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You searched for subject:( VIGS). Showing records 1 – 3 of 3 total matches.

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The Ohio State University

1. Stasko, Anna K. Functional Gene Analysis of Resistance QTL towards <i>Phytophthora sojae</i> on SoybeanChromosome 19.

Degree: PhD, Plant Pathology, 2018, The Ohio State University

<i>Phytophthora sojae</i> is the causal agent of Phytophthora root and stem rot of soybean. One of the most effective disease management strategies against this pathogen is the use of resistant cultivars, primarily through single gene, <i>Rps</i>-mediated resistance. However, numerous populations of <i>P. sojae</i> have adapted to most <i>Rps</i> genes that are deployed in modern soybean cultivars, rendering them susceptible to this pathogen. Quantitative resistance, conferred by quantitative disease resistance loci (QDRL), offers an alternative to <i>Rps</i>-based resistance. Previous studies mapped two QDRL to chromosome 19 in the soybean cultivar Conrad, which has a high level of quantitative resistance. A recombinant inbred line (RIL) population derived from a cross of Conrad by Sloan (a moderately susceptible cultivar) used for mapping these QDRL was advanced to the F9:11 generation. This population was used to map/re-map the QDRL towards three isolates of <i>P. sojae</i>, and one isolate each of <i>Pythium irregulare</i> and <i>Fusarium graminearum</i>, using the SoySNP6K BeadChip for high-density marker genotyping. A total of ten, two, and three QDRL and suggestive QDRL were found that confer resistance to <i>P. sojae, Py. irregulare,</i> and <i>F. graminearum</i>, respectively. Individual QDRL explained 2-13.6% of the phenotypic variance (PV). One QDRL for both <i>Py. irregulare</i> and <i>F. graminearum</i> co-localized on chromosome 19. This resistance was contributed by Sloan and was juxtaposed to a QDRL for <i>P. sojae</i> with resistance contributed from Conrad. Alleles for resistance to different pathogens contributed from different parents in the same region, the number of unique QDRL for each pathogen, and the lack of correlation of resistance suggest that different mechanisms are involved in resistance towards these three pathogens. Interestingly, the QDRL located on chromosome 19 contained several genes related to auxin processes, which are known to contribute to susceptibility to several pathogens in <i>Arabidopsis</i> and may contribute to susceptibility of soybean to <i>P. sojae</i>. In this study, auxin metabolites were measured in <i>P. sojae</i> mycelia, media from <i>P. sojae</i> liquid cultures, and inoculated soybean roots. Auxin precursors were detected in the mycelia of <i>P. sojae</i> as well as the synthetic media. More importantly, auxin levels were significantly higher in inoculated roots than the mock controls in both resistant and susceptible genotypes at 48 hours after inoculation (hai). To examine the role of auxin transport in susceptibility to <i>P. sojae</i>, the nucleotide sequences and expression of root-related soybean auxin efflux transporters, <i>GmPINs</i>, were compared between Conrad and Sloan. There were sequence differences between the two cultivars; however, experimental variability prevented accurate detection of expression differences through a quantitative PCR approach. An auxin transport inhibitor and a synthetic auxin were applied to Conrad and Sloan… Advisors/Committee Members: Dorrance, Anne (Advisor).

Subjects/Keywords: Plant Pathology; Phytophora sojae; soybean; quantitative resistance; auxin; high-density mapping; RNAi; VIGS

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Stasko, A. K. (2018). Functional Gene Analysis of Resistance QTL towards <i>Phytophthora sojae</i> on SoybeanChromosome 19. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1524139406566913

Chicago Manual of Style (16th Edition):

Stasko, Anna K. “Functional Gene Analysis of Resistance QTL towards <i>Phytophthora sojae</i> on SoybeanChromosome 19.” 2018. Doctoral Dissertation, The Ohio State University. Accessed October 22, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1524139406566913.

MLA Handbook (7th Edition):

Stasko, Anna K. “Functional Gene Analysis of Resistance QTL towards <i>Phytophthora sojae</i> on SoybeanChromosome 19.” 2018. Web. 22 Oct 2019.

Vancouver:

Stasko AK. Functional Gene Analysis of Resistance QTL towards <i>Phytophthora sojae</i> on SoybeanChromosome 19. [Internet] [Doctoral dissertation]. The Ohio State University; 2018. [cited 2019 Oct 22]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1524139406566913.

Council of Science Editors:

Stasko AK. Functional Gene Analysis of Resistance QTL towards <i>Phytophthora sojae</i> on SoybeanChromosome 19. [Doctoral Dissertation]. The Ohio State University; 2018. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1524139406566913

2. Gama, Florinda Maria Martins. Movimento e sinalização do ferro em plantas com estratégia I.

Degree: 2018, RCAAP

A deficiência de Fe provocou decréscimos no teor de clorofila total, acréscimos na atividade da QFR, alterações morfológicas a nível radicular, diminuição de absorção e translocação de Fe nos diferentes órgãos. No morangueiro o decréscimo do Fe incidiu nas raízes, na coroa e nas flores. No Poncirus o teor de Fe diminuiu nas raízes. Como alternativa à absorção de Fe ocorreu uma acumulação dos metais Zn, Mn e Cu especialmente nas raízes e folhas novas. No morangueiro os compostos orgânicos acumularam nas folhas velhas e jovens e diminuíram nas raízes. O pulso de Fe fornecido na solução nutritiva a plantas cloróticas de morangueiro levou à recuperação da deficiência em 12 dias. A partição de Fe foi direcionada para a coroa e para as flores, associado a acumulação de ácidos sucínico e cítrico ao nível radicular e permitiu maior absorção de Fe. Foram estudadas as alterações no mecanismo de resposta em plantas com o gene FRO1 silenciado. Em plantas silenciadas a deficiência de Fe levou à redução da expressão do gene, decréscimo da atividade QFR e diminuição na síntese de clorofila. Em Nicotiana o padrão de distribuição do Fe entre folhas velhas e jovens foi alterado e não foi possível recuperar as plantas silenciadas, as plantas não conseguiram retomar o metabolismo na síntese de clorofila nem ativar a enzima QFR. Foi estudado o comportamento de porta-enxertos de Poncirus com a estratégia redutora e as adaptações fisiológica ativas quando transplantadas para solo calcário. Estas plantas apresentaram desempenho semelhante em relação às plantas que cresceram numa primeira fase sem limitação de Fe indicando que os mecanismos de adaptação e o aumento nutricional induzidos pela deficiência de Fe podem ser utilizados como uma ferramenta de defesa que contribuirá para as plantas tolerarem melhor as diferentes restrições do solo em condições de campo.

Fe deficiency caused decreases in total chlorophyll, increases in ferric chelate reductase activity, morphological alterations at the root level and decreases in Fe absorption and translocation in different organs. In strawberry plants, Fe content decreased in the roots, the crown and the flowers. In Poncirus, the Fe content decreased in the roots. As an alternative to Fe absorption, an accumulation of Zn, Mn and Cu metals occurred especially in roots and young leaves. In strawberry organic compounds accumulated in mature and young leaves and lowered in the roots. The Fe pulse added to the nutrient solution of chlorotic strawberry plants led to Fe deficiency recovery after 12 days. Fe partitioning was directed to the crown and flowers, associated with the accumulation of succinic and citric acids at the root level, which allowed higher Fe absorption. Changes to the response mechanism in plants with the FRO1 gene silenced were studied. In silenced plants, Fe deficiency led to reduced gene expression, decreased FCR activity and decreased chlorophyll synthesis. In Nicotiana, the pattern of Fe distribution between mature and young leaves was altered and silenced plants were unable to…

Advisors/Committee Members: Correia, Maribela Pestana, Nolasco, Gustavo.

Subjects/Keywords: Deficiência de ferro; Quelato de ferro redutase; Solo calcário; VIGS (Virus induced gene silencing).; Nutrientes; Domínio/Área Científica::Ciências Naturais::Ciências da Terra e do Ambiente

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Gama, F. M. M. (2018). Movimento e sinalização do ferro em plantas com estratégia I. (Thesis). RCAAP. Retrieved from https://www.rcaap.pt/detail.jsp?id=oai:sapientia.ualg.pt:10400.1/10822

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Gama, Florinda Maria Martins. “Movimento e sinalização do ferro em plantas com estratégia I.” 2018. Thesis, RCAAP. Accessed October 22, 2019. https://www.rcaap.pt/detail.jsp?id=oai:sapientia.ualg.pt:10400.1/10822.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Gama, Florinda Maria Martins. “Movimento e sinalização do ferro em plantas com estratégia I.” 2018. Web. 22 Oct 2019.

Vancouver:

Gama FMM. Movimento e sinalização do ferro em plantas com estratégia I. [Internet] [Thesis]. RCAAP; 2018. [cited 2019 Oct 22]. Available from: https://www.rcaap.pt/detail.jsp?id=oai:sapientia.ualg.pt:10400.1/10822.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gama FMM. Movimento e sinalização do ferro em plantas com estratégia I. [Thesis]. RCAAP; 2018. Available from: https://www.rcaap.pt/detail.jsp?id=oai:sapientia.ualg.pt:10400.1/10822

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


The Ohio State University

3. Gedling, Cassidy Renee. Mechanisms of Resistance and Candidate Gene Analysis towards <i> Fusarium graminearum </i> and <i> Phytophthora sojae </i> in Soybean.

Degree: PhD, Plant Pathology, 2018, The Ohio State University

Numerous diseases affect soybean [<i>Glycine max</i> (L.) Merr] yields throughout the growing season in Ohio. Two soil borne pathogens <i> Fusarium graminearum </i> and <i> Phytophthora sojae </i> are known to reduce stand and yield. Currently, fungicide seed treatments are used to manage these pathogens, however, host plant resistance is often the best management strategy for field crops. Thus, the overall objective of the five chapters this dissertation was to identify mechanisms and candidate genes of resistance that are effective towards seed, seedling, and root rots caused by <i> Fusarium graminearum </i> and <i> P. sojae </i> in soybean. Quantitative disease resistance loci (QDRL) have been mapped in two separate recombinant inbred line (RIL) populations for resistance to <i> Fusarium graminearum </i>. In the F7:8 RIL derived from a cross Magellan X PI 567516C, one major QDRL was mapped. Fine mapping of this region identified four putative candidate genes for resistance to <i> Fusarium graminearum </i>. In an additional population of Wyandot x PI 567301B, a major and minor QDRL was mapped to chromosome 8 and 6, respectively. Hybrid genome assembly, fine mapping, and RNA sequencing analysis narrowed the major QDRL to 2.5 cM containing three putative candidate genes for resistance or susceptibility. To validate these candidate genes functional analysis needs to be assessed at the seed level. To achieve this we modified the Apple latent spherical virus (ASLV) which allowed for direct inoculation of VIGS-triggering ALSV agro-infiltrated Nicotiana benthamiana leaves onto soybean unifoliates. However, this method is genotype dependent; the virus is detected in numerous reproductive structures including pods, embryos, stems, leaves, and roots. The last objective of this dissertation focuses on mechanisms of partial resistance to <i> Phytophthora sojae </i>. This oomycete is a leading pathogen of soybean, causing root and stem rot (PRR) across the North Central Region in the U.S. Twenty phenotypic quantitative trait loci (pQTL) were previously mapped in a F9:11 Conrad x Sloan recombinant inbred line (RIL) population on chromosomes 1, 4, 9, 15, 16, 18, and 19; however, these regions encompass large portions of the genome. Thus a systems genetics approach that incorporates expression QTL (eQTL) mapping, functional genomics, and gene co-expression analysis was taken to identify molecular mechanisms contributing towards partial resistance, with the specific objective of reducing the list of candidate genes potentially underpinning pQTL. A greater number of eQTL were mapped in inoculated samples relative to mock, indicating transcriptional reprogramming due to <i> P. sojae </i> infection. Of the six co-expression modules identified, three were related to PRR susceptibility driven by three casual hotspots. GO enrichment of casual hotspot GM_17_D indicates that cell wall modification is a putative mechanism for <i> P. sojae </i> resistance. A total of four eQTL and one eQTL hotspots were found to be… Advisors/Committee Members: Dorrance, Anne (Advisor).

Subjects/Keywords: Plant Pathology; Plant Biology; Plant Sciences; Genetics; eQTL; QTL; WGCNA; hybrid genome assembly; VIGS; ALSV; QDRL; partial resistance; quantitative resistance; systems genetics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Gedling, C. R. (2018). Mechanisms of Resistance and Candidate Gene Analysis towards <i> Fusarium graminearum </i> and <i> Phytophthora sojae </i> in Soybean. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1524160566193078

Chicago Manual of Style (16th Edition):

Gedling, Cassidy Renee. “Mechanisms of Resistance and Candidate Gene Analysis towards <i> Fusarium graminearum </i> and <i> Phytophthora sojae </i> in Soybean.” 2018. Doctoral Dissertation, The Ohio State University. Accessed October 22, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1524160566193078.

MLA Handbook (7th Edition):

Gedling, Cassidy Renee. “Mechanisms of Resistance and Candidate Gene Analysis towards <i> Fusarium graminearum </i> and <i> Phytophthora sojae </i> in Soybean.” 2018. Web. 22 Oct 2019.

Vancouver:

Gedling CR. Mechanisms of Resistance and Candidate Gene Analysis towards <i> Fusarium graminearum </i> and <i> Phytophthora sojae </i> in Soybean. [Internet] [Doctoral dissertation]. The Ohio State University; 2018. [cited 2019 Oct 22]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1524160566193078.

Council of Science Editors:

Gedling CR. Mechanisms of Resistance and Candidate Gene Analysis towards <i> Fusarium graminearum </i> and <i> Phytophthora sojae </i> in Soybean. [Doctoral Dissertation]. The Ohio State University; 2018. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1524160566193078

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