subject:( molecular aspects)

Oregon State University

1. Codding, Sara Jone. Functional and Structural Analyses of Three Distinct proteins by Biochemical and Biophysical Techniques.

Degree: PhD, Biochemistry & Biophysics, 2016, Oregon State University

URL: http://hdl.handle.net/1957/60138

► The focus of this dissertation was to understand protein function and structure on a molecular level. To do this successfully, a variety of biochemical and… (more)

▼ The focus of this dissertation was to understand protein function and structure on a molecular level. To do this successfully, a variety of biochemical and biophysical techniques were employed. A wide variety of techniques were applied to study the proteins of interest in this dissertation to probe protein-protein interactions, protein function, protein structure, and protein regulation. In addition, the use of bioinformatics has placed the similarities and differences of these proteins compared to homologs in perspective within their protein families. There are three chapters of original research presented here, the second chapter is published, the third contains my contribution to a work that is published, and the fourth will be submitted for publication. The second chapter of this thesis describes the studies on the protein dysferlin. Mutations in dysferlin are responsible for late onset muscular dystrophies where muscle cells are deficient in resealing the cell membrane upon routine injury. We demonstrate that dysferlin catalyzes the rate-limiting step of membrane fusion and mediates lipid mixing in a calcium sensitive manner. This work was the first in the field to determine a mechanism of action of dysferlin and suggests a molecular basis for why mutations that abrogate dysferlin's activity result in muscular dystrophy. The third chapter includes my contribution to the complete structural solution of the enzyme ValA involved in the synthesis of the crop protectant validamycin A. This was the first structure of a sedoheputlose-7-phoshpate cyclase, and the overall fold reflects that of similar sugar cyclase proteins. But, the sequence and structural analysis of ValA with respect to other related proteins revealed the first novel theory regarding substrate specificity of ValA. It is postulated that due to sequence alignment and structural data, ValA selectively binds the β-anomer of its substrate which pre-assigns stereochemistry of the enzymatic product. In the fourth chapter of this dissertation we design several variants of the protein merlin involved in cell contact growth inhibition. Whereas a complete structural solution was never obtained for merlin in this study, we used in vitro techniques to test the role of merlin's central domain in the conformational regulation of the protein. This was the first study on merlin, or the homologous proteins ezrin, radixin, and moesin, that focused on the role of the central domain. Our results confirm that this domain is not merely structural, but mediates key interactions that modulate merlin's degree of intramolecular association. That merlin's activity is central to cell contact growth inhibition and that this activity is modulated by merlin's degree of intramolecular association, it is clear now that the central domain plays a key role in the activity of merlin. In the final chapter and fifth chapter I conclude this dissertation. Advisors/Committee Members: Johnson, Colin (advisor), Taylor, Barbara J (committee member).

Subjects/Keywords: dysferlin; Muscular dystrophy – Molecular aspects

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APA (6^th Edition):

Codding, S. J. (2016). Functional and Structural Analyses of Three Distinct proteins by Biochemical and Biophysical Techniques. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/60138

Chicago Manual of Style (16^th Edition):

Codding, Sara Jone. “Functional and Structural Analyses of Three Distinct proteins by Biochemical and Biophysical Techniques.” 2016. Doctoral Dissertation, Oregon State University. Accessed January 19, 2021. http://hdl.handle.net/1957/60138.

MLA Handbook (7^th Edition):

Codding, Sara Jone. “Functional and Structural Analyses of Three Distinct proteins by Biochemical and Biophysical Techniques.” 2016. Web. 19 Jan 2021.

Vancouver:

Codding SJ. Functional and Structural Analyses of Three Distinct proteins by Biochemical and Biophysical Techniques. [Internet] [Doctoral dissertation]. Oregon State University; 2016. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1957/60138.

Council of Science Editors:

Codding SJ. Functional and Structural Analyses of Three Distinct proteins by Biochemical and Biophysical Techniques. [Doctoral Dissertation]. Oregon State University; 2016. Available from: http://hdl.handle.net/1957/60138

Oregon State University

2. Creason, Allison L. Characterization of the pathogenicity and phylogeny of members of the Rhodococcus genus.

Degree: PhD, Molecular and Cellular Biology, 2015, Oregon State University

URL: http://hdl.handle.net/1957/55812

► Plant pathogenic Rhodococcus species are persistent pathogens able to cause severe growth deformities on a large range of hosts. The most well studied species, R.… (more)

Subjects/Keywords: Rhodococcus – Phylogeny – Molecular aspects

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APA (6^th Edition):

Creason, A. L. (2015). Characterization of the pathogenicity and phylogeny of members of the Rhodococcus genus. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/55812

Chicago Manual of Style (16^th Edition):

Creason, Allison L. “Characterization of the pathogenicity and phylogeny of members of the Rhodococcus genus.” 2015. Doctoral Dissertation, Oregon State University. Accessed January 19, 2021. http://hdl.handle.net/1957/55812.

MLA Handbook (7^th Edition):

Creason, Allison L. “Characterization of the pathogenicity and phylogeny of members of the Rhodococcus genus.” 2015. Web. 19 Jan 2021.

Vancouver:

Creason AL. Characterization of the pathogenicity and phylogeny of members of the Rhodococcus genus. [Internet] [Doctoral dissertation]. Oregon State University; 2015. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1957/55812.

Council of Science Editors:

Creason AL. Characterization of the pathogenicity and phylogeny of members of the Rhodococcus genus. [Doctoral Dissertation]. Oregon State University; 2015. Available from: http://hdl.handle.net/1957/55812

Oregon State University

3. Njuguna, Wambui. Development and use of molecular tools in Fragaria.

Degree: PhD, Horticulture, 2010, Oregon State University

URL: http://hdl.handle.net/1957/15096

► This dissertation describes the application and development of molecular tools that have great potential for use in studying variation in strawberry germplasm. The first study… (more)

▼ This dissertation describes the application and development of molecular tools that have great potential for use in studying variation in strawberry germplasm. The first study evaluated 91 microsatellite (simple sequence repeat, SSR) markers for transferability in 22 Fragaria species and their utility in fingerprinting octoploid strawberries. Out of the transferable markers, a reduced set of four SSRs was developed based on polymorphism, multiplexing ability, reproducibility and ease of scoring. Unique SSR fingerprints were obtained for over 175 Fragaria samples representing 22 Fragaria species used in the study. Testing of two molecular markers linked to the red stele and anthracnose resistances identified potential sources of resistance in previously untested genotypes. Further characterization of these accessions is warranted to validate resistance and usefulness in breeding. In the second study, 20 SSRs polymorphic in wild Asian diploids, F. iinumae and F. nipponica, from Hokkaido, Japan were selected for genetic analysis of 137 accessions from 22 locations. Principal coordinate analysis followed by non-parametric modal clustering grouped accessions into two groups representing the two species. Further clustering within the groups resulted in 10 groups (7-F. iinumae, 3-F. nipponica) that suggest lineages representative of the diversity in Hokkaido, Japan. The third study tested plant DNA barcodes, the nuclear ribosomal Internal Transcribed and the chloroplast PsbA-trnH spacers, for Fragaria species identification. The 'barcoding gap', between within species and between species variation, was absent and prevented identification of Fragaria species. The fourth study evaluated the genetic diversity of 94 accessions representing 22 Fragaria species using four universal chloroplast SSR loci. Genetic diversity was moderate (0.54) despite the homoplasy observed. Species-specific haplotypes for F. nipponica, F. orientalis, F. iinumae and F. nilgerrensis were identified. Sequencing whole chloroplast genomes using Illumina in a final study revealed a close maternal genome relationship between F. vesca ssp. bracteata and the octoploid species supporting a North American origin of the octoploids and the polyphyly of F. vesca. Calculation of divergence time of Fragaria revealed young evolutionary age of the genus at 2.7 million years and of the octoploids at 450,000 years. Advisors/Committee Members: Bassil, Nahla V. (advisor), Mehlenbacher, Shawn (committee member).

Subjects/Keywords: strawberries; Strawberries – Molecular aspects

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APA (6^th Edition):

Njuguna, W. (2010). Development and use of molecular tools in Fragaria. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/15096

Chicago Manual of Style (16^th Edition):

Njuguna, Wambui. “Development and use of molecular tools in Fragaria.” 2010. Doctoral Dissertation, Oregon State University. Accessed January 19, 2021. http://hdl.handle.net/1957/15096.

MLA Handbook (7^th Edition):

Njuguna, Wambui. “Development and use of molecular tools in Fragaria.” 2010. Web. 19 Jan 2021.

Vancouver:

Njuguna W. Development and use of molecular tools in Fragaria. [Internet] [Doctoral dissertation]. Oregon State University; 2010. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1957/15096.

Council of Science Editors:

Njuguna W. Development and use of molecular tools in Fragaria. [Doctoral Dissertation]. Oregon State University; 2010. Available from: http://hdl.handle.net/1957/15096

Oregon State University

4. Kepler, Ryan M. Advances in molecular systematics of clavicipitaceous fungi (Sordariomycetes: Hypocreales).

Degree: PhD, Botany and Plant Pathology, 2010, Oregon State University

URL: http://hdl.handle.net/1957/19589

► Historical concepts of Clavicipitaceae have included a broad range of species that display diverse morphologies, ecological modes and host associations. When subjected to multigene phylogenetic… (more)

▼ Historical concepts of Clavicipitaceae have included a broad range of species that display diverse morphologies, ecological modes and host associations. When subjected to multigene phylogenetic investigation of evolutionary history, the family was found to be polyphyletic, largely driven by diversity in the genus Cordyceps, previously containing over 400 taxa. The majority of Cordyceps sensu lato now resides in the families Cordycipitaceae, for which the genus Cordyceps has been retained owing to the placement of the type C. militaris, and the genera Ophiocordyceps and Elaphocordyceps in Ophiocordycipitaceae. The genus Metacordyceps was defined for species of Cordyceps remaining in Clavicipitaceae sensu stricto and contains relatively few species owing to convergent morphologies. Clavicipitaceae remains considerably diverse and its members attack hosts across three kingdoms of life, including insects and rotifers, plants and other fungi. There remains a significant number of Cordyceps species for which molecular or morphological data are insufficient, and are therefore considered incertae sedis with regards to family until new material is available for examination. This work expands the sampling of taxa in Clavicipitaceae sensu lato for inclusion in phylogenetic reconstruction, with particular emphasis on residual species of Cordyceps. The resulting phylogenies were then used to refine concepts morphological features that define boundaries between taxa and explore the evolution of host association, morphology and ecology with ancestral character-state reconstruction. Taxon sampling was increased by extensive field collections and collaborations with other researchers. The diversity present in newly acquired specimens represents all three pathogen groups. The inclusion of these taxa in a five-gene dataset (nrSSU, nrLSU, TEF, RPB1, RPB2) advances understanding of systematic relationships within Clavicipitaceae. Molecular data supports the movement of seven species from Cordyceps sensu lato to Metacordyceps, doubling the number of species known for the genus. The addition of new species reveals significant structure within the genus and allows for a reevaluation and strengthening of morphological concepts attributed to the genus. A clade of species sister to Clavicipitaceae was identified as Veterocordyceps gen. nov. This finding provides clarity to the results of previous workers who considered species in the genus as members of Ophiocordyceps based on morphological data and a more limited molecular dataset. Cordyceps fratricida, a pathogen of ergot, and the rice pathogen Ustilaginoidea virens were placed phylogenetically within Clavicipitaceae. The genus Tyrranocordyceps gen. nov. was established for fungal pathogens that attack closely related species of ergot. Tyrranocordyceps represents a sexual state for a clade of fungal pathogens known only from asexual states. Ustilaginoidea virens was found to occupy a separate lineage of plant pathogens, representing a third clade of clavicipitaceous fungi deriving their… Advisors/Committee Members: Spatafora, Joseph W. (advisor), Muir, Patricia (committee member).

Subjects/Keywords: Fungi; Hypocreales – Phylogeny – Molecular aspects

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APA (6^th Edition):

Kepler, R. M. (2010). Advances in molecular systematics of clavicipitaceous fungi (Sordariomycetes: Hypocreales). (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/19589

Chicago Manual of Style (16^th Edition):

Kepler, Ryan M. “Advances in molecular systematics of clavicipitaceous fungi (Sordariomycetes: Hypocreales).” 2010. Doctoral Dissertation, Oregon State University. Accessed January 19, 2021. http://hdl.handle.net/1957/19589.

MLA Handbook (7^th Edition):

Kepler, Ryan M. “Advances in molecular systematics of clavicipitaceous fungi (Sordariomycetes: Hypocreales).” 2010. Web. 19 Jan 2021.

Vancouver:

Kepler RM. Advances in molecular systematics of clavicipitaceous fungi (Sordariomycetes: Hypocreales). [Internet] [Doctoral dissertation]. Oregon State University; 2010. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1957/19589.

Council of Science Editors:

Kepler RM. Advances in molecular systematics of clavicipitaceous fungi (Sordariomycetes: Hypocreales). [Doctoral Dissertation]. Oregon State University; 2010. Available from: http://hdl.handle.net/1957/19589

East Carolina University

5. Williams, Melinda. Analysis of the hex regulon in Pseudomonas aeruginosa.

Degree: 2012, East Carolina University

URL: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=13782

► Pseudomonas aeruginosa is an opportunistic pathogen whose defense mechanisms include biofilm production through carbohydrate metabolism. The activity of the enzymes in the hex regulon are… (more)

Subjects/Keywords: Pseudomonas aeruginosa – Molecular aspects

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APA (6^th Edition):

Williams, M. (2012). Analysis of the hex regulon in Pseudomonas aeruginosa. (Masters Thesis). East Carolina University. Retrieved from http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=13782

Chicago Manual of Style (16^th Edition):

Williams, Melinda. “Analysis of the hex regulon in Pseudomonas aeruginosa.” 2012. Masters Thesis, East Carolina University. Accessed January 19, 2021. http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=13782.

MLA Handbook (7^th Edition):

Williams, Melinda. “Analysis of the hex regulon in Pseudomonas aeruginosa.” 2012. Web. 19 Jan 2021.

Vancouver:

Williams M. Analysis of the hex regulon in Pseudomonas aeruginosa. [Internet] [Masters thesis]. East Carolina University; 2012. [cited 2021 Jan 19]. Available from: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=13782.

Council of Science Editors:

Williams M. Analysis of the hex regulon in Pseudomonas aeruginosa. [Masters Thesis]. East Carolina University; 2012. Available from: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=13782

University of North Carolina – Greensboro

6. Stacy, Anderson Timothy IV. Efforts towards the formal synthesis of bakuchiol via small molecule manipulation.

Degree: 2014, University of North Carolina – Greensboro

URL: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=16616

► Bakuchiol is a monoterpinoid compound found mainly in the seeds of Psoralea coryfolia Linn, which is indigenous to Southeast Asia. Since its discovery and isolation… (more)

Subjects/Keywords: Psoralea – Molecular aspects; Biosynthesis

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APA (6^th Edition):

Stacy, A. T. I. (2014). Efforts towards the formal synthesis of bakuchiol via small molecule manipulation. (Masters Thesis). University of North Carolina – Greensboro. Retrieved from http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=16616

Chicago Manual of Style (16^th Edition):

Stacy, Anderson Timothy IV. “Efforts towards the formal synthesis of bakuchiol via small molecule manipulation.” 2014. Masters Thesis, University of North Carolina – Greensboro. Accessed January 19, 2021. http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=16616.

MLA Handbook (7^th Edition):

Stacy, Anderson Timothy IV. “Efforts towards the formal synthesis of bakuchiol via small molecule manipulation.” 2014. Web. 19 Jan 2021.

Vancouver:

Stacy ATI. Efforts towards the formal synthesis of bakuchiol via small molecule manipulation. [Internet] [Masters thesis]. University of North Carolina – Greensboro; 2014. [cited 2021 Jan 19]. Available from: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=16616.

Council of Science Editors:

Stacy ATI. Efforts towards the formal synthesis of bakuchiol via small molecule manipulation. [Masters Thesis]. University of North Carolina – Greensboro; 2014. Available from: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=16616

Brock University

7. Zhang, Di. The structure of arabidopsis NPR1 : its function as a salicylic acid receptor and a metal-binding protein .

Degree: Centre for Biotechnology, 2011, Brock University

URL: http://hdl.handle.net/10464/3422

► The Arabidopsis NPRI protein regulates systemic acquired resistance dependent on salicylic acid. Analyses by plant two-hybrid analysis in vivo and pull-down assays in vitro showed… (more)

Subjects/Keywords: Arabidopsis – Molecular aspects.; Metalloproteins

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APA (6^th Edition):

Zhang, D. (2011). The structure of arabidopsis NPR1 : its function as a salicylic acid receptor and a metal-binding protein . (Thesis). Brock University. Retrieved from http://hdl.handle.net/10464/3422

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zhang, Di. “The structure of arabidopsis NPR1 : its function as a salicylic acid receptor and a metal-binding protein .” 2011. Thesis, Brock University. Accessed January 19, 2021. http://hdl.handle.net/10464/3422.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zhang, Di. “The structure of arabidopsis NPR1 : its function as a salicylic acid receptor and a metal-binding protein .” 2011. Web. 19 Jan 2021.

Vancouver:

Zhang D. The structure of arabidopsis NPR1 : its function as a salicylic acid receptor and a metal-binding protein . [Internet] [Thesis]. Brock University; 2011. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10464/3422.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zhang D. The structure of arabidopsis NPR1 : its function as a salicylic acid receptor and a metal-binding protein . [Thesis]. Brock University; 2011. Available from: http://hdl.handle.net/10464/3422

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Massey University

8. Rehman, Zahid ur. Molecular mechanism of export of alginate in Pseudomonas aeruginosa : a thesis presented in partial fulfilment of the requirements for [the] degree of Doctor of Philosophy in Microbiology at Massey University, New Zealand .

Degree: 2013, Massey University

URL: http://hdl.handle.net/10179/4322

► Pseudomonas aeruginosa is an opportunistic pathogen; infecting insects, plants and humans. It is of particular relevance to cystic fibrosis (CF) patients where it causes pulmonary… (more)

Subjects/Keywords: Pseudomonas aeruginosa; Molecular aspects; Alginates

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APA (6^th Edition):

Rehman, Z. u. (2013). Molecular mechanism of export of alginate in Pseudomonas aeruginosa : a thesis presented in partial fulfilment of the requirements for [the] degree of Doctor of Philosophy in Microbiology at Massey University, New Zealand . (Thesis). Massey University. Retrieved from http://hdl.handle.net/10179/4322

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rehman, Zahid ur. “Molecular mechanism of export of alginate in Pseudomonas aeruginosa : a thesis presented in partial fulfilment of the requirements for [the] degree of Doctor of Philosophy in Microbiology at Massey University, New Zealand .” 2013. Thesis, Massey University. Accessed January 19, 2021. http://hdl.handle.net/10179/4322.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rehman, Zahid ur. “Molecular mechanism of export of alginate in Pseudomonas aeruginosa : a thesis presented in partial fulfilment of the requirements for [the] degree of Doctor of Philosophy in Microbiology at Massey University, New Zealand .” 2013. Web. 19 Jan 2021.

Vancouver:

Rehman Zu. Molecular mechanism of export of alginate in Pseudomonas aeruginosa : a thesis presented in partial fulfilment of the requirements for [the] degree of Doctor of Philosophy in Microbiology at Massey University, New Zealand . [Internet] [Thesis]. Massey University; 2013. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10179/4322.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rehman Zu. Molecular mechanism of export of alginate in Pseudomonas aeruginosa : a thesis presented in partial fulfilment of the requirements for [the] degree of Doctor of Philosophy in Microbiology at Massey University, New Zealand . [Thesis]. Massey University; 2013. Available from: http://hdl.handle.net/10179/4322

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rutgers University

9. Wang, Haoxun, 1989-. Regulation of renal organic anion transporters.

Degree: PhD, Pharmaceutical Science, 2019, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/60932/

►

Organic anion transporters (OATs) are a group of membrane proteins that are mainly involved in the body disposition of organic anionic molecules or zwitterions, including… (more)

Subjects/Keywords: Kidneys – Molecular aspects; Anions

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APA (6^th Edition):

Wang, Haoxun, 1. (2019). Regulation of renal organic anion transporters. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/60932/

Chicago Manual of Style (16^th Edition):

Wang, Haoxun, 1989-. “Regulation of renal organic anion transporters.” 2019. Doctoral Dissertation, Rutgers University. Accessed January 19, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/60932/.

MLA Handbook (7^th Edition):

Wang, Haoxun, 1989-. “Regulation of renal organic anion transporters.” 2019. Web. 19 Jan 2021.

Vancouver:

Wang, Haoxun 1. Regulation of renal organic anion transporters. [Internet] [Doctoral dissertation]. Rutgers University; 2019. [cited 2021 Jan 19]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/60932/.

Council of Science Editors:

Wang, Haoxun 1. Regulation of renal organic anion transporters. [Doctoral Dissertation]. Rutgers University; 2019. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/60932/

Rutgers University

10. Patrizii, Michele. Post-translational modifications on the K2 domain modulate SOX9 transcriptional activity in lung cancer.

Degree: PhD, Pharmacology, Cellular and Molecular, 2019, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/61905/

►

Lung cancer is the deadliest cancer type, accounting for about 25% of all cancer-related deaths. Despite impressive advancements in the molecular characterization of lung cancer,… (more)

Subjects/Keywords: Lungs – Cancer – Molecular aspects

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Patrizii, M. (2019). Post-translational modifications on the K2 domain modulate SOX9 transcriptional activity in lung cancer. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/61905/

Chicago Manual of Style (16^th Edition):

Patrizii, Michele. “Post-translational modifications on the K2 domain modulate SOX9 transcriptional activity in lung cancer.” 2019. Doctoral Dissertation, Rutgers University. Accessed January 19, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/61905/.

MLA Handbook (7^th Edition):

Patrizii, Michele. “Post-translational modifications on the K2 domain modulate SOX9 transcriptional activity in lung cancer.” 2019. Web. 19 Jan 2021.

Vancouver:

Patrizii M. Post-translational modifications on the K2 domain modulate SOX9 transcriptional activity in lung cancer. [Internet] [Doctoral dissertation]. Rutgers University; 2019. [cited 2021 Jan 19]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/61905/.

Council of Science Editors:

Patrizii M. Post-translational modifications on the K2 domain modulate SOX9 transcriptional activity in lung cancer. [Doctoral Dissertation]. Rutgers University; 2019. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/61905/

11. NC DOCKS at The University of North Carolina at Greensboro; Stacy, Anderson Timothy. Efforts towards the formal synthesis of bakuchiol via small molecule manipulation.

Degree: 2014, NC Docks

URL: http://libres.uncg.edu/ir/uncg/f/StacyIV_uncg_0154M_11518.pdf

► Bakuchiol is a monoterpinoid compound found mainly in the seeds of Psoralea coryfolia Linn, which is indigenous to Southeast Asia. Since its discovery and isolation… (more)

Subjects/Keywords: Psoralea $x Molecular aspects; Biosynthesis

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APA (6^th Edition):

NC DOCKS at The University of North Carolina at Greensboro; Stacy, A. T. (2014). Efforts towards the formal synthesis of bakuchiol via small molecule manipulation. (Thesis). NC Docks. Retrieved from http://libres.uncg.edu/ir/uncg/f/StacyIV_uncg_0154M_11518.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

NC DOCKS at The University of North Carolina at Greensboro; Stacy, Anderson Timothy. “Efforts towards the formal synthesis of bakuchiol via small molecule manipulation.” 2014. Thesis, NC Docks. Accessed January 19, 2021. http://libres.uncg.edu/ir/uncg/f/StacyIV_uncg_0154M_11518.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

NC DOCKS at The University of North Carolina at Greensboro; Stacy, Anderson Timothy. “Efforts towards the formal synthesis of bakuchiol via small molecule manipulation.” 2014. Web. 19 Jan 2021.

Vancouver:

NC DOCKS at The University of North Carolina at Greensboro; Stacy AT. Efforts towards the formal synthesis of bakuchiol via small molecule manipulation. [Internet] [Thesis]. NC Docks; 2014. [cited 2021 Jan 19]. Available from: http://libres.uncg.edu/ir/uncg/f/StacyIV_uncg_0154M_11518.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

NC DOCKS at The University of North Carolina at Greensboro; Stacy AT. Efforts towards the formal synthesis of bakuchiol via small molecule manipulation. [Thesis]. NC Docks; 2014. Available from: http://libres.uncg.edu/ir/uncg/f/StacyIV_uncg_0154M_11518.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Stellenbosch University

12. Vergotine, Zelda. Molecular investigation of genetic factors associated with insulin resistance and obesity in a South African population.

Degree: PhD, Pathology, 2015, Stellenbosch University

URL: http://hdl.handle.net/10019.1/97967

► ENGLISH ABSTRACT: Background: The aetiopathogenesis of type 2 diabetes and the associated insulin resistance have been shown to have a strong genetic basis. Several genetic… (more)

▼ ENGLISH ABSTRACT: Background: The aetiopathogenesis of type 2 diabetes and the associated insulin resistance have been shown to have a strong genetic basis. Several genetic variants of the peroxisome proliferatoractivated receptor gamma (PPARG) and the insulin receptor substrate (IRS) 1 genes have been associated with the metabolic states of obesity, insulin resistance and type 2 diabetes in Caucasian populations. Furthermore, insulin resistance is strongly associated with diabetes and subsequent cardiovascular disease. These are increasingly common in low- to middle -income countries, including South Africa. Limited information is currently available regarding genetic associations with insulin resistance in African populations. Objectives: (1) To identify subjects with insulin resistance and determine the frequencies of the single nucleotide polymorphisms in the PPARG and IRS1 genes and examine the associated risk of insulin resistance and type 2 diabetes mellitus in a mixed-ancestry South African population. (2) To investigate the relationship between indices of insulin resistance and carotid intima media thickness, a marker of subclinical cardiovascular disease/atherosclerosis. Methods: A total of 856 (235 males) mixed-ancestry adults drawn from an urban community of Bellville South, Cape Town were genotyped for PPARG Pro12Ala (rs1801282, G>C), Pro115Gln (rs1800571, G>T), Val290Met (rs72551362, G>A), Pheu388Leu (rs72551363, T>A), Arg397Cys (rs72551364, C>T), His449His (rs3856806, C>T) and IRS1 Gly972Arg (rs 1801278, G>A). The oral glucose tolerance test was performed and cardiometabolic risk factors measured. Insulin resistance was estimated by the homeostasis model assessment of insulin resistance, the homeostasis model assessment of functional beta-cells, the quantitative insulin-sensitivity check index, the fasting insulin resistance index and the glucose/insulin ratio. Carotid intima media thickness was measured in longitudinal section at the far wall of the distal common carotid arteries, 2 cm from the bifurcation, at three consecutive end-points, 5-10 mm apart. Results: The genotype frequencies of PPARG Pro12Ala, IRS1 Gly972Arg and PPARG His449His were 10,4%, 7,7% and 23,8% respectively. No mutations were found for PPARG Pro115Gln, Val290Met, Pheu388Leu and Arg379Cys. In a model containing both PPARG Pro12Ala and IRS1 Gly972Arg alleles and their interaction term, the presence of the PPARG Pro12 resulted in a 64% risk of prevalent type 2 diabetes mellitus and was associated with higher 2 hour post-OGTT insulin levels in subjects with normoglycaemia. The PPARG Pro12 was associated with insulin resistance and interacted with IRS1 Gly972Arg, increasing the risk of type 2 diabetes mellitus. The PPARG His449His allele T frequency was about 14% and in an additive genetic model significantly reduced the risk of diabetes by 44%. After adjustment for age, gender, body mass index and diabetes status, the fasting plasma glucose (β=0,087;p=0,042) and glucose/insulin ratio (β=0,026; p=0,026)… Advisors/Committee Members: Erasmus, Rajiv T., Matsha, Tandi E., Pillay, Tahir S., Kotze, Maritha, Stellenbosch University. Faculty of Medicine and Health Sciences. Dept. of Pathology: Chemical Pathology..

Subjects/Keywords: Diabetes – Genetic aspects; Obesity – Genetic aspects; Diabetes – Molecular aspects; Obesity – Molecular aspects; Insulin resistance; UCTD

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APA (6^th Edition):

Vergotine, Z. (2015). Molecular investigation of genetic factors associated with insulin resistance and obesity in a South African population. (Doctoral Dissertation). Stellenbosch University. Retrieved from http://hdl.handle.net/10019.1/97967

Chicago Manual of Style (16^th Edition):

Vergotine, Zelda. “Molecular investigation of genetic factors associated with insulin resistance and obesity in a South African population.” 2015. Doctoral Dissertation, Stellenbosch University. Accessed January 19, 2021. http://hdl.handle.net/10019.1/97967.

MLA Handbook (7^th Edition):

Vergotine, Zelda. “Molecular investigation of genetic factors associated with insulin resistance and obesity in a South African population.” 2015. Web. 19 Jan 2021.

Vancouver:

Vergotine Z. Molecular investigation of genetic factors associated with insulin resistance and obesity in a South African population. [Internet] [Doctoral dissertation]. Stellenbosch University; 2015. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10019.1/97967.

Council of Science Editors:

Vergotine Z. Molecular investigation of genetic factors associated with insulin resistance and obesity in a South African population. [Doctoral Dissertation]. Stellenbosch University; 2015. Available from: http://hdl.handle.net/10019.1/97967

University of Hong Kong

13. Bahl, Justin. Molecular evolution of three morphologically similar families in the Xylariomycetidae (Apiosporaceae, Clypeosphaeriaceae, Hyponectriaceae).

Degree: 2006, University of Hong Kong

URL: http://hdl.handle.net/10722/51007

Subjects/Keywords: Ascomycetes - Molecular aspects.; Amphisphaeriaceae - Molecular aspects.; Xylariales - Molecular aspects.

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APA (6^th Edition):

Bahl, J. (2006). Molecular evolution of three morphologically similar families in the Xylariomycetidae (Apiosporaceae, Clypeosphaeriaceae, Hyponectriaceae). (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/51007

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bahl, Justin. “Molecular evolution of three morphologically similar families in the Xylariomycetidae (Apiosporaceae, Clypeosphaeriaceae, Hyponectriaceae).” 2006. Thesis, University of Hong Kong. Accessed January 19, 2021. http://hdl.handle.net/10722/51007.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bahl, Justin. “Molecular evolution of three morphologically similar families in the Xylariomycetidae (Apiosporaceae, Clypeosphaeriaceae, Hyponectriaceae).” 2006. Web. 19 Jan 2021.

Vancouver:

Bahl J. Molecular evolution of three morphologically similar families in the Xylariomycetidae (Apiosporaceae, Clypeosphaeriaceae, Hyponectriaceae). [Internet] [Thesis]. University of Hong Kong; 2006. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10722/51007.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bahl J. Molecular evolution of three morphologically similar families in the Xylariomycetidae (Apiosporaceae, Clypeosphaeriaceae, Hyponectriaceae). [Thesis]. University of Hong Kong; 2006. Available from: http://hdl.handle.net/10722/51007

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Delaware

14. Park, James Hyun-Woo. A single-cell analysis approach to understanding molecular organization and plasticity in the brain.

Degree: PhD, University of Delaware, Department of Chemical and Biomolecular Engineering, 2016, University of Delaware

URL: http://udspace.udel.edu/handle/19716/21464

► Single-cell transcriptional heterogeneity pervades the fully differentiated brain. This heterogeneity is particularly prevalent in brain nuclei involved in the autonomic regulation of physiological functions such… (more)

▼ Single-cell transcriptional heterogeneity pervades the fully differentiated brain. This heterogeneity is particularly prevalent in brain nuclei involved in the autonomic regulation of physiological functions such as cardiovascular homeostasis. Because neuronal function largely depends on its transcriptome, such heterogeneity confounds our understanding of how heterogeneous neurons contribute to their broader phenotypic function. In addition to the transcriptome, functional connectivity and in vivo anatomical environment are additional factors central to defining a neuron’s functional state. Given their importance, these factors may provide the added context necessary to understand how a distribution of heterogeneous neurons contributes to phenotypic function. Consequently, the overall goal of this work is to establish an organizational framework that characterizes single-neuron heterogeneity within a brain nucleus and elucidates its functional relevance. ☐ Towards this goal, we have taken a combined experimental and computational approach to determine the organizing principles driving complex interaction networks within and among transcriptionally diverse neurons within a brain nucleus. First, we generated a large-scale gene expression dataset from several hundred neurons, selected on the basis of their synaptic input types, taken from the nucleus tractus solitarius (NTS), a brainstem nucleus involved in the central regulation of blood pressure. Our analysis of these neurons revealed an organizational structure in which transcriptional variability aligns with synaptic input type along a continuum of graded gene expression. This continuum is populated by distinct neuronal subtypes characterized by gene groups exhibiting correlated expression. ☐ In order to identify the molecular mechanisms driving this correlated behavior, we next developed a fuzzy logic modeling-based methodology to model quantitatively causal gene interaction networks from single-cell transcriptomic data. Our modeling results suggest that distinct input stimuli operating on distinct network structures corresponding to these subtypes can drive neurons through various transcriptional states. These results suggest that transcriptional heterogeneity represents a neuron’s adaptive response to various inputs. Based on these results, we propose that neuronal adaptation may be a mechanism through which the NTS robustly regulates blood pressure and cardiovascular homeostasis. ☐ To test this proposal, we examined what impact adaptation to neuronal subtypes in the NTS and brainstem would have on the short-term autonomic regulation of cardiovascular homeostasis under the simulated disease state of systolic heart failure via mathematical modeling. We developed a closed-loop control model characterizing neuronal regulation of the cardiovascular system by integrating previous quantitative models that simulated various aspects of the cardiovascular system. Because the goal of this study was to investigate the effects of neuronal … Advisors/Committee Members: Ogunnaike, Babatunde A.Schwaber, James S.Vadigepalli, Rajanikanth.

Subjects/Keywords: Brain – Molecular aspects.; Neurons.; Gene expression.; Solitary nucleus – Molecular aspects.; Suprachiasmatic nucleus – Molecular aspects.; Sprague Dawley rats.; Rats as laboratory animals.

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APA (6^th Edition):

Park, J. H. (2016). A single-cell analysis approach to understanding molecular organization and plasticity in the brain. (Doctoral Dissertation). University of Delaware. Retrieved from http://udspace.udel.edu/handle/19716/21464

Chicago Manual of Style (16^th Edition):

Park, James Hyun-Woo. “A single-cell analysis approach to understanding molecular organization and plasticity in the brain.” 2016. Doctoral Dissertation, University of Delaware. Accessed January 19, 2021. http://udspace.udel.edu/handle/19716/21464.

MLA Handbook (7^th Edition):

Park, James Hyun-Woo. “A single-cell analysis approach to understanding molecular organization and plasticity in the brain.” 2016. Web. 19 Jan 2021.

Vancouver:

Park JH. A single-cell analysis approach to understanding molecular organization and plasticity in the brain. [Internet] [Doctoral dissertation]. University of Delaware; 2016. [cited 2021 Jan 19]. Available from: http://udspace.udel.edu/handle/19716/21464.

Council of Science Editors:

Park JH. A single-cell analysis approach to understanding molecular organization and plasticity in the brain. [Doctoral Dissertation]. University of Delaware; 2016. Available from: http://udspace.udel.edu/handle/19716/21464

University of Hong Kong

15. 魏祥. Usefulness of molecular targets for identification of medically important tsukamurella species.

Degree: 2009, University of Hong Kong

URL: http://hdl.handle.net/10722/56700

Subjects/Keywords: Corynebacterium - Molecular aspects.

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APA (6^th Edition):

魏祥.. (2009). Usefulness of molecular targets for identification of medically important tsukamurella species. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/56700

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

魏祥.. “Usefulness of molecular targets for identification of medically important tsukamurella species.” 2009. Thesis, University of Hong Kong. Accessed January 19, 2021. http://hdl.handle.net/10722/56700.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

魏祥.. “Usefulness of molecular targets for identification of medically important tsukamurella species.” 2009. Web. 19 Jan 2021.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

魏祥.. Usefulness of molecular targets for identification of medically important tsukamurella species. [Internet] [Thesis]. University of Hong Kong; 2009. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10722/56700.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

魏祥.. Usefulness of molecular targets for identification of medically important tsukamurella species. [Thesis]. University of Hong Kong; 2009. Available from: http://hdl.handle.net/10722/56700

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

University of Hong Kong

16. 陳信君. Molecular detection of streptococcus sinensis in blood culture samplesfrom hospitalized patients in Hong Kong: aneight-year retrospective study.

Degree: 2011, University of Hong Kong

URL: http://hdl.handle.net/10722/143287

Subjects/Keywords: Streptococcus - Molecular aspects.

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APA (6^th Edition):

陳信君.. (2011). Molecular detection of streptococcus sinensis in blood culture samplesfrom hospitalized patients in Hong Kong: aneight-year retrospective study. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/143287

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

陳信君.. “Molecular detection of streptococcus sinensis in blood culture samplesfrom hospitalized patients in Hong Kong: aneight-year retrospective study.” 2011. Thesis, University of Hong Kong. Accessed January 19, 2021. http://hdl.handle.net/10722/143287.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

陳信君.. “Molecular detection of streptococcus sinensis in blood culture samplesfrom hospitalized patients in Hong Kong: aneight-year retrospective study.” 2011. Web. 19 Jan 2021.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

陳信君.. Molecular detection of streptococcus sinensis in blood culture samplesfrom hospitalized patients in Hong Kong: aneight-year retrospective study. [Internet] [Thesis]. University of Hong Kong; 2011. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10722/143287.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

陳信君.. Molecular detection of streptococcus sinensis in blood culture samplesfrom hospitalized patients in Hong Kong: aneight-year retrospective study. [Thesis]. University of Hong Kong; 2011. Available from: http://hdl.handle.net/10722/143287

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

University of Hong Kong

17. Lin, Ching-nga. Characterization of a novel streptococcus species associated with marine environment.

Degree: 2011, University of Hong Kong

URL: http://hdl.handle.net/10722/144025

Subjects/Keywords: Streptococcus - Molecular aspects.

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APA (6^th Edition):

Lin, C. (2011). Characterization of a novel streptococcus species associated with marine environment. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/144025

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lin, Ching-nga. “Characterization of a novel streptococcus species associated with marine environment.” 2011. Thesis, University of Hong Kong. Accessed January 19, 2021. http://hdl.handle.net/10722/144025.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lin, Ching-nga. “Characterization of a novel streptococcus species associated with marine environment.” 2011. Web. 19 Jan 2021.

Vancouver:

Lin C. Characterization of a novel streptococcus species associated with marine environment. [Internet] [Thesis]. University of Hong Kong; 2011. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10722/144025.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lin C. Characterization of a novel streptococcus species associated with marine environment. [Thesis]. University of Hong Kong; 2011. Available from: http://hdl.handle.net/10722/144025

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University

18. Slauson, Marjorie E. Tumor targeting with a ⁹⁹̳mTcMAG-3 labeled molecular engine.

Degree: MS, Radiation Health Physics, 2006, Oregon State University

URL: http://hdl.handle.net/1957/28773

► A unique tumor targeted method, which may be able to deliver a molecule to the surface of a tumor cell using the pH gradient between… (more)

Subjects/Keywords: Tumors – Molecular aspects

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APA (6^th Edition):

Slauson, M. E. (2006). Tumor targeting with a ⁹⁹̳mTcMAG-3 labeled molecular engine. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/28773

Chicago Manual of Style (16^th Edition):

Slauson, Marjorie E. “Tumor targeting with a ⁹⁹̳mTcMAG-3 labeled molecular engine.” 2006. Masters Thesis, Oregon State University. Accessed January 19, 2021. http://hdl.handle.net/1957/28773.

MLA Handbook (7^th Edition):

Slauson, Marjorie E. “Tumor targeting with a ⁹⁹̳mTcMAG-3 labeled molecular engine.” 2006. Web. 19 Jan 2021.

Vancouver:

Slauson ME. Tumor targeting with a ⁹⁹̳mTcMAG-3 labeled molecular engine. [Internet] [Masters thesis]. Oregon State University; 2006. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1957/28773.

Council of Science Editors:

Slauson ME. Tumor targeting with a ⁹⁹̳mTcMAG-3 labeled molecular engine. [Masters Thesis]. Oregon State University; 2006. Available from: http://hdl.handle.net/1957/28773

Jawaharlal Nehru University

19. Rao, S Chenchal. Molecular aspects of the eye lens and its proteins; -.

Degree: Centre for Cellular and Molecular Biology, 1991, Jawaharlal Nehru University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/32211

Available

References p.86-199

Advisors/Committee Members: Balasubramanlan, D.

Subjects/Keywords: Molecular aspects; proteins

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APA (6^th Edition):

Rao, S. C. (1991). Molecular aspects of the eye lens and its proteins; -. (Thesis). Jawaharlal Nehru University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/32211

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rao, S Chenchal. “Molecular aspects of the eye lens and its proteins; -.” 1991. Thesis, Jawaharlal Nehru University. Accessed January 19, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/32211.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rao, S Chenchal. “Molecular aspects of the eye lens and its proteins; -.” 1991. Web. 19 Jan 2021.

Vancouver:

Rao SC. Molecular aspects of the eye lens and its proteins; -. [Internet] [Thesis]. Jawaharlal Nehru University; 1991. [cited 2021 Jan 19]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/32211.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rao SC. Molecular aspects of the eye lens and its proteins; -. [Thesis]. Jawaharlal Nehru University; 1991. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/32211

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Johannesburg

20. Motsi, Moleboheng Cynthia. Origin and diversification of the Australasian genera Pimelea and Thecanthes (Thymelaeaceae).

Degree: PhD, 2012, University of Johannesburg

URL: http://hdl.handle.net/10210/7121

► Pimelea Banks & Sol. Ex Gaertn. nom. cons. is a large genus consisting of 110 species, of which 90 species are endemic to Australia, 19… (more)

▼ Pimelea Banks & Sol. Ex Gaertn. nom. cons. is a large genus consisting of 110 species, of which 90 species are endemic to Australia, 19 to New Zealand and one to Lord Howe Island. The genus has a great diversity of life forms, breeding systems and habitat. Its closest related genus is Thecanthes Wikstr. Thecanthes comprises five species of annual herbs occurring in the Philippines, New Ireland and northern Australia. Australasian Thecanthes and Pimelea are the only genera within sub-tribe Pimeleinae (angiosperm family Thymelaeaceae) and are characterised by the reduction to two stamens. Here I present the most comprehensive molecular phylogenetic study for Pimelea and Thecanthes. Sequences data from nuclear ITS rDNA and plastid rbcL, rps16, matK and trnL-F intergeneric spacer were used to reconstruct a phylogeny for these genera. I have produced 457 new DNA sequences (five genes and 150 taxa) for the present analyses. The resulting phylogeny was used to assess the taxonomic status of Thecanthes and to evaluate the relationships with Pimeleinae since previous studies indicated a close relationship between Pimelea, Thecanthes and species of Gnidia L. from tropical Africa. The morphological delimitation of sections within Pimelea, the biogeography and the radiation of the genus have been revaluated. Pimelea was found to be monophyletic. It was concluded that Pimelea and Thecanthes are congeneric; consequently a paper has been submitted transferring all species of Thecanthes into Pimelea and making the new combination Pimelea filifolia (Rye) Motsi & Rye. Data analysis revealed very low sequence variation within the subtribe Pimeleinae. This suggested a rapid radiation of the genera, which was confirmed by my molecular dating analyses. Based on molecular clock techniques, I calculated the following ages for the origin of Pimelea: 4.1 mya for New Zealand Pimelea spp. and 13.38 mya for other Pimelea spp. The molecular data also indicated that Pimelea and South Africa Gnidia have a direct common ancestor. I also show that the New Zealand Pimelea are derived and dispersed from Australian

Subjects/Keywords: Thymelaeaceae - Anatomy; Lachnaea - Phylogeny; Philogeny - Molecular aspects

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APA (6^th Edition):

Motsi, M. C. (2012). Origin and diversification of the Australasian genera Pimelea and Thecanthes (Thymelaeaceae). (Doctoral Dissertation). University of Johannesburg. Retrieved from http://hdl.handle.net/10210/7121

Chicago Manual of Style (16^th Edition):

Motsi, Moleboheng Cynthia. “Origin and diversification of the Australasian genera Pimelea and Thecanthes (Thymelaeaceae).” 2012. Doctoral Dissertation, University of Johannesburg. Accessed January 19, 2021. http://hdl.handle.net/10210/7121.

MLA Handbook (7^th Edition):

Motsi, Moleboheng Cynthia. “Origin and diversification of the Australasian genera Pimelea and Thecanthes (Thymelaeaceae).” 2012. Web. 19 Jan 2021.

Vancouver:

Motsi MC. Origin and diversification of the Australasian genera Pimelea and Thecanthes (Thymelaeaceae). [Internet] [Doctoral dissertation]. University of Johannesburg; 2012. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10210/7121.

Council of Science Editors:

Motsi MC. Origin and diversification of the Australasian genera Pimelea and Thecanthes (Thymelaeaceae). [Doctoral Dissertation]. University of Johannesburg; 2012. Available from: http://hdl.handle.net/10210/7121

Columbia University

21. Martin-Carli, Jayne Frances. RPGRIP1L and FTO – genes implicated in the effects of FTO intronic sequence variants on food intake – also affect adipogenesis and adipocyte biology.

Degree: 2017, Columbia University

URL: https://doi.org/10.7916/D8PV6XT2

► Single nucleotides in the first intron of FTO convey effects on adiposity by mechanisms that remain unclear, but appear to include modulation of expression of… (more)

▼ Single nucleotides in the first intron of FTO convey effects on adiposity by mechanisms that remain unclear, but appear to include modulation of expression of FTO itself, as well as other genes (e.g. RPGRIP1L, IRX3) in the vicinity of FTO. This locus affects food intake, the browning of white adipose tissue and risk of type 2 diabetes (independent of its effects on body weight). FTO and RPGRIP1L expression are decreased in fibroblasts and iPSC-derived human neurons of individuals segregating for obesity risk alleles of FTO at rs8050136 and rs1421085. These alleles exhibit decreased binding of isoform p110 of the CUX1 transcription factor. This isoform activates transcription of both FTO and RPGRIP1L. The FTO locus conveys effects on adiposity via hyperphagia, in part, by regulating FTO and RPGRIP1L expression in the hypothalamus. We examined whether FTO and RPGRIP1L also modify adipogenesis and adipose tissue lipid storage. Such effects would influence systemic consequences of the hyperphagia driven by the actions of the genes in the hypothalamus. Given the role in energy homeostasis of genes encoding elements of the primary cilium, we hypothesized that mice hypomorphic for Rpgrip1l would display increased adiposity. In confirmation, we find that Rpgrip1l+/− mice are hyperphagic and obese, and display diminished suppression of food intake in response to leptin administration. These findings suggest that RPGRIP1L may be partly or exclusively responsible for the obesity susceptibility signal at the FTO intronic locus. We describe effects of Rpgrip1l in adipocytes which may contribute to the adiposity phenotype observed in these animals, and possibly humans. Loss of Rpgrip1l in 3T3-L1 preadipocytes increased the number of cells capable of differentiating into mature adipocytes. Knockout of Rpgrip1l in mature adipocytes (using Adipoq-Cre) did not increase adiposity in mice fed chow or high fat diet. Neither did we observe any effects of Rpgrip1l knockdown in mature 3T3-L1 adipocytes in vitro. Thus, to the extent that Rpgrip1l affects cell-autonomous adipose tissue function, it appears to do so by effects conveyed in preadipocytes, a cell type in which the primary cilium – as a mediator of developmental signals – may have functional importance. We propose that decreased RPGRIP1L expression in preadipocytes in humans segregating for FTO-associated obesity risk alleles increases the potential storage capacity of adipose tissue. Such capacity would influence the metabolic consequences of positive energy balance due to the action of these alleles within the brain. Fto expression is upregulated during adipogenesis in murine and human cells in vitro, and is more highly expressed in isolated mouse adipocytes than in preadipocytes. Here we demonstrate that FTO is required for the maintenance of adipocyte lipid filling and endocrine function in murine 3T3-L1 cells and human adipose tissue-derived stromal cells. RNAseq analysis indicates that this effect on adipocyte programming is conveyed in part by modulation…

Subjects/Keywords: Biochemistry; Cytology; Molecular biology; Obesity – Genetic aspects

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APA (6^th Edition):

Martin-Carli, J. F. (2017). RPGRIP1L and FTO – genes implicated in the effects of FTO intronic sequence variants on food intake – also affect adipogenesis and adipocyte biology. (Doctoral Dissertation). Columbia University. Retrieved from https://doi.org/10.7916/D8PV6XT2

Chicago Manual of Style (16^th Edition):

Martin-Carli, Jayne Frances. “RPGRIP1L and FTO – genes implicated in the effects of FTO intronic sequence variants on food intake – also affect adipogenesis and adipocyte biology.” 2017. Doctoral Dissertation, Columbia University. Accessed January 19, 2021. https://doi.org/10.7916/D8PV6XT2.

MLA Handbook (7^th Edition):

Martin-Carli, Jayne Frances. “RPGRIP1L and FTO – genes implicated in the effects of FTO intronic sequence variants on food intake – also affect adipogenesis and adipocyte biology.” 2017. Web. 19 Jan 2021.

Vancouver:

Martin-Carli JF. RPGRIP1L and FTO – genes implicated in the effects of FTO intronic sequence variants on food intake – also affect adipogenesis and adipocyte biology. [Internet] [Doctoral dissertation]. Columbia University; 2017. [cited 2021 Jan 19]. Available from: https://doi.org/10.7916/D8PV6XT2.

Council of Science Editors:

Martin-Carli JF. RPGRIP1L and FTO – genes implicated in the effects of FTO intronic sequence variants on food intake – also affect adipogenesis and adipocyte biology. [Doctoral Dissertation]. Columbia University; 2017. Available from: https://doi.org/10.7916/D8PV6XT2

University of North Carolina – Greensboro

22. Vaidya, Himani. Role of DNA methylation in WNT5A Promoter B expression in Osteosarcoma (SaOS-2) cells.

Degree: 2013, University of North Carolina – Greensboro

URL: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=15663

► WNT5A, a member of the WNT family of secreted proteins, activates the non-canonical WNT pathway, regulates developmental events and is involved in tissue homeostasis. Misregulation… (more)

▼ WNT5A, a member of the WNT family of secreted proteins, activates the non-canonical WNT pathway, regulates developmental events and is involved in tissue homeostasis. Misregulation of WNT5A has been associated with various types of cancer including pancreatic, colorectal, breast, lung and osteosarcoma. Recent studies have shown that WNT5A expression in cancer cells involves non-genetic (epigenetic) changes. The WNT5A gene has two similar transcription start sites (termed promoter A and promoter B in this study) and transcription from these sites give rise to two different messages that encode different protein isoforms. Epigenetic studies to date have focused on the WNT5A promoter A, however our preliminary analysis found that promoter B transcripts are nearly absent in osteosarcoma cells, but present in normal osteoblasts. The goal of this study was to determine if the decrease in promoter B transcripts in osteosarcoma was due to DNA methylation. We identified 6 CpG islands in the WNT5A intron 1 region which contains promoter B and exon 1B, and compared them with data available in NCBI epigenomics website. The NCBI database was also screened for data on the methylation status of these islands in various other cell lines and cancer types. The database analysis shows that there is little or no methylation of Regions 1 - 6 in cells, that normally express WNT5A (fibroblast, chondrocytes, and mesenchymal stem cells). However, in some colorectal tumor tissue there is extensive methylation within intron 1. In adjacent normal colon mucosa tissue, Regions 3 and 4 showed some degree of methylation. Methylation status of the CpG islands in normal osteoblasts and osteosarcoma (SaOS-2) was determined using Sodium bisulfite sequencing. The sequencing data showed that CpG Regions 1 and 2 are unmethylated. Regions 3, 4 and 5 are completely methylated and Region 6 is partially methylated. In normal osteoblasts all 6 regions were unmethylated. A demethylation experiment using 5-Aza-cytidine was performed to determine if decrease in methylation would increase promoter B transcripts. Treatment of SaOS-2 cells with 1µM 5-Aza-cytidine resulted in a 120 fold increase of promoter B transcripts. The methylation data of SaOS-2 cells treated with 1µM 5-Aza-cytidine shows that CpG Region 6 is more prone to demethylation when compared to CpG islands 3, 4, 5. Two clones of Region 6 showed that 4 out of 5 CpG's analyzed were demethylated. 5 clones of Region 3 and 4 showed that only 1-2 CpG's were demethylated out of 24 CpG's in Region 3 and 27 CpG's in Region 4. Overall these results suggest that promoter B transcription is down regulated by DNA methylation of CpG Region 6 and Region 6 is more prone to demethylation compared to Regions 3, 4 and 5.; Epigenetics, Osteosarcoma, WNT5A Advisors/Committee Members: Karen Katula (advisor).

Subjects/Keywords: Wnt proteins; DNA – Methylation; Cancer – Molecular aspects

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vaidya, H. (2013). Role of DNA methylation in WNT5A Promoter B expression in Osteosarcoma (SaOS-2) cells. (Masters Thesis). University of North Carolina – Greensboro. Retrieved from http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=15663

Chicago Manual of Style (16^th Edition):

Vaidya, Himani. “Role of DNA methylation in WNT5A Promoter B expression in Osteosarcoma (SaOS-2) cells.” 2013. Masters Thesis, University of North Carolina – Greensboro. Accessed January 19, 2021. http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=15663.

MLA Handbook (7^th Edition):

Vaidya, Himani. “Role of DNA methylation in WNT5A Promoter B expression in Osteosarcoma (SaOS-2) cells.” 2013. Web. 19 Jan 2021.

Vancouver:

Vaidya H. Role of DNA methylation in WNT5A Promoter B expression in Osteosarcoma (SaOS-2) cells. [Internet] [Masters thesis]. University of North Carolina – Greensboro; 2013. [cited 2021 Jan 19]. Available from: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=15663.

Council of Science Editors:

Vaidya H. Role of DNA methylation in WNT5A Promoter B expression in Osteosarcoma (SaOS-2) cells. [Masters Thesis]. University of North Carolina – Greensboro; 2013. Available from: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=15663

Drexel University

23. Freire-Fierro, Alina. Systematics of Monnina (Polygalaceae).

Degree: 2015, Drexel University

URL: http://hdl.handle.net/1860/idea:7063

►

Endemic to the Neotropics, Monnina is the second largest genus in Polygalaceae, yet little is known about its phylogenetic history and morphological character evolution. Because… (more)

▼

Endemic to the Neotropics, Monnina is the second largest genus in Polygalaceae, yet little is known about its phylogenetic history and morphological character evolution. Because Monnina demonstrates a great deal of morphological variability among species, a phylogenetic study was conducted to resolve where characters important in the diversification of the genus fit within an evolutionary framework. Bayesian and Maximum Likelihood analyses of nuclear ITS and plastid trnL-F regions were used to delimit the placement of Monnina in Polygalaceae and the infrageneric division within the genus. The results show that Monnina is a monophyletic genus subdivided into six clades. Ancestral character reconstructions point towards several synapomorphies in the genus. Indehiscent 1- to 2-seeded fruit is a synapomorphy for Monnina. Clade A is herbaceous, lacks extra-floral nectaries (EFN), and includes a tubular keel apex and 2-seeded dry fruits. The apex of the tubular keel is considered a synapomorphy for Clade A. Although, Clade B is also herbaceous, it lacks EFN in some species, glandular hairs and 2-seeded dry fruits. Glandular hairs are considered the synapomorphy for Clade B. Clade C includes herbaceous, shrub, and arborescent species that lack or exhibit EFN, and includes tri-lobed keels and 1- to 2-seeded fleshy or dry fruits. An irreversible reduction to 1-seeded fruits evolved in the ancestor to Clades D, E and F. While the external members of Clade D have similar characteristics to herbaceous members from Clade C, an internal subclade (D1) is denoted with fire adaptations and a distribution restricted to the Cerrado in southeastern South America. Clade E supports herbaceous plants with stipitate EFN (synapmorphic character). Clade F supports the greatest species range (northern Andes and Central America) and number of species (richness) that are characterized by shrub and arborescent species with fleshy fruits that lack EFN. Further phylogenetic studies that utilize molecular and morphological data will be required to elucidate biogeographical diversification rates.

Ph.D., Environmental Science – Drexel University, 2015

Advisors/Committee Members: Bien, Walter F., College of Arts and Sciences.

Subjects/Keywords: Environmental science; Biogeography; Phylogeny – Molecular aspects

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APA (6^th Edition):

Freire-Fierro, A. (2015). Systematics of Monnina (Polygalaceae). (Thesis). Drexel University. Retrieved from http://hdl.handle.net/1860/idea:7063

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Freire-Fierro, Alina. “Systematics of Monnina (Polygalaceae).” 2015. Thesis, Drexel University. Accessed January 19, 2021. http://hdl.handle.net/1860/idea:7063.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Freire-Fierro, Alina. “Systematics of Monnina (Polygalaceae).” 2015. Web. 19 Jan 2021.

Vancouver:

Freire-Fierro A. Systematics of Monnina (Polygalaceae). [Internet] [Thesis]. Drexel University; 2015. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1860/idea:7063.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Freire-Fierro A. Systematics of Monnina (Polygalaceae). [Thesis]. Drexel University; 2015. Available from: http://hdl.handle.net/1860/idea:7063

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Melbourne

24. Pandian, Anand. Molecular analysis of Vetch (Vicia sativa ssp.sativa cv. Blanche fleur).

Degree: 2002, University of Melbourne

URL: http://hdl.handle.net/11343/114743

Subjects/Keywords: Molecular aspects; Vetch

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pandian, A. (2002). Molecular analysis of Vetch (Vicia sativa ssp.sativa cv. Blanche fleur). (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/114743

Chicago Manual of Style (16^th Edition):

Pandian, Anand. “Molecular analysis of Vetch (Vicia sativa ssp.sativa cv. Blanche fleur).” 2002. Doctoral Dissertation, University of Melbourne. Accessed January 19, 2021. http://hdl.handle.net/11343/114743.

MLA Handbook (7^th Edition):

Pandian, Anand. “Molecular analysis of Vetch (Vicia sativa ssp.sativa cv. Blanche fleur).” 2002. Web. 19 Jan 2021.

Vancouver:

Pandian A. Molecular analysis of Vetch (Vicia sativa ssp.sativa cv. Blanche fleur). [Internet] [Doctoral dissertation]. University of Melbourne; 2002. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/11343/114743.

Council of Science Editors:

Pandian A. Molecular analysis of Vetch (Vicia sativa ssp.sativa cv. Blanche fleur). [Doctoral Dissertation]. University of Melbourne; 2002. Available from: http://hdl.handle.net/11343/114743

University of Hong Kong

25. Sam, Laiju. Molecular biology of a cryptic dehalogenase from Burkholderia cepacia MBA4.

Degree: 2000, University of Hong Kong

URL: http://hdl.handle.net/10722/26199

► (Uncorrected OCR) Abstract of the thesis entitled MOLECULAR BIOLOGY OF A CRYPTIC DEHALOGENASE FROM Burkholderia cepacia MBA4 Submitted by Laiju Sam For the degree of… (more)

▼ (Uncorrected OCR) Abstract of the thesis entitled MOLECULAR BIOLOGY OF A CRYPTIC DEHALOGENASE FROM Burkholderia cepacia MBA4 Submitted by Laiju Sam For the degree of Doctor of Philosophy at The University of Hong Kong in July 2000 Burkholderia cepacia MBA4 has been previously shown to produce a single dehalogenase in batch culture condition. Other cryptic dehalogenases were detected in cells grown in continuous culture. In this study, one of the cryptic dehalogenases was cloned and characterised. This cryptic haloacid dehalogenase was designated Chd 1 and expressed constitutively in Escherichia coli. The structural gene, chdJ, was isolated from a 1.7-kb PstI fragment. This fragment. contains a functional promoter since the expression of chdl in E. coli is orientation independent. The nucleotide sequence of the fragment was determined and analysed and an open reading frame for 840 amino acids was identified. The nucleotide sequence of chdl did not show any homology with those of other dehalogenase genes. Comparison of the deduced amino acid sequence, however, showed significant homology, ranging from 42-50%, with the amino acid sequences of several other dehalogenases. Phylogenetic analysis indicated that Chd 1 is closely related to dehalogenase CI and dehalogenase IVa of Pseudomonas putida CBS3 and B. cepacia MBA4 respectively. 1 The recombinant Chdl had a native molecular weight of 58,000 daltons whereas the denatured molecular weight was found to be 27,000 daltons. Thus, the enzyme exists predominantly as a dimer. When the activity towards the two stereoisomers of 2-monochloropropionic acid were considered individually, the enzyme was found to be active towards the L-isomer only. The purified enzyme was most active towards monobromoacetic acid with specific activities of 5.44, 1.5, 4.56, and 1.95 J!mole of halide released/min/mg protein for monobromoacetic acid, 2-monobromopropionic acid, monochloroacetic acid and 2-monochloropropionic acid respectively. Maximum activity of the enzyme was observed at pH 6.5. The enzyme was found to be thermolabile. chdl was cloned in the T7 RNA polymerase driven pRSET A and Irc promoter based pPROEXHT expression vectors. However, no significant expression of chdl was obtained in these systems. Chd 1 differed from other dehalogenases in having a long leader sequence. This leader sequence contains a potential signal peptidase cleavage site. This is a property of periplasmic enzymes. In order to detect the unprocessed molecules of Chd 1, chdl was expressed in the temperature-sensitive E. coli strain IT 41 (lep9-mutant). This strain contains a mutation in the leader peptidase gene. The leader peptidase is inactive in this strain at the non-permissive temperature of o 42 C. Precursor molecules of Chd 1 was detected in cultures shifted to this non- permissive temperature. Periplasmic fractions isolated from E. coli harboring chdl were found to contain the active dehalogenase. 11

Subjects/Keywords: Pseudomonas - Molecular aspects.

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APA (6^th Edition):

Sam, L. (2000). Molecular biology of a cryptic dehalogenase from Burkholderia cepacia MBA4. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/26199

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sam, Laiju. “Molecular biology of a cryptic dehalogenase from Burkholderia cepacia MBA4.” 2000. Thesis, University of Hong Kong. Accessed January 19, 2021. http://hdl.handle.net/10722/26199.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sam, Laiju. “Molecular biology of a cryptic dehalogenase from Burkholderia cepacia MBA4.” 2000. Web. 19 Jan 2021.

Vancouver:

Sam L. Molecular biology of a cryptic dehalogenase from Burkholderia cepacia MBA4. [Internet] [Thesis]. University of Hong Kong; 2000. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10722/26199.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sam L. Molecular biology of a cryptic dehalogenase from Burkholderia cepacia MBA4. [Thesis]. University of Hong Kong; 2000. Available from: http://hdl.handle.net/10722/26199

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Massey University

26. Moradali, M. Fata. Molecular mechanism of alginate polymerisation and modifications in Pseudomonas aeruginosa : a thesis presented in partial fulfilment of the requirements for degree of Doctor of Philosophy in Microbiology at Massey University, New Zealand .

Degree: 2015, Massey University

URL: http://hdl.handle.net/10179/8286

► Pseudomonas aeruginosa is an ubiquitous opportunistic human pathogen in immunocompromised patients. It is of particular relevance to cystic fibrosis (CF) patients where it frequently causes… (more)

▼ Pseudomonas aeruginosa is an ubiquitous opportunistic human pathogen in immunocompromised patients. It is of particular relevance to cystic fibrosis (CF) patients where it frequently causes chronic bronchopulmonary infection and is the leading cause of morbidity and mortality. The decline in lung function is caused by the emergence of a mucoid variant showing excessive production of the exopolysaccharide, alginate. The alginate-containing biofilm matrix of this mucoid variant protects P. aeruginosa from the immune system and antibiotics. Here the alginate biosynthesis/modification/secretion multiprotein complex was investigated with regard to protein-protein interactions constituting the proposed multiprotein complex and the molecular mechanisms underlying alginate polymerisation and modifications. This study sheds light on the structure and function of various alginates from a material property and biological function perspectives. The binary interactions of AlgK-AlgE, AlgX-Alg44, AlgK-Alg44 and Alg8-Alg44 were identified proposing a new model for this multiprotein complex organisation. Proteinprotein interactions were found to be independent of c-di-GMP binding to PilZAlg44 domain. C-di-GMP-mediated activation of alginate polymerisation was found to be different from activation mechanism proposed for cellulose synthesis. This study showed that alginate polymerisation and modifications were linked. It was shown that the molecular mass of alginate was reduced by epimerisation, while it was increased by acetylation. It was determined that previously characterized proteins AlgG (epimerase) and AlgX (acetyltransferase) have mutual auxiliary and enhancing roles. Biofilm architecture analysis showed that acetyl groups lowered viscoelasticity of alginates and promoted cell aggregation, while nonacetylated polymannuronate alginate promoted stigmergy. Experimental evidence was provided that Alg44 boosted acetylation while the periplasmic domain of this protein was critical for protein stability and regulation of alginate modifications. Full-length Alg44 was purified and it was found to be a dimer in solution. Overall, this study sheds new light on the arrangement of the proposed alginate biosynthesis/modification/secretion multiprotein complex. Furthermore, the activation mechanism and the interplay between polymerisation and modification of alginate were elucidated and new functions and interactive role of alginate-polymerising and– modifying subunits were further understood.

Subjects/Keywords: Pseudomonas aeruginosa; Molecular aspects; Alginates; Polymerization

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APA (6^th Edition):

Moradali, M. F. (2015). Molecular mechanism of alginate polymerisation and modifications in Pseudomonas aeruginosa : a thesis presented in partial fulfilment of the requirements for degree of Doctor of Philosophy in Microbiology at Massey University, New Zealand . (Thesis). Massey University. Retrieved from http://hdl.handle.net/10179/8286

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Moradali, M Fata. “Molecular mechanism of alginate polymerisation and modifications in Pseudomonas aeruginosa : a thesis presented in partial fulfilment of the requirements for degree of Doctor of Philosophy in Microbiology at Massey University, New Zealand .” 2015. Thesis, Massey University. Accessed January 19, 2021. http://hdl.handle.net/10179/8286.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Moradali, M Fata. “Molecular mechanism of alginate polymerisation and modifications in Pseudomonas aeruginosa : a thesis presented in partial fulfilment of the requirements for degree of Doctor of Philosophy in Microbiology at Massey University, New Zealand .” 2015. Web. 19 Jan 2021.

Vancouver:

Moradali MF. Molecular mechanism of alginate polymerisation and modifications in Pseudomonas aeruginosa : a thesis presented in partial fulfilment of the requirements for degree of Doctor of Philosophy in Microbiology at Massey University, New Zealand . [Internet] [Thesis]. Massey University; 2015. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10179/8286.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Moradali MF. Molecular mechanism of alginate polymerisation and modifications in Pseudomonas aeruginosa : a thesis presented in partial fulfilment of the requirements for degree of Doctor of Philosophy in Microbiology at Massey University, New Zealand . [Thesis]. Massey University; 2015. Available from: http://hdl.handle.net/10179/8286

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Stellenbosch University

27. Du Plessis, Kari. The evaluation of the impact of microclimatic factors on grapevine berries in a vineyard setting through molecular profiling.

Degree: PhD, Viticulture and Oenology, 2017, Stellenbosch University

URL: http://hdl.handle.net/10019.1/102890

► ENGLISH SUMMARY: Grape composition is considered to be the result of the grapevine genotype, the environmental factors the grapes are exposed to and the management… (more)

▼ ENGLISH SUMMARY: Grape composition is considered to be the result of the grapevine genotype, the environmental factors the grapes are exposed to and the management practices implemented during their development. However, elucidating how each of these components contributes to the outcome is notoriously difficult under field conditions due to the myriad confounding variables that grapes are influenced by. One of the viticultural management practices frequently implemented in the vineyard is the removal of leaves in the berry bunch zone in order to alter the microclimate of the developing grapes with various potentially advantageous outcomes. However, this common viticultural practice of leaf removal very rarely affects levels of light without elevating bunch temperatures as well. Moreover, definitive links between this treatment and the underlying grape molecular responses are currently lacking, particularly in cause and effect relationships. Utilizing a highly characterized Sauvignon Blanc vineyard, a leaf removal treatment was implemented according to a field-omics experimental approach, in which it was established that light exposure to the developing grapes was the predominant factor modulated by the treatment. A preceding study characterized the physical growth parameters of the developing grapes and targeted specific metabolites in order to determine how elevated light would affect grape development. The results revealed that the growth and development of the grapes were not affected by the treatment, but that specific secondary metabolites with photoprotective abilities were elevated. These results showed that the grapes acclimated to the elevated light exposure, providing the possibility to study the molecular mechanisms associated with this acclimated state in the berries. The aim of this study was therefore to explore the transcriptional responses of the developing grape berries to elevated light exposure to understand how primary metabolism and growth was maintained despite the implementation of stress mitigation strategies. The approach taken to study this transcriptional response involved RNA sequencing (RNASeq) analysis in order to generate a transcriptional snapshot of all the genes expressed in control and light exposed grapes sampled at four developmental stages throughout berry development. This analysis revealed that the green grapes implemented several photoprotective mechanisms simultaneously. Some of these mechanisms involved non-photochemical quenching and the rapid turnover of the proteins of the photosynthetic machinery, much like other foliar photosynthetic tissues, despite the profound differences in photosynthesis dynamics between these tissue types. Additionally, the genes associated with the synthesis of flavonoid compounds were significantly upregulated and these findings were further corroborated by the accumulation of high levels of flavonols that are known to have both light absorbing and antioxidant abilities. In combination, through these … Advisors/Committee Members: Vivier, Melane Alethea, Young, Philip R., Stellenbosch University. Faculty of AgriSciences. Dept. of Viticulture and Oenology..

Subjects/Keywords: Viticulture – Environmental aspects; Microclimatology; Grapes – Climatic factors; Molecular mechanisms; Photosynthesis – Molecular aspects; Biochemical markers; UCTD

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APA (6^th Edition):

Du Plessis, K. (2017). The evaluation of the impact of microclimatic factors on grapevine berries in a vineyard setting through molecular profiling. (Doctoral Dissertation). Stellenbosch University. Retrieved from http://hdl.handle.net/10019.1/102890

Chicago Manual of Style (16^th Edition):

Du Plessis, Kari. “The evaluation of the impact of microclimatic factors on grapevine berries in a vineyard setting through molecular profiling.” 2017. Doctoral Dissertation, Stellenbosch University. Accessed January 19, 2021. http://hdl.handle.net/10019.1/102890.

MLA Handbook (7^th Edition):

Du Plessis, Kari. “The evaluation of the impact of microclimatic factors on grapevine berries in a vineyard setting through molecular profiling.” 2017. Web. 19 Jan 2021.

Vancouver:

Du Plessis K. The evaluation of the impact of microclimatic factors on grapevine berries in a vineyard setting through molecular profiling. [Internet] [Doctoral dissertation]. Stellenbosch University; 2017. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10019.1/102890.

Council of Science Editors:

Du Plessis K. The evaluation of the impact of microclimatic factors on grapevine berries in a vineyard setting through molecular profiling. [Doctoral Dissertation]. Stellenbosch University; 2017. Available from: http://hdl.handle.net/10019.1/102890

University of Delaware

28. Main, Christopher Robin. Molecular assessment of raphidophyte species and their association with Vibrio in Delaware's inland bays.

Degree: PhD, University of Delaware, School of Marine Science & Policy, 2014, University of Delaware

URL: http://udspace.udel.edu/handle/19716/16814

► There is little information on the dynamics between bacteria and phytoplankton within the marine environment. One genus of bacteria, Vibrio is often found in association… (more)

▼ There is little information on the dynamics between bacteria and phytoplankton within the marine environment. One genus of bacteria, Vibrio is often found in association with eukaryotic organisms, forming relationships that range from mutualistic to pathogenic in nature. In this dissertation, I investigated community-level and species-specific associations between Vibrio and three algal classes (diatoms, dinoflagellates, and raphidophytes) that form frequent blooms in Delaware's inland bays. Results demonstrated a significant correlation between particle-associated Vibrio abundance and all three algal classes. Further species-specific interactions were examined over the course of four days during a mixed bloom of the harmful algal bloom species (HABs) Heterosigma akashiwo (3.0-20 μm size fraction) and Fibrocapsa japonica (>20 μm size fraction). Results indicated a closer relationship for particle-associated Vibrio abundance with H. akashiwo abundance. Changes in the Vibrio community during the bloom were examined using automated ribosomal intergenic spacer analysis (ARISA) and showed differences between size fractions but not over time. Grazing experiments during a separate mixed raphidophyte bloom demonstrated that associations with different size fractions may provide refuge for certain members of the Vibrio community despite an increased grazing pressure on the total population. Species-specific interactions were then examined showing that V. cholerae was the major species associated with H. akashiwo . The transcriptional response of H. akashiwo to the presence of V. cholerae was evaluated using next generation sequencing, representing the first reported transcriptome of a raphidophyte species. This transcriptome was then used to examine differential expression of genes from H. akashiwo in response to the presence of V. cholerae . Genes related to antioxidant scavenging, amino acid transport, post-translation modification and translation were significantly up regulated, while those associated with energy production and conversion, and lipid transport and metabolism were down regulated. Finally, direct comparison of quantitative real-time PCR (qPCR) and sandwich hybridization assay (SHA) was carried out using H. akashiwo as a model organism. The effects of diel cycle, growth stage, and macronutrient stress on enumeration of H. akashiwo was examined on both methods. A singular cellular homogenate was generated from each culture and split for analysis by qPCR and SHA. Results showed significantly greater rRNA content during lag and exponential phases compared to stationary phase for SHA, and a significant decrease in rRNA during the light cycle compared to the dark cycle. In contrast, there were no significant differences for qPCR over a diel cycle or during the different growth stages. However, under N stress both rRNA and DNA content per cell were significantly lower when compared to nutrient replete conditions. This work increases our understanding of dynamics between harmful algal species and Vibrio spp. in… Advisors/Committee Members: Coyne, Kathryn J..

Subjects/Keywords: Vibrio – Delaware – Molecular aspects.; Raphidophyceae – Delaware – Molecular aspects.; Diatoms – Delaware.; Dinoflagellates – Delaware.; Heterosigma akashiwo – Delaware.

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APA (6^th Edition):

Main, C. R. (2014). Molecular assessment of raphidophyte species and their association with Vibrio in Delaware's inland bays. (Doctoral Dissertation). University of Delaware. Retrieved from http://udspace.udel.edu/handle/19716/16814

Chicago Manual of Style (16^th Edition):

Main, Christopher Robin. “Molecular assessment of raphidophyte species and their association with Vibrio in Delaware's inland bays.” 2014. Doctoral Dissertation, University of Delaware. Accessed January 19, 2021. http://udspace.udel.edu/handle/19716/16814.

MLA Handbook (7^th Edition):

Main, Christopher Robin. “Molecular assessment of raphidophyte species and their association with Vibrio in Delaware's inland bays.” 2014. Web. 19 Jan 2021.

Vancouver:

Main CR. Molecular assessment of raphidophyte species and their association with Vibrio in Delaware's inland bays. [Internet] [Doctoral dissertation]. University of Delaware; 2014. [cited 2021 Jan 19]. Available from: http://udspace.udel.edu/handle/19716/16814.

Council of Science Editors:

Main CR. Molecular assessment of raphidophyte species and their association with Vibrio in Delaware's inland bays. [Doctoral Dissertation]. University of Delaware; 2014. Available from: http://udspace.udel.edu/handle/19716/16814

Brock University

29. Boyle, Patrick. Unique and unifying themes in the mechanisms regulating the expression of the arabidopsis thaliana PR-1 and Solanum tuberosum PR-10a inducible defense genes .

Degree: Centre for Biotechnology, 2010, Brock University

URL: http://hdl.handle.net/10464/3031

► Arabidopsis is a model plant used to study disease resistance; Solanum tuberosum or potato is a crop species. Both plants possess inducible defense mechanisms that… (more)

▼ Arabidopsis is a model plant used to study disease resistance; Solanum tuberosum or potato is a crop species. Both plants possess inducible defense mechanisms that are deployed upon recognition of pathogen invasion. Transcriptional reprogramming is crucial to the activation of defense responses. The Pathogenesis-Related (PR) genes are activated in these defense programs. Expression of Arabidopsis PR-l and potato PR-10a serve as markers for the deployment of defense responses in these plants. PR-l expression indicates induction of systemic acquired resistance (SAR). Activation of SAR requires accumulation of salicylic acid (SA), in addition to the interaction of the non-expressor of pathogenesis-related genes I (NPRI), with the TGA transcription factors. The PR-10a is activated in response to pathogen invasion, wounding and elicitor treatment. PR-10a induction requires recruitment of the Whirly I (Whyl) activator to the promoter. This locus is also negatively regulated by the silencer element binding factor (SEBF). We established that both the PR-l and PR-10a are occupied by repressors under non-inducing conditions. TGA2 was found to be a constitutive resident and repressor of PR-l, which mediates repression by forming an oligomeric complex on the promoter. The DNA-binding activity of this oligomer required the TGA2 N-terminus (NT). Under resting conditions we determined that the PR-10a is bound by a repressosome containing SEBF and curiously the activator Pto interacting protein 4 (Pti4). In the context of this repressosome, SEBF is responsible for PR-10a binding, yet rWe also showed that PR-l and PR-10a are activated by different means. In PR-l activation the NPRI NT domain alleviates TGA2-mediated repression by interacting with the TGA2 NT. TGA2 remains at the PR-l but adopts a dimeric conformation and forms an enhanceosome with NPRl. In contrast, the PR-10a is activated by evicting the repressosome and recruiting Why! to the promoter. These results advance our understanding of the mechanisms regulating PR-l and PR-10a expression under resting and inducing conditions. This study also revealed that the means of regulation for related genes can differ greatly between model and crop s

Subjects/Keywords: Arabidopsis – Molecular aspects; Solanum – Molecular aspects

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APA (6^th Edition):

Boyle, P. (2010). Unique and unifying themes in the mechanisms regulating the expression of the arabidopsis thaliana PR-1 and Solanum tuberosum PR-10a inducible defense genes . (Thesis). Brock University. Retrieved from http://hdl.handle.net/10464/3031

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Boyle, Patrick. “Unique and unifying themes in the mechanisms regulating the expression of the arabidopsis thaliana PR-1 and Solanum tuberosum PR-10a inducible defense genes .” 2010. Thesis, Brock University. Accessed January 19, 2021. http://hdl.handle.net/10464/3031.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Boyle, Patrick. “Unique and unifying themes in the mechanisms regulating the expression of the arabidopsis thaliana PR-1 and Solanum tuberosum PR-10a inducible defense genes .” 2010. Web. 19 Jan 2021.

Vancouver:

Boyle P. Unique and unifying themes in the mechanisms regulating the expression of the arabidopsis thaliana PR-1 and Solanum tuberosum PR-10a inducible defense genes . [Internet] [Thesis]. Brock University; 2010. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10464/3031.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Boyle P. Unique and unifying themes in the mechanisms regulating the expression of the arabidopsis thaliana PR-1 and Solanum tuberosum PR-10a inducible defense genes . [Thesis]. Brock University; 2010. Available from: http://hdl.handle.net/10464/3031

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Hong Kong

30. 武廣文. Molecular simulation studies of platinum and oxygen on a graphite surface.

Degree: 1997, University of Hong Kong

URL: http://hdl.handle.net/10722/35100

Subjects/Keywords: Oxygen - Molecular aspects.; Platinum - Molecular aspects.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

武廣文. (1997). Molecular simulation studies of platinum and oxygen on a graphite surface. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/35100

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

武廣文. “Molecular simulation studies of platinum and oxygen on a graphite surface.” 1997. Thesis, University of Hong Kong. Accessed January 19, 2021. http://hdl.handle.net/10722/35100.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

武廣文. “Molecular simulation studies of platinum and oxygen on a graphite surface.” 1997. Web. 19 Jan 2021.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

武廣文. Molecular simulation studies of platinum and oxygen on a graphite surface. [Internet] [Thesis]. University of Hong Kong; 1997. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10722/35100.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

武廣文. Molecular simulation studies of platinum and oxygen on a graphite surface. [Thesis]. University of Hong Kong; 1997. Available from: http://hdl.handle.net/10722/35100

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

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