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You searched for subject:( N acetylcysteamine). One record found.

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University of Delaware

1. Wang, Yiben. Characterization and application of peptidoglycan O-acetyltransferase B utilizing N-acetylcysteamine derivatives .

Degree: 2017, University of Delaware

Bacteria have the natural ability to install protective post-synthetic modifications onto its bacterial peptidoglycan (PG); the coat that is woven into bacterial cell wall. Peptidoglycan O-acetyltransferase B (PatB) catalyzes the O-acetylation of peptidoglycan in Gram (–) bacteria, which aids in bacterial survival, as it prevents autolysins such as lysozyme from cleaving the peptidoglycan at the β-1,4-glycosidic bond between N-acetylmuramic acid (NAM) and N-acetylglucosamine (NAG). In this dissertation, the substrate promiscuity and mechanistic details of PatB’s acetylation function was explored and it was determined that PatB has substrate tolerance for bioorthgonal short N-acetylcysteamine (SNAc) donors. Exploiting this lax specificity, a variety of functionality including azides and alkynes were installed on tri-N-acetylglucosamine (NAG)3, a peptidoglycan mimic, as well as peptidoglycan isolated from various Gram (+) (Bacillus subtilis) and Gram (–) (Escherichia coli, Vibrio parahaemolyticus, and Pseudomonas putida) bacterial species. The bioorthogonal modifications were shown to protect the isolated peptidoglycan against lysozyme degradation in vitro. We further demonstrate that this post-synthetic modification of peptidoglycan can be extended to use click chemistry to fluorescently label the carbohydrate backbone of mature peptidoglycan in whole bacterial cells of Bacillus subtilis. Modifying peptidoglycan post-synthetically can aid in the development of antibiotics and immune modulators by expanding on the current understanding of how the bacterial peptidoglycan is processed by lytic enzymes presented in the innate immune system.

Subjects/Keywords: Pure sciences; Bacteria labeling; N-acetylcysteamine; O-acetylation; PatB; Peptidoglycan

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APA (6th Edition):

Wang, Y. (2017). Characterization and application of peptidoglycan O-acetyltransferase B utilizing N-acetylcysteamine derivatives . (Doctoral Dissertation). University of Delaware. Retrieved from http://udspace.udel.edu/handle/19716/23030

Chicago Manual of Style (16th Edition):

Wang, Yiben. “Characterization and application of peptidoglycan O-acetyltransferase B utilizing N-acetylcysteamine derivatives .” 2017. Doctoral Dissertation, University of Delaware. Accessed September 21, 2019. http://udspace.udel.edu/handle/19716/23030.

MLA Handbook (7th Edition):

Wang, Yiben. “Characterization and application of peptidoglycan O-acetyltransferase B utilizing N-acetylcysteamine derivatives .” 2017. Web. 21 Sep 2019.

Vancouver:

Wang Y. Characterization and application of peptidoglycan O-acetyltransferase B utilizing N-acetylcysteamine derivatives . [Internet] [Doctoral dissertation]. University of Delaware; 2017. [cited 2019 Sep 21]. Available from: http://udspace.udel.edu/handle/19716/23030.

Council of Science Editors:

Wang Y. Characterization and application of peptidoglycan O-acetyltransferase B utilizing N-acetylcysteamine derivatives . [Doctoral Dissertation]. University of Delaware; 2017. Available from: http://udspace.udel.edu/handle/19716/23030

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