You searched for subject:( bile acid).
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Indian Institute of Science
1.
Satyanarayana, T B N.
Design And Synthesis Of Bile Acid Derived Oligomers And Study Of Their Aggregation And Potential Applications.
Degree: PhD, Faculty of Science, 2015, Indian Institute of Science
URL: http://etd.iisc.ac.in/handle/2005/2429 
► Chapter 1: Amphiphilic self-assembled systems as nanocarriers Nanocarriers are the nanometric size molecular assemblies that are used for the transport of small molecules into their…
(more)
▼ Chapter 1: Amphiphilic self-assembled systems as nanocarriers
Nanocarriers are the nanometric size molecular assemblies that are used for the transport of small molecules into their non-solvating environments. These systems find major applications as drug delivery systems (DDS) in pharmacological research. These drug delivery systems improves solubility and stability of the drug molecules through encapsulation and also offer additional advantages like target specificity and stimuli responsive release of the drug molecules. Several types of DDS are reported in the literature, which can be prepared by a variety of processing techniques. Of these, molecular self-
Chart 1: Developments in the design of amphiphilic nanocarriers
assembly has attained considerable attention due to its greater tunability and control in the preparation of nanocarriers. In this chapter we discussed about the amphiphilic nanocarriers which are prepared through self-assembly of amphiphiles through hydrophobic interactions. Several developments in the area of amphiphilic nanocarriers such as di-block polymeric systems, dendritic systems and core-shell architectures are also mentioned. We also highlighted some recent developments in the design of amphiphilic nanocarriers through supramolecular interactions and advantages of such systems.
Chapter 2:
Bile acid derived dendrons and their application as nanocarriers
Host-guest chemistry is well known for dendritic systems. To understand the influence of steric crowding, dendritic effect and importance of number of hydroxyl groups on the
bile acid backbone in the host-guest chemistry of
bile acid dendrons, we designed and synthesized a new series of C3 symmetric systems and studied the above-mentioned objectives through extraction of polar dyes into nonpolar media. Dye extraction experiments performed using trimeric molecules suggested that only the cholate derivatives (3 and 4) showed considerable extraction of the polar dyes into chloroform; deoxycholate derivatives did not show any extraction, thus emphasizing the importance of the number of hydroxyl groups for dye extraction in these molecular architectures. The effect of steric crowding at the core of these trimeric molecules was shown by efficient extraction of the dyes with the triethylbenzene core (4) compared to the benzene core (3). Greater influence of the aggregates in the case of triethylbenzene core on the extracted dye was also manifested in the
Chart 2: Structures of the designed molecules 1-6
value of the induced circular dichroism signal. Surprisingly, a higher analogue in these molecular architectures showed lesser efficiency in dye extraction (on a per
bile acid residue basis) compared to the trimers, suggesting a more compact structure for the higher analogue. This was supported by molecular modeling studies. Generality of these systems as nanocarriers for hydrophilic dyes was investigated by screening several other dyes and polar molecules, which are diverse in their structure and functionalities. All these experiments…
Advisors/Committee Members: Maitra, Uday (advisor).
Subjects/Keywords: Bile Acids; Oligomers; Drug Delivery System; Amphiphilic Nanocarriers; Bile Acid Dendrons; Oligomeric Bile Acid-taurine Conjugates; Bile Acid Oligomers - Synthesis; Perylene-bile Acid Conjugates; Dendrimers; Dendrons; Organic Chemistry
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APA (6th Edition):
Satyanarayana, T. B. N. (2015). Design And Synthesis Of Bile Acid Derived Oligomers And Study Of Their Aggregation And Potential Applications. (Doctoral Dissertation). Indian Institute of Science. Retrieved from http://etd.iisc.ac.in/handle/2005/2429
Chicago Manual of Style (16th Edition):
Satyanarayana, T B N. “Design And Synthesis Of Bile Acid Derived Oligomers And Study Of Their Aggregation And Potential Applications.” 2015. Doctoral Dissertation, Indian Institute of Science. Accessed March 08, 2021.
http://etd.iisc.ac.in/handle/2005/2429.
MLA Handbook (7th Edition):
Satyanarayana, T B N. “Design And Synthesis Of Bile Acid Derived Oligomers And Study Of Their Aggregation And Potential Applications.” 2015. Web. 08 Mar 2021.
Vancouver:
Satyanarayana TBN. Design And Synthesis Of Bile Acid Derived Oligomers And Study Of Their Aggregation And Potential Applications. [Internet] [Doctoral dissertation]. Indian Institute of Science; 2015. [cited 2021 Mar 08].
Available from: http://etd.iisc.ac.in/handle/2005/2429.
Council of Science Editors:
Satyanarayana TBN. Design And Synthesis Of Bile Acid Derived Oligomers And Study Of Their Aggregation And Potential Applications. [Doctoral Dissertation]. Indian Institute of Science; 2015. Available from: http://etd.iisc.ac.in/handle/2005/2429
Delft University of Technology
2.
Kwekkeboom, J.
Regulation of bile acid synthesis in cultured hepatocytes.
Degree: 1990, Delft University of Technology
URL: http://resolver.tudelft.nl/uuid:2107e7fc-45b1-4f7f-a735-6ad7d2529fd8
;
urn:NBN:nl:ui:24-uuid:2107e7fc-45b1-4f7f-a735-6ad7d2529fd8
;
urn:NBN:nl:ui:24-uuid:2107e7fc-45b1-4f7f-a735-6ad7d2529fd8
;
http://resolver.tudelft.nl/uuid:2107e7fc-45b1-4f7f-a735-6ad7d2529fd8
Subjects/Keywords: Biology; Bile acid
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APA (6th Edition):
Kwekkeboom, J. (1990). Regulation of bile acid synthesis in cultured hepatocytes. (Doctoral Dissertation). Delft University of Technology. Retrieved from http://resolver.tudelft.nl/uuid:2107e7fc-45b1-4f7f-a735-6ad7d2529fd8 ; urn:NBN:nl:ui:24-uuid:2107e7fc-45b1-4f7f-a735-6ad7d2529fd8 ; urn:NBN:nl:ui:24-uuid:2107e7fc-45b1-4f7f-a735-6ad7d2529fd8 ; http://resolver.tudelft.nl/uuid:2107e7fc-45b1-4f7f-a735-6ad7d2529fd8
Chicago Manual of Style (16th Edition):
Kwekkeboom, J. “Regulation of bile acid synthesis in cultured hepatocytes.” 1990. Doctoral Dissertation, Delft University of Technology. Accessed March 08, 2021.
http://resolver.tudelft.nl/uuid:2107e7fc-45b1-4f7f-a735-6ad7d2529fd8 ; urn:NBN:nl:ui:24-uuid:2107e7fc-45b1-4f7f-a735-6ad7d2529fd8 ; urn:NBN:nl:ui:24-uuid:2107e7fc-45b1-4f7f-a735-6ad7d2529fd8 ; http://resolver.tudelft.nl/uuid:2107e7fc-45b1-4f7f-a735-6ad7d2529fd8.
MLA Handbook (7th Edition):
Kwekkeboom, J. “Regulation of bile acid synthesis in cultured hepatocytes.” 1990. Web. 08 Mar 2021.
Vancouver:
Kwekkeboom J. Regulation of bile acid synthesis in cultured hepatocytes. [Internet] [Doctoral dissertation]. Delft University of Technology; 1990. [cited 2021 Mar 08].
Available from: http://resolver.tudelft.nl/uuid:2107e7fc-45b1-4f7f-a735-6ad7d2529fd8 ; urn:NBN:nl:ui:24-uuid:2107e7fc-45b1-4f7f-a735-6ad7d2529fd8 ; urn:NBN:nl:ui:24-uuid:2107e7fc-45b1-4f7f-a735-6ad7d2529fd8 ; http://resolver.tudelft.nl/uuid:2107e7fc-45b1-4f7f-a735-6ad7d2529fd8.
Council of Science Editors:
Kwekkeboom J. Regulation of bile acid synthesis in cultured hepatocytes. [Doctoral Dissertation]. Delft University of Technology; 1990. Available from: http://resolver.tudelft.nl/uuid:2107e7fc-45b1-4f7f-a735-6ad7d2529fd8 ; urn:NBN:nl:ui:24-uuid:2107e7fc-45b1-4f7f-a735-6ad7d2529fd8 ; urn:NBN:nl:ui:24-uuid:2107e7fc-45b1-4f7f-a735-6ad7d2529fd8 ; http://resolver.tudelft.nl/uuid:2107e7fc-45b1-4f7f-a735-6ad7d2529fd8
Texas A&M University
3.
Yang, Isabelle Fan.
Understanding the Relationship of Bile Acid Binding Capacity, Phenolic Compounds and Their Bioaccessibility of Selected Vegetables.
Degree: MS, Food Science and Technology, 2017, Texas A&M University
URL: http://hdl.handle.net/1969.1/166105
► Vegetables with bile acid capacity provide multiple benefits to human health, including reducing plasma cholesterol levels, controlling blood sugar in type 2 diabetic patients, and…
(more)
▼ Vegetables with
bile acid capacity provide multiple benefits to human health, including reducing plasma cholesterol levels, controlling blood sugar in type 2 diabetic patients, and preventing colon cancer. The in vitro
bile acid binding capacities of Brussels sprouts, green kale, red kale, red cabbage, and red leaf lettuce were tested and their dietary fiber content was analyzed. Green kale was used for further studies to explore the optimal dose for
bile acid binding, the effect of
bile acid composition on the binding capacity, the influence of minimal processing on the
bile acid binding capacity, the interaction of bioactive compounds with
bile acids, and the impact of
bile acids on the bioaccessibility of kale polyphenols.
The in vitro digestion was conducted in three phases that simulated oral, gastric, and intestinal digestion.
Bile acids were incubated with vegetables in the intestinal digestion phase. Kale had the greater
bile acid binding capacity compared with Brussels sprouts, red cabbage, and red leaf lettuce. In the experiment testing the effect of different
bile acid compositions on
bile acid binding capacity, kale showed a similar binding capacity for the
bile acid composition simulating that found in healthy females and males with gallstones, but it bound less
bile acids when the composition simulated the
bile acid pool of type-2 diabetic males. The type-2 diabetic male patient model was used to explore
bile acid binding capacity in response to different doses of kale. The results suggested that the optimal dose of kale was 1.8 g, which bound 81.8% of the added
bile acid. Microwaving and steaming significantly improved kale’s in vitro
bile acid binding capacity.
To study the interaction between
bile acids and different bioactive compounds in kale, polyphenols were separated from the fiber-rich kale tissue, and both of these were incubated with
bile acids. We found that the fiber-rich tissue in kale was the main component that binds
bile acids. Similar in vitro digestions both with and without
bile acids suggested that
bile acids improved the bioaccessibility of quercetin and the total identified polyphenols in kale. Therefore,
bile acids can be bound by fiber rich tissues in the kale and have some interactions with kale polyphenols.
Advisors/Committee Members: Patil, Bhimu (advisor), Turner, Nancy D (committee member), Jayaprakasha, G K (committee member), Awika, Joseph M (committee member).
Subjects/Keywords: bile acid; polyphenols; phenolic compounds; bioaccessibility; in vitro digestion; bile salts; in vitro bile acid binding
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APA (6th Edition):
Yang, I. F. (2017). Understanding the Relationship of Bile Acid Binding Capacity, Phenolic Compounds and Their Bioaccessibility of Selected Vegetables. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/166105
Chicago Manual of Style (16th Edition):
Yang, Isabelle Fan. “Understanding the Relationship of Bile Acid Binding Capacity, Phenolic Compounds and Their Bioaccessibility of Selected Vegetables.” 2017. Masters Thesis, Texas A&M University. Accessed March 08, 2021.
http://hdl.handle.net/1969.1/166105.
MLA Handbook (7th Edition):
Yang, Isabelle Fan. “Understanding the Relationship of Bile Acid Binding Capacity, Phenolic Compounds and Their Bioaccessibility of Selected Vegetables.” 2017. Web. 08 Mar 2021.
Vancouver:
Yang IF. Understanding the Relationship of Bile Acid Binding Capacity, Phenolic Compounds and Their Bioaccessibility of Selected Vegetables. [Internet] [Masters thesis]. Texas A&M University; 2017. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/1969.1/166105.
Council of Science Editors:
Yang IF. Understanding the Relationship of Bile Acid Binding Capacity, Phenolic Compounds and Their Bioaccessibility of Selected Vegetables. [Masters Thesis]. Texas A&M University; 2017. Available from: http://hdl.handle.net/1969.1/166105
Bucknell University
4.
Taylor, Brandy Nicole.
Studying Electrophoretic Separations Using Cholate Micelles: Effects Of Ph, Temperature, And Composition.
Degree: 2013, Bucknell University
URL: https://digitalcommons.bucknell.edu/masters_theses/109
► Micelle-forming bile salts have previously been shown to be effective pseudo-stationary phases for separating the chiral isomers of binaphthyl compounds with micellar electrokinetic capillary chromatography…
(more)
▼ Micelle-forming bile salts have previously been shown to be effective pseudo-stationary phases for separating the chiral isomers of binaphthyl compounds with micellar electrokinetic capillary chromatography (MEKC). Here, cholate micelles are systematically investigated via electrophoretic separations and NMR using R, S-1, 1¿- binaphthyl- 2, 2¿-diylhydrogenphosphate (BNDHP) as a model chiral analyte. The pH, temperature, and concentration of BNDHP were systematically varied while monitoring the chiral resolution obtained with MEKC and the chemical shift of various protons in NMR. NMR data for each proton on BNDHP is monitored as a function of cholate concentration: as cholate monomers begin to aggregate and the analyte molecules begin to sample the micelle aggregate we observe changes in the cholate methyl and S-BNDHP proton chemical shifts. From such NMR data, the apparent CMC of cholate at pH 12 is found to be about 13-14 mM, but this value decreases at higher pH, suggesting that more extreme pHs may give rise to more effective separations. In general, CMCs increase with temperature indicating that one may be able to obtain better separations at lower temperatures. S-BNDHP concentrations ranging from 50 ¿M to 400 ¿M (pH 12.8) gave rise to apparent cholate CMC values from 10 mM to 8 mM, respectively, indicating that S-BNDHP, the chiral analyte molecule, may play an active role in stabilizing cholate aggregates. In all, these data show that NMR can be used to systematically investigate a complex multi-variable landscape of potential optimizations of chiral separations.
Subjects/Keywords: NMR; Micelle; cholate; electrophoretic separations; MEKC; capillary electrophoresis; bile salt; bile acid; BNDHP
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APA (6th Edition):
Taylor, B. N. (2013). Studying Electrophoretic Separations Using Cholate Micelles: Effects Of Ph, Temperature, And Composition. (Thesis). Bucknell University. Retrieved from https://digitalcommons.bucknell.edu/masters_theses/109
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Taylor, Brandy Nicole. “Studying Electrophoretic Separations Using Cholate Micelles: Effects Of Ph, Temperature, And Composition.” 2013. Thesis, Bucknell University. Accessed March 08, 2021.
https://digitalcommons.bucknell.edu/masters_theses/109.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Taylor, Brandy Nicole. “Studying Electrophoretic Separations Using Cholate Micelles: Effects Of Ph, Temperature, And Composition.” 2013. Web. 08 Mar 2021.
Vancouver:
Taylor BN. Studying Electrophoretic Separations Using Cholate Micelles: Effects Of Ph, Temperature, And Composition. [Internet] [Thesis]. Bucknell University; 2013. [cited 2021 Mar 08].
Available from: https://digitalcommons.bucknell.edu/masters_theses/109.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Taylor BN. Studying Electrophoretic Separations Using Cholate Micelles: Effects Of Ph, Temperature, And Composition. [Thesis]. Bucknell University; 2013. Available from: https://digitalcommons.bucknell.edu/masters_theses/109
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Toledo Health Science Campus
5.
Almarghalani, Daniyah Abduljalil.
Molecular Cloning, Expression, and Characterization of A
Novel ZebrafishCytosolic Sulfotransferase, SULT5A1.
Degree: MSP, Pharmaceutical Sciences
(Pharmacology/Toxicology), 2016, University of Toledo Health Science Campus
URL: http://rave.ohiolink.edu/etdc/view?acc_num=mco1470097207
► Sulfotransferase enzymes (SULTs) are responsible for phase II detoxification of xenobiotics as well as regulation of many endogenous compounds, including thyroid/ steroid hormones, bile acids,…
(more)
▼ Sulfotransferase enzymes (SULTs) are responsible for
phase II detoxification of xenobiotics as well as regulation of
many endogenous compounds, including thyroid/ steroid hormones,
bile acids, and catecholamine neurotransmitters. In recent years,
zebrafish is emerging as an important animal model for drug
metabolism research. This study is part of an overall effort to
establish the zebrafish as a model for studying drug sulfation. By
searching the GenBank database, the last remaining zebrafish
sequence encoding a putative SULT, designated SULT5A1, was
identified. Zebrafish SULT5A1 was subsequently cloned, expressed,
purified, and characterized. Substrate specificity of zebrafish
SULT5A1 was analyzed using a panel of more than 147 xenobiotics,
endogenous compound,
bile acids, and commercially available
bile
alcohols. SULT5A1 showed strong sulfating activity toward
bile acid
and
bile alcohol compounds, including 5a-cyprinol, 5ß-cyprinol, 5ß-
scymnol, 5ß-cholestantriol, PZ, and 5a-lithocholic
acid. It also
exhibited significant activity toward endogenous compound,
including DHEA and pregnenolone. However, SULT5A1 showed no
activity toward xenobiotics. pH dependence and kinetic studies were
performed using zebrafish SULT5A1 with 5a-cyprinol, 5a-petromyzonol
(PZ), DHEA, and pregnenolone as substrates.
Advisors/Committee Members: Liu, Ming-Cheh (Committee Chair).
Subjects/Keywords: Pharmacology; Pharmacy Sciences; Pharmaceuticals; Sulfotransferase; SULT; Sulfation; Zebrafish; Bile acid; Bile alcohol; DHEA, Pregnenolone
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APA ·
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APA (6th Edition):
Almarghalani, D. A. (2016). Molecular Cloning, Expression, and Characterization of A
Novel ZebrafishCytosolic Sulfotransferase, SULT5A1. (Masters Thesis). University of Toledo Health Science Campus. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=mco1470097207
Chicago Manual of Style (16th Edition):
Almarghalani, Daniyah Abduljalil. “Molecular Cloning, Expression, and Characterization of A
Novel ZebrafishCytosolic Sulfotransferase, SULT5A1.” 2016. Masters Thesis, University of Toledo Health Science Campus. Accessed March 08, 2021.
http://rave.ohiolink.edu/etdc/view?acc_num=mco1470097207.
MLA Handbook (7th Edition):
Almarghalani, Daniyah Abduljalil. “Molecular Cloning, Expression, and Characterization of A
Novel ZebrafishCytosolic Sulfotransferase, SULT5A1.” 2016. Web. 08 Mar 2021.
Vancouver:
Almarghalani DA. Molecular Cloning, Expression, and Characterization of A
Novel ZebrafishCytosolic Sulfotransferase, SULT5A1. [Internet] [Masters thesis]. University of Toledo Health Science Campus; 2016. [cited 2021 Mar 08].
Available from: http://rave.ohiolink.edu/etdc/view?acc_num=mco1470097207.
Council of Science Editors:
Almarghalani DA. Molecular Cloning, Expression, and Characterization of A
Novel ZebrafishCytosolic Sulfotransferase, SULT5A1. [Masters Thesis]. University of Toledo Health Science Campus; 2016. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=mco1470097207
Texas A&M University
6.
Guard, Blake Crosby.
Microbial Characterization, Metabolomic Profiling, and Bile Acid Metabolism in Healthy Dogs and Dogs with Chronic Enteropathy.
Degree: PhD, Biomedical Sciences, 2017, Texas A&M University
URL: http://hdl.handle.net/1969.1/165797
► Chronic gastrointestinal disease in dogs can manifest itself in many different ways including vomiting, diarrhea, and weight loss. Bile acid dysmetabolism has recently been recognized…
(more)
▼ Chronic gastrointestinal disease in dogs can manifest itself in many different ways including vomiting, diarrhea, and weight loss.
Bile acid dysmetabolism has recently been recognized as an important component of chronic gastrointestinal disease (e.g., Crohn’s disease, Ulcerative Colitis, Irritable Bowel Syndrome, and Inflammatory Bowel Disease) in humans. The aim of this research was to evaluate
bile acid dysmetabolism in chronic enteropathy of dogs. An assay for the measurement of unconjugated fecal
bile acids using gas chromatography coupled with mass spectrometry was developed.
The assay was accurate and reproducible. The percent of unconjugated secondary
bile acids were significantly decreased in dogs with chronic enteropathy (p=0.0161), with approximately 60% of dogs having
bile acid dysmetabolism. The percent of unconjugated secondary
bile acids significantly increased in patients with chronic enteropathy after steroid therapy (p=0.0183). The effect of cholestyramine, a
bile acid sequestrant, was evaluated for the ability to alter the fecal
bile acid pool in healthy dogs. The concentration of secondary
bile acids significantly increased in feces of healthy dogs administered cholestyramine (p=0.0183). These results demonstrate that a subset of dogs with chronic enteropathy show fecal
bile acid dysmetabolism, and further studies are warranted to evaluate the use of
bile acid sequestrants in clinical cases.
Advisors/Committee Members: Suchodolski, Jan S (advisor), Steiner, Jörg M (advisor), Jergens, Albert E (committee member), Webb, Craib B (committee member), Jayaraman, Arul (committee member).
Subjects/Keywords: bile acids; chronic enteropathy; inflammatory bowel disease; microbiome; primary bile acids; secondary bile acids; cholestyramine; bile acid sequestrants; GC/MS; untargeted metabolomics
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APA ·
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MLA ·
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CSE |
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APA (6th Edition):
Guard, B. C. (2017). Microbial Characterization, Metabolomic Profiling, and Bile Acid Metabolism in Healthy Dogs and Dogs with Chronic Enteropathy. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/165797
Chicago Manual of Style (16th Edition):
Guard, Blake Crosby. “Microbial Characterization, Metabolomic Profiling, and Bile Acid Metabolism in Healthy Dogs and Dogs with Chronic Enteropathy.” 2017. Doctoral Dissertation, Texas A&M University. Accessed March 08, 2021.
http://hdl.handle.net/1969.1/165797.
MLA Handbook (7th Edition):
Guard, Blake Crosby. “Microbial Characterization, Metabolomic Profiling, and Bile Acid Metabolism in Healthy Dogs and Dogs with Chronic Enteropathy.” 2017. Web. 08 Mar 2021.
Vancouver:
Guard BC. Microbial Characterization, Metabolomic Profiling, and Bile Acid Metabolism in Healthy Dogs and Dogs with Chronic Enteropathy. [Internet] [Doctoral dissertation]. Texas A&M University; 2017. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/1969.1/165797.
Council of Science Editors:
Guard BC. Microbial Characterization, Metabolomic Profiling, and Bile Acid Metabolism in Healthy Dogs and Dogs with Chronic Enteropathy. [Doctoral Dissertation]. Texas A&M University; 2017. Available from: http://hdl.handle.net/1969.1/165797
7.
片山, 俊郎.
生体成分動態の数理モデルによる解析 : Mathematical modeling and analysis of the dynamics of an ingredient in organism; セイタイ セイブン ドウタイ ノ スウリ モデル ニヨル カイセキ.
Degree: Nara Institute of Science and Technology / 奈良先端科学技術大学院大学
URL: http://hdl.handle.net/10061/4436
Subjects/Keywords: a bile acid dynamics model
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APA ·
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APA (6th Edition):
片山, . (n.d.). 生体成分動態の数理モデルによる解析 : Mathematical modeling and analysis of the dynamics of an ingredient in organism; セイタイ セイブン ドウタイ ノ スウリ モデル ニヨル カイセキ. (Thesis). Nara Institute of Science and Technology / 奈良先端科学技術大学院大学. Retrieved from http://hdl.handle.net/10061/4436
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
片山, 俊郎. “生体成分動態の数理モデルによる解析 : Mathematical modeling and analysis of the dynamics of an ingredient in organism; セイタイ セイブン ドウタイ ノ スウリ モデル ニヨル カイセキ.” Thesis, Nara Institute of Science and Technology / 奈良先端科学技術大学院大学. Accessed March 08, 2021.
http://hdl.handle.net/10061/4436.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
片山, 俊郎. “生体成分動態の数理モデルによる解析 : Mathematical modeling and analysis of the dynamics of an ingredient in organism; セイタイ セイブン ドウタイ ノ スウリ モデル ニヨル カイセキ.” Web. 08 Mar 2021.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Vancouver:
片山 . 生体成分動態の数理モデルによる解析 : Mathematical modeling and analysis of the dynamics of an ingredient in organism; セイタイ セイブン ドウタイ ノ スウリ モデル ニヨル カイセキ. [Internet] [Thesis]. Nara Institute of Science and Technology / 奈良先端科学技術大学院大学; [cited 2021 Mar 08].
Available from: http://hdl.handle.net/10061/4436.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.
Council of Science Editors:
片山 . 生体成分動態の数理モデルによる解析 : Mathematical modeling and analysis of the dynamics of an ingredient in organism; セイタイ セイブン ドウタイ ノ スウリ モデル ニヨル カイセキ. [Thesis]. Nara Institute of Science and Technology / 奈良先端科学技術大学院大学; Available from: http://hdl.handle.net/10061/4436
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.
University of Rochester
8.
Christian, Whitney V.; Ballatori, Nazzareno.
Identification of Residues Required for Functional
Interaction of the Two Subunits of the Organic Solute Transporter,
OSTα-OSTβ.
Degree: PhD, 2013, University of Rochester
URL: http://hdl.handle.net/1802/26814
► The organic solute transporter (OST) alpha-beta (OSTα-OSTβ) is a bile acid and steroid conjugate transporter. The transporter is composed of two distinct subunits, OSTα and…
(more)
▼ The organic solute transporter (OST) alpha-beta
(OSTα-OSTβ) is a bile acid and
steroid conjugate transporter. The
transporter is composed of two distinct
subunits, OSTα and OSTβ,
which heterodimerize to form a functional transport
unit.
Characterization of OSTα-deficient mice revealed that OSTα-OSTβ
plays a
major role in bile acid homeostasis as a key basolateral
efflux transporter in the
enterohepatic and renal-hepatic
circulation of bile acids. The phenotype of
OSTα-/- mice also
indicates that inactivation of the transporter may be
therapeutically beneficial, as these animals excrete more lipids in
their feces and
are protected against the hepatotoxic effects of
cholestasis. These observations
primed the studies described
herein that attempt to understand how the two
subunits work
together to generate transport activity and elucidate strategies
of
inhibition. The specific purpose of this research was to
determine the contribution
of the OSTβ subunit to the
holotransporter and identify key regions and amino
acids within
the peptide responsible for the functional roles it performs.
Through
the use of site-directed mutagenesis and several
techniques to measure
heterodimerization, cell surface
localization, and transport activity, this was
accomplished. Of
significance, the transmembrane domain region of OSTβ was
found to
be a major OSTα binding site absolutely required for
heterodimerization.
Interaction with OSTα is also dependent on the
membrane orientation of OSTβ,
which is correctly established by
specific positively charged residues in the Cxi
terminus.
Furthermore, the C-terminal region of the peptide was found to be
an
additional, but not obligatory site of interaction with OSTα.
Mutation of the highly
conserved Trp-Asn sequence at the
N-terminus of the OSTβ transmembrane
helix resulted in a
heteromeric transporter complex that reached the cell surface
but
was functionally dead. This novel finding suggests that OSTβ
directly
participates in the transport mechanism and specific
residues within its
transmembrane domain are critical for the
production of transport activity. Thus, it
appears that OSTβ is
more than an OSTα trafficking chaperone and comprises
part of the
functional transport unit. Overall, this study describes the first
thorough dissection of OSTβ and identifies residues required for
the functional
interaction of the two subunits, which comprise the
OST heteromer.
Subjects/Keywords: Bile Acid; Transporter; Organic Solute Transporter; OSTβ; OSTα.
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APA (6th Edition):
Christian, Whitney V.; Ballatori, N. (2013). Identification of Residues Required for Functional
Interaction of the Two Subunits of the Organic Solute Transporter,
OSTα-OSTβ. (Doctoral Dissertation). University of Rochester. Retrieved from http://hdl.handle.net/1802/26814
Chicago Manual of Style (16th Edition):
Christian, Whitney V.; Ballatori, Nazzareno. “Identification of Residues Required for Functional
Interaction of the Two Subunits of the Organic Solute Transporter,
OSTα-OSTβ.” 2013. Doctoral Dissertation, University of Rochester. Accessed March 08, 2021.
http://hdl.handle.net/1802/26814.
MLA Handbook (7th Edition):
Christian, Whitney V.; Ballatori, Nazzareno. “Identification of Residues Required for Functional
Interaction of the Two Subunits of the Organic Solute Transporter,
OSTα-OSTβ.” 2013. Web. 08 Mar 2021.
Vancouver:
Christian, Whitney V.; Ballatori N. Identification of Residues Required for Functional
Interaction of the Two Subunits of the Organic Solute Transporter,
OSTα-OSTβ. [Internet] [Doctoral dissertation]. University of Rochester; 2013. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/1802/26814.
Council of Science Editors:
Christian, Whitney V.; Ballatori N. Identification of Residues Required for Functional
Interaction of the Two Subunits of the Organic Solute Transporter,
OSTα-OSTβ. [Doctoral Dissertation]. University of Rochester; 2013. Available from: http://hdl.handle.net/1802/26814
University of Rochester
9.
Gorman-Wheeler, Sadie Grayce.
Mice Lacking the Organic Solute Transporter Ostα-Ostβ
have Altered Lipid and Glucose Homeostasis.
Degree: PhD, 2014, University of Rochester
URL: http://hdl.handle.net/1802/28973
► The organic solute transporter alpha-beta (Ostα-Ostβ) is an important transporter in the enterohepatic circulation of bile acids. Located on the basolateral side of ileal enterocytes,…
(more)
▼ The organic solute transporter alpha-beta
(Ostα-Ostβ) is an important transporter in the
enterohepatic
circulation of bile acids. Located on the basolateral side of
ileal
enterocytes, it mediates the efflux of bile acids from
enterocytes into portal circulation for
return to the liver.
Ostα-Ostβ mice have reduced bile acid synthesis, as evidenced by
lower
expression of the rate-limiting enzyme in the classical
pathway of bile acid synthesis,
Cyp7a1, and their bile acid pool
size is greatly diminished. Consistent with reduced bile
acid
levels, these mice have an impairment in dietary lipid absorption.
These studies
aimed to characterize Ostα-Ostβ mice in terms of
their lipid accumulation and glucose
homeostasis. Because wild
type C57Bl6 mice exhibit weight gain and insulin
desensitization
with age and initial studies in neonatal mice indicated a growth
retardation in Ostα-Ostβ mice, we hypothesized that Ostα-Ostβ mice
are resistant to the agerelated
weight gain and insulin
desensitization that wild type mice exhibit. Interestingly,
particularly in males, Ostα-Ostβ mice gained less weight with age
than wild type mice, had
smaller fat pads at 12 months, did not
accumulate increased liver lipids or exhibit insulin
desensitization with age, and lived slightly longer than wild type
mice. At both 5 and 12
months, male Ostα-Ostβ mice had increased
fecal lipid levels and had lower muscle
triglyceride content, and
at 5 months, had slightly improved glucose tolerance over wild
type. Insulin-stimulated AKT phosphorylation was measured in livers
and muscle tissue
from 12 month mice, and both male and female
Ostα-Ostβ mice showed greater insulin
responsiveness. Food
consumption, energy expenditure, and activity levels appeared to
be normal, suggesting that impaired dietary lipid absorption is an
important factor in
decreased lipid accumulation. Gene expression
analysis showed changes consistent with
decreased dietary lipid
absorption, with decreased expression of the cholesterol uptake
transporter Npc1L1 in the distal portion of the small intestine,
and decreased expression
of the cholesterol efflux tranporter
Abca1 in each section of the small intestine, each
observed at
both 5 and 12 months of age. In addition, hepatic expression of
the
cholesterol synthesis enzymes Hmgcr, Mvd, and Fdft was
increased, suggesting
cholesterol synthesis may be increased to
compensate for decreased absorption from the
diet. Together, these
data indicate that Ost!-/- mice are resistant to many of the
agerelated
impairments in lipid and glucose homeostasis that wild
type mice exhibit and
suggest that impaired dietary lipid
absorption is an important factor in these changes. To
test
whether Ostα-Ostβ mice are resistant to diet-induced weight gain,
mice were placed on a
high fat western diet or a low fat diet
control. Interestingly, while Ostα-Ostβ mice on the low
fat diet
control were significantly smaller than wild type mice, after 12
weeks on the
western diet, there was no significant difference in
body weight even though…
Subjects/Keywords: Bile Acid; Glucose Homeostasis; Lipid Homeostasis; Insulin Sensitivity; High Fat Diet
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Manager
APA (6th Edition):
Gorman-Wheeler, S. G. (2014). Mice Lacking the Organic Solute Transporter Ostα-Ostβ
have Altered Lipid and Glucose Homeostasis. (Doctoral Dissertation). University of Rochester. Retrieved from http://hdl.handle.net/1802/28973
Chicago Manual of Style (16th Edition):
Gorman-Wheeler, Sadie Grayce. “Mice Lacking the Organic Solute Transporter Ostα-Ostβ
have Altered Lipid and Glucose Homeostasis.” 2014. Doctoral Dissertation, University of Rochester. Accessed March 08, 2021.
http://hdl.handle.net/1802/28973.
MLA Handbook (7th Edition):
Gorman-Wheeler, Sadie Grayce. “Mice Lacking the Organic Solute Transporter Ostα-Ostβ
have Altered Lipid and Glucose Homeostasis.” 2014. Web. 08 Mar 2021.
Vancouver:
Gorman-Wheeler SG. Mice Lacking the Organic Solute Transporter Ostα-Ostβ
have Altered Lipid and Glucose Homeostasis. [Internet] [Doctoral dissertation]. University of Rochester; 2014. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/1802/28973.
Council of Science Editors:
Gorman-Wheeler SG. Mice Lacking the Organic Solute Transporter Ostα-Ostβ
have Altered Lipid and Glucose Homeostasis. [Doctoral Dissertation]. University of Rochester; 2014. Available from: http://hdl.handle.net/1802/28973
University of Pretoria
10.
Viljoen, Adrienne.
Coagulation,
oncotic and haemodilutional effects of a third generation
hydroxyethyl starch (130/0.4) in ponies.
Degree: Companion Animal Clinical
Studies, 2014, University of Pretoria
URL: http://hdl.handle.net/2263/40705
► This dissertation describes the effects of tetrastarch (130/0.4) on serum colloid osmotic pressure and thromboelastography variables in healthy pony mares. Additional variables assessed during this…
(more)
▼ This dissertation describes the effects of tetrastarch
(130/0.4) on serum colloid
osmotic pressure and
thromboelastography variables in healthy pony mares.
Additional
variables assessed during this study included markers of
haemodilution
(PCV, TS) and serum creatinine and
bile acid
concentrations. Six clinically healthy Nooitgedacht pony mares were
utilized in a crossover study
design. Tetrastarch (130/0.4) was
administered at 10, 20 and 40 ml/kg bwt to each
mare in a random
sequence with a two week washout period between each of the
treatments. Packed cell volume (PCV), plasma total solids (TS),
serum colloid
osmotic pressure (COP), and platelet count were
measured and thromboelastography
(TEG) was performed before
treatment (baseline), immediately after infusion (time
0), and 1,
6, 12, 24, 48, and 96 h after tetrastarch infusion.
All TEG
variables remained within reference range in all treatment groups.
Administration of tetrastarch at 40 ml/kg bwt resulted in a
prolonged K-time at 6 h
post-infusion, and decreased maximum
amplitude at 0, 1, 6, 24 and 48 h post-infusion
compared to
baseline. Administration of tetrastarch increased mean COP values
above baseline in all three treatment groups, persisting to 24, 6
and 48 h after
treatment with 10, 20 and 40 ml/kg of tetrastarch
respectively.
This study concluded that, although values remained
within established reference
ranges, the administration of
tetrastarch (130/0.4) at 40 ml/kg bwt is more likely to
induce
changes in TEG variables than doses of 20 ml/kg or less.
Tetrastarch increased
COP in healthy horses at all evaluated dose
rates.
Advisors/Committee Members: Saulez, M.N. (advisor), Page, Patrick Collin (coadvisor).
Subjects/Keywords: Horses; Pony
mares;
Thromboelastography variables; Bile acid
concentrations;
UCTD
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APA ·
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MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Viljoen, A. (2014). Coagulation,
oncotic and haemodilutional effects of a third generation
hydroxyethyl starch (130/0.4) in ponies. (Masters Thesis). University of Pretoria. Retrieved from http://hdl.handle.net/2263/40705
Chicago Manual of Style (16th Edition):
Viljoen, Adrienne. “Coagulation,
oncotic and haemodilutional effects of a third generation
hydroxyethyl starch (130/0.4) in ponies.” 2014. Masters Thesis, University of Pretoria. Accessed March 08, 2021.
http://hdl.handle.net/2263/40705.
MLA Handbook (7th Edition):
Viljoen, Adrienne. “Coagulation,
oncotic and haemodilutional effects of a third generation
hydroxyethyl starch (130/0.4) in ponies.” 2014. Web. 08 Mar 2021.
Vancouver:
Viljoen A. Coagulation,
oncotic and haemodilutional effects of a third generation
hydroxyethyl starch (130/0.4) in ponies. [Internet] [Masters thesis]. University of Pretoria; 2014. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/2263/40705.
Council of Science Editors:
Viljoen A. Coagulation,
oncotic and haemodilutional effects of a third generation
hydroxyethyl starch (130/0.4) in ponies. [Masters Thesis]. University of Pretoria; 2014. Available from: http://hdl.handle.net/2263/40705
Boston University
11.
Hafften, Nicholas.
Dysbiosis in inflammatory bowel disease promotes clostridium difficile colonization.
Degree: MS, Medical Sciences, 2015, Boston University
URL: http://hdl.handle.net/2144/16134
► Research into the gut microbiome has revealed the widespread influence that microbial species have on their host. Host genetics and environmental factors influence the abundance…
(more)
▼ Research into the gut microbiome has revealed the widespread influence that microbial species have on their host. Host genetics and environmental factors influence the abundance and diversity of the bacterial species living within the gastrointestinal tract. When the normal composition of the gut microbiota is altered, a dysbiotic state incurs. Inflammatory bowel disease (IBD) is a chronic/relapsing inflammatory disorder of the intestinal mucosa, which is characterized by a state of dysbiosis. Despite the large amount of information studying the role dysbiosis has in the pathogenesis of IBD, it is not clear how the altered microbial composition of the gut in IBD patients leads to susceptibility to enteric pathogens such as Clostridium difficile. This study aims to highlight the features of the gastrointestinal tract that are modified as a result of dysbiosis in the IBD population, and how these features facilitate colonization by C. difficile and symptom development. Review of the available literature demonstrated that the depletion of Clostridial cluster XIVa in IBD-associated dysbiosis alters bile acid metabolism and butyrate fermentation in the colon, ultimately promoting germination of C. difficile spores and weakening the gut's immune response against toxin-mediated inflammation. From continued research into the gut microbiota, more will be understood of how these microbial organisms influence human health and disease.
Subjects/Keywords: Medicine; Clostridium difficile; Gut microbiota; Inflammatory bowel disease; Bile acid; Butyrate
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Chicago ·
MLA ·
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CSE |
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to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Hafften, N. (2015). Dysbiosis in inflammatory bowel disease promotes clostridium difficile colonization. (Masters Thesis). Boston University. Retrieved from http://hdl.handle.net/2144/16134
Chicago Manual of Style (16th Edition):
Hafften, Nicholas. “Dysbiosis in inflammatory bowel disease promotes clostridium difficile colonization.” 2015. Masters Thesis, Boston University. Accessed March 08, 2021.
http://hdl.handle.net/2144/16134.
MLA Handbook (7th Edition):
Hafften, Nicholas. “Dysbiosis in inflammatory bowel disease promotes clostridium difficile colonization.” 2015. Web. 08 Mar 2021.
Vancouver:
Hafften N. Dysbiosis in inflammatory bowel disease promotes clostridium difficile colonization. [Internet] [Masters thesis]. Boston University; 2015. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/2144/16134.
Council of Science Editors:
Hafften N. Dysbiosis in inflammatory bowel disease promotes clostridium difficile colonization. [Masters Thesis]. Boston University; 2015. Available from: http://hdl.handle.net/2144/16134
Bucknell University
12.
Nelson, Brandon Nathaniel.
Development of a Stereoselective Approach for the Synthesis of Allo Bile Acids.
Degree: 2016, Bucknell University
URL: https://digitalcommons.bucknell.edu/masters_theses/167
► Bile acids are natural products that are located within a variety of organisms through metabolism of cholesterol. The less common allo bile acids contain a…
(more)
▼ Bile acids are natural products that are located within a variety of organisms through metabolism of cholesterol. The less common allo bile acids contain a trans AB ring fusion and are designated as the C5α diastereomers. Although Iqbal, and Tochtrop have described synthetic approaches to the 5α stereocenter, there still remain issues of stereoselectivity and chemoselectivity when forging the AB trans ring. We have established a general approach to the synthesis of allo bile acids by applying a recently developed manganese catalyzed hydrogen atom transfer reduction. This key tactic enabled us to successfully synthesize allolithocholic acid over seven steps in 26% yield and 97% diastereomer purity, starting with the commercially available lithocholic acid. Notably, this is the first reported synthesis of allolithocholic acid. Derivatives of lithocholic acid were also synthesized to explore the hydrogen atom transfer reaction in order to develop an understanding of the reaction outcome in terms of stereoselectivity. Finally, this synthetic tactic was also applied to the synthesis of other allo bile acid derivatives such as deoxycholic, chenodeoxycholic, and hyodeoxycholic acids.
The later chapter in this thesis describes a synthetic endeavor to develop a general approach toward the eudesmane carbon skeleton through a double addition strategy. This strategy was effective in forming the C10 quaternary center and the C6–C7 bond in the eudesmane skeleton albeit with modest stereoselectivity as a 1:1 mixture of uncharacterized diastereomers.
Subjects/Keywords: organic synthesis; bile acid; eudesmane; hydrogen atom transfer; stereoselective
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Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Nelson, B. N. (2016). Development of a Stereoselective Approach for the Synthesis of Allo Bile Acids. (Thesis). Bucknell University. Retrieved from https://digitalcommons.bucknell.edu/masters_theses/167
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Nelson, Brandon Nathaniel. “Development of a Stereoselective Approach for the Synthesis of Allo Bile Acids.” 2016. Thesis, Bucknell University. Accessed March 08, 2021.
https://digitalcommons.bucknell.edu/masters_theses/167.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Nelson, Brandon Nathaniel. “Development of a Stereoselective Approach for the Synthesis of Allo Bile Acids.” 2016. Web. 08 Mar 2021.
Vancouver:
Nelson BN. Development of a Stereoselective Approach for the Synthesis of Allo Bile Acids. [Internet] [Thesis]. Bucknell University; 2016. [cited 2021 Mar 08].
Available from: https://digitalcommons.bucknell.edu/masters_theses/167.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Nelson BN. Development of a Stereoselective Approach for the Synthesis of Allo Bile Acids. [Thesis]. Bucknell University; 2016. Available from: https://digitalcommons.bucknell.edu/masters_theses/167
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Toronto
13.
Stankovic, Bogdan.
Regulatory Role of Bile Acids in Dietary Fat Absorption and Intestinal Lipoprotein Production.
Degree: 2019, University of Toronto
URL: http://hdl.handle.net/1807/98373
► Postprandial dyslipidemia is a key feature of insulin resistance that involves the overproduction of chylomicrons from the intestine after a meal, resulting in an excess…
(more)
▼ Postprandial dyslipidemia is a key feature of insulin resistance that involves the overproduction of chylomicrons from the intestine after a meal, resulting in an excess number of atherogenic lipoprotein remnants. Bile acids have been recognized as signaling molecules with the ability to regulate peripheral lipid metabolism and energy expenditure. Here I examined the utility of intraduodenal bile acid administration for lowering postprandial lipemia and chylomicron production in the Syrian golden hamster model. Bile acid administration was shown to decrease postprandial lipemia by slowing down gastric emptying and decreasing mRNA expression of genes involved in fatty acid absorption and chylomicron assembly. Furthermore, bile acid administration was able to regulate VLDL secretion in the fasting state and decreased postprandial lipemia in insulin resistant hamsters. Altogether, the findings arising from these studies support the potential utility of targeting bile acid receptors in the treatment of postprandial dyslipidemia often seen in insulin resistant states.
M.Sc.
Advisors/Committee Members: Adeli, Khosrow, Laboratory Medicine and Pathobiology.
Subjects/Keywords: bile acid; chylomicron; dyslipidemia; intestinal; lipoprotein; postprandial; 0719
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MLA ·
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CSE |
Export
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Manager
APA (6th Edition):
Stankovic, B. (2019). Regulatory Role of Bile Acids in Dietary Fat Absorption and Intestinal Lipoprotein Production. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/98373
Chicago Manual of Style (16th Edition):
Stankovic, Bogdan. “Regulatory Role of Bile Acids in Dietary Fat Absorption and Intestinal Lipoprotein Production.” 2019. Masters Thesis, University of Toronto. Accessed March 08, 2021.
http://hdl.handle.net/1807/98373.
MLA Handbook (7th Edition):
Stankovic, Bogdan. “Regulatory Role of Bile Acids in Dietary Fat Absorption and Intestinal Lipoprotein Production.” 2019. Web. 08 Mar 2021.
Vancouver:
Stankovic B. Regulatory Role of Bile Acids in Dietary Fat Absorption and Intestinal Lipoprotein Production. [Internet] [Masters thesis]. University of Toronto; 2019. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/1807/98373.
Council of Science Editors:
Stankovic B. Regulatory Role of Bile Acids in Dietary Fat Absorption and Intestinal Lipoprotein Production. [Masters Thesis]. University of Toronto; 2019. Available from: http://hdl.handle.net/1807/98373
University of Bridgeport
14.
Molien, Gabrielle M.
The Role of Bile Acids in Cholecystectomy and Colorectal Cancer
.
Degree: 2015, University of Bridgeport
URL: https://scholarworks.bridgeport.edu/xmlui/handle/123456789/1430
► Digestive diseases have been reported by the CDC as the third largest illness of great economic burden in the United States. The cost of gallbladder…
(more)
▼ Digestive diseases have been reported by the CDC as the third largest illness of great economic burden in the United States. The cost of gallbladder disease causing digestive illness has been estimated to be more than a billion of dollars in direct medical care. The disease occurs in 10% of the population and is higher among women and elderly. About 776,000 patients was diagnosed with gallstones in 1984 and 485,000 of these patients received a cholecystectomy. New prevention model consisting of risks management, screening exam, and ultrasound standard procedure were put in place in the hope of better management and prevention of gallstones disease. NHANES III have included ultrasound of the gallbladder has one of the digestive diseases diagnostic component. The procedure is performed to assess abnormalities within the gallbladder more specifically the presence of stones in 20 to 74 year old patients. This research thesis will try to decipher the importance of bile acids in digestion, gallstones formation, its many purposes in human metabolism, its detrimental effect on digestion after cholecystectomy, and its role if any in the development of colorectal cancer.
Subjects/Keywords: Naturopathy;
Bile acid;
Cholecystectomy;
Digestive diseases;
Colorectal cancer
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to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Molien, G. M. (2015). The Role of Bile Acids in Cholecystectomy and Colorectal Cancer
. (Thesis). University of Bridgeport. Retrieved from https://scholarworks.bridgeport.edu/xmlui/handle/123456789/1430
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Molien, Gabrielle M. “The Role of Bile Acids in Cholecystectomy and Colorectal Cancer
.” 2015. Thesis, University of Bridgeport. Accessed March 08, 2021.
https://scholarworks.bridgeport.edu/xmlui/handle/123456789/1430.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Molien, Gabrielle M. “The Role of Bile Acids in Cholecystectomy and Colorectal Cancer
.” 2015. Web. 08 Mar 2021.
Vancouver:
Molien GM. The Role of Bile Acids in Cholecystectomy and Colorectal Cancer
. [Internet] [Thesis]. University of Bridgeport; 2015. [cited 2021 Mar 08].
Available from: https://scholarworks.bridgeport.edu/xmlui/handle/123456789/1430.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Molien GM. The Role of Bile Acids in Cholecystectomy and Colorectal Cancer
. [Thesis]. University of Bridgeport; 2015. Available from: https://scholarworks.bridgeport.edu/xmlui/handle/123456789/1430
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Louisiana State University
15.
Moncada Reyes, Marvin L.
Influence of low sonication intensities at different temperatures on acid tolerance, bile tolerance, protease activity and growth of yogurt culture bacteria Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus salivarius ssp. thermophilus.
Degree: MS, Animal Sciences, 2011, Louisiana State University
URL: etd-03292011-141434
;
https://digitalcommons.lsu.edu/gradschool_theses/939
► Streptococcus salivarius ssp. thermophilus and Lactobacillus delbrueckii ssp. bulgaricus are dairy cultures widely used in the dairy industry. Low sonication intensity condition is a non-destructive…
(more)
▼ Streptococcus salivarius ssp. thermophilus and Lactobacillus delbrueckii ssp. bulgaricus are dairy cultures widely used in the dairy industry. Low sonication intensity condition is a non-destructive technique that uses sound waves to cause cavitation in aqueous solutions and may improve the permeability of membranes, speed up the transfer of substrates and promote cellular growth and propagation. The objective of this study was to determine the effect of low sonication intensities at different temperatures on acid tolerance, bile tolerance, protease activity and growth of the two dairy cultures. The cultures were freshly thawed and suspended in 0.1% peptone water and 18 ml of sample was sonicated using horn (diameter 13 mm) set at a maximum acoustic power output of 750 W, frequency 24 kHz. The treatments were four sonication intensities of 8.07, 14.68, 19.83 and 23.55 Watts/cm2 randomized at three different temperatures (4, 22 and 40°C) of inoculated peptone water before sonication. The energy input (1500 Joules) was kept constant in all treatments. Control samples did not receive any sonication treatment. Growth and bile tolerance of samples were determined hourly for 12 hours of incubation. Acid tolerance was determined for Streptococcus thermophilus every 5 minutes for 20 minutes of incubation and for Lactobacillus bulgaricus every minute for 5 minutes of incubation. Protease activity was determined at 0, 12 and 24 hours of incubation. The experimental design was a completely randomized design (CRD). Three replications were conducted for each experimental condition. Data were analyzed using Proc Mixed Model of Statistical Analysis System (SAS®). Differences of least square means were used to determine significant differences at P<0.05 for main effects (low sonication intensity, time and temperature) two way interaction effect (low sonication intensity * temperature and low sonication intensity * time) and three way interaction effects (low sonication intensity * time * temperature). Low sonication conditions include a) low sonication intensities, b) temperatures and c) times, all three of which played a role in influencing the desirable attributes of both microorganisms. Of all the low sonication intensities studied, 14.68 watts /cm2 had the best overall influence at certain time points for Streptococcus thermophilus improving its acid tolerance, bile tolerance and growth at 4°C, growth at 22°C, bile tolerance and growth at 40°C and improving the Lactobacillus bulgaricus bile tolerance and growth at 4°C, its acid tolerance and protease activity at 40°C. Low sonication intensity of 19.83 Watts/cm2 had the overall best influence at certain time points for acid tolerance of both microorganisms at 22°C. Low sonication intensity of 23.55 Watts/cm2 had the overall best influence at certain time points for protease activity of Streptococcus thermophilus at 40°C and Lactobacillus bulgaricus at 22°C. Some low sonication conditions improved certain characteristics of culture bacteria.
Subjects/Keywords: Protease Activity and Growth; Bile Tolerance; Acid Tolerance; Low Sonication Intensities
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Manager
APA (6th Edition):
Moncada Reyes, M. L. (2011). Influence of low sonication intensities at different temperatures on acid tolerance, bile tolerance, protease activity and growth of yogurt culture bacteria Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus salivarius ssp. thermophilus. (Masters Thesis). Louisiana State University. Retrieved from etd-03292011-141434 ; https://digitalcommons.lsu.edu/gradschool_theses/939
Chicago Manual of Style (16th Edition):
Moncada Reyes, Marvin L. “Influence of low sonication intensities at different temperatures on acid tolerance, bile tolerance, protease activity and growth of yogurt culture bacteria Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus salivarius ssp. thermophilus.” 2011. Masters Thesis, Louisiana State University. Accessed March 08, 2021.
etd-03292011-141434 ; https://digitalcommons.lsu.edu/gradschool_theses/939.
MLA Handbook (7th Edition):
Moncada Reyes, Marvin L. “Influence of low sonication intensities at different temperatures on acid tolerance, bile tolerance, protease activity and growth of yogurt culture bacteria Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus salivarius ssp. thermophilus.” 2011. Web. 08 Mar 2021.
Vancouver:
Moncada Reyes ML. Influence of low sonication intensities at different temperatures on acid tolerance, bile tolerance, protease activity and growth of yogurt culture bacteria Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus salivarius ssp. thermophilus. [Internet] [Masters thesis]. Louisiana State University; 2011. [cited 2021 Mar 08].
Available from: etd-03292011-141434 ; https://digitalcommons.lsu.edu/gradschool_theses/939.
Council of Science Editors:
Moncada Reyes ML. Influence of low sonication intensities at different temperatures on acid tolerance, bile tolerance, protease activity and growth of yogurt culture bacteria Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus salivarius ssp. thermophilus. [Masters Thesis]. Louisiana State University; 2011. Available from: etd-03292011-141434 ; https://digitalcommons.lsu.edu/gradschool_theses/939
University of Kansas
16.
Selwyn Samraj, Felcy Pavithra.
Alterations in bile acid homeostasis and drug metabolism in germ-free mice.
Degree: PhD, Pharmacology, Toxicology & Therapeutics, 2014, University of Kansas
URL: http://hdl.handle.net/1808/23957
► "We may be born 100% human but will die 90% bacterial – a truly complex organism!" (Goodacre, 2007). This statement reflects the fact that there are…
(more)
▼ "We may be born 100% human but will die 90% bacterial – a truly complex organism!" (Goodacre, 2007). This statement reflects the fact that there are 10 times more bacterial cells in the human body compared to the number of human cells, and there are 100 times more genes in the human microbiome compared to the number of genes in the human genome. Gut bacteria and host communicate with each other and collectively determine many aspects of host physiology such as
bile acid (BA) and drug metabolism. Gut bacteria varies significantly between individuals and therefore, may be responsible for the inter-individual differences in BA concentrations and drug responses. There are a number of diseases such as obesity, inflammatory bowel disorder, and autism, that have been associated with an abnormal bloom in certain gut bacteria or a decrease in the diversity of gut bacteria. Therefore, modulating gut bacteria by probiotics, prebiotics, and by fecal transplantation have become viable therapeutic strategies. Alterations of gut bacteria in diseases or the therapeutic modulation of gut bacteria has the potential to alter host BA signaling and drug responses. Germ-free (GF) mice provide an excellent model system for understanding the functions of gut bacteria. The overall goal of this dissertation is to expand the understanding of the role of gut bacteria in regulating host BA homeostasis and hepatic drug metabolism. In Specific Aim 1, I determined the changes in BA homeostasis and BA signaling in GF mice. BAs are amphipathic cholesterol metabolites that are synthesized in liver and secreted into
bile. Gut bacteria metabolize primary BAs to secondary BAs. The majority of BAs are reabsorbed from the intestine, effluxed into the portal vein and return to the liver. Therefore, the BA profile in the host is the result of the host hepatic enzyme activity and the gut bacterial enzyme activity. The BA profile is important because, BAs act like hormones and regulate host physiology by activating the BA receptors, namely the farnesoid X receptor (FXR) and transmembrane G-protein-coupled receptor (TGR5). The BA profiles of both male and female GF mice are markedly altered compared to conventional (CV) mice. GF mice have an increase in total BAs in all the tissue compartments analyzed and decreased total fecal excretion of BAs compared to CV mice. This could be due to slower intestinal propulsion rates and increased BA reabsorption from the intestines. The dominant BAs in GF mice are taurine conjugated α and β muricholic acids (Tα+β MCA). There is an increase in both ursodeoxycholic
acid (UDCA) and MCAs and in the proportion of taurine conjugated BAs and these BAs result in a more hydrophilic BA pool in GF mice. UDCA which was previously considered to be a secondary BA that is synthesized by gut bacteria increases in GF mice. Biotransformation experiments in vitro demonstrated that UDCA can be synthesized from CDCA by enzymes present in hepatic microsomes isolated from both GF and CV mice. This explains why UDCA is increased…
Advisors/Committee Members: Klaassen, Curtis D (advisor), Pazdernik, Thomas (advisor), Pazdernik, Thomas (cmtemember), Hagenbuch, Bruno (cmtemember), Blanco, Gustavo (cmtemember), Kasturi, Partha (cmtemember), Li, Tiangang (cmtemember).
Subjects/Keywords: Toxicology; Physiology; Bile acid; Drug metabolism; Germ-free mice
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APA (6th Edition):
Selwyn Samraj, F. P. (2014). Alterations in bile acid homeostasis and drug metabolism in germ-free mice. (Doctoral Dissertation). University of Kansas. Retrieved from http://hdl.handle.net/1808/23957
Chicago Manual of Style (16th Edition):
Selwyn Samraj, Felcy Pavithra. “Alterations in bile acid homeostasis and drug metabolism in germ-free mice.” 2014. Doctoral Dissertation, University of Kansas. Accessed March 08, 2021.
http://hdl.handle.net/1808/23957.
MLA Handbook (7th Edition):
Selwyn Samraj, Felcy Pavithra. “Alterations in bile acid homeostasis and drug metabolism in germ-free mice.” 2014. Web. 08 Mar 2021.
Vancouver:
Selwyn Samraj FP. Alterations in bile acid homeostasis and drug metabolism in germ-free mice. [Internet] [Doctoral dissertation]. University of Kansas; 2014. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/1808/23957.
Council of Science Editors:
Selwyn Samraj FP. Alterations in bile acid homeostasis and drug metabolism in germ-free mice. [Doctoral Dissertation]. University of Kansas; 2014. Available from: http://hdl.handle.net/1808/23957
University of Kansas
17.
Woolbright, Ben.
The role of bile acids during cholestasis in mice and man.
Degree: PhD, Pharmacology, Toxicology & Therapeutics, 2015, University of Kansas
URL: http://hdl.handle.net/1808/19455
► Cholestasis is a reduction in bile flow that occurs during numerous pathologies. Cholestasis leads to significant liver toxicity, biliary hyperplasia, and liver cirrhosis. The molecular…
(more)
▼ Cholestasis is a reduction in
bile flow that occurs during numerous pathologies. Cholestasis leads to significant liver toxicity, biliary hyperplasia, and liver cirrhosis. The molecular mechanisms behind the early liver injury associated with cholestasis are extensively studied, but the details, especially in man, are not well understood. The predominant hypothesis for the cause of cholestatic liver injury is that a buildup of toxic
bile acids in liver and serum leads to hepatocellular apoptosis. While the direct toxicity of
bile acids is supported by in vitro studies in primary rat hepatocytes and transfected human hepatoma lines, recent studies measuring the concentrations of individual
bile acids after cholestasis in vivo has led us to reevaluate mechanisms of cytotoxicity during cholestasis, as
bile acid levels in man may not reach the necessary concentration for onset of toxicity. Thus, the overarching goal of this dissertation project was to determine the effect of pathophysiologically relevant concentrations of
bile acids in man, with an emphasis on the human condition. This project is focused upon understanding the mechanisms and cellular events that determine how cholestasis results in liver injury with the hope of furthering understanding the progression of the injury in vivo both in human patients, human hepatocyte lines and murine models. This study resulted in a number of findings that are potentially significant to the field of cholestatic liver injury. Primarily, human hepatocytes are resistant to
bile acid induced apoptosis, and moreover human patients undergo relatively little apoptosis during cholestatic liver injury. This is likely due to a combination of dramatic differences between human and rodent
bile acid compositions and the pathophysiology of in vivo models versus in vitro modeling in rodents. Further work is necessary to fully ascertain how, and why, human hepatocytes and human patients undergo liver injury during cholestasis.
Advisors/Committee Members: Jaeschke, Hartmut (advisor), Apte, Udayan (cmtemember), Ding, Wen-Xing (cmtemember), Pazdernik, Thomas (cmtemember), Wood, John (cmtemember).
Subjects/Keywords: Toxicology; Pathology; Pharmacology; apoptosis; bile acid; cholestasis; hepatocytes; inflammation; neutrophil
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APA (6th Edition):
Woolbright, B. (2015). The role of bile acids during cholestasis in mice and man. (Doctoral Dissertation). University of Kansas. Retrieved from http://hdl.handle.net/1808/19455
Chicago Manual of Style (16th Edition):
Woolbright, Ben. “The role of bile acids during cholestasis in mice and man.” 2015. Doctoral Dissertation, University of Kansas. Accessed March 08, 2021.
http://hdl.handle.net/1808/19455.
MLA Handbook (7th Edition):
Woolbright, Ben. “The role of bile acids during cholestasis in mice and man.” 2015. Web. 08 Mar 2021.
Vancouver:
Woolbright B. The role of bile acids during cholestasis in mice and man. [Internet] [Doctoral dissertation]. University of Kansas; 2015. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/1808/19455.
Council of Science Editors:
Woolbright B. The role of bile acids during cholestasis in mice and man. [Doctoral Dissertation]. University of Kansas; 2015. Available from: http://hdl.handle.net/1808/19455
Indian Institute of Science
18.
Maity, Mitasree.
Bile Acid based Supramolecular Gels, Soft Hybrid Materials and their Applications.
Degree: PhD, Faculty of Science, 2017, Indian Institute of Science
URL: http://etd.iisc.ac.in/handle/2005/2928
► Chapter 1. Supramolecular Gels and their Applications Supramolecular gels are viscoelastic materials composed of a solid like three dimensional fibrillary network that is embedded in…
(more)
▼ Chapter 1. Supramolecular Gels and their Applications
Supramolecular gels are viscoelastic materials composed of a solid like three dimensional fibrillary network that is embedded in a liquid. Supramolecular gels are derived from low molecular weight compounds (typically MW < 3000). In the 1990s, the investigations on gels were mainly focused on designing new gelator molecules. However, during the last decade, research focus shifted towards designing functional gels and their applications. As a result of extensive work in this area, gels have been found to have varied applications in the templated synthesis of inorganic nanomaterials, hybrid materials, light harvesting systems, as responsive system and sensors, and also in drug delivery, tissue engineering etc. This chapter gives an introduction to supramolecular hydrogels/organogels and relevant
bile acid chemistry touching upon the gelation properties of the
bile acid derivatives. Diverse applications of the supramolecular gels are also illustrated with several examples.
Scheme 1. Various applications of functional supramolecular gels
Chapter 2.
Bile Acid derived novel Hydrogelators
Part 1. Hydrogelation of
Bile acid protected Amino acids and Hybrid Materials
Hydrogels from low molecular weight molecules have significant importance in biomedical applications. In this chapter, we report injectable hydrogel formation from
bile acid conjugates of various amino acids. Hydrogel formation was found to be dependent on multiple factors such as
bile acid backbone structure, linkage between the
bile acid and the amino
acid, pH etc. Single crystal structures of lithocholyl phenylalanine, lithocholyl-glycine, lithocholyl-L valine and lithocholyl-L alanine were also determined. Finally, the hydrogel frameworks were utilized to produce hybrid materials with Gold and ZnO nanoparticles.
Scheme 2. (a) Crystal structure of LC-LF-OH gelator molecule, (b) photograph of gel, (c) SEM and (d) AFM image of LC-LF-OH xerogel
Part 2. Hydrogelation of
bile acid-dipeptide conjugates and in situ synthesis
of silver and gold nanoparticles in the hydrogel matrix
Fabricating supramolecular hydrogels with embedded metal nanostructures are important for the design of novel hybrid nanocomposite materials for diverse applications such as bio sensing and chemo sensing platforms, catalytic and antibacterial functional materials etc. Supramolecular self-assembly of
bile acid-dipeptide conjugates have led to the formation of new supramolecular hydrogels. Gelation of these molecules depends strongly on the hydrophobic character of the
bile acids. Ag+ and Au3+ salts were incorporated in the hydrogels, and photo reduction and chemical reduction led to the in situ generation of Ag and Au NPs in these supramolecular hydrogels without the addition of any external stabilizing agent. The color, size and shape of silver nanoparticles formed by photo reduction depended on the amino
acid residue on the side chain. Furthermore, the hydrogel-Ag nanocomposite was tested for its antimicrobial…
Advisors/Committee Members: Maitra, Uday (advisor).
Subjects/Keywords: Supramolecular Gels; Soft Hybrid Materials; Bile Acids; Gelators; Bile Acid Hydrogelators; Sonogels; Supramolelcular Hydrogelation; Bile Acid Supramolecular Gels; Inorganic Nanomaterials; Supramolecular Hydrogels; Supramolecular Organogels; Bile Acid-Amino Acid Conjugates; Indium Sulfide Nanostructures; Palladium-Hydrogel Nanocomposite; Silver and Gold Nanoparticles; Organic Chemistry
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APA (6th Edition):
Maity, M. (2017). Bile Acid based Supramolecular Gels, Soft Hybrid Materials and their Applications. (Doctoral Dissertation). Indian Institute of Science. Retrieved from http://etd.iisc.ac.in/handle/2005/2928
Chicago Manual of Style (16th Edition):
Maity, Mitasree. “Bile Acid based Supramolecular Gels, Soft Hybrid Materials and their Applications.” 2017. Doctoral Dissertation, Indian Institute of Science. Accessed March 08, 2021.
http://etd.iisc.ac.in/handle/2005/2928.
MLA Handbook (7th Edition):
Maity, Mitasree. “Bile Acid based Supramolecular Gels, Soft Hybrid Materials and their Applications.” 2017. Web. 08 Mar 2021.
Vancouver:
Maity M. Bile Acid based Supramolecular Gels, Soft Hybrid Materials and their Applications. [Internet] [Doctoral dissertation]. Indian Institute of Science; 2017. [cited 2021 Mar 08].
Available from: http://etd.iisc.ac.in/handle/2005/2928.
Council of Science Editors:
Maity M. Bile Acid based Supramolecular Gels, Soft Hybrid Materials and their Applications. [Doctoral Dissertation]. Indian Institute of Science; 2017. Available from: http://etd.iisc.ac.in/handle/2005/2928
University of Western Ontario
19.
Russell, Laura E.
Human Genetic Variation in Na+-Taurocholate Co-transporting Polypeptide (NTCP; SLC10A1) and Targeted Slc10a1 Disruption in Mice: Effects on Bile Acid and Rosuvastatin Transport.
Degree: 2020, University of Western Ontario
URL: https://ir.lib.uwo.ca/etd/7311
► Sodium-taurocholate co-transporting polypeptide (NTCP, SLC10A1) is the central bile acid uptake transporter on the basolateral membrane of hepatocytes. Pharmacological inhibition of NTCP is also being…
(more)
▼ Sodium-taurocholate co-transporting polypeptide (NTCP, SLC10A1) is the central bile acid uptake transporter on the basolateral membrane of hepatocytes. Pharmacological inhibition of NTCP is also being used to treat Hepatitis B and D, and NTCP transports a variety of drugs including cholesterol-lowering statins. Despite these crucial roles, limited knowledge exists regarding the effects of genetic variation in SLC10A1 on bile acid and rosuvastatin transport.
To address this, we characterized activity and protein expression of genetically variant SLC10A1 in vitro. Seven SLC10A1 genetic variants displayed robust reductions in NTCP-mediated transport of taurocholic acid and rosuvastatin and virtually absent NTCP protein expression at the plasma membrane. In silico tools were employed to assess their performance to predict deleterious function, however these did not generate robust enough predictions to replace in vitro studies.
To elucidate the in vivo effects of targeted Slc10a1 disruption, serum bile acid composition and hepatic, renal, and ileal gene expression were assessed in male Slc10a1-/- mice. Conjugated serum hypercholanemia and absence of Oatp1a1 (Slco1a1) mRNA were observed in a subset of Slc10a1-/- mice. Additional changes in gene mRNA expression and mouse necropsy studies suggest these mice were unable to thrive as a result of nutrient malabsorption and disrupted nuclear receptor signaling.
Sex-related differences were evaluated in serum bile acid composition and hepatic gene expression in Slc10a1-/-mice. No important sex-related differences were observed in serum bile acid composition. Oatp1a1 mRNA was nearly undetectable in both male and female hypercholanemic mice. Sex associated differences in hepatic gene expression in control and normocholanemic Slc10a1-/-mice were consistent with literature, however these sex-specific differences were reversed for certain bile acid genes in hypercholanemic mice.
These findings identify novel loss of function genetic variants in the SLC10A1 gene in vitro. Additionally, our studies in Slc10a1-/-mice provide evidence of altered nuclear receptor signaling that may have important implications on bile acid physiology and drug response.
Subjects/Keywords: NTCP; SLC10A1; bile acid transport; rosuvastatin; transporters; genetic variation; Medical Pharmacology
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APA (6th Edition):
Russell, L. E. (2020). Human Genetic Variation in Na+-Taurocholate Co-transporting Polypeptide (NTCP; SLC10A1) and Targeted Slc10a1 Disruption in Mice: Effects on Bile Acid and Rosuvastatin Transport. (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/7311
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Russell, Laura E. “Human Genetic Variation in Na+-Taurocholate Co-transporting Polypeptide (NTCP; SLC10A1) and Targeted Slc10a1 Disruption in Mice: Effects on Bile Acid and Rosuvastatin Transport.” 2020. Thesis, University of Western Ontario. Accessed March 08, 2021.
https://ir.lib.uwo.ca/etd/7311.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Russell, Laura E. “Human Genetic Variation in Na+-Taurocholate Co-transporting Polypeptide (NTCP; SLC10A1) and Targeted Slc10a1 Disruption in Mice: Effects on Bile Acid and Rosuvastatin Transport.” 2020. Web. 08 Mar 2021.
Vancouver:
Russell LE. Human Genetic Variation in Na+-Taurocholate Co-transporting Polypeptide (NTCP; SLC10A1) and Targeted Slc10a1 Disruption in Mice: Effects on Bile Acid and Rosuvastatin Transport. [Internet] [Thesis]. University of Western Ontario; 2020. [cited 2021 Mar 08].
Available from: https://ir.lib.uwo.ca/etd/7311.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Russell LE. Human Genetic Variation in Na+-Taurocholate Co-transporting Polypeptide (NTCP; SLC10A1) and Targeted Slc10a1 Disruption in Mice: Effects on Bile Acid and Rosuvastatin Transport. [Thesis]. University of Western Ontario; 2020. Available from: https://ir.lib.uwo.ca/etd/7311
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
West Virginia University
20.
Shumar, Stephanie Anne.
Determining the Role of Nudt7 in the Regulation of Cellular CoA Levels and Metabolism.
Degree: PhD, Biochemistry, 2019, West Virginia University
URL: https://doi.org/10.33915/etd.3820
;
https://researchrepository.wvu.edu/etd/3820
► Coenzyme A (CoA) is an essential cofactor required for hundreds of metabolic processes. Because it is such a critical cofactor, CoA levels are tightly…
(more)
▼ Coenzyme A (CoA) is an essential cofactor required for hundreds of metabolic processes. Because it is such a critical cofactor, CoA levels are tightly regulated. In the fasted state and in diabetic mice, the concentration of CoA increases dramatically in the liver. This phenotype is associated with constitutively low CoA degradation, a process that is emerging as a potentially important mechanism for CoA regulation. Nudt7 and Nudt19 are two mammalian peroxisomal enzymes with CoA-degrading activity, which are highly expressed in the liver and kidney, respectively. Limited information is available on the biochemistry of Nudt7 and Nudt19; the structural basis for their distinct features and the extent to which Nudt7 contributes to maintaining homeostatic CoA levels in vivo are currently unknown. We used a combination of techniques including mutagenesis, molecular modeling, and enzymatic assays on purified proteins, plus metabolomics and measurement of fatty
acid oxidation in whole tissue homogenates and intact hepatocytes to: 1) characterize the biochemical, structural, and regulatory properties of Nudt7 and Nudt19 and 2) determine the effects that manipulations of Nudt7 expression have on CoA levels and lipid metabolism in mouse liver. This research establishes the importance of Nudt7-dependent CoA degradation in the regulation of select acyl-CoA species and the output of peroxisomal metabolic pathways such as
bile acid synthesis and peroxisomal fatty
acid oxidation.
Advisors/Committee Members: Roberta Leonardi, Frank B. Hillgartner, Frank B. Hillgartner.
Subjects/Keywords: Coenzyme A; Nudix hydrolase; liver metabolism; fatty acid oxidation; bile acid synthesis; Biochemistry
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APA (6th Edition):
Shumar, S. A. (2019). Determining the Role of Nudt7 in the Regulation of Cellular CoA Levels and Metabolism. (Doctoral Dissertation). West Virginia University. Retrieved from https://doi.org/10.33915/etd.3820 ; https://researchrepository.wvu.edu/etd/3820
Chicago Manual of Style (16th Edition):
Shumar, Stephanie Anne. “Determining the Role of Nudt7 in the Regulation of Cellular CoA Levels and Metabolism.” 2019. Doctoral Dissertation, West Virginia University. Accessed March 08, 2021.
https://doi.org/10.33915/etd.3820 ; https://researchrepository.wvu.edu/etd/3820.
MLA Handbook (7th Edition):
Shumar, Stephanie Anne. “Determining the Role of Nudt7 in the Regulation of Cellular CoA Levels and Metabolism.” 2019. Web. 08 Mar 2021.
Vancouver:
Shumar SA. Determining the Role of Nudt7 in the Regulation of Cellular CoA Levels and Metabolism. [Internet] [Doctoral dissertation]. West Virginia University; 2019. [cited 2021 Mar 08].
Available from: https://doi.org/10.33915/etd.3820 ; https://researchrepository.wvu.edu/etd/3820.
Council of Science Editors:
Shumar SA. Determining the Role of Nudt7 in the Regulation of Cellular CoA Levels and Metabolism. [Doctoral Dissertation]. West Virginia University; 2019. Available from: https://doi.org/10.33915/etd.3820 ; https://researchrepository.wvu.edu/etd/3820
Louisiana State University
21.
Vargas Lopez, Luis Alfonso.
Influence of "added" whey protein isolate on probiotic properties of yogurt culture bacteria and yogurt characteristics.
Degree: MS, Dairy Science, 2013, Louisiana State University
URL: etd-11152013-152635
;
https://digitalcommons.lsu.edu/gradschool_theses/780
► Consumers are becoming conscious of their diet, increasing protein intake and avoiding carbohydrates and fats. Whey proteins have branch chain amino acids responsible for muscle…
(more)
▼ Consumers are becoming conscious of their diet, increasing protein intake and avoiding carbohydrates and fats. Whey proteins have branch chain amino acids responsible for muscle building. Whey protein isolate (WPI) contains more than 90% protein. The effect of incremental addition of WPI on probiotic characteristics of pure cultures and cultures in yogurt and yogurt characteristics are not known. The hypothesis was that “added” WPI will influence the characteristics of yogurt culture bacteria in pure form and in yogurt. The objectives were: to determine the influence of added WPI on (1) acid and bile tolerance, growth and protease activity of pure cultures Streptococcus thermophilus ST-M5 and Lactobacillus bulgaricus LB-12, (2) growth, acid and bile tolerance of starter culture from manufactured plain yogurt, (3) the physico-chemical characteristics of yogurt over its shelf life and (4) the sensory attributes of yogurt. WPI was used at 0, 1, 2 and 3% w/v. Acid tolerance was conducted on pure cultures and cultures from manufactured plain yogurt at 30 minutes intervals for 2 hours of incubation and bile tolerance at 1 hour intervals for 5 hours. Yogurt was manufactured using 0 (control), 1, 2 and 3% WPI. For sensory evaluation, blueberry yogurt was manufactured using the same WPI concentrations. Physico-chemical analyses of yogurts were conducted every 7 days during 35 days of storage. Enumeration of yogurt cultures during yogurt´s shelf life was evaluated at 7, 21 and 35 days of storage. Sensory evaluation was conducted on yogurt 7 days after its manufacture. Data were analyzed using Proc Mixed model of SAS® 9.3 program and by analysis of variance (ANOVA) using Proc GLM. Significant differences between means were analyzed at α = 0.05 using Tukey´s adjustment. Use of 2% WPI improved acid tolerance of Streptococcus thermophilus ST-M5 in yogurt. Use of 2 and 3% WPI improved bile tolerance of Lactobacillus bulgaricus LB-12 over the 5 hours of incubation. WPI decreased syneresis of yogurts and improved sensory attributes of flavored yogurt. Overall liking scores were higher for 1% WPI yogurts compared to control. Overall, 1 or 2% WPI can be recommended in manufacture of higher whey protein yogurts.
Subjects/Keywords: Acid Tolerance; Yogurt; Protease Activity; Growth; Lactic Acid Bacteria; Bile Tolerance; Whey protein isolate
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APA (6th Edition):
Vargas Lopez, L. A. (2013). Influence of "added" whey protein isolate on probiotic properties of yogurt culture bacteria and yogurt characteristics. (Masters Thesis). Louisiana State University. Retrieved from etd-11152013-152635 ; https://digitalcommons.lsu.edu/gradschool_theses/780
Chicago Manual of Style (16th Edition):
Vargas Lopez, Luis Alfonso. “Influence of "added" whey protein isolate on probiotic properties of yogurt culture bacteria and yogurt characteristics.” 2013. Masters Thesis, Louisiana State University. Accessed March 08, 2021.
etd-11152013-152635 ; https://digitalcommons.lsu.edu/gradschool_theses/780.
MLA Handbook (7th Edition):
Vargas Lopez, Luis Alfonso. “Influence of "added" whey protein isolate on probiotic properties of yogurt culture bacteria and yogurt characteristics.” 2013. Web. 08 Mar 2021.
Vancouver:
Vargas Lopez LA. Influence of "added" whey protein isolate on probiotic properties of yogurt culture bacteria and yogurt characteristics. [Internet] [Masters thesis]. Louisiana State University; 2013. [cited 2021 Mar 08].
Available from: etd-11152013-152635 ; https://digitalcommons.lsu.edu/gradschool_theses/780.
Council of Science Editors:
Vargas Lopez LA. Influence of "added" whey protein isolate on probiotic properties of yogurt culture bacteria and yogurt characteristics. [Masters Thesis]. Louisiana State University; 2013. Available from: etd-11152013-152635 ; https://digitalcommons.lsu.edu/gradschool_theses/780
Massey University
22.
Archer, Richard Hamilton.
Hydrolysis of bile acid conjugates and dehydroxylation of cholic acid by Clostridium bifermentans : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Biotechnology
.
Degree: 1980, Massey University
URL: http://hdl.handle.net/10179/4586
► The transformation of bile acids by Clostridium bifermentans was studied with a view to developing a process whereby the bile acid conjugates of New Zealand…
(more)
▼ The transformation of bile acids by Clostridium bifermentans was studied with a view to developing a process whereby the bile acid conjugates of New Zealand mutton and beef gall may be converted to deoxycholic acid. Statistically designed experiments were employed to maximise 7α-dehydroxylation of cholic acid to deoxycholic acid and to minimise the 7α-dehydrogenation of cholic acid to 7-ketodeoxycholic acid. Both transformations showed optima near pH 7. High deoxycholate yields were associated with conditions less favourable to strong growth and with relatively high electrode potentials. 7-ketodeoxycholic acid production was not as sensitive to environmental factors as was 7α-de-hydroxylation and could not be eliminated merely by manipulating fermentation variables. Studies on the 7α-dehydrogenation of cholic acid with washed resting-stage cells of Cl. bifermentans indicated several means of manipulating 7-ketodeoxycholate yields which were then tested using batch fermentation. In the presence of Zn++ions, 7-ketodeoxycholate yields were reduced but dehydroxylation was completely inhibited. In the presence of EDTA, 7α-dehydrogenation was almost quantitative but deoxycholate yields were again nil. Both transformations were enhanced during aerobic incubation. The highest deoxycholate yield observed during the work (50 molar %) was obtained by sweeping the fermenter headspace with air. Growing cells of Cl. bifermentans effected the near-quantitative hydrolysis of glycodeoxycholate, taurodeoxycholate and taurocholate within 48 h whilst glycocholate was 90% deconjugated. At substrate concentrations greater than 0.1% w/v however, taurine conjugates were less well hydrolysed.
Subjects/Keywords: Clostridium bifermentans;
Bile acid;
Cholic acid
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APA (6th Edition):
Archer, R. H. (1980). Hydrolysis of bile acid conjugates and dehydroxylation of cholic acid by Clostridium bifermentans : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Biotechnology
. (Thesis). Massey University. Retrieved from http://hdl.handle.net/10179/4586
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Archer, Richard Hamilton. “Hydrolysis of bile acid conjugates and dehydroxylation of cholic acid by Clostridium bifermentans : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Biotechnology
.” 1980. Thesis, Massey University. Accessed March 08, 2021.
http://hdl.handle.net/10179/4586.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Archer, Richard Hamilton. “Hydrolysis of bile acid conjugates and dehydroxylation of cholic acid by Clostridium bifermentans : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Biotechnology
.” 1980. Web. 08 Mar 2021.
Vancouver:
Archer RH. Hydrolysis of bile acid conjugates and dehydroxylation of cholic acid by Clostridium bifermentans : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Biotechnology
. [Internet] [Thesis]. Massey University; 1980. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/10179/4586.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Archer RH. Hydrolysis of bile acid conjugates and dehydroxylation of cholic acid by Clostridium bifermentans : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Biotechnology
. [Thesis]. Massey University; 1980. Available from: http://hdl.handle.net/10179/4586
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Indian Institute of Science
23.
Ramesh, K.
A Study of Supramolecular Gels and Self Assembly of Novel Bile Acid Conjugates.
Degree: PhD, Faculty of Science, 2018, Indian Institute of Science
URL: http://etd.iisc.ac.in/handle/2005/3307
► Chapter 1: Functional and Responsive Supramolecular Gels In this chapter ‘supramolecular gels’ derived from small organic molecules with molecular mass of typically less than 2000…
(more)
▼ Chapter 1: Functional and Responsive Supramolecular Gels
In this chapter ‘supramolecular gels’ derived from small organic molecules with molecular mass of typically less than 2000 daltons are discussed. Representative examples of various low molecular weight gelators based on their natural availability and also divergent functionalities are mentioned (Scheme 1).
Scheme 1
Advances in the recent years have been very rapid in the field of supramolecular chemistry of gels giving rise to ‘Tunable responsive gels’. Control of the gel property in a reversible fashion has been the highlight of responsive gels. A few of the gels which are responsive towards various stimuli such as pH, photoirradiation, cations, anions, neutral species have been discussed.
Advances and scope of supramolecular gels in various applications have also been mentioned in detail with respective examples. Utilities of supramolecular gels in synthesis of nanostructures, in biology and medicine, enzyme recognition, catalysis etc are discussed. (Scheme 2).
Chapter 2: Charge transfer triggered organogels of bis(
bile acid)anthracene conjugates and 2,4.7-trinitrofluorenone.
In this chapter the study involves the synthesis of a special class of anthracene based steroidal derivatives. The appending of two amphiphilic
bile acid units imparts a unique hydrophobic/ hydrophilic balance on the chromophore. The 2,3-didecyloxyanthracene (DDOA) was reported to be a gelator of various organic solvents but none of the three
bile acid derivatives of anthracene synthesized was a gelator on its own. It was also observed that dialkoxy (propyl, heptyl, decyl) derivatives of anthracene formed strong charge-transfer gels in the presence of 2,4,7-trinitrofluorenone (TNF). The addition of electron deficient TNF to the steroidal derivatives of anthracene resulted in the gelation of some specific organic solvents. The driving force behind the gel formation resulted from the charge-transfer (CT) interaction between the electron rich anthracene and electron deficient fluorenone.
Figure 1. Chemical structures of 2,3-bis(
bile acid)anthracenes and TNF (centre), a scanning electronic microscopy image of xerogels prepared from bis(deoxycholyl)anthracene and TNF (left) and a photograph of the gel of bis(deoxycholyl)anthracene and TNF in n-octanol.
Thermochromic property (during sol to gel phase transition), absorption and variable temperature fluorescence measurements supported CT interaction. Thermal stability studies and dynamic rheology experiments confirmed that CT gels were thermally most stable and mechanically stronger with equi-molar amounts of the two components. Stiffness values obtained from rheological experiments also suggested that the gels were viscoelastic solids.
Chapter 3(A): Tb(III) sensitization in an organogel matrix: Selective luminescence quenching by an aromatic nitro derivative
In this chapter the discovery of metallo organogel formation by mixing methanolic solutions of Tb(OAc)3 and sodium deoxycholate (NaDCh) has been explored. Sensitization of…
Advisors/Committee Members: Maitra, Uday (advisor).
Subjects/Keywords: Supramolecular Gels; Novel Bile Acid Conjugates; Supramolecular Chemistry; Bile Acid Conjugates - Self Assembly; Functional Supramolecular Gels; Responsive Supramolecular Gels; Organogels; Lanthanide Cholate Gels; Bile Acid Click Conjugates; Bile Acid Derivatives; Organogel Matrix; Bile Acid Anthracece Conjugates; Metallogels; Hybrid Gels; Deoxycholate Gel Matrix; Lanthanide(III)‐Cholate Gels; Organic Chemistry
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MLA ·
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APA (6th Edition):
Ramesh, K. (2018). A Study of Supramolecular Gels and Self Assembly of Novel Bile Acid Conjugates. (Doctoral Dissertation). Indian Institute of Science. Retrieved from http://etd.iisc.ac.in/handle/2005/3307
Chicago Manual of Style (16th Edition):
Ramesh, K. “A Study of Supramolecular Gels and Self Assembly of Novel Bile Acid Conjugates.” 2018. Doctoral Dissertation, Indian Institute of Science. Accessed March 08, 2021.
http://etd.iisc.ac.in/handle/2005/3307.
MLA Handbook (7th Edition):
Ramesh, K. “A Study of Supramolecular Gels and Self Assembly of Novel Bile Acid Conjugates.” 2018. Web. 08 Mar 2021.
Vancouver:
Ramesh K. A Study of Supramolecular Gels and Self Assembly of Novel Bile Acid Conjugates. [Internet] [Doctoral dissertation]. Indian Institute of Science; 2018. [cited 2021 Mar 08].
Available from: http://etd.iisc.ac.in/handle/2005/3307.
Council of Science Editors:
Ramesh K. A Study of Supramolecular Gels and Self Assembly of Novel Bile Acid Conjugates. [Doctoral Dissertation]. Indian Institute of Science; 2018. Available from: http://etd.iisc.ac.in/handle/2005/3307
Kent State University
24.
Li, Tiangang.
PREGNANE X RECEPTOR REGULATION OF BILE ACID METABOLISM AND
CHOLESTEROL HOMEOSTASIS.
Degree: PhD, School of Biomedical Sciences, 2006, Kent State University
URL: http://rave.ohiolink.edu/etdc/view?acc_num=kent1132160196
► The nuclear receptor pregnane X receptor (PXR) is activated by bile acids, steroids and drugs and regulates a network of genes in lipid and drug…
(more)
▼ The nuclear receptor pregnane X receptor (PXR) is
activated by
bile acids, steroids and drugs and regulates a network
of genes in lipid and drug mechanisms. The goal of this study is to
investigate the role of PXR in the coordinate regulation of
bile
acid synthetic and detoxification genes and its implications in
cholestatic liver diseases and treatments. Cholesterol 7alpha
hydroxylase (CYP7A1) catalyzes the rate-limiting step in the
classic
bile acids synthetic pathway and plays a key role in
controlling
bile acids homeostasis. Quantitative real-time PCR
(Q-PCR) showed human PXR agonist rifampicin inhibited CYP7A1 mRNA
expression in primary human hepatocytes. Mammalian two-hybrid
assays, co-immunoprecipitation (co-IP) assays and chromatin
immunoprecipitation (ChIP) assay revealed that ligand-activated PXR
strongly interacted with HNF4alpha, the key activator of human
CYP7A1, and blocked HNF4alpha interaction with co-activator
PGC-1alpha, thus resulted in inhibition of CYP7A1. CYP3A4 is the
most abundant cytochrome P450 monooxygenase expressed in human
liver and intestine.
Bile acids and drugs-activated PXR induces
CYP3A4, which converts toxic
bile acids to non-toxic metabolites
for excretion. Studies using Q-PCR, reporter assays, GST pull-down
assays and ChIP assays revealed that PXR strongly induced CYP3A4
gene transcription by interacting with HNF4alpha, SRC-1 and
PGC-1alpha. SHP, a negative nuclear receptor, reduced PXR
recruitment of HNF4alpha and SRC-1 to the CYP3A4 chromatin and
inhibited CYP3A4. Interestingly, PXR concomitantly inhibited SHP
gene transcription and maximized the PXR induction of CYP3A4. Taken
together, PXR inhibits CYP7A1 to reduce
bile acid synthesis and
induces CYP3A4 to detoxify
bile acid. Thus, PXR may play a
protective role against cholestasis. Drugs targeted to PXR may be
developed for treating cholestatic liver diseases induced by
bile
acids and drugs. Mitochondrial sterol 27-hydroxylase (CYP27A1)
catalyzes the side-chain cleavage reaction in
bile acid synthetic
pathways and 27-hydroxylation of cholesterol mainly in the
peripheral tissues. Q-PCR revealed that rifampicin induced CYP27A1
mRNA expression in the intestine-derived Caco2 cells, but not in
primary human hepatocytes and HepG2 cells. Rifampicin stimulated
CYP27A1 gene transcription in cholesterol laden Caco2 cells and
increased intracellular 27HOC, which stimulates cholesterol efflux
by induction of cholesterol efflux transporters ABCA1 and ABCG1.
Mutagenesis analysis, electrophoretic mobility shift assay and ChIP
assays identified a functional PXR binding site in the human
CYP27A1 gene. These data suggest that 27-hydroxycholesterol is an
endogenous LXRalpha ligand and the PXR/CYP27A1/LXRalpha signaling
pathway regulate cholesterol efflux in intestine cells. Results
revealed an intestine-specific regulation of human CYP27A1 gene by
PXR and suggested a novel role for PXR in cholesterol metabolism
and detoxification in the intestine.
Advisors/Committee Members: Chiang, John (Advisor).
Subjects/Keywords: PXR; BILE; BILE ACID; HNF4¿¿¿¿; CYP7A1; CHOLESTEROL; rifampicin
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APA ·
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CSE |
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APA (6th Edition):
Li, T. (2006). PREGNANE X RECEPTOR REGULATION OF BILE ACID METABOLISM AND
CHOLESTEROL HOMEOSTASIS. (Doctoral Dissertation). Kent State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=kent1132160196
Chicago Manual of Style (16th Edition):
Li, Tiangang. “PREGNANE X RECEPTOR REGULATION OF BILE ACID METABOLISM AND
CHOLESTEROL HOMEOSTASIS.” 2006. Doctoral Dissertation, Kent State University. Accessed March 08, 2021.
http://rave.ohiolink.edu/etdc/view?acc_num=kent1132160196.
MLA Handbook (7th Edition):
Li, Tiangang. “PREGNANE X RECEPTOR REGULATION OF BILE ACID METABOLISM AND
CHOLESTEROL HOMEOSTASIS.” 2006. Web. 08 Mar 2021.
Vancouver:
Li T. PREGNANE X RECEPTOR REGULATION OF BILE ACID METABOLISM AND
CHOLESTEROL HOMEOSTASIS. [Internet] [Doctoral dissertation]. Kent State University; 2006. [cited 2021 Mar 08].
Available from: http://rave.ohiolink.edu/etdc/view?acc_num=kent1132160196.
Council of Science Editors:
Li T. PREGNANE X RECEPTOR REGULATION OF BILE ACID METABOLISM AND
CHOLESTEROL HOMEOSTASIS. [Doctoral Dissertation]. Kent State University; 2006. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=kent1132160196
Indian Institute of Science
25.
Bhat, Shreedhar.
Soft Materials Derived From Bile Acid Analogues.
Degree: PhD, Faculty of Science, 2009, Indian Institute of Science
URL: http://etd.iisc.ac.in/handle/2005/483
► Chapter 1. Introduction This chapter is an overview on the literature of self-association of small organic molecules. The chapter is presented in four parts. First,…
(more)
▼ Chapter 1. Introduction
This chapter is an overview on the literature of self-association of small organic molecules. The chapter is presented in four parts. First, an introduction to aggregation of small molecules is given with the emphasis on micelles and gels(Parts 1 and 2) In part 3, a short overview is given on
bile acid based aggregates and their applications. Lastly, the content of the thesis is outlined.
Chapter 2. Solution properties of novel cationic bil salts: A structure-aggregation property study
Scheme 1: Structures of Cationic
bile salts(Refer PDF File)
Bile Salts are biosurfactants and they are known to form micelles in aqueous medium. We studied the micellar properties of cationic
bile salts(Scheme 1) and compared with their natural (anionic) counterparts. A serendipitous discovery of the gelation of a cationic
bile salt(4) led us to investigate the aggregation properties of this new class of cationic hydrogelators. This chapter highlights the recent efforts on the study of side chain structure-aggregation property relationship of cationic
bile salts.
Bile acid analogues with a quaternary ammonium group(Scheme 1, compounds 2, 3, 4, 6, 8 and 12) on the side chain were found to efficiently gel aqueous salt solutions. Some of the cationic
bile salts gelled water alone and many of them gelled aqueous salt solutions even in the presence of organic co-solvents(≤ 20%) such as ethanol, methanol, DMSO and DMF. A strong counter ion dependent gelation was observed. These gels showed interconnected fibrous networks. Unlike natural anionic
bile salt gels(reported for NaDC, NaLC), the cationicgels reported here are pH independent. Cationic gels derived from DCA showed more solid-like rheological response compared to natural NaDC gels studied earlier by Tato et al. A strong structure(side-chain) andcounter-ion dependent flow of the cationic
bile salt gels was seen.
Chapter 3. Applications of cationic
bile salts and their aggregates
Cationic
bile salts are useful in many ways. We have studied some of the applications of cationic
bile salts(discussed in chapter 2) and their aggregates in this chapter. The chapter is presented in three parts.
Part 1. Interaction of Cationic
bile salts and DNA
The
bile acid amphiphilicity is believed to help the DNA binding process of polyamines. This has prompted us to study the DNA-
bile salt binding interaction of
bile salts. The binding of cationic
bile salts has been expressed in terms of C50 values, which were determined from the plot of fluorescence of ethidium bromide bound DNA vs.
bile salt concentration(Fig 1) The C50 values for cationic
bile salts were estimated to be about 1.2 mM.
Fig1: A plot of fluorescene of ethidium bromide bound DNA against
bile salt concentration (Refer PDF File)
Part 2. Cholesterol solubilization and crystallization studies in aqueous
bile salt solutions.
Dihydroxy
bile salt micelles are well known for cholesterol dissolution(e.g. UDCA and CDCA). We studied the dissolution of cholesterol in the cationic
bile salt micelles(of 21-25) and the…
Advisors/Committee Members: Maitra, Uday (advisor).
Subjects/Keywords: Bile Acid; Soft Materials; Cationic Bile Salts; Cationic Bile Salts - Applications; Bile Acid Derived Sulfur Analogues; Cationic Bile Salts - Properties; Cationic Hydrogelators; Bile Salt Micelles; Bile Acid Analogues; Organic Chemistry
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APA ·
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MLA ·
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CSE |
Export
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APA (6th Edition):
Bhat, S. (2009). Soft Materials Derived From Bile Acid Analogues. (Doctoral Dissertation). Indian Institute of Science. Retrieved from http://etd.iisc.ac.in/handle/2005/483
Chicago Manual of Style (16th Edition):
Bhat, Shreedhar. “Soft Materials Derived From Bile Acid Analogues.” 2009. Doctoral Dissertation, Indian Institute of Science. Accessed March 08, 2021.
http://etd.iisc.ac.in/handle/2005/483.
MLA Handbook (7th Edition):
Bhat, Shreedhar. “Soft Materials Derived From Bile Acid Analogues.” 2009. Web. 08 Mar 2021.
Vancouver:
Bhat S. Soft Materials Derived From Bile Acid Analogues. [Internet] [Doctoral dissertation]. Indian Institute of Science; 2009. [cited 2021 Mar 08].
Available from: http://etd.iisc.ac.in/handle/2005/483.
Council of Science Editors:
Bhat S. Soft Materials Derived From Bile Acid Analogues. [Doctoral Dissertation]. Indian Institute of Science; 2009. Available from: http://etd.iisc.ac.in/handle/2005/483
Indian Institute of Science
26.
Babu, P.
Synthesis And Physico-Chemical Properties Of Phosphonobile Acids : Novel Bile Acid Analogs.
Degree: PhD, Faculty of Science, 2011, Indian Institute of Science
URL: http://etd.iisc.ac.in/handle/2005/1314
Subjects/Keywords: Bile Acids; Phosphonobile Acid; Phosphonobile Salts; Organogelator; Cholylphosphonamidate; Bile Acid Analogs; Biochemistry
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APA ·
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MLA ·
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CSE |
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APA (6th Edition):
Babu, P. (2011). Synthesis And Physico-Chemical Properties Of Phosphonobile Acids : Novel Bile Acid Analogs. (Doctoral Dissertation). Indian Institute of Science. Retrieved from http://etd.iisc.ac.in/handle/2005/1314
Chicago Manual of Style (16th Edition):
Babu, P. “Synthesis And Physico-Chemical Properties Of Phosphonobile Acids : Novel Bile Acid Analogs.” 2011. Doctoral Dissertation, Indian Institute of Science. Accessed March 08, 2021.
http://etd.iisc.ac.in/handle/2005/1314.
MLA Handbook (7th Edition):
Babu, P. “Synthesis And Physico-Chemical Properties Of Phosphonobile Acids : Novel Bile Acid Analogs.” 2011. Web. 08 Mar 2021.
Vancouver:
Babu P. Synthesis And Physico-Chemical Properties Of Phosphonobile Acids : Novel Bile Acid Analogs. [Internet] [Doctoral dissertation]. Indian Institute of Science; 2011. [cited 2021 Mar 08].
Available from: http://etd.iisc.ac.in/handle/2005/1314.
Council of Science Editors:
Babu P. Synthesis And Physico-Chemical Properties Of Phosphonobile Acids : Novel Bile Acid Analogs. [Doctoral Dissertation]. Indian Institute of Science; 2011. Available from: http://etd.iisc.ac.in/handle/2005/1314
University of Helsinki
27.
Apalkova, Irina.
Prevalence and Diagnosis of Liver Diseases in Small Animal University Hospital 2007-2010 - a Retrospective Study.
Degree: Department of Equine and Small Animal Medicine; Helsingin yliopisto, Eläinlääketieteellinen tiedekunta, Kliinisen hevos- ja pieneläinlääketieteen osasto; Helsingfors universitet, Veterinärmedicinska fakulteten, Avdelningen för klinisk häst- och smådjursmedicin, 2013, University of Helsinki
URL: http://hdl.handle.net/10138/39611
► This retrospective study was done to find out the prevalence of different liver diseases in dogs and cats in the Small Animal University hospital during…
(more)
▼ This retrospective study was done to find out the prevalence of different liver diseases in dogs and cats in the Small Animal University hospital during a four year period 2007-10. This information is needed to plan further research on liver diseases, and the main source of interest for this are canine familial liver diseases associated with certain breeds. Therefore, the study looked for breeds that might be overrepresented with liver diseases. Breeds often described with familial liver diseases are e.g. Doberman, Dalmatian, cocker spaniel, Bedlington terrier and West Highland white terrier. Finally, this study looks into the diagnostic procedures for liver diseases in the hospital to compare them with current recommendations.
The patients' information was searched by different criteria from the hospitals' patient database (Provet). The initially found patients were included if they had been diagnosed or suspected with a liver disease, which also includes diseases of biliary tract or hepatic vasculature. Of these patients, basic information, possible diagnosis, relevant laboratory findings, ultrasound findings and biopsy results were collected. The data was collected in a worksheet in MS Excel and further analysed there and in PASW Statistics 18.0.
337 dogs and 36 cats were eventually included in the study, resulting in 1.24 % and 0.41 % prevalence of all liver diseases in the hospital population in dogs and cats respectively. 55 patients (15 %) did not get a certain diagnosis, although they were definitely found to have had some kind of liver disease. Primary diagnosis of 28 patients was something other than liver diseases, though liver was also affected to some extent. The most common hepatobiliary diseases in dogs were vascular disorders (80 patients and in cats cholangitis and cholangiohepatitis (11 patients). As for breed distribution, in dogs with vascular disorders miniature breeds stood out with extrahepatic portosystemic shunts, especially miniature schnauzers (2.13 % prevalence within breed). Shetland sheepdogs stood out with liver diseases in general (3.65 % prevalence within breed) and especially with the diseases of the biliary tract (4 dogs). There were not as many dogs of known risk breed f chronic hepatitis as one would have expected, no breed was represented by over 5 dogs with chronic liver diseases. Copper accumulation was found in only 6 dogs, all of different breeds. Serum ALP, ALT, total protein, albumin, urea and bilirubin had been measured from nearly all of the patients in this study. Bile acids were measured from 66 % (fasting sample) and 27 % (post prandial sample) and ammonia from 60 % of the patients. Laboratory findings and their usefulness in diagnostics of different hepatobiliary diseases in dogs and cats were in agreement with what is described by scientific reports. 86.3 % of the patients had been studied with ultrasound, which was often useful, especially as a way to support the diagnosis. The most used biopsy method was fine-needle cytology which was taken from 93 patients,…
Subjects/Keywords: dog; cat; liver disease; liver biopsy; bile acid; ammonia; laparoscopy; laparotomy; Pieneläinten sisätaudit; Smådjurs internmedicin; Small Animal Internal Medicine; dog; cat; liver disease; liver biopsy; bile acid; ammonia; laparoscopy; laparotomy
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APA ·
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MLA ·
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APA (6th Edition):
Apalkova, I. (2013). Prevalence and Diagnosis of Liver Diseases in Small Animal University Hospital 2007-2010 - a Retrospective Study. (Thesis). University of Helsinki. Retrieved from http://hdl.handle.net/10138/39611
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Apalkova, Irina. “Prevalence and Diagnosis of Liver Diseases in Small Animal University Hospital 2007-2010 - a Retrospective Study.” 2013. Thesis, University of Helsinki. Accessed March 08, 2021.
http://hdl.handle.net/10138/39611.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Apalkova, Irina. “Prevalence and Diagnosis of Liver Diseases in Small Animal University Hospital 2007-2010 - a Retrospective Study.” 2013. Web. 08 Mar 2021.
Vancouver:
Apalkova I. Prevalence and Diagnosis of Liver Diseases in Small Animal University Hospital 2007-2010 - a Retrospective Study. [Internet] [Thesis]. University of Helsinki; 2013. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/10138/39611.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Apalkova I. Prevalence and Diagnosis of Liver Diseases in Small Animal University Hospital 2007-2010 - a Retrospective Study. [Thesis]. University of Helsinki; 2013. Available from: http://hdl.handle.net/10138/39611
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
UCLA
28.
Lee, Jessica Mei-Ping.
The Role of Diet1 in Bile Acid Metabolism.
Degree: Human Genetics, 2013, UCLA
URL: http://www.escholarship.org/uc/item/2kt1v5r1
► Elevated cholesterol levels are associated with increased risk for atherosclerosis, heart disease and stroke. Variations in plasma cholesterol levels among individuals are determined by the…
(more)
▼ Elevated cholesterol levels are associated with increased risk for atherosclerosis, heart disease and stroke. Variations in plasma cholesterol levels among individuals are determined by the interaction of environmental and genetic factors, many of which remain to be identified. This dissertation presents the initial characterization of a novel gene Diet1, the product of which influences plasma cholesterol levels through its effects on bile acid metabolism. Bile acids are synthesized from cholesterol in the liver, and secreted into the small intestine to aid in digestion. At the terminal end of the small intestine, bile acid are actively reabsorbed and sent to the liver (reviewed in Chapter 1).Preceding my studies, a mutation in Diet1 was identified as the underlying basis for resistance to diet-induced hypercholesterolemia and atherosclerosis in the C57BL/6ByJ inbred mouse strain. My studies have characterized the physiological and cellular function of Diet1, a large modular protein consisting of repeating LDL receptor A2 and MAM (meprin-A5-receptor protein tyrosine phosphatase mu) domains. Diet1 expression is restricted to the small intestine and the kidney cortex. We determined that Diet1 influences the communication from small intestine to liver to modulate the rate at which bile acids are synthesized (Chapter 2). Specifically, Diet1 affects production of the intestinal hormone fibroblast growth factor 15/19 (FGF15/19), which travels through the enterohepatic circulation and activates hepatic receptors, leading to down-regulation of bile acid synthesis. The absence of this regulation in Diet1-deficient mice explains the unregulated conversion of cholesterol to bile acids and protection from hypercholesterolemia that is observed in these animals.The identification of Diet1 as a determinant of FGF15/19 and bile acid levels in the mouse led to the hypothesis that genetic variation in DIET1 may influence variations in plasma bile acid levels in the human population, and may also underlie disease states characterized by aberrant bile acid levels. To explore these possibilities we resequenced DIET1 in individuals affected with type 2 chronic bile acid diarrhea (Chapter 3), and in individuals from a small Mexican population sample that have extremely high or low bile acid levels (Chapter 4). These studies led to the identification of a common DIET1 nonsynonymous polymorphism that influences the levels of FGF19 secreted from cultured human cells, and an enrichment of rare nonsynonymous DIET1 variants in the individuals with extreme low bile acid levels. Our studies establish Diet1 as a regulator of hepatic bile acid synthesis through its effect on the production of the intestinal hormone FGF15/19. They also implicate genetic variation in DIET1 as a determinant of human FGF19 and bile acid levels.
Subjects/Keywords: Genetics; Molecular biology; bile acid; Diet1; enterohepatic circulation; feedback regulation; FGF15/19; nonsynonymous variant
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APA (6th Edition):
Lee, J. M. (2013). The Role of Diet1 in Bile Acid Metabolism. (Thesis). UCLA. Retrieved from http://www.escholarship.org/uc/item/2kt1v5r1
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Lee, Jessica Mei-Ping. “The Role of Diet1 in Bile Acid Metabolism.” 2013. Thesis, UCLA. Accessed March 08, 2021.
http://www.escholarship.org/uc/item/2kt1v5r1.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Lee, Jessica Mei-Ping. “The Role of Diet1 in Bile Acid Metabolism.” 2013. Web. 08 Mar 2021.
Vancouver:
Lee JM. The Role of Diet1 in Bile Acid Metabolism. [Internet] [Thesis]. UCLA; 2013. [cited 2021 Mar 08].
Available from: http://www.escholarship.org/uc/item/2kt1v5r1.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Lee JM. The Role of Diet1 in Bile Acid Metabolism. [Thesis]. UCLA; 2013. Available from: http://www.escholarship.org/uc/item/2kt1v5r1
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Illinois – Urbana-Champaign
29.
Ryerson, Daniel E.
Role of Src phosphorylation of FXR in bile acid regulation.
Degree: PhD, Molecular & Integrative Physi, 2017, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/97590
► Bile acids are physiological detergents which aid in the absorption of dietary lipids and lipid soluble vitamins but also function as fed state signaling molecules.…
(more)
▼ Bile acids are physiological detergents which aid in the absorption of dietary lipids and lipid soluble vitamins but also function as fed state signaling molecules. Elevated
bile acid levels in the liver can lead to cholestatic injury, primary biliary cirrhosis, fibrosis, and liver cancer; therefore, these levels must be tightly regulated. The farnesoid X receptor (FXR) is the primary
bile acid nuclear receptor and acts as the master regulator of
bile acid homeostasis, preventing liver damage caused by
bile acid accumulation. FXR does this by regulating the expression of many target genes in the gut and liver including the intestinal hormone fibroblast growth factor 19 (FGF19) and orphan nuclear receptor small heterodimer partner (SHP). In response to elevated hepatic
bile acid levels FXR, acting directly as well as through FGF19 and SHP, inhibits the synthesis of
bile acids, downregulates
bile acid importers, upregulates
bile acid exporters along with genes involved in
bile acid conjugation and detoxification. These important roles of FXR are highlighted by the phenotypic effects observed in FXR knockout (FXR-/-) mice. FXR-/- mice display elevated
bile acid pool size as well as elevated serum
bile acid levels. Additionally, FXR-/- mice show signs of liver damage and develop spontaneous tumors as they age. Understanding how FXR receives signals and translates them into transcriptional responses to mediate these diverse cellular effects will be important for the development of therapeutic agents to treat cholestatic liver disorders.
One mechanism through which FXR activity is regulated is signal-induced post-translational modifications. FXR has been shown to undergo multiple types of post-translational modifications including phosphorylation, methylation, acetylation and sumoylation in response to physiological and pathological signals. These modifications affect FXR in many ways including modulating subcellular localization, stability, DNA binding, interaction with transcriptional coregulators and affecting the expression of FXR target genes in a gene selective manner. Mutation of a single amino
acid, disrupting one of these post-translational modifications, has been shown to dramatically alter FXR function. Interestingly, some of these post-translational modifications have been shown to be misregulated in models of disease, which highlights the importance of understanding the molecular mechanisms through which FXR is post-translationally modified.
In this study a new post-translational modification of FXR was identified which profoundly impacts FXR transcriptional activity. Unbiased mass spectrometry based proteomic analysis showed that tyrosine-67 of FXR is rapidly phosphorylated in liver hepatocytes in response to treatment with either natural
bile acids or FGF19. Biochemical analysis paired with bioinformatic tools identified Src as the kinase responsible for this post-translational modification. Feeding mice a diet supplemented with the primary
bile acid cholic
acid (CA) led to interaction…
Advisors/Committee Members: Kemper, Jongsook K (advisor), Kemper, Jongsook K (Committee Chair), Shapiro, David (committee member), Raetzman, Lori (committee member), Anakk, Sayeepriyadarshini (committee member).
Subjects/Keywords: Bile acid; Fibroblast growth factor 19 (FGF19); Farnesoid X receptor (FXR); Src; Phosphorylation
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APA (6th Edition):
Ryerson, D. E. (2017). Role of Src phosphorylation of FXR in bile acid regulation. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/97590
Chicago Manual of Style (16th Edition):
Ryerson, Daniel E. “Role of Src phosphorylation of FXR in bile acid regulation.” 2017. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed March 08, 2021.
http://hdl.handle.net/2142/97590.
MLA Handbook (7th Edition):
Ryerson, Daniel E. “Role of Src phosphorylation of FXR in bile acid regulation.” 2017. Web. 08 Mar 2021.
Vancouver:
Ryerson DE. Role of Src phosphorylation of FXR in bile acid regulation. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2017. [cited 2021 Mar 08].
Available from: http://hdl.handle.net/2142/97590.
Council of Science Editors:
Ryerson DE. Role of Src phosphorylation of FXR in bile acid regulation. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2017. Available from: http://hdl.handle.net/2142/97590
Indian Institute of Science
30.
Chakrabarty, Arkajyoti.
Bile Acid Based Supramolecular Gels, Semiconductor Nanocrystals And Soft Hybrid Materials.
Degree: PhD, Faculty of Science, 2016, Indian Institute of Science
URL: http://etd.iisc.ac.in/handle/2005/2513
► Chapter 1. General Introduction This chapter gives an introduction to supramolecular organo/hydrogels and the related bile acid chemistry touching upon the gelation properties of the…
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▼ Chapter 1. General Introduction
This chapter gives an introduction to supramolecular organo/hydrogels and the related
bile acid chemistry touching upon the gelation properties of the
bile acid derivatives. Diverse applications of the supramolecular gels are illustrated with several examples. In the concluding section of this chapter, a brief introduction on the semiconductor nanocrystals is provided. Finally, the content of the thesis is outlined.
Chapter 2.
Bile Acid Derived Novel Organo/hydrogelators
Part 1.
Bile Acid Derived Organo/hydrogelators With a Basic Side Chain
Cationic analogues of
bile acids which showed remarkable gelation properties in water were reported from our laboratory. This led us to investigate the aggregation behaviour of some of the lithocholic and deoxycholic
acid derivatives having a basic side-chain.
Figure 1.
Bile acid based organo/hydrogelators containing a basic side-chain.
In this part, an organogelator 1 and a hydrogelator 2 derived from parent
bile acids have been described with respect to their gelation properties, morphology, thermal and mechanical stability of the gels. The organo/hydrogels were shown to be responsive to
acid-base stimuli as the organogel formed only in the protonated state and the hydrogel formed in the neutral form of the tertiary amines. The xerogel fibres obtained from the organogel were found to be solid-like and stable up to 200 °C as confirmed by variable temperature polarizing optical microscopy. The non-fluorescent organogel was doped with a fluorescent dye (coumarin 153) to design a novel dye-organogel composite material which was investigated with laser scanning confocal fluorescence microscopy showing the dye molecules were uniformly deposited on the organogel fibres.
Part 2. Serendipitous Organogelation by Dimeric
Bile Acid Esters
This section highlights our work on the organogelators based on a number of dimeric esters consisting of different
bile acid units.
Figure 2. The three different dimeric
bile acid esters as organogelators.
In this part, three
bile acid derived dimeric esters (1, 2 and 3) were shown to possess organogelation properties in aromatic and halogenated aromatic solvents. We studied the morphological features and rheological properties of these organogels. Next, the organogel matrix was exploited to generate and stabilize gold nanoparticles and prepare AuNP/gel hybrid material.
Chapter 3. Cholate Hydrogels and Soft Gel-nanoparticle Hybrid Materials
Sodium cholate does not form gel in water under any condition as compared to other sodium salts of other
bile acids such as sodium deoxycholate and lithocholate which show pH-dependent gelation behaviour.
Figure 3. Metal cholate hydrogels derived from sodium cholate and a variety of metal ions.
In this chapter, super hydrogelation of sodium cholate induced by a variety of metal ions (Ca2+, Cu2+, Co2+, Zn2+, Cd2+, Hg2+ and Ag+) is highlighted with respect to their morphology and mechanical strength/stability. The calcium cholate supramolecular system showed the…
Advisors/Committee Members: Maitra, Uday (advisor).
Subjects/Keywords: Bile Acid Supramolecular Gels; Semiconductor Nanocrystals; Supramolecular Organogels; Bile Acid Chemistry; Organogelation; Dimeric Bile Acid Esters; Organogelators; Cholate Hydrogels; Soft Gel-Nanoparticle Hybrid Materials; Cadium Deoxycholate; Cadium Selenide Nanocrystals; Soft Hybrid Materials; Supramolecular Hydrogels; Hydrogelators; Hydrogels; Supramolecular Gels; CdSe Nanocrystals; Hydrogelation; Organic Chemistry
Record Details
Similar Records
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Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Chakrabarty, A. (2016). Bile Acid Based Supramolecular Gels, Semiconductor Nanocrystals And Soft Hybrid Materials. (Doctoral Dissertation). Indian Institute of Science. Retrieved from http://etd.iisc.ac.in/handle/2005/2513
Chicago Manual of Style (16th Edition):
Chakrabarty, Arkajyoti. “Bile Acid Based Supramolecular Gels, Semiconductor Nanocrystals And Soft Hybrid Materials.” 2016. Doctoral Dissertation, Indian Institute of Science. Accessed March 08, 2021.
http://etd.iisc.ac.in/handle/2005/2513.
MLA Handbook (7th Edition):
Chakrabarty, Arkajyoti. “Bile Acid Based Supramolecular Gels, Semiconductor Nanocrystals And Soft Hybrid Materials.” 2016. Web. 08 Mar 2021.
Vancouver:
Chakrabarty A. Bile Acid Based Supramolecular Gels, Semiconductor Nanocrystals And Soft Hybrid Materials. [Internet] [Doctoral dissertation]. Indian Institute of Science; 2016. [cited 2021 Mar 08].
Available from: http://etd.iisc.ac.in/handle/2005/2513.
Council of Science Editors:
Chakrabarty A. Bile Acid Based Supramolecular Gels, Semiconductor Nanocrystals And Soft Hybrid Materials. [Doctoral Dissertation]. Indian Institute of Science; 2016. Available from: http://etd.iisc.ac.in/handle/2005/2513
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Open Access Theses and Dissertations