dimerization capability, in tandem displays significantly improved transcription regulation and DNA binding to various FNR regulated promoters in the presence of O2. Circular Dichroism analysis showed that this variant maintains a similar secondary structure as that of native FNRD154A and in vivo transcription assay demonstrated that this protein retains other properties of the native FNR dimer including [4Fe〖-4S]〗^(2+) cluster binding, oxygen sensing, and capability to support the anaerobic growth of E. coli. All these together led the conclusion that an FNR variant that retains structural and functional properties of native FNR has been constructed, but with significantly improved O2 stability. Thus, it has the potential to be widely used in various biochemical and structural studies of FNR in the presence of oxygen. In addition to the major project of molecular engineering of FNR protein, in this thesis, I also initiated the study of using metabolomics approaches to identify the cellular substrates of the multidrug efflux pump MdtEF. MdtEF is an important efflux pump in E. coli and its expression has been shown to be induced under a number of stressed conditions. It is thus proposed to have a general detoxification function in E. coli, but the cellular substrates it expels have not been identified. In this study we established and applied metabolite profiling on the wild type and ΔmdtEF E. coli strains and confirmed that indole red, a metabolic by-product formed during anaerobic respiration of nitrate, is one of the cellular substrates of MdtEF under anaerobic conditions. This study provides a general methodology to identify endogenous substrates…

Subjects/Keywords: Transcription factors; Escherichia coli - Molecular aspects

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

单越; Shan, Y. (2012). Molecular engineering of the Escherichia coli global transcription factor FNR to improve its stability to oxygen. (Masters Thesis). University of Hong Kong. Retrieved from Shan, Y. [单越]. (2012). Molecular engineering of the Escherichia coli global transcription factor FNR to improve its stability to oxygen. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4961737 ; http://dx.doi.org/10.5353/th_b4961737 ; http://hdl.handle.net/10722/193014

Chicago Manual of Style (16^th Edition):

单越; Shan, Yue. “Molecular engineering of the Escherichia coli global transcription factor FNR to improve its stability to oxygen.” 2012. Masters Thesis, University of Hong Kong. Accessed September 16, 2019. Shan, Y. [单越]. (2012). Molecular engineering of the Escherichia coli global transcription factor FNR to improve its stability to oxygen. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4961737 ; http://dx.doi.org/10.5353/th_b4961737 ; http://hdl.handle.net/10722/193014.

MLA Handbook (7^th Edition):

单越; Shan, Yue. “Molecular engineering of the Escherichia coli global transcription factor FNR to improve its stability to oxygen.” 2012. Web. 16 Sep 2019.

Vancouver:

单越; Shan Y. Molecular engineering of the Escherichia coli global transcription factor FNR to improve its stability to oxygen. [Internet] [Masters thesis]. University of Hong Kong; 2012. [cited 2019 Sep 16]. Available from: Shan, Y. [单越]. (2012). Molecular engineering of the Escherichia coli global transcription factor FNR to improve its stability to oxygen. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4961737 ; http://dx.doi.org/10.5353/th_b4961737 ; http://hdl.handle.net/10722/193014.

Council of Science Editors:

单越; Shan Y. Molecular engineering of the Escherichia coli global transcription factor FNR to improve its stability to oxygen. [Masters Thesis]. University of Hong Kong; 2012. Available from: Shan, Y. [单越]. (2012). Molecular engineering of the Escherichia coli global transcription factor FNR to improve its stability to oxygen. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4961737 ; http://dx.doi.org/10.5353/th_b4961737 ; http://hdl.handle.net/10722/193014

University of Hong Kong

30. Wong, Wai-fung. Structural characterization of N-terminus of XIAP associated factor 1.

Degree: M. Phil., 2011, University of Hong Kong

URL: Wong, W. [黃偉鋒]. (2011). Structural characterization of N-terminus of XIAP associated factor 1. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4833003 ; http://dx.doi.org/10.5353/th_b4833003 ; http://hdl.handle.net/10722/173873

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The main focus of this thesis is to study the physical characteristics of the N-terminus of human XAF1 protein, a 17kD protein named NTA1, by… (more)

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The main focus of this thesis is to study the physical characteristics of the N-terminus of human XAF1 protein, a 17kD protein named NTA1, by biophysical methods. Structural studies of the N-terminus of XAF1 serves as a base for the studies of the structure and function relationship of the N-terminus, and the same maybe true for the full length XAF1. Bioinformatics analysis shows that NTA1 shares high sequence identity with the TRAF-type zinc finger domain-containing protein 1 (TRAFD1) and FLN29. Protein structure prediction has been performed on NTA1 by the I-TASSER web server. The prediction result suggests that NTA1 is a structure that consists of α-helices which are joined by flexible linkers. The loose structure shown by I-TASSER is expected to have high solvent accessibility. This coincides with the deuterium exchange data. In addition, by the CD approach, NTA1 was estimated to contain high α-helix content. This result is consistent with the bioinformatics prediction and the secondary structure obtained from the chemical shift index method as well. The physical characterizations of NTA1 showed that NTA1 is a loosely packed protein; and the five zinc ions are bound in the protein structure. Based on the chemical shifts of β-carbons, the Cysteine residues Cys8, Cys11, Cys34, Cys37, Cys50, Cys59, Cys62, Cys86, Cys89 and Cys115 showed a significantly downfield shift, they are probably involved in the zinc ions coordination. The dynamic property of NTA1 was investigated by NMR techniques. Backbone dynamics of NTA1 reveal that NTA1 does not have a typical spherical structure, it is anisotropic. Residues corresponding to the zinc finger regions in the predicted structure show large R2/R1 and S2 values, while regions shown to be flexible linkers in the 3D structure prediction show small R2/R1 and S2 values. Thus, the protein structure homology modeling data are supported by the backbone dynamics data.

published_or_final_version

Chemistry

Master

Master of Philosophy

Advisors/Committee Members: Sun, H, Sze, KH.

Subjects/Keywords: Apoptosis - Molecular aspects.; Zinc-finger proteins.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wong, W. (2011). Structural characterization of N-terminus of XIAP associated factor 1. (Masters Thesis). University of Hong Kong. Retrieved from Wong, W. [黃偉鋒]. (2011). Structural characterization of N-terminus of XIAP associated factor 1. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4833003 ; http://dx.doi.org/10.5353/th_b4833003 ; http://hdl.handle.net/10722/173873

Chicago Manual of Style (16^th Edition):

Wong, Wai-fung. “Structural characterization of N-terminus of XIAP associated factor 1.” 2011. Masters Thesis, University of Hong Kong. Accessed September 16, 2019. Wong, W. [黃偉鋒]. (2011). Structural characterization of N-terminus of XIAP associated factor 1. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4833003 ; http://dx.doi.org/10.5353/th_b4833003 ; http://hdl.handle.net/10722/173873.

MLA Handbook (7^th Edition):

Wong, Wai-fung. “Structural characterization of N-terminus of XIAP associated factor 1.” 2011. Web. 16 Sep 2019.

Vancouver:

Wong W. Structural characterization of N-terminus of XIAP associated factor 1. [Internet] [Masters thesis]. University of Hong Kong; 2011. [cited 2019 Sep 16]. Available from: Wong, W. [黃偉鋒]. (2011). Structural characterization of N-terminus of XIAP associated factor 1. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4833003 ; http://dx.doi.org/10.5353/th_b4833003 ; http://hdl.handle.net/10722/173873.

Council of Science Editors:

Wong W. Structural characterization of N-terminus of XIAP associated factor 1. [Masters Thesis]. University of Hong Kong; 2011. Available from: Wong, W. [黃偉鋒]. (2011). Structural characterization of N-terminus of XIAP associated factor 1. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4833003 ; http://dx.doi.org/10.5353/th_b4833003 ; http://hdl.handle.net/10722/173873

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