subject:( Luciferase assay)

1. 五條堀,孝廣. Signaling pathways of electroliticaly-generated acid functional water in epithelial cells : 上皮細胞における電解酸性機能水のシグナル伝達経路.

Degree: 博士（歯学）, 2015, Nihon University / 日本大学

URL: http://repository.nihon-u.ac.jp/xmlui/handle/11263/554 ; http://dx.doi.org/10.15006/32665A4948

Subjects/Keywords: 電解酸性機能水; electrolyticaly-generated acid functional water; 2 本鎖 RNA; double-stranded RNA; ルシフェラーゼアッセイ; luciferase assay; hBD2; human β-defensin 2; IL-8; interleukin 8

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

五條堀,孝廣. (2015). Signaling pathways of electroliticaly-generated acid functional water in epithelial cells : 上皮細胞における電解酸性機能水のシグナル伝達経路. (Thesis). Nihon University / 日本大学. Retrieved from http://repository.nihon-u.ac.jp/xmlui/handle/11263/554 ; http://dx.doi.org/10.15006/32665A4948

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

五條堀,孝廣. “Signaling pathways of electroliticaly-generated acid functional water in epithelial cells : 上皮細胞における電解酸性機能水のシグナル伝達経路.” 2015. Thesis, Nihon University / 日本大学. Accessed December 13, 2019. http://repository.nihon-u.ac.jp/xmlui/handle/11263/554 ; http://dx.doi.org/10.15006/32665A4948.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

五條堀,孝廣. “Signaling pathways of electroliticaly-generated acid functional water in epithelial cells : 上皮細胞における電解酸性機能水のシグナル伝達経路.” 2015. Web. 13 Dec 2019.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

五條堀,孝廣. Signaling pathways of electroliticaly-generated acid functional water in epithelial cells : 上皮細胞における電解酸性機能水のシグナル伝達経路. [Internet] [Thesis]. Nihon University / 日本大学; 2015. [cited 2019 Dec 13]. Available from: http://repository.nihon-u.ac.jp/xmlui/handle/11263/554 ; http://dx.doi.org/10.15006/32665A4948.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

五條堀,孝廣. Signaling pathways of electroliticaly-generated acid functional water in epithelial cells : 上皮細胞における電解酸性機能水のシグナル伝達経路. [Thesis]. Nihon University / 日本大学; 2015. Available from: http://repository.nihon-u.ac.jp/xmlui/handle/11263/554 ; http://dx.doi.org/10.15006/32665A4948

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

2. Aqib Raza Khan, Mo. Studies on the Chemical Components of Marine-Derived Fungus, Aspergillus terreus and Their Biological Activities.

Degree: Master, Marine Biotechnology and Resources, 2016, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0801116-154952

► Abstract Marine microbes are regarded as one of the worthiest treasures for bioactive chemical Scaffolds. To search for anti-microbial secondary metabolites from marine environment, we… (more)

▼ Abstract Marine microbes are regarded as one of the worthiest treasures for bioactive chemical Scaffolds. To search for anti-microbial secondary metabolites from marine environment, we isolated a marine-derived fungus Aspergillus terreus (MB14-HBr) from the sponge Haliclona species, Collected from Dongsha Atoll, Taiwan in May 2014. The isolated fungus was further cultured in laboratory and fungal metabolites were extracted by ethyl acetate (EtOAc). Preliminary anti-microbial assay indicated that EtOAc extract of MB14-HBr could inhibit the bacterial pathogen Acinetobacter baumannii and the fungal one Candida albicans, respectively. Besides interestingly, we also discovered that the crude EtOAc extract, fraction as well as pure compounds including dihydrogeodin (A-01), butryolactone II (A-02), Butryolactone I (A-06), Terrelumamide A (A-11) and Methyl 3,4,5-trimethoxy-2-(2-nicotinamido)benzamido)benzoate (A-15) exhibited siderophore-like (iron-chelating) effects in Chrome-azurol S (CAS) assay. Due to natural iron clutching properties of sidherophores, they have impending applications in cancer chemotherapy. Acinetobacter baumannii is an opportunistic human pathogen which is easy mutant to be several clinical antibiotics and very famous due to cause of nosocomial infection in human. Moreover, three compounds, butryolactone II (A-02), Butryolactone I (A-06) and methyl 3, 5 dichloro-asterric acid (A-03) showed activity in luciferase assay, suggested that these components might block Transforming Growth Factor (TGF)-Î²-induced transcriptional responses. The objective of this study is to isolate the bioactive components from the strain Aspergillus terreus. In order to isolate bioactive components, the EtOAc extract was subjected to Sephadex LH-20 column chromatography and reversed phase high performance liquid chromatography. As a result, a total of fifteen known compounds have been isolated from this fungal extract, including dihydrogeodin (A-01) butryolactoneII (A-02), methyl 3,5 dichloro-asterrric acid (A-03), 3R-methyl-6-hydroxy-8-methoxy isocoumarin (A-04), terretonin C (A-05), butryolactone I (A-06), geodin hydrate (A-07), 6,7-dimethoxy-4-hydroxymellein (A-08), butryolactone V (A-09), asterric acid (A-10), terrein (A-12) asterrelenin (A-13), terretonin (A-14) and methyl 3,4,5-trimethoxy-2-(2-nicotinamido)benzamido)benzoate (A-15). Moreover, we isolated one are peptide named terrelumamide-A (A-11). The structures of these isolates were elucidated on the basis of 1D and 2D NMR, Mass spectrometry, as well as literature data. Key words: Aspegillus terreus, Anti-microbial activity, Acinetobacter baumannii, luciferase assay activity, MTS cell viability effect, Iron chelating effects. Advisors/Committee Members: Jyh-Horng Sheu (chair), Yu-Liang Yang (chair), Chih-Chuang Liaw (committee member), Jyh-Horng Sheu (chair).

Subjects/Keywords: luciferase assay activity; Acinetobacter baumannii; Anti-microbial activity; Aspegillus terreus; MTS cell viability effect; Iron chelating effects

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APA (6^th Edition):

Aqib Raza Khan, M. (2016). Studies on the Chemical Components of Marine-Derived Fungus, Aspergillus terreus and Their Biological Activities. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0801116-154952

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Aqib Raza Khan, Mo. “Studies on the Chemical Components of Marine-Derived Fungus, Aspergillus terreus and Their Biological Activities.” 2016. Thesis, NSYSU. Accessed December 13, 2019. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0801116-154952.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Aqib Raza Khan, Mo. “Studies on the Chemical Components of Marine-Derived Fungus, Aspergillus terreus and Their Biological Activities.” 2016. Web. 13 Dec 2019.

Vancouver:

Aqib Raza Khan M. Studies on the Chemical Components of Marine-Derived Fungus, Aspergillus terreus and Their Biological Activities. [Internet] [Thesis]. NSYSU; 2016. [cited 2019 Dec 13]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0801116-154952.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Aqib Raza Khan M. Studies on the Chemical Components of Marine-Derived Fungus, Aspergillus terreus and Their Biological Activities. [Thesis]. NSYSU; 2016. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0801116-154952

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rhode Island College

3. Bock, Timothy. Homology Modeling of the Squalus Acanthias AHR1 To Locate Structural Determinants of Function.

Degree: MA, 2019, Rhode Island College

URL: https://digitalcommons.ric.edu/etd/307

► The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor that mediates a toxic response to many environmental contaminants. Cartilaginous fishes, due to gene… (more)

Subjects/Keywords:

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bock, T. (2019). Homology Modeling of the Squalus Acanthias AHR1 To Locate Structural Determinants of Function. (Masters Thesis). Rhode Island College. Retrieved from https://digitalcommons.ric.edu/etd/307

Chicago Manual of Style (16^th Edition):

Bock, Timothy. “Homology Modeling of the Squalus Acanthias AHR1 To Locate Structural Determinants of Function.” 2019. Masters Thesis, Rhode Island College. Accessed December 13, 2019. https://digitalcommons.ric.edu/etd/307.

MLA Handbook (7^th Edition):

Bock, Timothy. “Homology Modeling of the Squalus Acanthias AHR1 To Locate Structural Determinants of Function.” 2019. Web. 13 Dec 2019.

Vancouver:

Bock T. Homology Modeling of the Squalus Acanthias AHR1 To Locate Structural Determinants of Function. [Internet] [Masters thesis]. Rhode Island College; 2019. [cited 2019 Dec 13]. Available from: https://digitalcommons.ric.edu/etd/307.

Council of Science Editors:

Bock T. Homology Modeling of the Squalus Acanthias AHR1 To Locate Structural Determinants of Function. [Masters Thesis]. Rhode Island College; 2019. Available from: https://digitalcommons.ric.edu/etd/307

University of Newcastle

4. Carroll, Adam. Genomic characterisation of small RNA-mediated post-transcriptional gene regulation.

Degree: PhD, 2013, University of Newcastle

URL: http://hdl.handle.net/1959.13/1037796

►

Research Doctorate - Doctor of Philosophy (PhD)

microRNA (miRNA) are small non-coding RNA molecules that function to guide the miRNA-induced silencing complex (miRISC) to regions… (more)

▼

Research Doctorate - Doctor of Philosophy (PhD)

microRNA (miRNA) are small non-coding RNA molecules that function to guide the miRNA-induced silencing complex (miRISC) to regions of complementarity within mRNA transcripts, thereby mediating sequence-specific post-transcriptional gene silencing (PTGS). This form of gene regulation is vital to a variety of biological processes in animals, from fundamental developmental functions including cellular proliferation and differentiation, to more complex and specialised roles such as long-term potentiation and synapse-specific modifications in neurons. While the complementarity of miRNA-mRNA interactions enables bioinformatic algorithms to predict potential target genes, the capacity for miRNA to interact with their targets through elements of only partial complementarity renders high false discovery rates to these predictions. With the discovery of increased cortical miRNA expression in schizophrenia, biological evidence was therefore required to investigate the function of schizophrenia-associated miRNA including miR-181b, miR-107, and members of the miR-15 and miR-17 families. Functional genomic techniques were established with genome-wide expression analysis and miRNA reporter-gene assays to characterise the biology of these miRNA in a variety of cellular contexts. In vitro characterisation of miR-181b revealed target gene regulation through interactions at both conserved and non-conserved MREs, with primary and secondary effects of miRNA modulation affecting many genes enriched within neurodevelopmental pathways vital to synaptic plasticity, including a number of schizophrenia candidate genes. Surprisingly, comparisons of miR-181b and miR-107 function against target prediction frameworks revealed a subset of predicted targets displaying positive correlation with cognate miRNA expression. Context-specific functional pleiotropy was also observed in different cellular environments, and this was further explored through miR-17/20a in T lymphocyte activation, and through miR-16 in cell cycle progression. Ultimately, substantial evidence was obtained to establish biological plausibility for miR-181b dysregulation as a potential aetiological factor in the development of schizophrenia, though the emergent theme of context-specific miRNA function requires future methodological developments and experiments to consider this functional pleiotropy when exploring miRNA function in normal developmental and pathophysiological states.

Advisors/Committee Members: University of Newcastle. Faculty of Health & Medicine, School of Biomedical Sciences and Pharmacy.

Subjects/Keywords: miRNA; schizophrenia; thesis by publication; target identification; luciferase assay; miR-181b; miR-107; miR-17; miR-20a; miR-16; expression

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Carroll, A. (2013). Genomic characterisation of small RNA-mediated post-transcriptional gene regulation. (Doctoral Dissertation). University of Newcastle. Retrieved from http://hdl.handle.net/1959.13/1037796

Chicago Manual of Style (16^th Edition):

Carroll, Adam. “Genomic characterisation of small RNA-mediated post-transcriptional gene regulation.” 2013. Doctoral Dissertation, University of Newcastle. Accessed December 13, 2019. http://hdl.handle.net/1959.13/1037796.

MLA Handbook (7^th Edition):

Carroll, Adam. “Genomic characterisation of small RNA-mediated post-transcriptional gene regulation.” 2013. Web. 13 Dec 2019.

Vancouver:

Carroll A. Genomic characterisation of small RNA-mediated post-transcriptional gene regulation. [Internet] [Doctoral dissertation]. University of Newcastle; 2013. [cited 2019 Dec 13]. Available from: http://hdl.handle.net/1959.13/1037796.

Council of Science Editors:

Carroll A. Genomic characterisation of small RNA-mediated post-transcriptional gene regulation. [Doctoral Dissertation]. University of Newcastle; 2013. Available from: http://hdl.handle.net/1959.13/1037796

Texas Medical Center

5. Chiang, Yun-Chen. Development of HIF-1α/HIF-1β heterodimerization inhibitors using a novel bioluminescence reporter assay system for in vitro high throughput screening and in vivo imaging.

Degree: PhD, 2013, Texas Medical Center

URL: http://digitalcommons.library.tmc.edu/utgsbs_dissertations/383

► Tumor growth often outpaces its vascularization, leading to development of a hypoxic tumor microenvironment. In response, an intracellular hypoxia survival pathway is initiated by… (more)

Subjects/Keywords: HIF-1α/β heterodimerization; HIF-1α inhibitor; cancer therapy; molecular imaging; high-content screnning; split luciferase complementation assay; Medical Biotechnology; Medical Molecular Biology; Medicine and Health Sciences; Pharmaceutics and Drug Design; Therapeutics

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APA (6^th Edition):

Chiang, Y. (2013). Development of HIF-1α/HIF-1β heterodimerization inhibitors using a novel bioluminescence reporter assay system for in vitro high throughput screening and in vivo imaging. (Doctoral Dissertation). Texas Medical Center. Retrieved from http://digitalcommons.library.tmc.edu/utgsbs_dissertations/383

Chicago Manual of Style (16^th Edition):

Chiang, Yun-Chen. “Development of HIF-1α/HIF-1β heterodimerization inhibitors using a novel bioluminescence reporter assay system for in vitro high throughput screening and in vivo imaging.” 2013. Doctoral Dissertation, Texas Medical Center. Accessed December 13, 2019. http://digitalcommons.library.tmc.edu/utgsbs_dissertations/383.

MLA Handbook (7^th Edition):

Chiang, Yun-Chen. “Development of HIF-1α/HIF-1β heterodimerization inhibitors using a novel bioluminescence reporter assay system for in vitro high throughput screening and in vivo imaging.” 2013. Web. 13 Dec 2019.

Vancouver:

Chiang Y. Development of HIF-1α/HIF-1β heterodimerization inhibitors using a novel bioluminescence reporter assay system for in vitro high throughput screening and in vivo imaging. [Internet] [Doctoral dissertation]. Texas Medical Center; 2013. [cited 2019 Dec 13]. Available from: http://digitalcommons.library.tmc.edu/utgsbs_dissertations/383.

Council of Science Editors:

Chiang Y. Development of HIF-1α/HIF-1β heterodimerization inhibitors using a novel bioluminescence reporter assay system for in vitro high throughput screening and in vivo imaging. [Doctoral Dissertation]. Texas Medical Center; 2013. Available from: http://digitalcommons.library.tmc.edu/utgsbs_dissertations/383

Louisiana State University

6. Dale, Renee. Mathematical Model of the Split Firefly Luciferase Assay.

Degree: MS, 2015, Louisiana State University

URL: etd-07022015-161605 ; https://digitalcommons.lsu.edu/gradschool_theses/855

► The firefly luciferase complementation assay is widely used as a bioluminescent reporter technology to detect protein-protein interactions in vitro and in vivo. Firefly luciferase oxidates… (more)

Subjects/Keywords: firefly luciferase; complementation assay; bioluminesence; reporter assay; mathematical model

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APA (6^th Edition):

Dale, R. (2015). Mathematical Model of the Split Firefly Luciferase Assay. (Masters Thesis). Louisiana State University. Retrieved from etd-07022015-161605 ; https://digitalcommons.lsu.edu/gradschool_theses/855

Chicago Manual of Style (16^th Edition):

Dale, Renee. “Mathematical Model of the Split Firefly Luciferase Assay.” 2015. Masters Thesis, Louisiana State University. Accessed December 13, 2019. etd-07022015-161605 ; https://digitalcommons.lsu.edu/gradschool_theses/855.

MLA Handbook (7^th Edition):

Dale, Renee. “Mathematical Model of the Split Firefly Luciferase Assay.” 2015. Web. 13 Dec 2019.

Vancouver:

Dale R. Mathematical Model of the Split Firefly Luciferase Assay. [Internet] [Masters thesis]. Louisiana State University; 2015. [cited 2019 Dec 13]. Available from: etd-07022015-161605 ; https://digitalcommons.lsu.edu/gradschool_theses/855.

Council of Science Editors:

Dale R. Mathematical Model of the Split Firefly Luciferase Assay. [Masters Thesis]. Louisiana State University; 2015. Available from: etd-07022015-161605 ; https://digitalcommons.lsu.edu/gradschool_theses/855

Duquesne University

7. Das, Ranajit. Molecular Evolution of Hominoid Primates: Phylogeny and Regulation.

Degree: PhD, Biological Sciences, 2014, Duquesne University

URL: https://dsc.duq.edu/etd/461

► The complete mtDNA of one eastern gorilla was sequenced to provide the most accurate date for the mitochondrial divergence of gorillas. The most recent common… (more)

▼ The complete mtDNA of one eastern gorilla was sequenced to provide the most accurate date for the mitochondrial divergence of gorillas. The most recent common ancestor of eastern lowland and western lowland gorillas existed about 1.9 million years ago, slightly more recent than that of chimpanzee and bonobo. This study also depicts that the eastern and western gorillas show species level genetic divergence. Hominoid mating systems differ tremendously. The level of sperm competition varies according to the mating system, which presumably imposes unique selective pressures on the seminal proteins of each species. Cartilage acidic protein 1 (CRTAC1) was identified in our lab as the protein with the largest difference in abundance between human and chimpanzee, being found at 142-fold higher in chimpanzee. The coding region of CRTAC1 is extremely conserved with signature of strong purifying selection. Paradoxically, CRTAC1 `promoter' from human drives transcription significantly greater than chimpanzee, with or without androgen stimulation. Analyzing H3K27Ac data, a ~2.2kb region was identified as a possible additional cis-regulatory element. The cis-regulatory region behaved like a silencer and aided in strong transcriptional repression in humans. Although its underlying basis remains elusive, it can be speculated that the differential expression of CRTAC1 between human and chimpanzee seminal plasma results from tissue specific over/under expression of this gene. The unique gains and losses of miRNAs within hominoids have remained understudied. The overall goal of this project was to identify the uniquely gained and lost miRNAs and their targets within hominoids. I found 14 miRNAs uniquely gained in humans. Maximum uniquely gained and lost miRNAs were found to be brain specific. The targets of uniquely gained miRNAs in human are also associated with brain-associated functions. Older miRNAs were found to be more conserved compared to the newer miRNAs gained <15 Mya. Advisors/Committee Members: Michael I Jensen-Seaman, Brady Porter, David Lampe, Nathan Clark.

Subjects/Keywords: Eastern Lowland Gorilla; In vitro Luciferase Assay; Promoter-Silencer Interaction; Seminal plasma proteins; TMRCA; Unique hominoid miRNAs

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Das, R. (2014). Molecular Evolution of Hominoid Primates: Phylogeny and Regulation. (Doctoral Dissertation). Duquesne University. Retrieved from https://dsc.duq.edu/etd/461

Chicago Manual of Style (16^th Edition):

Das, Ranajit. “Molecular Evolution of Hominoid Primates: Phylogeny and Regulation.” 2014. Doctoral Dissertation, Duquesne University. Accessed December 13, 2019. https://dsc.duq.edu/etd/461.

MLA Handbook (7^th Edition):

Das, Ranajit. “Molecular Evolution of Hominoid Primates: Phylogeny and Regulation.” 2014. Web. 13 Dec 2019.

Vancouver:

Das R. Molecular Evolution of Hominoid Primates: Phylogeny and Regulation. [Internet] [Doctoral dissertation]. Duquesne University; 2014. [cited 2019 Dec 13]. Available from: https://dsc.duq.edu/etd/461.

Council of Science Editors:

Das R. Molecular Evolution of Hominoid Primates: Phylogeny and Regulation. [Doctoral Dissertation]. Duquesne University; 2014. Available from: https://dsc.duq.edu/etd/461

8. Fath, Puria Motamed. Construction of a Copper Bioreporter Screening, characterization and genetic improvement of copper-sensitive bacteria.

Degree: Engineering, 2010, University of Borås

URL: http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-19682

► In the nature, lots of organism apply different kinds of lights such as flourscence or luminoscence for some purposes such as defence or hunting.… (more)

Subjects/Keywords: copper bioreporter; luciferase assay; cop operon; pgl3; e. coli bl-21; Engineering and Technology; Teknik och teknologier

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APA (6^th Edition):

Fath, P. M. (2010). Construction of a Copper Bioreporter Screening, characterization and genetic improvement of copper-sensitive bacteria. (Thesis). University of Borås. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-19682

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fath, Puria Motamed. “Construction of a Copper Bioreporter Screening, characterization and genetic improvement of copper-sensitive bacteria.” 2010. Thesis, University of Borås. Accessed December 13, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-19682.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fath, Puria Motamed. “Construction of a Copper Bioreporter Screening, characterization and genetic improvement of copper-sensitive bacteria.” 2010. Web. 13 Dec 2019.

Vancouver:

Fath PM. Construction of a Copper Bioreporter Screening, characterization and genetic improvement of copper-sensitive bacteria. [Internet] [Thesis]. University of Borås; 2010. [cited 2019 Dec 13]. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-19682.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fath PM. Construction of a Copper Bioreporter Screening, characterization and genetic improvement of copper-sensitive bacteria. [Thesis]. University of Borås; 2010. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-19682

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université de Montréal

9. Forget, Amélie. La voie de régulation de la traduction de l’ARNm ASH1 : une concertation entre Khd1, Puf6 et Loc1 .

Degree: 2014, Université de Montréal

URL: http://hdl.handle.net/1866/11329

► La localisation des ARNm par transport dirigé joue un rôle dans le développement, la motilité cellulaire, la plasticité synaptique et la division cellulaire asymétrique. Chez… (more)

▼ La localisation des ARNm par transport dirigé joue un rôle dans le développement, la motilité cellulaire, la plasticité synaptique et la division cellulaire asymétrique. Chez la levure Saccharomyces cerevisiæ, la localisation d’ARNm est un phénomène dont les mécanismes de régulation sont conservés auprès de nombreux autres organismes. Lors de la division de la levure, plus d’une trentaine de transcrits sont localisés par transport actif à l’extrémité du bourgeon de la cellule-fille. Parmi ceux-ci, l’ARNm ASH1 est le mieux caractérisé et constitue le modèle utilisé dans cette étude. Pour exercer sa fonction, la protéine Ash1 doit être produite uniquement après la localisation de l’ARNm ASH1. Pour ce faire, les mécanismes de régulation de la traduction de l’ARNm ASH1 empêchent son expression durant le transport. Ce projet de recherche vise à étudier les mécanismes de régulation de la traduction de l’ARNm ASH1 par les répresseurs traductionnels connus, soit Khd1, Puf6 et Loc1. Les études antérieures se sont penchées sur ces facteurs de manière individuelle. Cependant, dans cette étude, nous avons exploré la présence d’une collaboration entre ceux-ci. Ainsi, nous avons voulu déterminer si les répresseurs traductionnels peuvent être intégrés en une seule voie de régulation de la traduction de l’ARNm ASH1. De plus, nous avons cherché à identifier le mécanisme de recrutement des répresseurs traductionnels sur l’ARNm ASH1, qui correspond au point initial des voies de régulations de l’ARNm ASH1. Nos résultats montrent que les répresseurs traductionnels de l’ARNm ASH1, soit Khd1 et Puf6, font partie d’une même voie de régulation de la traduction. Le rôle du facteur nucléaire Loc1 dans la voie de régulation de la traduction, quant à elle, a été examinée à partir d’expériences permettant l’étude du mécanisme de recrutement des répresseurs traductionnels dans le noyau. Ainsi, nos travaux montrent que Puf6 et Loc1 sont associés de manière ARN-dépendant avec la machinerie de transcription, notamment au facteur d’élongation de la transcription Spt4-Spt5/DSIF. Par ailleurs, notre laboratoire a précédemment montré que la localisation nucléaire de la protéine de liaison à l’ARN She2 est essentielle au recrutement des facteurs Loc1 et Puf6 sur l’ARNm ASH1. Des expériences d’immunoprécipitation de la chromatine (ChIP) supportent l’hypothèse que le recrutement de Loc1 est essentiel à celui de Puf6, qui s’effectue ultérieurement. Ainsi, à partir des résultats de cette étude et des résultats publiés précédemment dans notre laboratoire, nous avons élaboré un modèle de recrutement coordonné des facteurs She2, Loc1 et Puf6 sur l’ARNm ASH1 naissant. De manière générale, cette étude a permis d’établir la présence d’une seule voie de régulation de la traduction de l’ARNm ASH1 et une meilleure connaissance du recrutement des facteurs de répression traductionnelle sur celui-ci. Advisors/Committee Members: Chartrand, Pascal (advisor).

Subjects/Keywords: localisation d’ARNm dirigé; ARNm ASH1; Saccharomyces cerevisiæ; division cellulaire asymétrique; mécanismes de régulation de la traduction; répresseurs traductionnels; Khd1; Puf6; Loc1; She2; Spt4-Spt5/DSIF; co-IP; ChIP; luciferase assay; mRNA localization; ASH1 mRNA; yeast; asymmetric cellular division; translation regulation pathway

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APA (6^th Edition):

Forget, A. (2014). La voie de régulation de la traduction de l’ARNm ASH1 : une concertation entre Khd1, Puf6 et Loc1 . (Thesis). Université de Montréal. Retrieved from http://hdl.handle.net/1866/11329

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Forget, Amélie. “La voie de régulation de la traduction de l’ARNm ASH1 : une concertation entre Khd1, Puf6 et Loc1 .” 2014. Thesis, Université de Montréal. Accessed December 13, 2019. http://hdl.handle.net/1866/11329.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Forget, Amélie. “La voie de régulation de la traduction de l’ARNm ASH1 : une concertation entre Khd1, Puf6 et Loc1 .” 2014. Web. 13 Dec 2019.

Vancouver:

Forget A. La voie de régulation de la traduction de l’ARNm ASH1 : une concertation entre Khd1, Puf6 et Loc1 . [Internet] [Thesis]. Université de Montréal; 2014. [cited 2019 Dec 13]. Available from: http://hdl.handle.net/1866/11329.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Forget A. La voie de régulation de la traduction de l’ARNm ASH1 : une concertation entre Khd1, Puf6 et Loc1 . [Thesis]. Université de Montréal; 2014. Available from: http://hdl.handle.net/1866/11329

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Helsinki

10. Gao, Song. Detection of protein- protein interactions in planta by BiFC and split luciferase assays.

Degree: Department of Agricultural Sciences; Helsingfors universitet, Agrikultur- och forstvetenskapliga fakulteten, Institutionen för lantsbruksvetenskaper, 2010, University of Helsinki

URL: http://hdl.handle.net/10138/18107

► Molecular biology has created a new pathway for plant breeding in cut flower industry. It focuses on studying flower gene functions and provides a more… (more)

▼ Molecular biology has created a new pathway for plant breeding in cut flower industry. It focuses on studying flower gene functions and provides a more direct and effective way of breeding new flower cultivars using genetic transformation. Besides flower color, disease resistance, quality and vase life, modification of flower architecture is an important target for flower breeding. Previous studies have showed that various transcription factors encoded by the corresponding genes are involved regulating flower development and flower architecture. The most studied are MADS domain and TCP domain transcription factors. For targeted breeding, it is crucial to study the functions of the corresponding genes in detail. For both MADS and TCP domain proteins, previous studies have indicated that protein-protein interactions are important for their function. GhCYC1, GhCYC2, GhCYC3 and GhCYC4, isolated from gerbera (Gerbera hybrida), are CYCLOIDEA –like genes affecting inflorescence development. The protein-protein interactions among these four genes have previously been studied by yeast two-hybrid system. The aim of this thesis was to verify the interactions in living plant cells, using both BiFC and split luciferase assays. Protoplast electroporation and agroinfiltration were used to introduce the genes in planta. The results from the two assays were compared in order to find an effective in planta method for detecting protein-protein interactions. The experiment also provided information about DNA transformation efficiency using protoplast electroporation and agroinfiltration. The results of the split luciferase assay showed that GhCYC1+GhCYC4, GhCYC3+GhCYC4 as well as GhCYC4+GhCYC4 interacted quite strongly in plant cells while GhCYC1+GhCYC1, GhCYC2+GhCYC2 as well as GhCYC4+GhCYC2 had almost no interactions. The interactions between GhCYC3+GhCYC4, and GhCYC4+ GhCYC4 were also shown in yeast two-hybrid, but the other results were different. According to the BiFC assay, no signals of interactions were detected from GhCYC2+GhCYC2, while strong signals were observed from GhCYC2+GhCYC3, and weak signals were seen from GhCYC2+GhCYC4. The interactions between GhCYC2+GhCYC3, GhCYC2+GhCYC4 were also observed in yeast two-hybrid, but the other results were unconfirmed. Large standard deviations were observed in the split luciferase assay and thereby reliable conclusions cannot be drawn from it. However, BiFC turned out to be a better method to detect the protein-protein interactions in planta and clear signals from interactions could be observed. Comparison of the transformation methods indicated that agroinfiltration is a better way of introducing DNA into plant cells than protoplast electroporation. For further study, BiFC assay still needs to be repeated to confirm the efficiency of this assay, and factors affecting the transformation efficiency in protoplast electroporation need to be optimized in the future studies.

Subjects/Keywords: protein- protein interactions; BiFC; split luciferase assay; electroporation; agroinfiltration; Växtproduktions biologi (trädgårdsvetenskap); Plant Production Biology (Horticulture); Kasvintuotannon biologia (puutarhatiede); protein- protein interactions; BiFC; split luciferase assay; electroporation; agroinfiltration

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gao, S. (2010). Detection of protein- protein interactions in planta by BiFC and split luciferase assays. (Masters Thesis). University of Helsinki. Retrieved from http://hdl.handle.net/10138/18107

Chicago Manual of Style (16^th Edition):

Gao, Song. “Detection of protein- protein interactions in planta by BiFC and split luciferase assays.” 2010. Masters Thesis, University of Helsinki. Accessed December 13, 2019. http://hdl.handle.net/10138/18107.

MLA Handbook (7^th Edition):

Gao, Song. “Detection of protein- protein interactions in planta by BiFC and split luciferase assays.” 2010. Web. 13 Dec 2019.

Vancouver:

Gao S. Detection of protein- protein interactions in planta by BiFC and split luciferase assays. [Internet] [Masters thesis]. University of Helsinki; 2010. [cited 2019 Dec 13]. Available from: http://hdl.handle.net/10138/18107.

Council of Science Editors:

Gao S. Detection of protein- protein interactions in planta by BiFC and split luciferase assays. [Masters Thesis]. University of Helsinki; 2010. Available from: http://hdl.handle.net/10138/18107

Universitat Pompeu Fabra

11. Guidi, Mònica. Micro RNA-Mediated regulation of the full-length and truncated isoforms of human neurotrophic tyrosine kinase receptor type 3 (NTRK 3).

Degree: Departament de Ciències Experimentals i de la Salut, 2009, Universitat Pompeu Fabra

URL: http://hdl.handle.net/10803/7114

► Las neurotrofinas y sus receptores constituyen una familia de factores cruciales para el desarrollo del sistema nervioso. La neurotrofina 3 ejerce su función principalmente a… (more)

Subjects/Keywords: RISC complex; retinoic acid; renilla luciferase; real-time quantitative PCR; PTBP1; pri-miRNA; pre-miRNA; post-transcriptional regulation; piRNA; PicTar; pGL4.13; PCR; P-body; p75NTR; overexpression; NTRK3; NTRK2; NTRK1; non-coding RNAs; NGF; neurotrophin; neurotrophic signaling; neuronal differentiation; neurodevelopment; neuroblastoma; nervous system; mRNA; miRNA target prediction; miRNA profiling; miRNA mimic; miRNA microarray; miRNA inhibitor; miRanda; microRNA; microarray; luciferase assay; Ingenuity Pathway Analysis (IPA); heLa cells; gene silencing; gene regulation; full-length isoform (FL-NTRK3); firefly luciferase; ERK; disease; dicer; cancer development; BDNF; argonaute; alternative splicing; 3'UTR; RNAhybrid; RT-PCR; SH-SY5Y cells; siRNA; standard error; synaptic plasticity; target validation; TargetScan; transfection; translational repression; TrkA; TrkB; TrkC; truncated isoform (TR-NTRK3); tyrosine kinase (TK); western blot; 575; 61

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Guidi, M. (2009). Micro RNA-Mediated regulation of the full-length and truncated isoforms of human neurotrophic tyrosine kinase receptor type 3 (NTRK 3). (Thesis). Universitat Pompeu Fabra. Retrieved from http://hdl.handle.net/10803/7114

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Guidi, Mònica. “Micro RNA-Mediated regulation of the full-length and truncated isoforms of human neurotrophic tyrosine kinase receptor type 3 (NTRK 3).” 2009. Thesis, Universitat Pompeu Fabra. Accessed December 13, 2019. http://hdl.handle.net/10803/7114.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Guidi, Mònica. “Micro RNA-Mediated regulation of the full-length and truncated isoforms of human neurotrophic tyrosine kinase receptor type 3 (NTRK 3).” 2009. Web. 13 Dec 2019.

Vancouver:

Guidi M. Micro RNA-Mediated regulation of the full-length and truncated isoforms of human neurotrophic tyrosine kinase receptor type 3 (NTRK 3). [Internet] [Thesis]. Universitat Pompeu Fabra; 2009. [cited 2019 Dec 13]. Available from: http://hdl.handle.net/10803/7114.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Guidi M. Micro RNA-Mediated regulation of the full-length and truncated isoforms of human neurotrophic tyrosine kinase receptor type 3 (NTRK 3). [Thesis]. Universitat Pompeu Fabra; 2009. Available from: http://hdl.handle.net/10803/7114

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Uppsala University

12. Handin, Niklas. Identification of new regulatory mechanisms that determine coagulation FXI plasma concentration.

Degree: Biology Education Centre, 2015, Uppsala University

URL: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-261267

► FXI is a protein in the coagulation cascade in humans proven to be involved in the propagation and stabilization of developing thrombi in previous… (more)

Subjects/Keywords: FXI; F11; blood plasma; coagulation; GWAS; Meta-analysis; pathway analysis; eQTL; miRNA; luciferase reporter assay

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Handin, N. (2015). Identification of new regulatory mechanisms that determine coagulation FXI plasma concentration. (Thesis). Uppsala University. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-261267

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Handin, Niklas. “Identification of new regulatory mechanisms that determine coagulation FXI plasma concentration.” 2015. Thesis, Uppsala University. Accessed December 13, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-261267.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Handin, Niklas. “Identification of new regulatory mechanisms that determine coagulation FXI plasma concentration.” 2015. Web. 13 Dec 2019.

Vancouver:

Handin N. Identification of new regulatory mechanisms that determine coagulation FXI plasma concentration. [Internet] [Thesis]. Uppsala University; 2015. [cited 2019 Dec 13]. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-261267.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Handin N. Identification of new regulatory mechanisms that determine coagulation FXI plasma concentration. [Thesis]. Uppsala University; 2015. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-261267

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université de Montréal

13. Hannou, Lydia. La régulation transcriptionnelle de Neuroligine-1 par les facteurs de transcription de l’horloge .

Degree: 2018, Université de Montréal

URL: http://hdl.handle.net/1866/20787

Subjects/Keywords: Neuroligine-1; Transcription; Protéines de l’horloge; Essai luciférase; Expression génique; Souris; Neuroligin-1; Transcription; Clock proteins; Luciferase assay; Gene expression; Mice

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APA (6^th Edition):

Hannou, L. (2018). La régulation transcriptionnelle de Neuroligine-1 par les facteurs de transcription de l’horloge . (Thesis). Université de Montréal. Retrieved from http://hdl.handle.net/1866/20787

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hannou, Lydia. “La régulation transcriptionnelle de Neuroligine-1 par les facteurs de transcription de l’horloge .” 2018. Thesis, Université de Montréal. Accessed December 13, 2019. http://hdl.handle.net/1866/20787.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hannou, Lydia. “La régulation transcriptionnelle de Neuroligine-1 par les facteurs de transcription de l’horloge .” 2018. Web. 13 Dec 2019.

Vancouver:

Hannou L. La régulation transcriptionnelle de Neuroligine-1 par les facteurs de transcription de l’horloge . [Internet] [Thesis]. Université de Montréal; 2018. [cited 2019 Dec 13]. Available from: http://hdl.handle.net/1866/20787.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hannou L. La régulation transcriptionnelle de Neuroligine-1 par les facteurs de transcription de l’horloge . [Thesis]. Université de Montréal; 2018. Available from: http://hdl.handle.net/1866/20787

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

14. Hogel, Matthew. INVESTIGATING THE MECHANISM OF PROMOTER-SPECIFIC N-TERMINAL MUTANT HUNTINGTIN-MEDIATED TRANSCRIPTIONAL DYSREGULATION.

Degree: PhD, Department of Pharmacology with Neuroscience, 2012, Dalhousie University

URL: http://hdl.handle.net/10222/15723

► Huntington’s disease (HD) is a neurodegenerative disorder caused by the inheritance of one mutant copy of the huntingtin gene. Mutant huntingtin protein (mHtt) contains an… (more)

▼ Huntington’s disease (HD) is a neurodegenerative disorder caused by the inheritance of one mutant copy of the huntingtin gene. Mutant huntingtin protein (mHtt) contains an expanded polyglutamine repeat region near the N-terminus. Cleavage of mHtt releases an N-terminal fragment (N-mHtt) which translocates, and accumulates in the nucleus. Nuclear accumulation of N-mHtt has been directly associated with cellular toxicity. Decreased transcription is among the earliest detected changes that occur in the brains of HD patients and is consistently observed in all animal and cellular models of HD. Transcriptional dysregulation may trigger many of the perturbations that occur later in disease progression and an understanding of the effects of mHtt may lead to strategies to slow the progression of the disease. Current models of N-mHtt-mediated transcriptional dysregulation suggest that abnormal interactions between N-mHtt and transcription factors impair the ability of these transcription factors to associate at N-mHtt-affected promoters and properly regulate gene expression. We tested various aspects of these models using two N-mHtt-affected promoters in in vitro transcription assays and in two cell models of HD using techniques including overexpression of known N-mHtt-interacting transcription factors, chromatin immunoprecipitation, promoter deletion and mutation analyses and in vitro promoter binding assays. Based on our results and those in the literature, we proposed a new model of N-mHtt-mediated transcriptional dysregulation centered on the presence of N-mHtt at affected promoters. We concluded that simultaneous interaction of N-mHtt with multiple binding partners within the transcriptional machinery would explain the gene-specificity of N-mHtt-mediated transcriptional dysregulation, as well as the observation that some genes are affected early in disease progression while others are affected later. Our model explains why alleviating N-mHtt-mediated transcriptional dysregulation through overexpression of N-mHtt-interacting proteins has proven to be difficult and suggests that the most realistic strategy for restoring gene expression across the spectrum of N-mHtt affected genes is by reducing the amount of soluble nuclear N-mHtt. Advisors/Committee Members: Dr. Simonetta Sipione (external-examiner), Dr. Jana Sawynok (graduate-coordinator), Dr. Elizabeth Cowley (thesis-reader), Dr. George Robertson (thesis-reader), Dr. Eileen Denovan-Wright (thesis-supervisor), Not Applicable (ethics-approval), Not Applicable (manuscripts), Not Applicable (copyright-release).

Subjects/Keywords: Huntington's; Transcription; Repression; Huntingtin; Polyglutamine; Transcriptional Dysregulation; in vitro transcription; N548; ST14A; Dual-luciferase assay; Chromatin Immunoprecipitation; Promoter Deletion; Linker Scanning Mutagenesis; Quantitative PCR; Promoter binding assay; TBP; RAP30

…3.4 Diagram of the luciferase reporter assay… …of the luciferase assay… …3.9 CMV-driven luciferase activity was decreased in the presence of N-mHtt ... 111 3.10… …The relationship between luciferase activity and luc+ mRNA copy number was similar in N548wt… …and N548hd cells but not in ST14A cells .................. 112 3.11 Luciferase activity…

10 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hogel, M. (2012). INVESTIGATING THE MECHANISM OF PROMOTER-SPECIFIC N-TERMINAL MUTANT HUNTINGTIN-MEDIATED TRANSCRIPTIONAL DYSREGULATION. (Doctoral Dissertation). Dalhousie University. Retrieved from http://hdl.handle.net/10222/15723

Chicago Manual of Style (16^th Edition):

Hogel, Matthew. “INVESTIGATING THE MECHANISM OF PROMOTER-SPECIFIC N-TERMINAL MUTANT HUNTINGTIN-MEDIATED TRANSCRIPTIONAL DYSREGULATION.” 2012. Doctoral Dissertation, Dalhousie University. Accessed December 13, 2019. http://hdl.handle.net/10222/15723.

MLA Handbook (7^th Edition):

Hogel, Matthew. “INVESTIGATING THE MECHANISM OF PROMOTER-SPECIFIC N-TERMINAL MUTANT HUNTINGTIN-MEDIATED TRANSCRIPTIONAL DYSREGULATION.” 2012. Web. 13 Dec 2019.

Vancouver:

Hogel M. INVESTIGATING THE MECHANISM OF PROMOTER-SPECIFIC N-TERMINAL MUTANT HUNTINGTIN-MEDIATED TRANSCRIPTIONAL DYSREGULATION. [Internet] [Doctoral dissertation]. Dalhousie University; 2012. [cited 2019 Dec 13]. Available from: http://hdl.handle.net/10222/15723.

Council of Science Editors:

Hogel M. INVESTIGATING THE MECHANISM OF PROMOTER-SPECIFIC N-TERMINAL MUTANT HUNTINGTIN-MEDIATED TRANSCRIPTIONAL DYSREGULATION. [Doctoral Dissertation]. Dalhousie University; 2012. Available from: http://hdl.handle.net/10222/15723

15. Hsia, Hung-Ching. Identification of Targets and Pathways Controlled by the Chicken MicroRNAs miR-10a and miR-143.

Degree: MS, Animal Science, 2009, North Carolina State University

URL: http://www.lib.ncsu.edu/resolver/1840.16/942

► MicroRNAs are small non-coding RNAs that regulate gene expression at the posttranscriptional level. The importance of microRNAs in development, tumorogenesis, immune system function, and infectious… (more)

▼ MicroRNAs are small non-coding RNAs that regulate gene expression at the posttranscriptional level. The importance of microRNAs in development, tumorogenesis, immune system function, and infectious diseases has gradually come to light. MicroRNAs are often expressed in a temporal and spatial manner and regulate specific gene sets to achieve phenotypic change. To understand the role of microRNAs in the chicken embryonic spleen, we have profiled microRNA expression at E15 and E20 chicken embryos. The objective of the current project is to identify the targets and pathways controlled by two microRNAs, miR-10a and miR-143. Target prediction was carried out by the algorithm miRanda. Eight predicted targets of each microRNA were subjected to validation using a reporter assay that incorporates synthetic or retroviral transduced microRNA. Validated targets for miR-143 known to have profound functions in apoptosis, T and B cell development and maturation, cancer, or adipocyte differentiation were identified with this approach; for miR-10a, validated targets involved in hematopoiesis, immune cells homing and migration and megakaryocyte differentiation were identified. Microarray analysis revealed that several targets not predicted by the algorithm were upregulated upon treatment with a specific microRNA inhibitor. This result suggests that both microRNAs may regulate genes that contribute to cancer formation. In addition, miR-10a may be involved in regulating the complement system, which is a crucial component of innate immunity; miR-143 may be involved in the PPAR/RXR pathway, which regulates glucose and lipid metabolism in cells. Taken together, our results suggest targets and pathways possibly regulated by miR-10a or miR-143. The inferred functions of the targets and pathways identified in our study are mostly consistent with those from previous studies, thereby providing insights and directions for future research into the mechanisms by which miR-10a and miR-143 exert their function in the developing chick embryo. Furthermore, the RCAS system used in this project also proved the usefulness of engineered retrovirus for transducing microRNA. Further development of this tool may aid microRNA research and further contribute to understanding of the role of microRNAs in vertebrate development. Advisors/Committee Members: Dr. H. -C. Sunny Liu, Committee Chair (advisor), Dr. Bob Petters, Committee Member (advisor), Dr. Chad Stahl, Committee Member (advisor).

Subjects/Keywords: spleen; chicken; microRNA; luciferase assay; microarray

…39 Luciferase Assay… …49 Luciferase Assay… …62 Luciferase Assay… …55 Figure 3.5 Luciferase Assay for miR-10a Target Validation… …56 Figure 3.6 Luciferase Assay for miR-143 Target Validation…

1 more image…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hsia, H. (2009). Identification of Targets and Pathways Controlled by the Chicken MicroRNAs miR-10a and miR-143. (Thesis). North Carolina State University. Retrieved from http://www.lib.ncsu.edu/resolver/1840.16/942

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hsia, Hung-Ching. “Identification of Targets and Pathways Controlled by the Chicken MicroRNAs miR-10a and miR-143.” 2009. Thesis, North Carolina State University. Accessed December 13, 2019. http://www.lib.ncsu.edu/resolver/1840.16/942.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hsia, Hung-Ching. “Identification of Targets and Pathways Controlled by the Chicken MicroRNAs miR-10a and miR-143.” 2009. Web. 13 Dec 2019.

Vancouver:

Hsia H. Identification of Targets and Pathways Controlled by the Chicken MicroRNAs miR-10a and miR-143. [Internet] [Thesis]. North Carolina State University; 2009. [cited 2019 Dec 13]. Available from: http://www.lib.ncsu.edu/resolver/1840.16/942.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hsia H. Identification of Targets and Pathways Controlled by the Chicken MicroRNAs miR-10a and miR-143. [Thesis]. North Carolina State University; 2009. Available from: http://www.lib.ncsu.edu/resolver/1840.16/942

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Kyoto University / 京都大学

16. Isobe, Masanori. Polymorphism within a neuronal activity-dependent enhancer of NgR1 is associated with corpus callosum morphology in humans : NgR1遺伝子の神経活動依存性エンハンサー領域の遺伝子多型はヒトの脳梁の形態に関連する.

Degree: 博士(医学), 2015, Kyoto University / 京都大学

URL: http://hdl.handle.net/2433/202673 ; http://dx.doi.org/10.14989/doctor.k19270

新制・課程博士

甲第19270号

医博第4034号

Subjects/Keywords: Nogo-66 receptor 1 (NgR1); Corpus callosum; Diffusion tensor imaging (DTI); Epigenomics; Luciferase assay; Neuronal activity dependent enhancer

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Isobe, M. (2015). Polymorphism within a neuronal activity-dependent enhancer of NgR1 is associated with corpus callosum morphology in humans : NgR1遺伝子の神経活動依存性エンハンサー領域の遺伝子多型はヒトの脳梁の形態に関連する. (Thesis). Kyoto University / 京都大学. Retrieved from http://hdl.handle.net/2433/202673 ; http://dx.doi.org/10.14989/doctor.k19270

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Isobe, Masanori. “Polymorphism within a neuronal activity-dependent enhancer of NgR1 is associated with corpus callosum morphology in humans : NgR1遺伝子の神経活動依存性エンハンサー領域の遺伝子多型はヒトの脳梁の形態に関連する.” 2015. Thesis, Kyoto University / 京都大学. Accessed December 13, 2019. http://hdl.handle.net/2433/202673 ; http://dx.doi.org/10.14989/doctor.k19270.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Isobe, Masanori. “Polymorphism within a neuronal activity-dependent enhancer of NgR1 is associated with corpus callosum morphology in humans : NgR1遺伝子の神経活動依存性エンハンサー領域の遺伝子多型はヒトの脳梁の形態に関連する.” 2015. Web. 13 Dec 2019.

Vancouver:

Isobe M. Polymorphism within a neuronal activity-dependent enhancer of NgR1 is associated with corpus callosum morphology in humans : NgR1遺伝子の神経活動依存性エンハンサー領域の遺伝子多型はヒトの脳梁の形態に関連する. [Internet] [Thesis]. Kyoto University / 京都大学; 2015. [cited 2019 Dec 13]. Available from: http://hdl.handle.net/2433/202673 ; http://dx.doi.org/10.14989/doctor.k19270.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Isobe M. Polymorphism within a neuronal activity-dependent enhancer of NgR1 is associated with corpus callosum morphology in humans : NgR1遺伝子の神経活動依存性エンハンサー領域の遺伝子多型はヒトの脳梁の形態に関連する. [Thesis]. Kyoto University / 京都大学; 2015. Available from: http://hdl.handle.net/2433/202673 ; http://dx.doi.org/10.14989/doctor.k19270

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

17. Isobe, Masanori. Polymorphism within a neuronal activity-dependent enhancer of NgR1 is associated with corpus callosum morphology in humans .

Degree: 2015, Kyoto University

URL: http://hdl.handle.net/2433/202673

Subjects/Keywords: Nogo-66 receptor 1 (NgR1); Corpus callosum; Diffusion tensor imaging (DTI); Epigenomics; Luciferase assay; Neuronal activity dependent enhancer

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Isobe, M. (2015). Polymorphism within a neuronal activity-dependent enhancer of NgR1 is associated with corpus callosum morphology in humans . (Thesis). Kyoto University. Retrieved from http://hdl.handle.net/2433/202673

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Isobe, Masanori. “Polymorphism within a neuronal activity-dependent enhancer of NgR1 is associated with corpus callosum morphology in humans .” 2015. Thesis, Kyoto University. Accessed December 13, 2019. http://hdl.handle.net/2433/202673.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Isobe, Masanori. “Polymorphism within a neuronal activity-dependent enhancer of NgR1 is associated with corpus callosum morphology in humans .” 2015. Web. 13 Dec 2019.

Vancouver:

Isobe M. Polymorphism within a neuronal activity-dependent enhancer of NgR1 is associated with corpus callosum morphology in humans . [Internet] [Thesis]. Kyoto University; 2015. [cited 2019 Dec 13]. Available from: http://hdl.handle.net/2433/202673.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Isobe M. Polymorphism within a neuronal activity-dependent enhancer of NgR1 is associated with corpus callosum morphology in humans . [Thesis]. Kyoto University; 2015. Available from: http://hdl.handle.net/2433/202673

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

18. Jahanshahi-Anbuhi, Sana. Development of Ready-to-Use Biosensors for Diagnostics and Biosensing.

Degree: DEng, 2014, McMaster University

URL: http://hdl.handle.net/11375/16501

► Ideally, every person in the world should have access to a safe and clean water supply; if not all sources of water are clean and… (more)

▼ Ideally, every person in the world should have access to a safe and clean water supply; if not all sources of water are clean and safe, at the very least, an effective method to detect water contamination should be readily available. An effective detection method should not only be sensitive, rapid, robust, and affordable, but, ideally, it should also be equipment-free and easy to transport and deliver to the end-users. The main goal of this project is to develop a variety of bits and pieces of bioassay systems, with a particular focus on paper-based bioactive devices in order to provide portable and ready-to-use biosensors which can be useable by anyone anywhere around the world without requiring formal training. According to the World Health Organization (WHO), 76,000 people each year die in India alone because of pesticide poisoning. Long term exposure to organophosphate pesticides is known to have adverse effects on neurological function and can lead to Alzheimer's Disease, Attention Deficit Hyperactivity Disorder (ADHD), and reduced Intelligence Quotient (IQ). The likelihood of long term exposure to pesticides is heightened in developing countries, so a reliable and inexpensive pesticide sensor is a much-needed device in the developing world. To address this need, this project reports on the development of a fully-automated bioactive paper-based sensor for the detection of organophosphate pesticides. In the proposed biosensor, two innovations were implemented to achieve a full-automated format for the pesticide sensor: (I) First is a PUMP ON A PAPER (Jahanshahi-Anbuhi et al., LOC, 2012) that increases the flow rate of fluids within paper-based microfluidic analytical devices and sequentially brings two separate liquid streams to the enzyme test zone on the paper sensor, and (II) the second innovation is a PIPETTE ON A PAPER (Jahanshahi-Anbuhi et al., LOC, 2014) that involved the creation of a pullulan (a natural non-ionic polysaccharide) temporary bridge-system to transfer a known amount of solution to the sensing zone that, gives the enzyme zone a chance to dry and accept the substrate solution from the slow channel after a fixed period of time. This proposed format results in a simplified assay that detects the presence of pesticides automatically without any further manipulation from the user. However, the shelf life of this assay kit is challenging due to instability of both enzyme (AChE) and substrate (IDA) at room temperature. AChE loses its enzymatic activity when stored at room temperature and IDA becomes oxidized quickly. This problem is not unique to these two bio reagents, however; almost all bioassays which use bio-reagents (such as enzymes and small-molecular substrates) are unstable to varying degrees and require special shipping and storage. The instability of these molecules can arise from either thermal denaturation or chemical modification, such as oxidation or hydrolysis. Because of these issues, they often have to be shipped on dry ice with special packaging, which is costly. The cost… Advisors/Committee Members: Pelton, Robert, Filipe, Carlos D.M., Chemical Engineering.

Subjects/Keywords: Biosensor; Microfluidic; Bio-active Paper; Paper Based Microfluidic Devices; Pullulan; Pill Based Assay; Reagent Release; Enzyme Stabilization; Organophosphate Pesticide; E. coli; Luciferase; Luminescent; Lab on Pills; Pullulan Tablet; Pullulan Capsule

11 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jahanshahi-Anbuhi, S. (2014). Development of Ready-to-Use Biosensors for Diagnostics and Biosensing. (Doctoral Dissertation). McMaster University. Retrieved from http://hdl.handle.net/11375/16501

Chicago Manual of Style (16^th Edition):

Jahanshahi-Anbuhi, Sana. “Development of Ready-to-Use Biosensors for Diagnostics and Biosensing.” 2014. Doctoral Dissertation, McMaster University. Accessed December 13, 2019. http://hdl.handle.net/11375/16501.

MLA Handbook (7^th Edition):

Jahanshahi-Anbuhi, Sana. “Development of Ready-to-Use Biosensors for Diagnostics and Biosensing.” 2014. Web. 13 Dec 2019.

Vancouver:

Jahanshahi-Anbuhi S. Development of Ready-to-Use Biosensors for Diagnostics and Biosensing. [Internet] [Doctoral dissertation]. McMaster University; 2014. [cited 2019 Dec 13]. Available from: http://hdl.handle.net/11375/16501.

Council of Science Editors:

Jahanshahi-Anbuhi S. Development of Ready-to-Use Biosensors for Diagnostics and Biosensing. [Doctoral Dissertation]. McMaster University; 2014. Available from: http://hdl.handle.net/11375/16501

University of Helsinki

19. Jokinen, Nora. Assay optimization and screening for hepatitis C virus replication inhibitors from marine-derived substances.

Degree: Farmaceutiska fakulteten, 2013, University of Helsinki

URL: http://hdl.handle.net/10138/40773

► Maailmassa on arviolta 180 miljoonaa hepatiitti C -viruksen kantajaa. C-hepatiitti aiheuttaa usein oireettomia maksasairauksia. Krooniset infektiot voivat aiheuttaa maksakirroosia, johtaa elinsiirtoon tai maksasyöpään. Lääkkeiden kehitys… (more)

▼ Maailmassa on arviolta 180 miljoonaa hepatiitti C -viruksen kantajaa. C-hepatiitti aiheuttaa usein oireettomia maksasairauksia. Krooniset infektiot voivat aiheuttaa maksakirroosia, johtaa elinsiirtoon tai maksasyöpään. Lääkkeiden kehitys oli hidasta kunnes vuonna 1999 kehitettiin ensimmäinen soluissa itsenäisesti kopioituva virusmalli, joka ilmensi vain osaa koko viruksen genomista. Nykyinen lääkekehitystutkimus kohdistuu viruksen kopioitumisen kannalta tärkeisiin proteiineihin, kuten NS3/4A proteaasiin, NS3 helikaasiin, NS5A proteiiniin sekä NS5B RNA polymeraasiin. Hepatiitti C -virus kuuluu Hepasivirusten sukuun. Geneettisen vaihtelun takia tähän mennessä on tunnistettu ainakin seitsemän eri genotyyppiä ja useita alatyyppejä. Genotyyppi 1 on yleisin maailmassa, ja samalla kaikista vaikein hoitaa. Nykyinen hoito perustuu pääasiassa 24-48 viikkoa kestävään hoitoon pegyloidulla interferoni alfalla sekä nukleosidirakenteisella ribaviriinillä, joista kumpikaan ei ole erityisesti C-hepatiitin hoitoon suunniteltu lääke. Lisäksi tällä hoidolla on paljon vakavia haittavaikutuksia. Vuonna 2011 markkinoille tuli kaksi uutta suoraan C-hepatiittia vastaan kehitettyä lääkettä, proteaasin estäjät telapreviiri ja bosepreviiri. C-hepatiittia vastaan ei ole onnistuttu kehittämään vielä rokotetta. Tämän tutkimuksen tavoitteena oli optimoida ja validoida toistettava solupohjainen seulontamenetelmä antiviraalisen aktiivisuuden määritystä varten. Geneettisesti muunnellut Huh-7 solut ilmentävät vain osaa hepatiitti C -viruksen genomista, ja ne tuottavat vain kopioitumiseen tarvittavia virusproteiineja. Lisäksi genomiin oli lisätty tulikärpäsestä peräisin oleva lusiferaasigeeni, jonka tuottamaa luminesenssia mittaamalla arvioitiin virusproteiinien tuottoa. Solujen tuottaman lusiferaasin määrä on suoraan verrannollinen hepatiitti C virusproteiinien kopioitumiseen. Optimoitua ja validoitua menetelmää käytettiin hepatiitti C -viruksen kopioitumista estävien aineiden seulontaan yhdistekirjastosta, joka sisälsi merieliöperäisiä näytteitä yhteensä 113. Meren mikro- ja makro-organismit ovat viime vuosina olleet kiinnostava lähde uusien lääkkeiden etsinnässä. Tämä tutkimus oli osa kansainvälistä MAREX-hanketta, joka tähtää uusien aktiivisten molekyylien löytämiseen. Yhteensä 37 yhdistettä (32,7 %) osoittivat yli 50 % antiviraalista tehoa. Näiden yhdisteiden sytotoksisuus tutkittiin ATP:tä mittaavalla kokeella. 10 yhdistettä (27,0 %) osoittivat alle 20 % sytotoksisuutta. Näistä kuusi oli synteettisiä yhdisteitä ja neljä uutteita. Yhdisteistä, jotka osoittivat hyvää antiviraalista tehoa sekä alhaista sytotoksisuutta, tulee tutkia jatkossa niiden annos-vastesuhde. Nämä yhdisteet tulisi myös tutkia solulinjalla, joka ilmentää koko hepatiitti C -viruksen genomia. Vaikka yhdiste on ollut aktiivinen soluissa, jotka ilmentävät vain osaa hepatiitti C -viruksen genomista, viruksen rakenneproteiinit voivat kuitenkin vaikuttaa yhdisteen tehoon ja inaktivoida sen.

Subjects/Keywords: Hepatitis C virus; replication inhibitor; antiviral screening; cell-based assay; luciferase reporter; Farmakognosi; Pharmacognosy; Farmakognosia

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jokinen, N. (2013). Assay optimization and screening for hepatitis C virus replication inhibitors from marine-derived substances. (Masters Thesis). University of Helsinki. Retrieved from http://hdl.handle.net/10138/40773

Chicago Manual of Style (16^th Edition):

Jokinen, Nora. “Assay optimization and screening for hepatitis C virus replication inhibitors from marine-derived substances.” 2013. Masters Thesis, University of Helsinki. Accessed December 13, 2019. http://hdl.handle.net/10138/40773.

MLA Handbook (7^th Edition):

Jokinen, Nora. “Assay optimization and screening for hepatitis C virus replication inhibitors from marine-derived substances.” 2013. Web. 13 Dec 2019.

Vancouver:

Jokinen N. Assay optimization and screening for hepatitis C virus replication inhibitors from marine-derived substances. [Internet] [Masters thesis]. University of Helsinki; 2013. [cited 2019 Dec 13]. Available from: http://hdl.handle.net/10138/40773.

Council of Science Editors:

Jokinen N. Assay optimization and screening for hepatitis C virus replication inhibitors from marine-derived substances. [Masters Thesis]. University of Helsinki; 2013. Available from: http://hdl.handle.net/10138/40773

University of Lund

20. Kotarsky, Knut. Development of sensitive cellular assay systems and their application to the identification of "orphan" seven-transmembrane receptors.

Degree: 2003, University of Lund

URL: http://lup.lub.lu.se/record/465640 ; http://portal.research.lu.se/ws/files/5657663/1692971.pdf

► Seven-transmembrane, G-protein coupled receptors play a central role in physiology since they facilitate cell communication in multicellular organisms by recognition of a broad range of… (more)

▼ Seven-transmembrane, G-protein coupled receptors play a central role in physiology since they facilitate cell communication in multicellular organisms by recognition of a broad range of ligands. They also represent important drug targets. Unfortunately, for many of these receptors the endogenous ligands, and, hence, their physiological functions, remain to be identified. These receptors are usually referred to as “orphan” receptors. A pre-requisite for the identification of ligands activating “orphan” receptors is powerful assay systems displaying a high assay quality as specified by a high Z-value. Until now, reporter gene assays have not been in common use in this process. Therefore, we aimed to develop improved reporter gene assays. This aim was accomplished by optimizing the promoter region of the construct, the reporter enzyme, and the assay procedure. Furthermore, a fluorescence-based clone selection step was introduced, that allowed the selection of the most sensitive reporter cell clones. The established test cell lines responded sensitively upon stimulation of various cell surface receptors as demonstrated by several receptors. In the first approach, transcription of the reporter gene was under control of a synthetic promoter consisting of 9 TPA responsive elements. In a further improved construct, the promoter was extended with six NF-kB and six STAT motifs. The used reporter gene was designed as a fusion gene coding for green fluorescent protein and Photinus luciferase. The amplification of reporter enzyme activity was substantially larger than in any other described system, and the high assay quality made it suitable as a primary screening tool. The development of efficient assays allowed the screening for hitherto unknown ligands to orphan seven-transmembrane receptors. Natural ligands for two recently unknown receptors were identified. Thus, we identified the second leukotriene B4 receptor, BLT2, and the first cell surface, free fatty acid receptor, FFA1. The BLT2 receptor was first identified in silico, cloned, and subsequently functionally expressed in HeLa cells. The identification of FFA1 was accomplished using a reverse pharmacology approach. The FFA1 receptor (previously known as GPR40) responded to medium to long chain free fatty acids, including compounds like ±9-hydroxy-octadecadienoic acid (9-HODE) and a conjugated linoleic acid (10,12-CLA). Receptor expression was detected in heart, skeletal muscle, liver and in pancreatic b-cells. Most importantly, the identification of anti-diabetic drugs (thiazolidinediones and MEDICA16) as agents acting on this receptor implies an important connection between FFA1R and type II diabetes.

Subjects/Keywords: Neurovetenskaper; Biokemi; Metabolism; Biochemistry; metabolism; leukotrieneB4; thiazolidinedione; 9-HODE; fatty acid; luciferase; GFP; reporter gene; preclinical assay; receptor; GPCR

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kotarsky, K. (2003). Development of sensitive cellular assay systems and their application to the identification of "orphan" seven-transmembrane receptors. (Doctoral Dissertation). University of Lund. Retrieved from http://lup.lub.lu.se/record/465640 ; http://portal.research.lu.se/ws/files/5657663/1692971.pdf

Chicago Manual of Style (16^th Edition):

Kotarsky, Knut. “Development of sensitive cellular assay systems and their application to the identification of "orphan" seven-transmembrane receptors.” 2003. Doctoral Dissertation, University of Lund. Accessed December 13, 2019. http://lup.lub.lu.se/record/465640 ; http://portal.research.lu.se/ws/files/5657663/1692971.pdf.

MLA Handbook (7^th Edition):

Kotarsky, Knut. “Development of sensitive cellular assay systems and their application to the identification of "orphan" seven-transmembrane receptors.” 2003. Web. 13 Dec 2019.

Vancouver:

Kotarsky K. Development of sensitive cellular assay systems and their application to the identification of "orphan" seven-transmembrane receptors. [Internet] [Doctoral dissertation]. University of Lund; 2003. [cited 2019 Dec 13]. Available from: http://lup.lub.lu.se/record/465640 ; http://portal.research.lu.se/ws/files/5657663/1692971.pdf.

Council of Science Editors:

Kotarsky K. Development of sensitive cellular assay systems and their application to the identification of "orphan" seven-transmembrane receptors. [Doctoral Dissertation]. University of Lund; 2003. Available from: http://lup.lub.lu.se/record/465640 ; http://portal.research.lu.se/ws/files/5657663/1692971.pdf

Tampere University

21. Lahtinen, Suvi. Optimizing a method to reliably detect the furin activity at cellular level and identify the regulators of its activity .

Degree: 2015, Tampere University

URL: https://trepo.tuni.fi/handle/10024/97773

Subjects/Keywords: furin; S2 cells; direct cell assay; RNA-interference; genome-wide screening; luciferase reporter gene

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lahtinen, S. (2015). Optimizing a method to reliably detect the furin activity at cellular level and identify the regulators of its activity . (Masters Thesis). Tampere University. Retrieved from https://trepo.tuni.fi/handle/10024/97773

Chicago Manual of Style (16^th Edition):

Lahtinen, Suvi. “Optimizing a method to reliably detect the furin activity at cellular level and identify the regulators of its activity .” 2015. Masters Thesis, Tampere University. Accessed December 13, 2019. https://trepo.tuni.fi/handle/10024/97773.

MLA Handbook (7^th Edition):

Lahtinen, Suvi. “Optimizing a method to reliably detect the furin activity at cellular level and identify the regulators of its activity .” 2015. Web. 13 Dec 2019.

Vancouver:

Lahtinen S. Optimizing a method to reliably detect the furin activity at cellular level and identify the regulators of its activity . [Internet] [Masters thesis]. Tampere University; 2015. [cited 2019 Dec 13]. Available from: https://trepo.tuni.fi/handle/10024/97773.

Council of Science Editors:

Lahtinen S. Optimizing a method to reliably detect the furin activity at cellular level and identify the regulators of its activity . [Masters Thesis]. Tampere University; 2015. Available from: https://trepo.tuni.fi/handle/10024/97773

Queensland University of Technology

22. Lai, John. Functional analyses of polymorphisms in the promoters of the KLK3 and KLK4 genes in prostate cancer.

Degree: 2006, Queensland University of Technology

URL: https://eprints.qut.edu.au/16454/

► This PhD aimed to elucidate the mechanisms by which polymorphisms may alter androgen-induced transactivation of androgen receptor (AR) target genes which may be important in… (more)

▼ This PhD aimed to elucidate the mechanisms by which polymorphisms may alter androgen-induced transactivation of androgen receptor (AR) target genes which may be important in prostate cancer aetiology. The second aspect of this PhD focused on identifying and characterising functional polymorphisms that may have utility as predictive risk indicators for prostate cancer and which may aid in earlier therapeutic intervention and better disease management. Analyses were carried out on the kallikrein-related peptidase 3 (KLK3), also known as the prostate specific antigen (PSA), gene and the kallikrein-related peptidase 4 (KLK4) gene. The PSA and KLK4 genes are part of the serine protease family that have trypsin or chymotrypsin like activity and are thought to play a role in the development of hormone-dependent cancers in tissues such as those in the prostate, breast, endometrium and ovaries. In the prostate, PSA is regulated by androgens and three androgen response elements (AREs) have been described in the promoter and upstream enhancer region. The PSA ARE I harbours a polymorphism at -158 bp from the transcription initiation site (TIS) that results in a G to A transition (G-158A). This PhD investigated the functional significance of the PSA G-158A polymorphism which has been reported to be associated with prostate cancer risk. Electromobility shift assays (EMSAs) investigating the interaction of ARE I variants with the AR DNA binding domain (AR-DBD) demonstrated that the A allele had a two-fold increased binding affinity for the AR-DBD when compared with the G allele. This was confirmed with endogenous AR in limited proteolysis-EMSA experiments. The limited proteolysis-EMSA experiments also demonstrated differential sensitivities of PSA ARE I alleles to trypsin digestion, which suggests that the G-158A polymorphism has an allosteric effect on the AR that alters AR/ARE I complex stability. Furthermore, Chromatin Immunoprecipitation (ChIP) assays suggest that the A allele more readily recruited the AR in vivo when compared with the G allele and is consistent with the in vitro binding data. Luciferase reporter assays carried out in both LNCaP and 22Rv1 prostate cancer cells, and using the natural (dihydrotestosterone; DHT) ligand demonstrated that the A allele was more responsive to androgens in LNCaP cells. Hence, this study has elucidated the potential mechanisms by which the G-158A polymorphism may differentially regulate PSA expression (of which up-regulation of PSA is thought to be important in prostate cancer development and progression). KLK4 has similar tissue-restricted expression as PSA and is up-regulated by steroid hormones in many endocrine cells including those in the prostate. A putative ARE (KLK4-pARE) located at -1,005 to -1019 relative to the more predominantly used transcription initiation site, TIS3, was initially found in supershift assays using AR antibodies to interact with endogenous AR. However, subsequent EMSA analysis using purified AR-DBD suggest that KLK4-pARE may be interacting with the…

Subjects/Keywords: prostate cancer; single nucleotide polymorphism (SNP); kallikreins (KLKs); kallikrein 4 (KLK4); prostate specific antigen (PSA); androgens; hormones; androgen receptor (AR); androgen receptor DNA binding domain (AR-DBD); androgen response element (ARE); electromobility shift assay (EMSA); luciferase reporter assay

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lai, J. (2006). Functional analyses of polymorphisms in the promoters of the KLK3 and KLK4 genes in prostate cancer. (Thesis). Queensland University of Technology. Retrieved from https://eprints.qut.edu.au/16454/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lai, John. “Functional analyses of polymorphisms in the promoters of the KLK3 and KLK4 genes in prostate cancer.” 2006. Thesis, Queensland University of Technology. Accessed December 13, 2019. https://eprints.qut.edu.au/16454/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lai, John. “Functional analyses of polymorphisms in the promoters of the KLK3 and KLK4 genes in prostate cancer.” 2006. Web. 13 Dec 2019.

Vancouver:

Lai J. Functional analyses of polymorphisms in the promoters of the KLK3 and KLK4 genes in prostate cancer. [Internet] [Thesis]. Queensland University of Technology; 2006. [cited 2019 Dec 13]. Available from: https://eprints.qut.edu.au/16454/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lai J. Functional analyses of polymorphisms in the promoters of the KLK3 and KLK4 genes in prostate cancer. [Thesis]. Queensland University of Technology; 2006. Available from: https://eprints.qut.edu.au/16454/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Northeastern University

23. Lau, Joyce Yuk-Ting. Evolution and function of the globin gene complexes of Antarctic Dragonfishes (Notothenioidei: Bathydraconidae).

Degree: MS, Department of Biology, 2009, Northeastern University

URL: http://hdl.handle.net/2047/d20000336

► As the Southern Ocean cooled to -1.8°C over the past 40 million years, the teleostean clade Notothenioidei became less reliant on hemoglobin and red blood… (more)

▼ As the Southern Ocean cooled to -1.8°C over the past 40 million years, the teleostean clade Notothenioidei became less reliant on hemoglobin and red blood cells, a trend which culminates in the white-blooded, erythrocyte-null Antarctic icefishes. The adult alpha and beta globin genes of red-blooded notothenioids are linked in 5'-to-5' orientation such that the intergenic sequences direct divergent transcription of the loci. To gain further insight into their evolution and function, we have compared the intergenic regions of the adult alpha/beta globin gene complexes from three species of Antarctic rockcods (Nototheniidae), a basal notothenioid group, and from eight species of Antarctic dragonfishes (Bathydraconidae), the red-blooded notothenioids most closely related to the icefishes, as well as the alpha and beta globin of 13 species and 10 genera of dragonfish. The ancestral nototheniid intergene appears to be ~3 kb in length, although one species contains a duplication of ~1 kb. The bathydraconid intergenes resolve into three distinct subclasses [long (3.8 kb), intermediate (3.0 kb), and short (1.5-2.3 kb)] that correspond to the subfamilies proposed for the taxon: Gymonodraconinae, Bathydraconinae, and Cygnodraconinae. Using luciferase reporter technology, we assessed the promoter/enhancer activities of the intergenes from the rockcod Notothenia coriiceps and the dragonfishes Akarotaxis nudiceps (Bathydraconinae) and Gerlachea australis (Cygnodraconinae) in the erythropoietic microenvironment of differentiated MEL cells. We found that the N. coriiceps intergene directs high-level transcription in both orientations, the A. nudiceps intergene is active only in alpha orientation, and the short intergene of G. australis supports only weak transcription. Phylogenetic analysis indicated that dragonfish are paraphyletic. Our alpha/beta globin, and intergenic data agree with the subfamilies proposed by mitochondrial data but Cygnodraconinae and Gymnodraconinae are more closely related and Bathydraconinae should be split into two subclades. The icefish representative N. ionah was found to have diverged from the dragonfish, after the initial split of the dragonfish family such that N. ionah is more closely related to Cygnodraconinae and Gymnodraconinae. Studies of the beta globin gene resulted in the discovery of pseudogenes in a number of dragonfish. This raises the question as of multiplicity of beta globin in dragonfish as well as other notothenioids. Our results show that dragonfish can be resolved into four groups, with icefish more related to a subset of the dragonfish family. Functional and phylogenetic results are consistent with the hypothesis that the notothenioid globin loci are in evolutionary flux, probably due to relaxation of selection pressure for hemoglobin expression.

Subjects/Keywords: alpha/beta globin intergenic regulatory region; Bathydraconidae; luciferase reporter assay; murine erythroleukemia cell (MEL); Nototheniidae; notothenioid fish suborder; Pterois volitans – Evolution; Biology

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APA (6^th Edition):

Lau, J. Y. (2009). Evolution and function of the globin gene complexes of Antarctic Dragonfishes (Notothenioidei: Bathydraconidae). (Masters Thesis). Northeastern University. Retrieved from http://hdl.handle.net/2047/d20000336

Chicago Manual of Style (16^th Edition):

Lau, Joyce Yuk-Ting. “Evolution and function of the globin gene complexes of Antarctic Dragonfishes (Notothenioidei: Bathydraconidae).” 2009. Masters Thesis, Northeastern University. Accessed December 13, 2019. http://hdl.handle.net/2047/d20000336.

MLA Handbook (7^th Edition):

Lau, Joyce Yuk-Ting. “Evolution and function of the globin gene complexes of Antarctic Dragonfishes (Notothenioidei: Bathydraconidae).” 2009. Web. 13 Dec 2019.

Vancouver:

Lau JY. Evolution and function of the globin gene complexes of Antarctic Dragonfishes (Notothenioidei: Bathydraconidae). [Internet] [Masters thesis]. Northeastern University; 2009. [cited 2019 Dec 13]. Available from: http://hdl.handle.net/2047/d20000336.

Council of Science Editors:

Lau JY. Evolution and function of the globin gene complexes of Antarctic Dragonfishes (Notothenioidei: Bathydraconidae). [Masters Thesis]. Northeastern University; 2009. Available from: http://hdl.handle.net/2047/d20000336

24. Lind, Liza. Non-coding RNA in T cell activation and function.

Degree: Faculty of Health Sciences, 2013, Linköping UniversityLinköping University

URL: http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-96966

► For a long time research has focused on the protein-coding mRNA, but there is a complex world of non-coding RNAs regulating the human body… (more)

▼ For a long time research has focused on the protein-coding mRNA, but there is a complex world of non-coding RNAs regulating the human body that we yet know very little about. Non-coding RNAs (ncRNAs) are involved in modulation of different cell processes including proliferation, differentiation and apoptosis. In the current study the role of ncRNAs in T cell activation and function was investigated. T cells are important mediators of immune responses, for example upon viral infections. The T helper cells (TH or CD4+ cells) are involved in orchestrating immune processes like aiding the activation of macrophages and enhancement of B cell function. The TH1 cell subtype is generally pro-inflammatory and IFNγ-secreting. There are regulatory T (Treg) cells that are involved in downregulation of TH1 cells, to decrease or terminate the immune response. It has been shown that upon repeated stimulation TH1 cells can switch into a Treg-like IL10-secreting anti-inflammatory phenotype. In the IL10-secreting Treg-like cells the microRNA 150 (miR-150) was found upregulated compared to IFNγ-secreting TH1 cells. Thus, miR-150 was believed to be a candidate in key regulation of the switch between the two phenotypes. Predicted target genes of miR-150 were identified using mRNA arrays investigating down-regulated genes in the IL10-secreting Treg-like subpopulation. In this thesis predicted targets of miR-150 were investigated using luciferase assays. Unfortunately no targets were identified. Upon isolation of IFNγ-secreting TH1 cells and Treg-like IL10-secreting cells, it was found that the ncRNA 886 (nc886) was upregulated in these activated cells, compared to resting TH cells. This indicates that nc886 has an important role in T cell activation. Nc886 has been shown to inhibit PKR activation in other cell types. The effect of nc886 on protein kinase R (PKR) was therefore investigated. PKR shuts down translation upon activation in response to viral double-stranded RNA or cellular stress. We showed that in an activated T cell phenotype nc886 is affecting PKR upon activation by dsRNA from HIV or synthetic origin. The PKR activation pattern is reversed in a resting T cell phenotype.

Subjects/Keywords: ncRNA 886; non-coding RNA 886; microRNA 150; miRNA 150; PKR; T cell activation; luciferase assay

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APA (6^th Edition):

Lind, L. (2013). Non-coding RNA in T cell activation and function. (Thesis). Linköping UniversityLinköping University. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-96966

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lind, Liza. “Non-coding RNA in T cell activation and function.” 2013. Thesis, Linköping UniversityLinköping University. Accessed December 13, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-96966.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lind, Liza. “Non-coding RNA in T cell activation and function.” 2013. Web. 13 Dec 2019.

Vancouver:

Lind L. Non-coding RNA in T cell activation and function. [Internet] [Thesis]. Linköping UniversityLinköping University; 2013. [cited 2019 Dec 13]. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-96966.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lind L. Non-coding RNA in T cell activation and function. [Thesis]. Linköping UniversityLinköping University; 2013. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-96966

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universitat Pompeu Fabra

25. Muiños Gimeno, Margarita. Analysis of genetic variation in microrna-mediated regulation and the susceptibility to anxiety disorders.

Degree: Departament de Ciències Experimentals i de la Salut, 2009, Universitat Pompeu Fabra

URL: http://hdl.handle.net/10803/7192

► Hem investigat la variació genètica a la regulació mediada per microRNAs com a factors de susceptibilitat pels trastorns d'ansietat seguint dues aproximacions diferents. Primer vam… (more)

Subjects/Keywords: isoforma; anàlisi de transcriptoma; PCR; assaig de luciferasa; variant comuna; variant rara; reseqüenciar; estudis d'associació; poly-miRTS; trastorn obsessiu-compulsiu; trastorn de pànic; trastorn d'ansietat; variació genètica intraespecífica; SNP; NTRK3; gen candidat; element regulador; microRNA; variació genòmica; desordres poligènics; trets complexes; isoform; transcriptome analysis; PCR; luciferase assay; common variant; rare variant; re-sequencing; association studies; poly-miRTS; obsessive-compulsive disorder; panic disorder; anxiety disorder; genetic variation within species; SNP; NTRK3; candidate gene; regulatory element; microRNA; genome variation; polygenic disorders; complex traits; 57

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Muiños Gimeno, M. (2009). Analysis of genetic variation in microrna-mediated regulation and the susceptibility to anxiety disorders. (Thesis). Universitat Pompeu Fabra. Retrieved from http://hdl.handle.net/10803/7192

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Muiños Gimeno, Margarita. “Analysis of genetic variation in microrna-mediated regulation and the susceptibility to anxiety disorders.” 2009. Thesis, Universitat Pompeu Fabra. Accessed December 13, 2019. http://hdl.handle.net/10803/7192.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Muiños Gimeno, Margarita. “Analysis of genetic variation in microrna-mediated regulation and the susceptibility to anxiety disorders.” 2009. Web. 13 Dec 2019.

Vancouver:

Muiños Gimeno M. Analysis of genetic variation in microrna-mediated regulation and the susceptibility to anxiety disorders. [Internet] [Thesis]. Universitat Pompeu Fabra; 2009. [cited 2019 Dec 13]. Available from: http://hdl.handle.net/10803/7192.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Muiños Gimeno M. Analysis of genetic variation in microrna-mediated regulation and the susceptibility to anxiety disorders. [Thesis]. Universitat Pompeu Fabra; 2009. Available from: http://hdl.handle.net/10803/7192

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

26. Muthusamy, Sasikumar. Evaluation of interaction and combined toxicity of polyaromatic hydrocarbons (PAHs) and metals using human cell-based bioassays.

Degree: School of Medicine, 2016, University of Queensland

URL: http://espace.library.uq.edu.au/view/UQ:409659

Subjects/Keywords: Mixture toxicity; In vitro assays; Cytotoxicity; Oxidative stress; Genotoxicity; Flow cytometry; Luciferase assay; Reporter gene system; Regulatory toxicology; Health risk assessment; 050204 Environmental Impact Assessment; 0601 Biochemistry and Cell Biology; 111506 Toxicology (incl.Clinical Toxicology)

…genotoxicity, flow cytometry, luciferase assay, reporter gene system, regulatory toxicology, health… …luciferase assay CAFLUX- chemically activated fluorescent assay CAS- chemical abstract registry Cd… …23 2.8.1. Luciferase Assays… …31 3.4. Cytotoxicity assay… …37 4.2.3. Cytotoxicity assay…

11 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Muthusamy, S. (2016). Evaluation of interaction and combined toxicity of polyaromatic hydrocarbons (PAHs) and metals using human cell-based bioassays. (Thesis). University of Queensland. Retrieved from http://espace.library.uq.edu.au/view/UQ:409659

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Muthusamy, Sasikumar. “Evaluation of interaction and combined toxicity of polyaromatic hydrocarbons (PAHs) and metals using human cell-based bioassays.” 2016. Thesis, University of Queensland. Accessed December 13, 2019. http://espace.library.uq.edu.au/view/UQ:409659.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Muthusamy, Sasikumar. “Evaluation of interaction and combined toxicity of polyaromatic hydrocarbons (PAHs) and metals using human cell-based bioassays.” 2016. Web. 13 Dec 2019.

Vancouver:

Muthusamy S. Evaluation of interaction and combined toxicity of polyaromatic hydrocarbons (PAHs) and metals using human cell-based bioassays. [Internet] [Thesis]. University of Queensland; 2016. [cited 2019 Dec 13]. Available from: http://espace.library.uq.edu.au/view/UQ:409659.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Muthusamy S. Evaluation of interaction and combined toxicity of polyaromatic hydrocarbons (PAHs) and metals using human cell-based bioassays. [Thesis]. University of Queensland; 2016. Available from: http://espace.library.uq.edu.au/view/UQ:409659

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Texas Medical Branch – Galveston

27. [No author]. Targeting protein: protein interaction sites for drug development against voltage-gated sodium channels .

Degree: University of Texas Medical Branch – Galveston

URL: http://hdl.handle.net/2152.3/11197

► Voltage-gated sodium (Nav) channels are responsible for initiation and propagation of action potentials, which contribute to control of neuronal excitability. Malfunction of specific Nav channel… (more)

▼ Voltage-gated sodium (Nav) channels are responsible for initiation and propagation of action potentials, which contribute to control of neuronal excitability. Malfunction of specific Nav channel isoforms is associated with a wide range of brain disorders including psychological, neurological and developmental disorders. Unfortunately, currently available drugs targeting Nav channels are directed against highly conserved domains of the α-subunit of all Nav channels, and as such they have severe side effects, including cardiac malfunction. Fortunately, the macromolecular complex of Nav channels is a source of less conserved protein-protein interaction (PPI) interfaces that represent a novel opportunity for designing isoform-specific chemical leads targeting Nav channels. The macromolecular complex of Nav channels is regulated by a number of accessory proteins. Very few proteins regulate the functional properties of Nav channels as potently as the intracellular fibroblast growth factor 14 (FGF14). FGF14 is a biologically relevant accessory protein of the neuronal Nav channel complex controlling gating, stability, and trafficking of native Nav channels. Through a monomeric interaction with the intracellular C-terminal tail of Nav channel α subunits, FGF14 binds and modulates the activity of Nav channels in an isoform-specific manner. By applying luciferase-based assays, patch clamp electrophysiology, and intrinsic fluorescence studies, we have identified the β9 loop at the interface of FGF14 as critical for binding to Nav1.6 channels. Based on this information, we have identified a short sequence on FGF14 and designed a peptidomimetic (ZL181) fragment as an effective probe for modulating Nav1.6 channels as measured by luciferase-based assay. This peptidomimetic was further evaluated with purified proteins, in silico docking, and whole-cell patch clamp electrophysiology in both in vitro and ex vivo systems. Overall, our data demonstrated that a novel peptidomimetic (ZL181) can modulate the functional properties of Nav1.6 channels and can suppress neuronal excitability in nucleus accumbens medium spiny neurons. The new knowledge gained from this study might be useful for the treatment of Nav1.6 channel-related brain disorders such as epilepsy, schizophrenia and cognitive disorders. Advisors/Committee Members: Laezza, Fernanda (advisor), Pettitt, B. Montgomery (committeeMember), Zhou, Jia (committeeMember), Tempia, Filippo (committeeMember), Stoilova-McPhie, Svetla (committeeMember).

Subjects/Keywords: FGF14; Fibroblast growth factor 14 Nav channel; Voltage-gated sodium channel; PPI; Protein: protein interactions; LCA; Luciferase complementation assay; Small Peptide

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

author], [. (n.d.). Targeting protein: protein interaction sites for drug development against voltage-gated sodium channels . (Thesis). University of Texas Medical Branch – Galveston. Retrieved from http://hdl.handle.net/2152.3/11197

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

author], [No. “Targeting protein: protein interaction sites for drug development against voltage-gated sodium channels .” Thesis, University of Texas Medical Branch – Galveston. Accessed December 13, 2019. http://hdl.handle.net/2152.3/11197.

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

author], [No. “Targeting protein: protein interaction sites for drug development against voltage-gated sodium channels .” Web. 13 Dec 2019.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

author] [. Targeting protein: protein interaction sites for drug development against voltage-gated sodium channels . [Internet] [Thesis]. University of Texas Medical Branch – Galveston; [cited 2019 Dec 13]. Available from: http://hdl.handle.net/2152.3/11197.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

Council of Science Editors:

author] [. Targeting protein: protein interaction sites for drug development against voltage-gated sodium channels . [Thesis]. University of Texas Medical Branch – Galveston; Available from: http://hdl.handle.net/2152.3/11197

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

University of Texas Medical Branch – Galveston

28. [No author]. Degradational and Transcriptional Investigations of the RAF Kinase Inhibitory Protein .

Degree: University of Texas Medical Branch – Galveston

URL: http://hdl.handle.net/2152.3/801

► RKIP (Raf-1 kinase inhibitory protein) is a novel potent metastasis suppressor by its function of inhibiting ERK pathway, NF-κB pathway, and GRK-2. RKIP expression level… (more)

Subjects/Keywords: RKIP; degradation; transcription; phosphorylation; ubiquitination; luciferase assay

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

author], [. (n.d.). Degradational and Transcriptional Investigations of the RAF Kinase Inhibitory Protein . (Thesis). University of Texas Medical Branch – Galveston. Retrieved from http://hdl.handle.net/2152.3/801

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

author], [No. “Degradational and Transcriptional Investigations of the RAF Kinase Inhibitory Protein .” Thesis, University of Texas Medical Branch – Galveston. Accessed December 13, 2019. http://hdl.handle.net/2152.3/801.

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

author], [No. “Degradational and Transcriptional Investigations of the RAF Kinase Inhibitory Protein .” Web. 13 Dec 2019.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

author] [. Degradational and Transcriptional Investigations of the RAF Kinase Inhibitory Protein . [Internet] [Thesis]. University of Texas Medical Branch – Galveston; [cited 2019 Dec 13]. Available from: http://hdl.handle.net/2152.3/801.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

Council of Science Editors:

author] [. Degradational and Transcriptional Investigations of the RAF Kinase Inhibitory Protein . [Thesis]. University of Texas Medical Branch – Galveston; Available from: http://hdl.handle.net/2152.3/801

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

University of Oxford

29. Prapansilp, Panote. Molecular pathological investigation of the pathophysiology of fatal malaria.

Degree: PhD, 2012, University of Oxford

URL: http://ora.ox.ac.uk/objects/uuid:e966a2f2-a37d-4586-b09e-2bb616e5dce2 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.658384

► Malaria remains one of the world's major health problems, especially in developing countries. A better understanding of the pathology and pathophysiology of severe malaria is… (more)

▼ Malaria remains one of the world's major health problems, especially in developing countries. A better understanding of the pathology and pathophysiology of severe malaria is key to develop new treatments. Different approaches have been used in malaria research including the in vitro co-culture models with endothelial cells and both murine and simian animal models. However these are open to controversy due to disagreement on their representativeness of human disease. Using human post-mortem tissue in malaria research is another important approach but is practically challenging, limiting the availability of post mortem samples from malaria patients. The work in this thesis had two main themes. First I examined the role of the endothelial signalling Angiopoetin-Tie-2 receptor pathway in malaria. Ang-2 has been shown to be a significant biomarker of severe and fatal malaria. I examined the tissue specific expression of proteins from this pathway in post-mortem brain tissues from fatal malaria cases, but found no difference between cerebral malaria and non-cerebral malaria cases. Ang-2 correlated with the severity of malaria in these patients. An attempt to examine the interaction of hypoxia and the Ang-Tie-2 pathway in vitro using a co-culture model of human brain endothelial cells was unsuccessful due to contamination of the cell line. The second part of the thesis aimed to utilise molecular pathology techniques including miRNA and whole-genome microarrays. I have shown for the first time that these can be successfully applied to human post-mortem tissue in malaria. First I used archival tissues to examine the microRNA signature in the kidney of patients with malaria associated renal failure. Second I optimised a protocol to preserve post mortem tissue for molecular pathology, from an autopsy study in Mozambique. Using the subsequent total mRNA transcriptomic data and bioinformatics analysis this work has expanded our knowledge of differential gene expression and the families of genes which are dysregulated in the brain in response to malaria infection.

Subjects/Keywords: 616.9; Malaria; Pathology; Tropical medicine; Cerebral malaria; Infectious diseases; molecular pathology; autopsy; tissue quality; african; asian; angiopoietin; tie-2; hypoxia; cell culture; acute kidney injury; malaria associated acute renal failure; microRNA; microarray; cloning; luciferase assay

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Prapansilp, P. (2012). Molecular pathological investigation of the pathophysiology of fatal malaria. (Doctoral Dissertation). University of Oxford. Retrieved from http://ora.ox.ac.uk/objects/uuid:e966a2f2-a37d-4586-b09e-2bb616e5dce2 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.658384

Chicago Manual of Style (16^th Edition):

Prapansilp, Panote. “Molecular pathological investigation of the pathophysiology of fatal malaria.” 2012. Doctoral Dissertation, University of Oxford. Accessed December 13, 2019. http://ora.ox.ac.uk/objects/uuid:e966a2f2-a37d-4586-b09e-2bb616e5dce2 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.658384.

MLA Handbook (7^th Edition):

Prapansilp, Panote. “Molecular pathological investigation of the pathophysiology of fatal malaria.” 2012. Web. 13 Dec 2019.

Vancouver:

Prapansilp P. Molecular pathological investigation of the pathophysiology of fatal malaria. [Internet] [Doctoral dissertation]. University of Oxford; 2012. [cited 2019 Dec 13]. Available from: http://ora.ox.ac.uk/objects/uuid:e966a2f2-a37d-4586-b09e-2bb616e5dce2 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.658384.

Council of Science Editors:

Prapansilp P. Molecular pathological investigation of the pathophysiology of fatal malaria. [Doctoral Dissertation]. University of Oxford; 2012. Available from: http://ora.ox.ac.uk/objects/uuid:e966a2f2-a37d-4586-b09e-2bb616e5dce2 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.658384

University of Queensland

30. Pretorius, Lara-Simone. Investigation of virus-induced defence modulations during plant-virus interactions.

Degree: School of Agriculture & Food Sciences, 2018, University of Queensland

URL: http://espace.library.uq.edu.au/view/UQ:8

Subjects/Keywords: Biogenesis pathway; Defence pathways; Dual-luciferase assay; Gene expression; Jasmonic acid; med18; Small RNAs; Turnip mosaic virus; Viral evolution; 0604 Genetics; 0605 Microbiology; 0607 Plant Biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pretorius, L. (2018). Investigation of virus-induced defence modulations during plant-virus interactions. (Thesis). University of Queensland. Retrieved from http://espace.library.uq.edu.au/view/UQ:8

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pretorius, Lara-Simone. “Investigation of virus-induced defence modulations during plant-virus interactions.” 2018. Thesis, University of Queensland. Accessed December 13, 2019. http://espace.library.uq.edu.au/view/UQ:8.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pretorius, Lara-Simone. “Investigation of virus-induced defence modulations during plant-virus interactions.” 2018. Web. 13 Dec 2019.

Vancouver:

Pretorius L. Investigation of virus-induced defence modulations during plant-virus interactions. [Internet] [Thesis]. University of Queensland; 2018. [cited 2019 Dec 13]. Available from: http://espace.library.uq.edu.au/view/UQ:8.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pretorius L. Investigation of virus-induced defence modulations during plant-virus interactions. [Thesis]. University of Queensland; 2018. Available from: http://espace.library.uq.edu.au/view/UQ:8

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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Open Access Theses and Dissertations