subject:( Enzyme Immobilization)

Cornell University

1. Lata, James. Engineering A Rapid Point-Of-Care Diagnostic Platform Utilizing Tethered Enzyme Technology For Time-Sensitive Pathologies.

Degree: PhD, Biomedical Engineering, 2015, Cornell University

URL: http://hdl.handle.net/1813/40952

► The primary advances in creating a point-of-care testing (PoCT) diagnostic platform involve miniaturizing assays based on recognition of biomarker antigens with antibodies. This approach has… (more)

▼ The primary advances in creating a point-of-care testing (PoCT) diagnostic platform involve miniaturizing assays based on recognition of biomarker antigens with antibodies. This approach has inherent issues with time-sensitive pathologies because of the inherently slow speed of antibodyantigen interactions. Such an obstacle can be overcome through use of faster enzymatic reactions. In this work, we focused on creating an enzymatic based PoCT diagnostic platform suitable for timesensitive pathologies, in particular addressing stroke. We hypothesize that by immobilizing enzymes to nanoparticles (NP) the enzymes will be stabilized and confined to a smaller volume, therefore increasing the efficiency of sequential enzymatic reactions needed to detect biomarkers at physiological and pathological levels in human serum and transduce that detection into a luminescent signal. Development of this platform involved several key steps, including immobilizing functional enzymes on inorganic surfaces and developing microfluidic systems with microliter-volume reactions. Because the use of immobilized enzymes in hybrid organic-inorganic devices is in a nascent stage of development, it is also essential to work out fundamental principles of how the inorganic scaffolds might affect the activities of the tethered enzymes. Through biomimicry of the sperm flagellum, enzymes were designed to include a His-tag to bind with Ni-NTA groups on an inorganic surface in a precise orientation. This approach yielded higher specific activity than did attachment of the same enzymes through adsorption or chemically-specific binding (e.g. amine-carboxyl) techniques. While the orientation had dramatic impacts on the enzyme function, the surface size/curvature to which it is bound also influenced the enzyme function. Prior literature on this relationship was largely anecdotal. To identify generalizable truths regarding the iii effects of the surface size and curvature on enzyme function, we used three enzymes, each representing a different class of enzyme, at maximal enzyme loading and in monolayers on gold NPs ranging in size from 5 to 50 nm in diameter. We found that larger NPs had increased enzyme multilayer formations and decreased kcat and specific activity by 2.8 to 8.3 fold, depending on the enzyme. Contrary to other reports and suggesting an advantage for oriented immobilization, we did not see effects of size on the same enzymes when tethered in monolayers. Tethering to NPs has immediate application in medical diagnosis of time-sensitive pathologies due to enzymes' rapid catalysis versus slow antibody interactions. Neuron specific enolase (NSE) is a biomarker for stroke, which we detected through the sequential reaction of tethered luciferase (Luc) and pyruvate kinase (PK), yielding a luminescent signal proportional to the concentration of enolase in our samples. In both rat stroke models and human patient samples, we found this diagnostic platform gave accurate results in 10 minutes compared to typical 4 hour ELISA tests, yet maintained a high… Advisors/Committee Members: Travis,Alexander J. (chair), Erickson,David (committee member), Jin,Moonsoo (committee member).

Subjects/Keywords: Protein Engineering; Diagnostic; Enzyme Immobilization

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APA (6^th Edition):

Lata, J. (2015). Engineering A Rapid Point-Of-Care Diagnostic Platform Utilizing Tethered Enzyme Technology For Time-Sensitive Pathologies. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/40952

Chicago Manual of Style (16^th Edition):

Lata, James. “Engineering A Rapid Point-Of-Care Diagnostic Platform Utilizing Tethered Enzyme Technology For Time-Sensitive Pathologies.” 2015. Doctoral Dissertation, Cornell University. Accessed January 16, 2021. http://hdl.handle.net/1813/40952.

MLA Handbook (7^th Edition):

Lata, James. “Engineering A Rapid Point-Of-Care Diagnostic Platform Utilizing Tethered Enzyme Technology For Time-Sensitive Pathologies.” 2015. Web. 16 Jan 2021.

Vancouver:

Lata J. Engineering A Rapid Point-Of-Care Diagnostic Platform Utilizing Tethered Enzyme Technology For Time-Sensitive Pathologies. [Internet] [Doctoral dissertation]. Cornell University; 2015. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/1813/40952.

Council of Science Editors:

Lata J. Engineering A Rapid Point-Of-Care Diagnostic Platform Utilizing Tethered Enzyme Technology For Time-Sensitive Pathologies. [Doctoral Dissertation]. Cornell University; 2015. Available from: http://hdl.handle.net/1813/40952

Penn State University

2. Gaddes, David Edwin. Remote Calorimetric Biosensing For Clinical Diagnostic Applications.

Degree: 2016, Penn State University

URL: https://submit-etda.libraries.psu.edu/catalog/12544deg5098

► The medical community increasingly relies on quantification of biomarkers in blood, urine, and saliva to monitor treatments and screen for diseases. While glucose meters have… (more)

▼ The medical community increasingly relies on quantification of biomarkers in blood, urine, and saliva to monitor treatments and screen for diseases. While glucose meters have been around since the 1980s only two biosensors have gained success in commercial applications, the glucose meter and the pregnancy test. The majority of biomarkers are quantified using a benchtop analytical system developed specifically for one biomarker. We developed a portable, calorimetric biosensor, which has the capability of quantifying a large variety of biomarkers. Specifically, our goal was to develop this biosensor to quantify creatinine in human urine samples. Further, we sought to develop a sensitive and robust calorimeter, in which the enzyme containing cartridge could be quickly removed and replaced. To achieve this, we implemented a highly sensitive Y-cut quartz crystal resonator as the sensing mechanism in this calorimetric biosensor. The high-temperature sensitivity of the quartz resonator enables a sensitive calorimetric system despite the separation between the fluid of interest and the sensor through 100 μm gap of air. Moreover, to further improve the calorimetric performance of this device we implemented a second quartz resonator, which acts as a reference sensor to eliminate spurious noise from thermal fluctuations. Additionally, we evaluate three enzyme immobilization techniques: glutaraldehyde crosslinking, electrostatic layer-by-layer immobilization, and alginate entrapment using both a fluorescent assay and the calorimetric biosensor. This work is concluded by quantifying the concentration of creatinine in human urine samples. The creatinine concentrations determined using the biosensors were compared against those determined through an HPLC technique. This Ph.D. work has successfully demonstrated the development of a calorimetric biosensor, specifically for the quantification of creatinine in human urine samples. Advisors/Committee Members: Srinivas Tadgiadapa, Dissertation Advisor/Co-Advisor.

Subjects/Keywords: Biosensor; calorimetry; microfabrication; enzyme immobilization

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APA (6^th Edition):

Gaddes, D. E. (2016). Remote Calorimetric Biosensing For Clinical Diagnostic Applications. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/12544deg5098

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gaddes, David Edwin. “Remote Calorimetric Biosensing For Clinical Diagnostic Applications.” 2016. Thesis, Penn State University. Accessed January 16, 2021. https://submit-etda.libraries.psu.edu/catalog/12544deg5098.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gaddes, David Edwin. “Remote Calorimetric Biosensing For Clinical Diagnostic Applications.” 2016. Web. 16 Jan 2021.

Vancouver:

Gaddes DE. Remote Calorimetric Biosensing For Clinical Diagnostic Applications. [Internet] [Thesis]. Penn State University; 2016. [cited 2021 Jan 16]. Available from: https://submit-etda.libraries.psu.edu/catalog/12544deg5098.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gaddes DE. Remote Calorimetric Biosensing For Clinical Diagnostic Applications. [Thesis]. Penn State University; 2016. Available from: https://submit-etda.libraries.psu.edu/catalog/12544deg5098

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Louisiana State University

3. Calixte, Nyote J. From Micro- to Nano-Scale: Applications of Solid-Phase Enzymatic Reactors for Biopolymer Disassembly.

Degree: PhD, Chemistry, 2014, Louisiana State University

URL: etd-08272014-211241 ; https://digitalcommons.lsu.edu/gradschool_dissertations/924

► The process of immobilizing enzymes onto solid supports for bioreactions has some compelling advantages compared to their solution-based counterpart including the facile separation of… (more)

Subjects/Keywords: kinetics; microscale; nanoscale; immobilization; enzyme

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APA (6^th Edition):

Calixte, N. J. (2014). From Micro- to Nano-Scale: Applications of Solid-Phase Enzymatic Reactors for Biopolymer Disassembly. (Doctoral Dissertation). Louisiana State University. Retrieved from etd-08272014-211241 ; https://digitalcommons.lsu.edu/gradschool_dissertations/924

Chicago Manual of Style (16^th Edition):

Calixte, Nyote J. “From Micro- to Nano-Scale: Applications of Solid-Phase Enzymatic Reactors for Biopolymer Disassembly.” 2014. Doctoral Dissertation, Louisiana State University. Accessed January 16, 2021. etd-08272014-211241 ; https://digitalcommons.lsu.edu/gradschool_dissertations/924.

MLA Handbook (7^th Edition):

Calixte, Nyote J. “From Micro- to Nano-Scale: Applications of Solid-Phase Enzymatic Reactors for Biopolymer Disassembly.” 2014. Web. 16 Jan 2021.

Vancouver:

Calixte NJ. From Micro- to Nano-Scale: Applications of Solid-Phase Enzymatic Reactors for Biopolymer Disassembly. [Internet] [Doctoral dissertation]. Louisiana State University; 2014. [cited 2021 Jan 16]. Available from: etd-08272014-211241 ; https://digitalcommons.lsu.edu/gradschool_dissertations/924.

Council of Science Editors:

Calixte NJ. From Micro- to Nano-Scale: Applications of Solid-Phase Enzymatic Reactors for Biopolymer Disassembly. [Doctoral Dissertation]. Louisiana State University; 2014. Available from: etd-08272014-211241 ; https://digitalcommons.lsu.edu/gradschool_dissertations/924

4. Reni, George. Catalysis by Enzymes immobilized on Tuned Mesoporous Silica.

Degree: Applied Chemistry, 2013, Cochin University of Science and Technology

URL: http://dyuthi.cusat.ac.in/purl/4627

►

Mesoporous silica nanoparticles provide a non-invasive and biocompatible delivery platform for a broad range of applications in therapeutics, pharmaceuticals and diagnosis. Additionally, mesoporous silica materials… (more)

Subjects/Keywords: Enzymes as biocatalyst; Immobilization; ideal enzyme supports; Enzyme immobilization; activity of enzymes; Enzyme adsorption isotherms

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APA (6^th Edition):

Reni, G. (2013). Catalysis by Enzymes immobilized on Tuned Mesoporous Silica. (Thesis). Cochin University of Science and Technology. Retrieved from http://dyuthi.cusat.ac.in/purl/4627

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Reni, George. “Catalysis by Enzymes immobilized on Tuned Mesoporous Silica.” 2013. Thesis, Cochin University of Science and Technology. Accessed January 16, 2021. http://dyuthi.cusat.ac.in/purl/4627.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Reni, George. “Catalysis by Enzymes immobilized on Tuned Mesoporous Silica.” 2013. Web. 16 Jan 2021.

Vancouver:

Reni G. Catalysis by Enzymes immobilized on Tuned Mesoporous Silica. [Internet] [Thesis]. Cochin University of Science and Technology; 2013. [cited 2021 Jan 16]. Available from: http://dyuthi.cusat.ac.in/purl/4627.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Reni G. Catalysis by Enzymes immobilized on Tuned Mesoporous Silica. [Thesis]. Cochin University of Science and Technology; 2013. Available from: http://dyuthi.cusat.ac.in/purl/4627

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

5. Ogorzalek, Tadeusz L. Examining the Behavior of Surface Tethered Enzymes.

Degree: PhD, Chemical Biology, 2015, University of Michigan

URL: http://hdl.handle.net/2027.42/111541

► Surface-immobilized enzymes are important for a wide range of technological applications, including industrial catalysis, drug delivery, medical diagnosis and biosensors. However, our understanding of how… (more)

▼ Surface-immobilized enzymes are important for a wide range of technological applications, including industrial catalysis, drug delivery, medical diagnosis and biosensors. However, our understanding of how enzymes and proteins interact with abiological surfaces on the molecular level remains extremely limited. We have compared the structure, activity and thermal stability of beta-galactosidase variants attached to a chemically well-defined self assembled monolayer (SAM) surface. Maleimide-terminated ethylene glycol linkers were used to attach beta-galactosidase through a unique cysteinyl residue. These maleimide-terminated linkers were mixed with ethylene glycol linkers terminated with different chemical moieties to engineer surfaces with varying hydrophobicity and electrostatic charge. In collaboration with the Chen Lab, we used SFG and ATR-FTIR to experimentally measure the orientation of the surface tethered enzyme. In collaboration with the Brooks Lab, we conducted coarse grain model simulations to examine the atomic level interactions between the protein and the surface. Through coarse grain modelling, it was shown that the increased range of motion allowed to an enzyme tethered to a flexible loop region increased the number of protein surface interactions relative to the interactions experienced by an enzyme attached to the helix. For mildly hydrophibic surfaces, such as a full maleimide-terminated SAM, these increased interactions are destabilizing. This was confirmed experimentally by the reduction in thermal stability for beta-galactosidase attached by the loop region. Using SFG, it was shown that the distribution of orientations of an enzyme attached to a loop is greater than to an enzyme tethered to a helix. By varying the electrostatic properties of the terminal groups used in the SAMs, it was shown that beta-galactosidase immobilized onto SAMs containing a mixture of positively and negatively terminated residues retained a higher level of specific activity than surfaces terminated with either uncharged hydrophilic or hydrophobic surfaces. Thermal stability was highest on uncharged hydrophilic surfaces. Overall, we were able to develop a molecular level model for the behavior of surface attached enzymes, and a potential approach for predicting approaches for engineering tethered enzyme systems. Advisors/Committee Members: Marsh, E Neil G. (committee member), Chen, Zhan (committee member), Walter, Nils G. (committee member), O'Brien, Patrick (committee member).

Subjects/Keywords: Enzyme Immobilization; Surface Attachment; Biological Chemistry; Science

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APA (6^th Edition):

Ogorzalek, T. L. (2015). Examining the Behavior of Surface Tethered Enzymes. (Doctoral Dissertation). University of Michigan. Retrieved from http://hdl.handle.net/2027.42/111541

Chicago Manual of Style (16^th Edition):

Ogorzalek, Tadeusz L. “Examining the Behavior of Surface Tethered Enzymes.” 2015. Doctoral Dissertation, University of Michigan. Accessed January 16, 2021. http://hdl.handle.net/2027.42/111541.

MLA Handbook (7^th Edition):

Ogorzalek, Tadeusz L. “Examining the Behavior of Surface Tethered Enzymes.” 2015. Web. 16 Jan 2021.

Vancouver:

Ogorzalek TL. Examining the Behavior of Surface Tethered Enzymes. [Internet] [Doctoral dissertation]. University of Michigan; 2015. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/2027.42/111541.

Council of Science Editors:

Ogorzalek TL. Examining the Behavior of Surface Tethered Enzymes. [Doctoral Dissertation]. University of Michigan; 2015. Available from: http://hdl.handle.net/2027.42/111541

Cornell University

6. Andler, Stephanie. Immobilized Enzymes for Waste Stream Valorization.

Degree: PhD, Food Science and Technology, 2018, Cornell University

URL: http://hdl.handle.net/1813/59598

► Food industry waste streams represent a significant environmental burden. These waste streams, rich in sugars, proteins, and lipids, can be transformed into value-added products. Enzymes… (more)

▼ Food industry waste streams represent a significant environmental burden. These waste streams, rich in sugars, proteins, and lipids, can be transformed into value-added products. Enzymes provide a selective and greener route of synthesis, but lack stability in extreme environments, and the ability to be reused. Therefore, the present work studied immobilized enzymes for practical application in two major waste streams: oil and dairy. First, lipase B from Candida antarctica (E.C. 3.1.1.3) was immobilized within cross-linked microparticles (CLMP) displaying varying scales of support, and the influence of these nano- and macroscale support materials on lipase activity and stability was studied. Kinetic analysis and extreme environment testing revealed that that polydicyclopentadiene macrostructure had the greatest influence on stability. These CLMPs successfully produced surfactants from lauric acid and D-glucose. However, the ~10 µm particles were unrecoverable from viscous solvents. Next, lipase was immobilized into macroscale, polymerized high internal phase emulsions (HIPE) to facilitate ease of handling. After fabrication, HIPEs displayed an increase in activity (139±9.7%) over native lipase. However, lipase was weakly immobilized, and retained less than 10% relative activity after 5 washes. HIPEs retained above 40% relative activity after exposure to pH 3, 7, and 10 for 24 hours, effectively expanding the working range of lipase. HIPEs also displayed an increase in relative activity after storage at 20 ˚C and 40 ˚C for 24 hours and retained greater than 40% activity after storage in 50 ˚C and 60 ˚C for 24 hours. These macroscale HIPEs were recoverable from reaction media but require further optimization to increase protein retention. Finally, β-galactosidase from Aspergillus oryzae (E.C. 3.2.1.23) and glucose isomerase (E.C. 5.3.1.5) were co-immobilized within a cross-linked enzyme aggregate (combi-CLEA) for dairy waste upcycling. The β-galactosidase within the combi-CLEA retained approximately 10% activity after immobilization, but activity was unable to be detected from glucose isomerase. Additional optimization of the combi-CLEA is necessary before application testing in whey permeate. Applications-based approaches to enzyme immobilization are necessary to create low-cost, industrially relevant catalysts. Through the understanding of support material influences, and extreme environment testing, enzymes have the potential to increase the overall sustainability of food processing. Advisors/Committee Members: Goddard, Julie Melissa (chair), Sacks, Gavin Lavi (committee member), Nugen, Sam Rasmussen (committee member).

Subjects/Keywords: bioprocessing; enzyme; immobilization; waste stream; Food science

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APA (6^th Edition):

Andler, S. (2018). Immobilized Enzymes for Waste Stream Valorization. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/59598

Chicago Manual of Style (16^th Edition):

Andler, Stephanie. “Immobilized Enzymes for Waste Stream Valorization.” 2018. Doctoral Dissertation, Cornell University. Accessed January 16, 2021. http://hdl.handle.net/1813/59598.

MLA Handbook (7^th Edition):

Andler, Stephanie. “Immobilized Enzymes for Waste Stream Valorization.” 2018. Web. 16 Jan 2021.

Vancouver:

Andler S. Immobilized Enzymes for Waste Stream Valorization. [Internet] [Doctoral dissertation]. Cornell University; 2018. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/1813/59598.

Council of Science Editors:

Andler S. Immobilized Enzymes for Waste Stream Valorization. [Doctoral Dissertation]. Cornell University; 2018. Available from: http://hdl.handle.net/1813/59598

University of New South Wales

7. Hou, Jingwei. Titania Based Bio-catalytic Membrane Systems for Bisphenol-A Degradation and CO2 Capture.

Degree: Chemical Sciences & Engineering, 2014, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/54572 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:35312/SOURCE02?view=true

► Over the last decades, the sharp increases in industrial polycarbonate and epoxy production and fossil fuel consumption have resulted in emerging environmental challenges like micropollutant… (more)

▼ Over the last decades, the sharp increases in industrial polycarbonate and epoxy production and fossil fuel consumption have resulted in emerging environmental challenges like micropollutant and greenhouse gas, which require novel mitigation approaches. Enzyme catalyzed reactions have been recognized as promising approaches for many environmental applications. Among different enzymes, laccase and carbonic anhydrase have attracted wide spread interest in wastewater treatment and CO2 capture respectively. However, the application of free enzyme is restricted due to the difficulty in recycle and reuse. Therefore, efficient enzyme immobilization and bio-reactor design are required.Based on a series of comprehensive experimental researches, the performance of TiO2 nanoparticle and TiO2 nanoparticle functionalized membrane as laccase immobilization support has been evaluated. Different immobilization techniques were compared and the sequential process was regarded as the optimal option due to its relatively high activity and stability. It was also found that the nanoparticle size, surface charge, and laccase accessibility also had significant effect on the immobilized laccase performance.TiO2 functionalized membranes were prepared with both nanoparticle blending and sol-gel coating processes, then laccase was immobilized on the resultant membranes. Compared with the blended membrane, the coated membrane provided more accessible TiO2 for laccase immobilization thus resulted in better performances. The effect of coating layer surface architectural properties and the membrane pore size on the laccase immobilization performance was also discussed.In this study, two laccase based membrane bioreactors were proposed for bisphenol-A (BPA) degradation: the bio-catalytic membrane reactor and the bio-catalytic nanoparticle suspension membrane hybrid reactor. For both reactors, satisfactory BPA removal was achieved through enzymatically degradation and membrane filtration process. In addition, the performance of the hybrid reactor under simulated wastewater harsh conditions was assessed to evaluate the feasibility of using such reactors for municipal water treatment.Finally, the feasibility of applying the TiO2 nanoparticle and TiO2 coated polymer membrane as carbonic anhydrase immobilization support was also explored. This study also prepared a novel TiO2 functionalized hydrophilic-hydrophobic membrane for carbonic anhydrase immobilization. Such a bio-catalytic membrane was further used for CO2 hydration with a gas-liquid contactor, and nearly 100 % improvement in CO2 hydration rate was obtained Advisors/Committee Members: CHEN, VICKI, Chemical Sciences & Engineering, Faculty of Engineering, UNSW.

Subjects/Keywords: CO2 hydration; Enzyme immobilization; Micropollutant removal

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APA (6^th Edition):

Hou, J. (2014). Titania Based Bio-catalytic Membrane Systems for Bisphenol-A Degradation and CO2 Capture. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/54572 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:35312/SOURCE02?view=true

Chicago Manual of Style (16^th Edition):

Hou, Jingwei. “Titania Based Bio-catalytic Membrane Systems for Bisphenol-A Degradation and CO2 Capture.” 2014. Doctoral Dissertation, University of New South Wales. Accessed January 16, 2021. http://handle.unsw.edu.au/1959.4/54572 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:35312/SOURCE02?view=true.

MLA Handbook (7^th Edition):

Hou, Jingwei. “Titania Based Bio-catalytic Membrane Systems for Bisphenol-A Degradation and CO2 Capture.” 2014. Web. 16 Jan 2021.

Vancouver:

Hou J. Titania Based Bio-catalytic Membrane Systems for Bisphenol-A Degradation and CO2 Capture. [Internet] [Doctoral dissertation]. University of New South Wales; 2014. [cited 2021 Jan 16]. Available from: http://handle.unsw.edu.au/1959.4/54572 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:35312/SOURCE02?view=true.

Council of Science Editors:

Hou J. Titania Based Bio-catalytic Membrane Systems for Bisphenol-A Degradation and CO2 Capture. [Doctoral Dissertation]. University of New South Wales; 2014. Available from: http://handle.unsw.edu.au/1959.4/54572 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:35312/SOURCE02?view=true

University of Akron

8. Chen, Xi. Infrared and Uv-Vis Spectroscopic Studies of Catalytic Reaction of Enzymes and Immobilization Enzyme on Porous Polymers.

Degree: MS, Polymer Science, 2015, University of Akron

URL: http://rave.ohiolink.edu/etdc/view?acc_num=akron1428327122

► Biomimetic catalysis has great impact on the development of the research of organic catalysis1. Immobilized enzyme used as catalysts has attracted a lot of attention… (more)

Subjects/Keywords: Polymers; Enzyme, immobilization, UV-vis, infrared

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APA (6^th Edition):

Chen, X. (2015). Infrared and Uv-Vis Spectroscopic Studies of Catalytic Reaction of Enzymes and Immobilization Enzyme on Porous Polymers. (Masters Thesis). University of Akron. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=akron1428327122

Chicago Manual of Style (16^th Edition):

Chen, Xi. “Infrared and Uv-Vis Spectroscopic Studies of Catalytic Reaction of Enzymes and Immobilization Enzyme on Porous Polymers.” 2015. Masters Thesis, University of Akron. Accessed January 16, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=akron1428327122.

MLA Handbook (7^th Edition):

Chen, Xi. “Infrared and Uv-Vis Spectroscopic Studies of Catalytic Reaction of Enzymes and Immobilization Enzyme on Porous Polymers.” 2015. Web. 16 Jan 2021.

Vancouver:

Chen X. Infrared and Uv-Vis Spectroscopic Studies of Catalytic Reaction of Enzymes and Immobilization Enzyme on Porous Polymers. [Internet] [Masters thesis]. University of Akron; 2015. [cited 2021 Jan 16]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron1428327122.

Council of Science Editors:

Chen X. Infrared and Uv-Vis Spectroscopic Studies of Catalytic Reaction of Enzymes and Immobilization Enzyme on Porous Polymers. [Masters Thesis]. University of Akron; 2015. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron1428327122

9. Navya, Antony. Immobilization of Diastase α-amylase on to synthetic and natural polymers.

Degree: Applied Chemistry, 2014, Cochin University of Science and Technology

URL: http://dyuthi.cusat.ac.in/purl/4696

►

Several natural and synthetic supports have been assessed for their efficiency for enzyme immobilization. Synthetic polymer materials are prepared by chemical polymerization using various monomers.… (more)

Subjects/Keywords: Biocatalysis; Enzymes; Nature’s catalysts; Enzyme immobilization; Methods of enzyme immobilization; Synthetic polymer-polypyrrole

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APA (6^th Edition):

Navya, A. (2014). Immobilization of Diastase α-amylase on to synthetic and natural polymers. (Thesis). Cochin University of Science and Technology. Retrieved from http://dyuthi.cusat.ac.in/purl/4696

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Navya, Antony. “Immobilization of Diastase α-amylase on to synthetic and natural polymers.” 2014. Thesis, Cochin University of Science and Technology. Accessed January 16, 2021. http://dyuthi.cusat.ac.in/purl/4696.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Navya, Antony. “Immobilization of Diastase α-amylase on to synthetic and natural polymers.” 2014. Web. 16 Jan 2021.

Vancouver:

Navya A. Immobilization of Diastase α-amylase on to synthetic and natural polymers. [Internet] [Thesis]. Cochin University of Science and Technology; 2014. [cited 2021 Jan 16]. Available from: http://dyuthi.cusat.ac.in/purl/4696.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Navya A. Immobilization of Diastase α-amylase on to synthetic and natural polymers. [Thesis]. Cochin University of Science and Technology; 2014. Available from: http://dyuthi.cusat.ac.in/purl/4696

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Sydney

10. Tran, Clara. Plasma activated polymers for yeast and enzyme immobilization .

Degree: 2013, University of Sydney

URL: http://hdl.handle.net/2123/10127

► This thesis investigates plasma immersion ion implantation (PIII) for immobilizing enzymes and yeast cells with the aim of developing new processes for cellulosic ethanol production.… (more)

Subjects/Keywords: yeast immobilization; enzyme immobilization; plasma immersion ion implantation; cellulosic ethanol; surface charge

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APA (6^th Edition):

Tran, C. (2013). Plasma activated polymers for yeast and enzyme immobilization . (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/10127

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tran, Clara. “Plasma activated polymers for yeast and enzyme immobilization .” 2013. Thesis, University of Sydney. Accessed January 16, 2021. http://hdl.handle.net/2123/10127.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tran, Clara. “Plasma activated polymers for yeast and enzyme immobilization .” 2013. Web. 16 Jan 2021.

Vancouver:

Tran C. Plasma activated polymers for yeast and enzyme immobilization . [Internet] [Thesis]. University of Sydney; 2013. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/2123/10127.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tran C. Plasma activated polymers for yeast and enzyme immobilization . [Thesis]. University of Sydney; 2013. Available from: http://hdl.handle.net/2123/10127

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade do Rio Grande do Sul

11. Volpato, Giandra. Produção, purificação e imobilização de lipases de Staphylococcus warneri EX17 produzidas em glicerol.

Degree: 2009, Universidade do Rio Grande do Sul

URL: http://hdl.handle.net/10183/15543

► Lipases (EC 3.1.1.3) são um grupo de enzimas que catalisam a hidrólise e síntese de triacilgliceróis. Estas enzimas apresentam estabilidade em diversos solventes orgânicos, podendo… (more)

▼ Lipases (EC 3.1.1.3) são um grupo de enzimas que catalisam a hidrólise e síntese de triacilgliceróis. Estas enzimas apresentam estabilidade em diversos solventes orgânicos, podendo ser aplicadas como biocatalisadores em vários processos anteriormente realizados apenas por catalisadores químicos. Este trabalho teve como objetivo produzir, purificar e imobilizar lipases de Staphylococcus warneri EX17, cepa capaz de utilizar glicerol como fonte de carbono. Inicialmente, as condições de cultivo para produção de lipases foram otimizadas através de duas ferramentas de planejamento experimental: delineamento Placket Burman (P-B) e delineamento composto central rotacional (DCCR). Determinou-se que as melhores condições para produção desta enzima são: temperatura de 36 °C; pH 8,1; 30 g/L de glicerol; 3,0 g/L de óleo de oliva e 2,5 g/L de óleo de soja. Também se verificou ser possível a utilização de glicerol residual, oriundo da síntese enzimática de biodiesel como fonte de carbono. O extrato enzimático mostrou-se estável em três solventes orgânicos testados (metanol, etanol e η-hexano). Ainda visando a otimização das condições de cultivo, foram realizados cultivos submersos em biorreatores a fim de estudar a influência da taxa volumétrica de transferência de oxigênio (kLa) e do controle do pH, na produção da enzima. A maior produção de lipases ocorreu quando aplicado um kLa de 38 h-1 e com o pH controlado em 7,0 ao longo do cultivo, o que permitiu aumentar a produção da enzima em 5 vezes, em relação ao obtido nas condições anteriormente empregadas. A purificação da lipase foi realizada baseando-se no mecanismo de ativação interfacial destas enzimas sobre superfícies hidrofóbicas. Duas resinas foram testadas, octil-Sepharose e butil- Toyopearl. A lipase produzida foi purificada em apenas um passo utilizando esta última resina. Foi estudada a hiperativação da lipase purificada na presença de detergentes, a atividade lipolítica foi aumentada em 2,5 vezes na presença de 0,1% de Triton X-100. A lipase purificada foi imobilizada através de três estratégias: adsorção em suporte hidrofóbico; união covalente unipontual e união covalente multipontual. A influência da imobilização na modulação das propriedades da enzima foi estudada. A lipase apresentou maior estabilidade quando imobilizada multipontualmente. A hidrólise de distintos ésteres quirais pelos diferentes biocatalisadores obtidos também foi estudada. Os ésteres utilizados foram: (±) mandelato de metila, (±)-2-O-butiril-2-fenilacético e (±)-2-hidroxi-4-fenilbutirato de etilo. A especificidade da enzima foi muito dependente do método de imobilização, sendo que a lipase imobilizada unipontualmente foi mais específica para o substrato (±)-2-hidroxi-4-fenilbutirato de etilo, enquanto que para os outros dois substratos foi a lipase adsorvida hidrofobicamente. Este estudo demonstrou que a lipase de S. warneri EX17 pode ser produzida utilizando glicerol residual como fonte de carbono, levando a diminuição do custo na produção da enzima, que apresenta propriedades bastante… Advisors/Committee Members: Ayub, Marco Antônio Záchia.

Subjects/Keywords: Lipase; Staphylococcus warneri; Lipase production; Glicerol; Catalisadores; Enzyme purification; Enzyme immobilization; Biotecnologia

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Volpato, G. (2009). Produção, purificação e imobilização de lipases de Staphylococcus warneri EX17 produzidas em glicerol. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/15543

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Volpato, Giandra. “Produção, purificação e imobilização de lipases de Staphylococcus warneri EX17 produzidas em glicerol.” 2009. Thesis, Universidade do Rio Grande do Sul. Accessed January 16, 2021. http://hdl.handle.net/10183/15543.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Volpato, Giandra. “Produção, purificação e imobilização de lipases de Staphylococcus warneri EX17 produzidas em glicerol.” 2009. Web. 16 Jan 2021.

Vancouver:

Volpato G. Produção, purificação e imobilização de lipases de Staphylococcus warneri EX17 produzidas em glicerol. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2009. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/10183/15543.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Volpato G. Produção, purificação e imobilização de lipases de Staphylococcus warneri EX17 produzidas em glicerol. [Thesis]. Universidade do Rio Grande do Sul; 2009. Available from: http://hdl.handle.net/10183/15543

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Univerzitet u Beogradu

12. Žuža, Milena G., 1980-. Razvoj imobilisanih sistema sa penicilin-acilazom iz Esherichia coli za dobijanje polusintetskih penicilina.

Degree: Tehnološko-metalurški fakultet, 2013, Univerzitet u Beogradu

URL: https://fedorabg.bg.ac.rs/fedora/get/o:5526/bdef:Content/get

►

Hemija i hemijska tehnologija - Biohemijsko inženjerstvo i biotehnologija / Chemistry and Chemical Technology - Biochemical Engineering and Biotechnology

Osnovni cilj ove doktorske disertacije je… (more)

▼

Hemija i hemijska tehnologija - Biohemijsko inženjerstvo i biotehnologija / Chemistry and Chemical Technology - Biochemical Engineering and Biotechnology

Osnovni cilj ove doktorske disertacije je bio optimizacija postupka dobijanja 6- aminopenicilanske kiseline primenom imobilisane penicilin-acilaze iz E. coli. Pri tome je razmatrana reakcija hidrolize prirodnog penicilina G do 6-APA katalizovane slobodnom i imobilisanom penicilin-acilazom. Da bi se realizovao postavljeni cilj bilo je potrebno izvršiti izbor nosača i metode za imobilizaciju enzima, optimizovati postupak imobilizacije enzima sa aspekta mase vezanog enzima i prinosa aktivnosti, okarakterisati dobijeni biokatalizator i ispitati razlike u kinetičkim parametrima slobodne i imobilisane penicilin-acilaze, izabrati odgovarajuće reaktorsko rešenje za izvođenje reakcije sa imobilisanim enzimima i ispitati operativnu stabilnost sistema. U prvom delu rada je izvršena karakterizacija slobodne penicilin-acilaze iz Escherichia coli i ispitana su njena katalitička svojstva u reakciji hidrolize penicilina G kao referentnom sistemu. Ova karakterizacija je bila neophodna da bi se utvrdile razlike u delovanju slobodnog i imobilisanog enzima. U tom cilju utvrđen je sadržaj proteina u komercijalnom enzimskom preparatu, specifična aktivnost, pH i temperaturni profil, termalna stabilnost, kao i vrednosti kinetičkih konstanti, i to Mihaelisove konstante i maksimalne brzine reakcije. Isto tako, ispitan je uticaj inhibicije supstratom u višku i proizvodima reakcije na brzinu reakcije u sistemu sa slobodnim enzimom i u tom cilju je određena vrsta inhibicije i vrednosti konstanti inhibicije. U drugom delu rada osnovni cilj istraživanja je bio usmeren na stabilizaciju enzima različitim postupcima. Pri tome je ispitano nekoliko postupaka hemijske imobilizacije enzima na različitim prirodnim i sintetskim polimerima (Sepabeads sa različitim funkcionalnim grupama i hitozan), kao i postupak imobilizacije prethodno hemijski modifikovanog enzima. U radu je ispitana mogućnost direktnog vezivanja penicilin-acilaze preko amino grupa u molekulu za epoksidne grupe nosača, zatim vezivanje enzima za nosač koji je prethodno aktiviran glutaraldehidom ili vezivanje prethodno modifikovanog enzima za nosače sa amino grupama...

Advisors/Committee Members: Knežević-Jugović, Zorica, 1969-.

Subjects/Keywords: penicillin acylase; immobilization; Sepabeads carriers; enzyme modification; chitosan microbeads; enzyme biocatalysis; kinetics; bioreactors

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Žuža, Milena G., 1. (2013). Razvoj imobilisanih sistema sa penicilin-acilazom iz Esherichia coli za dobijanje polusintetskih penicilina. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:5526/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Žuža, Milena G., 1980-. “Razvoj imobilisanih sistema sa penicilin-acilazom iz Esherichia coli za dobijanje polusintetskih penicilina.” 2013. Thesis, Univerzitet u Beogradu. Accessed January 16, 2021. https://fedorabg.bg.ac.rs/fedora/get/o:5526/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Žuža, Milena G., 1980-. “Razvoj imobilisanih sistema sa penicilin-acilazom iz Esherichia coli za dobijanje polusintetskih penicilina.” 2013. Web. 16 Jan 2021.

Vancouver:

Žuža, Milena G. 1. Razvoj imobilisanih sistema sa penicilin-acilazom iz Esherichia coli za dobijanje polusintetskih penicilina. [Internet] [Thesis]. Univerzitet u Beogradu; 2013. [cited 2021 Jan 16]. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:5526/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Žuža, Milena G. 1. Razvoj imobilisanih sistema sa penicilin-acilazom iz Esherichia coli za dobijanje polusintetskih penicilina. [Thesis]. Univerzitet u Beogradu; 2013. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:5526/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

13. Jia, Feng. Design of novel nano-carriers for multi-enzyme co-localization.

Degree: 2013, Iowa State University

URL: https://lib.dr.iastate.edu/etd/13378

► The widely existing MECs in Nature have inspired researchers to design synthetic analogs to promote the overall catalytic efficiency in vitro by co-localizing multiple enzymes… (more)

▼ The widely existing MECs in Nature have inspired researchers to design synthetic analogs to promote the overall catalytic efficiency in vitro by co-localizing multiple enzymes to mimic the MECs' unique functionalities. A number of efforts have been devoted to designing synthetic MECs in the past couples of decades. This thesis work has focused on developing novel strategies based on enzyme immobilization to design nano-carriers for multi-enzyme co-localization to realize kinetics enhancement and strong control of spatial arrangement of the enzymes. Three distinct approaches have been designed using different attachment methods and platforms. First, the multifunctional polystyrene nanoparticles were designed for immobilization and sequential co-localization of GOX and SHRP enzymes using covalent binding and streptavidin-biotin coupling attachment techniques. The sequentially co-localized GOX and SHRP on B/C-PS nanoparticles were capable of enhancing the overall conversion rate by approximately two-fold compared to the equivalent concentration of free enzymes in solution. Secondly, amphiphilic Pluronic-QD micelles were designed to co-localize multiple enzymes to investigate the effect of a more flexible substrate compared to the rigid polystyrene particles. FRET was used to characterize the adsorption of single enzymes and co-localization of multiple enzymes on the micelles. The two co-localized enzymes enhanced the overall conversion rate by ~100% compared to the equivalent concentration of free enzymes in solution, which is very comparable to the findings in the first study. To further investigate the spatial arrangement impact on enzyme co-localization, the precise DNA hybridization was investigated to direct multi-enzyme co-localization on PS nanoparticles. It was found that the co-localized GOX and HRP via DNA hybridization significantly improved the overall reaction efficiency by close to 200% as compared to single enzyme immobilization mixture. The co-localized enzymes also exhibited well stability over time. In summary, the current research has demonstrated the superior potential of co-localized multiple enzymes in terms of kinetically-driven benefits. The spatial arrangement plays a significant role in mimicking the MECs in vitro. Looking forward, the design of sustainable and re-usable multi-enzyme biocatalysts would lead to both scientifically exciting research as well as economically viable designs for next generation catalysts and biosensors.

Subjects/Keywords: Enzyme immobilization; Multi-enzyme co-localization; Nanoparticles; Nanotechnology; Polymers; Biochemistry; Chemical Engineering; Nanoscience and Nanotechnology

11 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jia, F. (2013). Design of novel nano-carriers for multi-enzyme co-localization. (Thesis). Iowa State University. Retrieved from https://lib.dr.iastate.edu/etd/13378

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Jia, Feng. “Design of novel nano-carriers for multi-enzyme co-localization.” 2013. Thesis, Iowa State University. Accessed January 16, 2021. https://lib.dr.iastate.edu/etd/13378.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Jia, Feng. “Design of novel nano-carriers for multi-enzyme co-localization.” 2013. Web. 16 Jan 2021.

Vancouver:

Jia F. Design of novel nano-carriers for multi-enzyme co-localization. [Internet] [Thesis]. Iowa State University; 2013. [cited 2021 Jan 16]. Available from: https://lib.dr.iastate.edu/etd/13378.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jia F. Design of novel nano-carriers for multi-enzyme co-localization. [Thesis]. Iowa State University; 2013. Available from: https://lib.dr.iastate.edu/etd/13378

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

KTH

14. Öh, Clara. Biosensor based on immobilized amine transaminase for detection of amphetamine.

Degree: Biotechnology and Health (CBH), 2020, KTH

URL: http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-278584

►

Amine transaminases (ATA) catalyse the transfer of an amino group from one molecule and replaces a ketone or aldehyde with the amino group, the… (more)

▼

Amine transaminases (ATA) catalyse the transfer of an amino group from one molecule and replaces a ketone or aldehyde with the amino group, the amino group on the amino-donor is replaced with a ketone or aldehyde. This enzyme, ATA from Chromobacterium violaceum, has previously been used to catalyse the reaction involving amphetamine, therefore, it might be possible to use this enzyme to convert amphetamine and the product absorbs in the UV spectrum and can therefore be measured spectrophotometrically. The aim of the project was to explore the possibility of using ATA in a portable biosensor for the detection of amphetamine. A literature study of commercially available portable biosensors was performed, activity of the free enzyme was tested against two substrates, methylbenzylamine (MBA) and amphetamine. Research on immobilization techniques, materials, and surface functionalization was done to chose suitable methods for immobilizing ATA. Two immobilization methods were suggested and one of the methods, ionic immobilization through His-tag towards Ni2+ on the surface, was tested for enzyme activity toward MBA. The enzyme activity of the free enzyme in solution towards MBA was comparable to previously reported enzyme activity, however, no enzyme activity towards amphetamine was observed. No activity was observed for the immobilized enzyme, but it might be due to the experimental design, more experiments need to be performed to draw conclusions.

Amintransaminaser (ATA) katalyserar överförandet av en amingrupp från en molekyl och ersätter en keton eller aldehyd med den amingruppen, amingruppen på amin-donatorn ersätts med en keton eller aldehyd. Det här enzymet, ATA från Chromobacterium violaceum (CvATA), har tidigare använts för att katalysera en reaktion som involverar amfetamin, därför skulle detta enzym kunna användas på amfetamin. Produkten av reaktionen absorberar i UV spektrumet och kan mätas med en spektrofotometer. Målet med projektet var att utforska möjligheten av att använda CvATA i en biosensor för att detektera amfetamin. En litteraturstudie på kommersiellt tillgängliga bärbara biosensorer genomfördes, aktiviteten av det fria enzymet testades mot två substrat, metylbenzylamin (MBA) och amfetamin. Information samlades om immobiliseringstekniker, material, och ytfunktionalisering gjordes för att välja ut lämpliga metoder för immobilisering av CvATA. Två immobiliseringsmetoder föreslogs och en av metoderna, immobilisering via enzymets His6-tagg och Ni2+ joner på ytan, testades för enzymaktivitet mot MBA. Enzymaktiviteten av det fria enzymet i lösning mot MBA var i samma storleksordning som tidigare rapporterad enzymaktivitet, men ingen enzymaktivitet mot amfetamin kunde observeras. Ingen aktivitet kunde observeras för det immobiliserade enzymet, men det kan vara på grund av designen på experimentet, fler experiment behöver göras för att kunna dra några fler slutsatser.

Subjects/Keywords: amine transaminases (ATA); enzyme immobilization; biosensor; covalent immobilization; ionic immobilization; amphetamine; histidine-tagged enzyme; Poly(methyl methacrylate) (PMMA); Medical Biotechnology; Medicinsk bioteknologi

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Öh, C. (2020). Biosensor based on immobilized amine transaminase for detection of amphetamine. (Thesis). KTH. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-278584

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Öh, Clara. “Biosensor based on immobilized amine transaminase for detection of amphetamine.” 2020. Thesis, KTH. Accessed January 16, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-278584.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Öh, Clara. “Biosensor based on immobilized amine transaminase for detection of amphetamine.” 2020. Web. 16 Jan 2021.

Vancouver:

Öh C. Biosensor based on immobilized amine transaminase for detection of amphetamine. [Internet] [Thesis]. KTH; 2020. [cited 2021 Jan 16]. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-278584.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Öh C. Biosensor based on immobilized amine transaminase for detection of amphetamine. [Thesis]. KTH; 2020. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-278584

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

15. Crespilho, Frank Nelson. Membranas eletroativas nanoestruturadas: estudo de transporte de carga e imobilização enzimática.

Degree: PhD, Físico-Química, 2007, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/75/75131/tde-25102007-111702/ ;

►

Esta tese aborda quatro tópicos fundamentais para o desenvolvimento e aplicação de membranas eletroativas nanoestruturadas (MENs): (i) síntese e caracterização de nanopartículas (Nps) de prata,… (more)

▼

Esta tese aborda quatro tópicos fundamentais para o desenvolvimento e aplicação de membranas eletroativas nanoestruturadas (MENs): (i) síntese e caracterização de nanopartículas (Nps) de prata, ouro e platina encapsuladas em moléculas de dendrímero poliamidoamina geração 4 (PAMAM); (ii) preparação de filmes automontados contendo PAMAM e Nps de ouro (PAMAM-Au); (iii) preparação de MENs utilizando sistema core-shell [email protected], onde Me é um mediador redox; (iv) imobilização enzimática em MENs e estudos biocatalíticos associados a processos eletroquímicos. As Nps foram caracterizadas observando-se a banda plasmônica em espectros na região do UV-Vis. Imagens de microscopia eletrônica de transmissão revelaram que PAMAM-Au e PAMAM-Pt possuem morfologias esféricas, enquanto o PAMAM-Ag forma grandes cristais com estruturas fractais. Estruturas cúbicas de face centrada caracterizaram os cristais formados de Au e Pt, sendo possível estimar os diâmetros (3,0 nm) das Nps pela equação de Scherrer em difratogramas de raios X, confirmados posteriormente por microscopia eletrônica por transmissão (TEM). Um indício de estabilização por encapsulamento do híbrido PAMAM-Au foi obtido de espectros de infravermelho (FTIR), a partir de modificações nas bandas das amidas. A cinética de reação para formação de PAMAM-Au também foi estudada. Filmes de PVS/PAMAM-Au (onde PVS é o poli(ácidovinilssulfônico)) foram preparados com 5 minutos de imersão, com a mesma quantidade de material sendo adsorvida em cada camada, segundo medidas de espectroscopia UV-Vis e voltametria cíclica (CV). No caso do eletrodo ITO-(PVS/PAMAM-Au), saltos de elétrons foram considerados o mecanismo de transporte de carga ao longo do filme. Um novo sistema core-shell [email protected] foi preparado, formando um sistema ITO-(PVS/PAMAM-Au)[email protected], em que a eletrodeposição de PB (azul da Prússia) foi monitorada medindo-se as correntes faradaicas durante os ciclos potenciodinâmicos. Outros mediadores de hexacianoferratos de metais de transição (Fe, Ni, Co e Cu) foram obtidos sobre eletrodos de ITO-(PVS/PAMAM-Au). De resultados de espectroscopia de impedância eletroquímica (EIS), verificou-se que a resistência de transporte de carga decresce na sequência CuHCF > FeHCF > NiHCF > CoHCF e todos os eletrodos apresentaram atividade catalítica para o peróxido de hidrogênio. Uma nova configuração de eletrodo, ITO-(PVS/PAMAM-Au)[email protected], pôde ser aplicada como dispositivo enzimático, com a glicose oxidase (GOx) sendo imobilizada por drop-coating na superfície do eletrodo e aplicada em experimentos de biocatálise. A glicose pôde ser detectada a 0,0 V (Ag/AgCl), com resposta linear até 100 µmol L-1 de glicose, sensibilidade de 115 nA mmol L-1, limite de detecção de 5,5 µmol L-1 e KMapp de 0,24 mmol L-1, mostrando que o sistema aqui proposto cria um ambiente propício para a enzima operar com alta atividade catalítica.

This thesis addresses four fundamental topics for producing and applying electroactive nanostructured membranes (ENMs): (i) synthesis of Au, Pt and Ag nanoparticles (Nps) using…

Advisors/Committee Members: Nart, Francisco Carlos.

Subjects/Keywords: electroactive nanostructured membranes; enzyme immobilization; imobilização enzimática; membranas eletroativas nanoestruturadas

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Crespilho, F. N. (2007). Membranas eletroativas nanoestruturadas: estudo de transporte de carga e imobilização enzimática. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/75/75131/tde-25102007-111702/ ;

Chicago Manual of Style (16^th Edition):

Crespilho, Frank Nelson. “Membranas eletroativas nanoestruturadas: estudo de transporte de carga e imobilização enzimática.” 2007. Doctoral Dissertation, University of São Paulo. Accessed January 16, 2021. http://www.teses.usp.br/teses/disponiveis/75/75131/tde-25102007-111702/ ;.

MLA Handbook (7^th Edition):

Crespilho, Frank Nelson. “Membranas eletroativas nanoestruturadas: estudo de transporte de carga e imobilização enzimática.” 2007. Web. 16 Jan 2021.

Vancouver:

Crespilho FN. Membranas eletroativas nanoestruturadas: estudo de transporte de carga e imobilização enzimática. [Internet] [Doctoral dissertation]. University of São Paulo; 2007. [cited 2021 Jan 16]. Available from: http://www.teses.usp.br/teses/disponiveis/75/75131/tde-25102007-111702/ ;.

Council of Science Editors:

Crespilho FN. Membranas eletroativas nanoestruturadas: estudo de transporte de carga e imobilização enzimática. [Doctoral Dissertation]. University of São Paulo; 2007. Available from: http://www.teses.usp.br/teses/disponiveis/75/75131/tde-25102007-111702/ ;

Texas A&M University

16. Mahadevan, Aishwarya. Comparison of Molecular-Wires for Enhancing Charge Transport of Enzymatic Electrode Assemblies: A Glycerol Bioanode Model.

Degree: MS, Biological and Agricultural Engineering, 2014, Texas A&M University

URL: http://hdl.handle.net/1969.1/154062

► Biomolecules are inherently less conductive. Therefore, bio-electronic devices that depend on conventional biomolecules to tether enzymes onto electrode supports and to shuttle electrons between the… (more)

Subjects/Keywords: Bioanode; Enzyme immobilization; Glycerol dehydrogenase; Molecular wiring; Self-assembled monolayers

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mahadevan, A. (2014). Comparison of Molecular-Wires for Enhancing Charge Transport of Enzymatic Electrode Assemblies: A Glycerol Bioanode Model. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/154062

Chicago Manual of Style (16^th Edition):

Mahadevan, Aishwarya. “Comparison of Molecular-Wires for Enhancing Charge Transport of Enzymatic Electrode Assemblies: A Glycerol Bioanode Model.” 2014. Masters Thesis, Texas A&M University. Accessed January 16, 2021. http://hdl.handle.net/1969.1/154062.

MLA Handbook (7^th Edition):

Mahadevan, Aishwarya. “Comparison of Molecular-Wires for Enhancing Charge Transport of Enzymatic Electrode Assemblies: A Glycerol Bioanode Model.” 2014. Web. 16 Jan 2021.

Vancouver:

Mahadevan A. Comparison of Molecular-Wires for Enhancing Charge Transport of Enzymatic Electrode Assemblies: A Glycerol Bioanode Model. [Internet] [Masters thesis]. Texas A&M University; 2014. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/1969.1/154062.

Council of Science Editors:

Mahadevan A. Comparison of Molecular-Wires for Enhancing Charge Transport of Enzymatic Electrode Assemblies: A Glycerol Bioanode Model. [Masters Thesis]. Texas A&M University; 2014. Available from: http://hdl.handle.net/1969.1/154062

University of California – Berkeley

17. Palla, Kanwal. Development and Applications of N-terminal Protein Bioconjugation Reactions.

Degree: Chemistry, 2016, University of California – Berkeley

URL: http://www.escholarship.org/uc/item/5mv8940t

► With highly evolved structures and function, proteins have an extraordinarily diverse range of capabilities. In order to take advantage of their unparalleled specificity in the… (more)

Subjects/Keywords: Chemistry; Bioconjugation; Chemical Biology; Enzyme immobilization; Protein Chemistry; Protein Modification

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Palla, K. (2016). Development and Applications of N-terminal Protein Bioconjugation Reactions. (Thesis). University of California – Berkeley. Retrieved from http://www.escholarship.org/uc/item/5mv8940t

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Palla, Kanwal. “Development and Applications of N-terminal Protein Bioconjugation Reactions.” 2016. Thesis, University of California – Berkeley. Accessed January 16, 2021. http://www.escholarship.org/uc/item/5mv8940t.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Palla, Kanwal. “Development and Applications of N-terminal Protein Bioconjugation Reactions.” 2016. Web. 16 Jan 2021.

Vancouver:

Palla K. Development and Applications of N-terminal Protein Bioconjugation Reactions. [Internet] [Thesis]. University of California – Berkeley; 2016. [cited 2021 Jan 16]. Available from: http://www.escholarship.org/uc/item/5mv8940t.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Palla K. Development and Applications of N-terminal Protein Bioconjugation Reactions. [Thesis]. University of California – Berkeley; 2016. Available from: http://www.escholarship.org/uc/item/5mv8940t

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Univerzitet u Beogradu

18. Luković, Nevena. Развој ензимског поступка за синтезу метил естара масних киселина.

Degree: Tehnološko-metalurški fakultet, 2016, Univerzitet u Beogradu

URL: https://fedorabg.bg.ac.rs/fedora/get/o:11830/bdef:Content/get

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Технолошко инжењерство - Engineering technology

У оквиру ове тезе испитивана је могућност ензимског поступка синтезе метил естара масних киселина (биодизела) из биљног уља, поступком трансестерификације.… (more)

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Технолошко инжењерство - Engineering technology

У оквиру ове тезе испитивана је могућност ензимског поступка синтезе метил естара масних киселина (биодизела) из биљног уља, поступком трансестерификације. Испитано је каталитичко дејство липаза из различитих извора са аспекта трансестерификационе активности и стабилности у системима без органског растварача. Користиле су се комерцијалне липазе из различитих микроорганизама (Candida rugosa, Rhizomucor miehei, Candida antarctica) а потом је методом вишефакторних експерименталних планова (централни композициони ротатабилни план) испитан утицај различитих параметара: температуре, садржаја воде у систему, количине додатог ензима, моларног односа метанол/уље као и начина извођења процеса на принос метил естара масних киселина. Испитана је могућност примене различитих ацил акцептора и утврђено је да се као најбољи показао метил ацетат. Захваљујући примени овог ацил акцептора омогућена је оптимизација реакторског система. Испитали су се утицаји различитих процесних параметара и режима рада биореактора на продуктивност имобилисаног система. Други део рада се фокусирао на технике имобилизације. Анализирана је ковалентна имобилизација нативне липазе из C. rugosa и нативне липазе из C. antarctica на полимерним носачима које садрже епоксидне групе и афинитет добијеног имобилисаног ензима према супстрату. Испитана је ефикасност модификације комерцијалног носача Eupergit® C 250L помоћу цистеина и глутаралдехида ради добијања имолиблисаног система веће стабилности. Утврђено је да се овако имобилисана липаза из C. antarctica може успешно користити као биокатализатор у синтези биодизела, при чему се применом ове липазе добијају приноси биодизела у рангу приноса које дају комерцијални препарати са имобилисаном липазом из C. antarctica.

Advisors/Committee Members: Knežević-Jugović, Zorica, 1969-.

Subjects/Keywords: fatty acid methyl esters; biodiesel; lipases; transesterification; enzyme immobilization

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Luković, N. (2016). Развој ензимског поступка за синтезу метил естара масних киселина. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:11830/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Luković, Nevena. “Развој ензимског поступка за синтезу метил естара масних киселина.” 2016. Thesis, Univerzitet u Beogradu. Accessed January 16, 2021. https://fedorabg.bg.ac.rs/fedora/get/o:11830/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Luković, Nevena. “Развој ензимског поступка за синтезу метил естара масних киселина.” 2016. Web. 16 Jan 2021.

Vancouver:

Luković N. Развој ензимског поступка за синтезу метил естара масних киселина. [Internet] [Thesis]. Univerzitet u Beogradu; 2016. [cited 2021 Jan 16]. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:11830/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Luković N. Развој ензимског поступка за синтезу метил естара масних киселина. [Thesis]. Univerzitet u Beogradu; 2016. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:11830/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Univerzitet u Beogradu

19. Prokopijević, Miloš M., 1982-. Imobilizacija peroksidaze iz soje (Glycine max) na makroporoznom glicidil-metakrilatu i hemijski modifikovanom pektinu.

Degree: Hemijski fakultet, 2018, Univerzitet u Beogradu

URL: https://fedorabg.bg.ac.rs/fedora/get/o:17096/bdef:Content/get

►

Biohemija - Enzimologija, imobilizacija enzima / Biochemistry - Enzymology, enzyme immobilization

Peroksidaza iz soje (SBP, E.C. 1.11.1.7) je enzim koji pripada klasi III biljnih peroksidaza… (more)

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Biohemija - Enzimologija, imobilizacija enzima / Biochemistry - Enzymology, enzyme immobilization

Peroksidaza iz soje (SBP, E.C. 1.11.1.7) je enzim koji pripada klasi III biljnih peroksidaza i katalizuje oksidaciju i polimerizaciju različitih fenolnih jedinjenja u prisustvu vodonik peroksida kao supstrata. Nalazi se u semenom omotaču soje koji predstavlja jeftini nusproizvod i polazni materijal za izolovanje velike količine sirovog enzima. Izolovana SBP je imobilizovana primenom dve kovalentne metode na seriju makroporoznih kopolimera poli(GMA-co-EGDMA) različitih dijametara pora. Glutaraldehidni metod imobilizacije se pokazao boljim od perjodatnog a najveća specifična aktivnost od 23 IU/g dobijena je na kopolimeru veličine pora od 120 nm. Imobilizovana peroksidaza pokazala je veću termalnu stabilnost i stabilnost u organskom rastvaraču, aktivnost u širem opsegu pH i 2,86 puta veću Km vrednost za pirogalol u odnosu na rastvorni enzim. Sintetisana je serija modifikovanih pektina oksidacijom perjodatom u molarnom odnosu od 2,5 do 20 mol% i naknadnom reduktivnom aminacijom tiraminom i cijanoborhidridom. Dobijeni tiramin-pektini prave stabilne hidrogelove umrežavanjem fenolnih grupa u reakciji katalizovanoj peroksidazama u prisustvu vodonik peroksida. SBP je uspešno imobilizovana unutar mikro-kuglica hidrogela nastalih enzimskom polimerizacijom modifikovanog pektina u emulziji sa najvećom specifičnom aktivnošću postignutom na tiramin-pektinu oksidovanom 5 mol% perjodatom. Imobilizovana SBP je pored šireg pH optimuma pokazala i veću termalnu stabilnost i stabilnost u organskom rastvaraču u odnosu na slobodan enzim. Mikrokuglice sa imobilizovanom SBP zadržale su 50% početne aktivnosti nakon 7 ciklusa ponovne upotrebe za oksidaciju pirogalola u bač reaktoru. Pod optimalnim uslovima sa unutrašnjom dostavom vodonik peroksida postignuto je uklanjanje fenola od 64% imobilizovanom SBP na poli(GMA-co-EGDMA) veličine pora od 120 nm.

Advisors/Committee Members: Prodanović, Radivoje, 1972-.

Subjects/Keywords: Soybean peroxidase; enzyme immobilization; glycidyl-methacrylate; periodate; glutaraldehyde; hydrogel; pectin

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Prokopijević, Miloš M., 1. (2018). Imobilizacija peroksidaze iz soje (Glycine max) na makroporoznom glicidil-metakrilatu i hemijski modifikovanom pektinu. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:17096/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Prokopijević, Miloš M., 1982-. “Imobilizacija peroksidaze iz soje (Glycine max) na makroporoznom glicidil-metakrilatu i hemijski modifikovanom pektinu.” 2018. Thesis, Univerzitet u Beogradu. Accessed January 16, 2021. https://fedorabg.bg.ac.rs/fedora/get/o:17096/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Prokopijević, Miloš M., 1982-. “Imobilizacija peroksidaze iz soje (Glycine max) na makroporoznom glicidil-metakrilatu i hemijski modifikovanom pektinu.” 2018. Web. 16 Jan 2021.

Vancouver:

Prokopijević, Miloš M. 1. Imobilizacija peroksidaze iz soje (Glycine max) na makroporoznom glicidil-metakrilatu i hemijski modifikovanom pektinu. [Internet] [Thesis]. Univerzitet u Beogradu; 2018. [cited 2021 Jan 16]. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:17096/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Prokopijević, Miloš M. 1. Imobilizacija peroksidaze iz soje (Glycine max) na makroporoznom glicidil-metakrilatu i hemijski modifikovanom pektinu. [Thesis]. Univerzitet u Beogradu; 2018. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:17096/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université Catholique de Louvain

20. van den Biggelaar, , Ludivine. Design of heterogeneous biocatalysts for flow chemical processes : towards a greener transamination reaction.

Degree: 2018, Université Catholique de Louvain

URL: http://hdl.handle.net/2078.1/207945

►

For decades, the drug manufacture has been using polluting reagents that are released in the environment. Nowadays, greener production modes are being targeted. In this… (more)

▼

For decades, the drug manufacture has been using polluting reagents that are released in the environment. Nowadays, greener production modes are being targeted. In this direction, biocatalysis probably represents a decisive opportunity. Transaminases (TA) have already been used for the green production of chiral amines (drug precursors). There are however two main limitations to the use of TA in such organic synthesis: (1) the difficulty to recover the free enzymes after a batch reaction, (2) the low yield for chiral amines, owing to unfavourable thermodynamics. To tackle these challenges, the main objectives of this thesis are (i) developing heterogeneous biocatalysts using suitable carriers and efficient immobilization methods; (ii) developing a continuous flow biocatalytic reactor for transamination reactions; (iii) implementing strategies for the displacement of the thermodynamic equilibrium. TA are efficiently immobilized onto APTES-functionalized macrocellular silica monoliths through covalent grafting using glutaraldehyde as a coupling agent. The monoliths can be mounted in a simple flow set-up. In the flow mode, TA are active and stable over long period of time, and enantioselectivity is retained. An optimized APTES functionalization method was developed, that allowed to boost the enzymatic activity. The influence of temperature on enzyme impregnation was also highlighted. Hydrophobic silica monoliths were studied as supports, but, hydrophobicity appeared to have no impact on the enzyme immobilization and activity. Using a three-enzyme system to consume the co-product, the equilibrium could be displaced towards the formation of the chiral amine. In flow mode, such strategy was successful only when the added enzymes were free and in high concentration. Finally, we show that our flow system can be used to immobilize another type of enzyme (tyrosine ammonia-lyase), to design other efficient flow mode biocatalytic processes for amine synthesis.

(AGRO - Sciences agronomiques et ingénierie biologique) – UCL, 2018

Advisors/Committee Members: UCL - SST/IMCN/MOST - Molecules, Solids and Reactivity, UCL - Ingénierie biologique, agronomique et environnementale, Debecker, Damien, Soumillion, Patrice, Garcia, Yann, Gaigneaux, Eric, Luis Alconero, Patricia, Paradisi, Francesca, Boissière, Cédric.

Subjects/Keywords: Enzyme immobilization; Flow chemistry; Transaminase; Biocatalysis; Silanization; Silica monolith

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

van den Biggelaar, , L. (2018). Design of heterogeneous biocatalysts for flow chemical processes : towards a greener transamination reaction. (Thesis). Université Catholique de Louvain. Retrieved from http://hdl.handle.net/2078.1/207945

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

van den Biggelaar, , Ludivine. “Design of heterogeneous biocatalysts for flow chemical processes : towards a greener transamination reaction.” 2018. Thesis, Université Catholique de Louvain. Accessed January 16, 2021. http://hdl.handle.net/2078.1/207945.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

van den Biggelaar, , Ludivine. “Design of heterogeneous biocatalysts for flow chemical processes : towards a greener transamination reaction.” 2018. Web. 16 Jan 2021.

Vancouver:

van den Biggelaar, L. Design of heterogeneous biocatalysts for flow chemical processes : towards a greener transamination reaction. [Internet] [Thesis]. Université Catholique de Louvain; 2018. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/2078.1/207945.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

van den Biggelaar, L. Design of heterogeneous biocatalysts for flow chemical processes : towards a greener transamination reaction. [Thesis]. Université Catholique de Louvain; 2018. Available from: http://hdl.handle.net/2078.1/207945

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Toronto

21. Mastour Tehrani, Sepehr. Novel Aqueous Microgels and Their Applications.

Degree: PhD, 2017, University of Toronto

URL: http://hdl.handle.net/1807/79332

► This thesis describes the design, synthesis, and some new applications of aqueous microgels. One research objective was to prepare lanthanide-containing microgels which are resistant to… (more)

▼ This thesis describes the design, synthesis, and some new applications of aqueous microgels. One research objective was to prepare lanthanide-containing microgels which are resistant to lanthanide ion leaching in aqueous media and can be used in bead-based assays. I report the synthesis of lanthanide phosphate (LnPO4) nanocrystals embedded in poly(NIPAM/VCL/MAA) copolymer microgels. Here the microgels were first ion-exchanged with Ln3+ and then treated with PBS buffer to form LnPO4 crystals. The TEM images of the TbPO4-containing microgels in combination with SAXS studies show remarkable needle-like nanocrystals distributed throughout the microgel. Mass cytometry studies showed that these microgels resist ion leaching in different buffers. Another direction in my Ph.D. research was to design and synthesize temperature-invariant aqueous microgels that would preserve their dimensions and colloidal stability upon heating in water, and can serve as a host for enzymes in high-temperature reactions. For this purpose, I prepared poly(N-hydroxyethyl acrylamide) (PHEAA) microgels through a two-step synthesis. First, I prepared a precursor particle of poly[di(ethylene glycol) acrylate] in water, and then, these particles were subjected to aminolysis in dioxane with a mixture of ethanolamine and ethylenediamine to form to PHEAA-NH2 microgels. As a proof-of-concept study, I attached the enzyme horseradish peroxidase (HRP) to the microgels. Kinetic studies showed that immobilization led to improved enzyme stability at high temperatures compared to the native enzyme. I also examined the effect of the thermal response of microgels on the activity of enzymes. I prepared the thermo-responsive poly[oligo(ethylene glycol) methacrylate] (PEGMA) microgels and attached aminotetraethyleneglycol pendant groups to these microgels to act as a spacer and to make them biofunctionalizable. I found no discontinuous change in the activity of the immobilized enzyme as the microgel was heated through its VPTT. This study serves a guide for the biofunctionalization of protein repellent PEGMA microgels. Advisors/Committee Members: Winnik, Mitchelle A., Chemical Engineering Applied Chemistry.

Subjects/Keywords: enzyme; immobilization; lanthanide; microgel; nanocrystals; precipitation polymerization; 0542

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mastour Tehrani, S. (2017). Novel Aqueous Microgels and Their Applications. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/79332

Chicago Manual of Style (16^th Edition):

Mastour Tehrani, Sepehr. “Novel Aqueous Microgels and Their Applications.” 2017. Doctoral Dissertation, University of Toronto. Accessed January 16, 2021. http://hdl.handle.net/1807/79332.

MLA Handbook (7^th Edition):

Mastour Tehrani, Sepehr. “Novel Aqueous Microgels and Their Applications.” 2017. Web. 16 Jan 2021.

Vancouver:

Mastour Tehrani S. Novel Aqueous Microgels and Their Applications. [Internet] [Doctoral dissertation]. University of Toronto; 2017. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/1807/79332.

Council of Science Editors:

Mastour Tehrani S. Novel Aqueous Microgels and Their Applications. [Doctoral Dissertation]. University of Toronto; 2017. Available from: http://hdl.handle.net/1807/79332

Georgia State University

22. Jiang, Wenxin. The Preliminary Study on the Role of 1-Hexene Monooxygenase in Delayed Fruit Ripening by Rhodococcus rhodochrous DAP 96253.

Degree: PhD, Biology, 2016, Georgia State University

URL: https://scholarworks.gsu.edu/biology_diss/171

► Rhodococcus rhodochrous DAP 96253, a well-known industrial bacterium, had various 1-hexene monooxygenase (1-HMO) activities when grown on YEMEA plates supplemented with eight different carbohydrates.… (more)

▼ Rhodococcus rhodochrous DAP 96253, a well-known industrial bacterium, had various 1-hexene monooxygenase (1-HMO) activities when grown on YEMEA plates supplemented with eight different carbohydrates. Besides, 1-HMO exhibited different storage temperature preferences. Lactose could induce the highest 1-HMO activity in R. rhodochrous DAP 96253 while the cells showed the lowest 1-HMO activity when trehalose was the supplement. The 1-HMO activity of R. rhodochrous DAP 96253 was not maintained when stored at 37°C as well as at 4°C and 25°C. Trehalose-induced 1-HMO activity of R. rhodochrous DAP 96253 was more stable from Day 0 to Day 21 at all these three temperatures, compared with the other seven carbohydrates. Immobilization of enzymes can maintain enzyme activity longer, offer easier enzyme storage conditions and make some enzymes reusable, much research has been done in this area. In this study, R. rhodochrous DAP 96253, grown on YEMEA plates supplemented by glucose and urea, was investigated using whole bananas as the inducer of 1-HMO activity and different immobilization methods to maintain this enzyme activity. It was shown that calcium-alginate polyvinyl alcohol (PVA) beads could maintain 1-HMO activity of R. rhodochrous DAP 96253 more stable than calcium-alginate beads. Whole bananas exhibited very obvious effects of inducing 1-HMO activity of R. rhodochrous DAP 96253. A number of recent studies have clearly demonstrated that induced cells of R. rhodochrous DAP 96253 can prolong the shelf-life of post-harvested fruits. With USDA estimates of 40% of all harvested produce in the US not being consumed because of loss of quality, the ability to extend the period of ripeness of produce has great potential to improve the quality of nutrition. Modification or degradation of those signals (primary and secondary) associated with ripening in fruit or the perception of those signals represents a potential mode of action for delayed ripening by induced cells of R. rhodochrous DAP 96253. Ethylene and cyanide are the two primary signals in ripening. In this study, the role of 1-HMO from induced cells was investigated by time-course experiments focusing on 1-HMO activity and stability. In addition, fruit volatile organic compounds (VOCs) were detected and compared by GC-FID and GC/MS over the course of fruit ripening. The results show a correlation between 1-HMO activity and stability in delayed fruit ripening. It was further demonstrated that the presence of secondary signal fruit VOCs enhanced 1-HMO activity. Aromatic profiles of treated fruits, by GC-FID and GC/MS, show a consistent picture of VOCs associated with earlier fruit ripening stages. Advisors/Committee Members: Dr. George E. Pierce, Dr. Sidney A. Crow, Dr. Eric S. Gilbert.

Subjects/Keywords: Rhodococcus; 1-HMO; Carbohydrates; Enzyme Immobilization; Delayed Fruit Ripening

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jiang, W. (2016). The Preliminary Study on the Role of 1-Hexene Monooxygenase in Delayed Fruit Ripening by Rhodococcus rhodochrous DAP 96253. (Doctoral Dissertation). Georgia State University. Retrieved from https://scholarworks.gsu.edu/biology_diss/171

Chicago Manual of Style (16^th Edition):

Jiang, Wenxin. “The Preliminary Study on the Role of 1-Hexene Monooxygenase in Delayed Fruit Ripening by Rhodococcus rhodochrous DAP 96253.” 2016. Doctoral Dissertation, Georgia State University. Accessed January 16, 2021. https://scholarworks.gsu.edu/biology_diss/171.

MLA Handbook (7^th Edition):

Jiang, Wenxin. “The Preliminary Study on the Role of 1-Hexene Monooxygenase in Delayed Fruit Ripening by Rhodococcus rhodochrous DAP 96253.” 2016. Web. 16 Jan 2021.

Vancouver:

Jiang W. The Preliminary Study on the Role of 1-Hexene Monooxygenase in Delayed Fruit Ripening by Rhodococcus rhodochrous DAP 96253. [Internet] [Doctoral dissertation]. Georgia State University; 2016. [cited 2021 Jan 16]. Available from: https://scholarworks.gsu.edu/biology_diss/171.

Council of Science Editors:

Jiang W. The Preliminary Study on the Role of 1-Hexene Monooxygenase in Delayed Fruit Ripening by Rhodococcus rhodochrous DAP 96253. [Doctoral Dissertation]. Georgia State University; 2016. Available from: https://scholarworks.gsu.edu/biology_diss/171

University of New South Wales

23. Ji, Chao. Laccase-based Biocatalytic Systems for Recalcitrant Micro-Pollutants Degradation and Energy Generation.

Degree: Chemical Engineering, 2017, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/58253 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:45663/SOURCE02?view=true

► The increasing worldwide contamination of freshwater system with micro-pollutants emerges as a critical environmental problem, which has driven the search for novel mitigation approaches. The… (more)

▼ The increasing worldwide contamination of freshwater system with micro-pollutants emerges as a critical environmental problem, which has driven the search for novel mitigation approaches. The use of enzymes such as laccase as biocatalyst has been recognised as a promising approach for micro-pollutants removal. However, rapid denaturation of the free enzyme and its difficulty in recycling and reuse restrict its wider application, and efficient enzyme immobilization and bioreactor design are required.In this study, and two membrane bioreactors were proposed for carbamazepine (CBZ) degradation: the hybrid membrane system where laccase-immobilized TiO2 nanoparticles were suspended in the feed solution, and the biocatalytic membrane reactor where laccase was immobilised on TiO2 coated membrane surface. Using p-coumaric acid as a mediator, efficient CBZ removal (up to 71%) was achieved with the hybrid membrane reactor. Functionalized TiO2 nanoparticles were further applied to immobilize crude enzyme extracts from P. ostreatus culture. The resultant biocatalytic particles had comparable performance to the immobilized purified commercial laccase and showed efficient bisphenol-A and CBZ removal in the hybrid reactor.In addition, a cross-linked carbon nanotubes (CNTs) based membrane was prepared, which exhibited high effectiveness as support for physical adsorption of laccase. The active laccase coating on CNTs membrane can be renewed after simple cleaning and re-immobilization. The biocatalytic membrane also showed substantial improvement in micro-pollutant removal compared with the membrane having no enzyme. At last, it is demonstrated that the intramolecular electron transfer within single enzyme molecule is an important alternative pathway which can be harnessed to generate electricity. By decoupling the redox reaction within laccase, efficient electricity production from unconventional fuels including recalcitrant pollutants and/or toxic organic was obtained in a sole-laccase based enzymatic fuel cell. The intramolecular electron-harnessing concept was also demonstrated with other enzymes, including the power generation during CO2 bioconversion to formate catalysed by formate dehydrogenase. The novel enzymatic power generation is shown to be potentially feasible utilizing wastewater as fuel as well as occurring in tandem with driving bioconversion of chemical feedstock from CO2. Advisors/Committee Members: Chen, Vicki, Chemical Engineering, Faculty of Engineering, UNSW.

Subjects/Keywords: Micro-pollutant; Laccase; Biocatalytic membrane reactor; Enzymatic fuel cell; Enzyme immobilization

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ji, C. (2017). Laccase-based Biocatalytic Systems for Recalcitrant Micro-Pollutants Degradation and Energy Generation. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/58253 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:45663/SOURCE02?view=true

Chicago Manual of Style (16^th Edition):

Ji, Chao. “Laccase-based Biocatalytic Systems for Recalcitrant Micro-Pollutants Degradation and Energy Generation.” 2017. Doctoral Dissertation, University of New South Wales. Accessed January 16, 2021. http://handle.unsw.edu.au/1959.4/58253 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:45663/SOURCE02?view=true.

MLA Handbook (7^th Edition):

Ji, Chao. “Laccase-based Biocatalytic Systems for Recalcitrant Micro-Pollutants Degradation and Energy Generation.” 2017. Web. 16 Jan 2021.

Vancouver:

Ji C. Laccase-based Biocatalytic Systems for Recalcitrant Micro-Pollutants Degradation and Energy Generation. [Internet] [Doctoral dissertation]. University of New South Wales; 2017. [cited 2021 Jan 16]. Available from: http://handle.unsw.edu.au/1959.4/58253 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:45663/SOURCE02?view=true.

Council of Science Editors:

Ji C. Laccase-based Biocatalytic Systems for Recalcitrant Micro-Pollutants Degradation and Energy Generation. [Doctoral Dissertation]. University of New South Wales; 2017. Available from: http://handle.unsw.edu.au/1959.4/58253 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:45663/SOURCE02?view=true

University of Michigan

24. Liu, Yuwei. Surface Studies on the Structure and Functionality of Bioactive Materials.

Degree: PhD, Chemistry, 2014, University of Michigan

URL: http://hdl.handle.net/2027.42/109042

► Bioactive materials are critical in many applications in the fields ranging from antibiofouling coatings to tissue engineering to biosensing devices. The construction of bioactive materials… (more)

▼ Bioactive materials are critical in many applications in the fields ranging from antibiofouling coatings to tissue engineering to biosensing devices. The construction of bioactive materials generally involves dispersion of bioactive components such as biocides, peptides or proteins in organic or inorganic matrices. However, when these bioactive components are incorporated to such matrices, their functionalities may be significantly hindered. As a result, it has spurred great attention seeking guidelines for future rational design and development of bioactive materials with optimal functionalities. This thesis proposes optimization strategies by investigating interfacial molecular interactions of various bioactive materials in situ using surface specific spectroscopies and correlating the structural and orientational information to their activity. Firstly, two biocide-incorporated antifouling polymeric materials are characterized using sum frequency generations vibrational spectroscopy at solid/liquid interfaces. The interaction mechanism is unraveled by varying both biocidal and the polymer components of the system. The structure of the alkyl chains from the biocidal components is determined and by comparing to their antifouling performance, it is concluded that the protruding-out state offers the best antifouling activity. Factors that affect the structure of the biocidal components are examined. Secondly, antimicrobial peptides are immobilized to various surface platforms. The immobilization processes are thoroughly monitored and the final immobilized peptide structures and orientations are determined using spectroscopic technique. The immobilization environment, the location of the attachment point and the peptide sequence all play important roles in determining the immobilization process and the final state of the immobilized peptides. Finally, a model enzyme is immobilized to solid surfaces and characterized by using combined spectroscopic measurements. These results are further correlated to the enzymatic activities and confirmed by molecular dynamics simulations performed by our collaborators. It is concluded that the surface properties, the location of the attachment point, the secondary structure of the attachment point are factors influencing the immobilized enzyme structure and further determining the activity and stability of the immobilized enzyme. In all, this thesis provides systematic understanding how structural differences are related to the difference in the functionality of the bioactive materials under study based on which the optimization designing rules are proposed. Advisors/Committee Members: Chen, Zhan (committee member), Kuroda, Kenichi (committee member), McNeil, Anne Jennifer (committee member), Marsh, E Neil G. (committee member).

Subjects/Keywords: Surface Analysis; Sum Frequency Generation; Antibiofouling Coatings; Antimicrobial Peptide Immobilization; Enzyme Immobilization; Structure-activity Relationship; Chemistry; Science

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Liu, Y. (2014). Surface Studies on the Structure and Functionality of Bioactive Materials. (Doctoral Dissertation). University of Michigan. Retrieved from http://hdl.handle.net/2027.42/109042

Chicago Manual of Style (16^th Edition):

Liu, Yuwei. “Surface Studies on the Structure and Functionality of Bioactive Materials.” 2014. Doctoral Dissertation, University of Michigan. Accessed January 16, 2021. http://hdl.handle.net/2027.42/109042.

MLA Handbook (7^th Edition):

Liu, Yuwei. “Surface Studies on the Structure and Functionality of Bioactive Materials.” 2014. Web. 16 Jan 2021.

Vancouver:

Liu Y. Surface Studies on the Structure and Functionality of Bioactive Materials. [Internet] [Doctoral dissertation]. University of Michigan; 2014. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/2027.42/109042.

Council of Science Editors:

Liu Y. Surface Studies on the Structure and Functionality of Bioactive Materials. [Doctoral Dissertation]. University of Michigan; 2014. Available from: http://hdl.handle.net/2027.42/109042

Univerzitet u Beogradu

25. Prlainović, Nevena Ž. Proučavanje mehanizma enzimske sinteze 4,6-disupstituisanih-3-cijano-2-piridona.

Degree: Tehnološko-metalurški fakultet, 2013, Univerzitet u Beogradu

URL: https://fedorabg.bg.ac.rs/fedora/get/o:5781/bdef:Content/get

►

Hemija i hemijska tehnologija-Organska hemija / Chemistry and Chemical Technology - Organic Chemistry

U okviru ove teze, pomoću lipaze iz Candida rugosa, sintetisani su 4,6-disupstituisani-3-cijano-2-piridoni.… (more)

Subjects/Keywords: lipase from Candida rugosa; 4; 6-disubstituted-3-cyano-2-pyridones; enzyme kinetic; immobilization of enzyme; multiwalled carbon nanotubes; Sephadex

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APA (6^th Edition):

Prlainović, N. �. (2013). Proučavanje mehanizma enzimske sinteze 4,6-disupstituisanih-3-cijano-2-piridona. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:5781/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Prlainović, Nevena Ž. “Proučavanje mehanizma enzimske sinteze 4,6-disupstituisanih-3-cijano-2-piridona.” 2013. Thesis, Univerzitet u Beogradu. Accessed January 16, 2021. https://fedorabg.bg.ac.rs/fedora/get/o:5781/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Prlainović, Nevena Ž. “Proučavanje mehanizma enzimske sinteze 4,6-disupstituisanih-3-cijano-2-piridona.” 2013. Web. 16 Jan 2021.

Vancouver:

Prlainović N�. Proučavanje mehanizma enzimske sinteze 4,6-disupstituisanih-3-cijano-2-piridona. [Internet] [Thesis]. Univerzitet u Beogradu; 2013. [cited 2021 Jan 16]. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:5781/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Prlainović N�. Proučavanje mehanizma enzimske sinteze 4,6-disupstituisanih-3-cijano-2-piridona. [Thesis]. Univerzitet u Beogradu; 2013. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:5781/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade do Rio Grande do Sul

26. Zaluski, Franciele. Imobilização de beta-glicosidase em quitosana e aplicação visando a melhora do perfil aromático de vinhos.

Degree: 2015, Universidade do Rio Grande do Sul

URL: http://hdl.handle.net/10183/130765

►

As β-glicosidades são enzimas que catalisam a hidrólise de ligações glicosídicas. São amplamente encontradas na natureza em plantas, frutas e animais. Possuem diversas aplicações biotecnologicas… (more)

▼

As β-glicosidades são enzimas que catalisam a hidrólise de ligações glicosídicas. São amplamente encontradas na natureza em plantas, frutas e animais. Possuem diversas aplicações biotecnologicas podendo ser amplamente empregadas na indústria de alimentos e bebidas afim de melhorar a qualidade de aroma, sabor, coloração e viscosidade do produto. Este estudo apresenta o processo de imobilização de uma β-glicosidase comercial em suporte de quitosana e a obtenção de um derivado ativo e estável, para ser aplicado no processamento de vinhos aumentando a complexidade aromática de vinhos joven. A imobilizaçãpo foi realizada em suporte de quitosana, reticulado com glutaraldeído, atingindo 100% de eficiência na imobilização com 50mg de proteína por grama de suporte e 65% de atividade recuperada no derivado imobilizado. A imobilização além de contribuir para um maior controle do processo, alterou algumas características da β-glicosidase, a qual demonstrou manter uma atividade mais alta em faixas mais amplas de pH, quando comparada a enzima livre. A β-glicosidase imobilizada apresentou grande estabilidade podendo ser reutilizada por mais de 30 ciclos, mantendo sua atividade inicial. A aplicação da β-glicosidase no vinho foi realizada em batelada, por um tempo de 90 min, sob agitação. A análise por SPME/GC-MS revelou um aumento na concentração terpenos, quando comparada a amostras não tratadas. Houve um aumento na concentração de geraniol, citronelol, linalol e nerol. A aplicação da β-glicosidase foi bem sucedida, liberando os compostos aromáticos em um curto períuodo de tempo de contato. O processo de reutilização mostra que o biocatalisador imobilizado é uam ferramenta vantajosa para a indústria de bebidas.

β-glucosidases are enzymes that catalyze the hydrolysis of glycosidic bonds. They are widely found in nature at plants, fruits and animals. They have various biotechnological applications being largely used in food and beverage industry for the enhance the product viscosity, coloration, flavour and aroma qualities. This study presents a commercial β-glucosidase immobilization in chitosan support in order to obtain an active and stable derivative, enabling its application in winemaking, enhancing the aromatic complexity in young wines. The immobilization process was conducted in chitosana support, cross-linked with glutaraldehyde, reaching 100% efficiency in immobilization with 50 mg of protein per gram of support and 65% recovered activity in imobilized derived. The immobilization of the enzyme contributes to greater control of the process, changed some features of β-glucosidase, which proved to be more stable at pH changes when compared to free enzyme. Also the immobilized β-glucosidase showed great operational stability been reused for more than 30 cycles maintaining its initial activity. The application of β-glucosidase in the wine was held in batch for 90 minutes under stirring. The analyzis by SPME / GC-MS revelead a increasement in terpens concentration when compared to the sample without treatment. Was noticed a…

Advisors/Committee Members: Hertz, Plinho Francisco.

Subjects/Keywords: β-glucosidase; Beta-glicosidase; Enzyme immobilization; Aromatização de alimento; Vinho; Chitosan; Enzima; Wine; Aroma

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zaluski, F. (2015). Imobilização de beta-glicosidase em quitosana e aplicação visando a melhora do perfil aromático de vinhos. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/130765

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zaluski, Franciele. “Imobilização de beta-glicosidase em quitosana e aplicação visando a melhora do perfil aromático de vinhos.” 2015. Thesis, Universidade do Rio Grande do Sul. Accessed January 16, 2021. http://hdl.handle.net/10183/130765.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zaluski, Franciele. “Imobilização de beta-glicosidase em quitosana e aplicação visando a melhora do perfil aromático de vinhos.” 2015. Web. 16 Jan 2021.

Vancouver:

Zaluski F. Imobilização de beta-glicosidase em quitosana e aplicação visando a melhora do perfil aromático de vinhos. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2015. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/10183/130765.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zaluski F. Imobilização de beta-glicosidase em quitosana e aplicação visando a melhora do perfil aromático de vinhos. [Thesis]. Universidade do Rio Grande do Sul; 2015. Available from: http://hdl.handle.net/10183/130765

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Alberta

27. Nish, Gordon L. Optimization of the reaction conditions of two enzymes for use in a carbon sequestration process, and investigation into immobilization via encapsulation within polymersomes.

Degree: MS, Department of Chemical and Materials Engineering, 2016, University of Alberta

URL: https://era.library.ualberta.ca/files/cv405s966t

► Carbon dioxide emissions from human activities contribute to an increase of greenhouse gases in the atmosphere. In nature, this gas is sequestered through the use… (more)

▼ Carbon dioxide emissions from human activities contribute to an increase of greenhouse gases in the atmosphere. In nature, this gas is sequestered through the use of enzymes found in the Calvin-Benson-Bassham cycle, with useful molecules such as sugars being synthesized as products. A biomimetic approach to capturing carbon dioxide and using it to synthesize useful chemicals, by way of these enzymes in a bioprocess, has been proposed. To achieve this, active enzymes must be harvested and their kinetic properties need to be characterized. Optimal process conditions must be established, with an appropriate enzyme immobilization technique being applied to ameliorate the overall bioprocess. In this work, the phosphoribulokinase enzyme was produced in the bacterial cell platform Escherichia coli using molecular cloning techniques. The kinetic conditions affecting the rates of the enzymes ribose 5-phosphate isomerase A from E. coli, and phosphoribulokinase from Synechococcus elongatus, were locally optimized through surface response methodology and mathematical modeling. These models predicted the optimal pH levels, temperature, substrate concentration and other factors used in the assays. The rate of ribose 5-phosphate isomerase product formation under optimized conditions showed an increase of about 37 % over the measured rate under initial conditions, while that of phosphoribulokinase activity increased around 21 %. Enzyme characterization revealed Km constants for the sugar substrates to be 0.12 mM for phosphoribulokinase and 9.4 mM for ribose 5-phosphate isomerase, with half-lives of 177 minutes and 89 hours, respectively, at room temperature. Furthermore, immobilization via encapsulation within polymersomes was investigated by using a digestive assay and fluorescence correlation spectroscopy. The encapsulation efficiency of the enzyme Rpi was found to be about 23 %. This represents a final enzyme concentration of 1.2 µM having been encapsulated. The enzyme phosphoribulokinase was successfully purified from a bacterial platform. This enzyme and ribose 5-phosphate isomerase were characterized using reaction kinetics, resulting in calculated half-lives and Michaelis constants. Locally optimized reaction conditions were found through experimental modeling, resulting in apparent increases in the reaction rate of both of the enzymes. The enzyme ribose 5-phosphate isomerase was successfully encapsulated within polymersomes.

Subjects/Keywords: polymersome; phosphoribulokinase; optimization; ribose 5-phosphate isomerase; encapsulation; immobilization; enzyme; carbon capture

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nish, G. L. (2016). Optimization of the reaction conditions of two enzymes for use in a carbon sequestration process, and investigation into immobilization via encapsulation within polymersomes. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/cv405s966t

Chicago Manual of Style (16^th Edition):

Nish, Gordon L. “Optimization of the reaction conditions of two enzymes for use in a carbon sequestration process, and investigation into immobilization via encapsulation within polymersomes.” 2016. Masters Thesis, University of Alberta. Accessed January 16, 2021. https://era.library.ualberta.ca/files/cv405s966t.

MLA Handbook (7^th Edition):

Nish, Gordon L. “Optimization of the reaction conditions of two enzymes for use in a carbon sequestration process, and investigation into immobilization via encapsulation within polymersomes.” 2016. Web. 16 Jan 2021.

Vancouver:

Nish GL. Optimization of the reaction conditions of two enzymes for use in a carbon sequestration process, and investigation into immobilization via encapsulation within polymersomes. [Internet] [Masters thesis]. University of Alberta; 2016. [cited 2021 Jan 16]. Available from: https://era.library.ualberta.ca/files/cv405s966t.

Council of Science Editors:

Nish GL. Optimization of the reaction conditions of two enzymes for use in a carbon sequestration process, and investigation into immobilization via encapsulation within polymersomes. [Masters Thesis]. University of Alberta; 2016. Available from: https://era.library.ualberta.ca/files/cv405s966t

Universidade do Rio Grande do Sul

28. Lorenzoni, André Soibelmann Glock. Produção de fruto-oligossacarídeos e açúcar Invertido utilizando enzimas imobilizadas.

Degree: 2014, Universidade do Rio Grande do Sul

URL: http://hdl.handle.net/10183/96930

►

Fruto-oligossacarídeos (FOS) são fibras prebióticas com poder adoçante considerável, sendo um produto de alto valor para a indústria de alimentos. Açúcar invertido é o produto… (more)

▼

Fruto-oligossacarídeos (FOS) são fibras prebióticas com poder adoçante considerável, sendo um produto de alto valor para a indústria de alimentos. Açúcar invertido é o produto da hidrólise da sacarose possuindo maior poder adoçante, menor susceptibilidade à cristalização e maior higroscopicidade com relação à sacarose, sendo de grande interesse industrial. Ambos produtos podem ser produzidos por reações enzimáticas, utilizando β-frutosiltransferase e β- frutofuranosidase respectivamente, no entanto processos enzimáticos costumam ser caros devido ao alto custo e baixa estabilidade de enzimas. Esses fatores podem ser contornados com a imobilização da enzima, permitindo a reutilização e por vezes aumentando a estabilidade. No presente trabalho a enzima β-frutosiltransferase proveniente de um extrato comercial de Aspergillus aculeatus (Viscozyme L) foi parcialmente purificada, com resina de troca iônica, imobilizada covalentemente em esferas de quitosana e utilizada na produção de FOS. O processo de purificação aumentou a atividade específica em 6 vezes. A estabilidade do biocatalisador imobilizado foi avaliada em 50 bateladas para produção de FOS, foi observado cerca de 55 % de rendimento em cada batelada, sem perda de atividade detectada após as utilizações. Após esse experimento foi testada a utilização das esferas em reatores contínuos com leito fixo e fluidizado, com rendimentos de 59 % e 54 % respectivamente. A produção de açúcar invertido foi feita utilizando a enzima Maxinvert L (β-frutofuranosidase de Saccharomyces cerevisiae) que foi imobilizada, da mesma forma, em esferas de quitosana e sua utilização foi testada em reatores de leito fixo e fluidizado com rendimentos de 98 % e 94 % respectivamente. Os reatores de leito fixo possuem potencial para estudos envolvendo aplicações industriais tanto para produção de FOS quanto para produção de Açúcar Invertido.

Fructooligosaccharides (FOS) are prebiotic fibre with sweetening power, being a highvalue product for the food industry. Invert sugar is the product of sucrose hydrolysis; it has a higher sweetening power, it is less susceptible to crystallization and has a higher hygroscopicity than regular sugar. Finding many uses in food industry processes. Both products can be obtained by enzymatic reactions using β-fructosyltransferase and β- fructofuranosidase, respectively. However, enzymatic processes are often costly because of high enzymatic cost and lack of operational stability. These drawbacks can be overcome by immobilization of enzyme, enabling reuses and usually increasing its stability. In the present work, β-fructofuranosidase from a commercial preparation from Aspergillus aculeatus (Viscozyme L) was partially purified, covalently immobilized on chitosan spheres and used for FOS production. Partial purification resulted in a 6-fold increase in specific activity. Operational stability of biocatalyst was evaluated along 50 batches, resulting in around 55 % yield on each batch and no loss of activity after batches. The immobilized biocatalyst was also used…

Advisors/Committee Members: Hertz, Plinho Francisco.

Subjects/Keywords: Enzyme immobilization; Quitosana; Enzimas imobilizadas; Enzymatic reactors; Fructooligosaccharides; Açúcar invertido; Invert sugar; Frutooligossacarídeo; Chitosan

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lorenzoni, A. S. G. (2014). Produção de fruto-oligossacarídeos e açúcar Invertido utilizando enzimas imobilizadas. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/96930

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lorenzoni, André Soibelmann Glock. “Produção de fruto-oligossacarídeos e açúcar Invertido utilizando enzimas imobilizadas.” 2014. Thesis, Universidade do Rio Grande do Sul. Accessed January 16, 2021. http://hdl.handle.net/10183/96930.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lorenzoni, André Soibelmann Glock. “Produção de fruto-oligossacarídeos e açúcar Invertido utilizando enzimas imobilizadas.” 2014. Web. 16 Jan 2021.

Vancouver:

Lorenzoni ASG. Produção de fruto-oligossacarídeos e açúcar Invertido utilizando enzimas imobilizadas. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2014. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/10183/96930.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lorenzoni ASG. Produção de fruto-oligossacarídeos e açúcar Invertido utilizando enzimas imobilizadas. [Thesis]. Universidade do Rio Grande do Sul; 2014. Available from: http://hdl.handle.net/10183/96930

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

UCLA

29. Koo, Bonhye. Micro and Nanoscale Fabrication and Characterization For Next-Generation Biosensors.

Degree: Chemical Engineering, 2016, UCLA

URL: http://www.escholarship.org/uc/item/7ht0j7h4

► Pressing performance demands require next-generation biosensors to detect target chemical and biological molecules with higher sensitivity, shorter response times, and lower detection limit. Micro- and… (more)

▼ Pressing performance demands require next-generation biosensors to detect target chemical and biological molecules with higher sensitivity, shorter response times, and lower detection limit. Micro- and nanoscale devices are attractive for a wide range of biosensor applications since at small scale, in addition to being more compact, the device may exhibit improved performance. The benefits include minimization of tissue damage for implantable devices, improved spatial resolution and sensitivity, as well as increased surface charge to mass ratio, which is important for the performance of our novel technology for nucleic acid detection described below. Borrowing from the processing technologies used in the semiconductor industry, we implemented micromachining techniques to fabricate devices at both the micro- and nanoscale. In this dissertation, we present our work on the fabrication and characterization of two next-generation biosensors. The first device we fabricated is a sequence-specific nucleic acid sensor based on the blockage of a nanopore. Current methods for nucleic acid detection generally rely on polymerase chain reaction (PCR) and fluorescent labeling, however, these methods render the devices slow, expensive, complex, and bulky. In order to address these limitations, a new sensor was fabricated from a single glass wafer, consisting of a glass nanopore in a thin glass membrane. For nanopore sensing, low frequency noise is critical since it limits the discrimination of signal change based on target analyte movement from the fluctuation of noise. To further our understanding of nanopores, we observed how different pore geometries affect noise characteristics, and then compared this newly developed glass nanopore to conventional Si-based nanopores. Based on the analysis, low-noise glass nanopores, suitable for sequence-specific nucleic acid detection, were fabricated. By scaling down the pore diameter to the nano-regime, 1 aM detection of 16S rRNA from Escherichia coli was demonstrated even in the presence of a million-fold background of RNA from Pseudomona putida. This new platform for the PCR-free, optics-free, label-free sequence-specific nucleic acid detection shows the potential to detect pathogens in body fluids, food, or water. In addition, we developed a new method to transfer enzyme to a microelectrode array on an implantable microprobe, which enables fabrication of better performing microprobes for the sensing of multiple neurochemicals in vivo. Monitoring the release of neurotransmitters in real-time offers valuable information necessary to understand neurological disorders and abnormal behaviors. We employed polydimethylsiloxane (PDMS) stamping to transfer enzyme onto microelectrode array microprobes. A model enzyme, glucose oxidase (GOx), was stamped onto the surface of disk electrodes to test the feasibility of PDMS stamping for biosensor fabrication. The model sensor showed a good combination of performance (29 μA/mM cm2 sensitivity and 4 μM detection limit) proving that PDMS stamping offers a…

Subjects/Keywords: Chemical engineering; Biomedical engineering; biosensor; enzyme immobilization; low noise; microcontact printing; nanopore; nucleic acid detection

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Koo, B. (2016). Micro and Nanoscale Fabrication and Characterization For Next-Generation Biosensors. (Thesis). UCLA. Retrieved from http://www.escholarship.org/uc/item/7ht0j7h4

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Koo, Bonhye. “Micro and Nanoscale Fabrication and Characterization For Next-Generation Biosensors.” 2016. Thesis, UCLA. Accessed January 16, 2021. http://www.escholarship.org/uc/item/7ht0j7h4.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Koo, Bonhye. “Micro and Nanoscale Fabrication and Characterization For Next-Generation Biosensors.” 2016. Web. 16 Jan 2021.

Vancouver:

Koo B. Micro and Nanoscale Fabrication and Characterization For Next-Generation Biosensors. [Internet] [Thesis]. UCLA; 2016. [cited 2021 Jan 16]. Available from: http://www.escholarship.org/uc/item/7ht0j7h4.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Koo B. Micro and Nanoscale Fabrication and Characterization For Next-Generation Biosensors. [Thesis]. UCLA; 2016. Available from: http://www.escholarship.org/uc/item/7ht0j7h4

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade do Rio Grande do Sul

30. Rodrigues, Rafael Costa. Síntese de biodiesel através de transesterificação enzimática de óleos vegetais catalisada por lipase imobilizada por ligação covalente multipontual.

Degree: 2009, Universidade do Rio Grande do Sul

URL: http://hdl.handle.net/10183/15447

► Biodiesel consiste de ésteres alquílicos de ácidos graxos produzidos pela transesterificação de triglicerídeos com álcoois de cadeia curta. Tradicionalmente, a reação ocorre na presença de… (more)

▼ Biodiesel consiste de ésteres alquílicos de ácidos graxos produzidos pela transesterificação de triglicerídeos com álcoois de cadeia curta. Tradicionalmente, a reação ocorre na presença de catalisadores químicos, como álcalis ou ácidos. A utilização alternativa de lipases como biocatalisadores na reação de síntese do biodiesel não gera materiais residuais tóxicos, e o glicerol pode ser facilmente recuperado sem um processamento complexo. Neste trabalho estudou-se a síntese de biodiesel através da transesterificação enzimática de óleos vegetais e álcoois de cadeia curta catalisada pela lipase de Thermomyces lanuginosus (TLL). Primeiramente, as condições da reação de transesterificação, entre óleo de soja e etanol catalisada pela TLL em sua forma livre foram otimizadas através de planejamento experimental e da metodologia de superfície de resposta. A seguir, foram estudadas as condições para a reação catalisada pela TLL imobilizada comercial (Lipozyme TL-IM). Na etapa seguinte foram avaliados diferentes óleos vegetais (óleo de soja, óleo de girassol e óleo de arroz) e álcoois (metanol, etanol, propanol e butanol), na reação de transesterificação catalisada por três derivados enzimáticos comerciais de lipases de diferentes fontes (Lipozyme TL-IM, Novozym 435, Lipozyme RMIM), onde se verificou que cada lipase apresentou uma especificidade diferente em relação ao álcool preferente para a reação de transesterificação. Além disso, a reação utilizando diferentes óleos vegetais apresentou resultados semelhantes, mostrando ser este um fator mais dependente do ponto de vista econômico que processual. Também se estudou diferentes tratamentos para melhorar o reuso da lipase imobilizada, onde a lavagem do derivado com n-hexano, após cada ciclo, manteve a atividade da lipase em torno de 90% da atividade inicial após sete ciclos. Prosseguindo, foi estudada a imobilização e estabilização da TLL através da ligação covalente multipontual em suporte glioxil-agarose. Para obter isto se procedeu a aminação química da superfície da enzima, o que permitiu a obtenção de um derivado com alto grau de imobilização e alta estabilidade quanto à inativação por temperatura e solventes orgânicos. A seguir avaliou-se a possibilidade de reativação de derivados submetidos a processos de inativação. Em um primeiro momento os estudos foram realizados com derivados de TLL imobilizada unipontualmente em agarose ativada com brometo de cianogênio, onde foi possível obter a reativação completa do derivado inativado por solventes orgânicos através de incubação em meio aquoso. Verificou-se também que um dos principais pontos que dificultam a reativação de lipases é a recuperação do mecanismo de abertura do lid, que é facilitado com a presença de detergentes. Logo, estudos de reativação com derivados imobilizados por ligação covalente multipontual, mostraram que este tipo de imobilização auxilia no processo de reativação por oferecer mais pontos de referência no momento da reativação. A etapa final deste trabalho foi a aplicação do derivado de TLL imobilizado… Advisors/Committee Members: Ayub, Marco Antônio Záchia.

Subjects/Keywords: Ethanolysis; Biodiesel; Vegetable oils; Óleos vegetais; Experimental design; Lipase; Multipoint immobilization; Enzyme stability

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rodrigues, R. C. (2009). Síntese de biodiesel através de transesterificação enzimática de óleos vegetais catalisada por lipase imobilizada por ligação covalente multipontual. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/15447

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rodrigues, Rafael Costa. “Síntese de biodiesel através de transesterificação enzimática de óleos vegetais catalisada por lipase imobilizada por ligação covalente multipontual.” 2009. Thesis, Universidade do Rio Grande do Sul. Accessed January 16, 2021. http://hdl.handle.net/10183/15447.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rodrigues, Rafael Costa. “Síntese de biodiesel através de transesterificação enzimática de óleos vegetais catalisada por lipase imobilizada por ligação covalente multipontual.” 2009. Web. 16 Jan 2021.

Vancouver:

Rodrigues RC. Síntese de biodiesel através de transesterificação enzimática de óleos vegetais catalisada por lipase imobilizada por ligação covalente multipontual. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2009. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/10183/15447.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rodrigues RC. Síntese de biodiesel através de transesterificação enzimática de óleos vegetais catalisada por lipase imobilizada por ligação covalente multipontual. [Thesis]. Universidade do Rio Grande do Sul; 2009. Available from: http://hdl.handle.net/10183/15447

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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Open Access Theses and Dissertations