You searched for subject:( SUMO).
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1.
Uzoma, Ijeoma K.
GLOBAL ANALYSIS OF SUMO E3 LIGASE SPECIFICITY UNCOVERS CROSSTALK-MEDIATED KINASE ACTIVATION.
Degree: 2014, Johns Hopkins University
URL: http://jhir.library.jhu.edu/handle/1774.2/37923 
► Over 100 kinases were recovered as SUMO substrates in our assays which points to a global phenomenon of kinase regulation by SUMO modification. The studies…
(more)
▼ Over 100 kinases were recovered as
SUMO substrates in our assays which points to a global phenomenon of kinase regulation by
SUMO modification. The studies presented in chapter 4 describe our efforts to validate and characterize
SUMO modification of focal adhesion, tyrosine kinase, Pyk2. Here, we demonstrate that SUMOylation of Pyk2 is a novel PTM that serves to amplify intrinsic kinase activity by enhancing autophosphorylation. Further biochemical studies reveal SUMOylation of Pyk2 enhances its association with putative binding protein Src kinase, promotes phosphorylation of downstream focal adhesion protein paxillin, and mediates ERK activation. These findings led us to examine the role of SUMOylation of Pyk2 in cell migration. Our results suggest SUMOylation of Pyk2 amplifies its promigratory function in MDA-MB-231 breast cancer cells. These studies have revealed a novel mechanism for Pyk2 activation, regulated by crosstalk between phosphorylation and SUMOylation.
Collectively, we have illuminated the connections between SUMOylated kinases along a particular signaling axis, promoting enzyme activity, protein interactions, and activation of numerous nodes in a pathway. We believe that the work presented in this thesis will have a profound impact on the studies of SUMOylation and its crosstalk with other PTMs in a broad range of biological processes.
Advisors/Committee Members: Wolberger, Cynthia (advisor).
Subjects/Keywords: Proteomics; SUMO
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APA (6th Edition):
Uzoma, I. K. (2014). GLOBAL ANALYSIS OF SUMO E3 LIGASE SPECIFICITY UNCOVERS CROSSTALK-MEDIATED KINASE ACTIVATION. (Thesis). Johns Hopkins University. Retrieved from http://jhir.library.jhu.edu/handle/1774.2/37923
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Uzoma, Ijeoma K. “GLOBAL ANALYSIS OF SUMO E3 LIGASE SPECIFICITY UNCOVERS CROSSTALK-MEDIATED KINASE ACTIVATION.” 2014. Thesis, Johns Hopkins University. Accessed January 18, 2021.
http://jhir.library.jhu.edu/handle/1774.2/37923.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Uzoma, Ijeoma K. “GLOBAL ANALYSIS OF SUMO E3 LIGASE SPECIFICITY UNCOVERS CROSSTALK-MEDIATED KINASE ACTIVATION.” 2014. Web. 18 Jan 2021.
Vancouver:
Uzoma IK. GLOBAL ANALYSIS OF SUMO E3 LIGASE SPECIFICITY UNCOVERS CROSSTALK-MEDIATED KINASE ACTIVATION. [Internet] [Thesis]. Johns Hopkins University; 2014. [cited 2021 Jan 18].
Available from: http://jhir.library.jhu.edu/handle/1774.2/37923.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Uzoma IK. GLOBAL ANALYSIS OF SUMO E3 LIGASE SPECIFICITY UNCOVERS CROSSTALK-MEDIATED KINASE ACTIVATION. [Thesis]. Johns Hopkins University; 2014. Available from: http://jhir.library.jhu.edu/handle/1774.2/37923
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
2.
Lin, Tse-yu.
Investigation of the SUMO-interacting motif of p38.
Degree: Master, Institute of Biomedical Sciences, 2015, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0721115-145645
► SUMOylation is a posttranslational modification by the small ubiquitin-related modifier (SUMO), SUMOylation plays important roles in the regulation of diverse cellular processes. SUMO can interact…
(more)
▼ SUMOylation is a posttranslational modification by the small ubiquitin-related modifier (
SUMO), SUMOylation plays important roles in the regulation of diverse cellular processes.
SUMO can interact non-covalently with proteins through the
SUMO-interacting motif (SIM). The p38 mitogen-activated protein kinases are a class of mitogen-activated protein kinases that are responsive to stress stimuli. The phosphorylation-dependent nuclear translocation of p38 is a common phenomenon when cells are stimulated with various stresses. Our previous studies demonstrated that overexpressing RFP-
SUMO-1/2 caused endogenous p-p38 co-localized with RFP-
SUMO-1/2 in nuclear. Our previous studies also demonstrated that p38 cannot be SUMOylated, but it can interact with
SUMO-1/2 in yeast two-hybrid. We speculated that p38 has SIM. In this study, we focused on identification of the SIM of p38 and whether the SIM regulates the nuclear translocation of p38 and p38-mediated apoptosis. In immunocytochemistry, we found that wild type p38 and p-p38 co-localized with RFP-
SUMO-1/2 in nuclear. Single SIM mutant (SIM-1*, SIM-2* and SIM-3*) p38 and p-p38 also co-localized with RFP-
SUMO-1/2 in nuclear. In pull-down assay, results indicated that SIM-3 was essential for
SUMO-interacting of p38, SIM-1 and SIM-2 also involved in
SUMO-interacting of p38, and the interacting through SIM-3 was stronger. Interacting of
SUMO-2 to p38 was stronger than interacting of
SUMO-1 to p38. In MTT assay, overexpression of wild type p38 and
SUMO-1/2 decreased cell survival rate of AGS cells. SIM-1*, SIM-2* and SIM-3* all inhibited the decreases of the cell survival rate caused by the overexpression of p38 and
SUMO-1/2. We found that p38 can interact with
SUMO-1/2 through the SIM, and SIM mediates the p38-mediated apoptosis of AGS cells.
Advisors/Committee Members: Hung-Wen Huamg (chair), Ping-I Hsu (chair), Angela Chen (committee member).
Subjects/Keywords: p38; SUMO-interacting motif; non-covalent interaction; SUMO-1; SUMO-2
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APA (6th Edition):
Lin, T. (2015). Investigation of the SUMO-interacting motif of p38. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0721115-145645
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Lin, Tse-yu. “Investigation of the SUMO-interacting motif of p38.” 2015. Thesis, NSYSU. Accessed January 18, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0721115-145645.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Lin, Tse-yu. “Investigation of the SUMO-interacting motif of p38.” 2015. Web. 18 Jan 2021.
Vancouver:
Lin T. Investigation of the SUMO-interacting motif of p38. [Internet] [Thesis]. NSYSU; 2015. [cited 2021 Jan 18].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0721115-145645.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Lin T. Investigation of the SUMO-interacting motif of p38. [Thesis]. NSYSU; 2015. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0721115-145645
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Alberta
3.
Beatty, Jessica.
Examining the Potential Modification of the Protein Tyrosine
Kinase Pyk2 by SUMO-1.
Degree: MS, Department of Medical Microbiology and
Immunology, 2013, University of Alberta
URL: https://era.library.ualberta.ca/files/sf2685977
► Proline-rich tyrosine kinase 2 (Pyk2) is a non-receptor protein tyrosine kinase that is highly expressed in hematopoietic cells. Our lab generated two polyclonal antibodies to…
(more)
▼ Proline-rich tyrosine kinase 2 (Pyk2) is a
non-receptor protein tyrosine kinase that is highly expressed in
hematopoietic cells. Our lab generated two polyclonal antibodies to
investigate the regulation of Pyk2 in macrophages and T cells. In
macrophages the N-terminal antibody immunoprecipitated a higher
molecular weight form of Pyk2. This shift was not due to
differential phosphorylation or isoform expression. Since FAK, a
close relative to Pyk2 undergoes a molecular weight shift due to
SUMOylation, my thesis project was to investigate the potential
SUMOylation of Pyk2. This study demonstrates that endogenous and
exogenous Pyk2 associates with SUMO-1. The E3 ligase PIAS1 was
shown to promote the association of Pyk2 with SUMO-1. Lysines 35,
145, and 646 were not the sites of Pyk2 SUMOylation, although
SUMO-1 does associate with Pyk2 in the FERM domain. Direct Pyk2
SUMOylation was not confirmed, although SUMO-1 and PIAS1
overexpression increases Pyk2 protein levels.
Subjects/Keywords: Pyk2; SUMO-1; PIAS1
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APA (6th Edition):
Beatty, J. (2013). Examining the Potential Modification of the Protein Tyrosine
Kinase Pyk2 by SUMO-1. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/sf2685977
Chicago Manual of Style (16th Edition):
Beatty, Jessica. “Examining the Potential Modification of the Protein Tyrosine
Kinase Pyk2 by SUMO-1.” 2013. Masters Thesis, University of Alberta. Accessed January 18, 2021.
https://era.library.ualberta.ca/files/sf2685977.
MLA Handbook (7th Edition):
Beatty, Jessica. “Examining the Potential Modification of the Protein Tyrosine
Kinase Pyk2 by SUMO-1.” 2013. Web. 18 Jan 2021.
Vancouver:
Beatty J. Examining the Potential Modification of the Protein Tyrosine
Kinase Pyk2 by SUMO-1. [Internet] [Masters thesis]. University of Alberta; 2013. [cited 2021 Jan 18].
Available from: https://era.library.ualberta.ca/files/sf2685977.
Council of Science Editors:
Beatty J. Examining the Potential Modification of the Protein Tyrosine
Kinase Pyk2 by SUMO-1. [Masters Thesis]. University of Alberta; 2013. Available from: https://era.library.ualberta.ca/files/sf2685977
University of Dundee
4.
Hands, Katherine J.
Defining the mechanism of arsenic-induced degradation of PML.
Degree: PhD, 2012, University of Dundee
URL: https://discovery.dundee.ac.uk/en/studentTheses/a5b14b03-25b0-457f-8dd9-9facf98b6415
;
https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.578872
► Arsenic trioxide is a clinically effective treatment for the disease acute promyelocytic leukaemia (APL) which is caused by the chromosomal translocation t(15;17) which fuses the…
(more)
▼ Arsenic trioxide is a clinically effective treatment for the disease acute promyelocytic leukaemia (APL) which is caused by the chromosomal translocation t(15;17) which fuses the promyelocytic leukaemia (PML) protein to the retinoic receptor alpha (RARa). The PML-RARa oncoprotein disrupts normal retinoic acid signalling and the function of PML nuclear bodies (PML-NBs), subnuclear protein complexes with roles in control of apoptosis and cellular senescence. Treatment with arsenic induces rapid post translational modification of PML and with the small ubiquitin like modifier (SUMO). SUMO modification of PML recruits the SUMO targeted ubiquitin E3 ligase RNF4 via four SUMO interaction motifs within the N-terminal region of RNF4. PML is then ubiquitylated and targeted for proteasomal degradation. In APL, these events trigger degradation of PML-RARa, curing the disease. To further investigate the process of arsenic induced degradation of PML, a high content siRNA screen was designed to monitor the fate of a YFP linked version of PML after siRNA mediated knockdown of components of the ubiquitin system and arsenic treatment. RNF4 depletion prior to arsenic treatment prevented PML degradation and resulted in accumulation of PML in large, bright PML-NBs. This was used as a positive control. A library of siRNAs targeting 1067 gene products were screened to identify those which perturbed the process of arsenic mediated degradation of PML, and those which affected the stability of PML in untreated cells. A number of putative hits were identified. Depletion of the cullin RING ligase scaffold CUL3, and the NEDD8 E3 ligase DCUN1D1 resulted in striking accumulation of PML, suggesting PML may be a substrate of a CUL3 RING ligase complex. Further experiments using the inhibitor of neddylation, MLN4924 support this hypothesis. PML is expressed as a various isoforms which encode a unique C-terminal region, due to alternative splicing. The second part of this study investigated the role of this variable C-terminal region in the response of the six major PML isoforms to arsenic treatment. Using a system in which only a single eYFP-linked PML isoform is expressed, differences in the localisation of PML isoforms following arsenic treatment were identified, with PML I, II and VI found to accumulate in the cytoplasm following arsenic treatment, whereas PML III, IV and V did not. A high content imaging assay identified PML V as the isoform most readily degraded following arsenic treatment, and PML IV as relatively resistant to degradation. Using siRNA it was demonstrated that arsenic induced degradation of all PML isoforms is dependent on the ubiquitin E3 ligase RNF4. Intriguingly, depletion of RNF4 resulted in marked accumulation of PML V, suggesting this isoform is an optimal substrate for RNF4. Thus the variable C-terminal domain influences the rate and location of degradation of PML isoforms following arsenic treatment.
Subjects/Keywords: 616.1; PML; Arsenic; SUMO
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APA (6th Edition):
Hands, K. J. (2012). Defining the mechanism of arsenic-induced degradation of PML. (Doctoral Dissertation). University of Dundee. Retrieved from https://discovery.dundee.ac.uk/en/studentTheses/a5b14b03-25b0-457f-8dd9-9facf98b6415 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.578872
Chicago Manual of Style (16th Edition):
Hands, Katherine J. “Defining the mechanism of arsenic-induced degradation of PML.” 2012. Doctoral Dissertation, University of Dundee. Accessed January 18, 2021.
https://discovery.dundee.ac.uk/en/studentTheses/a5b14b03-25b0-457f-8dd9-9facf98b6415 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.578872.
MLA Handbook (7th Edition):
Hands, Katherine J. “Defining the mechanism of arsenic-induced degradation of PML.” 2012. Web. 18 Jan 2021.
Vancouver:
Hands KJ. Defining the mechanism of arsenic-induced degradation of PML. [Internet] [Doctoral dissertation]. University of Dundee; 2012. [cited 2021 Jan 18].
Available from: https://discovery.dundee.ac.uk/en/studentTheses/a5b14b03-25b0-457f-8dd9-9facf98b6415 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.578872.
Council of Science Editors:
Hands KJ. Defining the mechanism of arsenic-induced degradation of PML. [Doctoral Dissertation]. University of Dundee; 2012. Available from: https://discovery.dundee.ac.uk/en/studentTheses/a5b14b03-25b0-457f-8dd9-9facf98b6415 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.578872
NSYSU
5.
Wang, Pin-Yao.
SUMOs mediate the nuclear transfer of p38 and p-p38 during Helicobacter pylori infection.
Degree: PhD, Institute of Biomedical Sciences, 2016, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0801116-023742
► p38 mitogen-activated protein kinase (MAPK) is of the essence in the cellâs response to environmental stresses, cytokines and DNA damage. p38 is distributed both in…
(more)
▼ p38 mitogen-activated protein kinase (MAPK) is of the essence in the cellâs response to environmental stresses, cytokines and DNA damage. p38 is distributed both in the cytosol and nucleus, and cytosolic p38 translocates into the nucleus in response to various stimuli, yet the exact mechanisms remain largely unclear. One response to cellular stress is the elevated expression of
SUMO proteins. SUMOs have many roles in cellular biology including promoting nuclear translocalization. In our study, we have demonstrated that exposure of human cells to Helicobacter pylori (Hp) induces the expression of SUMOs and the activation of p38. A non-covalent interaction between SUMOs and both non-phosphorylated and phosphorylated p38 (p38 and p-p38) was identified, and the interaction was found to be
SUMO concentration dependent. We found that upon Hp stimulation cytosolic p38 could be translocated into the nucleus by both
SUMO-1 and
SUMO-2, although both p38 and p-p38 have a stronger binding affinity for
SUMO-2 than for
SUMO-1. Mutation of
SUMO interacting motif 3 (SIM3) of p38 abolished its binding to SUMOs and decreased
SUMO-dependent nuclear transfer of p38. This study demonstrates that SUMOs serve as novel regulators of p38 and p-p38 nuclear translocation through a non-covalent
SUMO-p38 interaction, independent of the phosphorylation state of p38.
Advisors/Committee Members: Hurng-Werg Huang (chair), Yow-Ling Shiue (chair), Kuang-Hung Cheng (chair), Angela Chen (committee member), Ping-I Hsu (chair).
Subjects/Keywords: SUMO-1; SIM; Helicobacter pylori; p38; nuclear translocalization; SUMO-2
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APA ·
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APA (6th Edition):
Wang, P. (2016). SUMOs mediate the nuclear transfer of p38 and p-p38 during Helicobacter pylori infection. (Doctoral Dissertation). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0801116-023742
Chicago Manual of Style (16th Edition):
Wang, Pin-Yao. “SUMOs mediate the nuclear transfer of p38 and p-p38 during Helicobacter pylori infection.” 2016. Doctoral Dissertation, NSYSU. Accessed January 18, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0801116-023742.
MLA Handbook (7th Edition):
Wang, Pin-Yao. “SUMOs mediate the nuclear transfer of p38 and p-p38 during Helicobacter pylori infection.” 2016. Web. 18 Jan 2021.
Vancouver:
Wang P. SUMOs mediate the nuclear transfer of p38 and p-p38 during Helicobacter pylori infection. [Internet] [Doctoral dissertation]. NSYSU; 2016. [cited 2021 Jan 18].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0801116-023742.
Council of Science Editors:
Wang P. SUMOs mediate the nuclear transfer of p38 and p-p38 during Helicobacter pylori infection. [Doctoral Dissertation]. NSYSU; 2016. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0801116-023742
6.
Baik, Hayeon.
Role of the SUMO pathway in Acute Myeloid Leukemias response to treatments : Rôle de la sumoylation dans la réponse aux traitements des leucémies aiguës myéloïdes.
Degree: Docteur es, Biologie Santé, 2017, Montpellier
URL: http://www.theses.fr/2017MONTT016
► Les thérapies de différenciation sont une alternative prometteuse aux drogues génotoxiques utilisées en chimiothérapie pour le traitement de nombreux cancers. En particulier, l’acide tout-trans rétinoïque…
(more)
▼ Les thérapies de différenciation sont une alternative prometteuse aux drogues génotoxiques utilisées en chimiothérapie pour le traitement de nombreux cancers. En particulier, l’acide tout-trans rétinoïque (ATRA) est utilisé avec succès pour traiter la leucémie aiguë promyélocytaire, un sous-type des leucémies aiguës myéloïdes (LAM). Malheureusement, son efficacité clinique est limitée dans les autres sous-types des LAM. Cela est en particulier du à une répression épigénétique des gènes de réponse à l’ATRA. Les SUMO constituent une famille de modificateurs post-traductionnels apparentés à l’ubiquitine dont la conjugaison sur de nombreuses protéines, appelée sumoylation, est impliquée dans la régulation de nombreux processus cellulaires, dont la transcription. Dans ce contexte, l’objective de ma thèse a été de comprendre le rôle de la sumoylation dans la réponse des LAM aux thérapies de différenciation. Nous avons pu montrer que la sumoylation réprime la différenciation induite par ATRA dans plusieurs lignées cellulaires, des cellules primaires de patients y compris celles résistantes à la chimiothérapie. L’inhibition de la sumoylation par les inhibiteurs pharmacologiques ou la surexpression des désumoylases augmente de façon remarquable la différenciation par ATRA et, à l’inverse l’augmentation de la sumoylation suite à une surexpression de SUMO ou son enzyme de conjugaison Ubc9 réduit fortement l’efficacité d’ATRA. L’ATRA synergise avec l’inhibition de la sumoylation pour limiter la prolifération des cellules de LAM in vitro et in vivo. D’un point de vue mécanistique, l’inhibition de la sumoylation favorise la différenciation des cellules de LAM en facilitant l’expression des gènes responsables de la différenciation myéloïde. Ainsi, cibler la sumoylation constitue une approche prometteuse pour sensibiliser la LAM aux thérapies de différenciation.
Differentiation therapies are a promising alternative to genotoxic-based chemotherapies in the treatment of many cancers. In particular, All-trans-retinoic acid (ATRA) is successfully used for Acute Promyelocytic Leukemias, a subtype of Acute Myeloid Leukemias. However, its clinical efficiency is very limited in the other AML subtypes, in particular because of epigenetic repression of ATRA-responsive genes. SUMOs are a family of post-translational modifiers related to ubiquitin and their conjugation, sumoylation, to their substrate proteins regulate many processes including gene transcription. The aim of my thesis was to understand the role of sumoylation in AML responses to treatments. I showed that sumoylation represses ATRA-induced differentiation in many AML cell lines and primary patient samples, including those resistant to chemotherapies. Inhibition of sumoylation with pharmacological inhibitors or overexpression of desumoylases markedly increased their differentiation by ATRA and increasing sumoylation by overexpression of SUMO or its conjugating enzyme Ubc9 strongly reduce ATRA efficiency. Inhibition of sumoylation synergize with ATRA to arrest AML cells…
Advisors/Committee Members: Piechaczyk, Marc (thesis director).
Subjects/Keywords: Sumo; Atra; Lam; Différenciation; Sumo; Atra; Aml; Differentiation
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APA ·
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MLA ·
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to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Baik, H. (2017). Role of the SUMO pathway in Acute Myeloid Leukemias response to treatments : Rôle de la sumoylation dans la réponse aux traitements des leucémies aiguës myéloïdes. (Doctoral Dissertation). Montpellier. Retrieved from http://www.theses.fr/2017MONTT016
Chicago Manual of Style (16th Edition):
Baik, Hayeon. “Role of the SUMO pathway in Acute Myeloid Leukemias response to treatments : Rôle de la sumoylation dans la réponse aux traitements des leucémies aiguës myéloïdes.” 2017. Doctoral Dissertation, Montpellier. Accessed January 18, 2021.
http://www.theses.fr/2017MONTT016.
MLA Handbook (7th Edition):
Baik, Hayeon. “Role of the SUMO pathway in Acute Myeloid Leukemias response to treatments : Rôle de la sumoylation dans la réponse aux traitements des leucémies aiguës myéloïdes.” 2017. Web. 18 Jan 2021.
Vancouver:
Baik H. Role of the SUMO pathway in Acute Myeloid Leukemias response to treatments : Rôle de la sumoylation dans la réponse aux traitements des leucémies aiguës myéloïdes. [Internet] [Doctoral dissertation]. Montpellier; 2017. [cited 2021 Jan 18].
Available from: http://www.theses.fr/2017MONTT016.
Council of Science Editors:
Baik H. Role of the SUMO pathway in Acute Myeloid Leukemias response to treatments : Rôle de la sumoylation dans la réponse aux traitements des leucémies aiguës myéloïdes. [Doctoral Dissertation]. Montpellier; 2017. Available from: http://www.theses.fr/2017MONTT016
University of Dundee
7.
Tammsalu, Triin.
Global identification of SUMO modification sites.
Degree: PhD, 2016, University of Dundee
URL: https://discovery.dundee.ac.uk/en/studentTheses/5f8905a2-c31c-45ec-8173-4c91cf797342
;
https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.805988
► Post-translational modification by Small Ubiquitin-like modifiers (SUMOs) affects the behaviour of proteins involved in diverse cellular processes and is indispensable for cell survival. Proteomic studies…
(more)
▼ Post-translational modification by Small Ubiquitin-like modifiers (SUMOs) affects the behaviour of proteins involved in diverse cellular processes and is indispensable for cell survival. Proteomic studies have identified hundreds of putative targets of SUMO conjugation, but understanding the substrate-specific consequences of the modification requires knowledge of precise target lysines. Procedures for the high-throughput mapping of SUMO modification sites, however, are lacking. A method has been developed enabling global identification of sumoylated lysines by mass spectrometry (MS). The workflow entails in vivo conjugation to hexahistidine (6His)-tagged SUMO, in which the residue preceding the C-terminal Gly-Gly (diGly) has been mutated to lysine (6His-SUMOKGG). Lys-C digestion of 6His-SUMOKGG conjugates yields a diGly remnant on modified lysines that cannot be attributed to any other known modifier. This allows enrichment of SUMO remnant-modified peptides with a diGly-Lys (K-ε-GG)-specific antibody prior to their MS-based analysis. Application of the workflow enabled the unambiguous identification of 612 SUMO2 conjugation sites from the nuclear fraction of unperturbed human cells and 8262 sites from the same fraction of heat-stressed cells. Exposure to proteotoxic stress increased the extent of substrate sumoylation, and targeted proteins with similar functions or found within the same macromolecular complexes. These data provide a comprehensive resource for future research on the physiological role of sumoylation in health and disease.
Subjects/Keywords: SUMO; SUMO modification sites; Mass Spectrometry; Heat stress; Subcellular fractionation
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APA ·
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MLA ·
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Manager
APA (6th Edition):
Tammsalu, T. (2016). Global identification of SUMO modification sites. (Doctoral Dissertation). University of Dundee. Retrieved from https://discovery.dundee.ac.uk/en/studentTheses/5f8905a2-c31c-45ec-8173-4c91cf797342 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.805988
Chicago Manual of Style (16th Edition):
Tammsalu, Triin. “Global identification of SUMO modification sites.” 2016. Doctoral Dissertation, University of Dundee. Accessed January 18, 2021.
https://discovery.dundee.ac.uk/en/studentTheses/5f8905a2-c31c-45ec-8173-4c91cf797342 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.805988.
MLA Handbook (7th Edition):
Tammsalu, Triin. “Global identification of SUMO modification sites.” 2016. Web. 18 Jan 2021.
Vancouver:
Tammsalu T. Global identification of SUMO modification sites. [Internet] [Doctoral dissertation]. University of Dundee; 2016. [cited 2021 Jan 18].
Available from: https://discovery.dundee.ac.uk/en/studentTheses/5f8905a2-c31c-45ec-8173-4c91cf797342 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.805988.
Council of Science Editors:
Tammsalu T. Global identification of SUMO modification sites. [Doctoral Dissertation]. University of Dundee; 2016. Available from: https://discovery.dundee.ac.uk/en/studentTheses/5f8905a2-c31c-45ec-8173-4c91cf797342 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.805988
NSYSU
8.
Lee, Bi-yao.
Investigations of The Effects of Glucocorticoid Receptor SNPs and SUMO-2 Autoantibody in Patients with Systemic Lupus Erythematosus.
Degree: Master, Institute of Biomedical Sciences, 2008, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0730108-135015
► For more than fifty years glucocorticoids (GCs) has been used to treat a wide range of inflammatory diseases, such as allergies, asthma, rheumatoid arthritis, and…
(more)
▼ For more than fifty years glucocorticoids (GCs) has been used to treat a
wide range of inflammatory diseases, such as allergies, asthma, rheumatoid
arthritis, and autoimmune diseases, due to its potentiality on the antiinflammatory
and immunomodulatory effects. The anti-inflammation actions
of glucocorticoid were mediated by an intracellular receptor, glucocorticoid
receptor (GR), a member of the nuclear receptor family of ligand-dependent
transcription factor. Upon activation by their ligand, GRs translocated to the
nuclear and then bound to glucocorticoid responsive element (GRE) or
negative glucocorticoid responsive elemen (nGRE). The administration of
GCs depended on the acuity of disease and on the responses of patient
clinically. Although some Systemic Lupus Erythematosus (SLE) patients
given the maximal steroid doses, the response to the therapy remained
poorly, and thus called âglucocorticoid resistanceâ. Despite the fact that the
side effects and complications in SLE patients may result from the
restrictions of physic; it has been documented that there were some
relationships between the glucocorticoid resistance with the polymorphisms
of GR, and the levels of glucocorticoid receptor beta. However, no
significant differences in the GR polymorphisns (TthIII, ER22/23EK, N363S,
BclI and I559N) between controls and SLE patients were found and there
were no significant differences found on the levels of
SUMO-2 antibody
between patients with active and inactive SLE in this study. Neverthless, a
significant association on the the allelic polymorphism of BclI was observed
in patients with glucocorticoid resistance. Additionally, the expression of
GRβ in patients with SLE was higher than that of controls and the TthIII CT
genotype was associated with GRα expression.
Advisors/Committee Members: Deng-Chyang Wu (chair), Angela Chen (committee member), Hurng-Wern Huang (chair).
Subjects/Keywords: polymorphism; SUMO-2; glucocorticoid receptor; SLE
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APA (6th Edition):
Lee, B. (2008). Investigations of The Effects of Glucocorticoid Receptor SNPs and SUMO-2 Autoantibody in Patients with Systemic Lupus Erythematosus. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0730108-135015
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Lee, Bi-yao. “Investigations of The Effects of Glucocorticoid Receptor SNPs and SUMO-2 Autoantibody in Patients with Systemic Lupus Erythematosus.” 2008. Thesis, NSYSU. Accessed January 18, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0730108-135015.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Lee, Bi-yao. “Investigations of The Effects of Glucocorticoid Receptor SNPs and SUMO-2 Autoantibody in Patients with Systemic Lupus Erythematosus.” 2008. Web. 18 Jan 2021.
Vancouver:
Lee B. Investigations of The Effects of Glucocorticoid Receptor SNPs and SUMO-2 Autoantibody in Patients with Systemic Lupus Erythematosus. [Internet] [Thesis]. NSYSU; 2008. [cited 2021 Jan 18].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0730108-135015.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Lee B. Investigations of The Effects of Glucocorticoid Receptor SNPs and SUMO-2 Autoantibody in Patients with Systemic Lupus Erythematosus. [Thesis]. NSYSU; 2008. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0730108-135015
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
9.
Hsu, Chih-wei.
Investigation of the SUMOylation sites of Gfi1.
Degree: Master, Institute of Biomedical Sciences, 2015, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0421115-100738
► The transcriptional repressor Growth Factor Independence 1 (Gfi1) regulates many biological functions, such as development of granulocytes, T-cell differentiation, macrophage-dependent cytokine production and the development…
(more)
▼ The transcriptional repressor Growth Factor Independence 1 (Gfi1) regulates many biological functions, such as development of granulocytes, T-cell differentiation, macrophage-dependent cytokine production and the development of inner ear hair cells. It contains six C-terminal C2H2-type zinc-finger domains and a characteristic stretch of 20 amino acids, called the SNAG-domain, at its N-terminus. The presence of C2H2 zinc fingers suggested a function for Gfi1 as a DNA binding transcription factor. Zinc fingers 3â5 of Gfi1 are necessary for binding to its cognate consensus DNA recognition sequence taAATCac(t/a)gca, and zinc fingers 1, 2 and 6 have a role in interaction with other proteins. The small ubiquitin-related modifier (
SUMO) has many roles in cellular biology including regulation of transcription factors. We found that there are four major SUMOylation predicted sites (ΨKXE) in Gfi-1 protein analyzed with bioinformatics. The SUMOylation site of the Gfi-1 protein was therefore investigated in the present studies. To identify the SUMOylation sites of Gfi1, mutants were used in in vitro SUMOylation assay. Fluorescence microscopy assay was used to investigate the interaction between Gfi1 and
SUMO. Results suggest that Gfi1 and
SUMO are colocalized partially and Gfi1 is a target protein for
SUMO modification.
Advisors/Committee Members: Ping-I Hsu (chair), Angela Chen (committee member), Hung-Wen Huamg (chair).
Subjects/Keywords: SIM; Colocalization; In vitro SUMOylation; SUMO; Gfi1
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APA (6th Edition):
Hsu, C. (2015). Investigation of the SUMOylation sites of Gfi1. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0421115-100738
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Hsu, Chih-wei. “Investigation of the SUMOylation sites of Gfi1.” 2015. Thesis, NSYSU. Accessed January 18, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0421115-100738.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Hsu, Chih-wei. “Investigation of the SUMOylation sites of Gfi1.” 2015. Web. 18 Jan 2021.
Vancouver:
Hsu C. Investigation of the SUMOylation sites of Gfi1. [Internet] [Thesis]. NSYSU; 2015. [cited 2021 Jan 18].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0421115-100738.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Hsu C. Investigation of the SUMOylation sites of Gfi1. [Thesis]. NSYSU; 2015. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0421115-100738
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
10.
Wu, Chun-Yi.
Interaction between KLIP1 and SUMO-1.
Degree: Master, Biological Sciences, 2011, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0905111-105014
► Nuclear protein KLIP1 cooperates with myeloid leukemia factor 1 (MLF1) to inhibit the programmed cell death resulting in tumor formation. It also inhibits the activity…
(more)
▼ Nuclear protein KLIP1 cooperates with myeloid leukemia factor 1 (MLF1) to inhibit the programmed cell death resulting in tumor formation. It also inhibits the activity of thymidine kinase promoter of Kaposiâs sarcoma-associated Herpes Virus. KLIP1 functions as a centromere protein, hence acquires its name as CENP-U or CENP-50, to regulate the separation of sister-chromatids during mitosis. These results indicate that KLIP1 plays important roles in regulation of transcription and cell cycle. In this study, six potential
SUMO modification sites, K33, K63, K126, K127, K185 and K210, were identified bioinformatically using SUMOplot. Many reports address that
SUMO modification alters the transcriptional activity, protein-protein interaction, the subcellular localization and stability of its target protein. Recent data suggest that
SUMO is required for centromere binding protein to mediate proper mitotic spindle attachment to the kinetochore, and previous research suggest that there has a
SUMO-interaction motif (SIM) in KLIP1 protein sequence. To reveal the interaction between KLIP1 and
SUMO-1, and study its effects on KLIP1 function, we co-express GFP-KLIP1 and His-tagged
SUMO-1 in HEK 293 cells. After affinity purification of SUMOylated proteins from transfected cells using nickel conjugated beads and subsequent western blotted with anti-GFP. The results indicated the interaction between KLIP1 and
SUMO-1 in co-transfected cells. Our confocal microscopy imaging also found colocalization of GFP-KLIP1 with RFP-
SUMO-1 nuclear foci. In addition, we failed to detect the interaction between
SUMO-1 and mutant KLIP1-M6 ,whose six potential
SUMO modified lysine residues were mutated to arginine. Furthermore, we found a distinct nuclear localization of GFP-KLIP1-M6 as compared to the image of wildtype GFP-KLIP1, which show a significant higher frequency of colocalization with RFP-
SUMO-1 foci. Taken together, our data suggest the interaction between KLIP1 and
SUMO-1 may be related to these six potential lysine residues, which upon mutation blocks its colocalization with
SUMO-1 in nuclear foci. The biological significance of their interaction are awaits for further investigation.
Advisors/Committee Members: YI-REN HONG (chair), Jiin-Tsuey Cheng (committee member), Angela Chen (chair).
Subjects/Keywords: KLIP1; SUMO-1; site-directed mutagenesis
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to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Wu, C. (2011). Interaction between KLIP1 and SUMO-1. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0905111-105014
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Wu, Chun-Yi. “Interaction between KLIP1 and SUMO-1.” 2011. Thesis, NSYSU. Accessed January 18, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0905111-105014.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Wu, Chun-Yi. “Interaction between KLIP1 and SUMO-1.” 2011. Web. 18 Jan 2021.
Vancouver:
Wu C. Interaction between KLIP1 and SUMO-1. [Internet] [Thesis]. NSYSU; 2011. [cited 2021 Jan 18].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0905111-105014.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Wu C. Interaction between KLIP1 and SUMO-1. [Thesis]. NSYSU; 2011. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0905111-105014
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Rochester Institute of Technology
11.
Prokop, Matthew.
Routing protocol evaluation and development of a fully functional simulation environment for vehicular ad hoc networks.
Degree: Computer Engineering, 2011, Rochester Institute of Technology
URL: https://scholarworks.rit.edu/theses/3182
► A Vehicular Ad-hoc Networks (VANET) is an area of wireless technologies that are attracting a great deal of interest. There are still several areas of…
(more)
▼ A Vehicular Ad-hoc Networks (VANET) is an area of wireless technologies that are attracting a great deal of interest. There are still several areas of VANETS, such as medium access control, security and routing protocols, that lack large amounts of research. There is also a lack of freely available simulators that can quickly and accurately simulate VANETs. One of the two main goals of this
thesis was to develop a freely available VANET simulator and to evaluate popular mobile ad-hoc network routing protocols in several VANET scenarios. The VANET simulator consisted of a network simulator, a traffic (mobility simulator) and used a client-server application to keep the two simulators in sync. The VANET
simulator also models buildings in order to create a more realistic wireless network environment. The second main goal of this thesis was to provide an evaluation of the routing protocols that are commonly used in mobile ad-hoc networks, which will apply to VANETs. Ad-Hoc Distance Vector routing (AODV), Dynamic Source Routing (DSR) and Dynamic MANET On-demand (DYMO) were initially simulated in a city, country, and highway environment in order to provide an overall evaluation.
Advisors/Committee Members: Kwasinski, Andres.
Subjects/Keywords: AODV; DSR; DYMO; Omnet++; SUMO; VANET
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APA (6th Edition):
Prokop, M. (2011). Routing protocol evaluation and development of a fully functional simulation environment for vehicular ad hoc networks. (Thesis). Rochester Institute of Technology. Retrieved from https://scholarworks.rit.edu/theses/3182
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Prokop, Matthew. “Routing protocol evaluation and development of a fully functional simulation environment for vehicular ad hoc networks.” 2011. Thesis, Rochester Institute of Technology. Accessed January 18, 2021.
https://scholarworks.rit.edu/theses/3182.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Prokop, Matthew. “Routing protocol evaluation and development of a fully functional simulation environment for vehicular ad hoc networks.” 2011. Web. 18 Jan 2021.
Vancouver:
Prokop M. Routing protocol evaluation and development of a fully functional simulation environment for vehicular ad hoc networks. [Internet] [Thesis]. Rochester Institute of Technology; 2011. [cited 2021 Jan 18].
Available from: https://scholarworks.rit.edu/theses/3182.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Prokop M. Routing protocol evaluation and development of a fully functional simulation environment for vehicular ad hoc networks. [Thesis]. Rochester Institute of Technology; 2011. Available from: https://scholarworks.rit.edu/theses/3182
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universiteit Utrecht
12.
Poot, B.W.A.
Norm-based distributed controllers in a Multi-Agent System.
Degree: 2015, Universiteit Utrecht
URL: http://dspace.library.uu.nl:8080/handle/1874/320413
► Autonomous vehicles will most likely participate in traffic in the near future. These autonomous vehicles have private goals they want to achieve, which might conflict…
(more)
▼ Autonomous vehicles will most likely participate in traffic in the near future. These autonomous vehicles have private goals they want to achieve, which might conflict with the goals of the traffic system designers. To ensure that the goals of the system are met, the vehicle behavior needs to be influenced. Since the vehicles are autonomous, their behavior cannot be controlled di- rectly. One way of influencing behavior indirectly is through norms. A norm is a violable rule that describes correct behavior. Vehicles who do not com- ply with the norm may receive a sanction, such as a monetary fine. The norms are issued to the vehicles by traffic control systems, which monitor the vehicles and sanction them when norm violations are detected. In this thesis, we present a formal model and a corresponding imple- mentation for a norm-based multi-agent system for traffic. We created a formal model of vehicles, traffic regulations and traffic controllers and im- plemented this in an extension of a state-of-the-art traffic simulator named
SUMO. The features of this extension are presented in several demonstrative experiments.
Advisors/Committee Members: Dastani, M.M., Meyer, J-J.Ch..
Subjects/Keywords: Multi-Agent Systems; Norms; Traffic; SUMO
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APA (6th Edition):
Poot, B. W. A. (2015). Norm-based distributed controllers in a Multi-Agent System. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/320413
Chicago Manual of Style (16th Edition):
Poot, B W A. “Norm-based distributed controllers in a Multi-Agent System.” 2015. Masters Thesis, Universiteit Utrecht. Accessed January 18, 2021.
http://dspace.library.uu.nl:8080/handle/1874/320413.
MLA Handbook (7th Edition):
Poot, B W A. “Norm-based distributed controllers in a Multi-Agent System.” 2015. Web. 18 Jan 2021.
Vancouver:
Poot BWA. Norm-based distributed controllers in a Multi-Agent System. [Internet] [Masters thesis]. Universiteit Utrecht; 2015. [cited 2021 Jan 18].
Available from: http://dspace.library.uu.nl:8080/handle/1874/320413.
Council of Science Editors:
Poot BWA. Norm-based distributed controllers in a Multi-Agent System. [Masters Thesis]. Universiteit Utrecht; 2015. Available from: http://dspace.library.uu.nl:8080/handle/1874/320413
Universidade Nova
13.
Bértolo, Diana Fernandes Quitério.
Aplicação de quitosano na redução do escurecimento enzimático em sumo de maçã não pasteurizado (estabilizado por hiperpressão).
Degree: 2011, Universidade Nova
URL: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/6718
► Dissertação para obtenção do Grau de Mestre em Tecnologia e Segurança Alimentar
A maçã, sendo um dos frutos com maior produção em Portugal, detentor de…
(more)
▼ Dissertação para obtenção do Grau de Mestre em
Tecnologia e Segurança Alimentar
A maçã, sendo um dos frutos com maior produção em Portugal, detentor de elevado valor nutricional, apresenta um potencial comercial e industrial elevado. O consumo de sumo de maçã natural, não pasteurizado e estabilizado por hiperpressão, está em franca expansão, sendo muito apreciado pelo consumidor. No entanto, o seu tempo de prateleira é consideravelmente curto, apresentando um escurecimento enzimático acelerado. Aspecto que é considerado indesejável pelo consumidor.
Equacionando as propriedades antioxidantes e antimicrobianas do quitosano, um polissacárido proveniente da quitina, neste trabalho caracterizou-se a acção de duas formas de adição desta entidade química (em solução com ácido ascórbico e em pó) e três concentrações (0,6 g/L, 0,7g/L e 0,8g/L) no sumo de maçã. Verificou-se uma redução do escurecimento enzimático do sumo após a adição das formas e concentrações de quitosano, mantendo o mesmo, uma cor mais clara, quando comparado com o sumo de maçã que já existe no mercado. Grande parte das características do sumo foram mantidas com a adição do quitosano, não existindo diferenças entre concentrações ou entre formas de adição. De acordo com resultados obtidos por um painel de provadores não treinados, a aceitação em parâmetros como a cor, sabor e doçura persistiu. Ainda assim, verificaram-se ligeiras variações em parâmetros como o pH, acidez titulável e no teor de ácido ascórbico. Apesar de levar a uma redução do escurecimento enzimático, não ocorreu um aumento do período de conservação do sumo de maçã, pois mesmo com adição do quitosano as contagens microbiológicas persistiram elevadas para as diferentes concentrações e formas de quitosano adicionadas.
Advisors/Committee Members: Lidon, Fernando, Lara, Claudia.
Subjects/Keywords: Conservação; Escurecimento enzimático; Maçã; Sumo; Quitosano
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APA (6th Edition):
Bértolo, D. F. Q. (2011). Aplicação de quitosano na redução do escurecimento enzimático em sumo de maçã não pasteurizado (estabilizado por hiperpressão). (Thesis). Universidade Nova. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/6718
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Bértolo, Diana Fernandes Quitério. “Aplicação de quitosano na redução do escurecimento enzimático em sumo de maçã não pasteurizado (estabilizado por hiperpressão).” 2011. Thesis, Universidade Nova. Accessed January 18, 2021.
http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/6718.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Bértolo, Diana Fernandes Quitério. “Aplicação de quitosano na redução do escurecimento enzimático em sumo de maçã não pasteurizado (estabilizado por hiperpressão).” 2011. Web. 18 Jan 2021.
Vancouver:
Bértolo DFQ. Aplicação de quitosano na redução do escurecimento enzimático em sumo de maçã não pasteurizado (estabilizado por hiperpressão). [Internet] [Thesis]. Universidade Nova; 2011. [cited 2021 Jan 18].
Available from: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/6718.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Bértolo DFQ. Aplicação de quitosano na redução do escurecimento enzimático em sumo de maçã não pasteurizado (estabilizado por hiperpressão). [Thesis]. Universidade Nova; 2011. Available from: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/6718
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Dundee
14.
Chua, Shijia Joy.
Establishing the role of RNF4 in the vertebrate DNA damage response.
Degree: PhD, 2012, University of Dundee
URL: https://discovery.dundee.ac.uk/en/studentTheses/20679582-0300-40ac-8d30-e733210c76b4
;
https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.578870
► RNF4 belongs to the family of SUMO-targeted ubiquitin E3 ligases (STUbLs). The role of STUbLs in maintaining genomic stability was first discovered in yeast. Theyeast…
(more)
▼ RNF4 belongs to the family of SUMO-targeted ubiquitin E3 ligases (STUbLs). The role of STUbLs in maintaining genomic stability was first discovered in yeast. Theyeast STUbL mutants displayed genomic instability, elevated mutation rates, sensitivity to DNA damaging agents and also demonstrated synthetic lethality with other DNA repair genes. Although the role of vertebrate RNF4 in the DNA damage response was not yet established, it could rescue the Schizosaccaromyces pombe STUbL mutant phenotypes, showing that RNF4 is a functional homologue of the yeast STUbL proteins,and that it might be implicated in the vertebrate DNA damage response.A homozygous knockout of RNF4 in the DT40 chicken lymphocyte cell line was generated to investigate the involvement of vertebrate RNF4 in protecting cells against DNA damage. Although the complete loss of RNF4 did not affect cell proliferation or cell cycle distribution, the RNF4 -/- cells exhibited a selective hypersensitivity to some S-phase specific DNA damaging agents. This hypersensitivity could be rescued by introducing an ortholog of RNF4 from another vertebrate species, and this was dependent on a functional ubiquitin E3 ligase activity of RNF4.To explore the physiological function of RNF4 in the context of a wholeorganism, Danio rerio was chosen as an in vivo model. Danio rerio RNF4 sharedsimilar in vitro biochemical characteristics as RNF4 from other vertebrates – it was able to autoubiquitylate itself and also ubiquitylate SUMO2 chains. In Danio rerio, RNF4 is a maternally provided gene and is highly expressed in the adult gonads. In the ovaries, RNF4 expression was restricted to the early stage oocytes, suggesting a possible role in oocyte development. Loss-of-function studies in Danio rerio were performed using morpholino knockdown and zinc-finger knockout technologies, and the depletion of RNF4 in zebrafish did not affect early embryonic development or viability of the animal.The results presented in this thesis suggests that while vertebrate RNF4 is notlikely to be an essential gene in some vertebrates, it plays a role in the DNA damage response and might be implicated in gonad development in Danio rerio. The zinc-finger knockout model has just been established and a more in-depth analysis is necessary to shed more light on the in vivo functions of RNF4.
Subjects/Keywords: 572.8; DNA damage; Ubiquitin; SUMO; Zebrafish; DT40
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APA (6th Edition):
Chua, S. J. (2012). Establishing the role of RNF4 in the vertebrate DNA damage response. (Doctoral Dissertation). University of Dundee. Retrieved from https://discovery.dundee.ac.uk/en/studentTheses/20679582-0300-40ac-8d30-e733210c76b4 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.578870
Chicago Manual of Style (16th Edition):
Chua, Shijia Joy. “Establishing the role of RNF4 in the vertebrate DNA damage response.” 2012. Doctoral Dissertation, University of Dundee. Accessed January 18, 2021.
https://discovery.dundee.ac.uk/en/studentTheses/20679582-0300-40ac-8d30-e733210c76b4 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.578870.
MLA Handbook (7th Edition):
Chua, Shijia Joy. “Establishing the role of RNF4 in the vertebrate DNA damage response.” 2012. Web. 18 Jan 2021.
Vancouver:
Chua SJ. Establishing the role of RNF4 in the vertebrate DNA damage response. [Internet] [Doctoral dissertation]. University of Dundee; 2012. [cited 2021 Jan 18].
Available from: https://discovery.dundee.ac.uk/en/studentTheses/20679582-0300-40ac-8d30-e733210c76b4 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.578870.
Council of Science Editors:
Chua SJ. Establishing the role of RNF4 in the vertebrate DNA damage response. [Doctoral Dissertation]. University of Dundee; 2012. Available from: https://discovery.dundee.ac.uk/en/studentTheses/20679582-0300-40ac-8d30-e733210c76b4 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.578870
15.
Dangoumau, Audrey.
Etude de la voie de la SUMOylation dans la sclérose latérale amyotrophique associée à des mutations de SOD1 : Study of pathway of SUMOylation in Amyotrophic Lateral Sclerosis associated with SOD1 gene mutation.
Degree: Docteur es, Sciences de la Vie et de la Santé, 2014, Université François-Rabelais de Tours
URL: http://www.theses.fr/2014TOUR3308
► La sclérose Latérale Amyotrophique (SLA) est une maladie neurodégénérative des motoneurones impliquant des facteurs environnementaux et génétiques. Notre étude porte sur l’étude des relations entre…
(more)
▼ La sclérose Latérale Amyotrophique (SLA) est une maladie neurodégénérative des motoneurones impliquant des facteurs environnementaux et génétiques. Notre étude porte sur l’étude des relations entre la voie de la SUMOylation post-Traductionnelle des protéines et les effets du stress oxydant et de mutants SOD1. Nous montrons tout d’abord que 2 nouveaux mutants, SOD1V31A et SOD1E121G identifiés chez des patients SLA à évolution lente, entraîne la formation d’agrégats cellulaires Ub/SUMO dans la formation des agrégats était suggérée. Nous montrons 1) que les NSC-34 exposées à un stress oxydant et exprimant SOD1 mutée présentent une modification d’expression de plusieurs gènes des voies de l’Ub/SUMO ; 2) que l’expression de SOD1 mutée réduit le pool de protéine SUMO-1 libre dans les cellules motoneuronales, possible conséquence d’une séquestration dans les agrégats ; 3) qu’inhiber la SUMOylation de SOD1 mutée réduit la quantité de cellules avec agrégats. Nos résultats indiquent qu’une meilleure connaissance de la voie de SUMO pourrait conduire à de nouvelles cibles thérapeutiques intéressantes dans la SLA.
Amyotrophic Lateral Sclerosis (ALS) is a neurodegenerative disease of motor neurones involving a combination of environmental and genetics factors. Ours work focuses on the relathionship between the SUMOylation pathway and the effects of oxidative stress and SOD1 mutants. We first show that 2 new mutants, SOD1V31A and SOD1E121G identified in ALS patients with a slowly progressive disease, induce the formation of Ub/SUMO positive aggregates in motor neuronal cells NSC-34. The implication of the Ub/SUMO pathways has been proposed in the formation of aggregates in ALS. We show 1) modification of expression of several genes of the Ub/SUMO pathways in NSC-34 exposed to oxidative stress and expressing various mutated SOD1 proteins; 2) that the expression of mutants SOD1 reduces free-SUMO1 concentration in motor neuronal cell, perhaps by a sequestration in aggregates; 3) that the inhibition of SUMIylation of various mutants SOD1 reduces the amount of cells with aggregates. Our results support further studies on the SUMO pathway that may lead to new therapeutics targets in ALS.
Advisors/Committee Members: Vourc'h, Patrick (thesis director).
Subjects/Keywords: SLA; Motoneurones; SOD1; Agrégats; SUMO; Ubiquitine; ALS; Motor neuron; SOD1; Aggregates; SUMO; Ubiquitin
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APA (6th Edition):
Dangoumau, A. (2014). Etude de la voie de la SUMOylation dans la sclérose latérale amyotrophique associée à des mutations de SOD1 : Study of pathway of SUMOylation in Amyotrophic Lateral Sclerosis associated with SOD1 gene mutation. (Doctoral Dissertation). Université François-Rabelais de Tours. Retrieved from http://www.theses.fr/2014TOUR3308
Chicago Manual of Style (16th Edition):
Dangoumau, Audrey. “Etude de la voie de la SUMOylation dans la sclérose latérale amyotrophique associée à des mutations de SOD1 : Study of pathway of SUMOylation in Amyotrophic Lateral Sclerosis associated with SOD1 gene mutation.” 2014. Doctoral Dissertation, Université François-Rabelais de Tours. Accessed January 18, 2021.
http://www.theses.fr/2014TOUR3308.
MLA Handbook (7th Edition):
Dangoumau, Audrey. “Etude de la voie de la SUMOylation dans la sclérose latérale amyotrophique associée à des mutations de SOD1 : Study of pathway of SUMOylation in Amyotrophic Lateral Sclerosis associated with SOD1 gene mutation.” 2014. Web. 18 Jan 2021.
Vancouver:
Dangoumau A. Etude de la voie de la SUMOylation dans la sclérose latérale amyotrophique associée à des mutations de SOD1 : Study of pathway of SUMOylation in Amyotrophic Lateral Sclerosis associated with SOD1 gene mutation. [Internet] [Doctoral dissertation]. Université François-Rabelais de Tours; 2014. [cited 2021 Jan 18].
Available from: http://www.theses.fr/2014TOUR3308.
Council of Science Editors:
Dangoumau A. Etude de la voie de la SUMOylation dans la sclérose latérale amyotrophique associée à des mutations de SOD1 : Study of pathway of SUMOylation in Amyotrophic Lateral Sclerosis associated with SOD1 gene mutation. [Doctoral Dissertation]. Université François-Rabelais de Tours; 2014. Available from: http://www.theses.fr/2014TOUR3308
Université Montpellier II
16.
Nait Achour, Thiziri.
Les rôles de la SUMO protéase SENP2 et du corépresseur LCoR dans la signalisation œstrogénique : Role of the SENP2 SUMO protease and LCoR in estrogen signalling.
Degree: Docteur es, Biologie Santé, 2011, Université Montpellier II
URL: http://www.theses.fr/2011MON20206
► Les œstrogènes sont impliqués dans la prolifération des cellules épithéliales du sein normal et l'exposition prolongée à ces hormones s'accompagne d'une augmentation du risque de…
(more)
▼ Les œstrogènes sont impliqués dans la prolifération des cellules épithéliales du sein normal et l'exposition prolongée à ces hormones s'accompagne d'une augmentation du risque de développement de cancer du sein. Les œstrogènes exercent leurs effets via les récepteurs des œstrogènes (REs). L'activité de ces récepteurs est finement régulée par un grand nombre de cofacteurs transcriptionnels, mais également par les modifications post-traductionnelles. Mon travail de thèse a eu pour objectif la compréhension de l'impact de ces deux niveaux de régulation sur la signalisation œstrogénique. Il a été récemment décrit que la sumoylation affectait de manière drastique l'activité du RE. La sumoylation est une modification dont le caractère réversible est assuré par des isopeptidases appelé SENPs (SENtrin Proteases). Dans une première étude nous avons montré que SENP2 pouvait fortement réprimer l'activité transcriptionnelle dépendante des œstrogènes ainsi que la prolifération cellulaire. Dans une seconde étude, nous nous sommes attelés à mieux caractériser les mécanismes d'action à l'origine du caractère répresseur du cofacteur transcriptionnel LCoR (Ligand-dependent Corepressor). Nous nous sommes plus précisément intéressés aux relations existant entre LCoR et un autre cofacteur du RE, RIP140 (Receptor Interacting Protein of 140 kDa) répresseur majeur de l'activité œstrogénique. Nous avons pu caractériser, outre les modes de recrutement des deux protéines, les modulations d'expression exercées par les deux cofacteurs. L'ensemble de nos travaux identifie de nouveaux cofacteurs des REs et contribue à une meilleure compréhension de la signalisation œstrogénique.
Estrogens are involved in the proliferation of normal breast epithelial cells. The prolonged exposure to these hormones comes along with an increase of the risk of breast cancer.development. Estrogen receptors (ERs) mediate the effects of estrogens. The activity of these receptors is finely tuned by a large number of transcriptional cofactors, but also by post-translational modifications. This work aimed at understanding the impact of these regulations on estrogenic signalling. It was recently described that sumoylation could strongly affect ER-dependent activity. SUMO conjugation is a dynamic process which is reversed by SUMO specific proteases also known as SENtrin Proteases (SENPs). In a first study, we investigated the role of SENP2, in ER-dependent transcriptional activity. We showed that SENP2 could acts as a transcriptional cofactor independently of its catalytic activity by strongly repressing ER-dependent transcriptional activity. We also provided evidence for a role in in breast cancer cell line proliferation. In a second part of the work we investigated the mechanism of action of the transcriptional cofactor LCoR (Ligand-dependent Corepressor) with a specific emphasis on the relationship between LCoR and another ER cofactor, RIP140 (Receptor Interacting Protein of 140 kDa). We characterized a crossed expression modulation of the two transcription cofactors.…
Advisors/Committee Members: Jalaguier, Stephan (thesis director).
Subjects/Keywords: Senp; Er; Oestrogènes; Sumo; LCoR; Rip140; Senp; Er; Estrogens; Sumo; LCoR; Rip140
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APA ·
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APA (6th Edition):
Nait Achour, T. (2011). Les rôles de la SUMO protéase SENP2 et du corépresseur LCoR dans la signalisation œstrogénique : Role of the SENP2 SUMO protease and LCoR in estrogen signalling. (Doctoral Dissertation). Université Montpellier II. Retrieved from http://www.theses.fr/2011MON20206
Chicago Manual of Style (16th Edition):
Nait Achour, Thiziri. “Les rôles de la SUMO protéase SENP2 et du corépresseur LCoR dans la signalisation œstrogénique : Role of the SENP2 SUMO protease and LCoR in estrogen signalling.” 2011. Doctoral Dissertation, Université Montpellier II. Accessed January 18, 2021.
http://www.theses.fr/2011MON20206.
MLA Handbook (7th Edition):
Nait Achour, Thiziri. “Les rôles de la SUMO protéase SENP2 et du corépresseur LCoR dans la signalisation œstrogénique : Role of the SENP2 SUMO protease and LCoR in estrogen signalling.” 2011. Web. 18 Jan 2021.
Vancouver:
Nait Achour T. Les rôles de la SUMO protéase SENP2 et du corépresseur LCoR dans la signalisation œstrogénique : Role of the SENP2 SUMO protease and LCoR in estrogen signalling. [Internet] [Doctoral dissertation]. Université Montpellier II; 2011. [cited 2021 Jan 18].
Available from: http://www.theses.fr/2011MON20206.
Council of Science Editors:
Nait Achour T. Les rôles de la SUMO protéase SENP2 et du corépresseur LCoR dans la signalisation œstrogénique : Role of the SENP2 SUMO protease and LCoR in estrogen signalling. [Doctoral Dissertation]. Université Montpellier II; 2011. Available from: http://www.theses.fr/2011MON20206
University of Vienna
17.
Hubner, Michaela.
A novel approach to identify substrates for the sumoylated Ubc9.
Degree: 2010, University of Vienna
URL: http://othes.univie.ac.at/10370/
► Unser Labor konnte zeigen, dass Sumoylierung des E2 konjugierenden Enzyms Ubc9 zur Diskriminierung von SUMO Substraten in Säugetieren beiträgt. Sumoyliertes Ubc9 (S*Ubc9) zeigt keinen Effekt…
(more)
▼ Unser Labor konnte zeigen, dass Sumoylierung des E2 konjugierenden Enzyms Ubc9 zur Diskriminierung von SUMO Substraten in Säugetieren beiträgt. Sumoyliertes Ubc9 (S*Ubc9) zeigt keinen Effekt in der Modifikation der meisten SUMO Substrate, aber interessanterweise inhibiert es die Sumoylierung von RanGAP1 und verstärkt signifikant die Sumoylierung des Transkriptionsregulators Sp100 (Knipscheer et al., 2008). Sp100 war allerdings das einzige Substrat zu dieser Zeit, das mit dem sumoylierten Ubc9 verstärkt modifiziert wurde. In dieser Arbeit wurden Protein Microarrays (“ProtoArray® Human Protein Microarray v5.0” von Invitrogen) als neuer Ansatz zur Identifizierung weiterer Substrate für das S*Ubc9 verwendet. Neu identifizierte Substrate wurden in unserem in vitro System verifiziert und werden Einblicke in die biologische Funktion des S*Ubc9 liefern.
Our laboratory has discovered a novel mechanism of how sumoylation of the E2 conjugating enzyme Ubc9 contributes to target discrimination in mammals. Whereas sumoylated Ubc9 (S*Ubc9) does not interfere with the sumoylation of most SUMO substrates, it impairs modification of RanGAP1 and significantly enhances modification of the transcriptional regulator Sp100 (Knipscheer et al., 2008). However, Sp100 was the only substrate so far preferentially targeted by S*Ubc9. In this work, protein microarray chips (“ProtoArray® Human Protein Microarray v5.0”) purchasable from Invitrogen were applied for sumoylation reactions as a novel approach to identify substrates for the S*Ubc9. Newly identified substrates were verified in our in vitro system and will provide novel insights in the biological function of S*Ubc9.
Subjects/Keywords: 42.13 Molekularbiologie; SUMO / E2 konjugierendes Enzym Ubc9; SUMO / E2 conjugating enzyme Ubc9
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APA ·
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CSE |
Export
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APA (6th Edition):
Hubner, M. (2010). A novel approach to identify substrates for the sumoylated Ubc9. (Thesis). University of Vienna. Retrieved from http://othes.univie.ac.at/10370/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Hubner, Michaela. “A novel approach to identify substrates for the sumoylated Ubc9.” 2010. Thesis, University of Vienna. Accessed January 18, 2021.
http://othes.univie.ac.at/10370/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Hubner, Michaela. “A novel approach to identify substrates for the sumoylated Ubc9.” 2010. Web. 18 Jan 2021.
Vancouver:
Hubner M. A novel approach to identify substrates for the sumoylated Ubc9. [Internet] [Thesis]. University of Vienna; 2010. [cited 2021 Jan 18].
Available from: http://othes.univie.ac.at/10370/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Hubner M. A novel approach to identify substrates for the sumoylated Ubc9. [Thesis]. University of Vienna; 2010. Available from: http://othes.univie.ac.at/10370/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
18.
Auchter, Morgan.
Implication de la topoisomérase IIIa dans la stabilité chromosomique au cours de la recombinaison télomérique des cellules cancéreuses : Translating French determiners in Arabic : contrastive study.
Degree: Docteur es, Génétique, 2013, Aix Marseille Université
URL: http://www.theses.fr/2013AIXM4008
► Dans les cellules somatiques, les télomères s'érodent à chaque division cellulaire. Ce processus appelé « Sénescence Réplicative» est contrebalancé de manière basale chez la levure…
(more)
▼ Dans les cellules somatiques, les télomères s'érodent à chaque division cellulaire. Ce processus appelé « Sénescence Réplicative» est contrebalancé de manière basale chez la levure bourgeonnante S. cerevisiae par l'action de la télomérase qui, alors qu'elle est inactive dans les cellules somatiques des eucaryotes supérieures, est activée dans 85% des cancers. Un autre mécanisme impliqué dans les 15% des cas de cancer restants et est appelé Alternative Lengthening of Telomere (ALT). Dans ce processus, le maintien des télomères est assuré par des mécanismes de recombinaison télomérique induisant des échanges de séquences télomériques de chromatides sœurs (T-SCE).Nous avons évalué l'existence d'ALT dans la LLC-B connue pour rarement exprimer la télomérase. Nous avons montrer que 90% des patients LLC-B présentent une diminution de l'expression de TopoIIIα corrélée à une méthylation plus importante des îlots CpG de la région promotrice du gène suggérant que dans les LLC-B le maintien des télomères est défectueux.Nous avons étudié l'implication de la SUMOylation de TopoIIIα/Top3 dans les mécanismes de régulation du ALT. Nous avons montré que TopoIIIα était SUMOylée in vitro et in vivo au sein des cellules U2-OS ALT. Nous avons aussi observé chez S. cerevisiae que Top3 ne serait SUMOylée qu'en absence d'une activité télomérase. Nos résultats suggèrent que la SUMOylation de TopoIIIα augmenterait son activité in vitro et in vivo en diminuant son affinité pour les télomères une fois la recombinaison achevée et qu'elle serait requise pour son accumulation dans les APBs mais pas pour leur formation.
In somatic cells, telomeres erode with each cell division. This process named « Replicative Senescence » is basically counterbalanced in the budding yeast Saccharomyces cerevisiae by the action of telomerase which, while it is inactive in somatic cells of higher eukaryotes is activated in 85 % of cancer cases. Another process of telomere maintenance is involved in 15% of remaining cancer cases and is called Alternative Lengthening of Telomere (ALT). In this process, telomere maintenance is provided by telomeric recombination mechanisms inducing exchange of telomeric sister chromatid (T-SCE).We assessed the existence of an ALT mechanism in B-CLL known to rarely express telomerase. We have shown that 90% of B-CLL patients have a decreased expression of TopoIIIα correlated with largest methylation of CpG islands of the gene promoter region. Our results suggest that in B-CLL, telomere maintenance is defective either by telomerase or ALT mechanism.We investigated the involvement of post- SUMOylation of TopoIIIα/Top3 in mechanisms regulating ALT phenomenon. We have shown that TopoIIIα was SUMOylated in vitro and in vivo in U2-OS ALT cells. We also observed in S. cerevisiae that Top3p might be SUMOylated in absence of telomerase activity. Our results suggested that the SUMOylation of TopoIIIα increased its activity in vitro and in vivo by reducing its affinity for telomeres once recombination occurred and would be required for its…
Advisors/Committee Members: Geli, Vincent (thesis director).
Subjects/Keywords: Télomères; Topoisomérase; Recombinaison; Sumo; ALT; LLC-B; Telomeres; Topoisomerase; Recombination; Sumo; ALT; CLL-B; 570
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APA ·
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APA (6th Edition):
Auchter, M. (2013). Implication de la topoisomérase IIIa dans la stabilité chromosomique au cours de la recombinaison télomérique des cellules cancéreuses : Translating French determiners in Arabic : contrastive study. (Doctoral Dissertation). Aix Marseille Université. Retrieved from http://www.theses.fr/2013AIXM4008
Chicago Manual of Style (16th Edition):
Auchter, Morgan. “Implication de la topoisomérase IIIa dans la stabilité chromosomique au cours de la recombinaison télomérique des cellules cancéreuses : Translating French determiners in Arabic : contrastive study.” 2013. Doctoral Dissertation, Aix Marseille Université. Accessed January 18, 2021.
http://www.theses.fr/2013AIXM4008.
MLA Handbook (7th Edition):
Auchter, Morgan. “Implication de la topoisomérase IIIa dans la stabilité chromosomique au cours de la recombinaison télomérique des cellules cancéreuses : Translating French determiners in Arabic : contrastive study.” 2013. Web. 18 Jan 2021.
Vancouver:
Auchter M. Implication de la topoisomérase IIIa dans la stabilité chromosomique au cours de la recombinaison télomérique des cellules cancéreuses : Translating French determiners in Arabic : contrastive study. [Internet] [Doctoral dissertation]. Aix Marseille Université 2013. [cited 2021 Jan 18].
Available from: http://www.theses.fr/2013AIXM4008.
Council of Science Editors:
Auchter M. Implication de la topoisomérase IIIa dans la stabilité chromosomique au cours de la recombinaison télomérique des cellules cancéreuses : Translating French determiners in Arabic : contrastive study. [Doctoral Dissertation]. Aix Marseille Université 2013. Available from: http://www.theses.fr/2013AIXM4008
19.
Maarifi, Ghizlane.
Rôle de SUMO (Small Ubiquitin-like Modifier protein) dans la réponse à l'interféron et la défense antivirale : Role of SUMO (Small Ubiquitin-like Modifier Protein) in IFN Response and Antiviral Defense.
Degree: Docteur es, Aspects moléculaires et cellulaires de la biologie, 2016, Université Paris-Saclay (ComUE)
URL: http://www.theses.fr/2016SACLS135
► La SUMOylation est une modification post-traductionnelle qui gouverne divers processus cellulaires incluant immunité innée et défense antivirale. Des effecteurs de la synthèse d’IFN, de son…
(more)
▼ La SUMOylation est une modification post-traductionnelle qui gouverne divers processus cellulaires incluant immunité innée et défense antivirale. Des effecteurs de la synthèse d’IFN, de son signal de transduction ainsi que des facteurs de restriction sont modifiés par SUMO (Small Ubiquitin Modifier). Par ailleurs, certains virus exploitent la machinerie SUMO afin de contrecarrer les mécanismes de défense antivirale suggérant l’implication de SUMO dans l’interface virus et défense antivirale. A l’aide d’un modèle cellulaire exprimant les différents paralogues SUMO1, SUMO2 ou SUMO3 ou en diminuant l’expression de l’unique enzyme de conjugaison à SUMO, Ubc9, nous avons montré un effet différentiel de SUMO sur deux virus de la famille des Rhabdoviridae (virus de la stomatite vésiculaire (VSV) et le virus de la rage) et sur la réponse aux IFN alpha et IFN gamma. Le premier axe de recherche a permis de montrer que l’expression de SUMO inhibe la synthèse de l’IFN suite à l’infection par le VSV et le virus de la rage, rendant les cellules plus sensibles à l’infection par le virus de la rage. IRF3 est conjuguée à SUMO, ce qui corrèle avec l’inhibition de sa phosphorylation et l’inhibition de la synthèse d’IFN beta. En revanche, bien que la synthèse de l'IFN soit diminuée, l’expression de SUMO confère la résistance au VSV et inhibe sa transcription primaire. L’activité anti-VSV de SUMO est abolie par la déplétion de MxA. L’effet de SUMO est médié par sa capacité à augmenter l’oligomérisation et la stabilité de MxA. Par ailleurs, ce travail a permis d’identifier MxA comme nouvelle cible de la machinerie SUMO. MxA interagit avec SUMO de manière covalente sur la lysine K48 et de manière non covalente avec SUMO1. Le second axe de recherche a permis d’identifier SUMO comme un nouveau régulateur de la réponse aux IFN. La SUMOylation de STAT1 inhibe sa phosphorylation, régulant négativement le signal de transduction et par conséquent la transcription et la réponse biologique en réponse à l’IFN gamma. En revanche, l’expression de SUMO n’altère ni le signal de transduction ni la transcription en réponse à l’IFN alpha.Par ailleurs, dans les cellules exprimant SUMO3, l’IFN gamma et l’IFN alpha induisent la SUMOylation de PML par SUMO3 ce qui entraîne sa dégradation via le protéasome et inhibe les réponses biologiques médiées par PML. Ce travail a permis de montrer un rôle central de SUMO dans l’immunité intrinsèque et innée, médié par la SUMOylation de protéines cellulaires telles qu’IRF3, MxA, STAT1 ou PML.
SUMOylation modulates several cellular process including innate immunity and antiviral defense. Many key regulators involved in IFN induction, IFN signaling as well as various restriction factors are SUMOylated. Using cells stably expressing the different paralogs of SUMO; SUMO1, SUMO2 or SUMO3 and cells depleted of the only known SUMO conjugating enzyme, Ubc9, we show a differential effect on two viruses from Rhabdoviridae family (Vesicular Stomatitis Virus (VSV) and rabies virus) and on the response to IFN alpha and IFN gamma.…
Advisors/Committee Members: Chelbi-Alix, Mounira (thesis director), Nisole, Sébastien (thesis director).
Subjects/Keywords: Ifn; Sumo; Stat; Pml; MxA; Rhabdoviridae; Ifn; Sumo; Stat; Pml; MxA; Rhabdoviridae
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APA ·
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APA (6th Edition):
Maarifi, G. (2016). Rôle de SUMO (Small Ubiquitin-like Modifier protein) dans la réponse à l'interféron et la défense antivirale : Role of SUMO (Small Ubiquitin-like Modifier Protein) in IFN Response and Antiviral Defense. (Doctoral Dissertation). Université Paris-Saclay (ComUE). Retrieved from http://www.theses.fr/2016SACLS135
Chicago Manual of Style (16th Edition):
Maarifi, Ghizlane. “Rôle de SUMO (Small Ubiquitin-like Modifier protein) dans la réponse à l'interféron et la défense antivirale : Role of SUMO (Small Ubiquitin-like Modifier Protein) in IFN Response and Antiviral Defense.” 2016. Doctoral Dissertation, Université Paris-Saclay (ComUE). Accessed January 18, 2021.
http://www.theses.fr/2016SACLS135.
MLA Handbook (7th Edition):
Maarifi, Ghizlane. “Rôle de SUMO (Small Ubiquitin-like Modifier protein) dans la réponse à l'interféron et la défense antivirale : Role of SUMO (Small Ubiquitin-like Modifier Protein) in IFN Response and Antiviral Defense.” 2016. Web. 18 Jan 2021.
Vancouver:
Maarifi G. Rôle de SUMO (Small Ubiquitin-like Modifier protein) dans la réponse à l'interféron et la défense antivirale : Role of SUMO (Small Ubiquitin-like Modifier Protein) in IFN Response and Antiviral Defense. [Internet] [Doctoral dissertation]. Université Paris-Saclay (ComUE); 2016. [cited 2021 Jan 18].
Available from: http://www.theses.fr/2016SACLS135.
Council of Science Editors:
Maarifi G. Rôle de SUMO (Small Ubiquitin-like Modifier protein) dans la réponse à l'interféron et la défense antivirale : Role of SUMO (Small Ubiquitin-like Modifier Protein) in IFN Response and Antiviral Defense. [Doctoral Dissertation]. Université Paris-Saclay (ComUE); 2016. Available from: http://www.theses.fr/2016SACLS135
20.
Princz, Andrea.
SUMOylation as a fine-tuner of mitochondrial homeostasis and ageing in Caenorhabditis elegans.
Degree: 2018, University of Crete (UOC); Πανεπιστήμιο Κρήτης
URL: http://hdl.handle.net/10442/hedi/45215
► The insulin/IGF signalling pathway impacts lifespan across distant taxa, by controlling the activity of nodal transcription factors. In the nematode Caenorhabditis elegans, the transcription regulators…
(more)
▼ The insulin/IGF signalling pathway impacts lifespan across distant taxa, by controlling the activity of nodal transcription factors. In the nematode Caenorhabditis elegans, the transcription regulators DAF-16/FOXO and SKN-1/Nrf function to promote longevity under conditions of low insulin/IGF signalling and stress. The activity and sub cellular localization of both DAF-16 and SKN-1 is further modulated by specific posttranslational modifications, such as phosphorylation and ubiquitination. Here, we show that ageing elicits a marked increase of SUMO levels in C. elegans. In turn, SUMO fine-tunes DAF-16 and SKN-1 activity in specific C. elegans somatic tissues, to enhance stress resistance. SUMOylation of DAF-16 modulates mitochondrial homeostasis by interfering with mitochondrial dynamics and mitophagy. Our findings reveal that SUMO is an important determinant of lifespan, and provide novel insight, relevant to the complexity of the signalling mechanisms that influence gene expression to govern organismal survival in metazoans.
Το σηματοδοτικό μονοπάτι της ινσουλίνης/προσομοιάζοντος στην ινσουλίνη αυξητικού παράγοντα επηρεάζει τη διάρκεια της ζωής απομακρυσμένων εξελικτικά τάξεων, ελέγχοντας την δράση μεταγραφικών παραγόντων. Στο νηματώδη Caenorhabditis elegans και σε καταστάσεις χαμηλής σηματοδότησης του μονοπατιού της ινσουλίνης/ προσομοιάζοντος στην ινσουλίνη αυξητικού παράγοντα και στρες, οι μεταγραφικοί παράγοντες DAF-16/FOXO και SKN-1/Nrf επάγουν τη μακροζωία. Η δράση και ο υπο-κυτταρικός εντοπισμός των DAF-16 και SKN-1 καθορίζονται περαιτέρω από συγκεκριμένες μετα-μεταγραφικές τροποποιήσεις, όπως η φωσφορυλίωση και η ουβικουϊτίνωση. Σ’ αυτή τη διδακτορική διατριβή, δείχνουμε ότι η γήρανση προκαλεί μια αξιοσημείωτη αύξηση στα επίπεδα της πρωτεϊνης SUMO στον C. elegans. Με τη σειρά της, η SUMO συντονίζει τη δράση των DAF-16 και SKN-1 σε συγκεκριμένους ιστούς του νηματώδη, ώστε να ενισχύσει την αντοχή στο στρες. Η σουμοϋλίωση του DAF-16 διαμορφώνει την ομοιόσταση των μιτοχονδρίων παρεμβαίνοντας στη μιτοχονδριακή δυναμική και τη μιτοφαγία. Τα ευρήματά μας αποκαλύπτουν ότι η SUMO είναι ένας σημαντικός ρυθμιστής της διάρκειας ζωής και παρέχει νέες ιδέες σχετικά με την πολυπλοκότητα των σηματοδοτικών μηχανισμών οι οποίοι επηρεάζουν την γονιδιακή έκφραση ώστε να καθορίσουν την επιβίωση του οργανισμού στα μετάζωα.
Subjects/Keywords: Γήρανση; Μιτοχόνδρια; SUMO; DAF-16; SKN-1; Ageing; Mitochondria; SUMO; DAF-16; SKN-1
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APA (6th Edition):
Princz, A. (2018). SUMOylation as a fine-tuner of mitochondrial homeostasis and ageing in Caenorhabditis elegans. (Thesis). University of Crete (UOC); Πανεπιστήμιο Κρήτης. Retrieved from http://hdl.handle.net/10442/hedi/45215
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Princz, Andrea. “SUMOylation as a fine-tuner of mitochondrial homeostasis and ageing in Caenorhabditis elegans.” 2018. Thesis, University of Crete (UOC); Πανεπιστήμιο Κρήτης. Accessed January 18, 2021.
http://hdl.handle.net/10442/hedi/45215.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Princz, Andrea. “SUMOylation as a fine-tuner of mitochondrial homeostasis and ageing in Caenorhabditis elegans.” 2018. Web. 18 Jan 2021.
Vancouver:
Princz A. SUMOylation as a fine-tuner of mitochondrial homeostasis and ageing in Caenorhabditis elegans. [Internet] [Thesis]. University of Crete (UOC); Πανεπιστήμιο Κρήτης; 2018. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/10442/hedi/45215.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Princz A. SUMOylation as a fine-tuner of mitochondrial homeostasis and ageing in Caenorhabditis elegans. [Thesis]. University of Crete (UOC); Πανεπιστήμιο Κρήτης; 2018. Available from: http://hdl.handle.net/10442/hedi/45215
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universitat Autònoma de Barcelona
21.
Liu, Bing.
Structure insights into the autoinhibitory mechanism of the deubiquitinating enzyme USP25 and into the SUMO E1-E2 protein-protein recognition.
Degree: Departament de Bioquímica i Biologia Molecular, 2018, Universitat Autònoma de Barcelona
URL: http://hdl.handle.net/10803/665314
► Ubiquitination and SUMOylation are of the most studied post-translational modifications (PTMs). Here, we focus on USP25, USP28, and the SUMO E1-E2 protein-protein recognition in these…
(more)
▼ Ubiquitination and SUMOylation are of the most studied post-translational modifications (PTMs). Here, we focus on USP25, USP28, and the
SUMO E1-E2 protein-protein recognition in these two PTM pathways. USP25 and USP28 have important roles in cellular processes, and their enzymatic activities are regulated by diverse PTMs including SUMOylation, ubiquitination, and phosphorylation.
SUMO E1-E2 protein-protein interaction is a major discrimination step in the conjugation pathway. In this thesis, the main goals include the elucidation of the structural basis for the activity regulation of USP25 and USP28, as well as to decipher the structural determinants for the specificity provided by the E1 UFD-E2 interaction.
We have solved the crystal structure of human USP25 (18 – 714). Unexpectedly, USP25 displays a homotetrameric quaternary assembly that is directly involved in the inhibition of its enzymatic activity, revealing a novel tetramerization/inhibition mechanism. In vitro biochemical and kinetic assays with dimer, tetramer and truncation constructs of USP25 support this mechanism, displaying in all cases a higher catalytic activity in the dimer assembly. Moreover, the strong stabilization of tankyrases in cultured cells by the ectopic expression of the USP25 dimer verifies the biological relevance of this novel tetramerization/inhibition mechanism.
Regarding to the E1 UFD-E2 interaction, we have solved the crystal structure of the E1 UFD-E2 complex in both human and A. thaliana. Despite the low sequence homology displayed by the UFD binding interface, structural comparison between complexes reveals common determinants in the interfaces between human, yeast, and A. thaliana. Structural comparison also reveals a strong conservation in the E2 binding interface across species, despite the strong specificity displayed in
SUMO conjugation assays for each organism. Interestingly, E2 residues outside the UFD interface had impact on
SUMO conjugation, suggesting the contribution of determinants other than the primary UFD binding interface in the specificity of the conjugation system.
Advisors/Committee Members: [email protected] (authoremail), true (authoremailshow), Reverter Cendrós, David (director).
Subjects/Keywords: Enzim deubiquitina; Enzima deubiquitina; Deubiquitinating enzyme; Enzim conjugador de SUMO; Enzima conjugador de SUMO; SUMO conjugating ezyme; Estructutra de proteïnes; Estrutura de proteínas; Protein strcuture; Ciències Experimentals; 577
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APA ·
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CSE |
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APA (6th Edition):
Liu, B. (2018). Structure insights into the autoinhibitory mechanism of the deubiquitinating enzyme USP25 and into the SUMO E1-E2 protein-protein recognition. (Thesis). Universitat Autònoma de Barcelona. Retrieved from http://hdl.handle.net/10803/665314
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Liu, Bing. “Structure insights into the autoinhibitory mechanism of the deubiquitinating enzyme USP25 and into the SUMO E1-E2 protein-protein recognition.” 2018. Thesis, Universitat Autònoma de Barcelona. Accessed January 18, 2021.
http://hdl.handle.net/10803/665314.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Liu, Bing. “Structure insights into the autoinhibitory mechanism of the deubiquitinating enzyme USP25 and into the SUMO E1-E2 protein-protein recognition.” 2018. Web. 18 Jan 2021.
Vancouver:
Liu B. Structure insights into the autoinhibitory mechanism of the deubiquitinating enzyme USP25 and into the SUMO E1-E2 protein-protein recognition. [Internet] [Thesis]. Universitat Autònoma de Barcelona; 2018. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/10803/665314.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Liu B. Structure insights into the autoinhibitory mechanism of the deubiquitinating enzyme USP25 and into the SUMO E1-E2 protein-protein recognition. [Thesis]. Universitat Autònoma de Barcelona; 2018. Available from: http://hdl.handle.net/10803/665314
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Indian Institute of Science
22.
Rai, Ragini.
Molecular Genetic Analysis Of The Role Of Nse2, A SUMO E3 Ligase Of The Smc5/6 Complex, In Resisting Genotoxic Stress And Maintaining Chromosome Stability In Saccharomyces Cerevisiae.
Degree: PhD, Faculty of Science, 2011, Indian Institute of Science
URL: http://etd.iisc.ac.in/handle/2005/1020
► DNA repair pathways have evolved to protect the genome from damage caused by intrinsic and extrinsic factors. Although numerous DNA repair mechanisms have been studied…
(more)
▼ DNA repair pathways have evolved to protect the genome from damage caused by intrinsic and extrinsic factors. Although numerous DNA repair mechanisms have been studied and reported, information regarding how they coordinate with the necessary changes in chromatin structure is scarce. Smc (structural maintenance of chromosomes) proteins are a conserved, essential family of proteins required for chromosome organization and accurate segregation. The budding yeast, Saccharomyces cerevisiae has three Smc-protein complexes: Smc1/3 complex (cohesin), Smc2/4 complex (condensin) and the Smc5/6 complex, required for sister chromatid cohesion, condensation and DNA repair, respectively. The chromatin associated Smc5/6 complex consists of Smc5, Smc6 and six non-smc elements (Nse1-Nse6). Smc5 and Smc6 are required for stability of repetitive chromosomal regions and sister chromatid recombination-mediated repair of double-strand breaks.
Mms21/Nse2, a subunit of the Smc5/6 complex, is a
SUMO E3-ligase, which conjugates
SUMO (small ubiquitin-like modifier) to Smc5 and Yku70 (DNA repair protein) and its
SUMO ligase activity protects the cells from extrinsic DNA damage. To address the role of Nse2
SUMO ligase in cellular events, we isolated mutants (nse2∆sl and nse2C221A) defective in the E3-ligase domain of Nse2 and found that these mutants are sensitive to genotoxic agents, for example MMS, UV or bleomycin, as expected. We found that cysteine 221 present in the SP-RING domain of Nse2 is required in the function of Nse2 in resisting genotoxic stress. We found that nse2∆sl cultures are slow growing and show increased abundance of cells having 2N DNA content (indicative of a G2-M cell cycle delay or arrest) relative to wild type cells. The DNA damage checkpoint pathway is activated to a limited extent in unchallenged nse2∆sl mutant cells indicating that cells lacking the
SUMO ligase activity of Nse2 incur spontaneous DNA damage. Furthermore nse2∆sl cells are exquisitely sensitive to caffeine, an agent known to override the DNA damage checkpoint in a number of organisms by inhibiting the DNA damage checkpoint transducer ATR (Homo sapiens), Mec1 (Saccharomyces cerevisiae) and Rad3 (Schizosaccharomyces pombe). In order to investigate the importance of the DNA damage checkpoint pathway for nse2∆sl cells, we employed a genetic approach. We found that nse2∆sl exhibits synthetic sick interaction with mec1∆ but not tel1∆ (defective in Mec1 or Tel1 PI kinases) or mrc1∆ (defective in Mrc1 or mediator of replication checkpoint 1) indicating that the DNA damage induced Mec1 dependent checkpoint pathway is selectively required but the replication stress checkpoint pathway is dispensable for optimal growth of unchallenged nse2∆sl cells.
In order to further investigate the role of Nse2 in S phase events, we used camptothecin (CPT), a drug that induces S phase specific double strand breaks. CPT inhibits topoisomerase I by trapping the covalent Top1-DNA intermediate. Collision of a DNA replication fork with such a complex results in double-strand and…
Advisors/Committee Members: Laloraya, Shikha (advisor).
Subjects/Keywords: Chomosome; Molecular Genetics; Sumo E3 Ligase; Saccharomyces Cerevisiae; Sumolyation; Nse2; SUMO E3; Smc5 Complex; Smc6 Complex; Biochemical Genetics
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APA ·
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APA (6th Edition):
Rai, R. (2011). Molecular Genetic Analysis Of The Role Of Nse2, A SUMO E3 Ligase Of The Smc5/6 Complex, In Resisting Genotoxic Stress And Maintaining Chromosome Stability In Saccharomyces Cerevisiae. (Doctoral Dissertation). Indian Institute of Science. Retrieved from http://etd.iisc.ac.in/handle/2005/1020
Chicago Manual of Style (16th Edition):
Rai, Ragini. “Molecular Genetic Analysis Of The Role Of Nse2, A SUMO E3 Ligase Of The Smc5/6 Complex, In Resisting Genotoxic Stress And Maintaining Chromosome Stability In Saccharomyces Cerevisiae.” 2011. Doctoral Dissertation, Indian Institute of Science. Accessed January 18, 2021.
http://etd.iisc.ac.in/handle/2005/1020.
MLA Handbook (7th Edition):
Rai, Ragini. “Molecular Genetic Analysis Of The Role Of Nse2, A SUMO E3 Ligase Of The Smc5/6 Complex, In Resisting Genotoxic Stress And Maintaining Chromosome Stability In Saccharomyces Cerevisiae.” 2011. Web. 18 Jan 2021.
Vancouver:
Rai R. Molecular Genetic Analysis Of The Role Of Nse2, A SUMO E3 Ligase Of The Smc5/6 Complex, In Resisting Genotoxic Stress And Maintaining Chromosome Stability In Saccharomyces Cerevisiae. [Internet] [Doctoral dissertation]. Indian Institute of Science; 2011. [cited 2021 Jan 18].
Available from: http://etd.iisc.ac.in/handle/2005/1020.
Council of Science Editors:
Rai R. Molecular Genetic Analysis Of The Role Of Nse2, A SUMO E3 Ligase Of The Smc5/6 Complex, In Resisting Genotoxic Stress And Maintaining Chromosome Stability In Saccharomyces Cerevisiae. [Doctoral Dissertation]. Indian Institute of Science; 2011. Available from: http://etd.iisc.ac.in/handle/2005/1020
23.
Marques, Ana Teresa Matos Paixão.
Desenvolvimento de um sumo de fruta com adição da macroalga Gelidium corneum, recorrendo à tecnologia HPP: caracterização físico-química, microbiológica e sensorial.
Degree: 2015, Instituto Politécnico de Leiria
URL: http://www.rcaap.pt/detail.jsp?id=oai:iconline.ipleiria.pt:10400.8/2186
► No setor alimentar, o controlo da qualidade dos produtos e dos processos é uma etapa essencial, uma vez que é por este meio que se…
(more)
▼ No setor alimentar, o controlo da qualidade dos produtos e dos processos é uma etapa essencial, uma vez que é por este meio que se avaliam os padrões exigidos, quer a nível de legislação, quer a nível de mercado.
O presente trabalho teve, como objetivos principais, o desenvolvimento de um novo produto alimentar com adição de algas, bem como o controlo de qualidade a nível de uma unidade fabril de produção de sumos e polpas naturais, à base de hortofrutícolas.
De forma a garantir a qualidade na obtenção de produtos, foi realizado, diariamente, o controlo de entradas dos produtos hortofrutícolas, o acompanhamento de processos nas linhas de produção da fábrica e a análise das amostras de referência.
Considerando a tendência e potencial de crescimento apresentados pelo setor das bebidas, nomeadamente dos sumos de fruta naturais, juntamente com a incessante procura por ingredientes naturais ricos em vários nutrientes e com propriedades bioativas, tem-se vindo a registar o lançamento de um número considerável de bebidas inovadoras, onde as macroalgas têm merecido um papel de destaque.
Desta forma, procedeu-se à elaboração de dois protótipos, nomeadamente,
sumo de framboesa e
sumo de espinafres, com a adição do extrato da alga Gelidium corneum, estabilizados através do processo de Hiperpressão a frio, com o intuito de se avaliar o impacto desse extrato nos diferentes parâmetros de qualidade dos sumos.
No que respeita aos parâmetros físico-químicos, o pH e teor de sólidos solúveis (TSS) foram avaliados após produção (t0), no tempo intermédio (t15) e no tempo final (t30). A cor foi determinada no t0 e no t30 e os restantes parâmetros, como acidez titulável, quantificação total de polifenóis, capacidade de redução do radical DPPH, teor de proteína bruta, teor de cinzas, teor de minerais e oligoelementos e teor de vitaminas (A, B1, B2 e C) foram avaliados no tempo final (t30). Foram, também, avaliados os parâmetros microbiológicos mais significativos, nomeadamente, microrganismos totais a 30 ˚C, Escherichia coli, Salmonella spp, bolores e leveduras (ao t0, t15 e t30) e realizou-se uma avaliação sensorial ao 29.º dia após produção.
Estes sumos apresentaram-se microbiologicamente estáveis, não se tendo verificado crescimento microbiológico durante o período de armazenamento de 30 dias.
A análise dos resultados físico-químicos permitiram constatar que a adição do extrato da alga Gelidium corneum aos sumos influenciou as suas características, tendo havido um aumento significativo do pH com a consequente diminuição da acidez titulável e um aumento do teor de sólidos solúveis, em comparação com os sumos controlo. Em relação à avaliação da cor, no caso das amostras de sumos de framboesa, verificou-se que a adição do extrato de algas influenciou o parâmetro a*. No entanto, ambas as amostras permitiram a manutenção deste parâmetro, ao longo do tempo. Quanto ao parâmetro b*, as duas amostras de
sumo com algas demonstraram uma evolução mais estável, em relação aos respetivos controlos. No que respeita ao teor em minerais, a adição do…
Advisors/Committee Members: Bernardino, Susana Maria Silva Agostinho, Raimundo, Carla.
Subjects/Keywords: Processo de Hiperpressão; sumo de framboesa; sumo de espinafres; alga Gelidium corneum; Domínio/Área Científica::Engenharia e Tecnologia
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APA (6th Edition):
Marques, A. T. M. P. (2015). Desenvolvimento de um sumo de fruta com adição da macroalga Gelidium corneum, recorrendo à tecnologia HPP: caracterização físico-química, microbiológica e sensorial. (Thesis). Instituto Politécnico de Leiria. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:iconline.ipleiria.pt:10400.8/2186
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Marques, Ana Teresa Matos Paixão. “Desenvolvimento de um sumo de fruta com adição da macroalga Gelidium corneum, recorrendo à tecnologia HPP: caracterização físico-química, microbiológica e sensorial.” 2015. Thesis, Instituto Politécnico de Leiria. Accessed January 18, 2021.
http://www.rcaap.pt/detail.jsp?id=oai:iconline.ipleiria.pt:10400.8/2186.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Marques, Ana Teresa Matos Paixão. “Desenvolvimento de um sumo de fruta com adição da macroalga Gelidium corneum, recorrendo à tecnologia HPP: caracterização físico-química, microbiológica e sensorial.” 2015. Web. 18 Jan 2021.
Vancouver:
Marques ATMP. Desenvolvimento de um sumo de fruta com adição da macroalga Gelidium corneum, recorrendo à tecnologia HPP: caracterização físico-química, microbiológica e sensorial. [Internet] [Thesis]. Instituto Politécnico de Leiria; 2015. [cited 2021 Jan 18].
Available from: http://www.rcaap.pt/detail.jsp?id=oai:iconline.ipleiria.pt:10400.8/2186.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Marques ATMP. Desenvolvimento de um sumo de fruta com adição da macroalga Gelidium corneum, recorrendo à tecnologia HPP: caracterização físico-química, microbiológica e sensorial. [Thesis]. Instituto Politécnico de Leiria; 2015. Available from: http://www.rcaap.pt/detail.jsp?id=oai:iconline.ipleiria.pt:10400.8/2186
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Université Paris-Sud – Paris XI
24.
Maroui, Mohamed Ali.
Rôle et devenir de PML lors de l’infection par l’EMCV : Role and fate of PML during EMCV infection.
Degree: Docteur es, Biochimie, Biologie cellulaire et moléculaire, 2012, Université Paris-Sud – Paris XI
URL: http://www.theses.fr/2012PA11T008
► PML et les corps nucléaires (CN) sont impliqués dans la défense antivirale. En effet, notre équipe a montré que la surexpression de PMLIII confère la…
(more)
▼ PML et les corps nucléaires (CN) sont impliqués dans la défense antivirale. En effet, notre équipe a montré que la surexpression de PMLIII confère la résistance au virus de la stomatite vésiculaire, au virus de l'influenza, au virus foamy mais pas au virus de l’encéphalomyocardite (EMCV). J’ai montré dans mon travail de thèse que l’EMCV contrecarre le pouvoir antiviral de PMLIII en induisant sa dégradation par un processus dépendant du protéasome et de SUMO. Cependant, les cellules de souris invalidées pour PML sont plus sensibles à l’infection par l’EMCV que les cellules issues de souris parentales. Pour déterminer l’isoforme de PML responsable de cet effet antiviral, j’ai analysé l’effet des sept isoformes de PML (PMLI-VII) et j’ai montré que seule l’expression en stable de PMLIV confère la résistance à l’EMCV en séquestrant la polymérase virale 3Dpol au sein des CN PML. De plus la déplétion de PMLIV augmente la production de l’EMCV dans les cellules traitées par l’interféron. Ces données indiquent le mécanisme par lequel PML confère la résistance à l’EMCV et révèlent que PML est l’une des protéines médiatrices des effets anti-EMCV de l’interféron.
PML and nuclear bodies (NBs) are implicated in antiviral defense. Indeed, our team showed that overexpression of PMLIII confers resistance to vesicular stomatitis virus, influenza virus, foamy virus but not to encephalomyocarditis virus (EMCV). I have shown during my thesis that EMCV counteracts the antiviral effect of PMLIII by inducing its degradation in SUMO and proteasome-dependent way. However, cells derived from PML knockout mice are more susceptible to EMCV infection than wild-type cells. To determine the isoforme of PML implicated in this antiviral effect, I analysed the effect of the seven PML isoforms (PMLI-PMLVII) and I showed that only stable expression of PMLIV confers resistance to EMCV by sequestring the viral polymérase 3Dpol in PML Nbs. In addition, depletion of PMLIV boosted EMCV production in interferon-treated cells. These finding sindicate the mechanism by which PML confers resistance to EMCV and reveal a new pathway mediating the antiviral activity of interferon against EMCV.
Advisors/Committee Members: Chelbi-Alix, Mounira (thesis director).
Subjects/Keywords: Interféron; PML; Corps nucléaires; SUMO; EMCV; 3D polymérase; 3C protéase; Interferon; PML; Nuclear bodies; SUMO; EMCV; 3D polymerase; 3C protease
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APA ·
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MLA ·
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to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Maroui, M. A. (2012). Rôle et devenir de PML lors de l’infection par l’EMCV : Role and fate of PML during EMCV infection. (Doctoral Dissertation). Université Paris-Sud – Paris XI. Retrieved from http://www.theses.fr/2012PA11T008
Chicago Manual of Style (16th Edition):
Maroui, Mohamed Ali. “Rôle et devenir de PML lors de l’infection par l’EMCV : Role and fate of PML during EMCV infection.” 2012. Doctoral Dissertation, Université Paris-Sud – Paris XI. Accessed January 18, 2021.
http://www.theses.fr/2012PA11T008.
MLA Handbook (7th Edition):
Maroui, Mohamed Ali. “Rôle et devenir de PML lors de l’infection par l’EMCV : Role and fate of PML during EMCV infection.” 2012. Web. 18 Jan 2021.
Vancouver:
Maroui MA. Rôle et devenir de PML lors de l’infection par l’EMCV : Role and fate of PML during EMCV infection. [Internet] [Doctoral dissertation]. Université Paris-Sud – Paris XI; 2012. [cited 2021 Jan 18].
Available from: http://www.theses.fr/2012PA11T008.
Council of Science Editors:
Maroui MA. Rôle et devenir de PML lors de l’infection par l’EMCV : Role and fate of PML during EMCV infection. [Doctoral Dissertation]. Université Paris-Sud – Paris XI; 2012. Available from: http://www.theses.fr/2012PA11T008
University of Oxford
25.
Biasutto, Antonio.
Structural insights into human SNF2/SWI2 chromatin remodeler SMARCAD1 and its role in DNA repair.
Degree: PhD, 2016, University of Oxford
URL: http://ora.ox.ac.uk/objects/uuid:efc019ba-41ee-4dbd-adfb-2786017e91aa
;
https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.730334
► ATP-dependent chromatin remodelers have been proposed to act sequentially, and to a certain extent non-redundantly, in the priming stages of the DNA Damage Response pathways…
(more)
▼ ATP-dependent chromatin remodelers have been proposed to act sequentially, and to a certain extent non-redundantly, in the priming stages of the DNA Damage Response pathways by establishing chromatin in lesion sites ready to act as a scaffold for repair factors or to be displaced in order to allow DNA repair. Among remodeling factors proposed to play a role in DNA repair is SMARCAD1, a poorly characterized, non-canonical member of the SWR1-like family of SNF2/SWI2 superfamily of ATPases, which has recently been identified as a potential target for ATM/ATR phosphorylation at canonical and non-canonical sites upon DNA damage. The actual mechanism for SMARCAD1 recruitment and involvement in DNA remodeling is still unknown, and unlike most other chromatin remodelers, SMARCAD1 does not contain DNA- or histone-binding domains frequently accompanying such proteins. Instead, in addition to the core ATPase domain, only two CUE domains (a type of helical ubiquitin-binding domain) have been identified. This thesis presents the findings of an investigation intended to structurally characterize SMARCAD1 by dissecting and identifying its domain architecture, and examining the activity and ligand selectivity of its binding domains in the functional context of DNA damage repair. The solution NMR structure of the CUE1 domain is presented, describing a triple helix bundle consistent with other members of the family. Furthermore, a novel SUMO interacting motif was identified and through a combination of NMR titrations and phospho-proteomics analysis, shown to be constitutively phosphorylated which excludes the possibility of DNA damage dependent ATM targeting as the recruitment mechanism for DNA repair. Additionally, it is demonstrated that both CUE domains are poor binders of mono-ubiquitin, however CUE1 specifically mediates the high affinity binary interaction with the transcriptionally repressive master regulator KAP1. This interaction was shown to be independent of post-translational ubiquitylation but rather sustained through direct interaction with the dimeric RBCC domain of KAP1. Finally, mass spectrometry profiling of domain-dependent interactions (based on differential abundance relative to changes due to chemically induced DNA damage) suggests SMARCAD1 may be involved in p53 transcriptional regulation through interactions maintained with CUE1 prior to DNA damage, whereas the SIM domain selectively targets protein interactions upon DNA damage that simultaneously activate p53 transcriptional control and recruit SMARCAD1 to DNA damage repair pathways.
Subjects/Keywords: 572.8; Biochemistry; NMR Spectroscopy; Chromatin Remodelling; Ubiquitin; nuclear magnetic resonance; SMARCAD1; CUE Domain; SUMO Interacting Motif; Protein Structure; SUMO
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APA (6th Edition):
Biasutto, A. (2016). Structural insights into human SNF2/SWI2 chromatin remodeler SMARCAD1 and its role in DNA repair. (Doctoral Dissertation). University of Oxford. Retrieved from http://ora.ox.ac.uk/objects/uuid:efc019ba-41ee-4dbd-adfb-2786017e91aa ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.730334
Chicago Manual of Style (16th Edition):
Biasutto, Antonio. “Structural insights into human SNF2/SWI2 chromatin remodeler SMARCAD1 and its role in DNA repair.” 2016. Doctoral Dissertation, University of Oxford. Accessed January 18, 2021.
http://ora.ox.ac.uk/objects/uuid:efc019ba-41ee-4dbd-adfb-2786017e91aa ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.730334.
MLA Handbook (7th Edition):
Biasutto, Antonio. “Structural insights into human SNF2/SWI2 chromatin remodeler SMARCAD1 and its role in DNA repair.” 2016. Web. 18 Jan 2021.
Vancouver:
Biasutto A. Structural insights into human SNF2/SWI2 chromatin remodeler SMARCAD1 and its role in DNA repair. [Internet] [Doctoral dissertation]. University of Oxford; 2016. [cited 2021 Jan 18].
Available from: http://ora.ox.ac.uk/objects/uuid:efc019ba-41ee-4dbd-adfb-2786017e91aa ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.730334.
Council of Science Editors:
Biasutto A. Structural insights into human SNF2/SWI2 chromatin remodeler SMARCAD1 and its role in DNA repair. [Doctoral Dissertation]. University of Oxford; 2016. Available from: http://ora.ox.ac.uk/objects/uuid:efc019ba-41ee-4dbd-adfb-2786017e91aa ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.730334
Brno University of Technology
26.
Varmus, Pavol.
Analýza vehicular ad hoc sítě: Analysis of vehicular ad hoc network.
Degree: 2019, Brno University of Technology
URL: http://hdl.handle.net/11012/177615
► This diploma thesis aims to study VANET (vehicular ad hoc network), to describe the theory of this networks and describe attributes of these networks and…
(more)
▼ This diploma thesis aims to study VANET (vehicular ad hoc network), to describe the theory of this networks and describe attributes of these networks and to set the starting point for practical part. Thesis includes VANETs possibilities, its signal transportation and description of routing protocols. Another goal was to familiarize program NS-3 and set up simulation models in its interface. The main output of the practical part is program which simulates vehicle movement in Brno city and set the communication module which is adapted to fulfill the most realistic transmission capabilities. Practical part is divided to two parts. The goal of the first one was to simulate basic communication in theorized unrealistic scenario and the second part was the more realistic scenario. Overall, throughout the practical part was tested a variety of attributes, such as mobility models, standards, routing protocols and other parameters that provided diversity in final results. All the results, which consisted of summary of basic transmission capabilities and reclassification of the applicability of those technologies in real world, are discussed in the summary of the simulations output.
Advisors/Committee Members: Kubánková, Anna (advisor), Mašek, Pavel (referee).
Subjects/Keywords: VANET; smerovacie protokoly; modulácie; infraštruktúra; vozidlá; NS-3; SUMO; VANET; routing protocols; modulations; infrastructure; vehicles NS-3; SUMO
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APA (6th Edition):
Varmus, P. (2019). Analýza vehicular ad hoc sítě: Analysis of vehicular ad hoc network. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/177615
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Varmus, Pavol. “Analýza vehicular ad hoc sítě: Analysis of vehicular ad hoc network.” 2019. Thesis, Brno University of Technology. Accessed January 18, 2021.
http://hdl.handle.net/11012/177615.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Varmus, Pavol. “Analýza vehicular ad hoc sítě: Analysis of vehicular ad hoc network.” 2019. Web. 18 Jan 2021.
Vancouver:
Varmus P. Analýza vehicular ad hoc sítě: Analysis of vehicular ad hoc network. [Internet] [Thesis]. Brno University of Technology; 2019. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/11012/177615.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Varmus P. Analýza vehicular ad hoc sítě: Analysis of vehicular ad hoc network. [Thesis]. Brno University of Technology; 2019. Available from: http://hdl.handle.net/11012/177615
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
27.
Ait Ammar, Amina.
KAP1 : un nouveau facteur répresseur de la transcription du VIH-1 : KAP1 : a new repressor factor of HIV-1 transcription.
Degree: Docteur es, Aspects moléculaires et cellulaires de la biologie, 2018, Université de Strasbourg
URL: http://www.theses.fr/2018STRAJ073
► La latence post-intégrative du VIH-1 génère des réservoirs qui empêchent l'éradication du virus avec les thérapies actuelles. La compréhension des mécanismes moléculaires de la latence…
(more)
▼ La latence post-intégrative du VIH-1 génère des réservoirs qui empêchent l'éradication du virus avec les thérapies actuelles. La compréhension des mécanismes moléculaires de la latence servirait à l'identification de nouvelles cibles thérapeutiques et le développement de molécules de type LRA (Latency Reversing Agent) permettant la guérison fonctionnelle des patients et un arrêt des traitements. Nous avons démontré que la combinaison de deux types différents de LRAs à base de composés libérant P-TEFb (JQ1, I-BET, I-BET151 et HMBA) ou des agonistes de PKC (prostratine, bryostatine-1 et Ing-B) conduit à une robuste activation synergique de la production du VIH-1 dans divers modèles cellulaires de latence post-intégrative et dans des réservoirs de lymphocytes T CD4+ primaires. Le répresseur transcriptionnel CTIP2 recrute des complexes multienzymatiques pour favoriser l'établissement et la persistance de la latence du VIH-1 dans les cellules microgliales, le principal réservoir viral du système nerveux central. De plus, CTIP2 s’associe au complexe 7SK snRNP pour inhiber P-TEFb, un facteur d’élongation indispensable à l’expression du VIH-1 et à la réactivation des provirus latents. Des expériences d’immunoprécipitations de CTIP2, couplées à la spectrométrie de masse, nous ont permis d’identifier près de 900 partenaires protéiques de CTIP2. Parmi ces nouveaux partenaires, nous avons identifié la SUMO E3 ligase KAP1. Nous montrons que KAP1 réprime les phases précoce et tardive Tat dépendante de la transcription du VIH-1. KAP1 induit une dégradation de Tat, qui est sensible aux modulations de la voie SUMO. En effet, la sumoylation favorise l'association de Tat avec KAP1, de même que sa dégradation. Globalement, nos résultats suggèrent que KAP1 contribue à l'établissement et à la persistance des réservoirs latents du VIH-1. Cibler les voies SUMO constituerait un nouveau champ d'investigation dans le cadre du développement de nouvelles classes de LRAs.
The HIV-1 post-integration latency generates reservoirs that prevent the eradication of the virus with the current therapies. The understanding of the molecular mechanisms of this latency enable the identification of new therapeutic targets and the development of LRA (Latency Reversing Agent) for functional cure and treatments interruption. We have demonstrated that the combination of two different LRAs based on P-TEFb releasing compounds (JQ1, I-BET, I- BET151 and HMBA) or PKC agonists (prostratin, bryostatin-1 and Ing-B) leads to robust synergistic activation of HIV-1 production in various cellular models of post-integration latency and in primary CD4+ T cells reservoirs. The transcriptional repressor CTIP2 recruits multienzymatic complexes to promote the establishment and persistence of HIV-1 latency in microglial cells, the main viral reservoir of the central nervous system. Furthermore, CTIP2 binds to the 7SK snRNP complex to inhibit P-TEFb, an elongation factor essential for HIV-1 expression and reactivation of latent proviruses. Immunoprecipitation experiments of CTIP2…
Advisors/Committee Members: Rohr, Olivier (thesis director), Gautier, Virginie (thesis director).
Subjects/Keywords: VIH-1; Latence; CTIP2; P-TEFb; KAP1; SUMO; HIV-1; Latency; CTIP2; P-TEFb; KAP1; SUMO; 579.2; 572.8
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APA ·
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APA (6th Edition):
Ait Ammar, A. (2018). KAP1 : un nouveau facteur répresseur de la transcription du VIH-1 : KAP1 : a new repressor factor of HIV-1 transcription. (Doctoral Dissertation). Université de Strasbourg. Retrieved from http://www.theses.fr/2018STRAJ073
Chicago Manual of Style (16th Edition):
Ait Ammar, Amina. “KAP1 : un nouveau facteur répresseur de la transcription du VIH-1 : KAP1 : a new repressor factor of HIV-1 transcription.” 2018. Doctoral Dissertation, Université de Strasbourg. Accessed January 18, 2021.
http://www.theses.fr/2018STRAJ073.
MLA Handbook (7th Edition):
Ait Ammar, Amina. “KAP1 : un nouveau facteur répresseur de la transcription du VIH-1 : KAP1 : a new repressor factor of HIV-1 transcription.” 2018. Web. 18 Jan 2021.
Vancouver:
Ait Ammar A. KAP1 : un nouveau facteur répresseur de la transcription du VIH-1 : KAP1 : a new repressor factor of HIV-1 transcription. [Internet] [Doctoral dissertation]. Université de Strasbourg; 2018. [cited 2021 Jan 18].
Available from: http://www.theses.fr/2018STRAJ073.
Council of Science Editors:
Ait Ammar A. KAP1 : un nouveau facteur répresseur de la transcription du VIH-1 : KAP1 : a new repressor factor of HIV-1 transcription. [Doctoral Dissertation]. Université de Strasbourg; 2018. Available from: http://www.theses.fr/2018STRAJ073
28.
Ristic, Marko.
ROS/SUMO relationship in the chemotherapeutic treatment of Acute Myeloid Leukemia : Relations ROS/Sumoylation au cours des traitements chimiothérapeutiques des Leucémies Aigues Myéloïdes.
Degree: Docteur es, Biologie Santé, 2015, Montpellier
URL: http://www.theses.fr/2015MONTT047
► Les leucémies aiguë myéloïde (LAM) sont un groupe d’hémopathies malignes, dont le traitement est généralement composé de deux génotoxiques : la cytarabine (Ara-C) et la…
(more)
▼ Les leucémies aiguë myéloïde (LAM) sont un groupe d’hémopathies malignes, dont le traitement est généralement composé de deux génotoxiques : la cytarabine (Ara-C) et la daunorubicine (DNR). Nous avons montré que l’Ara-C et la DNR induisent la déconjugaison rapide de SUMO (Small Ubiquitin-related Modifier) de ses protéines cibles. Cette deSUMOylation est dûe à l'inactivation des enzymes E1 et E2 de SUMOylation par les espèces réactives de l'oxygène (ROS) produites par l’Ara-C et la DNR et est impliquée dans l'activation de l'apoptose. En outre, cet axe ROS/SUMO est anergisé dans les LAM chimiorésistantes. Cependant, il peut être réactivé par des pro-oxydants ou par inhibition de la voie SUMO par l'acide anacardique. Pour identifier les protéines contrôlées par l’axe ROS/SUMO nous avons effectué une approche de spectrométrie de masse quantitative (SILAC). Parmi les 1000 protéines SUMOylées identifiées, la plupart des 114 protéines qui perdent leur SUMOylation lors du traitement sont impliquées dans la régulation de l'expression des gènes. De plus, un ChIP-Seq avec des anticorps anti SUMO-2 a permis de montrer que les génotoxiques, en particulier la DNR, induisent une diminution massive de la présence de protéines SUMOylées sur la chromatine. La recherche de motifs au sein des séquences fixant SUMO a permis d’identifier le motif de liaison de CTCF à l’ADN. De plus, CTCF a été trouvé dans la SILAC comme l’une des protéines déSUMOylées par les traitements. En utilisant des données publiques de Chip-Seq pour CTCF, nous avons identifié 55 gènes qui fixent à la fois CTCF et SUMO et dont l’expression est régulée par les traitements. Dans la dernière partie de ce travail, nous avons étudié le groupe de 19 protéines dont la SUMOylation augmente suite aux traitements génotoxiques. Parmi ces protéines, nous avons trouvé diverses protéines centromériques, y compris CENP-B et CENP-C. En utilisant le PLA (Proximity Ligation Assay) nous avons pu montrer que CENP-B et CENP-C colocalisent avec SUMO et yH2AX après traitement. Cela suggère que la SUMOylation des protéines centromériques se produit sur les sites de cassure et pourrait jouer un rôle dans la réparation des dommages de l'ADN.
Acute Myeloid Leukemias (AML) are a group a severe hematological malignancies, which treatment is generally composed of two genotoxics: Cytarabine (Ara-C) and Daunorubicin (DNR). We have shown that these drugs induce the rapid deconjugation of the Small Ubiquitin-related Modifier (SUMO) from its target protein. This is due to the inactivation of SUMO E1 and E2 enzymes by Reactive oxygen species (ROS). This deSUMOylation participated in the activation of specific genes and is involved the induction of apoptosis. In addition, this ROS/SUMO axis is anergized in chemoresistant AMLs. However, it can be reactivated by pro-oxidants or inhibition of the SUMO pathway with anacardic acid, an inhibitor of the SUMO E1. To identify which proteins are regulated by this ROS/SUMO axis, we performed a quantitative mass spectrometry approach. Among the 1000 identified…
Advisors/Committee Members: Piechaczyk, Marc (thesis director).
Subjects/Keywords: Sumo; Leucémie Aigue Myéloide; Chimiothérapie; Espèces oxygénées réactives; Sumo; Acute Myeloid Leukemia; Chemotherapy; Reactive oxigen species
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APA ·
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APA (6th Edition):
Ristic, M. (2015). ROS/SUMO relationship in the chemotherapeutic treatment of Acute Myeloid Leukemia : Relations ROS/Sumoylation au cours des traitements chimiothérapeutiques des Leucémies Aigues Myéloïdes. (Doctoral Dissertation). Montpellier. Retrieved from http://www.theses.fr/2015MONTT047
Chicago Manual of Style (16th Edition):
Ristic, Marko. “ROS/SUMO relationship in the chemotherapeutic treatment of Acute Myeloid Leukemia : Relations ROS/Sumoylation au cours des traitements chimiothérapeutiques des Leucémies Aigues Myéloïdes.” 2015. Doctoral Dissertation, Montpellier. Accessed January 18, 2021.
http://www.theses.fr/2015MONTT047.
MLA Handbook (7th Edition):
Ristic, Marko. “ROS/SUMO relationship in the chemotherapeutic treatment of Acute Myeloid Leukemia : Relations ROS/Sumoylation au cours des traitements chimiothérapeutiques des Leucémies Aigues Myéloïdes.” 2015. Web. 18 Jan 2021.
Vancouver:
Ristic M. ROS/SUMO relationship in the chemotherapeutic treatment of Acute Myeloid Leukemia : Relations ROS/Sumoylation au cours des traitements chimiothérapeutiques des Leucémies Aigues Myéloïdes. [Internet] [Doctoral dissertation]. Montpellier; 2015. [cited 2021 Jan 18].
Available from: http://www.theses.fr/2015MONTT047.
Council of Science Editors:
Ristic M. ROS/SUMO relationship in the chemotherapeutic treatment of Acute Myeloid Leukemia : Relations ROS/Sumoylation au cours des traitements chimiothérapeutiques des Leucémies Aigues Myéloïdes. [Doctoral Dissertation]. Montpellier; 2015. Available from: http://www.theses.fr/2015MONTT047
29.
Boulanger, Mathias.
SUMOylation et contrôle de l’expression des gènes : implication dans la réponse à la chimiothérapie d’induction des Leucémies Aigües Myéloïdes : SUMOylation and control of gene expression : involvement in Acute Myeloid Leukemia response to induction chemotherapy.
Degree: Docteur es, Biologie Santé, 2019, Montpellier
URL: http://www.theses.fr/2019MONTT067
► Les Leucémies Aigues Myéloïdes (LAM) sont des hémopathies malignes au pronostic sombre. Leur traitement repose sur une chimiothérapie composée d’une anthracycline (daunorubicine [DNR] ou idarubicine)…
(more)
▼ Les Leucémies Aigues Myéloïdes (LAM) sont des hémopathies malignes au pronostic sombre. Leur traitement repose sur une chimiothérapie composée d’une anthracycline (daunorubicine [DNR] ou idarubicine) et d’un analogue de nucléotide (AraC). Cependant, les taux de rechutes sont très élevés. Il est donc critique de mieux comprendre les mécanismes d’actions de ces drogues chimiothérapeutiques pour mieux surmonter la chimiorésistance. Un aspect, essentiel à leur action thérapeutique mais encore mal connu, concerne leur capacité à réguler l’expression de gènes spécifiques qui participent à leur effet thérapeutique. Ma thèse a pour objectif de déterminer l’implication de la SUMOylation, une modification post-traductionnelle de la famille de l’ubiquitine, dans la reprogrammation transcriptionnelle induite par les chimiothérapies dans les LAM. La combinaison d’approches génomiques (ChIP-Seq), de transcriptomiques et de protéomiques a montré que la DNR induit une reprogrammation transcriptionnelle importante et rapide de gènes impliqués dans la régulation de l’inflammation et l’apoptose, précédée par une désumoylation massive des protéines présentes sur les promoteurs des gènes dans les LAM. En particulier, j’ai montré que la diminution de CTCF et de SUMO sur le promoteur du gène NFkB2 régulent son induction transcriptionnelle par la DNR, en particulier en favorisant un changement de la structure 3D de son locus.
Acute Myeloid Leukemias (AML) are severe hematological malignancies. Their treatment is based on an intensive chemotherapy composed of one anthracycline (daunorubicin-DNR or Idarubicin) and a nucleotide analog (Ara-C). However, the relapse rates are very high and the prognosis bad. It is therefore critical to better understand their modes of action to overcome chemoresistance. One aspect, essential to their action but still poorly understood, concerns their ability to regulate the expression of specific genes, which participate in their therapeutic potential. The objective of my thesis was to determine the role of SUMOylation, a post-translational modifier of the ubiquitin family, in the DNR-induced transcriptional reprogramming in AML. Genomic approaches (ChIP-seq) were combined with transcriptomics and quantitative proteomic to show that DNR induces a fast and large transcriptomic reprogramming of genes involved in apoptosis and inflammation regulation, which is preceded by promoter-bound protein deSUMOylation. In particular, I could show that the decreased binding of CTCF and SUMO on the promoter of NFkB2 regulates its DNR-induced expression through a remodeling of the 3D structure of its locus.
Advisors/Committee Members: Bossis, Guillaume (thesis director).
Subjects/Keywords: Sumo; Transcription; Leucémies; Ctcf; Dnr; Structure 3D de la chromatine; Sumo; Transcription; Leukemia; Ctcf; Dnr; Chromatin 3D structure
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APA ·
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APA (6th Edition):
Boulanger, M. (2019). SUMOylation et contrôle de l’expression des gènes : implication dans la réponse à la chimiothérapie d’induction des Leucémies Aigües Myéloïdes : SUMOylation and control of gene expression : involvement in Acute Myeloid Leukemia response to induction chemotherapy. (Doctoral Dissertation). Montpellier. Retrieved from http://www.theses.fr/2019MONTT067
Chicago Manual of Style (16th Edition):
Boulanger, Mathias. “SUMOylation et contrôle de l’expression des gènes : implication dans la réponse à la chimiothérapie d’induction des Leucémies Aigües Myéloïdes : SUMOylation and control of gene expression : involvement in Acute Myeloid Leukemia response to induction chemotherapy.” 2019. Doctoral Dissertation, Montpellier. Accessed January 18, 2021.
http://www.theses.fr/2019MONTT067.
MLA Handbook (7th Edition):
Boulanger, Mathias. “SUMOylation et contrôle de l’expression des gènes : implication dans la réponse à la chimiothérapie d’induction des Leucémies Aigües Myéloïdes : SUMOylation and control of gene expression : involvement in Acute Myeloid Leukemia response to induction chemotherapy.” 2019. Web. 18 Jan 2021.
Vancouver:
Boulanger M. SUMOylation et contrôle de l’expression des gènes : implication dans la réponse à la chimiothérapie d’induction des Leucémies Aigües Myéloïdes : SUMOylation and control of gene expression : involvement in Acute Myeloid Leukemia response to induction chemotherapy. [Internet] [Doctoral dissertation]. Montpellier; 2019. [cited 2021 Jan 18].
Available from: http://www.theses.fr/2019MONTT067.
Council of Science Editors:
Boulanger M. SUMOylation et contrôle de l’expression des gènes : implication dans la réponse à la chimiothérapie d’induction des Leucémies Aigües Myéloïdes : SUMOylation and control of gene expression : involvement in Acute Myeloid Leukemia response to induction chemotherapy. [Doctoral Dissertation]. Montpellier; 2019. Available from: http://www.theses.fr/2019MONTT067
Technical University of Lisbon
30.
Oliveira, Pedro Miguel Rodrigues.
Utilização de leveduras na produção de novas bebidas fermentadas.
Degree: 2009, Technical University of Lisbon
URL: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/1093
► Mestrado em Engenharia Alimentar - Instituto Superior de Agronomia
The thesis main objective was to produce new and stable fermented sparkling beverages from fruit and…
(more)
▼ Mestrado em Engenharia Alimentar - Instituto Superior de Agronomia
The thesis main objective was to produce new and stable fermented sparkling beverages from fruit and infusions/teas, with yeast directed for gourmet restaurants. Ten fruits and eighteen infusions/teas were evaluated for organoleptic properties to choose the best potentially four sparkling beverages. Beverages were characterized for physico-chemical composition, pH, total acidity, ºbrix, soluble protein and phenolic compounds. Antimutagenic potential was analyzed by Ames test. Product shelf-life was established (15 days – juices; 30 days - infusions, at 4ºC) and microbiology analyses were done during the same period, while pH, ºbrix, total acidity, glucose, ethanol and pressure were monitored. RAPD-PCR allowed the microorganism’s strains identification in beverages. Esters, aldehydes, superior alcohols and phenolic compounds were detected by gas chromatography. Three focus group (32 people; ≥ 18 years old) were organized to evaluate beverage’s acceptability. It was concluded that these beverages were stable from the chemical and microbiological point of view. Its global sensorial appreciation was above the average for all four beverages (> 60% - infusions; > 80% - juices).
Advisors/Committee Members: Prista, Catarina Geoffroy, Dias, Maria da Conceição Loureiro.
Subjects/Keywords: tea; juice; alcoholic fermentation; yeast; chá; sumo; fermentação alcoólica; levedura
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APA (6th Edition):
Oliveira, P. M. R. (2009). Utilização de leveduras na produção de novas bebidas fermentadas. (Thesis). Technical University of Lisbon. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/1093
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Oliveira, Pedro Miguel Rodrigues. “Utilização de leveduras na produção de novas bebidas fermentadas.” 2009. Thesis, Technical University of Lisbon. Accessed January 18, 2021.
http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/1093.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Oliveira, Pedro Miguel Rodrigues. “Utilização de leveduras na produção de novas bebidas fermentadas.” 2009. Web. 18 Jan 2021.
Vancouver:
Oliveira PMR. Utilização de leveduras na produção de novas bebidas fermentadas. [Internet] [Thesis]. Technical University of Lisbon; 2009. [cited 2021 Jan 18].
Available from: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/1093.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Oliveira PMR. Utilização de leveduras na produção de novas bebidas fermentadas. [Thesis]. Technical University of Lisbon; 2009. Available from: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/1093
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
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Open Access Theses and Dissertations