subject:( Germplasm)

Louisiana State University

1. Cuevas Uribe, Rafael. A general approach for vitrification of fish sperm.

Degree: PhD, Environmental Sciences, 2011, Louisiana State University

URL: etd-08252011-163658 ; https://digitalcommons.lsu.edu/gradschool_dissertations/3597

► The goal of this project was to develop streamlined protocols that could be integrated into a standardized approach for vitrification of germplasm for all aquatic… (more)

▼ The goal of this project was to develop streamlined protocols that could be integrated into a standardized approach for vitrification of germplasm for all aquatic species. Vitrification (freezing by formation of “glass” rather than crystalline ice) is simple, fast, inexpensive, can be potentially used to preserve samples in the field, and offers new options for germplasm management especially appropriate for small fishes. Sperm were studied from freshwater fish (channel catfish Ictalurus punctatus), viviparous freshwater fish (green swordtail Xiphophorus hellerii), and marine fishes (spotted seatrout Cynoscion nebulosus, red snapper Lutjanus campechanus, red drum Sciaenops ocellatus, and southern flounder Paralichthys lethostigma). To reduce toxicity, combinations of cryoprotectants at reduced concentrations with incorporation of trehalose and polymers were used to enhance glass formation. For freezing, samples were suspended on 10-µL polystyrene loops and plunged into liquid nitrogen. Thawing was done at 24ºC using Hanks’ balanced salt solution at 300 mOsmol/kg for freshwater species, and seawater at 1,020 mOsmol/kg for marine species. Quality after vitrification was evaluated by sperm motility, membrane integrity and when possible fertility. Post-thaw motility of sperm in marine fishes was higher (as high as 70%) than in freshwater fishes (as high as 20%). The percentage of membrane-intact sperm for marine fishes was ~20% except for southern flounder (11%). For freshwater fishes, the percentage of membrane-intact sperm for swordtail was low (<12%) compared to channel catfish (~50%). Adaptations by marine fish to high osmotic pressures could explain the survival in the high cryoprotectant concentrations (40 – 60%) required for vitrification. This research yielded the first successful vitrification of sperm in these fishes and production of offspring from vitrified sperm in channel catfish, green swordtail, and southern flounder. Sperm vitrification offers an alternative approach to conventional cryopreservation for conservation of valuable genetic lineages, such as endangered species, model strains used in research, and improved farmed strains. Furthermore, sperm vitrification could be used to transport cryopreserved sperm from the field to the laboratory to expand genetic resources available for germplasm repositories. This technique could be utilized to reconstitute genetic lines, and as a new option for conservation biology in imperiled aquatic species.

Subjects/Keywords: germplasm; cryopreservation; conservation

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APA (6^th Edition):

Cuevas Uribe, R. (2011). A general approach for vitrification of fish sperm. (Doctoral Dissertation). Louisiana State University. Retrieved from etd-08252011-163658 ; https://digitalcommons.lsu.edu/gradschool_dissertations/3597

Chicago Manual of Style (16^th Edition):

Cuevas Uribe, Rafael. “A general approach for vitrification of fish sperm.” 2011. Doctoral Dissertation, Louisiana State University. Accessed January 19, 2021. etd-08252011-163658 ; https://digitalcommons.lsu.edu/gradschool_dissertations/3597.

MLA Handbook (7^th Edition):

Cuevas Uribe, Rafael. “A general approach for vitrification of fish sperm.” 2011. Web. 19 Jan 2021.

Vancouver:

Cuevas Uribe R. A general approach for vitrification of fish sperm. [Internet] [Doctoral dissertation]. Louisiana State University; 2011. [cited 2021 Jan 19]. Available from: etd-08252011-163658 ; https://digitalcommons.lsu.edu/gradschool_dissertations/3597.

Council of Science Editors:

Cuevas Uribe R. A general approach for vitrification of fish sperm. [Doctoral Dissertation]. Louisiana State University; 2011. Available from: etd-08252011-163658 ; https://digitalcommons.lsu.edu/gradschool_dissertations/3597

University of Zambia

2. Syamuyoba, Phillip. Characterization of Zambian Pigeonpea(Cajanus cajan (L.)Millsp)germplasm using Morphological Characters .

Degree: 2011, University of Zambia

URL: http://hdl.handle.net/123456789/254

► Pigeonpea (Cajanus cajan (L.) Millsp) is of great importance in Zambia for its diversified use, which can lead to increased farm income and subsequently food… (more)

▼ Pigeonpea (Cajanus cajan (L.) Millsp) is of great importance in Zambia for its diversified use, which can lead to increased farm income and subsequently food security at household level. Its seeds have a protein content of approximately 21% and therefore is a cheap source of protein in the human diet. The National Genebank, which has a responsibility for collecting and conserving all locally available crop germplasm, has a collection of accessions of pigeonpea that have not been characterized. Lack of characterization to obtain genetic information about these accessions has caused the germplasm not to be widely utilized by end-users such as plant breeders in the breeding programs and the magnitude of genetic variation has so far not been assessed. The objective of this study was to assess genetic variation among accessions of pigeonpea conserved at the National Genebank and to determine characters that are useful in classifying pigeonpea. Twenty-eight accessions were assessed for genetic variation based on morphological characters at Mount Makulu Central Research Station, Chilanga, Zambia during the 2000/2001 growing season. The design of the experiment was a single block and each accession was planted on a single unreplicated plot. Twenty-three characters (plant height, number of branches, leaf size, days to 50% flowering, days to 80% maturity, number of racemes, seeds per pod, 100-seed weight, growth habit, stem colour, stem thickness, leaf hairiness, leaflet shape, flower colour, second flower colour, pattern of streaks, flowering pattern, pod colour, pod hairiness, pod form, seed colour pattern, seed colour and seed shape) were measured following IBPGR/ICRISAT (1993) pigeonpea descriptor list and analysed using cluster analysis and principal component analysis. Cluster analysis grouped the accessions into four major clusters based on commonly shared characters. Three principal components were found to explain 73.9% of the total morphological variation. Days to 50% flowering, days to 80% maturity, number of branches, number of racemes, plant height, number of seeds per pod, stem colour, flower colour, pod colour, colour of the seed coat and flowering pattern were the major sources of variation among the accessions. Based on these results, it is concluded that there is genetic variation among the accessions of pigeonpea conserved at the National Genebank. It is further concluded that characters that were the major sources of variation are useful in classifying pigeonpea germplasm. These results could be useful in pigeonpea improvement programs.

Subjects/Keywords: Pigeonpea; Morphology; Germplasm Resources

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APA (6^th Edition):

Syamuyoba, P. (2011). Characterization of Zambian Pigeonpea(Cajanus cajan (L.)Millsp)germplasm using Morphological Characters . (Thesis). University of Zambia. Retrieved from http://hdl.handle.net/123456789/254

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Syamuyoba, Phillip. “Characterization of Zambian Pigeonpea(Cajanus cajan (L.)Millsp)germplasm using Morphological Characters .” 2011. Thesis, University of Zambia. Accessed January 19, 2021. http://hdl.handle.net/123456789/254.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Syamuyoba, Phillip. “Characterization of Zambian Pigeonpea(Cajanus cajan (L.)Millsp)germplasm using Morphological Characters .” 2011. Web. 19 Jan 2021.

Vancouver:

Syamuyoba P. Characterization of Zambian Pigeonpea(Cajanus cajan (L.)Millsp)germplasm using Morphological Characters . [Internet] [Thesis]. University of Zambia; 2011. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/123456789/254.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Syamuyoba P. Characterization of Zambian Pigeonpea(Cajanus cajan (L.)Millsp)germplasm using Morphological Characters . [Thesis]. University of Zambia; 2011. Available from: http://hdl.handle.net/123456789/254

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas A&M University

3. Gerrish, Brandon James. Screening Texas A&M Germplasm and Environments for Hybrid Wheat Potential.

Degree: MS, Plant Breeding, 2015, Texas A&M University

URL: http://hdl.handle.net/1969.1/156289

► Higher wheat prices, performance potential of hybrids, and the availability of new next generation sequencing has sparked a renewed interest in the development of hybrid… (more)

▼ Higher wheat prices, performance potential of hybrids, and the availability of new next generation sequencing has sparked a renewed interest in the development of hybrid wheat. The main advantages of hybrids are higher biomass and yield, longer grain fill periods, enhanced yield stability, vigorous root systems, and increased resistance to biotic and abiotic stresses. The purpose of this research was to investigate key components that are necessary for a hybrid wheat program. The objectives of this study were to 1) gain a better understanding of the environments and germplasm by utilizing yield data from 2008-2012 advanced variety trials and biplot analysis, 2) determine the contribution of parents in early observation nursery and advanced yield trials by utilizing the existing data from 2009-2012 and 2011-2014 data, respectively, and 3) estimate heterosis and combining ability among a selected set of TAM lines based on phenotypic traits. The third objective was achieved by evaluating the F1 generation from a half-diallel cross for yield and its components in a growth chamber. The biplot analysis of yield data from 2008-2012 advanced variety trials showed high significant differences amongst environments, varieties, and variety-by-environment interaction. Three mega-environments within Texas were identified and several environments were found to be potentially suitable for hybrid wheat production as they produced high yields each year. ‘Duster’ was found to be the highest yielding and most stable cultivar across environments while ‘Fannin’ was the lowest yielding and unstable. ‘TAM 112’ and ‘TAM 111’ were among the top parental contributors in developing new lines, while ‘Pastor’ had the best mean yield performance among cultivars. The F1 generation from the diallel cross showed significant differences (P<0.05) in all factors of yield and its components. Three of the eight parents (TAM 113, TAM 305, TAM 401) were found to have highly significant (P<0.005) positive general combining ability (GCA) effects for grain yield while three others (TAM 111, TX10D2230, Sturdy 2K) were found to have highly negative GCA effects. In the F2 generation, significant differences (P<0.05) were found for grain yield. None of the parents were found to be significant for GCA. Advisors/Committee Members: Ibrahim, Amir M.H. (advisor), Rudd, Jackie C. (committee member), Liu, Shuyu (committee member).

Subjects/Keywords: Screening; Germplasm; Environments; Hybrid; Wheat

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APA (6^th Edition):

Gerrish, B. J. (2015). Screening Texas A&M Germplasm and Environments for Hybrid Wheat Potential. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/156289

Chicago Manual of Style (16^th Edition):

Gerrish, Brandon James. “Screening Texas A&M Germplasm and Environments for Hybrid Wheat Potential.” 2015. Masters Thesis, Texas A&M University. Accessed January 19, 2021. http://hdl.handle.net/1969.1/156289.

MLA Handbook (7^th Edition):

Gerrish, Brandon James. “Screening Texas A&M Germplasm and Environments for Hybrid Wheat Potential.” 2015. Web. 19 Jan 2021.

Vancouver:

Gerrish BJ. Screening Texas A&M Germplasm and Environments for Hybrid Wheat Potential. [Internet] [Masters thesis]. Texas A&M University; 2015. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1969.1/156289.

Council of Science Editors:

Gerrish BJ. Screening Texas A&M Germplasm and Environments for Hybrid Wheat Potential. [Masters Thesis]. Texas A&M University; 2015. Available from: http://hdl.handle.net/1969.1/156289

University of Saskatchewan

4. Dhanda, Rohit Kumar. Fatty acid composition in diverse oat germplasm.

Degree: 2011, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-03202011-224522

► Oat is an important crop for livestock feed and human food. Increased interest in the health promoting properties of oat has led to a need… (more)

▼ Oat is an important crop for livestock feed and human food. Increased interest in the health promoting properties of oat has led to a need to explore diverse oat germplasm for improved nutritional quality. One target for improved nutritional quality could be an altered fatty acid composition. A study was conducted to explore the fatty acid profile of diverse accessions from the world oat collection preserved in the Canadian national seed genebank, Plant Gene Resources of Canada (PGRC), at the Agriculture and Agri-Food Canada (AAFC) Research Centre, Saskatoon, Saskatchewan, Canada and genotypes from the Crop Development Centre (CDC), University of Saskatchewan, Saskatoon and the Eastern Cereal and Oilseed Research Centre (ECORC), AAFC, Ottawa, Canada. Accessions included a wide range of Avena sativa L. and other selected species from the genus Avena (A. byzantina C. Koch, A. sterilis L., A. fatua L., A. sativa subsp. nudisativa (Husn.) Rod. et. Sold. and A. strigosa Schreb.). The fatty acid profiles of 917 oat accessions from these taxa were analyzed using gas chromatography, revealing significant variability for the three major fatty acids in oat oil. Oleic and linoleic acid demonstrated the greatest variation. A few A. sativa accessions had higher oleic and lower palmitic acid levels compared to the general average. Some hexaploid wild oat accessions (A. sterilis) showed relatively high oleic and below average levels of palmitic and linoleic acid compared to A. sativa. A. strigosa accessions had consistently higher levels of oleic acid than other Avena species. Based on initial results, 52 selected A. sativa accessions were grown in 2009 in replicated field trials and re-evaluated to gain insight into the influence of the growing environment on fatty acid composition. Fatty acid composition was affected by genotype, whereas location significantly affected palmitic and oleic acid content. Correlations were determined among the contents of the six fatty acids, oil content and protein content. Oleic acid content was positively correlated with oil content, which may be particularly important to plant breeders for nutritional quality improvement of future oat cultivars. The understanding gained from this research suggests the possibility of improving the fatty acid profile of future oat cultivars for food and feed. Advisors/Committee Members: Diederichsen,Axel, Rossnagel, Brian, Tyler, Bob, Bett, Kirstin.

Subjects/Keywords: Fatty acid composition; Germplasm; Oat

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APA (6^th Edition):

Dhanda, R. K. (2011). Fatty acid composition in diverse oat germplasm. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-03202011-224522

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dhanda, Rohit Kumar. “Fatty acid composition in diverse oat germplasm.” 2011. Thesis, University of Saskatchewan. Accessed January 19, 2021. http://hdl.handle.net/10388/etd-03202011-224522.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dhanda, Rohit Kumar. “Fatty acid composition in diverse oat germplasm.” 2011. Web. 19 Jan 2021.

Vancouver:

Dhanda RK. Fatty acid composition in diverse oat germplasm. [Internet] [Thesis]. University of Saskatchewan; 2011. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10388/etd-03202011-224522.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dhanda RK. Fatty acid composition in diverse oat germplasm. [Thesis]. University of Saskatchewan; 2011. Available from: http://hdl.handle.net/10388/etd-03202011-224522

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

5. Dezern, Stanley W. Phenotypic and Biochemical Characterization of the United States Department of Agriculture Core Peanut (Arachis Hypogaea L.) Germplasm Collection.

Degree: MS, Agronomy, 2018, University of Florida

URL: https://ufdc.ufl.edu/UFE0052244

► Peanut (Arachis hypogaea L.) is an economically important leguminous oilseed crop. Low levels of genetic diversity have been observed in the peanut genome due to… (more)

▼ Peanut (Arachis hypogaea L.) is an economically important leguminous oilseed crop. Low levels of genetic diversity have been observed in the peanut genome due to the genetic similarity of the two diploid progenitors, the relatively recent evolution of A. hypogaea (2.3-2.9 million years ago), the highly self-pollinating nature of peanut reproduction, and the impact of intense selection by humans using peanut as a crop for thousands of years. To maintain the limited genetic variability availability in cultivated peanut, the USDA peanut germplasm collection was created, maintaining around 10,000 unique accessions. To allow breeders more access to the large collection, a core collection was developed in 1993 containing 831 unique accessions chosen by clustering and randomly sampling the accessions in the full collection. A mini-core collection was later developed using similar methods, but resulting in 112 of the core accessions. The core and mini-core collections have been shown effective in breeding, but the accessions within have not been fully characterized across phenotypic and biochemical traits in a single experiment. This study aimed to collect and analyze phenotypic and biochemical data from the core and mini-core collections in a single augmented design study. Phenotypic descriptors were collected representing agronomic performance, standard industry descriptors, plant architecture, leaf structure, pod and seed traits, and flowering pattern. Additionally, biochemical data was collected for protein content, oil content, and oil fatty acid composition. High levels of variation were found across most traits in the core collection, with generally less diversity observed in the mini-core collection. A not unsubstantial number of accessions in the core and mini-core collection did not display the main stem flowering pattern based on their subspecies designation. A series of canonical discriminate analysis of phenotypic characteristics indicated that these accessions are not grouped correctly by market type, differing significantly across canonical variables compared to the correctly flowering accessions of the market type. The core collection showed a large amount of variation across biochemical traits, notably in oleic acid and linoleic acid content, as well as in minor fatty acids. Biochemical data further supported the revision of market type designation. These findings can help to identify inconsistencies in the core and mini-core collections and provide robust data for QTL analysis. ( en ) Advisors/Committee Members: MACDONALD,GREGORY E (committee chair), MULVANEY,MICHAEL J (committee member), HOLBROOK,CARL C,JR (committee member), BARKLEY,NOELLE (committee member).

Subjects/Keywords: core – germplasm – peanut – phenotype

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APA (6^th Edition):

Dezern, S. W. (2018). Phenotypic and Biochemical Characterization of the United States Department of Agriculture Core Peanut (Arachis Hypogaea L.) Germplasm Collection. (Masters Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/UFE0052244

Chicago Manual of Style (16^th Edition):

Dezern, Stanley W. “Phenotypic and Biochemical Characterization of the United States Department of Agriculture Core Peanut (Arachis Hypogaea L.) Germplasm Collection.” 2018. Masters Thesis, University of Florida. Accessed January 19, 2021. https://ufdc.ufl.edu/UFE0052244.

MLA Handbook (7^th Edition):

Dezern, Stanley W. “Phenotypic and Biochemical Characterization of the United States Department of Agriculture Core Peanut (Arachis Hypogaea L.) Germplasm Collection.” 2018. Web. 19 Jan 2021.

Vancouver:

Dezern SW. Phenotypic and Biochemical Characterization of the United States Department of Agriculture Core Peanut (Arachis Hypogaea L.) Germplasm Collection. [Internet] [Masters thesis]. University of Florida; 2018. [cited 2021 Jan 19]. Available from: https://ufdc.ufl.edu/UFE0052244.

Council of Science Editors:

Dezern SW. Phenotypic and Biochemical Characterization of the United States Department of Agriculture Core Peanut (Arachis Hypogaea L.) Germplasm Collection. [Masters Thesis]. University of Florida; 2018. Available from: https://ufdc.ufl.edu/UFE0052244

University of Minnesota

6. Altendorf, Kayla. Characterization of Field Pennycress (Thlaspi arvense L.) Germplasm for Use as a Cover Crop and Biofuel Feedstock.

Degree: MS, Applied Plant Sciences, 2017, University of Minnesota

URL: http://hdl.handle.net/11299/194663

► Field pennycress (Thlaspi arvense L.) is a winter annual oilseed crop currently under investigation for use as a feedstock for domestic biofuel production. As an… (more)

Subjects/Keywords: Characterization; Germplasm; Pennycress; Thlaspi arvense

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APA (6^th Edition):

Altendorf, K. (2017). Characterization of Field Pennycress (Thlaspi arvense L.) Germplasm for Use as a Cover Crop and Biofuel Feedstock. (Masters Thesis). University of Minnesota. Retrieved from http://hdl.handle.net/11299/194663

Chicago Manual of Style (16^th Edition):

Altendorf, Kayla. “Characterization of Field Pennycress (Thlaspi arvense L.) Germplasm for Use as a Cover Crop and Biofuel Feedstock.” 2017. Masters Thesis, University of Minnesota. Accessed January 19, 2021. http://hdl.handle.net/11299/194663.

MLA Handbook (7^th Edition):

Altendorf, Kayla. “Characterization of Field Pennycress (Thlaspi arvense L.) Germplasm for Use as a Cover Crop and Biofuel Feedstock.” 2017. Web. 19 Jan 2021.

Vancouver:

Altendorf K. Characterization of Field Pennycress (Thlaspi arvense L.) Germplasm for Use as a Cover Crop and Biofuel Feedstock. [Internet] [Masters thesis]. University of Minnesota; 2017. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/11299/194663.

Council of Science Editors:

Altendorf K. Characterization of Field Pennycress (Thlaspi arvense L.) Germplasm for Use as a Cover Crop and Biofuel Feedstock. [Masters Thesis]. University of Minnesota; 2017. Available from: http://hdl.handle.net/11299/194663

Louisiana State University

7. Childress, William Martin. On-site Sperm Cryopreservation for Aquatic Repository Development and User Group Linkage.

Degree: MS, Environmental Sciences, 2017, Louisiana State University

URL: etd-05232017-085339 ; https://digitalcommons.lsu.edu/gradschool_theses/4577

► Although on-site cryopreservation of aquatic species sperm in nitrogen-vapor shipping dewars has been used for more than 30 yr, many limitations remain. Due to the… (more)

▼ Although on-site cryopreservation of aquatic species sperm in nitrogen-vapor shipping dewars has been used for more than 30 yr, many limitations remain. Due to the size of shipping dewars, most studies are small scale and can only produce tens of samples. The freezing temperatures that can be achieved are also affected by the size of the dewar and the number of samples being frozen due to the heat load. In addition, often due to timing or remote location these samples cannot be shipped, and if they are shipped, quality can be lost and the samples must be discarded. The goal of this thesis was to create a mobile aquatic cryopreservation laboratory that could operate at research and commercial-scale capacities and, could function under multiple scenarios in different on-site environments with a high level of quality control. There were three operational designs identified for the mobile laboratory: 1) self-contained work inside the unit using generator power; 2) work inside the unit using external facilities power; 3) using the equipment inside a host facility. Computer-aided design software was used to model a 3.8 m long trailer and various internal components to evaluate different configurations. From the final design, the layout inside the mobile laboratory was constructed and initially tested at the LSUAC Aquatic Germplasm and Genetic Resource Center. This demonstrated that the portable generator provided sufficient power for simultaneous use of all equipment. The capitol and variable costs were documented of construction and integration of the mobile laboratory into an existing cryopreservation facility at different levels of automation. This led to blueprints and layout information that can provide guidance for others to build their own units. Finally, the mobile laboratory was deployed into the field to cryopreserve sperm from Blue Catfish, Xiphophorus spp., and Red Snapper. This resulted in processing of 684 males and 6,838 French straw being produced. This work provides a general approach for high-throughput on-site cryopreservation of aquatic species for repository development and user group linkage. Germplasm repositories would allow the reconstitution of species or populations, and linking users could help remove standardization and quality control bottlenecks.

Subjects/Keywords: Cryopreservation; mobile; Aquatic; Germplasm

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APA (6^th Edition):

Childress, W. M. (2017). On-site Sperm Cryopreservation for Aquatic Repository Development and User Group Linkage. (Masters Thesis). Louisiana State University. Retrieved from etd-05232017-085339 ; https://digitalcommons.lsu.edu/gradschool_theses/4577

Chicago Manual of Style (16^th Edition):

Childress, William Martin. “On-site Sperm Cryopreservation for Aquatic Repository Development and User Group Linkage.” 2017. Masters Thesis, Louisiana State University. Accessed January 19, 2021. etd-05232017-085339 ; https://digitalcommons.lsu.edu/gradschool_theses/4577.

MLA Handbook (7^th Edition):

Childress, William Martin. “On-site Sperm Cryopreservation for Aquatic Repository Development and User Group Linkage.” 2017. Web. 19 Jan 2021.

Vancouver:

Childress WM. On-site Sperm Cryopreservation for Aquatic Repository Development and User Group Linkage. [Internet] [Masters thesis]. Louisiana State University; 2017. [cited 2021 Jan 19]. Available from: etd-05232017-085339 ; https://digitalcommons.lsu.edu/gradschool_theses/4577.

Council of Science Editors:

Childress WM. On-site Sperm Cryopreservation for Aquatic Repository Development and User Group Linkage. [Masters Thesis]. Louisiana State University; 2017. Available from: etd-05232017-085339 ; https://digitalcommons.lsu.edu/gradschool_theses/4577

Rutgers University

8. Chen, Hui, 1989-. Traffic tolerance of fine fescues: techniques for screening germplasm.

Degree: PhD, Plant Biology, 2020, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/63083/

► The term fine fescue refers to several Festuca spp. that have a very fine leaf texture compared to most other turfgrass species. These species are… (more)

▼ The term fine fescue refers to several Festuca spp. that have a very fine leaf texture compared to most other turfgrass species. These species are adapted to low-input management systems and have been used in mixtures with other cool-season grasses. However, fine fescues are not utilized to the same extent as other species partially due to their poor traffic tolerance and recuperative ability. Improvement in traffic tolerance of fine fescues would enable use of these grasses beyond turf systems that experience little to no traffic. The purpose of this dissertation was to develop and evaluate germplasm screening techniques that improve selecting efficiency for traffic tolerant fine fescues. The specific objectives of this research were: i) to evaluate the effect of traffic form (abrasive wear vs. cleated traffic) and season (spring vs. summer vs. autumn) on the assessment of fine fescue traffic tolerance (Chapters 1 and 2); ii) to evaluate the effect of nitrogen fertilization and harvest time on cell wall composition of fine fescues (Chapters 3 and 4); iii) to investigate the correlation between cell wall composition and wear tolerance of fine fescues; and iv) to develop near-infrared reflectance spectroscopy (NIRS) models to determine the cell wall composition of fine fescues. For the first objective, the ability of fine fescue turf to maintain a dense cover depended on the specific traffic form and varied based on the season during which wear stress occurred. Abrasive wear, applied with Rutgers Wear Simulator, caused more thinning of the turf canopy than cleated traffic applied with the Cady Traffic Simulator. Thus, abrasive wear resulted in a greater separation among fine fescues based on the fullness of turf cover (FTC) and will likely improve selection efficiency compared to cleated traffic. The FTC response of fine fescues were more sensitive to traffic stress during summer because of the high disease pressure and heat stress. Screening for fine fescues for improved traffic tolerance during spring would probably be less biased and more effective at identifying tolerance to traffic within fine fescues because other abiotic or biotic stresses would be avoided or minimized. Abrasive wear was also effective in identifying cultivars that are susceptible to leaf bruising. Leaf bruising was more severe during summer and autumn due to the heat stress. However, leaf bruising response of fine fescue cultivars varied with the season; sheep fescue and strong creeping red fescue were more susceptible to leaf bruising during summer while Chewings fescue and slender creeping red fescue were more bruised during autumn. Thus, evaluation of this characteristic would need to be conducted in both the summer and autumn. For the second objective, N fertilization was expected to alter cell wall composition as it promotes new leafy growth, which would be expected to have lower cell wall content. However, the effect of N fertilization on cell wall composition was not observed. Differences among three fine fescue species were… Advisors/Committee Members: Murphy, James A. (chair), Helsel, Zane R. (internal member), Bonos, Stacy A. (internal member), Ward, Daniel L. (internal member), Westendorf, Michael L. (outside member), School of Graduate Studies.

Subjects/Keywords: Fine fescue; Fescue – Germplasm resources

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APA (6^th Edition):

Chen, Hui, 1. (2020). Traffic tolerance of fine fescues: techniques for screening germplasm. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/63083/

Chicago Manual of Style (16^th Edition):

Chen, Hui, 1989-. “Traffic tolerance of fine fescues: techniques for screening germplasm.” 2020. Doctoral Dissertation, Rutgers University. Accessed January 19, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/63083/.

MLA Handbook (7^th Edition):

Chen, Hui, 1989-. “Traffic tolerance of fine fescues: techniques for screening germplasm.” 2020. Web. 19 Jan 2021.

Vancouver:

Chen, Hui 1. Traffic tolerance of fine fescues: techniques for screening germplasm. [Internet] [Doctoral dissertation]. Rutgers University; 2020. [cited 2021 Jan 19]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/63083/.

Council of Science Editors:

Chen, Hui 1. Traffic tolerance of fine fescues: techniques for screening germplasm. [Doctoral Dissertation]. Rutgers University; 2020. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/63083/

University of Zambia

9. Nkhoma, Nelia. Stability of yield and Antioxidant content of selected advanced Cowpea(Vigna unguiculata [L]Walp.) mutation derived lines .

Degree: 2014, University of Zambia

URL: http://hdl.handle.net/123456789/3413

► Zambia has a number of cowpea germplasm which are high yielding and contain antioxidants which are useful for preventing a lot of health problems i.e.… (more)

▼ Zambia has a number of cowpea germplasm which are high yielding and contain antioxidants which are useful for preventing a lot of health problems i.e. Heart attack, Hypertension, Obesity and Cancer. Unfortunately the production suitability of these germplasm in the different areas of the country is not known. Based on this, a study was conducted to evaluate the stability of cowpea yield and antioxidants (total phenolic content and condensed tannin content) in the three agro ecological zones as well as to determine the relationship between the antioxidants and the seed coat colour of the cowpeas. Multilocation field trials involving ten cowpea genotypes were conducted at three different Agro-ecological Regions. A randomized complete block design was employed with 3 replications. Cowpea grain yield and antioxidant contents of the seed were determined and a stability bases analysis tool, Additive Main effect and Multiplicative Model (AMMI) was employed for data analysis. Assessment of genotype x environment (GxE) interaction on cowpea grain yield stability indicated that GxE was not present for yield indicating that genotypes did not respond differently to varying environmental conditions. However, some genotypes had higher yields than others indicating genotype identification to specific environments. Genotypes MS1/8/1/4 and LT11/3/3/12 were adapted to high potential yielding environment and were unstable while BB4/2/4/1 and LT11/5/2/2 were adapted to low yielding environment and were stable environments. Assessment for antioxidants showed that GxE was significant (p<0.01) and higher yielding genotypes had low antioxidant contents compared to low yielding genotypes. Genotype LT PRT had higher antioxidant concentration (3.47mg/100mgCE) and stable (IPCA2 0.022) while MS PRT had lower concentrations (0.17mg/100mgCE) and unstable (IPCA1 0.630). The genotypes which had higher antioxidant concentrations had darker seed coat colour (yellowish brown and purplish brown) compared to the ones which had low antioxidant concentration (white). This study identified stable genotypes in both yield and antioxidants. However, further studies for assessing yield stability are necessary and could be achieved by including more seasons and sites to get a better understanding of GxE and yield stability of cowpea in Zambia.

Subjects/Keywords: Cowpea-Germplasm Resources; Plant Breeding; Cowpea-Genetics

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APA (6^th Edition):

Nkhoma, N. (2014). Stability of yield and Antioxidant content of selected advanced Cowpea(Vigna unguiculata [L]Walp.) mutation derived lines . (Thesis). University of Zambia. Retrieved from http://hdl.handle.net/123456789/3413

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Nkhoma, Nelia. “Stability of yield and Antioxidant content of selected advanced Cowpea(Vigna unguiculata [L]Walp.) mutation derived lines .” 2014. Thesis, University of Zambia. Accessed January 19, 2021. http://hdl.handle.net/123456789/3413.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Nkhoma, Nelia. “Stability of yield and Antioxidant content of selected advanced Cowpea(Vigna unguiculata [L]Walp.) mutation derived lines .” 2014. Web. 19 Jan 2021.

Vancouver:

Nkhoma N. Stability of yield and Antioxidant content of selected advanced Cowpea(Vigna unguiculata [L]Walp.) mutation derived lines . [Internet] [Thesis]. University of Zambia; 2014. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/123456789/3413.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nkhoma N. Stability of yield and Antioxidant content of selected advanced Cowpea(Vigna unguiculata [L]Walp.) mutation derived lines . [Thesis]. University of Zambia; 2014. Available from: http://hdl.handle.net/123456789/3413

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University

10. Uchendu, Esther Eyiuche. Cryopreservation of Shoot Tips: Antioxidant Investigations with Rubus and Protocols for Mentha and Vaccinium.

Degree: PhD, Horticulture, 2009, Oregon State University

URL: http://hdl.handle.net/1957/12731

► Oxidative stress that occurs during cryopreservation may be reduced by the addition of antioxidants. Vitamin E (Vit E), Vitamin C (Vit C), glutathione (GSH), lipoic… (more)

▼ Oxidative stress that occurs during cryopreservation may be reduced by the addition of antioxidants. Vitamin E (Vit E), Vitamin C (Vit C), glutathione (GSH), lipoic acid (LA), glycine betaine (GB) and polyvinylpyrrolidone (PVP) were applied at four steps of the PVS2 vitrification protocol (pretreatment, loading, rinsing, and regrowth). Shoot tips of in-vitro grown blackberry cultivars "Chehalem‟ and "Hull Thornless‟ were cryopreserved. Malondialdehyde (MDA), a lipid peroxidation product was higher in shoot tips at each step compared to controls. Shoot tips treated with Vit E had low MDA similar to the controls. GB, GSH, Vit E and Vit C significantly increased regrowth at all steps. Vit C added to regrowth medium with double Murashige and Skoog iron concentration decreased recovery; however, in iron-free medium Vit C improved regrowth. LA was not very effective in regrowth medium. PVP was not effective at any step. Regrowth was highest (up to 92%) with Vit C. This is the first report of vitamins C, and E, GB, and LA use in protecting cultures from oxidative damage during cryopreservation. Mentha species [M. canadensis L., M. australis R.Br., Mentha x piperita citrata (Ehrh.) Briq. and M. cunninghamii Benth,] were cryopreserved using controlled cooling (CC), encapsulation dehydration (ED), and PVS2 vitrification (VIT). Recovery of Mentha x piperita citrata and M. australis showed significant differences among protocols with CC > VIT > ED. M. canadensis and M. cunninghamii recovery with CC and ED was significantly better than VIT. All species responded to CC (93%) better than ED (71%) or VIT (73%). Cranberry (Vaccinium macrocarpon Aiton) cultivars "Wilcox‟ and "Franklin‟ and blueberry (Vaccinium corymbosum L.) "Berkeley‟, "O'Neal‟ and "Brigitta‟ were tested for desiccation tolerance and recovery after cryopreservation. Blueberry cultivars were desiccation tolerant after 7 h of drying under laminar air flow while cranberry cultivars were mostly dead by 3 h. Cryopreserved blueberry cultivars had 83% to 92% regrowth with ED. The results varied from 33% to 87% for VIT and from 50% to 67% for CC. Cranberry cultivars had poor regrowth with these protocols but improved by 20-30% with Vit C added at any of the four PVS2 steps. Advisors/Committee Members: Reed, Barbara M. (advisor), Chen, Tony H.H. (committee member).

Subjects/Keywords: Vitrification; Mints (Plants) – Germplasm resources – Cryopreservation

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APA (6^th Edition):

Uchendu, E. E. (2009). Cryopreservation of Shoot Tips: Antioxidant Investigations with Rubus and Protocols for Mentha and Vaccinium. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/12731

Chicago Manual of Style (16^th Edition):

Uchendu, Esther Eyiuche. “Cryopreservation of Shoot Tips: Antioxidant Investigations with Rubus and Protocols for Mentha and Vaccinium.” 2009. Doctoral Dissertation, Oregon State University. Accessed January 19, 2021. http://hdl.handle.net/1957/12731.

MLA Handbook (7^th Edition):

Uchendu, Esther Eyiuche. “Cryopreservation of Shoot Tips: Antioxidant Investigations with Rubus and Protocols for Mentha and Vaccinium.” 2009. Web. 19 Jan 2021.

Vancouver:

Uchendu EE. Cryopreservation of Shoot Tips: Antioxidant Investigations with Rubus and Protocols for Mentha and Vaccinium. [Internet] [Doctoral dissertation]. Oregon State University; 2009. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1957/12731.

Council of Science Editors:

Uchendu EE. Cryopreservation of Shoot Tips: Antioxidant Investigations with Rubus and Protocols for Mentha and Vaccinium. [Doctoral Dissertation]. Oregon State University; 2009. Available from: http://hdl.handle.net/1957/12731

Cornell University

11. Gutierrez, Benjamin Leo. DIHYDROCHALCONES IN MALUS MILL. GERMPLASM AND HYBRID POPULATIONS.

Degree: PhD, Plant Breeding, 2017, Cornell University

URL: http://hdl.handle.net/1813/59019

► Dihydrochalcones are abundant in Malus Mill. species, including the cultivated apple (M. ×domestica Borkh.). Phloridzin, the primary dihydrochalcone in Malus species, has beneficial nutritional qualities,… (more)

▼ Dihydrochalcones are abundant in Malus Mill. species, including the cultivated apple (M. ×domestica Borkh.). Phloridzin, the primary dihydrochalcone in Malus species, has beneficial nutritional qualities, including antioxidant, anti-cancer, and anti-diabetic properties. As such, phloridzin could be a target for improvement of nutritional quality in new apple cultivars. In addition to phloridzin, a few rare Malus species produce trilobatin or sieboldin in place of phloridzin and hybridization can lead to combinations of phloridzin, trilobatin, or sieboldin in interspecific apple progenies. Trilobatin and sieboldin also have unique chemical properties that make them desirable targets for apple breeding, including high antioxidant activity, anti-inflammatory, anti-diabetic properties, and a high sweetness intensity. We studied the variation of phloridzin, sieboldin, and trilobatin content in leaves of 377 accessions from the USDA National Plant Germplasm System (NPGS) Malus collection in Geneva, NY over three seasons and identified valuable genetic resources for breeding and researching dihydrochalcones. From these resources, five apple hybrid populations were developed to determine the genetic basis of dihydrochalcone variation. Phloridzin, sieboldin, and trilobatin appear to follow segregation patterns for three independent genes and significant trait-marker associations were identified using genetic data from genotyping-by-sequencing. Dihydrochalcones are at much lower quantities in mature apple fruit compared with vegetative tissues. Within the fruit, phloridzin is more concentrated in the peel than in the flesh. We observed higher phloridzin content in the peel of russet apples compared to the peel of non-russet apples. Russet is a disorder affecting the development of the fruit cuticle, causing a smooth waxy surface to be replaced by a rough, russet colored layer. We compared leaf, and fruit peel and flesh samples from 108 accessions in the USDA-NPGS Malus collection and identified a strong correlation between percent russeting and phloridzin content the fruit peel, especially in sport families with variation for russeting. Though russeting can severely impact commercial value of apple cultivars, russeted fruit have a unique nutritional profile compared to non-russet cultivars. Advisors/Committee Members: Brown, Susan K. (chair), Cheng, Lailiang (committee member), Gore, Michael Allen (committee member), Zhong, Gan-Yuan (committee member).

Subjects/Keywords: Genetics; Apple; Dihydrochalcones; GBS; Germplasm; Malus; Phloridzin

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gutierrez, B. L. (2017). DIHYDROCHALCONES IN MALUS MILL. GERMPLASM AND HYBRID POPULATIONS. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/59019

Chicago Manual of Style (16^th Edition):

Gutierrez, Benjamin Leo. “DIHYDROCHALCONES IN MALUS MILL. GERMPLASM AND HYBRID POPULATIONS.” 2017. Doctoral Dissertation, Cornell University. Accessed January 19, 2021. http://hdl.handle.net/1813/59019.

MLA Handbook (7^th Edition):

Gutierrez, Benjamin Leo. “DIHYDROCHALCONES IN MALUS MILL. GERMPLASM AND HYBRID POPULATIONS.” 2017. Web. 19 Jan 2021.

Vancouver:

Gutierrez BL. DIHYDROCHALCONES IN MALUS MILL. GERMPLASM AND HYBRID POPULATIONS. [Internet] [Doctoral dissertation]. Cornell University; 2017. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1813/59019.

Council of Science Editors:

Gutierrez BL. DIHYDROCHALCONES IN MALUS MILL. GERMPLASM AND HYBRID POPULATIONS. [Doctoral Dissertation]. Cornell University; 2017. Available from: http://hdl.handle.net/1813/59019

University of Nairobi

12. Ngugi, K. Analysis of the Molecular Diversity of Kenyan Sorghum Germplasm Using Microsatellites .

Degree: 2012, University of Nairobi

URL: http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/14500

► The aim of this study was to estimate the extent of the genetic diversity present in sorghum germplasm grown in Kenya using simple sequence repeat… (more)

Subjects/Keywords: genetic diversity; germplasm; Simple Sequence Repeats; sorghum

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APA (6^th Edition):

Ngugi, K. (2012). Analysis of the Molecular Diversity of Kenyan Sorghum Germplasm Using Microsatellites . (Thesis). University of Nairobi. Retrieved from http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/14500

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ngugi, K. “Analysis of the Molecular Diversity of Kenyan Sorghum Germplasm Using Microsatellites .” 2012. Thesis, University of Nairobi. Accessed January 19, 2021. http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/14500.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ngugi, K. “Analysis of the Molecular Diversity of Kenyan Sorghum Germplasm Using Microsatellites .” 2012. Web. 19 Jan 2021.

Vancouver:

Ngugi K. Analysis of the Molecular Diversity of Kenyan Sorghum Germplasm Using Microsatellites . [Internet] [Thesis]. University of Nairobi; 2012. [cited 2021 Jan 19]. Available from: http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/14500.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ngugi K. Analysis of the Molecular Diversity of Kenyan Sorghum Germplasm Using Microsatellites . [Thesis]. University of Nairobi; 2012. Available from: http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/14500

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of KwaZulu-Natal

13. Mathew, Isack. Pre-breeding of wheat (Triticum aestivum L.) for Biomass allocation and drought tolerance.

Degree: 2018, University of KwaZulu-Natal

URL: https://researchspace.ukzn.ac.za/handle/10413/17063

► Bread wheat (Triticum aestivum L., 2n=6x=42) is the third most important cereal crop globally after maize and rice. However, its production and productivity is affected… (more)

▼ Bread wheat (Triticum aestivum L., 2n=6x=42) is the third most important cereal crop globally after maize and rice. However, its production and productivity is affected by recurrent drought and declining soil fertility. Wheat cultivars with a well-balanced biomass allocation and improved root systems have better water- and nutrient-use efficiency and, hence, increased productivity under dry-land farming systems. The overall objective of this study was to develop breeding populations of wheat with enhanced drought tolerance and biomass allocation under water-limited conditions. The specific objectives of the study were: (i) to evaluate agronomic performance and quantify biomass production and allocation between roots and shoots in selected wheat genotypes in response to different soil water levels to select promising genotypes for breeding for drought tolerance and carbon (C) sequestration, (ii) to determine variance components and heritability of biomass allocation and grain yield related traits among 99 genotypes of bread wheat and triticale (Triticosecale Wittmack) to optimize biomass partitioning for drought tolerance, (iii) to deduce the population structure and genome-wide marker-trait association of yield and biomass allocation traits in wheat to facilitate marker-assisted selection for drought tolerance and C sequestration, and (iv) to estimate the combining ability of selected wheat genotypes and their progenies for agronomic traits, biomass allocation and yield under drought-stressed and non-stressed conditions for future breeding and genetic advancement for drought tolerance and C sequestration. To achieve these objectives, different experiments were conducted. In the first study, 99 wheat genotypes and one triticale accession were evaluated under drought-stressed and non-stressed conditions in the field and greenhouse using a 10×10 alpha lattice design with two replications. Data on the following phenotypic traits were collected: days to heading (DTH), number of productive tillers per plant (NPT), plant height (PH), days to maturity (DTM), spike length (SL), thousand kernel weight (TKW), root and shoot biomass (RB and SB), root to shoot ratio (RS) and grain yield (GY). There was significant (p<0.05) genotypic variation for grain yield and biomass production. The highest grain yield of 247.3 g m-2 was recorded in the genotype LM52 and the least was in genotype Sossognon with 30 g m-2. Shoot biomass ranged from 830g m-2 (genotype Arenza) to 437 g m-2 (LM57), whilst root biomass ranged between 140 g m-2 for LM15 and 603 g m-2 for triticale. Triticale also recorded the highest RS of 1.2, while the least was 0.30 for LM18. Water stress reduced total biomass production by 35% and RS by 14%. Genotypic variation existed for all measured traits useful for improving drought tolerance, while the calculated RS values can improve accuracy in estimating C sequestration potential of wheat. The following genotypes: BW140, BW141, BW152, BW162, LM26, LM47, LM48, LM71, LM70 and LM75 were selected for further development… Advisors/Committee Members: Shimelis, Hussein Ali. (advisor), Chaplot, Vincent. (advisor).

Subjects/Keywords: Germplasm.; Phenotyping.; Genotyping.; DArTseq SNP filtering.

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APA (6^th Edition):

Mathew, I. (2018). Pre-breeding of wheat (Triticum aestivum L.) for Biomass allocation and drought tolerance. (Thesis). University of KwaZulu-Natal. Retrieved from https://researchspace.ukzn.ac.za/handle/10413/17063

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mathew, Isack. “Pre-breeding of wheat (Triticum aestivum L.) for Biomass allocation and drought tolerance.” 2018. Thesis, University of KwaZulu-Natal. Accessed January 19, 2021. https://researchspace.ukzn.ac.za/handle/10413/17063.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mathew, Isack. “Pre-breeding of wheat (Triticum aestivum L.) for Biomass allocation and drought tolerance.” 2018. Web. 19 Jan 2021.

Vancouver:

Mathew I. Pre-breeding of wheat (Triticum aestivum L.) for Biomass allocation and drought tolerance. [Internet] [Thesis]. University of KwaZulu-Natal; 2018. [cited 2021 Jan 19]. Available from: https://researchspace.ukzn.ac.za/handle/10413/17063.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mathew I. Pre-breeding of wheat (Triticum aestivum L.) for Biomass allocation and drought tolerance. [Thesis]. University of KwaZulu-Natal; 2018. Available from: https://researchspace.ukzn.ac.za/handle/10413/17063

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Ghana

14. Darkwah. Genetic Variability in Dura Oil Palm (Elaeis Guineensis Jacq) Germplasm Collected In Ghana .

Degree: 2018, University of Ghana

URL: http://ugspace.ug.edu.gh/handle/123456789/27490

► The success of any breeding programme depends to a large extent on the amount of genetic variability present in the working population at the disposal… (more)

▼ The success of any breeding programme depends to a large extent on the amount of genetic variability present in the working population at the disposal of the breeder(s). An experiment was set out to assess the genetic variability among 79 accessions of natural dura oil palm collected in Ghana and conserved at OPRI by evaluating 18 agro-morphological traits, 11 years after field planting. The 79 dura oil palms accessions were laid out in an augmented design with two checks on each block. Data were collected on vegetative, yield and yield related characters (bunch and fruit characters). Collected data were analysed using R-statistical software. Results revealed significant variation among the 79 dura oil palm accessions for all traits except total fronds, average bunch weight, percent fruit to bunch and percent kernel to fruit. Heritability (h2), ranged from 0 to 85.27 %, genetic coefficient of variation (GCV) from 0 to 68.74%, phenotypic coefficient of variation (PCV), from 7.74 to 95.76% and genetic advance (GA), from 0 to 12.72 %. Fresh fruit bunch and bunch weight exhibited high values for h2, GCV and moderate GA suggesting a high potential for improvement through selection and better response to selection. Five axes were identified using the principal components (PCs) with Eigen values greater than one explaining 74.82% of the total variance. The first to fifth components accounted for 34.25%, 15.10%, 11.86%, 7.47% and 6.13% of the variation respectively. Accessions GHA 217/02, GHA 221/02, GHA 225/03, GHA 212/02, GHA 203/02, GHA 201/01, GHA 202/01, GHA 221/04, GHA 209/02, GHA 202/02 and GHA 211/02 were phenotypically distant (more diverse) and could be selected for the improvement of the oil palm. Cluster analysis also resolved the accessions into two main clusters based on the 18 agro-morphological traits at 98 % dissimilarity index. Six pairs of accessions were identified as potential duplicates. There was no correlation between clustering and location of collection. Generally, significant positive correlations were found among the vegetative and yield characters while negative correlations were found between vegetative and yield traits on one hand and bunch and fruit characters on the other hand. There was a perfect association between the fresh fruit bunch and bunch weight. Potential accessions were identified and have been recommended for utilization in future improvement of the crop. The results suggests potential for effective utilisation of the dura collections in the OPRI’s oil palm breeding programmes and the possibility of establishing a core collections of dura oil palm from the germplasm collection evaluated in this work.

Subjects/Keywords: Genetic; Variability; Oil Palm; Germplasm; Heritability; Coefficient

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Darkwah. (2018). Genetic Variability in Dura Oil Palm (Elaeis Guineensis Jacq) Germplasm Collected In Ghana . (Masters Thesis). University of Ghana. Retrieved from http://ugspace.ug.edu.gh/handle/123456789/27490

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Chicago Manual of Style (16^th Edition):

Darkwah. “Genetic Variability in Dura Oil Palm (Elaeis Guineensis Jacq) Germplasm Collected In Ghana .” 2018. Masters Thesis, University of Ghana. Accessed January 19, 2021. http://ugspace.ug.edu.gh/handle/123456789/27490.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

MLA Handbook (7^th Edition):

Darkwah. “Genetic Variability in Dura Oil Palm (Elaeis Guineensis Jacq) Germplasm Collected In Ghana .” 2018. Web. 19 Jan 2021.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

Darkwah. Genetic Variability in Dura Oil Palm (Elaeis Guineensis Jacq) Germplasm Collected In Ghana . [Internet] [Masters thesis]. University of Ghana; 2018. [cited 2021 Jan 19]. Available from: http://ugspace.ug.edu.gh/handle/123456789/27490.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Council of Science Editors:

Darkwah. Genetic Variability in Dura Oil Palm (Elaeis Guineensis Jacq) Germplasm Collected In Ghana . [Masters Thesis]. University of Ghana; 2018. Available from: http://ugspace.ug.edu.gh/handle/123456789/27490

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

University of Aberdeen

15. Edwards, Catriona Helen. Drug target identification in the cat flea by transcriptomics and gene knockdown.

Degree: PhD, 2018, University of Aberdeen

URL: https://eu03.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12153018080005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.742385

► Ctenocephalides felis is a major pest of companion animals worldwide. This project aimed to generate novel genetic resources for C. felis and develop tools to… (more)

▼ Ctenocephalides felis is a major pest of companion animals worldwide. This project aimed to generate novel genetic resources for C. felis and develop tools to aid drug-target identification and validation. Sample handling methods were assessed and candidate reference genes validated, to ensure quality of RNA samples and reliable gene expression normalisation. Piercing C. felis samples prior to storage in RNAlater ensured RNA integrity was maintained over time. Glyceraldehyde 3-phosphate dehydrogenase , 60S ribosomal protein L19 and elongation factor-1α were demonstrated as stable reference genes across all comparisons tested. A C. felis transcriptome encompassing multiple developmental stages, sexes and tissues was sequenced and de novo assemblies produced with two assemblers, Trinity and Oases. Each assembly contained >100000 contigs. Annotation of the assemblies generated functional insight, such as top BLAST hits, GO annotations and signal peptide predictions. The Trinity assembly was deemed the highest quality and searched for genes of interest, involved in development. Expression analysis of selected transcripts across stadia gave insight into developmental processes, and demonstrated the utility of the transcriptome. This study was the first to demonstrate that C. felis can mount an RNAi response upon exposure to dsRNA. Knockdown of glutathione S-transferase σ (GSTσ), was demonstrated in adult C. felis: ≈80 % knockdown following microinjection of dsGSTσ; ≈64 % knockdown after soaking in dsGSTσ; ≈96 % knockdown after continuous feeding on dsGSTσ. RNAi machinery was identified in C. felis. siRNAi pathway components, Dicer 2 and Argonaute 2, were upregulated following dsRNA exposure. Dicer 2 was knocked-down by soaking in dsDicer2, although results of an “RNAi of RNAi” experiment were inconclusive. Transcripts encoding machinery putatively involved in dsRNA uptake and breakdown were also identified. Through these studies, this project has generated novel insights into C. felis biology and opened up new avenues for research.

Subjects/Keywords: 590; Cat flea; Germplasm resources; RNA interference

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APA (6^th Edition):

Edwards, C. H. (2018). Drug target identification in the cat flea by transcriptomics and gene knockdown. (Doctoral Dissertation). University of Aberdeen. Retrieved from https://eu03.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12153018080005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.742385

Chicago Manual of Style (16^th Edition):

Edwards, Catriona Helen. “Drug target identification in the cat flea by transcriptomics and gene knockdown.” 2018. Doctoral Dissertation, University of Aberdeen. Accessed January 19, 2021. https://eu03.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12153018080005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.742385.

MLA Handbook (7^th Edition):

Edwards, Catriona Helen. “Drug target identification in the cat flea by transcriptomics and gene knockdown.” 2018. Web. 19 Jan 2021.

Vancouver:

Edwards CH. Drug target identification in the cat flea by transcriptomics and gene knockdown. [Internet] [Doctoral dissertation]. University of Aberdeen; 2018. [cited 2021 Jan 19]. Available from: https://eu03.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12153018080005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.742385.

Council of Science Editors:

Edwards CH. Drug target identification in the cat flea by transcriptomics and gene knockdown. [Doctoral Dissertation]. University of Aberdeen; 2018. Available from: https://eu03.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12153018080005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.742385

University of Minnesota

16. Ollhoff, Alexandrea. Development of a multiparent population for genetic mapping and allele discovery in six-row barley.

Degree: MS, Applied Plant Sciences, 2018, University of Minnesota

URL: http://hdl.handle.net/11299/198954

► Germplasm collections hold valuable allelic diversity for crop improvement and genetic mapping of complex traits. To gain access to the genetic diversity within the USDA… (more)

Subjects/Keywords: Allelic diversity; Germplasm; Multi parent population; NAM

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ollhoff, A. (2018). Development of a multiparent population for genetic mapping and allele discovery in six-row barley. (Masters Thesis). University of Minnesota. Retrieved from http://hdl.handle.net/11299/198954

Chicago Manual of Style (16^th Edition):

Ollhoff, Alexandrea. “Development of a multiparent population for genetic mapping and allele discovery in six-row barley.” 2018. Masters Thesis, University of Minnesota. Accessed January 19, 2021. http://hdl.handle.net/11299/198954.

MLA Handbook (7^th Edition):

Ollhoff, Alexandrea. “Development of a multiparent population for genetic mapping and allele discovery in six-row barley.” 2018. Web. 19 Jan 2021.

Vancouver:

Ollhoff A. Development of a multiparent population for genetic mapping and allele discovery in six-row barley. [Internet] [Masters thesis]. University of Minnesota; 2018. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/11299/198954.

Council of Science Editors:

Ollhoff A. Development of a multiparent population for genetic mapping and allele discovery in six-row barley. [Masters Thesis]. University of Minnesota; 2018. Available from: http://hdl.handle.net/11299/198954

University of Hawaii – Manoa

17. Hodgin, Heather Mariel. Safeguarding Anthurium genetic resources : in vitro germplasm bank development and flow cytometry analysis.

Degree: MS, 2011, University of Hawaii – Manoa

URL: http://hdl.handle.net/10125/20917

►

vi, 41 leaves, bound col. ill. 29 cm

This thesis is a compilation, as chapters, of four individual documents under the scope of safeguarding Anthurium… (more)

Subjects/Keywords: Anthuriums – Germplasm resources; Gene banks, Plant

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hodgin, H. M. (2011). Safeguarding Anthurium genetic resources : in vitro germplasm bank development and flow cytometry analysis. (Masters Thesis). University of Hawaii – Manoa. Retrieved from http://hdl.handle.net/10125/20917

Chicago Manual of Style (16^th Edition):

Hodgin, Heather Mariel. “Safeguarding Anthurium genetic resources : in vitro germplasm bank development and flow cytometry analysis.” 2011. Masters Thesis, University of Hawaii – Manoa. Accessed January 19, 2021. http://hdl.handle.net/10125/20917.

MLA Handbook (7^th Edition):

Hodgin, Heather Mariel. “Safeguarding Anthurium genetic resources : in vitro germplasm bank development and flow cytometry analysis.” 2011. Web. 19 Jan 2021.

Vancouver:

Hodgin HM. Safeguarding Anthurium genetic resources : in vitro germplasm bank development and flow cytometry analysis. [Internet] [Masters thesis]. University of Hawaii – Manoa; 2011. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10125/20917.

Council of Science Editors:

Hodgin HM. Safeguarding Anthurium genetic resources : in vitro germplasm bank development and flow cytometry analysis. [Masters Thesis]. University of Hawaii – Manoa; 2011. Available from: http://hdl.handle.net/10125/20917

University of Guelph

18. Jaroenchai, Chutinan. Potential of Long-Ear Genetic Background to Improve Short-Season Maize Hybrids.

Degree: PhD, Department of Plant Agriculture, 2017, University of Guelph

URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/10866

► Short-season maize hybrids may be improved by the introgression of novel germplasm containing useful alleles for enhancing grain yield. This thesis investigated the potential of… (more)

Subjects/Keywords: short-season maize; maize improvement; exotic germplasm; BSLE(M-L)C30; long ear germplasm

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jaroenchai, C. (2017). Potential of Long-Ear Genetic Background to Improve Short-Season Maize Hybrids. (Doctoral Dissertation). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/10866

Chicago Manual of Style (16^th Edition):

Jaroenchai, Chutinan. “Potential of Long-Ear Genetic Background to Improve Short-Season Maize Hybrids.” 2017. Doctoral Dissertation, University of Guelph. Accessed January 19, 2021. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/10866.

MLA Handbook (7^th Edition):

Jaroenchai, Chutinan. “Potential of Long-Ear Genetic Background to Improve Short-Season Maize Hybrids.” 2017. Web. 19 Jan 2021.

Vancouver:

Jaroenchai C. Potential of Long-Ear Genetic Background to Improve Short-Season Maize Hybrids. [Internet] [Doctoral dissertation]. University of Guelph; 2017. [cited 2021 Jan 19]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/10866.

Council of Science Editors:

Jaroenchai C. Potential of Long-Ear Genetic Background to Improve Short-Season Maize Hybrids. [Doctoral Dissertation]. University of Guelph; 2017. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/10866

19. Teerawat Sarutayophat. Evaluation of germplasm and comparison between pedigree and single descent methods in Yardlong bean (vigna unguiculata ssp. sesquipedalis (L.) Verde) .

Degree: คณะทรัพยากรธรรมชาติ ภาควิชาพืชศาสตร์, 2008, Prince of Songkla University

URL: http://kb.psu.ac.th/psukb/handle/2010/5871

Subjects/Keywords: Beans Germplasm resources; Yardlong bean Germplasm resources; Germplasm resources, Plant

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sarutayophat, T. (2008). Evaluation of germplasm and comparison between pedigree and single descent methods in Yardlong bean (vigna unguiculata ssp. sesquipedalis (L.) Verde) . (Thesis). Prince of Songkla University. Retrieved from http://kb.psu.ac.th/psukb/handle/2010/5871

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sarutayophat, Teerawat. “Evaluation of germplasm and comparison between pedigree and single descent methods in Yardlong bean (vigna unguiculata ssp. sesquipedalis (L.) Verde) .” 2008. Thesis, Prince of Songkla University. Accessed January 19, 2021. http://kb.psu.ac.th/psukb/handle/2010/5871.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sarutayophat, Teerawat. “Evaluation of germplasm and comparison between pedigree and single descent methods in Yardlong bean (vigna unguiculata ssp. sesquipedalis (L.) Verde) .” 2008. Web. 19 Jan 2021.

Vancouver:

Sarutayophat T. Evaluation of germplasm and comparison between pedigree and single descent methods in Yardlong bean (vigna unguiculata ssp. sesquipedalis (L.) Verde) . [Internet] [Thesis]. Prince of Songkla University; 2008. [cited 2021 Jan 19]. Available from: http://kb.psu.ac.th/psukb/handle/2010/5871.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sarutayophat T. Evaluation of germplasm and comparison between pedigree and single descent methods in Yardlong bean (vigna unguiculata ssp. sesquipedalis (L.) Verde) . [Thesis]. Prince of Songkla University; 2008. Available from: http://kb.psu.ac.th/psukb/handle/2010/5871

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Johannesburg

20. Chatiza, Fungayi Primrose. Characterization of testes and functional evaluation of cryopreserved epididymal spermatozoa from three South African antelope species.

Degree: 2014, University of Johannesburg

URL: http://hdl.handle.net/10210/8791

►

Ph.D. (Zoology)

This project involves a detailed study of three South African antelope species, springbok, impala and blesbok. The study investigates the origins of sperm… (more)

Subjects/Keywords: Cryopreservation of organs, tissues, etc. - Methodology; Blesbok - Germplasm resources - Cryopreservation - South Africa; Impala - Germplasm resources - Cryopreservation - South Africa; Springbok - Germplasm resources - Cryopreservation - South Africa; Game protection - South Africa

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chatiza, F. P. (2014). Characterization of testes and functional evaluation of cryopreserved epididymal spermatozoa from three South African antelope species. (Thesis). University of Johannesburg. Retrieved from http://hdl.handle.net/10210/8791

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chatiza, Fungayi Primrose. “Characterization of testes and functional evaluation of cryopreserved epididymal spermatozoa from three South African antelope species.” 2014. Thesis, University of Johannesburg. Accessed January 19, 2021. http://hdl.handle.net/10210/8791.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chatiza, Fungayi Primrose. “Characterization of testes and functional evaluation of cryopreserved epididymal spermatozoa from three South African antelope species.” 2014. Web. 19 Jan 2021.

Vancouver:

Chatiza FP. Characterization of testes and functional evaluation of cryopreserved epididymal spermatozoa from three South African antelope species. [Internet] [Thesis]. University of Johannesburg; 2014. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10210/8791.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chatiza FP. Characterization of testes and functional evaluation of cryopreserved epididymal spermatozoa from three South African antelope species. [Thesis]. University of Johannesburg; 2014. Available from: http://hdl.handle.net/10210/8791

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Durban University of Technology

21. Makhathini, Aneliswa Phumzile. Plant germplasm conservation :|bdevelopment of field collection and transport techniques for eucalyptus species and trichilia dregeana.

Degree: 2002, Durban University of Technology

URL: http://hdl.handle.net/10321/2063

►

Dissertation submitted in compliance with the requirements for the Master's Degree in Technology: Biotechnology, Technikon Natal, 2002.

Lack of suitable techniques for field collection of… (more)

▼

Dissertation submitted in compliance with the requirements for the Master's Degree in Technology: Biotechnology, Technikon Natal, 2002.

Lack of suitable techniques for field collection of the germplasm of different species, and spoilage of samples in transit, hinder efforts to collect, conserve, distribute and regenerate most plant germplasm in vitro. The aims of this investigation, therefore, were to address problems encountered in collection of field germplasm from species and hybrids of Eucalyptus (TAG5, TAGI4, ZG14, GC550 and GU2IO) that are propagated vegetatively and Trichilia dregeana, which has recalcitrant seeds. Simple in vitro culture-based protocols were developed to minimise contamination and maintain viability of plant material for sufficient time for it to be transported from the field to the tissue culture laboratory. From the two simulations of 48 h 'transportation' conditions for explants of Eucalyptus species investigated, those in bottles containing sterile vermiculite exhibited no contamination and greater than 50% bud break, regardless of whether or not field surface sterilization with alcohol had been done. In contrast, when explants were enclosed in cling wrap, contamination was high and bud break levels low. For selection of the more suitable Eucalyptus explant, two types were investigated: nodal explants each with one half leaf (type 1) and stem segments with three nodes (type 2). As type 2 showed considerably better shoot yields (up to 1624 shoots per 100 explant), and were more practical to use with respect to space, such trinodal stem segments were deemed best for collection. Of the sterilization procedures investigated, treatment with 70% (v/v) alcohol prior to storage was found to be most suitable in almost all cases. For plant material with high endogenous microbial contamination, the bud break medium was supplemented with Benomyl and calcium hypochlorite, each at 0.5 and 1.0 g.r). Alcoholtreated, stored explants cultured on bud break medium with 1.0 g.r) calcium hypochlorite exhibited low levels of contamination and an increased final yield (up to a maximum of 930 shoots per 100 explants). Thus, this protocol was employed for field material of E. grandis clones TAG5, TAGI4 and ZGI4. For these clones, stored type 2 explants

M

Advisors/Committee Members: Watt, Maria Paula.

Subjects/Keywords: Eucalyptus – Biotechnology; Germplasm resources conservation; Germplasm resources, Plant

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Makhathini, A. P. (2002). Plant germplasm conservation :|bdevelopment of field collection and transport techniques for eucalyptus species and trichilia dregeana. (Thesis). Durban University of Technology. Retrieved from http://hdl.handle.net/10321/2063

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Makhathini, Aneliswa Phumzile. “Plant germplasm conservation :|bdevelopment of field collection and transport techniques for eucalyptus species and trichilia dregeana.” 2002. Thesis, Durban University of Technology. Accessed January 19, 2021. http://hdl.handle.net/10321/2063.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Makhathini, Aneliswa Phumzile. “Plant germplasm conservation :|bdevelopment of field collection and transport techniques for eucalyptus species and trichilia dregeana.” 2002. Web. 19 Jan 2021.

Vancouver:

Makhathini AP. Plant germplasm conservation :|bdevelopment of field collection and transport techniques for eucalyptus species and trichilia dregeana. [Internet] [Thesis]. Durban University of Technology; 2002. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10321/2063.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Makhathini AP. Plant germplasm conservation :|bdevelopment of field collection and transport techniques for eucalyptus species and trichilia dregeana. [Thesis]. Durban University of Technology; 2002. Available from: http://hdl.handle.net/10321/2063

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Michigan State University

22. Krahl, Kimberly Howell. Principal component analysis and isozyme analysis of sweet, sour, and ground cherry germplasm.

Degree: MS, Department of Horticulture, 1989, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:20710

Subjects/Keywords: Cherry – Germplasm resources; Germplasm resources, Plant; Isoenzymes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Krahl, K. H. (1989). Principal component analysis and isozyme analysis of sweet, sour, and ground cherry germplasm. (Masters Thesis). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:20710

Chicago Manual of Style (16^th Edition):

Krahl, Kimberly Howell. “Principal component analysis and isozyme analysis of sweet, sour, and ground cherry germplasm.” 1989. Masters Thesis, Michigan State University. Accessed January 19, 2021. http://etd.lib.msu.edu/islandora/object/etd:20710.

MLA Handbook (7^th Edition):

Krahl, Kimberly Howell. “Principal component analysis and isozyme analysis of sweet, sour, and ground cherry germplasm.” 1989. Web. 19 Jan 2021.

Vancouver:

Krahl KH. Principal component analysis and isozyme analysis of sweet, sour, and ground cherry germplasm. [Internet] [Masters thesis]. Michigan State University; 1989. [cited 2021 Jan 19]. Available from: http://etd.lib.msu.edu/islandora/object/etd:20710.

Council of Science Editors:

Krahl KH. Principal component analysis and isozyme analysis of sweet, sour, and ground cherry germplasm. [Masters Thesis]. Michigan State University; 1989. Available from: http://etd.lib.msu.edu/islandora/object/etd:20710

University of Pretoria

23. [No author]. Increasing line combining ability and gray leaf spot resistance in maize by integrating conventional with DNA marker technology .

Degree: 2008, University of Pretoria

URL: http://upetd.up.ac.za/thesis/available/etd-07282008-134757/

► Maize is the staple food for the majority of Tanzanians. However, maize production in the Southern highlands of Tanzania (SHT) is highly reduced by gray… (more)

▼ Maize is the staple food for the majority of Tanzanians. However, maize production in the Southern highlands of Tanzania (SHT) is highly reduced by gray leaf spot disease (GLS) caused by the fungus Cercospora zea maydis. GLS reduces grain yield, kernel and silage quality. The most common GLS control methods in Tanzania include amongst others; fungicides, crop rotation, field sanitation, host resistance. These methods except host resistance are, however, either expensive or less effective or unsafe to the environment. Furthermore, conventional breeding strategies are not very effective for traits, which are lowly inherited such as GLS resistance. Lastly, to date there are few GLS resistant commercial hybrids in SHT. Thus, this study aimed to produce more commercial GLS resistant hybrids, increase farmers’ hybrid choices of growing genetically different GLS insensitive hybrids, which will also provide a constant supply of GLS resistant maize cultivars in case of GLS resistance breakdown due to new GLS pathotypes. This research combined conventional breeding with molecular technologies to increase the efficacy of selecting GLS resistant hybrids and assist breeders in predicting best inbred combinations for commercial hybrid production. Studies conducted to meet the main aims were on; the prediction of best line combiners and heterosis in Tanzanian maize breeding lines through the use of amplified fragment length polymorphism, (AFLP), an association of AFLPs and the performance of phenotypic traits in maize, evaluation of maize hybrids for gray leaf spot resistance in multienvironments and finally a preliminary study on gray leaf spot PCR-based marker development with the long term objective of implementing cleaved amplified polymorphic markers (CAPS) in a marker assisted selection (MAS) strategy in the SHT maize breeding programme. Results from the study revealed that pairwise GD (genetic distance) of the lines varied from a GD of 0.13 to 0.5. High coancentry coefficients were exhibited by these lines. Joint data analyses showed that there were tighter associations between line GD and F1 traits or MPH in the intergroup than in the intragroup crosses. Combined analyses revealed that hybrids 48, 90 and 45 recorded higher stable yields and consistently low GLS scores in multienvironments. Fifteen CAPS marker bands were identified that are putatively linked to the GLS resistant genes. In summary, it was noted that strong selection during inbreeding programs should be avoided as it reduces germplasm variability. Local landraces/varieties can be improved by introgressing desirable genes into them. AFLP marker system could be effectively used for inbred genetic diversity studies in Tanzania. Intergroup crosses with high GD-MPH should be the main target for commercial hybrid production but field testing of them is inevitable to confirm their yielding potentials. Intergroups and intragroup crosses with low GD-MPH should be discarded to avoid field costs. Better F1 hybrid performance predictions can be achieved by integrating… Advisors/Committee Members: Dr N G Lyimo (advisor), Prof A-M Botha-Oberholster (advisor).

Subjects/Keywords: Gls; Aflp; Germplasm; Genetic diversity; Gd; Dendrogram; UCTD

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

author], [. (2008). Increasing line combining ability and gray leaf spot resistance in maize by integrating conventional with DNA marker technology . (Doctoral Dissertation). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-07282008-134757/

Chicago Manual of Style (16^th Edition):

author], [No. “Increasing line combining ability and gray leaf spot resistance in maize by integrating conventional with DNA marker technology .” 2008. Doctoral Dissertation, University of Pretoria. Accessed January 19, 2021. http://upetd.up.ac.za/thesis/available/etd-07282008-134757/.

MLA Handbook (7^th Edition):

author], [No. “Increasing line combining ability and gray leaf spot resistance in maize by integrating conventional with DNA marker technology .” 2008. Web. 19 Jan 2021.

Vancouver:

author] [. Increasing line combining ability and gray leaf spot resistance in maize by integrating conventional with DNA marker technology . [Internet] [Doctoral dissertation]. University of Pretoria; 2008. [cited 2021 Jan 19]. Available from: http://upetd.up.ac.za/thesis/available/etd-07282008-134757/.

Council of Science Editors:

author] [. Increasing line combining ability and gray leaf spot resistance in maize by integrating conventional with DNA marker technology . [Doctoral Dissertation]. University of Pretoria; 2008. Available from: http://upetd.up.ac.za/thesis/available/etd-07282008-134757/

University of California – Riverside

24. Lucas, Mitchell Ryan. Using Genomic Resources to Breed Cowpeas With Larger Seeds.

Degree: Genetics, Genomics and Bioinformatics, 2014, University of California – Riverside

URL: http://www.escholarship.org/uc/item/9m50q1w2

► Cowpea (Vigna unguiculata) is a warm-season legume that is primarily cultivated for protein rich grain. Seed size is an important breeding target that distinguishes most… (more)

Subjects/Keywords: Genetics; Plant sciences; Breeding; Cowpea; Genomics; Germplasm; SNPs

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lucas, M. R. (2014). Using Genomic Resources to Breed Cowpeas With Larger Seeds. (Thesis). University of California – Riverside. Retrieved from http://www.escholarship.org/uc/item/9m50q1w2

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lucas, Mitchell Ryan. “Using Genomic Resources to Breed Cowpeas With Larger Seeds.” 2014. Thesis, University of California – Riverside. Accessed January 19, 2021. http://www.escholarship.org/uc/item/9m50q1w2.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lucas, Mitchell Ryan. “Using Genomic Resources to Breed Cowpeas With Larger Seeds.” 2014. Web. 19 Jan 2021.

Vancouver:

Lucas MR. Using Genomic Resources to Breed Cowpeas With Larger Seeds. [Internet] [Thesis]. University of California – Riverside; 2014. [cited 2021 Jan 19]. Available from: http://www.escholarship.org/uc/item/9m50q1w2.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lucas MR. Using Genomic Resources to Breed Cowpeas With Larger Seeds. [Thesis]. University of California – Riverside; 2014. Available from: http://www.escholarship.org/uc/item/9m50q1w2

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Pretoria

25. Kiula, Barnabas Anthony. Increasing line combining ability and gray leaf spot resistance in maize by integrating conventional with DNA marker technology.

Degree: Genetics, 2008, University of Pretoria

URL: http://hdl.handle.net/2263/26756

► Maize is the staple food for the majority of Tanzanians. However, maize production in the Southern highlands of Tanzania (SHT) is highly reduced by gray… (more)

▼ Maize is the staple food for the majority of Tanzanians. However, maize production in the Southern highlands of Tanzania (SHT) is highly reduced by gray leaf spot disease (GLS) caused by the fungus Cercospora zea maydis. GLS reduces grain yield, kernel and silage quality. The most common GLS control methods in Tanzania include amongst others; fungicides, crop rotation, field sanitation, host resistance. These methods except host resistance are, however, either expensive or less effective or unsafe to the environment. Furthermore, conventional breeding strategies are not very effective for traits, which are lowly inherited such as GLS resistance. Lastly, to date there are few GLS resistant commercial hybrids in SHT. Thus, this study aimed to produce more commercial GLS resistant hybrids, increase farmers’ hybrid choices of growing genetically different GLS insensitive hybrids, which will also provide a constant supply of GLS resistant maize cultivars in case of GLS resistance breakdown due to new GLS pathotypes. This research combined conventional breeding with molecular technologies to increase the efficacy of selecting GLS resistant hybrids and assist breeders in predicting best inbred combinations for commercial hybrid production. Studies conducted to meet the main aims were on; the prediction of best line combiners and heterosis in Tanzanian maize breeding lines through the use of amplified fragment length polymorphism, (AFLP), an association of AFLPs and the performance of phenotypic traits in maize, evaluation of maize hybrids for gray leaf spot resistance in multienvironments and finally a preliminary study on gray leaf spot PCR-based marker development with the long term objective of implementing cleaved amplified polymorphic markers (CAPS) in a marker assisted selection (MAS) strategy in the SHT maize breeding programme. Results from the study revealed that pairwise GD (genetic distance) of the lines varied from a GD of 0.13 to 0.5. High coancentry coefficients were exhibited by these lines. Joint data analyses showed that there were tighter associations between line GD and F1 traits or MPH in the intergroup than in the intragroup crosses. Combined analyses revealed that hybrids 48, 90 and 45 recorded higher stable yields and consistently low GLS scores in multienvironments. Fifteen CAPS marker bands were identified that are putatively linked to the GLS resistant genes. In summary, it was noted that strong selection during inbreeding programs should be avoided as it reduces germplasm variability. Local landraces/varieties can be improved by introgressing desirable genes into them. AFLP marker system could be effectively used for inbred genetic diversity studies in Tanzania. Intergroup crosses with high GD-MPH should be the main target for commercial hybrid production but field testing of them is inevitable to confirm their yielding potentials. Intergroups and intragroup crosses with low GD-MPH should be discarded to avoid field costs. Better F1 hybrid performance predictions can be achieved by integrating… Advisors/Committee Members: Dr N G Lyimo (advisor), Prof A-M Botha-Oberholster (advisor).

Subjects/Keywords: Gls; Aflp; Germplasm; Genetic diversity; Gd; Dendrogram; UCTD

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kiula, B. (2008). Increasing line combining ability and gray leaf spot resistance in maize by integrating conventional with DNA marker technology. (Doctoral Dissertation). University of Pretoria. Retrieved from http://hdl.handle.net/2263/26756

Chicago Manual of Style (16^th Edition):

Kiula, Barnabas. “Increasing line combining ability and gray leaf spot resistance in maize by integrating conventional with DNA marker technology.” 2008. Doctoral Dissertation, University of Pretoria. Accessed January 19, 2021. http://hdl.handle.net/2263/26756.

MLA Handbook (7^th Edition):

Kiula, Barnabas. “Increasing line combining ability and gray leaf spot resistance in maize by integrating conventional with DNA marker technology.” 2008. Web. 19 Jan 2021.

Vancouver:

Kiula B. Increasing line combining ability and gray leaf spot resistance in maize by integrating conventional with DNA marker technology. [Internet] [Doctoral dissertation]. University of Pretoria; 2008. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/2263/26756.

Council of Science Editors:

Kiula B. Increasing line combining ability and gray leaf spot resistance in maize by integrating conventional with DNA marker technology. [Doctoral Dissertation]. University of Pretoria; 2008. Available from: http://hdl.handle.net/2263/26756

Oregon State University

26. Luo, Jie, 1968-. Improvement of cryopreservation regrowth of in vitro Ribes by pretreatment.

Degree: MS, Horticulture, 1996, Oregon State University

URL: http://hdl.handle.net/1957/25686

► Improved recovery of cryopreserved meristems and calli from in vitro currant (Ribes aureum Pursh and R. ciliatum Humb. & Bonpl.) plants was obtained by two-hour… (more)

Subjects/Keywords: Ribes – Germplasm resources – Cryopreservation

Record Details Similar Records Cite « Share

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APA (6^th Edition):

Luo, Jie, 1. (1996). Improvement of cryopreservation regrowth of in vitro Ribes by pretreatment. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/25686

Chicago Manual of Style (16^th Edition):

Luo, Jie, 1968-. “Improvement of cryopreservation regrowth of in vitro Ribes by pretreatment.” 1996. Masters Thesis, Oregon State University. Accessed January 19, 2021. http://hdl.handle.net/1957/25686.

MLA Handbook (7^th Edition):

Luo, Jie, 1968-. “Improvement of cryopreservation regrowth of in vitro Ribes by pretreatment.” 1996. Web. 19 Jan 2021.

Vancouver:

Luo, Jie 1. Improvement of cryopreservation regrowth of in vitro Ribes by pretreatment. [Internet] [Masters thesis]. Oregon State University; 1996. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1957/25686.

Council of Science Editors:

Luo, Jie 1. Improvement of cryopreservation regrowth of in vitro Ribes by pretreatment. [Masters Thesis]. Oregon State University; 1996. Available from: http://hdl.handle.net/1957/25686

27. Thankappan, Smitha S. Micropropagation techniques, germplasm conservation and genetic transformation studies in Dioscorea prazeri; -.

Degree: Biotechnology, 2014, University of Mysore

URL: http://shodhganga.inflibnet.ac.in/handle/10603/15954

►

Dioscorea prazeri is a valuable medicinal plant belongs to the genera of yams, found newlinechiefly at higher altitudes of North Eastern Himalayas. Diosgenin, the therapeutically… (more)

▼

Dioscorea prazeri is a valuable medicinal plant belongs to the genera of yams, found newlinechiefly at higher altitudes of North Eastern Himalayas. Diosgenin, the therapeutically newlinesignificant secondary metabolite present in D. prazeri tubers is amongst the 10 most newlineimportant sources of steroids, widely prescribed medicine of plant origin. This research newlineholds immense potential in exploring the bioactive, Diosgenin and sustainable newlinemanagement and utilization of this valuable medicinal yam, threatened and indigenous to newlineIndia. A highly efficient in vitro regeneration of D. prazeri was achieved using nodal newlineexplants and axillary buds on Murashige and Skoog medium containing sucrose, newlinesupplemented with growth regulators. Germplasm conservation technique was newlineestablished using vitrification and encapsulation dehydration method and a combination newlineof these two. This protocol ensures long-term availability of this precious germplasm and newlinecan be successfully exploited for conservation of Dioscorea species and other elite plant newlinespecies. The plantlets had healthy roots and sprouted tubers in vitro, were subsequently newlineacclimatized and successfully established in soil. The regenerated D. prazeri in this study newlinewould be reintroduced to the natural habitat. The method of extraction and newlinechromatographic analysis were standardized to obtain maximum yield of Diosgenin from newlineextracts of D. prazeri. An examination of the genetic fidelity of the in vitro regenerated newlineplants using morphological, molecular and biochemical methods indicated a stable newlinegenome. The gene of interest, 3-hydroxy-3-methyl-glutaryl-CoA reductase 1, was isolated newlinefrom Hevea brasiliensis for genetic transformation studies on D. prazeri. The RNA was newlinereverse transcribed and amplified with gene specific primers to obtain the 1.8 kb newlinetranscript. The sequence of confirmed gene was further genetically engineered into plant newlineexpression vector, introduced into D. prazeri via A. tumefaciens mediated transformation.

Bibliography and Appendix included

Advisors/Committee Members: Villoo Morawala Patell.

Subjects/Keywords: Biotechnology; germplasm conservation; genetic transformation studies; Dioscorea prazeri

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Thankappan, S. S. (2014). Micropropagation techniques, germplasm conservation and genetic transformation studies in Dioscorea prazeri; -. (Thesis). University of Mysore. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/15954

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Thankappan, Smitha S. “Micropropagation techniques, germplasm conservation and genetic transformation studies in Dioscorea prazeri; -.” 2014. Thesis, University of Mysore. Accessed January 19, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/15954.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Thankappan, Smitha S. “Micropropagation techniques, germplasm conservation and genetic transformation studies in Dioscorea prazeri; -.” 2014. Web. 19 Jan 2021.

Vancouver:

Thankappan SS. Micropropagation techniques, germplasm conservation and genetic transformation studies in Dioscorea prazeri; -. [Internet] [Thesis]. University of Mysore; 2014. [cited 2021 Jan 19]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/15954.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Thankappan SS. Micropropagation techniques, germplasm conservation and genetic transformation studies in Dioscorea prazeri; -. [Thesis]. University of Mysore; 2014. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/15954

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Federal de Viçosa

28. Cristiane Gamarano de Melo. Criopreservação de germoplasma de cana-de-açúcar.

Degree: 2008, Universidade Federal de Viçosa

URL: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=1873

►

O objetivo deste trabalho foi o desenvolvimento de protocolos para criopreservação, em nitrogênio líquido a -196C, de germoplasma de cana-de-açúcar. O tempo de exposição dos… (more)

▼

O objetivo deste trabalho foi o desenvolvimento de protocolos para criopreservação, em nitrogênio líquido a -196C, de germoplasma de cana-de-açúcar. O tempo de exposição dos ápices caulinares encapsulados a câmara de fluxo laminar para a obtenção dos teores de umidade de 30, 20 e 10% foi determinado através de um ensaio e os resultados obtidos foram analisados e interpretados utilizando o software livre R. Os pontos médios de cada tempo avaliado foram unidos por segmentos de reta traçando uma linha de tendência de perda de umidade. Posteriormente, com o auxílio de linhas horizontais referentes aos teores de umidade de 30, 20 e 10%, os tempos de secagem foram identificados diretamente no gráfico em 5,7; 7,45 e 10,1 horas, respectivamente. Para induzir a tolerância dos ápices caulinares à secagem foi realizado o pré-cultivo em meio de cultura líquido enriquecido com 0,3; 0,5 e 0,75 M de sacarose por um e dois dias. O experimento foi organizado em um esquema fatorial 3X2X4 (concentrações de sacarose, tempo de pré-cultivo e tempo de secagem) segundo o delineamento inteiramente casualizado com três repetições. Os dados do índice de sobrevivência obtidos foram analisados e interpretados utilizando o software livre R. Foi realizada a análise de variância e quando necessário as médias foram comparadas pelo teste de Tukey a 5% de significância. Os resultados indicaram que o pré-cultivo em meio de cultura enriquecido com 0,3 M de sacarose, independentemente se por um ou dois dias, foi ideal para induzir a tolerância dos tecidos à secagem. Os ápices caulinares foram sensíveis à concentração de 0,75 M de sacarose na solução de pré- cultivo. O cultivo por 12 horas dos ápices caulinares em meio básico de cultura resultou além da recuperação do estresse gerado pela sua extração, a reativação do seu metabolismo através do acúmulo de grão de amido. A síntese de amido aumentou em intensidade quando os ápices foram pré- cultivados em meio de cultura líquido contendo sacarose nas concentrações de 0,30; 0,50 e 0,75 M. Na criopreservação dos ápices caulinares encapsulados, estudou-se o efeito de três fatores, concentração de sacarose, tempo de secagem e o método de descongelamento, em um fatorial 3X4X3. Independentemente da combinação adotada quando os ápices caulinares foram criopreservados o índice de sobrevivência obtido foi nulo. As análises histológicas revelaram que as células e a parede celular dos explantes criopreservados foram severamente danificadas.

This study was carried out to develop some protocols for cryopreservation of sugarcane germplasm in liquid nitrogen at -196C. The exposure time of the shoot tips encapsulated in the laminar flow chamber for obtaining the moisture contents of 30, 20 and 10% was determined in an assay. The results were analyzed and interpreted, by using the free R software. The medium points of each time under evaluation were united by straight line segments, as drawing a tendency line for the moisture loss. Then, with the aid of horizontal lines referring to moisture contents of 30, 20 and 10%,…

Advisors/Committee Members: Marília Contin Ventrella, Sérgio Yoshimitsu Motoike, Luiz Alexandre Peternelli, Wagner Campos Otoni, Marcio Henrique Pereira Barbosa.

Subjects/Keywords: Germoplasma; Criopreservação; Gema apical; MELHORAMENTO VEGETAL; Cryopreservation; Germplasm; Shoot tip gems

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Melo, C. G. d. (2008). Criopreservação de germoplasma de cana-de-açúcar. (Thesis). Universidade Federal de Viçosa. Retrieved from http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=1873

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Melo, Cristiane Gamarano de. “Criopreservação de germoplasma de cana-de-açúcar.” 2008. Thesis, Universidade Federal de Viçosa. Accessed January 19, 2021. http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=1873.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Melo, Cristiane Gamarano de. “Criopreservação de germoplasma de cana-de-açúcar.” 2008. Web. 19 Jan 2021.

Vancouver:

Melo CGd. Criopreservação de germoplasma de cana-de-açúcar. [Internet] [Thesis]. Universidade Federal de Viçosa; 2008. [cited 2021 Jan 19]. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=1873.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Melo CGd. Criopreservação de germoplasma de cana-de-açúcar. [Thesis]. Universidade Federal de Viçosa; 2008. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=1873

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

North Carolina State University

29. Eller, Magen Starr. Improving Resistance to Fusarium Ear Rot and Fumonisin Contamination and Increasing Yield with Exotic Maize Germplasm.

Degree: PhD, Crop Science, 2009, North Carolina State University

URL: http://www.lib.ncsu.edu/resolver/1840.16/3519

► Exotic and unadapted varieties contribute advantageous alleles to crop species. Incorporating exotic germplasm into adapted lines has the additional advantage of broadening the genetic diversity… (more)

▼ Exotic and unadapted varieties contribute advantageous alleles to crop species. Incorporating exotic germplasm into adapted lines has the additional advantage of broadening the genetic diversity within the common maize germplasm pool. I explore contributions of unadapted or exotic maize germplasm to improved resistance to Fusarium ear rot and fumonisin accumulation or topcross grain yield (quantitatively inherited traits) using different breeding approaches and population structures. Topcrosses of BC1F1:2 lines, developed by backcrossing GE440 FR1064 and selected for divergent levels of resistance to Fusarium ear rot and fumonisin contamination, were used to test the hypothesis that inbred lines with greater resistance to fumonisin contamination produce hybrids with greater ear rot resistance and greater resistance to yield loss under inoculation. Experimental results did not support the hypothesis, but this result may have been due to low levels of infection in the field trials. Selected BC4F1:3-derived lines representing advanced backcross generations of GE440 alleles into the FR1064 genetic background and their topcrossed hybrids were evaluated in field trials for disease resistance and yield potential. Experimental results demonstrate that advanced backcross generations produce lines comparable to FR1064 for grain yield but with better ear rot and fumonisin resistance, that indirect selection for reduced fumonisin content by selection for ear rot resistance was partially effective, but that selection for improved inbred disease resistance again did not result in improved topcross disease resistance. A random-mated, genetically broad-based population referred to as the Resistant to Fusarium population, was developed to combine alleles for Fusarium ear rot resistance and improved agronomic traits from diverse maize germplasm. One cycle of selection was conducted in this population to test the hypothesis that index selection for reduced Fusarium ear rot, reduced lodging, and increased yield results in reduced fumonisin contamination. Selected lines were not significantly different than the base population for ear rot percentage or fumonisin content. In topcrosses, however, selected lines showed significant improvement for ear rot resistance and fumonisin accumulation compared to the unselected Cycle 0 topcross control. Additional cycles are needed to increase favorable allele frequencies for each of the target traits. By genotyping a segregating F2:3 population from a cross between phenotypically distinct F4-derived sister lines I identified the genome region(s) conferring a topcross yield difference in a nearly-isogenic genetic background and determined that the tropical parent was the source of the favorable allele(s). Advisors/Committee Members: James B. Holland, Committee Chair (advisor), Gary A. Payne, Committee Co-Chair (advisor), Major M. Goodman, Committee Member (advisor), Gina L. Brown-Guedira, Committee Member (advisor).

Subjects/Keywords: exotic germplasm; maize; fusarium verticillioides; highly related lines; disease resistance

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Eller, M. S. (2009). Improving Resistance to Fusarium Ear Rot and Fumonisin Contamination and Increasing Yield with Exotic Maize Germplasm. (Doctoral Dissertation). North Carolina State University. Retrieved from http://www.lib.ncsu.edu/resolver/1840.16/3519

Chicago Manual of Style (16^th Edition):

Eller, Magen Starr. “Improving Resistance to Fusarium Ear Rot and Fumonisin Contamination and Increasing Yield with Exotic Maize Germplasm.” 2009. Doctoral Dissertation, North Carolina State University. Accessed January 19, 2021. http://www.lib.ncsu.edu/resolver/1840.16/3519.

MLA Handbook (7^th Edition):

Eller, Magen Starr. “Improving Resistance to Fusarium Ear Rot and Fumonisin Contamination and Increasing Yield with Exotic Maize Germplasm.” 2009. Web. 19 Jan 2021.

Vancouver:

Eller MS. Improving Resistance to Fusarium Ear Rot and Fumonisin Contamination and Increasing Yield with Exotic Maize Germplasm. [Internet] [Doctoral dissertation]. North Carolina State University; 2009. [cited 2021 Jan 19]. Available from: http://www.lib.ncsu.edu/resolver/1840.16/3519.

Council of Science Editors:

Eller MS. Improving Resistance to Fusarium Ear Rot and Fumonisin Contamination and Increasing Yield with Exotic Maize Germplasm. [Doctoral Dissertation]. North Carolina State University; 2009. Available from: http://www.lib.ncsu.edu/resolver/1840.16/3519

North Carolina State University

30. Jines, Michael P. Enhancing Genetic Gain in Maize with Tropical Germplasm, QTL Mapping, and Spatial Methodologies.

Degree: PhD, Crop Science, 2008, North Carolina State University

URL: http://www.lib.ncsu.edu/resolver/1840.16/3118

► Advance-cycle breeding is restricting the germplasm base for U.S. maize (Zea Mays L.). Many breeding programs devote efforts to adapt diverse germplasm to U.S. growing… (more)

▼ Advance-cycle breeding is restricting the germplasm base for U.S. maize (Zea Mays L.). Many breeding programs devote efforts to adapt diverse germplasm to U.S. growing conditions, but few are participating in continual enhancement. Incorporating tropical germplasm into U.S. breeding pools could broaden the maize germplasm base, while concomitantly providing favorable alleles for yield and disease resistance. Knowing the genomic regions, or quantitative trait loci (QTL), for disease resistance can enhance gain by permitting selection on marker genotypes in the absence of disease expression. In addition, accounting for spatial variability can improve the precision of experiments and aid breeders in line advancement decisions and QTL mapping. Recombinant inbred (RI) lines were derived from a cross between NC300, a temperate-adapted, all-tropical line, and B104, a Stiff-Stalk-synthetic line. The RI lines were topcrossed to the tester FR615.FR697 (a C103 sister line cross). Resistance QTL for Southern Rust (rust) (Puccinia polysora) were mapped in the topcrosses, while Gray Leaf Spot (GLS) (Cercospora zeae-maydis) QTL were mapped in both the RI lines and topcross populations. A major resistance gene for rust was identified on the short-arm of chromosome 10, while ten GLS QTL mapped to chromosomes 1, 2, 3, 4, 8, and 10. Similar markers on chromosome 1 and 8 flanked three GLS and flowering time QTL pairs, and the resistance alleles were associated with increased flowering time. No flowering time regions co-localized with rust-resistance loci. The major rust-resistance gene and three GLS QTL corresponded to regions mapped in prior populations. The tropical parental allele, NC300, increased resistance at three of these four loci. Extensively haplotyping germplasm at these four consensus regions could aid in forward breeding strategies to efficiently integrate resistance packages into U.S. maize breeding populations. Spatial analyses, such as trend and trend analysis with correlated errors models, can improve precision of genotype means estimates. These analyses often reduce the phenotypic variance among family means, and in doing so, increase the response to selection. A dynamic SAS program, entitled SPATIALPRO, was developed to implement spatial analytical techniques. The program constructs and optimizes several spatial models for each trait and single-environment-trial combination, and chooses a preferred model based on a specified criterion. Results from the preferred model are outputted into SAS data sets. A long term breeding effort was initiated in 1975 to adapt and subsequently enhance tropical germplasm. Founder germplasm included seven double-cross-tropical hybrids. Based on the poor per se performance of the first and second-cycle lines, at least five cycles of S1 recurrent selection (RS) for grain yield has been practiced on two populations derived from these lines. Cycles per se and cycle-topcrosses to LH132.LH51 were grown in separate yield trials to estimate responses to selection. In both instances, grain… Advisors/Committee Members: Major Goodman, Committee Chair (advisor), James B. Holland, Committee Member (advisor), Cavell Brownie, Committee Member (advisor), Paul Murphy, Committee Member (advisor).

Subjects/Keywords: germplasm enhancement; maize; gain

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jines, M. P. (2008). Enhancing Genetic Gain in Maize with Tropical Germplasm, QTL Mapping, and Spatial Methodologies. (Doctoral Dissertation). North Carolina State University. Retrieved from http://www.lib.ncsu.edu/resolver/1840.16/3118

Chicago Manual of Style (16^th Edition):

Jines, Michael P. “Enhancing Genetic Gain in Maize with Tropical Germplasm, QTL Mapping, and Spatial Methodologies.” 2008. Doctoral Dissertation, North Carolina State University. Accessed January 19, 2021. http://www.lib.ncsu.edu/resolver/1840.16/3118.

MLA Handbook (7^th Edition):

Jines, Michael P. “Enhancing Genetic Gain in Maize with Tropical Germplasm, QTL Mapping, and Spatial Methodologies.” 2008. Web. 19 Jan 2021.

Vancouver:

Jines MP. Enhancing Genetic Gain in Maize with Tropical Germplasm, QTL Mapping, and Spatial Methodologies. [Internet] [Doctoral dissertation]. North Carolina State University; 2008. [cited 2021 Jan 19]. Available from: http://www.lib.ncsu.edu/resolver/1840.16/3118.

Council of Science Editors:

Jines MP. Enhancing Genetic Gain in Maize with Tropical Germplasm, QTL Mapping, and Spatial Methodologies. [Doctoral Dissertation]. North Carolina State University; 2008. Available from: http://www.lib.ncsu.edu/resolver/1840.16/3118

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Open Access Theses and Dissertations