subject:( Oocytes)

University of Hawaii – Manoa

1. Tamura, Aileen Naomi. Mechanism of meiotic spindle disappearance and regeneration during oocyte vitrification.

Degree: 2016, University of Hawaii – Manoa

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M.S. University of Hawaii at Manoa 2011.

As assisted reproductive technologies (ART) become the standard of care for many infertile individuals, there has been a… (more)

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M.S. University of Hawaii at Manoa 2011.

As assisted reproductive technologies (ART) become the standard of care for many infertile individuals, there has been a growing demand for the technology to cryopreserve oocytes. A unique cryopreservation method, called vitrification, promises high rates of oocyte survival, fertilization and development. However, a potential problem with oocyte vitrification is the transient disappearance of the meiotic spindle during freezing. Normal development relies on the integrity of the meiotic spindle, and its malformations can lead to aberrant genetic segregation and birth defects. Our goal is to understand the mechanisms behind the meiotic spindle disappearance and reappearance during vitrification. This understanding is essential to develop safer and more effective vitrification procedures. To gain insight into the mechanisms, we examined the integrity of the spindle microtubules, and three centrosome components, NEDD1, pericentrin and γ-tubulin, in mouse oocytes at various steps during vitrification and thawing. The distribution of these components was assessed by immunostaining using specific antibodies. The Tyho-Galileo vitrification protocol was mainly used for this study, as it yielded the highest rate of oocyte survival in our hands. The spindle microtubules started to disappear gradually in the vitrification solutions, because of reduced temperatures and exposure to cryoprotectants, and became absent after exposure to liquid nitrogen. Regeneration of the spindle microtubules initiated during the thawing process, first in an excessive manner, generating a wide spindle and ectopic microtubule asters. However, the spindle was adjusted after the 37ºC incubation for normal size and shape, suggesting a dynamic nature of spindle regeneration. For centrosome components, partial staining of NEDD1 and γ-tubulin, but not pericentrin, was observed throughout the vitrification and thawing process. Thus, NEDD1 and γ-tubulin appear more resilient to the vitrification process than pericentrin, suggesting that they may serve as anchors in the centrosome to enable microtubule repolymerization during thawing. Our present study should lay a foundation for further studies to elucidate the mechanisms of spindle disappearance and regeneration during oocyte vitrification. Such insight would ultimately help improve the oocyte vitrification procedure to preserve fertility in women.

Subjects/Keywords: cryopreserve oocytes

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APA (6^th Edition):

Tamura, A. N. (2016). Mechanism of meiotic spindle disappearance and regeneration during oocyte vitrification. (Thesis). University of Hawaii – Manoa. Retrieved from http://hdl.handle.net/10125/101715

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tamura, Aileen Naomi. “Mechanism of meiotic spindle disappearance and regeneration during oocyte vitrification.” 2016. Thesis, University of Hawaii – Manoa. Accessed March 03, 2021. http://hdl.handle.net/10125/101715.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tamura, Aileen Naomi. “Mechanism of meiotic spindle disappearance and regeneration during oocyte vitrification.” 2016. Web. 03 Mar 2021.

Vancouver:

Tamura AN. Mechanism of meiotic spindle disappearance and regeneration during oocyte vitrification. [Internet] [Thesis]. University of Hawaii – Manoa; 2016. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10125/101715.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tamura AN. Mechanism of meiotic spindle disappearance and regeneration during oocyte vitrification. [Thesis]. University of Hawaii – Manoa; 2016. Available from: http://hdl.handle.net/10125/101715

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universiteit Utrecht

2. Vasse, E. Effect of age and media on the quality and developmental abilities of equine oocytes from slaughterhouse ovaries.

Degree: 2015, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/310724

► In vitro assisted reproductive techniques in horses have experienced considerable improvement in the last years. Although the results of in vitro fertilization are still very… (more)

Subjects/Keywords: equine; oocytes; age; DNA staining

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vasse, E. (2015). Effect of age and media on the quality and developmental abilities of equine oocytes from slaughterhouse ovaries. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/310724

Chicago Manual of Style (16^th Edition):

Vasse, E. “Effect of age and media on the quality and developmental abilities of equine oocytes from slaughterhouse ovaries.” 2015. Masters Thesis, Universiteit Utrecht. Accessed March 03, 2021. http://dspace.library.uu.nl:8080/handle/1874/310724.

MLA Handbook (7^th Edition):

Vasse, E. “Effect of age and media on the quality and developmental abilities of equine oocytes from slaughterhouse ovaries.” 2015. Web. 03 Mar 2021.

Vancouver:

Vasse E. Effect of age and media on the quality and developmental abilities of equine oocytes from slaughterhouse ovaries. [Internet] [Masters thesis]. Universiteit Utrecht; 2015. [cited 2021 Mar 03]. Available from: http://dspace.library.uu.nl:8080/handle/1874/310724.

Council of Science Editors:

Vasse E. Effect of age and media on the quality and developmental abilities of equine oocytes from slaughterhouse ovaries. [Masters Thesis]. Universiteit Utrecht; 2015. Available from: http://dspace.library.uu.nl:8080/handle/1874/310724

Universiteit Utrecht

3. Vasse, E. Effect of age and media on the quality and developmental abilities of equine oocytes from slaughterhouse ovaries.

Degree: 2015, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/320787

► In vitro assisted reproductive techniques in horses have experienced considerable improvement in the last years. Although the results of in vitro fertilization are still very… (more)

Subjects/Keywords: oocytes; equine; age; morphology; quality

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vasse, E. (2015). Effect of age and media on the quality and developmental abilities of equine oocytes from slaughterhouse ovaries. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/320787

Chicago Manual of Style (16^th Edition):

Vasse, E. “Effect of age and media on the quality and developmental abilities of equine oocytes from slaughterhouse ovaries.” 2015. Masters Thesis, Universiteit Utrecht. Accessed March 03, 2021. http://dspace.library.uu.nl:8080/handle/1874/320787.

MLA Handbook (7^th Edition):

Vasse, E. “Effect of age and media on the quality and developmental abilities of equine oocytes from slaughterhouse ovaries.” 2015. Web. 03 Mar 2021.

Vancouver:

Vasse E. Effect of age and media on the quality and developmental abilities of equine oocytes from slaughterhouse ovaries. [Internet] [Masters thesis]. Universiteit Utrecht; 2015. [cited 2021 Mar 03]. Available from: http://dspace.library.uu.nl:8080/handle/1874/320787.

Council of Science Editors:

Vasse E. Effect of age and media on the quality and developmental abilities of equine oocytes from slaughterhouse ovaries. [Masters Thesis]. Universiteit Utrecht; 2015. Available from: http://dspace.library.uu.nl:8080/handle/1874/320787

Victoria University of Wellington

4. Armstrong, Gina. Investigating the Effects of Oocytes on Proliferation Rate and Gene Expression of Mouse Ovarian Surface Epithelium-Derived Cancer Lines.

Degree: 2016, Victoria University of Wellington

URL: http://hdl.handle.net/10063/5655

► The origin of the most common form of ovarian cancer (OC), epithelial OC (EOC), remains a contentious issue. Due to disease heterogeneity, EOC is unlikely… (more)

▼ The origin of the most common form of ovarian cancer (OC), epithelial OC (EOC), remains a contentious issue. Due to disease heterogeneity, EOC is unlikely to originate from a single progenitor. This research explores an alternative hypothesis for the origin of EOC. During ovarian development, granulosa cells (GC) recruited from the ovarian surface epithelium (OSE) associate with oocytes. During follicular growth, oocyte-secreted growth factors (OSF) facilitate GC phenotype and function. Thus, if oocytes are lost prematurely from non-growing follicles, naïve GC remain. These cells, devoid of their germ cell regulator, may proliferate leading to neoplastic transformation and heterogeneous tumour phenotypes. This study aimed to elucidate the effects of OSF on (i) proliferation of, and (ii) candidate gene expression in, two mouse OSE-derived cancer cell lines, namely mOSE T2 (p53-/-/Akt/c-myc) and BR (p53-/-/Brca1-/-/Akt/c-myc). The OSF tested were oocyte-secreted media (OSM) containing rat OSF, as well recombinant (rec) porcine (p) BMP15 and pGDF9. Tritiated-thymidine uptake was used as a measure of cell proliferation and quantitative PCR was performed to measure gene expression levels of Cdh1 (epithelial marker), Foxl2 (granulosa cell marker), Dab2 and Muc16 (cancer markers). Exposure of mOSE T2 cells to OSM, but not rec pBMP15+pGDF9, resulted in decreased (P<0.02) proliferation rate but no change was observed in mOSE BR cells. Additionally, a decrease (P<0.02) in Muc16 mRNA levels was observed only in the T2 cell line incubated with OSM, but not rec pBMP15+pGDF9 and the BR cell line remained unaffected. Interestingly, Muc16 and Bmpr2 mRNA levels were lower overall in the mOSE BR, compared to the T2, cell line. In summary, both proliferation rate and expression levels of the tumourigenesis marker Muc16 were reduced in the mOSE T2 cell line after the addition of OSF. This supports the alternative hypothesis that proliferation of naïve OSE-derived GC is kept in check by OSF however, upon premature loss of oocytes or more specifically in the absence of OSF, these cells may proliferate and develop into EOC. Importantly, OSF were unable to suppress proliferation rate and Muc16 mRNA levels in cancer cells with a Brac1 mutation. Advisors/Committee Members: Pitman, Janet.

Subjects/Keywords: Oocytes; Surface epithelium; Ovarian

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Armstrong, G. (2016). Investigating the Effects of Oocytes on Proliferation Rate and Gene Expression of Mouse Ovarian Surface Epithelium-Derived Cancer Lines. (Masters Thesis). Victoria University of Wellington. Retrieved from http://hdl.handle.net/10063/5655

Chicago Manual of Style (16^th Edition):

Armstrong, Gina. “Investigating the Effects of Oocytes on Proliferation Rate and Gene Expression of Mouse Ovarian Surface Epithelium-Derived Cancer Lines.” 2016. Masters Thesis, Victoria University of Wellington. Accessed March 03, 2021. http://hdl.handle.net/10063/5655.

MLA Handbook (7^th Edition):

Armstrong, Gina. “Investigating the Effects of Oocytes on Proliferation Rate and Gene Expression of Mouse Ovarian Surface Epithelium-Derived Cancer Lines.” 2016. Web. 03 Mar 2021.

Vancouver:

Armstrong G. Investigating the Effects of Oocytes on Proliferation Rate and Gene Expression of Mouse Ovarian Surface Epithelium-Derived Cancer Lines. [Internet] [Masters thesis]. Victoria University of Wellington; 2016. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10063/5655.

Council of Science Editors:

Armstrong G. Investigating the Effects of Oocytes on Proliferation Rate and Gene Expression of Mouse Ovarian Surface Epithelium-Derived Cancer Lines. [Masters Thesis]. Victoria University of Wellington; 2016. Available from: http://hdl.handle.net/10063/5655

University of Edinburgh

5. Głuszek-Kustusz, Agnieszka Agata. Dissecting mechanisms of chromosomemicrotubule interaction in oocytes by new imaging tools.

Degree: PhD, 2014, University of Edinburgh

URL: http://hdl.handle.net/1842/18009

► Chromosome alignment and orientation within the spindle in mitosis and meiosis are determined by chromosome-microtubule interaction. Evidence suggests that within the acentrosomal spindle the mechanism… (more)

Subjects/Keywords: 571.8; kinetochore; spindle; oocytes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Głuszek-Kustusz, A. A. (2014). Dissecting mechanisms of chromosomemicrotubule interaction in oocytes by new imaging tools. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/18009

Chicago Manual of Style (16^th Edition):

Głuszek-Kustusz, Agnieszka Agata. “Dissecting mechanisms of chromosomemicrotubule interaction in oocytes by new imaging tools.” 2014. Doctoral Dissertation, University of Edinburgh. Accessed March 03, 2021. http://hdl.handle.net/1842/18009.

MLA Handbook (7^th Edition):

Głuszek-Kustusz, Agnieszka Agata. “Dissecting mechanisms of chromosomemicrotubule interaction in oocytes by new imaging tools.” 2014. Web. 03 Mar 2021.

Vancouver:

Głuszek-Kustusz AA. Dissecting mechanisms of chromosomemicrotubule interaction in oocytes by new imaging tools. [Internet] [Doctoral dissertation]. University of Edinburgh; 2014. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1842/18009.

Council of Science Editors:

Głuszek-Kustusz AA. Dissecting mechanisms of chromosomemicrotubule interaction in oocytes by new imaging tools. [Doctoral Dissertation]. University of Edinburgh; 2014. Available from: http://hdl.handle.net/1842/18009

University of Sydney

6. Lowe, Jenna Louise. Lipid metabolism during the in vitro production of porcine embryos .

Degree: 2014, University of Sydney

URL: http://hdl.handle.net/2123/13998

► Currently, the in vitro production (IVP) of porcine embryos suffers from low efficiency and reduced blastocyst quality. Poor outcomes from in vitro matured oocytes and… (more)

▼ Currently, the in vitro production (IVP) of porcine embryos suffers from low efficiency and reduced blastocyst quality. Poor outcomes from in vitro matured oocytes and in vitro fertilised embryos have limited the use of assisted reproductive technologies (ARTs) within commercial porcine herds, reducing the potential for global genetic improvement programs. It is believed that this reduced developmental competency compared to in vivo embryos is attributable to altered metabolism resulting from in vitro culture. Improper or incomplete metabolic support from the culture media leads to production of inferior embryos. Much of the prior research centres on metabolism of carbohydrates by oocytes and embryos, with the formulation of media based on this knowledge. However, oocytes and embryos also contain endogenous lipid substrates, and there is a lack of understanding as to how and when these stores are utilised. Lipids are a dense form of energy storage, and there is evidence of their metabolism by oocytes and embryos for energy generation. Porcine oocytes and embryos have higher intracellular lipid content than other domestic livestock species, and this makes them an excellent model for studying aspects of lipid metabolism in vitro. The aim of this study was to examine the impact of lipid metabolism on the acquisition of developmental competence during porcine IVP, and how this is affected by the presence or absence of exogenous carbohydrates. Stimulation or inhibition of the β-oxidation pathway was used to discern the importance of fatty acid oxidation to oocyte maturation and embryo development during in vitro maturation (IVM), in vitro fertilisation (IVF) and in vitro embryo culture (IVC). During IVM, it was identified that porcine oocytes are capable of using different substrates to compensate for deficiencies in others. While pyruvate and glucose are preferentially utilised to support maturation, upregulation of β-oxidation can compensate for a low glucose concentration and an absence of pyruvate to support nuclear maturation. Although there was no discernible decrease in lipid content associated with this, lipids provide such a dense energy reserve that any usage may have been beyond the limit of detection. Inhibition of β-oxidation in the absence of carbohydrates had a greater effect on nuclear maturation compared to inhibition in complete media. This indicates that lipid metabolism plays a minor role during oocyte maturation in the presence of carbohydrates and is likely to be more important when other substrates are deficient. Energy generation prior to fertilisation is an important factor in the developmental outcomes of subsequent embryos. Upregulation of β-oxidation for the duration of IVF increased cleavage rates, but doses above 6mM L-carnitine led to decreased blastocyst development. This effect may be attributable to the antioxidant activity of L-carnitine, with low levels of reactive oxygen species (ROS) being required at fertilisation for normal sperm function and sperm-oocyte interactions. Oocyte…

Subjects/Keywords: porcine; lipid metabolism; porcine oocytes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lowe, J. L. (2014). Lipid metabolism during the in vitro production of porcine embryos . (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/13998

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lowe, Jenna Louise. “Lipid metabolism during the in vitro production of porcine embryos .” 2014. Thesis, University of Sydney. Accessed March 03, 2021. http://hdl.handle.net/2123/13998.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lowe, Jenna Louise. “Lipid metabolism during the in vitro production of porcine embryos .” 2014. Web. 03 Mar 2021.

Vancouver:

Lowe JL. Lipid metabolism during the in vitro production of porcine embryos . [Internet] [Thesis]. University of Sydney; 2014. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/2123/13998.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lowe JL. Lipid metabolism during the in vitro production of porcine embryos . [Thesis]. University of Sydney; 2014. Available from: http://hdl.handle.net/2123/13998

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Alberta

7. Ahmad, Mohammad Sharif. Chromosomes and oocyte nuclei of the domestic fowl.

Degree: PhD, Department of Animal Science, 1969, University of Alberta

URL: https://era.library.ualberta.ca/files/3197xn88s

Subjects/Keywords: Chickens – Cytology.; Oocytes.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ahmad, M. S. (1969). Chromosomes and oocyte nuclei of the domestic fowl. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/3197xn88s

Chicago Manual of Style (16^th Edition):

Ahmad, Mohammad Sharif. “Chromosomes and oocyte nuclei of the domestic fowl.” 1969. Doctoral Dissertation, University of Alberta. Accessed March 03, 2021. https://era.library.ualberta.ca/files/3197xn88s.

MLA Handbook (7^th Edition):

Ahmad, Mohammad Sharif. “Chromosomes and oocyte nuclei of the domestic fowl.” 1969. Web. 03 Mar 2021.

Vancouver:

Ahmad MS. Chromosomes and oocyte nuclei of the domestic fowl. [Internet] [Doctoral dissertation]. University of Alberta; 1969. [cited 2021 Mar 03]. Available from: https://era.library.ualberta.ca/files/3197xn88s.

Council of Science Editors:

Ahmad MS. Chromosomes and oocyte nuclei of the domestic fowl. [Doctoral Dissertation]. University of Alberta; 1969. Available from: https://era.library.ualberta.ca/files/3197xn88s

Universidade Federal de Viçosa

8. Giancarlo Magalhães dos Santos. Diferentes tempos de exposição e meios para o transporte na taxa de maturação "in vitro" de ovócitos bovinos.

Degree: 2008, Universidade Federal de Viçosa

URL: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=1645

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O objetivo do presente trabalho foi avaliar e comparar a influência dos diferentes tempos de exposição e meios no transporte de ovócitos imaturos bovinos sobre… (more)

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O objetivo do presente trabalho foi avaliar e comparar a influência dos diferentes tempos de exposição e meios no transporte de ovócitos imaturos bovinos sobre a taxa de maturação in vitro. Foram utilizados 799 ovócitos imaturos, distribuídos em dez tratamentos, sendo um controle. Foram testados três meios de transporte: Talp-Hepes, segundo BAVISTER et al. (1983); Talp-Hepes modificado, acrescido de 10 g/mL de FSH e 0,0454 mM de Piruvato de Sódio e TCM modificado sem NaHCO3, acrescido de 5,665 mM de Hepes, 0,999 mM de NaCl, 0,0412 mM de Lactato de Cálcio, 0,0454 mM de Piruvato de Sódio, 10.000 UI de Penicilina G sódica, 0,005g% de Estreptomicina, 0,4g% de BSA e 10 g/mL de FSH. Em cada um dos meios utilizados, os ovócitos foram mantidos em placa aquecida a 38C por um período de duas, quatro e oito horas. Após o término do tempo de exposição, os ovócitos foram submetidos a cultivo in vitro em meio TCM 199 acrescido de 10% de SVE e 10 g/mL de FSH, em estufa de CO2, por 24 horas, para posterior avaliação da taxa de maturação nuclear. No tratamento controle, após a decantação e seleção dos ovócitos, eles foram imediatamente submetidos ao procedimento de cultivo in vitro. Observou-se que nos tratamentos com Talp-Hepes em tempos de duas, quatro e oito horas foram obtidas taxas de maturação nuclear de 84,7; 81,2 e 81,3%, respectivamente. No meio Talp-Hepes modificado foram registradas taxas de 72,2; 82,0 e 82,2%, e no meio TCM modificado, taxas de 80,0; 78,6 e 80,0%, respectivamente, para os tempos duas, quatro e oito horas. No tratamento controle, observou-se 80,1% de metáfase II. Conclui-se que os meios utilizados para o transporte dos ovócitos imaturos e o tempo decorrido da coleta até incubação para a maturação in vitro não influenciaram (P>0,05) a taxa de maturação nuclear. Deste modo, estes meios se mostram uma possível alternativa para o transporte dos ovócitos bovinos destinados à produção in vitro de embriões.

The objective of this work was to assess and compare the influence of different expositon time and transportation media on in vitro maturation of immature bovine ocytes. We used 799 immature oocytes, distributed in ten treatments, one of them, the control. Three transportation media were tested: Talp-Hepes, according to BAVISTER et al. (1983); modified Talp-Hepes, with 10 g/mL of FSH and 0,0454 mM of Sodium Piruvate and modified TCM without NaHCO3, with 5,665 mM of Hepes, 0,999 mM of NaCl, 0,0412 mM of Calcium Lactate, 0,0454 mM of Sodium Piruvate, 10.000 UI of Sodic Penicillin G, 0,005g% of Streptomycin, 0,4g% of BSA and 10 g/mL of FSH. In each of the used media, the oocyte were kept on a plate and heated at 38 °C for two, four and eight hours. After this time, the oocytes were submitted to in vitro cultivation in TCM 199 media with 10% of SVE and 10 g/mL of FSH, in CO2 oven, for 24 hours, for later nuclear maturation rate evaluation. In the control, after the oocyte decanting and selection, they were immediately submitted to in vitro cultivation procedure. We observed that in the Talp-Hepes treatment with…

Advisors/Committee Members: Carlos Antônio de Carvalho Fernandes, Tarcízio Antônio Rego de Paula, Luiz Sergio de Almeida Camargo, João Henrique Moreira Viana, José Domingos Guimarães, Orlando Marcelo Vendramini, Eduardo Paulino da Costa.

Subjects/Keywords: REPRODUCAO ANIMAL; Bovinos; Ovócitos; FIV; Bovine; Oocytes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Santos, G. M. d. (2008). Diferentes tempos de exposição e meios para o transporte na taxa de maturação "in vitro" de ovócitos bovinos. (Thesis). Universidade Federal de Viçosa. Retrieved from http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=1645

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Santos, Giancarlo Magalhães dos. “Diferentes tempos de exposição e meios para o transporte na taxa de maturação "in vitro" de ovócitos bovinos.” 2008. Thesis, Universidade Federal de Viçosa. Accessed March 03, 2021. http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=1645.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Santos, Giancarlo Magalhães dos. “Diferentes tempos de exposição e meios para o transporte na taxa de maturação "in vitro" de ovócitos bovinos.” 2008. Web. 03 Mar 2021.

Vancouver:

Santos GMd. Diferentes tempos de exposição e meios para o transporte na taxa de maturação "in vitro" de ovócitos bovinos. [Internet] [Thesis]. Universidade Federal de Viçosa; 2008. [cited 2021 Mar 03]. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=1645.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Santos GMd. Diferentes tempos de exposição e meios para o transporte na taxa de maturação "in vitro" de ovócitos bovinos. [Thesis]. Universidade Federal de Viçosa; 2008. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=1645

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Edinburgh

9. Nikalayevich, Elvira. Meiotic spindle organization and chromosome condensation in Drosophila oocytes.

Degree: PhD, 2014, University of Edinburgh

URL: http://hdl.handle.net/1842/17908

► Errors in chromosome segregation during the first division of female meiosis are very common in humans and result in aneuploidy leading to reproduction problems. Chromosome… (more)

▼ Errors in chromosome segregation during the first division of female meiosis are very common in humans and result in aneuploidy leading to reproduction problems. Chromosome segregation depends on the formation and function of the meiotic spindle as well as the structure of chromosomes, which need to condense to be able to orient and segregate properly. It is important to understand the mechanisms underlying the female meiotic spindle function and chromosome condensation to gain insight into female fertility problems. The female meiotic spindle assembles without centrosomes, so the mechanisms ensuring microtubule nucleation, spindle assembly and establishment of bipolarity act differently from those of mitosis or male meiosis. I identified a set of genes that are required for microtubule nucleation, spindle maintenance and centromere orientation in Drosophila female meiosis. This was accomplished by mapping previously uncharacterized Drosophila mutants and depleting already known genes by RNAi. I discovered that several proteins have a different role in female meiosis as compared to mitosis, which provides insight into the major differences between these systems. Little is known about the molecular mechanisms of chromosome condensation. The roles of only a few factors, such as condensin complexes, have been studied previously, and the evidence suggests that there are more molecular players required for chromosome condensation. To discover molecular mechanisms critical to this process, I depleted various chromosomal proteins by RNAi and screened for abnormalities of metaphase chromosome morphology in Drosophila oocytes by immunostaining and live imaging. I found that the conserved kinase NHK-1 plays a role in chromosome condensation in female meiosis. BAF is a critical NHK-1 substrate in this process and its phosphorylation is required for detachment of the chromosomes from the nuclear envelope to allow proper condensation. Also, I discovered that the nucleosome remodelling complex NuRD is crucial for chromosome condensation, especially for the chromosome arms. As a result of my PhD project I identified multiple factors required for meiotic spindle function. I also discovered two novel pathways of chromosome condensation that require the NuRD complex and NHK-1 activity.

Subjects/Keywords: 572.8; condensation; spindle; Drosophila; meiosis; oocytes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nikalayevich, E. (2014). Meiotic spindle organization and chromosome condensation in Drosophila oocytes. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/17908

Chicago Manual of Style (16^th Edition):

Nikalayevich, Elvira. “Meiotic spindle organization and chromosome condensation in Drosophila oocytes.” 2014. Doctoral Dissertation, University of Edinburgh. Accessed March 03, 2021. http://hdl.handle.net/1842/17908.

MLA Handbook (7^th Edition):

Nikalayevich, Elvira. “Meiotic spindle organization and chromosome condensation in Drosophila oocytes.” 2014. Web. 03 Mar 2021.

Vancouver:

Nikalayevich E. Meiotic spindle organization and chromosome condensation in Drosophila oocytes. [Internet] [Doctoral dissertation]. University of Edinburgh; 2014. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1842/17908.

Council of Science Editors:

Nikalayevich E. Meiotic spindle organization and chromosome condensation in Drosophila oocytes. [Doctoral Dissertation]. University of Edinburgh; 2014. Available from: http://hdl.handle.net/1842/17908

10. Li, Fang. Age-Related Hamster Mitochondrial Changes and Oocyte Changes Following Autologous Platelet Mitochondrial Microinjection.

Degree: PhD, 2011, Old Dominion University

URL: 9781124990705 ; https://digitalcommons.odu.edu/biomedicalsciences_etds/53

► This study's objective was to verify age-related mitochondrial changes in oocytes from old hamsters compared with those from young hamsters. We used autologous platelet… (more)

▼ This study's objective was to verify age-related mitochondrial changes in oocytes from old hamsters compared with those from young hamsters. We used autologous platelet mitochondrial microinjection to improve the mitochondrial quality and quantity of aged oocytes to improve their pregnancy potential. Metaphase II oocytes were collected from super-ovulated female hamsters and prepared for biochemical and morphological analysis. Adenosine triphosphate (ATP) levels, mitochondrial DNA (mtDNA) number, reactive oxygen species (ROS) level and mitochondrial membrane potential (MMP) were determined in individual oocytes from young and old hamsters. Transmission electron microscopy (TEM) allowed evaluation of oocyte ultra-structure. In oocytes from old hamsters, ATP levels and mtDNA number were reduced an average of 35.4% and 51.8% respectively compared to young oocytes. Lower MMP and higher ROS levels were observed in oocytes from old hamsters compared with those from young hamsters. TEM analysis supported low mitochondrial quantity and increased mitochondrial electron density in old oocytes, along with collapsed cytoplasmic lamellae. When comparing platelet mitochondria between young and old hamsters, no significant difference was found in ATP level per mitochondrion or ultrastructure. After autologous platelet mitochondrial microinjection, ATP production increased significantly in the microinjected group, however mtDNA number and embryo development to blastocyst stage were not improved. MitoTracker Green FM (MGF) was used to help quantify mitochondria, but the results conflicted with mtDNA and TEM data, which suggests that MGF, as a MMP dependent reagent, is not an ideal method to reflect mitochondrial number. Our data suggest that (1) age-related morphological and functional mitochondrial changes occur in oocytes from old hamsters compared with young hamsters; (2) platelets can be used as a source for mitochondrial transfer; and (3) autologous platelet mitochondrial microinjection improves mitochondrial ATP production in old oocytes. The fact that no change in blastocyst development after microinjection was observed does not rule out that there might not be improvement of post-implantation embryo development. Therefore, further study will focus on effect of autologous platelet mitochondrial microinjection on post-implantation embryo development. Advisors/Committee Members: R. James Swanson, Frank J. Castora, Christopher Osgood, David T. Gauthier.

Subjects/Keywords: Mitochondrial changes; Oocytes; Platelets; Cell Biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Li, F. (2011). Age-Related Hamster Mitochondrial Changes and Oocyte Changes Following Autologous Platelet Mitochondrial Microinjection. (Doctoral Dissertation). Old Dominion University. Retrieved from 9781124990705 ; https://digitalcommons.odu.edu/biomedicalsciences_etds/53

Chicago Manual of Style (16^th Edition):

Li, Fang. “Age-Related Hamster Mitochondrial Changes and Oocyte Changes Following Autologous Platelet Mitochondrial Microinjection.” 2011. Doctoral Dissertation, Old Dominion University. Accessed March 03, 2021. 9781124990705 ; https://digitalcommons.odu.edu/biomedicalsciences_etds/53.

MLA Handbook (7^th Edition):

Li, Fang. “Age-Related Hamster Mitochondrial Changes and Oocyte Changes Following Autologous Platelet Mitochondrial Microinjection.” 2011. Web. 03 Mar 2021.

Vancouver:

Li F. Age-Related Hamster Mitochondrial Changes and Oocyte Changes Following Autologous Platelet Mitochondrial Microinjection. [Internet] [Doctoral dissertation]. Old Dominion University; 2011. [cited 2021 Mar 03]. Available from: 9781124990705 ; https://digitalcommons.odu.edu/biomedicalsciences_etds/53.

Council of Science Editors:

Li F. Age-Related Hamster Mitochondrial Changes and Oocyte Changes Following Autologous Platelet Mitochondrial Microinjection. [Doctoral Dissertation]. Old Dominion University; 2011. Available from: 9781124990705 ; https://digitalcommons.odu.edu/biomedicalsciences_etds/53

11. Yu, Changwei. Analysis of the composition and the function of oocyte-specific TBP2-containing transcription machinery during mouse oogenesis : Analyse de la composition et de la fonction de la machinerie basale de transcription associée à TBP2 et spécifique des ovocytes au cours de l’ovogenèse murine.

Degree: Docteur es, Aspects moléculaires et cellulaires de la biologie, 2018, Université de Strasbourg

URL: http://www.theses.fr/2018STRAJ127

►

La synthèse d’ARN au cours de la différenciation des ovocytes est essentielle à la fécondation et à l'initiation du développement précoce. La nature de la… (more)

▼

La synthèse d’ARN au cours de la différenciation des ovocytes est essentielle à la fécondation et à l'initiation du développement précoce. La nature de la machinerie basale de transcription pendant la croissance ovocytaire n'est pas connue mais la protéine TBP est remplacée par une protéine semblable spécifique des vertébrés, TBP2. Pour comprendre le rôle de TBP2 dans l'initiation de la transcription, nous avons effectué un RNA-seq à partir d'ovocytes contrôles et Tbp2-/- et montré que l'expression des gènes les plus transcrits ainsi celle des éléments rétroviraux endogènes de type MaLR est diminuée. Par immunoprécipitation couplée à la spectrométrie de masse à partir d'ovaires, nous avons montré que TBP2 ne forme pas un complexe TFIID, mais est associé à TFIIA dans les ovocytes. Globalement nos données montrent qu’une machinerie d'initiation de la transcription spécifique différente du complexe canonique TFIID contrôle la transcription dans les ovocytes de souris.

Mammalian oocytes go through consecutive differentiation process, during which the synthesis and accumulation of RNAs are essential for oocyte growth, maturation, fertilization and early embryogenesis. Little is known about the nature and function of the oocyte Pol II transcription machinery. During oocyte growth TBP is replaced by a vertebrate specific paralog, TBP2, and Tbp2-/- females are sterile. To understand whether and how TBP2 is controlling transcription initiation during oogenesis, we carried out RNA-seq analyses from wild-type and Tbp2-/- oocytes from primary and secondary follicles. These analyses show a main decrease in the expression of the most abundant genes as well as specific down-regulation of the expression of the MaLR-type endogenous retroviral elements. To identify the nature of the complex associated with TBP2 in the oocytes, we carried out immunoprecipitation followed by mass spectrometry. We demonstrate that, in the oocytes, TBP2 associates with TFIIA, but does not assemble into a TFIID-type complex. Altogether, our data show that a specific TBP2-TFIIA-containing transcription machinery, different from canonical TFIID, drives transcription in mouse oocytes.

Advisors/Committee Members: Tora, Laszlo (thesis director), Vincent, Stéphane (thesis director).

Subjects/Keywords: TFIID; TBP2; RNA polymerase II; TFIIA; MaLR; Oocytes; TFIID; TBP2; RNA polymerase II; TFIIA; MaLR; Oocytes; 571.86

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yu, C. (2018). Analysis of the composition and the function of oocyte-specific TBP2-containing transcription machinery during mouse oogenesis : Analyse de la composition et de la fonction de la machinerie basale de transcription associée à TBP2 et spécifique des ovocytes au cours de l’ovogenèse murine. (Doctoral Dissertation). Université de Strasbourg. Retrieved from http://www.theses.fr/2018STRAJ127

Chicago Manual of Style (16^th Edition):

Yu, Changwei. “Analysis of the composition and the function of oocyte-specific TBP2-containing transcription machinery during mouse oogenesis : Analyse de la composition et de la fonction de la machinerie basale de transcription associée à TBP2 et spécifique des ovocytes au cours de l’ovogenèse murine.” 2018. Doctoral Dissertation, Université de Strasbourg. Accessed March 03, 2021. http://www.theses.fr/2018STRAJ127.

MLA Handbook (7^th Edition):

Yu, Changwei. “Analysis of the composition and the function of oocyte-specific TBP2-containing transcription machinery during mouse oogenesis : Analyse de la composition et de la fonction de la machinerie basale de transcription associée à TBP2 et spécifique des ovocytes au cours de l’ovogenèse murine.” 2018. Web. 03 Mar 2021.

Vancouver:

Yu C. Analysis of the composition and the function of oocyte-specific TBP2-containing transcription machinery during mouse oogenesis : Analyse de la composition et de la fonction de la machinerie basale de transcription associée à TBP2 et spécifique des ovocytes au cours de l’ovogenèse murine. [Internet] [Doctoral dissertation]. Université de Strasbourg; 2018. [cited 2021 Mar 03]. Available from: http://www.theses.fr/2018STRAJ127.

Council of Science Editors:

Yu C. Analysis of the composition and the function of oocyte-specific TBP2-containing transcription machinery during mouse oogenesis : Analyse de la composition et de la fonction de la machinerie basale de transcription associée à TBP2 et spécifique des ovocytes au cours de l’ovogenèse murine. [Doctoral Dissertation]. Université de Strasbourg; 2018. Available from: http://www.theses.fr/2018STRAJ127

University of Alberta

12. Behrouzi, Amir. Effect of porcine luteinizing hormone on intrafollicular milieu and gene expression in granulosa cells and oocytes in dairy cows.

Degree: MS, Department of Agricultural, Food, and Nutritional Science, 2014, University of Alberta

URL: https://era.library.ualberta.ca/files/6w924c30w

► In previous research, the use of porcine luteinizing hormone (pLH) in lieu of gonadotropin-releasing hormone (GnRH) for synchronizing ovulation in a fixed-timed artificial insemination protocol… (more)

Subjects/Keywords: Follicle; Granulosa cells; Dairy cows; Pregnancy rate; Luteinizing hormone; Oocytes

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APA (6^th Edition):

Behrouzi, A. (2014). Effect of porcine luteinizing hormone on intrafollicular milieu and gene expression in granulosa cells and oocytes in dairy cows. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/6w924c30w

Chicago Manual of Style (16^th Edition):

Behrouzi, Amir. “Effect of porcine luteinizing hormone on intrafollicular milieu and gene expression in granulosa cells and oocytes in dairy cows.” 2014. Masters Thesis, University of Alberta. Accessed March 03, 2021. https://era.library.ualberta.ca/files/6w924c30w.

MLA Handbook (7^th Edition):

Behrouzi, Amir. “Effect of porcine luteinizing hormone on intrafollicular milieu and gene expression in granulosa cells and oocytes in dairy cows.” 2014. Web. 03 Mar 2021.

Vancouver:

Behrouzi A. Effect of porcine luteinizing hormone on intrafollicular milieu and gene expression in granulosa cells and oocytes in dairy cows. [Internet] [Masters thesis]. University of Alberta; 2014. [cited 2021 Mar 03]. Available from: https://era.library.ualberta.ca/files/6w924c30w.

Council of Science Editors:

Behrouzi A. Effect of porcine luteinizing hormone on intrafollicular milieu and gene expression in granulosa cells and oocytes in dairy cows. [Masters Thesis]. University of Alberta; 2014. Available from: https://era.library.ualberta.ca/files/6w924c30w

13. Chroni, Dafni. Ηλεκτροφυσιολογική και βιοχημική μελέτη υποδοχέων σχετιζομένων με ασθένειες του κεντρικού νευρικού συστήματος.

Degree: 2018, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/43453

► Neuronal nicotinic acetylcholine receptors (nAChRs) are located mainly in the Central Nervous System (CNS) where they regulate the release of neurotransmitters and mediate fast neurotransmission.… (more)

▼ Neuronal nicotinic acetylcholine receptors (nAChRs) are located mainly in the Central Nervous System (CNS) where they regulate the release of neurotransmitters and mediate fast neurotransmission. Neurotransmitters transfer the information from a neuron to an adjacent neuron or muscle cell. Some examples of neurotransmitters are acetylcholine (ACh), serotonin, γ-aminobutyric acid (GABA) and glycine. nAChRs belong to the ‘Cys-loop’ super-family of pentameric ligand-gated ion channels (pLGIC), since this type of receptors possess a characteristic loop formed by 13 highly conserved amino acids between two cysteine (Cys) residues bridged by a disulfide bond, at the N-terminal extracellular domain (ECD). Moreover the Cys-loop superfamily includes the γ-aminobutyric acid (GABAA and GABAC), glycine and serotonin (5-HT3) receptors. They consist of the combination of 9 α (α2-α10) and 3 β (β2-β4) subunits and they are distributed ubiquitously in the brain. The neuronal nAChRs exist either as homopentameric or as heteropentameric molecules. The α7 and α9 subunits are the only known human nAChR subunits that can form homopentamers. They are implicated in various neurological diseases, such as Alzheimer and Parkinson diseases, epilepsy, attention deficit hyperactivity disorder, depression, neuropathic pain and substance addiction. Thus, drug development for these receptors is a priority. These neurotransmitters bind to the ECD of the corresponding receptors, thus, it is very important to know the 3D structure of this region. In general, the ECD of neuronal nAChRs, consists of an N-terminal α-helix and a hydrophobic core of 10 β-strands (β1-β10). Some of the characteristic loops, linking these β-strands, contribute to the formation of the ligand- binding site. Loops A, B, and C contribute to the formation of the primary (+) binding site, whereas the loops D, E and F conferred from the adjacent subunit form the complementary (-) side of the ligand binding site.The principal binding sides of the neuronal nAChRs are very homologous among the various subunits, whereas there is little conservation in the complementary sides, which are those that confer selectivity to various drugs. Thus, in order to design highly specific and effective drugs towards a specific nAChR subtype and avoid side-effects, its in-depth structural and functional information is highly needed. Towards this aim, members of our laboratory have recently acquired important structural crystallographic data for the ECDs of two subunits of human nAChRs: the α9 and α2. In the nature, the α9 subunit forms either homopentamers or heteropentamers with α10, whereas α2 is known to form heteropentamers with the β2 subunit. It is worth mentioning that α2 presents high homology with the α4 subunit (78%), which is the most commonly found nAChR subunit in the human brain. In the above studies, the crystal structures of the ECDs of the human α9 and α2 subunits were presented. The structure of α9-ECD was determined as a monomer in its free-state and in its complexes with the…

Subjects/Keywords: Ωοκύτταρα; Νικοτινικός υποδοχέας ακετυλοχολίνης; Oocytes; Nicotinic acetylcholine receptor

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chroni, D. (2018). Ηλεκτροφυσιολογική και βιοχημική μελέτη υποδοχέων σχετιζομένων με ασθένειες του κεντρικού νευρικού συστήματος. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/43453

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chroni, Dafni. “Ηλεκτροφυσιολογική και βιοχημική μελέτη υποδοχέων σχετιζομένων με ασθένειες του κεντρικού νευρικού συστήματος.” 2018. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed March 03, 2021. http://hdl.handle.net/10442/hedi/43453.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chroni, Dafni. “Ηλεκτροφυσιολογική και βιοχημική μελέτη υποδοχέων σχετιζομένων με ασθένειες του κεντρικού νευρικού συστήματος.” 2018. Web. 03 Mar 2021.

Vancouver:

Chroni D. Ηλεκτροφυσιολογική και βιοχημική μελέτη υποδοχέων σχετιζομένων με ασθένειες του κεντρικού νευρικού συστήματος. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2018. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10442/hedi/43453.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chroni D. Ηλεκτροφυσιολογική και βιοχημική μελέτη υποδοχέων σχετιζομένων με ασθένειες του κεντρικού νευρικού συστήματος. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2018. Available from: http://hdl.handle.net/10442/hedi/43453

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Jawaharlal Nehru University

14. Pathak, Narendra H. Molecular characterisation of gen; Molecular characterisation of gene(s) expressped specifically in snake oocytes.

Degree: Molecular biology, 1997, Jawaharlal Nehru University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/17055

None

Bibliography p. 72-95

Advisors/Committee Members: Singh, Lalji.

Subjects/Keywords: Molecular biology; Snake oocytes

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APA (6^th Edition):

Pathak, N. H. (1997). Molecular characterisation of gen; Molecular characterisation of gene(s) expressped specifically in snake oocytes. (Thesis). Jawaharlal Nehru University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/17055

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pathak, Narendra H. “Molecular characterisation of gen; Molecular characterisation of gene(s) expressped specifically in snake oocytes.” 1997. Thesis, Jawaharlal Nehru University. Accessed March 03, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/17055.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pathak, Narendra H. “Molecular characterisation of gen; Molecular characterisation of gene(s) expressped specifically in snake oocytes.” 1997. Web. 03 Mar 2021.

Vancouver:

Pathak NH. Molecular characterisation of gen; Molecular characterisation of gene(s) expressped specifically in snake oocytes. [Internet] [Thesis]. Jawaharlal Nehru University; 1997. [cited 2021 Mar 03]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/17055.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pathak NH. Molecular characterisation of gen; Molecular characterisation of gene(s) expressped specifically in snake oocytes. [Thesis]. Jawaharlal Nehru University; 1997. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/17055

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

15. Saxena, R C. Cytological and histo chemical studies on the cortical zone of oocytes; -.

Degree: Zoology, 1979, INFLIBNET

URL: http://shodhganga.inflibnet.ac.in/handle/10603/38677

None

Bibliography p.165-185

Advisors/Committee Members: Sahai, Y M.

Subjects/Keywords: Cortical; Cytological; Histo Chemical; Oocytes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Saxena, R. C. (1979). Cytological and histo chemical studies on the cortical zone of oocytes; -. (Thesis). INFLIBNET. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/38677

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Saxena, R C. “Cytological and histo chemical studies on the cortical zone of oocytes; -.” 1979. Thesis, INFLIBNET. Accessed March 03, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/38677.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Saxena, R C. “Cytological and histo chemical studies on the cortical zone of oocytes; -.” 1979. Web. 03 Mar 2021.

Vancouver:

Saxena RC. Cytological and histo chemical studies on the cortical zone of oocytes; -. [Internet] [Thesis]. INFLIBNET; 1979. [cited 2021 Mar 03]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/38677.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Saxena RC. Cytological and histo chemical studies on the cortical zone of oocytes; -. [Thesis]. INFLIBNET; 1979. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/38677

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

16. Kharya, Vandana. Role of follicular epithelium in the resorption of oocytes in some insects; -.

Degree: Zoology, 2002, INFLIBNET

URL: http://shodhganga.inflibnet.ac.in/handle/10603/38138

None

Bibliography p. 142-157

Advisors/Committee Members: Bhide, Mangla.

Subjects/Keywords: Epithelium; Follicular; Insect; Oocytes; Resorption

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kharya, V. (2002). Role of follicular epithelium in the resorption of oocytes in some insects; -. (Thesis). INFLIBNET. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/38138

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kharya, Vandana. “Role of follicular epithelium in the resorption of oocytes in some insects; -.” 2002. Thesis, INFLIBNET. Accessed March 03, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/38138.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kharya, Vandana. “Role of follicular epithelium in the resorption of oocytes in some insects; -.” 2002. Web. 03 Mar 2021.

Vancouver:

Kharya V. Role of follicular epithelium in the resorption of oocytes in some insects; -. [Internet] [Thesis]. INFLIBNET; 2002. [cited 2021 Mar 03]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/38138.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kharya V. Role of follicular epithelium in the resorption of oocytes in some insects; -. [Thesis]. INFLIBNET; 2002. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/38138

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Federal de Viçosa

17. Giancarlo Magalhães dos Santos. Vitrificação de ovócitos imaturos de bovinos.

Degree: 2011, Universidade Federal de Viçosa

URL: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=3801

► Objetivou-se avaliar o efeito de soluções vitrificantes e da vitrificação em ovócitos imaturos bovino. Os ovócitos foram obtidos a partir de ovários coletados de fêmeas… (more)

▼ Objetivou-se avaliar o efeito de soluções vitrificantes e da vitrificação em ovócitos imaturos bovino. Os ovócitos foram obtidos a partir de ovários coletados de fêmeas bovinas logo após o abate, selecionados morfologicamente e distribuídos de acordo com os tratamentos. O procedimento experimental foi dividido em duas fases. Na primeira fase foi testada a toxicidade de soluções de vitrificação. Na segunda fase, foi testado o efeito da vitrificação. Na primeira fase, os ovócitos foram submetidos à maturação in vitro após manutenção em diferentes soluções de vitrificação (61 tratamentos). Foram testadas soluções de equilíbrio (SE) de 3, 8, 16, 20, 32 e 40% de Etilenoglicol (EG) em tempos (TE) de 1, 2, 4, 5, 6, 8 e 10 minutos e posteriormente mantidos por 1 minuto em solução de vitrificação (SV) contendo 40% de EG e 1,0 mol.L-1 de sacarose. O mesmo procedimento foi realizado, porém, utilizando solução de vitrificação contendo 25% de EG, 25% de DMSO e 1,0 mol.L-1 de sacarose. Após estes procedimentos, os ovócitos foram reidratados e submetidos ao cultivo in vitro por 24 horas. Observou-se maturação nuclear em grande parte dos tratamentos com exceção das combinações de SE com 32 e 40% de EG nos diferentes TE. Da mesma forma, os ovócitos expostos à SV de 25% de EG + 25% de DMSO + 1 mol.L-1 de sacarose não possibilitaram a maturação. Os melhores resultados foram as soluções de SE contendo 3%, 16%, expostas por cinco minutos e 20% de EG, expostas por 10 minutos (76,7, 57,1 e 66,7% de ovócitos em metáfase II, respectivamente). Todos estes tratamentos foram expostos ao final do equilíbrio à SV, contendo 40% de EG + 1,0 mol.L-1 de sacarose. Na segunda etapa, ovócitos foram submetidos aos procedimentos conforme descrito na primeira fase. Foram testadas SE com 3% de EG (por 1, 5, 6, 8, e 10 minutos), 8% de EG (por 1, 5 e 10 minutos), 16 e 20% de EG (por 5 e 8 minutos). Posteriormente, os ovócitos foram mantidos por 1 minuto em SV contendo 40% de EG e 1,0 mol.L-1 de sacarose. Posteriormente, os ovócitos foram envasados em palhetas de 0,25 mL, vitrificados (imersos em nitrogênio líquido) e posteriormente desvitrificados, rehidratados e submetidos à maturação in vitro por 24 horas. Observou-se que as combinações SE com 3% de EG, com TE de 1, 5, 6, 8 e 10 minutos proporcionaram taxas de metáfase II de 10,4; 19,3; 10,0; 7,6 e 14,3% respectivamente. As combinações SE com 8% de EG, com TE de 1, 5 e 10, e SE com 16% de EG, com TE de 5 minutos e SE de 20% de EG, com TE de 8 minutos proporcionaram taxas de metáfase II de 12,9; 6,9; 2,4; 23,1 e 2,0% respectivamente, sendo que todas diferem (P<0,05) ao grupo testemunha (73,7%). Foram processados ovócitos para a avaliação da ultraestrutura e expressão gênica. A ultraestrutura indicou que ovócitos de todos os tratamentos testados (exceto o grupo testemunha) apresentavam organelas degeneradas. Adicionalmente, não foi observada a distribuição citoplasmática típica de mitocôndrias de um ovócito maduro. Já na expressão gênica observou-se que ovócitos expostos a solução de equilíbrio de 16% de EG… Advisors/Committee Members: Carlos Antônio de Carvalho Fernandes, Tarcízio Antônio Rego de Paula, Luiz Sergio de Almeida Camargo, João Henrique Moreira Viana, Claudio José Borela Espeschit, Laércio dos Anjos Benjamin, Giuliano Moraes Figueiró, Eduardo Paulino da Costa.

Subjects/Keywords: Vitrificação; Oocytes; Bovine; Vitrification; REPRODUCAO ANIMAL; Ovócitos; Bovinos

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Santos, G. M. d. (2011). Vitrificação de ovócitos imaturos de bovinos. (Thesis). Universidade Federal de Viçosa. Retrieved from http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=3801

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Santos, Giancarlo Magalhães dos. “Vitrificação de ovócitos imaturos de bovinos.” 2011. Thesis, Universidade Federal de Viçosa. Accessed March 03, 2021. http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=3801.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Santos, Giancarlo Magalhães dos. “Vitrificação de ovócitos imaturos de bovinos.” 2011. Web. 03 Mar 2021.

Vancouver:

Santos GMd. Vitrificação de ovócitos imaturos de bovinos. [Internet] [Thesis]. Universidade Federal de Viçosa; 2011. [cited 2021 Mar 03]. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=3801.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Santos GMd. Vitrificação de ovócitos imaturos de bovinos. [Thesis]. Universidade Federal de Viçosa; 2011. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=3801

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Federal de Viçosa

18. Gustavo Bruno Mota. Desenvolvimento e expressão gênica em oócitos bovinos imaturos selecionados por Azul Cresil Brilhante.

Degree: 2008, Universidade Federal de Viçosa

URL: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=1345

►

The in vitro embryo production (IVP) efficiency has been limited by oocyte developmental competence and in vitro culture conditions. The use of Brilliant Cresyl Blue… (more)

▼

The in vitro embryo production (IVP) efficiency has been limited by oocyte developmental competence and in vitro culture conditions. The use of Brilliant Cresyl Blue (BCB) dye has been described as an alternative method for selection of better quality oocytes. The aim of this work was to evaluate the selection of immature oocytes by BCB dye and the expression of transcripts involved in the transition from genome-maternal. In the first step, a total of 998 immature oocytes was distributed into: G1- Control: oocytes sent directly to in vitro maturation, not exposed to the dye; G2- Control incubation (mPBS): oocytes exposed to mPBS solution without BCB for 1h; G3- BCB+: oocytes exposed to mPBS added of BCB and positively stained; G4- BCB-: oocytes exposed to mPBS with BCB and colorless. Oocytes of each treatment were matured, fertilized and presumptive zygotes were cultured in vitro for eight days. The rates of cleavage were obtained at 48h after the beginning of the culture and the rates of blastocysts at eight days post fertilization. The cleavage rates between G1 and G3 were similar, but higher than G2 and G4, the ones which similar. The oocytes in G3 showed blastocyst rate similar to the G1 and G2, but higher than G4. In another step, total RNA was extracted from three pools of 12 immature oocytes obtained from each group and used to generate cDNA. The relative abundance of MATER and Zar-1 transcripts was analyzed by Real Time PCR. No difference was found on relative expression for MATER and Zar-1 among groups. In conclusion, the conventional morphologic selection associated to the selection by BCB dye can be used as method of selection of immature oocyte, distinguishing two oocytes populations with different development competence, however this double selection did not select more competent oocytes when used only the conventional morphologic assessment. Moreover, the dye is not able to select oocytes with different patterns of expression transcripts MATER and Zar-1.

A eficiência da produção in vitro de embriões (PIV) tem sido limitada pelas condições de cultivo in vitro e baixa competência dos oócitos utilizados na maturação in vitro. A utilização do corante Azul Cresil Brilhante (ACB) associado à seleção morfológica convencional, tem sido descrita como uma alternativa para seleção de oócitos de melhor qualidade. Objetivou-se neste trabalho avaliar o uso do ACB como método de seleção de oócitos imaturos bovinos, utilizando como parâmetros de avaliação o desenvolvimento embrionário e a expressão de genes envolvidos na transição do genoma materno-zigótica. Na primeira etapa, um total de 998 oócitos foram distribuídos em 4 grupos: G1-Controle: oócitos submetidos à maturação sem exposição ao corante ; G2- Controle de incubação (mPBS): oócitos mantidos em mPBS por 1h a temperatura de 38,5 C em ar atmosférico; G3-ACB+: oócitos mantidos em mPBS adicionado de ACB, nas mesmas condições de G2, que apresentaram ao final da incubação citoplasma corado; G4- ACB-: oócitos mantidos em mPBS adicionado de ACB nas mesma condições…

Advisors/Committee Members: Ciro Alexandre Alves Torres, Eduardo Paulino da Costa, Giovanni Ribeiro de Carvalho, João Henrique Moreira Viana, Luiz Sergio de Almeida Camargo.

Subjects/Keywords: Oócito; Expressão gênica; ACB; PRODUCAO ANIMAL; Oocytes; Gene expression; BCB

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mota, G. B. (2008). Desenvolvimento e expressão gênica em oócitos bovinos imaturos selecionados por Azul Cresil Brilhante. (Thesis). Universidade Federal de Viçosa. Retrieved from http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=1345

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mota, Gustavo Bruno. “Desenvolvimento e expressão gênica em oócitos bovinos imaturos selecionados por Azul Cresil Brilhante.” 2008. Thesis, Universidade Federal de Viçosa. Accessed March 03, 2021. http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=1345.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mota, Gustavo Bruno. “Desenvolvimento e expressão gênica em oócitos bovinos imaturos selecionados por Azul Cresil Brilhante.” 2008. Web. 03 Mar 2021.

Vancouver:

Mota GB. Desenvolvimento e expressão gênica em oócitos bovinos imaturos selecionados por Azul Cresil Brilhante. [Internet] [Thesis]. Universidade Federal de Viçosa; 2008. [cited 2021 Mar 03]. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=1345.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mota GB. Desenvolvimento e expressão gênica em oócitos bovinos imaturos selecionados por Azul Cresil Brilhante. [Thesis]. Universidade Federal de Viçosa; 2008. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=1345

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Texas Southwestern Medical Center

19. Evaul, Kristen Elizabeth. Gonadotropin-induced Steroidogenesis and Downstream Signals Leading to Oocyte Maturation.

Degree: 2009, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/639

► The Hammes laboratory is interested in understanding the process of steroid-mediated oocyte maturation. This includes examining both steroid production and steroid signaling. In these studies,… (more)

▼ The Hammes laboratory is interested in understanding the process of steroid-mediated oocyte maturation. This includes examining both steroid production and steroid signaling. In these studies, gonadotropin-induced steroid production was examined in the gonads using mouse models, as well as steroid-induced oocyte maturation in frog models. cAMP signaling is known to be important for steroid production, but further downstream pathways were not well characterized. These studies illuminate other downstream signaling pathways triggered by luteinizing hormone (LH) that regulate steroid production in the testes using Leydig cells, which are the primary steroidogenic cells in the testes. A novel downstream pathway was found involving epidermal growth factor receptor (EGFR) transactivation, downstream mitogen-activated protein kinase (MAPK) and steroidogenic acute regulatory protein (StAR) activation that was essential for short, but not long-term LH-induced steroidogenesis in MLTC-1 and primary mouse leydig cells. Despite this discrepancy in vitro, EGFR signaling was required in vivo for testicular testosterone production. To study the effects of steroids on oocyte maturation, the Xenopus laevis frog model was used. It has been shown that G-beta gamma, as well as other signals, keep the oocyte in meiotic arrest. Steroids block this constitutive signal, leading to oocyte maturation. To directly measure rapid changes in G-beta gamma signaling in oocytes, G-beta gamma sensitive-inward rectifying potassium channel currents (GIRKS) were exogenously expressed in Xenopus oocytes. Adding testosterone, the physiologic mediator of oocyte maturation in Xenopus, decreased the G-beta gamma mediated signal. This happened rapidly supporting the well known idea that maturation is a transcription-independent process. It was also seen that the classical androgen receptor (AR) was being used for this process. When the AR was knocked down, testosterone could only decrease GIRK signal at higher concentrations. This showed that testosterone is working, at least partially, through the AR. These studies may help elucidate novel targets for polycystic ovary syndrome (PCOS), which is characterized by excess androgen due to improper steroid production. Advisors/Committee Members: Hammes, Stephen R..

Subjects/Keywords: Oocytes; Receptors, Steroid; Testosterone

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Evaul, K. E. (2009). Gonadotropin-induced Steroidogenesis and Downstream Signals Leading to Oocyte Maturation. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/639

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Evaul, Kristen Elizabeth. “Gonadotropin-induced Steroidogenesis and Downstream Signals Leading to Oocyte Maturation.” 2009. Thesis, University of Texas Southwestern Medical Center. Accessed March 03, 2021. http://hdl.handle.net/2152.5/639.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Evaul, Kristen Elizabeth. “Gonadotropin-induced Steroidogenesis and Downstream Signals Leading to Oocyte Maturation.” 2009. Web. 03 Mar 2021.

Vancouver:

Evaul KE. Gonadotropin-induced Steroidogenesis and Downstream Signals Leading to Oocyte Maturation. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2009. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/2152.5/639.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Evaul KE. Gonadotropin-induced Steroidogenesis and Downstream Signals Leading to Oocyte Maturation. [Thesis]. University of Texas Southwestern Medical Center; 2009. Available from: http://hdl.handle.net/2152.5/639

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Newcastle

20. Lane, Simon I. R. Control of chromosome segregation in mouse oocytes.

Degree: PhD, 2012, University of Newcastle

URL: http://hdl.handle.net/1959.13/932161

►

Research Doctorate - Doctor of Philosophy (PhD)

This thesis explores the first meiotic division in mouse oocytes, using imaging of fluorescent chimeras by confocal and… (more)

Subjects/Keywords: mice; meiosis I; oocytes; aneuploidy; anaphase promoting complex; spindle assembly checkpoint

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lane, S. I. R. (2012). Control of chromosome segregation in mouse oocytes. (Doctoral Dissertation). University of Newcastle. Retrieved from http://hdl.handle.net/1959.13/932161

Chicago Manual of Style (16^th Edition):

Lane, Simon I R. “Control of chromosome segregation in mouse oocytes.” 2012. Doctoral Dissertation, University of Newcastle. Accessed March 03, 2021. http://hdl.handle.net/1959.13/932161.

MLA Handbook (7^th Edition):

Lane, Simon I R. “Control of chromosome segregation in mouse oocytes.” 2012. Web. 03 Mar 2021.

Vancouver:

Lane SIR. Control of chromosome segregation in mouse oocytes. [Internet] [Doctoral dissertation]. University of Newcastle; 2012. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1959.13/932161.

Council of Science Editors:

Lane SIR. Control of chromosome segregation in mouse oocytes. [Doctoral Dissertation]. University of Newcastle; 2012. Available from: http://hdl.handle.net/1959.13/932161

University of South Carolina

21. Kordus, Richard John. Using Human Granulosa Cells to Select the Most Competent Embryos for Uterine Transfer in in Vitro Fertilization Cycles.

Degree: PhD, Biomedical Engineering, 2020, University of South Carolina

URL: https://scholarcommons.sc.edu/etd/5675

► Infertility is a worldwide epidemic often treated with in vitro fertilization. The success of in vitro fertilization is directly dependent upon the quality of… (more)

▼ Infertility is a worldwide epidemic often treated with in vitro fertilization. The success of in vitro fertilization is directly dependent upon the quality of oocytes produced during the controlled stimulation of the patient’s ovaries. There is a need for improved methods to allow embryologists to select the most viable embryos with the highest probability of leading to live births since in vitro fertilization success is far from optimal. Oocyte and subsequent embryo quality are intimately influenced by the granulosa cells which surround the oocyte during follicular maturation. The oocytes require proper signaling and energy production form the surrounding granulosa cells for ideal maturation which will allow the oocyte to fertilize and produce a viable embryo. We hypothesize that mRNA levels of certain genes in granulosa cells will allow for the identification oocytes that will produce euploid embryos and the most viable embryos within a cohort. Secondly, we hypothesize that the rate by which mitochondria from granulosa cells are able to utilize certain metabolic substrates will be able to identify patients that have increased probability of producing high quality embryos leading to live births. This dissertation attempts to identify a group of genes within individual cumulus cells that show differential mRNA gene expression between oocytes of euploid embryos and oocytes leading to live birth compared to oocytes that do not. From these genes we further attempt to create a model using multiple genes to identify the most viable oocytes within a cohort. This dissertation also attempts to identify metabolic substrates differentially utilized by granulosa cell mitochondria based on patient demographics or embryo development and determine if individual substrates can be used as mitochondrial biomarkers for assisted reproduction. As a whole, the work in this dissertation seeks to provide a panel of biomarkers that can be used by clinicians to better identify embryos that will help improve overall assisted reproductive success. Advisors/Committee Members: Holly LaVoie.

Subjects/Keywords: Biomedical Engineering and Bioengineering; Infertility; in vitro fertilization; oocytes; embryologists

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kordus, R. J. (2020). Using Human Granulosa Cells to Select the Most Competent Embryos for Uterine Transfer in in Vitro Fertilization Cycles. (Doctoral Dissertation). University of South Carolina. Retrieved from https://scholarcommons.sc.edu/etd/5675

Chicago Manual of Style (16^th Edition):

Kordus, Richard John. “Using Human Granulosa Cells to Select the Most Competent Embryos for Uterine Transfer in in Vitro Fertilization Cycles.” 2020. Doctoral Dissertation, University of South Carolina. Accessed March 03, 2021. https://scholarcommons.sc.edu/etd/5675.

MLA Handbook (7^th Edition):

Kordus, Richard John. “Using Human Granulosa Cells to Select the Most Competent Embryos for Uterine Transfer in in Vitro Fertilization Cycles.” 2020. Web. 03 Mar 2021.

Vancouver:

Kordus RJ. Using Human Granulosa Cells to Select the Most Competent Embryos for Uterine Transfer in in Vitro Fertilization Cycles. [Internet] [Doctoral dissertation]. University of South Carolina; 2020. [cited 2021 Mar 03]. Available from: https://scholarcommons.sc.edu/etd/5675.

Council of Science Editors:

Kordus RJ. Using Human Granulosa Cells to Select the Most Competent Embryos for Uterine Transfer in in Vitro Fertilization Cycles. [Doctoral Dissertation]. University of South Carolina; 2020. Available from: https://scholarcommons.sc.edu/etd/5675

University of Texas Southwestern Medical Center

22. Young, Melissa Rasar. Paxillin is a Novel Regulator of Xenopus Oocyte Maturation.

Degree: 2010, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/786

► Oocyte maturation is triggered by steroids in a transcription-independent fashion that involves an unusual positive feedback loop whereby MOS (a germ cell specific Raf) activates… (more)

Subjects/Keywords: Oocytes; Paxillin; Gene Expression Regulation

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APA (6^th Edition):

Young, M. R. (2010). Paxillin is a Novel Regulator of Xenopus Oocyte Maturation. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/786

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Young, Melissa Rasar. “Paxillin is a Novel Regulator of Xenopus Oocyte Maturation.” 2010. Thesis, University of Texas Southwestern Medical Center. Accessed March 03, 2021. http://hdl.handle.net/2152.5/786.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Young, Melissa Rasar. “Paxillin is a Novel Regulator of Xenopus Oocyte Maturation.” 2010. Web. 03 Mar 2021.

Vancouver:

Young MR. Paxillin is a Novel Regulator of Xenopus Oocyte Maturation. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2010. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/2152.5/786.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Young MR. Paxillin is a Novel Regulator of Xenopus Oocyte Maturation. [Thesis]. University of Texas Southwestern Medical Center; 2010. Available from: http://hdl.handle.net/2152.5/786

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Uppsala University

23. Ekberg, Sara. Cryopreservation of Oocytes : Comparison between the Cryoloop and the Cryopette vitrification techniques.

Degree: Medical Biochemistry and Microbiology, 2010, Uppsala University

URL: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-125951

► Crypreservation of oocytes is recently being considered to be a valid choice in infertility treatments.Low survival and fertilization rates due to inefficient slow freeze… (more)

Subjects/Keywords: Oocytes; cryopreservation; vitrification; Cryoloop; Cryopette

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APA (6^th Edition):

Ekberg, S. (2010). Cryopreservation of Oocytes : Comparison between the Cryoloop and the Cryopette vitrification techniques. (Thesis). Uppsala University. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-125951

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ekberg, Sara. “Cryopreservation of Oocytes : Comparison between the Cryoloop and the Cryopette vitrification techniques.” 2010. Thesis, Uppsala University. Accessed March 03, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-125951.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ekberg, Sara. “Cryopreservation of Oocytes : Comparison between the Cryoloop and the Cryopette vitrification techniques.” 2010. Web. 03 Mar 2021.

Vancouver:

Ekberg S. Cryopreservation of Oocytes : Comparison between the Cryoloop and the Cryopette vitrification techniques. [Internet] [Thesis]. Uppsala University; 2010. [cited 2021 Mar 03]. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-125951.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ekberg S. Cryopreservation of Oocytes : Comparison between the Cryoloop and the Cryopette vitrification techniques. [Thesis]. Uppsala University; 2010. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-125951

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

24. Jiménez Trigos, María Estrella. Generation of offspring from cryopreserved rabbit (Oryctolagus cuniculus) oocytes .

Degree: 2014, Universitat Politècnica de València

URL: http://hdl.handle.net/10251/37977

► The general aim of this thesis was to optimise the current methodologies of oocyte cryopreservation in order to obtain live offspring from cryopreserved rabbit oocytes.… (more)

▼ The general aim of this thesis was to optimise the current methodologies of oocyte cryopreservation in order to obtain live offspring from cryopreserved rabbit oocytes. In chapter 1, meiotic spindle configuration, cortical granules (CGs) distribution and oocyte developmental competence were evaluated after cryopreservation with the current slow-freezing and vitrification procedures. The meiotic spindle organisation was dramatically impaired regardless of the method used. Nevertheless, altered CG distribution is more evident in vitrified oocytes than in slow-frozen ones and the developmental rate to blastocyst stage after parthenogenetic activation was only obtained using slow-freezing method. From this chapter it may be concluded that both methodologies equally affect oocyte structure. However, slow-freezing method seems to be the recommended option for this species as a consequence of the sensitivity to high levels of cryoprotectants in this species. The aim of the following two chapters was the optimisation of cryopreservation procedures using different strategies to modify the oocytes in order to make them more cryoresistant. In chapter 2, Taxol and Cytochalasin B were employed to stabilise the cytoskeleton system during vitrification. The effect of these two molecules on the meiotic spindle and chromosome configuration and development to blastocyst stage after parthenogenesis activation were also evaluated. There were no significant differences in the structural configuration between vitrified groups. Regarding cleavage and blastocyst developmental rate, no statistical differences were found between vitrified-non-treated and Taxol-treated oocytes, but no oocytes treated with Cytochalasin B reached this stage. Therefore, structural configuration and blastocyst development were not improved by this pre-treatment. Moreover, Cytochalasin B pre-treatment seems to cause a deleterious effect on developmental ability to blastocyst stage of these oocytes. In chapter 3, oocytes were incubated with cholesterol-loaded methyl-ß- cyclodextrin (CLC) to increase the membrane fluidity and stability and improve their developmental ability after parthenogenetic activation or intracytoplasmic sperm injection (ICSI). Cholesterol incorporation and its presence after cryopreservation were evaluated using confocal microscopy. Results showed that cholesterol was incorporated into the oocyte and remained, albeit in a lesser amount after cryopreservation procedures. However, no improvements on developmental competence were obtained after parthenogenetic activation or intracytoplasmic sperm injection. In the last three chapters of this thesis, the main objective was to develop a reliable technique which would allow us to obtain live offspring from cryopreserved oocytes. For that purpose, in vivo fertilisation using intraoviductal oocyte transfer assisted by laparoscopy was considered a good alternative to bypass the inadequacy of conventional in vitro… Advisors/Committee Members: Marco Jiménez, Francisco (advisor), Vicente Antón, José Salvador (advisor).

Subjects/Keywords: Cryopreservation; Vitrification; Slow-freezing; Oocytes; Rabbit; Offspring; In vivo fertilisation; Laparoscopy

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jiménez Trigos, M. E. (2014). Generation of offspring from cryopreserved rabbit (Oryctolagus cuniculus) oocytes . (Doctoral Dissertation). Universitat Politècnica de València. Retrieved from http://hdl.handle.net/10251/37977

Chicago Manual of Style (16^th Edition):

Jiménez Trigos, María Estrella. “Generation of offspring from cryopreserved rabbit (Oryctolagus cuniculus) oocytes .” 2014. Doctoral Dissertation, Universitat Politècnica de València. Accessed March 03, 2021. http://hdl.handle.net/10251/37977.

MLA Handbook (7^th Edition):

Jiménez Trigos, María Estrella. “Generation of offspring from cryopreserved rabbit (Oryctolagus cuniculus) oocytes .” 2014. Web. 03 Mar 2021.

Vancouver:

Jiménez Trigos ME. Generation of offspring from cryopreserved rabbit (Oryctolagus cuniculus) oocytes . [Internet] [Doctoral dissertation]. Universitat Politècnica de València; 2014. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10251/37977.

Council of Science Editors:

Jiménez Trigos ME. Generation of offspring from cryopreserved rabbit (Oryctolagus cuniculus) oocytes . [Doctoral Dissertation]. Universitat Politècnica de València; 2014. Available from: http://hdl.handle.net/10251/37977

University of Minnesota

25. Olker, Jennifer. Effects of atrazine and climate change on amphibian larval development and growth.

Degree: PhD, Integrated Biosciences, 2014, University of Minnesota

URL: http://hdl.handle.net/11299/191478

► The distribution and population persistence of many North American amphibians depends on environmental factors at multiple spatial scales. Anthropogenic and naturally occurring stressors, including contaminants,… (more)

▼ The distribution and population persistence of many North American amphibians depends on environmental factors at multiple spatial scales. Anthropogenic and naturally occurring stressors, including contaminants, predators, and pond-drying, have been shown to affect amphibian growth, development, and health. The herbicide atrazine (2-chloro-4-ethylamino-6-isoproyl-amino-s-triazine) is a widely used pesticide in the U.S., and in some amphibians has been shown to reduce size and health at metamorphosis and alter gonadal function, presumably through endocrine disruption. Environmental changes predicted by climate models could exacerbate these impacts, as well as directly affect amphibian development and population persistence through accelerated pond-drying and habitat loss or modification. Objectives of this project were to: 1) Quantify developmental responses to the combined effects of atrazine exposure and accelerated pond-drying rates; and 2) Quantify potential effects of these and other environmental stressors on amphibian occurrence and health. Growth, development, and physiological state (skeletal/eye malformations and gonadal development) were assessed in northern leopard frog (Rana pipiens) and wood frog (Rana sylvatica) in experimental exposures and field surveys in the U.S. Prairie Pothole Region across a range of environmentally relevant atrazine concentrations (0.1, 20, and 200 μg/L) and in combination with climate change and other environmental factors suspected to affect amphibian larval development. Atrazine exposure during larval development decreased survival and had sub-lethal impacts on growth and development, which could negatively impact populations by reducing annual recruitment and survival of juveniles. Presence, abundance, and severity of testicular oocytes (TOs) did not appear to be related to atrazine exposure in experimental or field specimens; however, TO prevalence differed greatly between species (>40% in R. pipiens and <5% in R. sylvatica). These results suggest that TOs are not likely due solely to endocrine disruption by atrazine and more research is needed to understand reproductive or population-level impacts of TOs. Amphibian metrics (presence, breeding, skeletal malformations, and TOs) responded differently to environmental variables from wetland, local, and landscape scales, and amphibian breeding (presence or success) was identified as a better indicator of environmental condition than species presence, calling, or TOs.

Subjects/Keywords: anurans; atrazine; mesocosms; Prairie Pothole Region; testicular oocytes; wetlands

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Olker, J. (2014). Effects of atrazine and climate change on amphibian larval development and growth. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/191478

Chicago Manual of Style (16^th Edition):

Olker, Jennifer. “Effects of atrazine and climate change on amphibian larval development and growth.” 2014. Doctoral Dissertation, University of Minnesota. Accessed March 03, 2021. http://hdl.handle.net/11299/191478.

MLA Handbook (7^th Edition):

Olker, Jennifer. “Effects of atrazine and climate change on amphibian larval development and growth.” 2014. Web. 03 Mar 2021.

Vancouver:

Olker J. Effects of atrazine and climate change on amphibian larval development and growth. [Internet] [Doctoral dissertation]. University of Minnesota; 2014. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/11299/191478.

Council of Science Editors:

Olker J. Effects of atrazine and climate change on amphibian larval development and growth. [Doctoral Dissertation]. University of Minnesota; 2014. Available from: http://hdl.handle.net/11299/191478

University of Southern California

26. Popova, Maya S. Sites of ethanol action in P2X4 receptors.

Degree: PhD, Pharmaceutical Sciences, 2011, University of Southern California

URL: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/654259/rec/5878

► P2X receptors (P2XRs) are a distinct family of ligand-gated ion channels that are widely found throughout the peripheral and central nervous systems. They are fast… (more)

Subjects/Keywords: alcohol; electrophysiology; ion channels; purinergic P2X4 receptors; xenopus oocytes ❧

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Popova, M. S. (2011). Sites of ethanol action in P2X4 receptors. (Doctoral Dissertation). University of Southern California. Retrieved from http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/654259/rec/5878

Chicago Manual of Style (16^th Edition):

Popova, Maya S. “Sites of ethanol action in P2X4 receptors.” 2011. Doctoral Dissertation, University of Southern California. Accessed March 03, 2021. http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/654259/rec/5878.

MLA Handbook (7^th Edition):

Popova, Maya S. “Sites of ethanol action in P2X4 receptors.” 2011. Web. 03 Mar 2021.

Vancouver:

Popova MS. Sites of ethanol action in P2X4 receptors. [Internet] [Doctoral dissertation]. University of Southern California; 2011. [cited 2021 Mar 03]. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/654259/rec/5878.

Council of Science Editors:

Popova MS. Sites of ethanol action in P2X4 receptors. [Doctoral Dissertation]. University of Southern California; 2011. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/654259/rec/5878

27. Guglielmino, Maria Rosa. Studio di macchinari molecolari in ovociti umani.

Degree: 2011, Università degli Studi di Catania

URL: http://hdl.handle.net/10761/128

►

Il lavoro presentato in questa tesi ha come oggetto di studio il gamete femminile ed in particolare il trascrittoma dell'ovocita umano. All'interno di questa linea… (more)

Subjects/Keywords: Human oocytes; Gene expression; Molecular machinery; Molecular markers

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Guglielmino, M. R. (2011). Studio di macchinari molecolari in ovociti umani. (Thesis). Università degli Studi di Catania. Retrieved from http://hdl.handle.net/10761/128

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Guglielmino, Maria Rosa. “Studio di macchinari molecolari in ovociti umani.” 2011. Thesis, Università degli Studi di Catania. Accessed March 03, 2021. http://hdl.handle.net/10761/128.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Guglielmino, Maria Rosa. “Studio di macchinari molecolari in ovociti umani.” 2011. Web. 03 Mar 2021.

Vancouver:

Guglielmino MR. Studio di macchinari molecolari in ovociti umani. [Internet] [Thesis]. Università degli Studi di Catania; 2011. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10761/128.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Guglielmino MR. Studio di macchinari molecolari in ovociti umani. [Thesis]. Università degli Studi di Catania; 2011. Available from: http://hdl.handle.net/10761/128

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Edinburgh

28. Zhaunova, Liudmila. Regulation of oocyte-specific chromatin organisation during prophase I by the histone demethylase Kdm5/Lid and other proteins.

Degree: PhD, 2017, University of Edinburgh

URL: http://hdl.handle.net/1842/29007

► In Drosophila oocytes, chromosomes undergo dynamic reorganisation during the prophase of the first meiotic division. This is essential to prepare chromatin for synapsis, recombination and… (more)

▼ In Drosophila oocytes, chromosomes undergo dynamic reorganisation during the prophase of the first meiotic division. This is essential to prepare chromatin for synapsis, recombination and consequent chromosome segregation. The progression of meiotic prophase I is well described, while the molecular mechanisms and regulation of these dramatic chromosomal reorganisations are not well understood. Histone modifying enzymes are major regulators of chromatin structure, however, our knowledge of their roles in meiotic prophase I is still limited. In this work, I investigated the role of the histone demethylase Kdm5/Lid, which removes one of the trimethyl groups at Lys4 of Histone 3 (H3K4me3). I showed that Kdm5/Lid is important for the assembly of the synaptonemal complex, pairing of homologous centromeres, and the karyosome formation. Additionally, Kdm5/Lid promotes crossing over and therefore ensures accurate chromosome segregation. Although loss of Kdm5/Lid dramatically increased the level of H3K4me3 in oocytes, catalytically inactive Kdm5/Lid rescued the above cytological defects. Thereby, I found that Kdm5/Lid regulates chromatin architecture in meiotic prophase I oocytes independently of its demethylase activity. To further identify the regulators of meiotic chromatin organisation during prophase I, I carried out a small-scale RNAi screen for karyosome defects. I found that depletion of ubiquitin ligase components, SkpA, Cul-3 and Ubc-6, disrupted the karyosome formation and the assembly of the synaptonemal complex. The success of the small-scale screen motivated me to initiate the genome-scale RNAi screen for karyosome defects. I found 40 new genes that, when depleted, strongly impaired karyosome morphology. Further studies are required to confirm and elucidate their role in chromatin organisation in oocytes. Overall, my findings have advanced our understanding of the regulation of chromatin reorganisation during oocyte development. Because of the conservation between Drosophila and human meiosis, this study provides novel insights into the regulation of meiotic progression in human oocytes.

Subjects/Keywords: chromosomal reorganisations; Kdm5/Lid; Drosophila; chromosome segregation; H3K4me3; oocytes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zhaunova, L. (2017). Regulation of oocyte-specific chromatin organisation during prophase I by the histone demethylase Kdm5/Lid and other proteins. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/29007

Chicago Manual of Style (16^th Edition):

Zhaunova, Liudmila. “Regulation of oocyte-specific chromatin organisation during prophase I by the histone demethylase Kdm5/Lid and other proteins.” 2017. Doctoral Dissertation, University of Edinburgh. Accessed March 03, 2021. http://hdl.handle.net/1842/29007.

MLA Handbook (7^th Edition):

Zhaunova, Liudmila. “Regulation of oocyte-specific chromatin organisation during prophase I by the histone demethylase Kdm5/Lid and other proteins.” 2017. Web. 03 Mar 2021.

Vancouver:

Zhaunova L. Regulation of oocyte-specific chromatin organisation during prophase I by the histone demethylase Kdm5/Lid and other proteins. [Internet] [Doctoral dissertation]. University of Edinburgh; 2017. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1842/29007.

Council of Science Editors:

Zhaunova L. Regulation of oocyte-specific chromatin organisation during prophase I by the histone demethylase Kdm5/Lid and other proteins. [Doctoral Dissertation]. University of Edinburgh; 2017. Available from: http://hdl.handle.net/1842/29007

Louisiana State University

29. Luster, Sabrina Marie. Cryopreservation of bovine and caprine oocytes by vitrification.

Degree: MS, Animal Sciences, 2004, Louisiana State University

URL: etd-11082004-231115 ; https://digitalcommons.lsu.edu/gradschool_theses/4030

► Cryopreservation of animal oocytes will permit germplasm of valuable or unique females to be preserved for extended times. The objective of this research was to… (more)

▼ Cryopreservation of animal oocytes will permit germplasm of valuable or unique females to be preserved for extended times. The objective of this research was to derive a procedure to cryopreserve bovine oocytes by vitrification to be used as recipients for somatic cell nuclear transfer (SCNT). Caprine oocytes vitrified by the same procedure were assayed by cytological examination of microtubules. In the first two of three experiments, bovine oocytes matured in vitro were vitrified in a mixture of ethylene glycol (EG), dimethylsulfoxide (Me2SO) and trehalose, and then subjected to in vitro fertilization (IVF) or SCNT. For vitrification, oocytes were first exposed to increasing concentrations of EG + Me2SO, placed into the vitrification solution composed of 2.8 M Me2SO + 3.6 M EG + 0.65 M trehalose for 20 sec, immediately loaded onto 20-ìm cryoloops, and finally plunged directly into liquid nitrogen (LN2). Vitrified oocytes were warmed by direct immersion of cryoloops into 0.25 M trehalose prepared in TCM-199 medium at 37°C, rinsed briefly, and then assayed. In Experiment I, of 327 bovine oocytes subjected to IVF after being vitrified, 267 cleaved and 32 (9.8%) formed blastocysts, compared to 32.1% blastocysts for control oocytes. In Experiment II, of 266 bovine oocytes enucleated after vitrification and subjected to SCNT, 248 formed couplets, 152 of which cleaved and 31 (12.5%) developed into blastocysts, compared to 33.0% blastocysts for controls. During the course of Experiment II, 20 of 31 blastocysts derived by SCNT of somatic cells from a Brahman cow into vitrified oocytes were transferred into recipients, resulting in three pregnancies and the birth of one Braham calf that has survived to adulthood. In Experiment III, cytological analysis of caprine oocytes vitrified by the same procedure used for bovine oocytes demonstrated that their microtubules were normal, suggesting that this same procedure can also be used for the former species. The results demonstrate that bovine oocytes can be successfully vitrified and warmed, yielding normal embryos after fertilization or SCNT. Additional research is needed to verify that caprine oocytes vitrified by this method can also develop into kids.

Subjects/Keywords: cryopreservation; vitrification; oocytes; caprine; bovine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Luster, S. M. (2004). Cryopreservation of bovine and caprine oocytes by vitrification. (Masters Thesis). Louisiana State University. Retrieved from etd-11082004-231115 ; https://digitalcommons.lsu.edu/gradschool_theses/4030

Chicago Manual of Style (16^th Edition):

Luster, Sabrina Marie. “Cryopreservation of bovine and caprine oocytes by vitrification.” 2004. Masters Thesis, Louisiana State University. Accessed March 03, 2021. etd-11082004-231115 ; https://digitalcommons.lsu.edu/gradschool_theses/4030.

MLA Handbook (7^th Edition):

Luster, Sabrina Marie. “Cryopreservation of bovine and caprine oocytes by vitrification.” 2004. Web. 03 Mar 2021.

Vancouver:

Luster SM. Cryopreservation of bovine and caprine oocytes by vitrification. [Internet] [Masters thesis]. Louisiana State University; 2004. [cited 2021 Mar 03]. Available from: etd-11082004-231115 ; https://digitalcommons.lsu.edu/gradschool_theses/4030.

Council of Science Editors:

Luster SM. Cryopreservation of bovine and caprine oocytes by vitrification. [Masters Thesis]. Louisiana State University; 2004. Available from: etd-11082004-231115 ; https://digitalcommons.lsu.edu/gradschool_theses/4030

Utah State University

30. Alhojaily, Sameer M. The Effect of Lactation and Energy Status on Gene Expression in the Main Reproductive Tissues of Lactating Dairy Cattle.

Degree: PhD, Animal, Dairy, and Veterinary Sciences, 2019, Utah State University

URL: https://digitalcommons.usu.edu/etd/7588

► Modern high-yielding dairy cows are currently producing far more milk than their ancestors due to a prolonged and intensive genetic selection for milk production… (more)

Subjects/Keywords: Dairy cows; Fertility; Negative energy balance; Oocytes; Embryos; Endometrium; Animal Sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Alhojaily, S. M. (2019). The Effect of Lactation and Energy Status on Gene Expression in the Main Reproductive Tissues of Lactating Dairy Cattle. (Doctoral Dissertation). Utah State University. Retrieved from https://digitalcommons.usu.edu/etd/7588

Chicago Manual of Style (16^th Edition):

Alhojaily, Sameer M. “The Effect of Lactation and Energy Status on Gene Expression in the Main Reproductive Tissues of Lactating Dairy Cattle.” 2019. Doctoral Dissertation, Utah State University. Accessed March 03, 2021. https://digitalcommons.usu.edu/etd/7588.

MLA Handbook (7^th Edition):

Alhojaily, Sameer M. “The Effect of Lactation and Energy Status on Gene Expression in the Main Reproductive Tissues of Lactating Dairy Cattle.” 2019. Web. 03 Mar 2021.

Vancouver:

Alhojaily SM. The Effect of Lactation and Energy Status on Gene Expression in the Main Reproductive Tissues of Lactating Dairy Cattle. [Internet] [Doctoral dissertation]. Utah State University; 2019. [cited 2021 Mar 03]. Available from: https://digitalcommons.usu.edu/etd/7588.

Council of Science Editors:

Alhojaily SM. The Effect of Lactation and Energy Status on Gene Expression in the Main Reproductive Tissues of Lactating Dairy Cattle. [Doctoral Dissertation]. Utah State University; 2019. Available from: https://digitalcommons.usu.edu/etd/7588

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