subject:( Mycobacterium tuberculosis metabolism 60)

Indian Institute of Science

1. Matange, Nishad. Moonlighting Functions of the Rv0805 Phosphodiesterase from Mycobacterium Tuberculosis.

Degree: 2013, Indian Institute of Science

URL: http://etd.iisc.ernet.in/2005/3424 ; http://etd.iisc.ernet.in/abstracts/4291/G25928-Abs.pdf

► All organisms must sense and respond to their environment in order to survive. The processes that allow a living cell to sense changes in its… (more)

▼ All organisms must sense and respond to their environment in order to survive. The processes that allow a living cell to sense changes in its environment, and respond appropriately are collectively referred to as ‘signal transduction’. Cyclic AMP is a ubiquitously used second messenger molecule that plays diverse roles from hormone signalling in mammalian cells to catabolite repression in enteric bacteria. In several bacterial pathogens such as Pseudomonas aeruginosa, cAMP has also been found to mediate pathogenesis, usually by regulating the production of several virulence factors aiding in colonisation of the host. Cyclic AMP signalling has been suggested to regulate the virulence of the obligate intracellular Mycobacterium tuberculosis. Mycobacteria, including M. tuberculosis, code for a large number of adenylyl cyclases, enzymes that synthesise cAMP. Of the 16 putative adenylyl cyclases encoded by M. tuberculosis H37Rv, 10 have received extensive biochemical attention. A knockout of one of these cyclases, Rv0386, resulted in compromised virulence of M. tuberculosis. Ten proteins predicted to bind cAMP and mediate its cellular roles have also been identified in M. tuberculosis. Among these are the cAMP-regulated transcription factor, CRPMt, and cAMP-regulated protein acetyl transferase, KATmt. Comparatively little information is available, however, regarding the roles of cAMP-degrading machinery in mycobacteria. Two phosphodiesterases, with modest activity against cAMP in vitro, have been identified from M. tuberculosis, and are encoded by the Rv0805 and Rv2795c loci. Of these, Rv2795c has orthologs in all sequenced mycobacterial genomes. However, Rv0805-like proteins are coded only by slow growing mycobacteria such as the M. tuberculosis-complex, M. marinum and M. leprae, several of which are human or animal pathogens. Rv0805 belongs to the metallophosphoesterase superfamily of proteins, consisting of metal-dependent phosphoesterases with substrates ranging from large polymers like nucleic acids to small molecules like cAMP and glycerophospholipids. Like other metallophosphoesterases, Rv0805 displays promiscuous substrate utilisation and efficient hydrolysis of 2’3’-cAMP in vitro. Rv0805 also hydrolyses 3’5’-cAMP in vitro. Overexpression of Rv0805 is reported to lead to reduction in intracellular cAMP levels in M. smegmatis and M. tuberculosis, suggesting that it is capable of hydrolysing cAMP in the bacterial cell as well. The structure of Rv0805 revealed a sandwich-like α/β fold, typical of metallophosphoesterases, along with a relatively flexible C-terminal domain of unknown function. Despite extensive biochemical and structural information on Rv0805 however, its roles in mycobacteria remain unknown. In this study, the cellular roles of Rv0805 are explored and using information from biochemical and structural analyses, novel activities and interactions of Rv0805 have been identified. Rv0805, when expressed in M. smegmatis, led to a reduction in intracellular cAMP, as previously reported. However, the… Advisors/Committee Members: Visweswariah, Sandhya S.

Subjects/Keywords: Mycobacterium Tuberculosis; Mycobacterial Signalling; Mycobacterial Pathogenesis; Cyclic AMP Signalling, Mycobacteria; Rv0805 Phosphodiesterase; Metallophophoesterases; Cyclic AMP Metabolism - Mycobacteria; Rv0805 - Mycobacterium Tuberculosis; Mycobacterial Cell Wall Physiology; Signal Transduction - Mycobacteria; Bacteriology

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Matange, N. (2013). Moonlighting Functions of the Rv0805 Phosphodiesterase from Mycobacterium Tuberculosis. (Thesis). Indian Institute of Science. Retrieved from http://etd.iisc.ernet.in/2005/3424 ; http://etd.iisc.ernet.in/abstracts/4291/G25928-Abs.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Matange, Nishad. “Moonlighting Functions of the Rv0805 Phosphodiesterase from Mycobacterium Tuberculosis.” 2013. Thesis, Indian Institute of Science. Accessed December 11, 2019. http://etd.iisc.ernet.in/2005/3424 ; http://etd.iisc.ernet.in/abstracts/4291/G25928-Abs.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Matange, Nishad. “Moonlighting Functions of the Rv0805 Phosphodiesterase from Mycobacterium Tuberculosis.” 2013. Web. 11 Dec 2019.

Vancouver:

Matange N. Moonlighting Functions of the Rv0805 Phosphodiesterase from Mycobacterium Tuberculosis. [Internet] [Thesis]. Indian Institute of Science; 2013. [cited 2019 Dec 11]. Available from: http://etd.iisc.ernet.in/2005/3424 ; http://etd.iisc.ernet.in/abstracts/4291/G25928-Abs.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Matange N. Moonlighting Functions of the Rv0805 Phosphodiesterase from Mycobacterium Tuberculosis. [Thesis]. Indian Institute of Science; 2013. Available from: http://etd.iisc.ernet.in/2005/3424 ; http://etd.iisc.ernet.in/abstracts/4291/G25928-Abs.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. Sreejit G. Functional characterization of Mycobacterium tuberculosis proteins involved in modulating macrophage functions.

Degree: – , 2012, Manipal University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/5430

Included

Summary p. 164-169 and Bibliography p. 182-210

Advisors/Committee Members: Mukhopadhyay, Sangita, Khosla, Sanjeev.

Subjects/Keywords: Mycobacterium tuberculosis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

G, S. (2012). Functional characterization of Mycobacterium tuberculosis proteins involved in modulating macrophage functions. (Thesis). Manipal University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/5430

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

G, Sreejit. “Functional characterization of Mycobacterium tuberculosis proteins involved in modulating macrophage functions.” 2012. Thesis, Manipal University. Accessed December 11, 2019. http://shodhganga.inflibnet.ac.in/handle/10603/5430.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

G, Sreejit. “Functional characterization of Mycobacterium tuberculosis proteins involved in modulating macrophage functions.” 2012. Web. 11 Dec 2019.

Vancouver:

G S. Functional characterization of Mycobacterium tuberculosis proteins involved in modulating macrophage functions. [Internet] [Thesis]. Manipal University; 2012. [cited 2019 Dec 11]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/5430.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

G S. Functional characterization of Mycobacterium tuberculosis proteins involved in modulating macrophage functions. [Thesis]. Manipal University; 2012. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/5430

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

3. Balaji, V. Expression patterns induced by stress in mycobacterium tuberculosis: a genome wide analysis focusing on toxin antitoxin loc; -.

Degree: Microbiology, 2013, University of Delhi

URL: http://shodhganga.inflibnet.ac.in/handle/10603/26615

Available

Appendix p.211-222

Advisors/Committee Members: Gupta, Amita.

Subjects/Keywords: Mycobacterium; Tuberculosis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Balaji, V. (2013). Expression patterns induced by stress in mycobacterium tuberculosis: a genome wide analysis focusing on toxin antitoxin loc; -. (Thesis). University of Delhi. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/26615

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Balaji, V. “Expression patterns induced by stress in mycobacterium tuberculosis: a genome wide analysis focusing on toxin antitoxin loc; -.” 2013. Thesis, University of Delhi. Accessed December 11, 2019. http://shodhganga.inflibnet.ac.in/handle/10603/26615.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Balaji, V. “Expression patterns induced by stress in mycobacterium tuberculosis: a genome wide analysis focusing on toxin antitoxin loc; -.” 2013. Web. 11 Dec 2019.

Vancouver:

Balaji V. Expression patterns induced by stress in mycobacterium tuberculosis: a genome wide analysis focusing on toxin antitoxin loc; -. [Internet] [Thesis]. University of Delhi; 2013. [cited 2019 Dec 11]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/26615.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Balaji V. Expression patterns induced by stress in mycobacterium tuberculosis: a genome wide analysis focusing on toxin antitoxin loc; -. [Thesis]. University of Delhi; 2013. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/26615

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

4. Lakshmanan, J. Glycogenome Reannotation and identification of novel glycoproteins from mycobacterium tuberculosis H37rv; -.

Degree: Biotechnology, 2013, Anna University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/22107

►

Mycobacterium tuberculosis causative agent of human tuberculosis newline TB was responsible for nearly 1 5 million deaths annually accounting newlinealmost 2 4 deaths worldwide The… (more)

▼

Mycobacterium tuberculosis causative agent of human tuberculosis newline TB was responsible for nearly 1 5 million deaths annually accounting newlinealmost 2 4 deaths worldwide The co infection with HIV the evolution of newlinemulti drug resistance MDR extreme drug resistance XDR and more newlinerecently totally drug resistance TDR strains severely hamper the newlineanti tuberculosis therapy The Cell wall associated and secreted newlineglycoconjugates including Lipoarabinomannan LAM Lipomannan LM newlineArabinogalactans AG Phosphoinosidemannoside PIM Cord factor newlineCapsular glucans and Culture filtrate glycoproteins plays an important role in newlinevirulence and pathogenesis of M tuberculosis The current understanding of newlineenzymes involved in the biosynthesis of these functionally important newlineglycoconjugates termed as carbohydrate glycan modifying enzymes newline glycogenome or glycogenes was not sufficient due to lack apparent function newlineassigned to nearly 40 of the coding sequences in M tuberculosis genome newlineHere we report the use of comprehensive Bioinformatics workflow for newlinereannotation of all unknown function proteins from M tuberculosis H37Rv newlinegenome Our analysis predicted 106 novel putative glycan modifying genes newlineFurther non homology based gene cluster analysis was used to predict newlineputative N Glycosylation pathway newlineApart from all the above glycoconjugates M tuberculosis might newlinealso employs several cell wall glycoproteins whose role in virulence and newlinepathogenesis was poorly understood The specific carbohydrate binding newlinelectins immobilized on agarose beads was used to capture cell wall newlineglycoproteins Glyco catch The results showed M tuberculosis cell wall newlinecontains several glycoproteins with terminal mannose ConA PSA and LCU newlineand N Acetylglucosamine DSA Two prominent protein bands eluted from newlinemannose specific ConA lectin was analyzed further using MALDI TOF and newlineESI MS methods The two proteins were identified as hypothetical protein newline 23 KDa and transferase 32

-

Advisors/Committee Members: Rameshkumar, G.

Subjects/Keywords: Glycogenome; Glycoproteins; Mycobacterium; Tuberculosis H37rv

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lakshmanan, J. (2013). Glycogenome Reannotation and identification of novel glycoproteins from mycobacterium tuberculosis H37rv; -. (Thesis). Anna University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/22107

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lakshmanan, J. “Glycogenome Reannotation and identification of novel glycoproteins from mycobacterium tuberculosis H37rv; -.” 2013. Thesis, Anna University. Accessed December 11, 2019. http://shodhganga.inflibnet.ac.in/handle/10603/22107.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lakshmanan, J. “Glycogenome Reannotation and identification of novel glycoproteins from mycobacterium tuberculosis H37rv; -.” 2013. Web. 11 Dec 2019.

Vancouver:

Lakshmanan J. Glycogenome Reannotation and identification of novel glycoproteins from mycobacterium tuberculosis H37rv; -. [Internet] [Thesis]. Anna University; 2013. [cited 2019 Dec 11]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/22107.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lakshmanan J. Glycogenome Reannotation and identification of novel glycoproteins from mycobacterium tuberculosis H37rv; -. [Thesis]. Anna University; 2013. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/22107

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

5. Khan, Arshad. Biochemical studies on some aspects of nitrogen metabolism in mycobacterium sp.

Degree: 2008, University of Pune

URL: http://shodhganga.inflibnet.ac.in/handle/10603/2507

►

The inexorable rise in cases of tuberculosis worldwide fuelled by the HIV epidemic highlights the need for new drugs and particularly those that can shorten… (more)

▼

The inexorable rise in cases of tuberculosis worldwide fuelled by the HIV epidemic highlights the need for new drugs and particularly those that can shorten the duration of treatment. This thesis describes three approaches for the discovery of new novel therapeutic agents against the disease. First approach is the evaluation and characterization of the biochemical role of enzymes related to nitrate metabolism of Mycobacteria during survival in its dormant or latent stage. The second approach is development of a simple screening assay, which could be used to pick up dormant stage specific inhibitors at large scale. The third approach is screening of diverse chemical library to search biologically active molecules against dormant stage of the bacilli. A respiratory type of nitrate reductase, NarGHJI was identified as a first report in Mycobacterium smegmatis during this study. This enzyme’s activity was found to be induced during hypoxic shiftdown of the culture to dormant stage in Wayne’s in vitro model. More significantly blockage of the enzyme’s function by specific inhibitors led to a steep reduction in viability of the bacilli during hypoxic stage survival in Wayne’s in vitro model, which indicated this enzyme as a potential drug target for latent stage. Furthermore, a complete pathway of the assimilation of nitrate was found to be present in M. smegmatis, M. bovis BCG and M. tuberculosis, which also played significant role during its hypoxic stage persistence. Nitrate reductase, NarGHJI also played a significant role during survival of M. tuberculosis within the THP- macrophage environment which indicated the generation of microaerobic environment in the host cells which harbors the pathogen, similar to Wayne’s hypoxic model. Interestingly the essentiality of nitrate reductase, NarGHJI was found irrespective of presence or absence of nitrate in the medium during this intracellular survival of the organism, which indicated the production of nitrate and/ or nitrite in the activated macrophages. We could also use the whole cell activity of nitrate reductase as a reporter system of dormant stage to develop a simple, rapid, and robust cell based high throughput assay for facilitating dormant stage antitubercular screening.

Abstract includes, References are given chapter wise

Advisors/Committee Members: Sarkar, Dhiman, Ganesh, K N.

Subjects/Keywords: Biotechnology; Biochemical; Tuberculosis; Mycobacterium tuberculosis; Drugs

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Khan, A. (2008). Biochemical studies on some aspects of nitrogen metabolism in mycobacterium sp. (Thesis). University of Pune. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/2507

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Khan, Arshad. “Biochemical studies on some aspects of nitrogen metabolism in mycobacterium sp.” 2008. Thesis, University of Pune. Accessed December 11, 2019. http://shodhganga.inflibnet.ac.in/handle/10603/2507.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Khan, Arshad. “Biochemical studies on some aspects of nitrogen metabolism in mycobacterium sp.” 2008. Web. 11 Dec 2019.

Vancouver:

Khan A. Biochemical studies on some aspects of nitrogen metabolism in mycobacterium sp. [Internet] [Thesis]. University of Pune; 2008. [cited 2019 Dec 11]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/2507.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Khan A. Biochemical studies on some aspects of nitrogen metabolism in mycobacterium sp. [Thesis]. University of Pune; 2008. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/2507

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Jawaharlal Nehru University

6. Gupta Sankalp. Studies on regulatory proteins relevant to phosphate assimilation of Mycobacterium tuberculosis; -.

Degree: Microbiology, 2008, Jawaharlal Nehru University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/32687

None

Bibliography p.79-92

Advisors/Committee Members: Sarkar Dibyendu.

Subjects/Keywords: Mycobacterium tuberculosis; Tuberculosis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sankalp, G. (2008). Studies on regulatory proteins relevant to phosphate assimilation of Mycobacterium tuberculosis; -. (Thesis). Jawaharlal Nehru University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/32687

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sankalp, Gupta. “Studies on regulatory proteins relevant to phosphate assimilation of Mycobacterium tuberculosis; -.” 2008. Thesis, Jawaharlal Nehru University. Accessed December 11, 2019. http://shodhganga.inflibnet.ac.in/handle/10603/32687.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sankalp, Gupta. “Studies on regulatory proteins relevant to phosphate assimilation of Mycobacterium tuberculosis; -.” 2008. Web. 11 Dec 2019.

Vancouver:

Sankalp G. Studies on regulatory proteins relevant to phosphate assimilation of Mycobacterium tuberculosis; -. [Internet] [Thesis]. Jawaharlal Nehru University; 2008. [cited 2019 Dec 11]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/32687.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sankalp G. Studies on regulatory proteins relevant to phosphate assimilation of Mycobacterium tuberculosis; -. [Thesis]. Jawaharlal Nehru University; 2008. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/32687

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Indian Institute of Science

7. Ghosh, Soma. A Multiscale Modeling Study of Iron Homeostasis in Mycrobacterium Tuberculosis.

Degree: 2014, Indian Institute of Science

URL: http://etd.iisc.ernet.in/2005/3519 ; http://etd.iisc.ernet.in/abstracts/4386/G26787-Abs.pdf

► Mycobacterium tuberculosis (M.tb), the causative agent of tuberculosis (TB), has remained the largest killer among infectious diseases for over a century. The increasing emergence of… (more)

▼ Mycobacterium tuberculosis (M.tb), the causative agent of tuberculosis (TB), has remained the largest killer among infectious diseases for over a century. The increasing emergence of drug resistant varieties such as the multidrug resistant (MDR) and extremely drug resistant (XDR) strains are only increasing the global burden of the disease. Available statistics indicate that nearly one-third of the world’s population is infected, where the bacteria remains in the latent state but can reactivate into an actively growing stage to cause disease when the individual is immunocompromised. It is thus immensely important to rethink newer strategies for containing and combating the spread of this disease. Extraction of iron from the host cell is one of the many factors that enable the bacterium to survive in the harsh environments of the host macrophages and promote tuberculosis. Host–pathogen interactions can be interpreted as the battle of two systems, each aiming to overcome the other. From the host’s perspective, iron is essential for diverse processes such as oxygen transport, repression, detoxification and DNA synthesis. Infact, during infection, both the host and the pathogen are known to fight for the available iron, thereby influencing the outcome of the infection. It is of no surprise therefore, that many studies have investigated several components of the iron regulatory machinery of M.tb and the host. However, very few attempts have been made to study the interactions between these components and how such interactions lead to a better adapted phenotype. Such studies require exploration at multiple levels of structural and functional complexity, thereby necessitating the use of a multiscale approach. Systems biology adopts an integrated approach to study and understand the function of biological systems. It involves building large scale models based on individual biochemical interactions, followed by model validation and predictions of the system’s response to perturbations, such as a gene knock-out or exposure to drug. In multiscale modeling, an approach employed in this thesis, a particular biological phenomenon is studied at different spatiotemporal levels. Studying responses at multiple scales provides a broader picture of the communications that occur between a host and pathogen. Moreover, such an analysis also provides valuable insights into how perturbation at a particular level can elicit responses at another level and help in the identification of crucial inter-level communications that can possibly be hindered or activated for a desired physiological outcome. The broad objectives of this thesis was to obtain a comprehensive in silico understanding of mycobacterial iron homeostasis and metabolism, the influence of iron on host-pathogen interactions, identification of key players that mediate such interactions, determination of the molecular consequences of inhibiting the key players and finally the global response of M.tb to altered iron concentration. Perturbation of iron homeostasis holds a strong… Advisors/Committee Members: Vishveshwara, Saraswathi.

Subjects/Keywords: Mycobacterium Tubercolosis; Mycobacterial Iron Homeostasis; Systems Biology; Host-Pathogen Interaction (HPI) Model; Protein Structure Network; Molecular Dynamics; Tuberculosis Infections; Mycobacterium Tuberculosis Iron Metabolism; Protein Interaction Networks; Iron Dependant Repressor; IdeR-DNA Interactions; Tuberculosis - Iron Homeostasis; IdeR; Molecular Biophysics

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ghosh, S. (2014). A Multiscale Modeling Study of Iron Homeostasis in Mycrobacterium Tuberculosis. (Thesis). Indian Institute of Science. Retrieved from http://etd.iisc.ernet.in/2005/3519 ; http://etd.iisc.ernet.in/abstracts/4386/G26787-Abs.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ghosh, Soma. “A Multiscale Modeling Study of Iron Homeostasis in Mycrobacterium Tuberculosis.” 2014. Thesis, Indian Institute of Science. Accessed December 11, 2019. http://etd.iisc.ernet.in/2005/3519 ; http://etd.iisc.ernet.in/abstracts/4386/G26787-Abs.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ghosh, Soma. “A Multiscale Modeling Study of Iron Homeostasis in Mycrobacterium Tuberculosis.” 2014. Web. 11 Dec 2019.

Vancouver:

Ghosh S. A Multiscale Modeling Study of Iron Homeostasis in Mycrobacterium Tuberculosis. [Internet] [Thesis]. Indian Institute of Science; 2014. [cited 2019 Dec 11]. Available from: http://etd.iisc.ernet.in/2005/3519 ; http://etd.iisc.ernet.in/abstracts/4386/G26787-Abs.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ghosh S. A Multiscale Modeling Study of Iron Homeostasis in Mycrobacterium Tuberculosis. [Thesis]. Indian Institute of Science; 2014. Available from: http://etd.iisc.ernet.in/2005/3519 ; http://etd.iisc.ernet.in/abstracts/4386/G26787-Abs.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Indian Institute of Science

8. Rajan Prabhu, J. Structural Studies On Mycobacterial RecA And RuvA.

Degree: 2009, Indian Institute of Science

URL: http://hdl.handle.net/2005/924

► Homologous recombination is a fundamental cellular process evolved to maintain genomic integrity and to generate genetic diversity. It plays a crucial role in DNA repair,… (more)

▼ Homologous recombination is a fundamental cellular process evolved to maintain genomic integrity and to generate genetic diversity. It plays a crucial role in DNA repair, correct segregation of meiotic chromosomes and resumption of the stalled replication forks. In vitro, the homologous recombination pathway is kinetically separable into a four step process involving initiation, homologous pairing, branch migration and junction resolution. The process of pairing and strand exchange between two homologous double-stranded DNA molecules leads to the formation of an intermediate structure called the Holliday junction (HJ). The crucial enzyme involved in this step in bacteria is RecA. In eubacteria, the junction is processed by three proteins, collectively referred to as the RuvABC protein complex. RuvA binds to the HJ, while RuvB, a helicase, binds to the RuvA-HJ complex and pumps the duplex DNA thus facilitating branch migration. The work reported here is concerned with structural studies on mycobacterial RecA and RuvA. X-ray crystallography was used to solve the protein crystal structures. The hanging drop vapour diffusion method was used for crystallization in all cases. X-ray intensity data were collected on a MAR Research imaging plate mounted on a Rigaku RU200 X-ray generator except for two data sets collected using synchrotron radiation. The data were processed mostly using Mosflm and Scala and few data sets were processed using the HKL program suite. The molecular replacement method using programs Phaser and AMoRe was used for structure solution. Structure refinements were carried out using programs CNS and PHENIX. Model building was performed using COOT and O. PROCHECK, MOLPROBITY, ALIGN and NACCESS were used for structure validation and analysis of the refined structures. Mycobacterium smegmatis RecA (MsRecA) and its nucleotide complexes crystallize in three different, but closely related, forms characterized by specific ranges of unit cell dimensions. The six crystals discussed in the earlier part of the thesis and the five reported earlier, all grown under the same or very similar conditions, belong to these three forms, all in space group P61. They include one obtained by reducing the relative humidity around the crystal. In all crystals, RecA monomers form filaments around a 61 screw axis. Thus, the c-dimension of the crystal corresponds to the pitch of the RecA filament. As reported in the case of E.coli RecA, the variation in the pitch among the three forms correlate well with the motion of the C-terminal domain of the RecA monomers with respect to the main domain. The domain motion is compatible with formation of inactive as well as active RecA filaments involving monomers with a fully ordered C-domain. It does not appear to influence the movement upon nucleotide-binding of the switch residue Gln 196, which is believed to provide the trigger for transmitting the effect of nucleotide-binding to the DNA-binding region. Interestingly, partial dehydration of the crystal results in the movement of… Advisors/Committee Members: Vijayan, M.

Subjects/Keywords: Mycobacterium - Recombination; Mycobacterium Smegmatis RecA; Protien; Mycobacterium Tuberculosis RecA; RuvA Protein; Mycobacterial Proteins; Mycobacterial RecA; Mycobacterium Tuberculosis RuvA; Microbiology

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rajan Prabhu, J. (2009). Structural Studies On Mycobacterial RecA And RuvA. (Thesis). Indian Institute of Science. Retrieved from http://hdl.handle.net/2005/924

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rajan Prabhu, J. “Structural Studies On Mycobacterial RecA And RuvA.” 2009. Thesis, Indian Institute of Science. Accessed December 11, 2019. http://hdl.handle.net/2005/924.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rajan Prabhu, J. “Structural Studies On Mycobacterial RecA And RuvA.” 2009. Web. 11 Dec 2019.

Vancouver:

Rajan Prabhu J. Structural Studies On Mycobacterial RecA And RuvA. [Internet] [Thesis]. Indian Institute of Science; 2009. [cited 2019 Dec 11]. Available from: http://hdl.handle.net/2005/924.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rajan Prabhu J. Structural Studies On Mycobacterial RecA And RuvA. [Thesis]. Indian Institute of Science; 2009. Available from: http://hdl.handle.net/2005/924

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

9. Sharma, Ravinder. Molecular Heterogeneity of Mycobacterium tuberculosis Complex Isolates from Punjab India; No.

Degree: Science, 2014, Guru Nanak Dev University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/23441

No newline

Summary: 136-141, References:142-163

Advisors/Committee Members: Sehajpal, Parbodh Kumar.

Subjects/Keywords: Mycobacterium tuberculosis; Punjab

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sharma, R. (2014). Molecular Heterogeneity of Mycobacterium tuberculosis Complex Isolates from Punjab India; No. (Thesis). Guru Nanak Dev University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/23441

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sharma, Ravinder. “Molecular Heterogeneity of Mycobacterium tuberculosis Complex Isolates from Punjab India; No.” 2014. Thesis, Guru Nanak Dev University. Accessed December 11, 2019. http://shodhganga.inflibnet.ac.in/handle/10603/23441.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sharma, Ravinder. “Molecular Heterogeneity of Mycobacterium tuberculosis Complex Isolates from Punjab India; No.” 2014. Web. 11 Dec 2019.

Vancouver:

Sharma R. Molecular Heterogeneity of Mycobacterium tuberculosis Complex Isolates from Punjab India; No. [Internet] [Thesis]. Guru Nanak Dev University; 2014. [cited 2019 Dec 11]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/23441.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sharma R. Molecular Heterogeneity of Mycobacterium tuberculosis Complex Isolates from Punjab India; No. [Thesis]. Guru Nanak Dev University; 2014. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/23441

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

10. Nazia Parveen. Understanding Mycobacterium tuberculosis heat shock protein 60 (Mtbhsp60) mediated modulation of macrophage immune responses.

Degree: DNA, 2014, Manipal University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/17107

Included

Summary p. 128-132 and References p. 133-179, Appendix p. 180-182

Advisors/Committee Members: Mukhopadhyay, Sangita.

Subjects/Keywords: DNA; Mycobacterium tuberculosis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Parveen, N. (2014). Understanding Mycobacterium tuberculosis heat shock protein 60 (Mtbhsp60) mediated modulation of macrophage immune responses. (Thesis). Manipal University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/17107

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Parveen, Nazia. “Understanding Mycobacterium tuberculosis heat shock protein 60 (Mtbhsp60) mediated modulation of macrophage immune responses.” 2014. Thesis, Manipal University. Accessed December 11, 2019. http://shodhganga.inflibnet.ac.in/handle/10603/17107.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Parveen, Nazia. “Understanding Mycobacterium tuberculosis heat shock protein 60 (Mtbhsp60) mediated modulation of macrophage immune responses.” 2014. Web. 11 Dec 2019.

Vancouver:

Parveen N. Understanding Mycobacterium tuberculosis heat shock protein 60 (Mtbhsp60) mediated modulation of macrophage immune responses. [Internet] [Thesis]. Manipal University; 2014. [cited 2019 Dec 11]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/17107.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Parveen N. Understanding Mycobacterium tuberculosis heat shock protein 60 (Mtbhsp60) mediated modulation of macrophage immune responses. [Thesis]. Manipal University; 2014. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/17107

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Hyderabad

11. Yeruva, Chaitanya Veena. Iron limitation in Mycobacterium tuberculosis: studies on iron-regulated envelope proteins & catalase-peroxidases; -.

Degree: Life Science, 2009, University of Hyderabad

URL: http://shodhganga.inflibnet.ac.in/handle/10603/4228

►

Mycobacterium tuberculosis is the causative agent of tuberculosis in humans. Despite several advances in the control of the disease, tuberculosis remains a public concern globally.… (more)

▼

Mycobacterium tuberculosis is the causative agent of tuberculosis in humans. Despite several advances in the control of the disease, tuberculosis remains a public concern globally. A better understanding of host-pathogen interaction is required for effective control measures including diagnosis and development of new and better vaccines. Iron is one of the contributing factors to the virulence of the pathogen. Mycobacteria have adapted to iron deprivation by elaboration of two siderophores, the intracellular mycobactin and extra cellular carboxymycobactin / exochelin and their receptors, the iron-regulated envelope proteins (IREPS). IREPS were first demonstrated in M. smegmatis, with a 29 kDa protein shown to be the ferric-exochelin receptor. Though iron-regulated proteins were identified in M. tuberculosis, no specific envelope proteins were identified upon iron limitation. The genome of M. tuberculosis shows the presence of at least four iron regulators, of which IdeR and FurA are experimentally verified. Though studies with ideR mutants confirmed the role of the IdeR in iron homeostasis, it appears that it is not the sole regulator and that iron-regulation is more complex than envisaged in mycobacteria. FurA, encoded by furA, located immediately upstream of katG encoding catalase-peroxidase controls the expression of KatG, though the association with iron levels remains to be ascertained. The focus of the present study was to understand the effect of iron deprivation in M. tuberculosis and covered two major objectives. First, the effect of iron deprivation on the iron acquisition machinery was analysed and second, the influence of iron levels on catalase-peroxidase was studied. Growth of M. tuberculosis under low iron conditions showed the coordinate expression of both the siderophores and a 28 kDa cell wall-associated iron-regulated protein (Irep-28). Sequence analysis identified Irep-28 as the DNA-binding HU homologue HupB protein (hupB [Rv2986c])..

References include in Chapter 6

Advisors/Committee Members: Sritharan, Manjula.

Subjects/Keywords: Animal Science; Life Science; Mycobacterium tuberculosis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yeruva, C. V. (2009). Iron limitation in Mycobacterium tuberculosis: studies on iron-regulated envelope proteins & catalase-peroxidases; -. (Thesis). University of Hyderabad. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/4228

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yeruva, Chaitanya Veena. “Iron limitation in Mycobacterium tuberculosis: studies on iron-regulated envelope proteins & catalase-peroxidases; -.” 2009. Thesis, University of Hyderabad. Accessed December 11, 2019. http://shodhganga.inflibnet.ac.in/handle/10603/4228.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yeruva, Chaitanya Veena. “Iron limitation in Mycobacterium tuberculosis: studies on iron-regulated envelope proteins & catalase-peroxidases; -.” 2009. Web. 11 Dec 2019.

Vancouver:

Yeruva CV. Iron limitation in Mycobacterium tuberculosis: studies on iron-regulated envelope proteins & catalase-peroxidases; -. [Internet] [Thesis]. University of Hyderabad; 2009. [cited 2019 Dec 11]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/4228.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yeruva CV. Iron limitation in Mycobacterium tuberculosis: studies on iron-regulated envelope proteins & catalase-peroxidases; -. [Thesis]. University of Hyderabad; 2009. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/4228

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

12. Taneja, Bhupesh. Structural studies on the chaperonin 10 of mycobacterium tuberculosis; -.

Degree: Institute of Microbial Technology, 2001, Jawaharlal Nehru University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/30107

None

Reference p.122-126

Advisors/Committee Members: Mande, Shekhar C.

Subjects/Keywords: Chaperonin; Mycobacterium; Tuberculosis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Taneja, B. (2001). Structural studies on the chaperonin 10 of mycobacterium tuberculosis; -. (Thesis). Jawaharlal Nehru University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/30107

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Taneja, Bhupesh. “Structural studies on the chaperonin 10 of mycobacterium tuberculosis; -.” 2001. Thesis, Jawaharlal Nehru University. Accessed December 11, 2019. http://shodhganga.inflibnet.ac.in/handle/10603/30107.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Taneja, Bhupesh. “Structural studies on the chaperonin 10 of mycobacterium tuberculosis; -.” 2001. Web. 11 Dec 2019.

Vancouver:

Taneja B. Structural studies on the chaperonin 10 of mycobacterium tuberculosis; -. [Internet] [Thesis]. Jawaharlal Nehru University; 2001. [cited 2019 Dec 11]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/30107.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Taneja B. Structural studies on the chaperonin 10 of mycobacterium tuberculosis; -. [Thesis]. Jawaharlal Nehru University; 2001. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/30107

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Indian Institute of Science

13. Mishra, Saurabh. Molecular Characterisation Of Mycobacterium Tuberculosis Fic Protein And Its Gene And Identification And Characterisation Of A Novel Functional Interaction Between FtsZ And NDK in Mycobacteria.

Degree: 2012, Indian Institute of Science

URL: http://etd.iisc.ernet.in/handle/2005/2530 ; http://etd.ncsi.iisc.ernet.in/abstracts/3281/G25586-Abs.pdf

► Living organisms employ different kinds of mechanisms, to regulate the functions of genes or their products, which may help in maintaining homeostasis inside the cell… (more)

▼ Living organisms employ different kinds of mechanisms, to regulate the functions of genes or their products, which may help in maintaining homeostasis inside the cell or may help in fighting hostile environment in the case of pathogenic organisms. These mechanisms act at the transcriptional, post-transcriptional, translational, and post-translational levels. In order to understand the physiology of an organism, it is essential to obtain an in-depth knowledge of such mechanisms, in which several proteins participate in interlinked pathways. In this regard, the present study focuses on two such proteins: (i). the newly identified Fic (Filamentation induced by cAMP) protein; and (ii). NDK (Nucleoside Diphosphate Kinase), which had been studied for decades. Fic protein and NDK share several common features: (i). both use nucleoside triphosphate (NTPs) or nucleoside diphosphate (NDPs) or their derivatives as one of their substrates; (ii). they have been found to be involved in diverse cellular pathways, involving different types of substrates that form the second substrate of these proteins; (iii). both are ubiquitously present in all the living organisms - from bacteria to humans to plants. However, there is very little information on these proteins from mycobacterial systems, which include some major human pathogens, Mycobacterium tuberculosis and Mycobacterium leprae, which are the causative agents of Tuberculosis and Leprosy, respectively. In view of these reasons, in the present study, the structural and/or functional features of the Fic and NDK proteins from Mycobacterium tuberculosis, were analysed, as it might be of medical significance for effectively combating the pathogen. The Chapter 1 of the thesis contains the Introduction to the research work and Chapter 2 is on the overall Materials and Methods. The remaining chapters pertain to the data obtained on the structural and/or functional features of the Fic and NDK proteins from Mycobacterium tuberculosis. Chapter 3. Cloning, Expression and Purification of Mycobacterium tuberculosis Fic The role of FIC (Filamentation induced by cAMP) domain containing proteins in the regulation of many vital pathways, mostly through the transfer of NMPs from NTPs to specific target proteins (NMPylylation), in microorganisms, higher eukaryotes, and plants is emerging. In order to understand the biological role of FIC domain containing proteins in mycobacteria, the gene for the FIC domain containing protein of the human pathogen, Mycobacterium tuberculosis, MtuFic, was cloned, overexpressed, purified to homogeneity, and biochemically characterised. Neither the His-tagged nor the GST-tagged MtuFic protein, overexpressed in Escherichia coli, nor expression of Mtufic in Mycobacterium smegmatis, yielded the protein in the soluble fraction. However, the maltose binding protein (MBP) tagged MtuFic (MBP-MtuFic) could be obtained partly in the soluble fraction. Denatured-refolded protein was used for the antibody generation in mice and rabbit. The cellular localisation and secretion of… Advisors/Committee Members: Ajitkumar, Parthasarathi.

Subjects/Keywords: Mycobacterium Tuberculosis - Protein; Mycobacterium Tuberculosis Fic (Filamentation Induced by cAMP) Protein; Mycobacterial FtsZ-NDK Interaction; Mycobaterium Tuberculosis FIC Gene; Mycobacterium Tuberculosis - NDK (Nucleoside Diphosphate Kinase) Proteins; M. tuberculosis; Biochemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mishra, S. (2012). Molecular Characterisation Of Mycobacterium Tuberculosis Fic Protein And Its Gene And Identification And Characterisation Of A Novel Functional Interaction Between FtsZ And NDK in Mycobacteria. (Thesis). Indian Institute of Science. Retrieved from http://etd.iisc.ernet.in/handle/2005/2530 ; http://etd.ncsi.iisc.ernet.in/abstracts/3281/G25586-Abs.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mishra, Saurabh. “Molecular Characterisation Of Mycobacterium Tuberculosis Fic Protein And Its Gene And Identification And Characterisation Of A Novel Functional Interaction Between FtsZ And NDK in Mycobacteria.” 2012. Thesis, Indian Institute of Science. Accessed December 11, 2019. http://etd.iisc.ernet.in/handle/2005/2530 ; http://etd.ncsi.iisc.ernet.in/abstracts/3281/G25586-Abs.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mishra, Saurabh. “Molecular Characterisation Of Mycobacterium Tuberculosis Fic Protein And Its Gene And Identification And Characterisation Of A Novel Functional Interaction Between FtsZ And NDK in Mycobacteria.” 2012. Web. 11 Dec 2019.

Vancouver:

Mishra S. Molecular Characterisation Of Mycobacterium Tuberculosis Fic Protein And Its Gene And Identification And Characterisation Of A Novel Functional Interaction Between FtsZ And NDK in Mycobacteria. [Internet] [Thesis]. Indian Institute of Science; 2012. [cited 2019 Dec 11]. Available from: http://etd.iisc.ernet.in/handle/2005/2530 ; http://etd.ncsi.iisc.ernet.in/abstracts/3281/G25586-Abs.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mishra S. Molecular Characterisation Of Mycobacterium Tuberculosis Fic Protein And Its Gene And Identification And Characterisation Of A Novel Functional Interaction Between FtsZ And NDK in Mycobacteria. [Thesis]. Indian Institute of Science; 2012. Available from: http://etd.iisc.ernet.in/handle/2005/2530 ; http://etd.ncsi.iisc.ernet.in/abstracts/3281/G25586-Abs.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Indian Institute of Science

14. Mishra, Saurabh. Molecular Characterisation Of Mycobacterium Tuberculosis Fic Protein And Its Gene And Identification And Characterisation Of A Novel Functional Interaction Between FtsZ And NDK in Mycobacteria.

Degree: 2012, Indian Institute of Science

URL: http://hdl.handle.net/2005/2530

► Living organisms employ different kinds of mechanisms, to regulate the functions of genes or their products, which may help in maintaining homeostasis inside the cell… (more)

▼ Living organisms employ different kinds of mechanisms, to regulate the functions of genes or their products, which may help in maintaining homeostasis inside the cell or may help in fighting hostile environment in the case of pathogenic organisms. These mechanisms act at the transcriptional, post-transcriptional, translational, and post-translational levels. In order to understand the physiology of an organism, it is essential to obtain an in-depth knowledge of such mechanisms, in which several proteins participate in interlinked pathways. In this regard, the present study focuses on two such proteins: (i). the newly identified Fic (Filamentation induced by cAMP) protein; and (ii). NDK (Nucleoside Diphosphate Kinase), which had been studied for decades. Fic protein and NDK share several common features: (i). both use nucleoside triphosphate (NTPs) or nucleoside diphosphate (NDPs) or their derivatives as one of their substrates; (ii). they have been found to be involved in diverse cellular pathways, involving different types of substrates that form the second substrate of these proteins; (iii). both are ubiquitously present in all the living organisms - from bacteria to humans to plants. However, there is very little information on these proteins from mycobacterial systems, which include some major human pathogens, Mycobacterium tuberculosis and Mycobacterium leprae, which are the causative agents of Tuberculosis and Leprosy, respectively. In view of these reasons, in the present study, the structural and/or functional features of the Fic and NDK proteins from Mycobacterium tuberculosis, were analysed, as it might be of medical significance for effectively combating the pathogen. The Chapter 1 of the thesis contains the Introduction to the research work and Chapter 2 is on the overall Materials and Methods. The remaining chapters pertain to the data obtained on the structural and/or functional features of the Fic and NDK proteins from Mycobacterium tuberculosis. Chapter 3. Cloning, Expression and Purification of Mycobacterium tuberculosis Fic The role of FIC (Filamentation induced by cAMP) domain containing proteins in the regulation of many vital pathways, mostly through the transfer of NMPs from NTPs to specific target proteins (NMPylylation), in microorganisms, higher eukaryotes, and plants is emerging. In order to understand the biological role of FIC domain containing proteins in mycobacteria, the gene for the FIC domain containing protein of the human pathogen, Mycobacterium tuberculosis, MtuFic, was cloned, overexpressed, purified to homogeneity, and biochemically characterised. Neither the His-tagged nor the GST-tagged MtuFic protein, overexpressed in Escherichia coli, nor expression of Mtufic in Mycobacterium smegmatis, yielded the protein in the soluble fraction. However, the maltose binding protein (MBP) tagged MtuFic (MBP-MtuFic) could be obtained partly in the soluble fraction. Denatured-refolded protein was used for the antibody generation in mice and rabbit. The cellular localisation and secretion of… Advisors/Committee Members: Ajitkumar, Parthasarathi.

Subjects/Keywords: Mycobacterium Tuberculosis - Protein; Mycobacterium Tuberculosis Fic (Filamentation Induced by cAMP) Protein; Mycobacterial FtsZ-NDK Interaction; Mycobaterium Tuberculosis FIC Gene; Mycobacterium Tuberculosis - NDK (Nucleoside Diphosphate Kinase) Proteins; M. tuberculosis; Biochemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mishra, S. (2012). Molecular Characterisation Of Mycobacterium Tuberculosis Fic Protein And Its Gene And Identification And Characterisation Of A Novel Functional Interaction Between FtsZ And NDK in Mycobacteria. (Thesis). Indian Institute of Science. Retrieved from http://hdl.handle.net/2005/2530

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mishra, Saurabh. “Molecular Characterisation Of Mycobacterium Tuberculosis Fic Protein And Its Gene And Identification And Characterisation Of A Novel Functional Interaction Between FtsZ And NDK in Mycobacteria.” 2012. Thesis, Indian Institute of Science. Accessed December 11, 2019. http://hdl.handle.net/2005/2530.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mishra, Saurabh. “Molecular Characterisation Of Mycobacterium Tuberculosis Fic Protein And Its Gene And Identification And Characterisation Of A Novel Functional Interaction Between FtsZ And NDK in Mycobacteria.” 2012. Web. 11 Dec 2019.

Vancouver:

Mishra S. Molecular Characterisation Of Mycobacterium Tuberculosis Fic Protein And Its Gene And Identification And Characterisation Of A Novel Functional Interaction Between FtsZ And NDK in Mycobacteria. [Internet] [Thesis]. Indian Institute of Science; 2012. [cited 2019 Dec 11]. Available from: http://hdl.handle.net/2005/2530.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mishra S. Molecular Characterisation Of Mycobacterium Tuberculosis Fic Protein And Its Gene And Identification And Characterisation Of A Novel Functional Interaction Between FtsZ And NDK in Mycobacteria. [Thesis]. Indian Institute of Science; 2012. Available from: http://hdl.handle.net/2005/2530

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Anna University

15. Manickam A. Mycobacterium infection on the function of dendritic cells from patients with cervical cancer;.

Degree: Mycobacterium infection on the function of dendritic cells from patients with cervical cancer, 2014, Anna University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/24191

►

It is now evident that the presentation of tumor antigens by dendritic cells to effector lymphocytes is an important factor for the development of anti… (more)

Subjects/Keywords: Cervical cancer; Dendritic cells; Mycobacterium infection; Mycobacterium tuberculosis; Technology

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

A, M. (2014). Mycobacterium infection on the function of dendritic cells from patients with cervical cancer;. (Thesis). Anna University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/24191

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

A, Manickam. “Mycobacterium infection on the function of dendritic cells from patients with cervical cancer;.” 2014. Thesis, Anna University. Accessed December 11, 2019. http://shodhganga.inflibnet.ac.in/handle/10603/24191.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

A, Manickam. “Mycobacterium infection on the function of dendritic cells from patients with cervical cancer;.” 2014. Web. 11 Dec 2019.

Vancouver:

A M. Mycobacterium infection on the function of dendritic cells from patients with cervical cancer;. [Internet] [Thesis]. Anna University; 2014. [cited 2019 Dec 11]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/24191.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

A M. Mycobacterium infection on the function of dendritic cells from patients with cervical cancer;. [Thesis]. Anna University; 2014. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/24191

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Anna University

16. Elangovan R. Effectiveness of information Education and communication In tuberculosis control;.

Degree: Effectiveness of information Education and communication In tuberculosis control, 2015, Anna University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/41938

►

Tuberculosis TB is a chronic infectious disease primarily caused newlineby the bacillus mycobacterium tuberculosis MTB It mainly spreads by newlineairborne route when the infectious patient… (more)

Subjects/Keywords: Directly Observed Treatment Short; Mycobacterium tuberculosis; Tuberculosis; World Health Organization

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

R, E. (2015). Effectiveness of information Education and communication In tuberculosis control;. (Thesis). Anna University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/41938

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

R, Elangovan. “Effectiveness of information Education and communication In tuberculosis control;.” 2015. Thesis, Anna University. Accessed December 11, 2019. http://shodhganga.inflibnet.ac.in/handle/10603/41938.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

R, Elangovan. “Effectiveness of information Education and communication In tuberculosis control;.” 2015. Web. 11 Dec 2019.

Vancouver:

R E. Effectiveness of information Education and communication In tuberculosis control;. [Internet] [Thesis]. Anna University; 2015. [cited 2019 Dec 11]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/41938.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

R E. Effectiveness of information Education and communication In tuberculosis control;. [Thesis]. Anna University; 2015. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/41938

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Indian Institute of Science

17. Padiadpu, Jyothi. An Integrated Systems Biology Approach to Study Drug Resistance in Mycobacteria.

Degree: 2015, Indian Institute of Science

URL: http://etd.iisc.ernet.in/2005/3750 ; http://etd.iisc.ernet.in/abstracts/4621/G26950-Abs.pdf

► Emergence of drug resistance is a major problem in the treatment of many diseases including tuberculosis. To tackle the problem, it is essential to obtain… (more)

▼ Emergence of drug resistance is a major problem in the treatment of many diseases including tuberculosis. To tackle the problem, it is essential to obtain a global perspective of the molecular mechanisms by which bacteria acquire drug resistance. Systems biology approaches therefore become necessary. This work aims to understand pathways to drug resistance and strategies for inhibition of the resistant strains by using a combination of experimental genomics and computational molecular systems approaches. Laboratory evolution of Mycobacterium smegmatis MC2 155 by treatment with isoniazid (INH), a front-line anti-tubercular drug, resulted in a drug-resistant strain (4XR), capable of growth even at about 10-times the minimum inhibitory concentration of the drug. Whole genome sequence of the 4XR was determined, which indicated only 31 variations in the whole genome, including 3 point mutations, 17 indels and 11 frame-shifts. Two mutations were in proteins required for the pharmacological action of the drug, albeit in regions distant from the drug binding site. The variations however were insufficient to explain the observed resistance to isoniazid. For a better understanding of the global changes associated with drug resistance, whole genome-wide gene expression data was obtained for the resistant strain and compared with that of the WT strain. 716 genes were found to be differentially regulated in 4XR, spanning different biochemical, signaling and regulatory pathways. From this, some explanations for the emergence of drug resistance were obtained, such as the up-regulation of the enzymes in the mycolic acid biosynthesis pathway and also of the drug efflux pumps. In addition, enrichment analysis indicated that up-regulated genes belong to functional categories of response to stress, carbohydrate metabolism, oxidation-reduction process, ion transport, signaling as well as lipid metabolism. The differential gene regulations seemed to be partially responsible for conferring the phenotype to the organism. Alterations in the metabolic pathways in 4XR were characterized using the phenotypic microarray technology, which experimentally scanned the respiratory ability of the resistant bacteria under 280 different nutrient conditions and 96 different inhibitors. Phenotypic gain, where the resistant strain grows significantly better than the wild type and phenotypic loss, where the growth of the resistant strain is compromised as compared to the sensitive strains were derived from the comparison of the phenotypic responses. Differences in survival ability and growth rates in different nutrient sources in the resistant phenotype as compared to the wild type were observed, suggesting rewiring in the metabolic network of the drug-resistant strain. In particular, the pathways of central carbon metabolism and amino acid biosynthesis exhibit significant differences. The strain-specific metabolic pathway differences may guide in devising strategies to tackle the drug-resistant strains selectively and in a rational manner. … Advisors/Committee Members: Chandra, Nagasuma.

Subjects/Keywords: Drug Resistance Tuberculosis; Drug Resistance Mycobacteria; M. smegmatis; MC2 155; Mycobacterium smegmatis; M. tuberculosis; Anti-tubercular Drugs; Mycobacterium tuberculosis; Biochemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Padiadpu, J. (2015). An Integrated Systems Biology Approach to Study Drug Resistance in Mycobacteria. (Thesis). Indian Institute of Science. Retrieved from http://etd.iisc.ernet.in/2005/3750 ; http://etd.iisc.ernet.in/abstracts/4621/G26950-Abs.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Padiadpu, Jyothi. “An Integrated Systems Biology Approach to Study Drug Resistance in Mycobacteria.” 2015. Thesis, Indian Institute of Science. Accessed December 11, 2019. http://etd.iisc.ernet.in/2005/3750 ; http://etd.iisc.ernet.in/abstracts/4621/G26950-Abs.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Padiadpu, Jyothi. “An Integrated Systems Biology Approach to Study Drug Resistance in Mycobacteria.” 2015. Web. 11 Dec 2019.

Vancouver:

Padiadpu J. An Integrated Systems Biology Approach to Study Drug Resistance in Mycobacteria. [Internet] [Thesis]. Indian Institute of Science; 2015. [cited 2019 Dec 11]. Available from: http://etd.iisc.ernet.in/2005/3750 ; http://etd.iisc.ernet.in/abstracts/4621/G26950-Abs.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Padiadpu J. An Integrated Systems Biology Approach to Study Drug Resistance in Mycobacteria. [Thesis]. Indian Institute of Science; 2015. Available from: http://etd.iisc.ernet.in/2005/3750 ; http://etd.iisc.ernet.in/abstracts/4621/G26950-Abs.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Indian Institute of Science

18. Khanduja, Jasbeer Singh. Processing Of DNA Recombination And Replication Intermediates By Mycobacterium Tuberculosis RuvA And RuvB Proteins.

Degree: 2011, Indian Institute of Science

URL: http://hdl.handle.net/2005/2054

► Homologous recombination (HR) is a highly conserved cellular process involved in the maintenance of chromosomal integrity and generation of genetic diversity. Biochemical and genetic studies… (more)

▼ Homologous recombination (HR) is a highly conserved cellular process involved in the maintenance of chromosomal integrity and generation of genetic diversity. Biochemical and genetic studies have suggested that HR is crucial for repair of damaged DNA arising from various endogenous or exogenous assaults on the genome of any organism. Further, HR is vital to repair fatal DNA damage during DNA replication. An instructive example of cross-talk between the processes of DNA recombination and replication can be construed in the processing of replication/recombination/repair intermediates. The impediment(s) to the progression of DNA replication fork is one of the underlying causes for increased genome instability and consequently this might compromise the survival of organism. Various processes manifest at stalled replication forks before they can be rendered competent for the replication-restart. One of the mechanisms of replication-restart involves replication fork reversal (RFR), which envisage unwinding of the blocked forks with simultaneous annealing of the parental and daughter strands o generate a Holliday junction intermediate adjacent to DNA double strand end. Genetic evidence shows that in E. coli dnaEts mutant, holD mutant and in helicase defective rep mutant, RFR is catalyzed by RuvAB complex. Classically, HJ intermediates are generated during the terminal stages of the HR pathway. In E. coli, branch migration and resolution of HJ intermediates is promoted by RuvA, RuvB and RuvC proteins, which participate at the late stages of HR. Structural, biochemical and mutational analysis suggest that E. coli RuvA binds Holliday junction DNA with high affinity and specificity. RuvB, a member of the AAA+ (ATPase associated with various cellular activities) family, is recruited to the RuvA-Holliday junction complex and functions as a motor protein. Together, RuvA and RuvB catalyze ATP dependent branch migration of HJ. The resolution of HJ is catalyzed by the RuvC endonuclease, which introduces coordinated cuts at two symmetrical sites across the junction. RuvAB complex, the Holliday junction branch migration apparatus, is ubiquitous in bacteria. Genetic, biochemical and structural studies have not only established the in vivo role of E. coli RuvAB, in context of HR pathway, but have also provided valuable insights into the mechanism of HJ processing by RuvAB complex. However, the paucity of extensive studies examining the biochemical properties of each member of the RuvABC protein complex restricts models in deciphering the functions of the individual components of this tripartite protein complex. Our current understanding of the biochemical function of E. coli RuvA is within the context of its interacting cellular partner, RuvB. Consequently, the inherent activities of RuvA in the context of DNA repair and HR are poorly understood. Moreover, it remains to be ascertained if RuvABC protein complex, its different sub-complexes, or the individual subunits can function differently in the processing of HJ intermediates… Advisors/Committee Members: Muniyappa, K.

Subjects/Keywords: DNA Recombination; Mycobacterium Tuberculosis; Homologous Recombination (HR); Mycobacterium Tuberculosis RuvA Protein; Mycobacterium Tuberculosis RuvB Protein; DNA Replication; Holliday Junctions; M. tuberculosis; Biochemical Genetics

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Khanduja, J. S. (2011). Processing Of DNA Recombination And Replication Intermediates By Mycobacterium Tuberculosis RuvA And RuvB Proteins. (Thesis). Indian Institute of Science. Retrieved from http://hdl.handle.net/2005/2054

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Khanduja, Jasbeer Singh. “Processing Of DNA Recombination And Replication Intermediates By Mycobacterium Tuberculosis RuvA And RuvB Proteins.” 2011. Thesis, Indian Institute of Science. Accessed December 11, 2019. http://hdl.handle.net/2005/2054.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Khanduja, Jasbeer Singh. “Processing Of DNA Recombination And Replication Intermediates By Mycobacterium Tuberculosis RuvA And RuvB Proteins.” 2011. Web. 11 Dec 2019.

Vancouver:

Khanduja JS. Processing Of DNA Recombination And Replication Intermediates By Mycobacterium Tuberculosis RuvA And RuvB Proteins. [Internet] [Thesis]. Indian Institute of Science; 2011. [cited 2019 Dec 11]. Available from: http://hdl.handle.net/2005/2054.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Khanduja JS. Processing Of DNA Recombination And Replication Intermediates By Mycobacterium Tuberculosis RuvA And RuvB Proteins. [Thesis]. Indian Institute of Science; 2011. Available from: http://hdl.handle.net/2005/2054

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Indian Institute of Science

19. Thakur, Roshan Singh. Role of Mycobacterium Tuberculosis RecG Helicase in DNA Repair, Recombination and in Remodelling of Stalled Replication Forks.

Degree: 2015, Indian Institute of Science

URL: http://etd.iisc.ernet.in/2005/3535 ; http://etd.iisc.ernet.in/abstracts/4403/G27598-Abs.pdf

► Tuberculosis, caused by the infection with Mycobacterium tuberculosis remained as a major global health challenge with one third of world population being infected by this… (more)

▼ Tuberculosis, caused by the infection with Mycobacterium tuberculosis remained as a major global health challenge with one third of world population being infected by this pathogen. M. tuberculosis can persist for decades in infected individuals in the latent state as an asymptomatic disease and can emerge to cause active disease at a later stage. Thus, pathways and the mechanisms that are involved in the maintenance of genome integrity appear to be important for M. tuberculosis survival, persistence and pathogenesis. Helicases are ubiquitous enzymes known to play a key role in DNA replication, repair and recombination. However, role of helicases in providing selective advantage for M. tuberculosis survival and genome maintenance is obscure. Therefore, understanding the role of various helicases could provide insights into the M. tuberculosis survival, persistence and pathogenesis in humans. This information could be useful in considering helicases as a novel therapeutic target as well as developing effective vaccines. The research focus of my thesis has been to understand the role of helicases in safeguarding the M. tuberculosis genome from various genotoxic stresses. The major focus of the current study has been addressed towards understanding the role of M. tuberculosis RecG (MtRecG) helicase in recombinational repair and in remodeling stalled replication forks. This study highlights the importance of RecG helicase in the maintenance of genome integrity via DNA repair, recombination and in remodeling the stalled replication forks in M. tuberculosis. The thesis has been divided into following sections as follows: Chapter I: General introduction that describes the causes and consequences of replication stress and DNA repair pathways in M. tuberculosis The genome is susceptible to various types of damage induced by exogenous as well as endogenous DNA damaging agents. Unrepaired or misrepaired DNA lesions can lead to gross chromosomal rearrangements and ultimately cell death. Thus, organisms have evolved with efficient DNA damage response machinery to cope up with deleterious effects of genotoxic agents. Accurate transmission of genetic information requires error-free duplication of chromosomal DNA during every round of cell division. Defects associated with replication are considered as a major source of genome instability in all organisms. Normal DNA replication is hampered when the fork encounters road blocks that have the potential to stall or collapse a replication fork. The types of lesions that potentially block replication fork include lesions on the template DNA, various secondary structures, R-loops, or DNA bound proteins. To understand the DNA damage induced replication stress and the role of fork remodeling enzymes in the repair of stalled replication forks and its restart, chapter I of the thesis has been distributed into multiple sections as follows: Briefly, initial portion of the chapter describes overall replication process in prokaryotes highlighting the importance of coordinated replisome… Advisors/Committee Members: Nagaraju, Ganesh.

Subjects/Keywords: Mycobacterium Tuberculosis; DNA Replication; DNA Repair; DNA Helicases; DNA Damage; Genomic Instability; Mycobacterium Tuberculosis RecG Helicase; DNA Recombination; M. tuberculosis Genome; Mycobacterium tuberculosis RecG; G4 DNA; Biochemistry

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Thakur, R. S. (2015). Role of Mycobacterium Tuberculosis RecG Helicase in DNA Repair, Recombination and in Remodelling of Stalled Replication Forks. (Thesis). Indian Institute of Science. Retrieved from http://etd.iisc.ernet.in/2005/3535 ; http://etd.iisc.ernet.in/abstracts/4403/G27598-Abs.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Thakur, Roshan Singh. “Role of Mycobacterium Tuberculosis RecG Helicase in DNA Repair, Recombination and in Remodelling of Stalled Replication Forks.” 2015. Thesis, Indian Institute of Science. Accessed December 11, 2019. http://etd.iisc.ernet.in/2005/3535 ; http://etd.iisc.ernet.in/abstracts/4403/G27598-Abs.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Thakur, Roshan Singh. “Role of Mycobacterium Tuberculosis RecG Helicase in DNA Repair, Recombination and in Remodelling of Stalled Replication Forks.” 2015. Web. 11 Dec 2019.

Vancouver:

Thakur RS. Role of Mycobacterium Tuberculosis RecG Helicase in DNA Repair, Recombination and in Remodelling of Stalled Replication Forks. [Internet] [Thesis]. Indian Institute of Science; 2015. [cited 2019 Dec 11]. Available from: http://etd.iisc.ernet.in/2005/3535 ; http://etd.iisc.ernet.in/abstracts/4403/G27598-Abs.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Thakur RS. Role of Mycobacterium Tuberculosis RecG Helicase in DNA Repair, Recombination and in Remodelling of Stalled Replication Forks. [Thesis]. Indian Institute of Science; 2015. Available from: http://etd.iisc.ernet.in/2005/3535 ; http://etd.iisc.ernet.in/abstracts/4403/G27598-Abs.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Indian Institute of Science

20. Roy, Sougata. Transcriptional Analysis Of The Principal Cell Division Gene ftsZ Of Mycobacterium Tuberculosis And Mycobacterium Smegmatis.

Degree: 2006, Indian Institute of Science

URL: http://hdl.handle.net/2005/400

► The success of Mycobacterium tuberculosis as a pathogen is due to its remarkable ability to: (i). adapt to and survive inside activated macrophages under nonproliferating… (more)

▼ The success of Mycobacterium tuberculosis as a pathogen is due to its remarkable ability to: (i). adapt to and survive inside activated macrophages under nonproliferating condition, (ii). put up drug resistance and (iii). enter into hypoxia-induced dormancy and remain in nonproliferating condition, be resistant to drugs, and get reactivated into proliferation when favourable conditions arise. Thus, regulation of cell division (arrest and resumption) is an obligatory event that is critical to the pathogen for the establishment of successful infection, latency and reactivation process in human host. Therefore, in order to understand and combat the successful survival strategy of the bacterium inside the host macrophages or in granuloma, a basic knowledge of the regulation of cell division in tubercle bacillus is essential. Bacterial cytokinetic protein FtsZ (a tubulin homologue) is the key regulatory molecule for cell division and its intracellular level is critical for initiation of cell division in bacteria. Therefore, in order to understand the regulation cell division by expression and maintenance of ftsZ mRNA and protein, we initiated studies on the transcriptional regulation of ftsZ gene in the slow growing pathogen, M. tuberculosis, and in the fast-growing saprophyte M. smegmatis. Identification of regions containing ftsZMt promoter activity In order to identify promoter activity-containing regions of ftsZ gene of M. tuberculosis H37Rv (ftsZMt) in vivo, different regions upstream of ftsZMt namely, the ftsQ-ftsZ intergenic region, the ftsQ open reading frame (ORF), and different regions of ftsQ ORF, were cloned in a gfp reporter plasmid and analyzed for gfp expression in M. smegmatis mc2155 cells. Flow cytometric analysis of exponentially grown M. smegmatis mc2155 cells containing these transcription fusion constructs revealed GFP expression in the cells harbouring ftsQ-ftsZ intergenic region (172 bp), the entire ftsQ ORF (945 bp), and 5’ 467 bp and 3’ 217 bp regions of ftsQ ORF. RT-PCR analyses on RNA from M. smegmatis mc2155 cell transformants carrying the entire ftsQ ORF-ftsQ-ftsZ intergenic region containing construct, as well as on total RNA from M. tuberculosis confirmed that the regions identified indeed elicit promoter activity. RT-PCR analysis on M. tuberculosis RNA as well as semi-quantitative RT-PCR analyses of gfp transcripts driven by cloned MtftsZ promoter regions in M. smegmatis cells showed that about 70% of the total promoter activity comes from ftsQ ORF and there is co-transcription of ftsQ-ftsZ genes. Multiple transcripts code for ftsZMt Primer extension analysis, using primers annealing at different positions in the ftsQ-ftsZ chromosomal region, on RNA from M. tuberculosis as well as from M. smegmatis transformants containing 1.117 kb ftsZMtpromoter region in a promoter probe vector, identified origin of six different transcripts (T1-T6)… Advisors/Committee Members: Ajithkumar, Parthasarathi.

Subjects/Keywords: Gene Transcription; Gene Regulation; Mycobacterium Tuberculosis; Mycobacterium Smegmatis; ftsZ; Cell Division Gene; Molecular Biology

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Roy, S. (2006). Transcriptional Analysis Of The Principal Cell Division Gene ftsZ Of Mycobacterium Tuberculosis And Mycobacterium Smegmatis. (Thesis). Indian Institute of Science. Retrieved from http://hdl.handle.net/2005/400

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Roy, Sougata. “Transcriptional Analysis Of The Principal Cell Division Gene ftsZ Of Mycobacterium Tuberculosis And Mycobacterium Smegmatis.” 2006. Thesis, Indian Institute of Science. Accessed December 11, 2019. http://hdl.handle.net/2005/400.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Roy, Sougata. “Transcriptional Analysis Of The Principal Cell Division Gene ftsZ Of Mycobacterium Tuberculosis And Mycobacterium Smegmatis.” 2006. Web. 11 Dec 2019.

Vancouver:

Roy S. Transcriptional Analysis Of The Principal Cell Division Gene ftsZ Of Mycobacterium Tuberculosis And Mycobacterium Smegmatis. [Internet] [Thesis]. Indian Institute of Science; 2006. [cited 2019 Dec 11]. Available from: http://hdl.handle.net/2005/400.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Roy S. Transcriptional Analysis Of The Principal Cell Division Gene ftsZ Of Mycobacterium Tuberculosis And Mycobacterium Smegmatis. [Thesis]. Indian Institute of Science; 2006. Available from: http://hdl.handle.net/2005/400

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Indian Institute of Science

21. Parimal Kumar, *. Insights Into The Mechanistic Details Of The M.Tuberculosis Pantothenate Kinase : The Key Regulatory Enzyme Of CoA Biosynthesis.

Degree: 2009, Indian Institute of Science

URL: http://hdl.handle.net/2005/1976

► Tuberculosis (TB), caused by Mycobacterium tuberculosis, has long been the scourge of humanity, claiming millions of lives. It is the most devastating infectious disease of… (more)

▼ Tuberculosis (TB), caused by Mycobacterium tuberculosis, has long been the scourge of humanity, claiming millions of lives. It is the most devastating infectious disease of the world in terms of mortality as well as morbidity (WHO, 2009). The lack of a uniformly effective vaccine against TB, the development of resistance in the Mycobacterium tuberculosis against the present antitubercular drugs and its synergy with AIDS has made the situation very alarming. This therefore necessitates a search for new antitubercular drugs as well as the identification of new and unexplored drug targets (Broun et aI., 1992). Coenzyme A is an essential cofactor for all organisms and is synthesized in organisms from pantothenate by a universally conserved pathway (Spry et al., 2008; Sassetti and Rubin, 2003). The first enzyme of the pathway, pantothenate kinase catalyzes the most important step of the biosynthetic process, being the first committed step of CoA biosynthesis and the one at which all the regulation takes place (Gerdes et aI., 2002) This thesis describes the successful cloning of PanK from Mycobacterium tuberculosis, its expression in E. coli, single step affinity purification, and complete biochemical and biophysical characterization. In this work, pantothenol, a widely believed inhibitor of pantothenate kinase, has been shown to act as a substrate for the mycobacterial pantothenate kinase. Further it was shown that the product, 4'phosphopantothenol, thus formed, inhibited the next step of the CoA biosynthesis pathway in vitro. The study was extended to find outthe fate of pantothenol inside the cell and it was demonstrated that the CoA biosynthetic enzymes metabolized the latter into the pantothenol derivative of CoA which then gets incorporated into acyl carrier protein. Lastly, it was decisively shown that pantothenate kinase is not only regulated by feedback inhibition by CoA but, also regulated through feed forward stimulation by Fructose 1, 6 biphosphate (FBP), a glycolytic intermediate. The binding site of FBP was determined by docking and mutational studies of MtPanK. Chapter 1 presents a brief survey of the literature related to Coenzyme A biosynthesis pathway and describes the objective of the thesis. It also presents a history of TB and briefly reviews literature describing TB as well as the life cycle, biology, survival strategy, mode of infection and the metabolic pathways operational in the TB parasite, Mycobacterium tuberculosis. The chapter details the enzymes involved in CoA biosynthesis pathway from various organims. Chapter 2 In this chapter, cloning of the ORF (Rv1092c), annotated as pantothenate kinase in the Tuberculist database (http://genolist.pasteur.frfTubercuList), its expression in E. coli and purification using affinity chromatography has been described. Protein identity was confirmed by MALDI-TOF and by its ability to complement the pantothenate kinase temperature sensitive mutant, DV70. This chapter also illustrates the oligomeric status of MtPanK in solution and describes the… Advisors/Committee Members: Surolia, Avadhesha, Vardarajan, Raghavan.

Subjects/Keywords: Tuberculosis; Micobacterial Pantothenate Kinase; Enzymes - Synthesis; Coenzyme A - Biosynthesis; Enzymes - Regulation; M. tuberculosis; Pantothenate Kinase; Mycobacterium tuberculosis; Pantothenol; Microbiology

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Parimal Kumar, *. (2009). Insights Into The Mechanistic Details Of The M.Tuberculosis Pantothenate Kinase : The Key Regulatory Enzyme Of CoA Biosynthesis. (Thesis). Indian Institute of Science. Retrieved from http://hdl.handle.net/2005/1976

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Parimal Kumar, *. “Insights Into The Mechanistic Details Of The M.Tuberculosis Pantothenate Kinase : The Key Regulatory Enzyme Of CoA Biosynthesis.” 2009. Thesis, Indian Institute of Science. Accessed December 11, 2019. http://hdl.handle.net/2005/1976.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Parimal Kumar, *. “Insights Into The Mechanistic Details Of The M.Tuberculosis Pantothenate Kinase : The Key Regulatory Enzyme Of CoA Biosynthesis.” 2009. Web. 11 Dec 2019.

Vancouver:

Parimal Kumar *. Insights Into The Mechanistic Details Of The M.Tuberculosis Pantothenate Kinase : The Key Regulatory Enzyme Of CoA Biosynthesis. [Internet] [Thesis]. Indian Institute of Science; 2009. [cited 2019 Dec 11]. Available from: http://hdl.handle.net/2005/1976.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Parimal Kumar *. Insights Into The Mechanistic Details Of The M.Tuberculosis Pantothenate Kinase : The Key Regulatory Enzyme Of CoA Biosynthesis. [Thesis]. Indian Institute of Science; 2009. Available from: http://hdl.handle.net/2005/1976

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Indian Institute of Science

22. Parimal Kumar, *. Insights Into The Mechanistic Details Of The M.Tuberculosis Pantothenate Kinase : The Key Regulatory Enzyme Of CoA Biosynthesis.

Degree: 2009, Indian Institute of Science

URL: http://etd.iisc.ernet.in/handle/2005/1976 ; http://etd.ncsi.iisc.ernet.in/abstracts/2561/G23545-Abs.pdf

► Tuberculosis (TB), caused by Mycobacterium tuberculosis, has long been the scourge of humanity, claiming millions of lives. It is the most devastating infectious disease of… (more)

▼ Tuberculosis (TB), caused by Mycobacterium tuberculosis, has long been the scourge of humanity, claiming millions of lives. It is the most devastating infectious disease of the world in terms of mortality as well as morbidity (WHO, 2009). The lack of a uniformly effective vaccine against TB, the development of resistance in the Mycobacterium tuberculosis against the present antitubercular drugs and its synergy with AIDS has made the situation very alarming. This therefore necessitates a search for new antitubercular drugs as well as the identification of new and unexplored drug targets (Broun et aI., 1992). Coenzyme A is an essential cofactor for all organisms and is synthesized in organisms from pantothenate by a universally conserved pathway (Spry et al., 2008; Sassetti and Rubin, 2003). The first enzyme of the pathway, pantothenate kinase catalyzes the most important step of the biosynthetic process, being the first committed step of CoA biosynthesis and the one at which all the regulation takes place (Gerdes et aI., 2002) This thesis describes the successful cloning of PanK from Mycobacterium tuberculosis, its expression in E. coli, single step affinity purification, and complete biochemical and biophysical characterization. In this work, pantothenol, a widely believed inhibitor of pantothenate kinase, has been shown to act as a substrate for the mycobacterial pantothenate kinase. Further it was shown that the product, 4'phosphopantothenol, thus formed, inhibited the next step of the CoA biosynthesis pathway in vitro. The study was extended to find outthe fate of pantothenol inside the cell and it was demonstrated that the CoA biosynthetic enzymes metabolized the latter into the pantothenol derivative of CoA which then gets incorporated into acyl carrier protein. Lastly, it was decisively shown that pantothenate kinase is not only regulated by feedback inhibition by CoA but, also regulated through feed forward stimulation by Fructose 1, 6 biphosphate (FBP), a glycolytic intermediate. The binding site of FBP was determined by docking and mutational studies of MtPanK. Chapter 1 presents a brief survey of the literature related to Coenzyme A biosynthesis pathway and describes the objective of the thesis. It also presents a history of TB and briefly reviews literature describing TB as well as the life cycle, biology, survival strategy, mode of infection and the metabolic pathways operational in the TB parasite, Mycobacterium tuberculosis. The chapter details the enzymes involved in CoA biosynthesis pathway from various organims. Chapter 2 In this chapter, cloning of the ORF (Rv1092c), annotated as pantothenate kinase in the Tuberculist database (http://genolist.pasteur.frfTubercuList), its expression in E. coli and purification using affinity chromatography has been described. Protein identity was confirmed by MALDI-TOF and by its ability to complement the pantothenate kinase temperature sensitive mutant, DV70. This chapter also illustrates the oligomeric status of MtPanK in solution and describes the… Advisors/Committee Members: Surolia, Avadhesha, Vardarajan, Raghavan.

Subjects/Keywords: Tuberculosis; Micobacterial Pantothenate Kinase; Enzymes - Synthesis; Coenzyme A - Biosynthesis; Enzymes - Regulation; M. tuberculosis; Pantothenate Kinase; Mycobacterium tuberculosis; Pantothenol; Microbiology

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Parimal Kumar, *. (2009). Insights Into The Mechanistic Details Of The M.Tuberculosis Pantothenate Kinase : The Key Regulatory Enzyme Of CoA Biosynthesis. (Thesis). Indian Institute of Science. Retrieved from http://etd.iisc.ernet.in/handle/2005/1976 ; http://etd.ncsi.iisc.ernet.in/abstracts/2561/G23545-Abs.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Parimal Kumar, *. “Insights Into The Mechanistic Details Of The M.Tuberculosis Pantothenate Kinase : The Key Regulatory Enzyme Of CoA Biosynthesis.” 2009. Thesis, Indian Institute of Science. Accessed December 11, 2019. http://etd.iisc.ernet.in/handle/2005/1976 ; http://etd.ncsi.iisc.ernet.in/abstracts/2561/G23545-Abs.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Parimal Kumar, *. “Insights Into The Mechanistic Details Of The M.Tuberculosis Pantothenate Kinase : The Key Regulatory Enzyme Of CoA Biosynthesis.” 2009. Web. 11 Dec 2019.

Vancouver:

Parimal Kumar *. Insights Into The Mechanistic Details Of The M.Tuberculosis Pantothenate Kinase : The Key Regulatory Enzyme Of CoA Biosynthesis. [Internet] [Thesis]. Indian Institute of Science; 2009. [cited 2019 Dec 11]. Available from: http://etd.iisc.ernet.in/handle/2005/1976 ; http://etd.ncsi.iisc.ernet.in/abstracts/2561/G23545-Abs.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Parimal Kumar *. Insights Into The Mechanistic Details Of The M.Tuberculosis Pantothenate Kinase : The Key Regulatory Enzyme Of CoA Biosynthesis. [Thesis]. Indian Institute of Science; 2009. Available from: http://etd.iisc.ernet.in/handle/2005/1976 ; http://etd.ncsi.iisc.ernet.in/abstracts/2561/G23545-Abs.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Indian Institute of Science

23. Bharadwaj, Vemparala. Unraveling the Evolutionary Advantages of Crosstalk Between Two-Component Signalling Systems of M tuberculosis.

Degree: 2017, Indian Institute of Science

URL: http://etd.iisc.ernet.in/2005/3636 ; http://etd.iisc.ernet.in/abstracts/4506/G28452-Abs.pdf

► M. tuberculosis (Mtb) senses and responds to changes in its environment primar-ily through two-component signalling systems (TCSs). Each TCS contains a trans-membrane histidine kinase (HK… (more)

▼ M. tuberculosis (Mtb) senses and responds to changes in its environment primar-ily through two-component signalling systems (TCSs). Each TCS contains a trans-membrane histidine kinase (HK ) protein and a cytoplasmic response regulator (RR) protein. HK detects a stimulus and gets phosphorylated. It then binds and transfers the phosphoryl group to the RR of the same TCS. Activated RR then triggers gene ex-pression, including upregulation of the HK and RR involved, eliciting responses that are essential for the bacterium to adapt. Though di erent TCSs detect distinct stimuli, the binding regions of the HK s and RRs share signi cant similarity. This raises the possibil-ity of crosstalk, where HK s dissipate signals to RRs that do not belong to the same TCS. Studies have argued that such dissipation of signals impairs the fitness of the organism, as it decreases the output levels as well as triggers unwanted responses. In contrast, a recent experimental study has discovered that TCSs of Mtb share extensive crosstalk, violating the widely accepted specificity paradigm. In this study, we have attempted to unravel the evolutionary underpinnings of this extensive crosstalk observed in Mtb. We hypothesised that such crosstalk may be advantageous in programmed environments, where there are well-defined sequences of stimuli. In such situations, crosstalk can up-regulate HK s and RRs of non-cognate TCSs. This up-regulation primes the latter TCSs for upcoming signals, increasing their sensitivity. We constructed a mechanistic model of the functioning of TCSs and a fitness variable to qualitatively measure the response of a TCS to a signal, to test the hypothesis. We performed population genetics simulations of the evolution of phenotypes of different crosstalk patterns. We found that in a random environment, the phenotype without any crosstalk is selected over time, which is in agreement with prevalent arguments in favour of specificity of TCSs. But when the environment is programmed, the phenotype with a crosstalk pattern mirroring the pattern of stimuli dominates the population. Finally, we found evidence for the evolutionary preference to preserve crosstalk in gene sequences of HK s and RRs encoded in Mtb. We found that the binding domains of HK s and RRs, which were predicted to share crosstalk, are under greater pressure to be similar than those domains which do not crosstalk. Our study thus provides a plausible explanation of the unexpected presence of crosstalk in Mtb. Since these cross-interactions aid the pathogen to adapt in the host, inhibitors of such interactions are likely to have therapeutic potential. Advisors/Committee Members: Dixit, Narendra M.

Subjects/Keywords: Mycobacterium tuberculosis; Two-component Signalling Systems (TCSs); Trans-Membrane Histidine Kinase; M. tuberculosis (Mtb); Tuberculosis Infection; Chemical Engineering

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bharadwaj, V. (2017). Unraveling the Evolutionary Advantages of Crosstalk Between Two-Component Signalling Systems of M tuberculosis. (Thesis). Indian Institute of Science. Retrieved from http://etd.iisc.ernet.in/2005/3636 ; http://etd.iisc.ernet.in/abstracts/4506/G28452-Abs.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bharadwaj, Vemparala. “Unraveling the Evolutionary Advantages of Crosstalk Between Two-Component Signalling Systems of M tuberculosis.” 2017. Thesis, Indian Institute of Science. Accessed December 11, 2019. http://etd.iisc.ernet.in/2005/3636 ; http://etd.iisc.ernet.in/abstracts/4506/G28452-Abs.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bharadwaj, Vemparala. “Unraveling the Evolutionary Advantages of Crosstalk Between Two-Component Signalling Systems of M tuberculosis.” 2017. Web. 11 Dec 2019.

Vancouver:

Bharadwaj V. Unraveling the Evolutionary Advantages of Crosstalk Between Two-Component Signalling Systems of M tuberculosis. [Internet] [Thesis]. Indian Institute of Science; 2017. [cited 2019 Dec 11]. Available from: http://etd.iisc.ernet.in/2005/3636 ; http://etd.iisc.ernet.in/abstracts/4506/G28452-Abs.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bharadwaj V. Unraveling the Evolutionary Advantages of Crosstalk Between Two-Component Signalling Systems of M tuberculosis. [Thesis]. Indian Institute of Science; 2017. Available from: http://etd.iisc.ernet.in/2005/3636 ; http://etd.iisc.ernet.in/abstracts/4506/G28452-Abs.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

24. Kelkar, Dhanashree S. Proteogenomic analysis of mycobacterium tuberculosis H37Rv; -.

Degree: Biotechnology, 2013, Amrita Vishwa Vidyapeetham (University)

URL: http://shodhganga.inflibnet.ac.in/handle/10603/40979

None

References p.84-88

Advisors/Committee Members: Nair, Bipin G.

Subjects/Keywords: mycobacterium tuberculosis; Proteogenomic analysis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kelkar, D. S. (2013). Proteogenomic analysis of mycobacterium tuberculosis H37Rv; -. (Thesis). Amrita Vishwa Vidyapeetham (University). Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/40979

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kelkar, Dhanashree S. “Proteogenomic analysis of mycobacterium tuberculosis H37Rv; -.” 2013. Thesis, Amrita Vishwa Vidyapeetham (University). Accessed December 11, 2019. http://shodhganga.inflibnet.ac.in/handle/10603/40979.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kelkar, Dhanashree S. “Proteogenomic analysis of mycobacterium tuberculosis H37Rv; -.” 2013. Web. 11 Dec 2019.

Vancouver:

Kelkar DS. Proteogenomic analysis of mycobacterium tuberculosis H37Rv; -. [Internet] [Thesis]. Amrita Vishwa Vidyapeetham (University); 2013. [cited 2019 Dec 11]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/40979.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kelkar DS. Proteogenomic analysis of mycobacterium tuberculosis H37Rv; -. [Thesis]. Amrita Vishwa Vidyapeetham (University); 2013. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/40979

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Jawaharlal Nehru University

25. Srivastava, Sandeep Kumar. Structural studies on NAD dependent DNA ligase Rv3014c from mycobacterium tuberculosis H37Rv; -.

Degree: Pharmacy, 2005, Jawaharlal Nehru University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/23820

None

Bibliography p.122-130

Advisors/Committee Members: Ravisbankar, R.

Subjects/Keywords: Pharmacy; DNA ligase; tuberculosis; mycobacterium

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Srivastava, S. K. (2005). Structural studies on NAD dependent DNA ligase Rv3014c from mycobacterium tuberculosis H37Rv; -. (Thesis). Jawaharlal Nehru University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/23820

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Srivastava, Sandeep Kumar. “Structural studies on NAD dependent DNA ligase Rv3014c from mycobacterium tuberculosis H37Rv; -.” 2005. Thesis, Jawaharlal Nehru University. Accessed December 11, 2019. http://shodhganga.inflibnet.ac.in/handle/10603/23820.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Srivastava, Sandeep Kumar. “Structural studies on NAD dependent DNA ligase Rv3014c from mycobacterium tuberculosis H37Rv; -.” 2005. Web. 11 Dec 2019.

Vancouver:

Srivastava SK. Structural studies on NAD dependent DNA ligase Rv3014c from mycobacterium tuberculosis H37Rv; -. [Internet] [Thesis]. Jawaharlal Nehru University; 2005. [cited 2019 Dec 11]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/23820.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Srivastava SK. Structural studies on NAD dependent DNA ligase Rv3014c from mycobacterium tuberculosis H37Rv; -. [Thesis]. Jawaharlal Nehru University; 2005. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/23820

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Jawaharlal Nehru University

26. Akhtar, Parvez. Molecular characterization of immunodominant antigen of mycobacterium tuberculosis H37Rv; -.

Degree: Pharmacy, 2006, Jawaharlal Nehru University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/23822

None

Bibliography p.123-147 Appendices p.I-X

Advisors/Committee Members: Srivastava, Ranjana.

Subjects/Keywords: Pharmacy; antigen; mycobacterium tuberculosis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Akhtar, P. (2006). Molecular characterization of immunodominant antigen of mycobacterium tuberculosis H37Rv; -. (Thesis). Jawaharlal Nehru University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/23822

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Akhtar, Parvez. “Molecular characterization of immunodominant antigen of mycobacterium tuberculosis H37Rv; -.” 2006. Thesis, Jawaharlal Nehru University. Accessed December 11, 2019. http://shodhganga.inflibnet.ac.in/handle/10603/23822.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Akhtar, Parvez. “Molecular characterization of immunodominant antigen of mycobacterium tuberculosis H37Rv; -.” 2006. Web. 11 Dec 2019.

Vancouver:

Akhtar P. Molecular characterization of immunodominant antigen of mycobacterium tuberculosis H37Rv; -. [Internet] [Thesis]. Jawaharlal Nehru University; 2006. [cited 2019 Dec 11]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/23822.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Akhtar P. Molecular characterization of immunodominant antigen of mycobacterium tuberculosis H37Rv; -. [Thesis]. Jawaharlal Nehru University; 2006. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/23822

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Jawaharlal Nehru University

27. Pulavarti, S V S R Krishna. Solution structure and dynamics of Peptidyl-tRNA Hydrolase from Mycobacterium tuberculosis H37Rv.

Degree: Molecular biology, 2010, Jawaharlal Nehru University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/13470

None

Bibliography and Appendix included

Advisors/Committee Members: Arora, Ashish.

Subjects/Keywords: Molecular biology; Mycobacterium tuberculosis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pulavarti, S. V. S. R. K. (2010). Solution structure and dynamics of Peptidyl-tRNA Hydrolase from Mycobacterium tuberculosis H37Rv. (Thesis). Jawaharlal Nehru University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/13470

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pulavarti, S V S R Krishna. “Solution structure and dynamics of Peptidyl-tRNA Hydrolase from Mycobacterium tuberculosis H37Rv.” 2010. Thesis, Jawaharlal Nehru University. Accessed December 11, 2019. http://shodhganga.inflibnet.ac.in/handle/10603/13470.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pulavarti, S V S R Krishna. “Solution structure and dynamics of Peptidyl-tRNA Hydrolase from Mycobacterium tuberculosis H37Rv.” 2010. Web. 11 Dec 2019.

Vancouver:

Pulavarti SVSRK. Solution structure and dynamics of Peptidyl-tRNA Hydrolase from Mycobacterium tuberculosis H37Rv. [Internet] [Thesis]. Jawaharlal Nehru University; 2010. [cited 2019 Dec 11]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/13470.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pulavarti SVSRK. Solution structure and dynamics of Peptidyl-tRNA Hydrolase from Mycobacterium tuberculosis H37Rv. [Thesis]. Jawaharlal Nehru University; 2010. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/13470

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Jawaharlal Nehru University

28. Kaur, Satinder. Molecular characterization of Mycobacterium tuberculosis H37Rv Protein(s) involved in Persistence/Latency.

Degree: Drug, 2010, Jawaharlal Nehru University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/15764

None

Bibliography p. 94-109, Appendix and Publications included

Advisors/Committee Members: Srivastava, Ranjana.

Subjects/Keywords: Drug; Mycobacterium tuberculosis; Persistence/Latency

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kaur, S. (2010). Molecular characterization of Mycobacterium tuberculosis H37Rv Protein(s) involved in Persistence/Latency. (Thesis). Jawaharlal Nehru University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/15764

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kaur, Satinder. “Molecular characterization of Mycobacterium tuberculosis H37Rv Protein(s) involved in Persistence/Latency.” 2010. Thesis, Jawaharlal Nehru University. Accessed December 11, 2019. http://shodhganga.inflibnet.ac.in/handle/10603/15764.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kaur, Satinder. “Molecular characterization of Mycobacterium tuberculosis H37Rv Protein(s) involved in Persistence/Latency.” 2010. Web. 11 Dec 2019.

Vancouver:

Kaur S. Molecular characterization of Mycobacterium tuberculosis H37Rv Protein(s) involved in Persistence/Latency. [Internet] [Thesis]. Jawaharlal Nehru University; 2010. [cited 2019 Dec 11]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/15764.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kaur S. Molecular characterization of Mycobacterium tuberculosis H37Rv Protein(s) involved in Persistence/Latency. [Thesis]. Jawaharlal Nehru University; 2010. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/15764

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

29. Akhtar, Parvez. Molecular characterization of immunodominant antigen of mycobacterium tuberculosis H37Rv; -.

Degree: Drug Research Institute, 2006, Jawaharlal Nehru University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/28777

non

Bibliography p.123-147 Appendices p.I-X

Advisors/Committee Members: Srivastava, Ranjana.

Subjects/Keywords: immunodominant; antigen; mycobacterium tuberculosis; H37Rv

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Akhtar, P. (2006). Molecular characterization of immunodominant antigen of mycobacterium tuberculosis H37Rv; -. (Thesis). Jawaharlal Nehru University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/28777

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Akhtar, Parvez. “Molecular characterization of immunodominant antigen of mycobacterium tuberculosis H37Rv; -.” 2006. Thesis, Jawaharlal Nehru University. Accessed December 11, 2019. http://shodhganga.inflibnet.ac.in/handle/10603/28777.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Akhtar, Parvez. “Molecular characterization of immunodominant antigen of mycobacterium tuberculosis H37Rv; -.” 2006. Web. 11 Dec 2019.

Vancouver:

Akhtar P. Molecular characterization of immunodominant antigen of mycobacterium tuberculosis H37Rv; -. [Internet] [Thesis]. Jawaharlal Nehru University; 2006. [cited 2019 Dec 11]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/28777.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Akhtar P. Molecular characterization of immunodominant antigen of mycobacterium tuberculosis H37Rv; -. [Thesis]. Jawaharlal Nehru University; 2006. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/28777

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

30. Meher, Akshaya Kumar. Biophysical and biochemical characterization of ESAT 6, CFP 10, and ESAT 6 CFP 10 complex from mycobacterium tuberculosis H37Rv; -.

Degree: Drug Research, 2007, Jawaharlal Nehru University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/29938

None

Abbrevations, Bibilography p.117-129

Advisors/Committee Members: Arora, A.

Subjects/Keywords: Biophysical; mycobacterium; biochemical; heteronuclear; tuberculosis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Meher, A. K. (2007). Biophysical and biochemical characterization of ESAT 6, CFP 10, and ESAT 6 CFP 10 complex from mycobacterium tuberculosis H37Rv; -. (Thesis). Jawaharlal Nehru University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/29938

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Meher, Akshaya Kumar. “Biophysical and biochemical characterization of ESAT 6, CFP 10, and ESAT 6 CFP 10 complex from mycobacterium tuberculosis H37Rv; -.” 2007. Thesis, Jawaharlal Nehru University. Accessed December 11, 2019. http://shodhganga.inflibnet.ac.in/handle/10603/29938.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Meher, Akshaya Kumar. “Biophysical and biochemical characterization of ESAT 6, CFP 10, and ESAT 6 CFP 10 complex from mycobacterium tuberculosis H37Rv; -.” 2007. Web. 11 Dec 2019.

Vancouver:

Meher AK. Biophysical and biochemical characterization of ESAT 6, CFP 10, and ESAT 6 CFP 10 complex from mycobacterium tuberculosis H37Rv; -. [Internet] [Thesis]. Jawaharlal Nehru University; 2007. [cited 2019 Dec 11]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/29938.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Meher AK. Biophysical and biochemical characterization of ESAT 6, CFP 10, and ESAT 6 CFP 10 complex from mycobacterium tuberculosis H37Rv; -. [Thesis]. Jawaharlal Nehru University; 2007. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/29938

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Open Access Theses and Dissertations