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Universidade de Lisboa
1.
Neto, Bruno Semedo.
Sistema de arquivos de documentos digitais.
Degree: 2011, Universidade de Lisboa
URL: http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ul.pt:10451/15269 
► This thesis reports on the project developed between September 2010 and May 2011 at Deloitte Portugal, to pursuit a master’s degree in Computer Science, specialization…
(more)
▼ This thesis reports on the project developed between September 2010 and May 2011 at Deloitte Portugal, to pursuit a master’s degree in Computer Science,
specialization in information systems at Faculdade de Ciências da Universidade de
Lisboa.
The internship aimed at the development and integration of an
ECM – Enterprise
Content Management module, to the company’s purchasing department based on OpenText
ECM. For that, it was developed a portfolio of digital purchases for the department in order to allow easier access to digital documents, to scanned contracts and to search for information based on criteria defined by the purchasing department. This
purchase folder was developed as a module for the D:Files system, which is used in the
company to manage documents.
We used the used the scrum methodology, which is the one that best suits the
characteristics of the project, because it was necessary to present and evaluate the new
module to the end users. The results met the needs listed by the department, and even
exceeded them since it was possible to develop a system with a level of interaction higher than the expected by the purchases department of Deloitte Portugal.
The internship, besides the objective of establishing the purchase folder, also served
to deepen and apply the knowledge acquired during graduation and master's degrees in
computer science, particularly the design, the development, and the project management,
as well as to increase the capacity for self-learning, time management, interpersonal
relationships, and understanding of the business world.
On the internship it was performed additional work not planned on the original
project, such as the user support and the participation in evolutionary maintenance
system. So it was possible to get a continuous training on the entire D:Files project and also get an extended training on the D:Files system.
The trainee also developed other projects, such as the centralization of digital
information for the consulting department in the LiveLink system, the creation of Web
services for use in other areas, the creation of informational web pages, the testing of D:Files system modules, the development of a system to store and deliver large files that
x where previously handled in paper format, the system configuration used in the business,and the development of a system to control the company's budget.
Advisors/Committee Members: Martins, Francisco, Furtado, Rosa Isabel Pestana.
Subjects/Keywords: ECM; D:Files; OpenText
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APA (6th Edition):
Neto, B. S. (2011). Sistema de arquivos de documentos digitais. (Thesis). Universidade de Lisboa. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ul.pt:10451/15269
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Neto, Bruno Semedo. “Sistema de arquivos de documentos digitais.” 2011. Thesis, Universidade de Lisboa. Accessed December 10, 2019.
http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ul.pt:10451/15269.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Neto, Bruno Semedo. “Sistema de arquivos de documentos digitais.” 2011. Web. 10 Dec 2019.
Vancouver:
Neto BS. Sistema de arquivos de documentos digitais. [Internet] [Thesis]. Universidade de Lisboa; 2011. [cited 2019 Dec 10].
Available from: http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ul.pt:10451/15269.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Neto BS. Sistema de arquivos de documentos digitais. [Thesis]. Universidade de Lisboa; 2011. Available from: http://www.rcaap.pt/detail.jsp?id=oai:repositorio.ul.pt:10451/15269
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Manchester
2.
Randles, Michael.
Proteomic analyses of kidney glomerular extracellular matrix in health and disease.
Degree: PhD, 2015, University of Manchester
URL: https://www.research.manchester.ac.uk/portal/en/theses/proteomic-analyses-of-kidney-glomerular-extracellular-matrix-in-health-and-disease(a39fe408-db06-4d80-b97b-4e0651bf7bc3).html
;
http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.634958
► Glomerular filtration is a vital physiological process removing waste products from the circulation and this process occurs across the glomerular filtration barrier (GFB). The cells…
(more)
▼ Glomerular filtration is a vital physiological process removing waste products from the circulation and this process occurs across the glomerular filtration barrier (GFB). The cells and extracellular matrix (ECM), which form this barrier, are exposed to forces during ultrafiltration and special adaptation is required to withstand these forces. Dysfunction in cellular adhesion machinery or ECM assembly within the GFB causes loss of selective glomerular filtration, however, the mechanisms governing these processes are poorly understood. To this end we sought to characterise the glomerular ECM and adhesion machinery using high throughput mass spectrometry (MS)-based proteomics. MS of human glomerular ECM identified a highly complex extracellular niche, revealing the potential involvement of novel ECM proteins in glomerular development and disease processes. Furthermore we identified that glomerular cells in culture had distinct ECM proteomes and interestingly, coculture experiments demonstrated that the ECM proteome was influenced by cellular crosstalk and had a closer resemblance to glomerular ECM in vivo. Protein network analyses of in vivo and in vitro ECM datasets revealed a common core of highly connected structural ECM proteins that may be important for glomerular ECM assembly. To understand how this ECM proteome altered in disease, we studied mice with mild glomerular dysfunction. Here, transcriptomic and proteomic analyses identified alterations in ECM composition and 3D electron microscopy revealed striking ultrastructural changes in glomerular ECM. MS-based proteomics was next applied to the analysis of glomerular podocyte adhesion complexes, leading to the discovery that the actin cytoskeletal regulators and trafficking machinery are recruited to adhesions sites in an ECM-ligand dependent manner. Furthermore, these differences functionally altered cell shape and adhesion strength. These same analyses were applied to podocyte cell-cell junctions, revealing an unexpected overlap of cell-ECM and cell-cell adhesion machinery. Overall, these findings demonstrate for the first time the complexity of the glomerular ECM and adhesion signalling complexes and reinforce the benefits of global, unbiased experimental approaches. In addition the results suggest that glomerular ECM composition, organisation and adhesion signalling are context dependent, and therefore, represent potential therapeutic targets.
Subjects/Keywords: 616.6; ECM; Proteomics
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APA (6th Edition):
Randles, M. (2015). Proteomic analyses of kidney glomerular extracellular matrix in health and disease. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/proteomic-analyses-of-kidney-glomerular-extracellular-matrix-in-health-and-disease(a39fe408-db06-4d80-b97b-4e0651bf7bc3).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.634958
Chicago Manual of Style (16th Edition):
Randles, Michael. “Proteomic analyses of kidney glomerular extracellular matrix in health and disease.” 2015. Doctoral Dissertation, University of Manchester. Accessed December 10, 2019.
https://www.research.manchester.ac.uk/portal/en/theses/proteomic-analyses-of-kidney-glomerular-extracellular-matrix-in-health-and-disease(a39fe408-db06-4d80-b97b-4e0651bf7bc3).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.634958.
MLA Handbook (7th Edition):
Randles, Michael. “Proteomic analyses of kidney glomerular extracellular matrix in health and disease.” 2015. Web. 10 Dec 2019.
Vancouver:
Randles M. Proteomic analyses of kidney glomerular extracellular matrix in health and disease. [Internet] [Doctoral dissertation]. University of Manchester; 2015. [cited 2019 Dec 10].
Available from: https://www.research.manchester.ac.uk/portal/en/theses/proteomic-analyses-of-kidney-glomerular-extracellular-matrix-in-health-and-disease(a39fe408-db06-4d80-b97b-4e0651bf7bc3).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.634958.
Council of Science Editors:
Randles M. Proteomic analyses of kidney glomerular extracellular matrix in health and disease. [Doctoral Dissertation]. University of Manchester; 2015. Available from: https://www.research.manchester.ac.uk/portal/en/theses/proteomic-analyses-of-kidney-glomerular-extracellular-matrix-in-health-and-disease(a39fe408-db06-4d80-b97b-4e0651bf7bc3).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.634958
University of Alberta
3.
Kandalam, Vijay S.
The Role of TIMPs in Heart Disease.
Degree: PhD, Department of Physiology, 2012, University of Alberta
URL: https://era.library.ualberta.ca/files/08612n98z
► Heart disease is a leading cause of morbidity and mortality in the world with a growing prevalence in a variety of manifestations. Many of the…
(more)
▼ Heart disease is a leading cause of morbidity and
mortality in the world with a growing prevalence in a variety of
manifestations. Many of the events that occur in the heart during
the progression of disease have been explored to identify a
causative mechanism to develop effective treatments and possibly a
cure for heart disease. There is a growing body of evidence
demonstrating the necessity for adaptive remodeling of the
extracellular matrix (ECM) for proper cardiac function in response
to disease through balanced regulation of its structure. Cardiac
ECM serves as a structural framework for the myocardium, and its
integrity is maintained by the function of matrix
metalloproteinases (MMPs) which are kept under control by their
physiological inhibitors, Tissue Inhibitor of Metalloproteinases
(TIMPs). A balance between the MMPs and TIMPs is important for
optimal degradation and replacement of the ECM either in
physiological ECM turnover or in adverse ECM remodeling in disease.
The research presented in this thesis consists of our investigation
of the role of two highly expressed TIMPs in the heart, TIMP2 and
TIMP3, in response to two common models of human heart disease.
Mice lacking TIMP2 or TIMP3 were subjected to myocardial
infarction, while the outcome of TIMP2 deficiency was also studied
in response to cardiac pressure overload. Myocardial infarction led
to different outcomes in the TIMP2-/- and TIMP3-/- mice. While
TIMP3-/- mice exhibited elevated rates of left ventricular rupture
incidence and severely compromised survival, as well as significant
cardiac dysfunction, lack of TIMP2 exacerbated cardiac function
without compromising survival compared to parallel wildtype mice.
Following pressure overload, TIMP2-/- mice showed left ventricular
dilation and dysfunction, hypertrophy and fibrosis which we found
to be linked to impaired ECM-myocyte connection due to excess
degradation of integrin-1 via excess activity of membrane-type 1
MMP (MT1-MMP). We have therefore identified important and distinct
roles for these TIMPs in heart disease. The results presented in
this thesis are important contributions to the study of the role of
ECM remodeling in the adaptive cardiac response to injury, as
demonstrated through a wide variety of physiological, histological,
and molecular analyses of the development of heart disease in each
model. These findings provide novel evidence identifying distinct
mechanisms or pattern of events associated with TIMP2 and TIMP3
that can alter the current understanding of the role of each TIMP
in the development and progression of heart disease.
Subjects/Keywords: TIMP; ECM; disease; MMP; heart
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APA (6th Edition):
Kandalam, V. S. (2012). The Role of TIMPs in Heart Disease. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/08612n98z
Chicago Manual of Style (16th Edition):
Kandalam, Vijay S. “The Role of TIMPs in Heart Disease.” 2012. Doctoral Dissertation, University of Alberta. Accessed December 10, 2019.
https://era.library.ualberta.ca/files/08612n98z.
MLA Handbook (7th Edition):
Kandalam, Vijay S. “The Role of TIMPs in Heart Disease.” 2012. Web. 10 Dec 2019.
Vancouver:
Kandalam VS. The Role of TIMPs in Heart Disease. [Internet] [Doctoral dissertation]. University of Alberta; 2012. [cited 2019 Dec 10].
Available from: https://era.library.ualberta.ca/files/08612n98z.
Council of Science Editors:
Kandalam VS. The Role of TIMPs in Heart Disease. [Doctoral Dissertation]. University of Alberta; 2012. Available from: https://era.library.ualberta.ca/files/08612n98z
Texas A&M University
4.
Kwak, Hyo Bum.
Exercise training regulation of extracellular matrix and remodeling in the aging rat heart.
Degree: 2009, Texas A&M University
URL: http://hdl.handle.net/1969.1/ETD-TAMU-2761
► Aging is characterized by a progressive impairment of cardiac structure and function. The cardiac remodeling involves loss of cardiac myocytes, reactive hypertrophy of the remaining…
(more)
▼ Aging is characterized by a progressive impairment of cardiac structure and
function. The cardiac remodeling involves loss of cardiac myocytes, reactive
hypertrophy of the remaining cells, and increased extracellular matrix (
ECM) and
fibrosis in the aging heart. In contrast, exercise training not only improves cardiac
function, but also reduces the risk of heart disease. However, the ability of exercise
training to modulate
ECM and remodeling in the aging heart remains unknown.
Therefore, the purpose of this study was to determine the effects of exercise training on
ECM remodeling in the aging heart. We hypothesized that (1) exercise training would
attenuate age-related changes in left ventricle morphology including extramyocyte space
and collagen contents, and (2) exercise training would ameliorate age-induced changes
in
ECM-related factors including MMPs, TIMPs, TNF-?, TGF-?1, and ?-SMA in the
heart. Three and 31 month old Fischer 344 ? Brown Norway F1 hybrid rats were
assigned to four groups: young sedentary (YS), young exercise-trained (YE), old sedentary (OS), and old exercise-trained (OE). Exercise training groups walked briskly
on a treadmill for 45 min/day (12? incline) at 20m/min (young) or 10 m/min (old), 5
d/wk for 12 wk. We found that endurance exercise training might ameliorate the ageinduced
increase in extramyocyte space and collagen contents of the left ventricle.
Exercise training might protect against age-induced fibrosis by increasing MMP-2,
MMP-14 in the soluble fraction and MMP-1, MMP-3, MMP-14 in the insoluble fraction
of old rat hearts. Conversely, exercise training might reduce the fibrosis by decreasing
TIMP-1 in the soluble fraction of old rat hearts. Further, exercise training reduced
potential upstream pro-fibrotic mediators including TNF-? and TGF-?1 in the aging rat
hearts. These results are the first to demonstrate that exercise training has a protective
effect against age-induced extracellular collagen matrix remodeling in the aging heart,
associated with increased MMP-1, -2, -3, -14 and decreased TIMP-1, TNF-?, and TGF-
?1.
Advisors/Committee Members: Lawler, John M. (advisor), Fluckey, James D. (committee member), Hood, David M. (committee member), Riechman, Steven E. (committee member).
Subjects/Keywords: aging; exercise; ECM; heart
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APA (6th Edition):
Kwak, H. B. (2009). Exercise training regulation of extracellular matrix and remodeling in the aging rat heart. (Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2761
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Kwak, Hyo Bum. “Exercise training regulation of extracellular matrix and remodeling in the aging rat heart.” 2009. Thesis, Texas A&M University. Accessed December 10, 2019.
http://hdl.handle.net/1969.1/ETD-TAMU-2761.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Kwak, Hyo Bum. “Exercise training regulation of extracellular matrix and remodeling in the aging rat heart.” 2009. Web. 10 Dec 2019.
Vancouver:
Kwak HB. Exercise training regulation of extracellular matrix and remodeling in the aging rat heart. [Internet] [Thesis]. Texas A&M University; 2009. [cited 2019 Dec 10].
Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2761.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Kwak HB. Exercise training regulation of extracellular matrix and remodeling in the aging rat heart. [Thesis]. Texas A&M University; 2009. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2761
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Debrecen
5.
Csehy, Ladislav.
Alfresco
.
Degree: DE – TEK – Informatikai Kar, 2009, University of Debrecen
URL: http://hdl.handle.net/2437/85689
Alfresco egy céges tartalomkezeő szoftver, mely adatok kezelésére, rendezésére, keresésére, publikálására és még nagyon sok mindenre használható. Egy nagyon komplex csomag, melyet a nyít forráskódban készítettek el, és a legújabb Internetes technológiákat sorakozatja fel munkája sikeréért.
Advisors/Committee Members: Boda, István (advisor).Subjects/Keywords: dokumentumkezelő;
tartalomkezelő;
ECM;
nyílt forráskód
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APA (6th Edition):
Csehy, L. (2009). Alfresco
. (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/85689
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Csehy, Ladislav. “Alfresco
.” 2009. Thesis, University of Debrecen. Accessed December 10, 2019.
http://hdl.handle.net/2437/85689.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Csehy, Ladislav. “Alfresco
.” 2009. Web. 10 Dec 2019.
Vancouver:
Csehy L. Alfresco
. [Internet] [Thesis]. University of Debrecen; 2009. [cited 2019 Dec 10].
Available from: http://hdl.handle.net/2437/85689.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Csehy L. Alfresco
. [Thesis]. University of Debrecen; 2009. Available from: http://hdl.handle.net/2437/85689
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Waterloo
6.
Wu, Zhiping.
Data Structures for Fast Access Control in ECM Systems.
Degree: 2014, University of Waterloo
URL: http://hdl.handle.net/10012/8614
► While many access control models have been proposed, little work has been done on the efficiency of access control systems. Because the access control sub-system…
(more)
▼ While many access control models have been proposed, little work has been done on the efficiency of access control systems. Because the access control sub-system of an Enterprise Content Management (ECM) system may be a bottleneck, we investigate the representation of permissions to improve its efficiency. Observing that there are many browsing-oriented permission request queries, we choose to implement a subject-oriented representation (i.e., maintaining a permission list for each subject). Additionally, we notice that with breadth-first ID numbering we may encounter many contiguous IDs under one object (e.g., folder) .
To optimize the efficiency taking into account the above two characteristics, this thesis presents a space-efficient data structure specifically tailored for representing permission lists in ECM systems. Besides the space efficiency, checking, granting or revocation of a permission is very fast using our data structure. It also supports fast union of two or more permission lists (determining the effective permissions inherited from users' groups). In addition, our data structure is scalable to support any increase in the number of objects and subjects.
We evaluate our representation by comparing it against the bitmap based representation and a hash table based representation while using random ID numbering and breadth-first numbering, respectively. Our experimental tests on both synthetic and real-world data show that the hash table outperforms our representation for regular permission queries (i.e., querying permissions on a single object each time) as well as browsing-oriented queries with random ID numbering. However, our tests also show that 1) our representation supports faster browsing-oriented queries with breadth-first ID numbering applied while consuming only half the space when compared to the hash table based representation, and 2) our representation is much more space and time efficient than the bitmap based representation for our application.
Subjects/Keywords: Access Control; ECM System
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APA (6th Edition):
Wu, Z. (2014). Data Structures for Fast Access Control in ECM Systems. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/8614
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Wu, Zhiping. “Data Structures for Fast Access Control in ECM Systems.” 2014. Thesis, University of Waterloo. Accessed December 10, 2019.
http://hdl.handle.net/10012/8614.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Wu, Zhiping. “Data Structures for Fast Access Control in ECM Systems.” 2014. Web. 10 Dec 2019.
Vancouver:
Wu Z. Data Structures for Fast Access Control in ECM Systems. [Internet] [Thesis]. University of Waterloo; 2014. [cited 2019 Dec 10].
Available from: http://hdl.handle.net/10012/8614.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Wu Z. Data Structures for Fast Access Control in ECM Systems. [Thesis]. University of Waterloo; 2014. Available from: http://hdl.handle.net/10012/8614
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
7.
Möller, Laura Artoni.
Interação celular na placenta de gestações de bovinos clonados com especial ênfase às integrinas.
Degree: PhD, Anatomia dos Animais Domésticos e Silvestres, 2009, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/10/10132/tde-02122009-091433/
;
► Integrinas são glicoproteínas transmembrânicas envolvidas na adesão célula-célula e célula-proteina da matriz extracellular (ECM) que participam da migração e fusão de células trofoblásticas gigantes (TGC)…
(more)
▼ Integrinas são glicoproteínas transmembrânicas envolvidas na adesão célula-célula e célula-proteina da matriz extracellular (
ECM) que participam da migração e fusão de células trofoblásticas gigantes (TGC) com células do epitélio materno no placentoma bovino e interagem com inibidores teciduais de metalloproteinases (TIMPs), considerados importantes reguladores das metaloproteinases de matriz (MMPs), que são reconhecidas como passo limitande para ação de enzimas que atuam no remodelamento da
ECM na implantação e na gestação. Uma vez que as integrinas são consideradas responsáveis pela manutenção da arquitetura tecidual e que as MMPs podem regular a degradação e a reconstituição da
ECM necessária para a invasão do trofoblasto, temos como objetivo verificar uma possível relação das integrinas com alterações placentárias comumente observadas em gestações de animais clonados (SCNT). Para avaliar a funcionalidade placentária, a expressão do lactogênio placentário (PL) e do antígeno Ki-67 foram também verificadas. Placentomas bovinos foram coletados e divididos em 3 grupos: 1) início (n = 6) e 2) final (n = 3) da gestação de animais derivados de monta natural (n=9) e final da gestação de animais clonados (n=8), clones machos (n=4) e clones fêmeas (n=4). As proteínas foram localizadas por imuno-histoquímica indireta, exceto as subunidades de integrina α6, β1, αV e β3 que foram localizadas por imunofluorescência. A especificidade dos anticorpos e expressão protéica foi confirmada por Western blot e a expressão do RNAm foi avaliada por meio de RT-PCR e PCR em tempo real quantitativo. As células positivas para a laminina, TIMP-2, Ki-67 e lactogênio placentário (PL) foram quantificadas e para comparação entre os grupos. A proteína das subunidades α6 do receptor de integrina e β1 foi observada nos animais clonados e não clonados nos estromas materno e fetal e porção basal dos epitélios materno e fetal e foi co-localizada com a laminina. A proteína das subunidades αV e β3 do receptor de integrina foi observado nos estromas materno e fetal e foi co-localizado com a fibronectina. O TIMP-2 foi exclusivamente e especificamente localizado nas TGCs no início e final da gestação de animais clonados e não clonados, mas não houve diferença significativa em sua expressão. O PL apresentou a mesma localização do TIMP-2 em todos os grupos analisados. Houve diferença significativa (p<0,05) entre 270d e clones ao se comprar a expressão relativa do RNAm da subunidade de integrina β1 e do lactogênio placentário e ao se comparar a expressão protéica por western blot da laminina e do TIMP-2. Foi possível observar diferença significativa (p<0,05) entre o grupo controle e o grupo de animais clonados ao se quantificar o número de TGCs positivas para a laminina e para o lactogênio placentário. Apesar de algumas diferenças terem sido observadas na expressão de integrinas e de proteínas da
ECM, sugere-se que a interação das integrinas com seus ligantes da
ECM a termo não é um determinante da sobrevivência…
Advisors/Committee Members: Papa, Paula de Carvalho.
Subjects/Keywords: ECM; ECM; Integrinas; Integrins; Placenta; Placenta; SCNT; SCNT; TIMPs; TIMPs
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APA ·
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Manager
APA (6th Edition):
Möller, L. A. (2009). Interação celular na placenta de gestações de bovinos clonados com especial ênfase às integrinas. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/10/10132/tde-02122009-091433/ ;
Chicago Manual of Style (16th Edition):
Möller, Laura Artoni. “Interação celular na placenta de gestações de bovinos clonados com especial ênfase às integrinas.” 2009. Doctoral Dissertation, University of São Paulo. Accessed December 10, 2019.
http://www.teses.usp.br/teses/disponiveis/10/10132/tde-02122009-091433/ ;.
MLA Handbook (7th Edition):
Möller, Laura Artoni. “Interação celular na placenta de gestações de bovinos clonados com especial ênfase às integrinas.” 2009. Web. 10 Dec 2019.
Vancouver:
Möller LA. Interação celular na placenta de gestações de bovinos clonados com especial ênfase às integrinas. [Internet] [Doctoral dissertation]. University of São Paulo; 2009. [cited 2019 Dec 10].
Available from: http://www.teses.usp.br/teses/disponiveis/10/10132/tde-02122009-091433/ ;.
Council of Science Editors:
Möller LA. Interação celular na placenta de gestações de bovinos clonados com especial ênfase às integrinas. [Doctoral Dissertation]. University of São Paulo; 2009. Available from: http://www.teses.usp.br/teses/disponiveis/10/10132/tde-02122009-091433/ ;
Johannes Gutenberg Universität Mainz
8.
Vasel, Matthäus.
Untersuchungen zu der Rolle der extrazellulären Matrix bei Fibrose und bei Tumoren.
Degree: 2012, Johannes Gutenberg Universität Mainz
URL: http://ubm.opus.hbz-nrw.de/volltexte/2012/3241/
► Diese Arbeit befasst sich mit der Rolle der extrazellulären Matrix und insbesondere des Proteins Fibronektin bei der Leberfibrose und bei der Einnistung von Tumorzellen in…
(more)
▼ Diese Arbeit befasst sich mit der Rolle der extrazellulären Matrix und insbesondere des Proteins Fibronektin bei der Leberfibrose und bei der Einnistung von Tumorzellen in die Stammzellnische im Knochenmark.rnrnHierfür wurde in einem Fibrosemodell das Peptid pUR4b verwendet, welches die Assemblierung einer Fibronektinmatrix verhindert. Bei der Verwendung dieses Peptids während und nach der Induktion einer Leberfibrose durch die Chemikalie Dimethylnitrosamin konnte eine Verminderung der Kollagenmenge (und damit des fibrotischen Narbengewebes) in der Leber im Vergleich zu fibrotischen Kontrolltieren beobachtet werden. Darüber hinaus konnte gezeigt werden, dass dieser Effekt unabhängig von der Aktivierung der hepatischen Stellatezellen ist, jedoch zum Teil von einer verminderten Anzahl entzündlicher Zellen abhängig sein könnte. Eine verminderte Bildung von Gesamt- und aktivem TGF-β, welche zum Teil auf Effekte der verringerten Zahl der inflammatorischen Zellen zurückzuführen sein könnte, unterstützt den Effekt des verminderten Aufbaus von Narbengewebe. In vitro Untersuchungen zeigten, dass hepatische Stellatezellen bei einer Behandlung mit pUR4b weniger Fibronektin in die extrazelluläre Matrix einbauten als unbehandelte hepatische Stellatezellen. Insgesamt sprechen die Daten dafür, dass das Peptid pUR4b den Aufbau einer Fibronektinmatrix verhinderte bzw. verminderte, wodurch die Ablagerung anderer Komponenten der extrazellulären Matrix wie z.B. Kollagen gestört war und es daher zu einem Rückgang des fibrotischen Narbengewebes kam.rnrnFür die Untersuchung des Einflusses der extrazellulären Matrix und des Fibronektins bei der Einnistung von Tumorzellen wurde zunächst das Fibronektin mit Hilfe konditioneller Knockout-Mäuse in verschiedenen Zellen bzw. Organen der Tiere ausgeschaltet. Weder die Ausschaltung des zirkulierenden, noch des durch Osteoblasten und Osteozyten gebildeten, noch des zirkulierenden und von Zellen des Knochenmarks gebildeten Fibronektins beeinträchtigte die Einnistung von Tumorzellen. Auch die Bildung eines Hämatoms im Knochen hatte weder einen Einfluss auf die Einnistung von Tumorzellen noch auf die spätere Tumorentwicklung. Die Ausschaltung des tumorzellendogenen Fibronektins führte hingegen zu einer signifikant verminderten Einnistung von Tumorzellen. Diese ist wahrscheinlich auf die verstärkte Affinität dieser Tumorzellen zu Zellen des Immunsystems zurückzuführen. Diese Beobachtung konnte zum Teil durch eine verstärkte eCadherin Expression erklärt werden, welche die Bindung an verschiedene Zellen des Immunsystems vermittelt. Eine Untersuchung der osteoblastischen Stammzellnische durch die kombinierte Gabe von Parathormon und Zoledronsäure führte zu keiner Veränderung der Fibronektinkonzentration innerhalb des Knochenmarks der behandelten Tiere. Dennoch nisteten sich in dem Knochenmark der mit Parathormon und Zoledronsäure behandelten Tiere signifikant mehr Tumorzellen ein als in dem von Kontrolltieren. Dieser Effekt konnte auf einen synergetischen Effekt von Parathormon und Zoledronsäure…
Subjects/Keywords: Fibronektin, extrazelluläre Matrix, ECM; fibronectin, extracellular matrix, ECM; Life sciences
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APA (6th Edition):
Vasel, M. (2012). Untersuchungen zu der Rolle der extrazellulären Matrix bei Fibrose und bei Tumoren. (Doctoral Dissertation). Johannes Gutenberg Universität Mainz. Retrieved from http://ubm.opus.hbz-nrw.de/volltexte/2012/3241/
Chicago Manual of Style (16th Edition):
Vasel, Matthäus. “Untersuchungen zu der Rolle der extrazellulären Matrix bei Fibrose und bei Tumoren.” 2012. Doctoral Dissertation, Johannes Gutenberg Universität Mainz. Accessed December 10, 2019.
http://ubm.opus.hbz-nrw.de/volltexte/2012/3241/.
MLA Handbook (7th Edition):
Vasel, Matthäus. “Untersuchungen zu der Rolle der extrazellulären Matrix bei Fibrose und bei Tumoren.” 2012. Web. 10 Dec 2019.
Vancouver:
Vasel M. Untersuchungen zu der Rolle der extrazellulären Matrix bei Fibrose und bei Tumoren. [Internet] [Doctoral dissertation]. Johannes Gutenberg Universität Mainz; 2012. [cited 2019 Dec 10].
Available from: http://ubm.opus.hbz-nrw.de/volltexte/2012/3241/.
Council of Science Editors:
Vasel M. Untersuchungen zu der Rolle der extrazellulären Matrix bei Fibrose und bei Tumoren. [Doctoral Dissertation]. Johannes Gutenberg Universität Mainz; 2012. Available from: http://ubm.opus.hbz-nrw.de/volltexte/2012/3241/
University of Alberta
9.
Onwuchekwa, Nnenna E.
Enhanced revegetation and reclamation of oil sand disturbed
land using mycorrhizae.
Degree: MS, Department of Renewable Resources, 2012, University of Alberta
URL: https://era.library.ualberta.ca/files/cv43nz239
► This study examined the potential of using conifer seedlings Picea glauca and Pinus banksiana inoculated with ectomycorrhizal (ECM) fungi to improve revegetation success and plant…
(more)
▼ This study examined the potential of using conifer
seedlings Picea glauca and Pinus banksiana inoculated with
ectomycorrhizal (ECM) fungi to improve revegetation success and
plant establishment in reclaimed oil sands mining sites.
Mycorrhizal inoculum potential of the reclamation soils was low
with the maximum inoculum potential of 23% and 29%for ECM and
arbuscular mycorrhizae, respectively. The response of seedlings in
the field to ECM inoculation varied between plant species and
measured parameters. A significant effect of ECM inoculation on
height was observed in P. banksiana but not in P. glauca. The
average survival rate for P. glauca seedlings inoculated with
different species of ECM varied between 36% and 56%, whereas the
control (uninoculated) seedlings had minimum and maximum survival
rates of 22 and 41% respectively. Generally, it was construed that
the re-introduction of mycorrhizal fungi during reclamation process
is an important approach that should be further
exploited.
Subjects/Keywords: Mycorrhizae; ECM fungi; Revegetation; Oil sands; Reclamation
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APA (6th Edition):
Onwuchekwa, N. E. (2012). Enhanced revegetation and reclamation of oil sand disturbed
land using mycorrhizae. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/cv43nz239
Chicago Manual of Style (16th Edition):
Onwuchekwa, Nnenna E. “Enhanced revegetation and reclamation of oil sand disturbed
land using mycorrhizae.” 2012. Masters Thesis, University of Alberta. Accessed December 10, 2019.
https://era.library.ualberta.ca/files/cv43nz239.
MLA Handbook (7th Edition):
Onwuchekwa, Nnenna E. “Enhanced revegetation and reclamation of oil sand disturbed
land using mycorrhizae.” 2012. Web. 10 Dec 2019.
Vancouver:
Onwuchekwa NE. Enhanced revegetation and reclamation of oil sand disturbed
land using mycorrhizae. [Internet] [Masters thesis]. University of Alberta; 2012. [cited 2019 Dec 10].
Available from: https://era.library.ualberta.ca/files/cv43nz239.
Council of Science Editors:
Onwuchekwa NE. Enhanced revegetation and reclamation of oil sand disturbed
land using mycorrhizae. [Masters Thesis]. University of Alberta; 2012. Available from: https://era.library.ualberta.ca/files/cv43nz239
Texas A&M University
10.
Cramlet, Andrew Charles.
Performance of ECM Controlled VAV Fan Powered Terminal Units.
Degree: 2009, Texas A&M University
URL: http://hdl.handle.net/1969.1/ETD-TAMU-3000
► Empirical performance models of fan airflow, primary airflow and power consumption were developed for series and parallel variable air volume fan powered terminal units. An…
(more)
▼ Empirical performance models of fan airflow, primary airflow and power consumption were
developed for series and parallel variable air volume fan powered terminal units. An
experimental setup and test procedure were created to test the terminal units at typical design
pressures and airflows. Each terminal unit observed in this study used an 8 in (20.3 cm) primary
air inlet. Two fan motor control methods were considered. The primary control of interest was
the electronically commutated motor (
ECM) controller. Data collected were compared with
previous research regarding silicon rectified control (SCR) units. Generalized models were
developed for both series and parallel terminal units. Coefficients for performance models were
then compared with comparable SCR controlled units. Non-linear statistical modeling was
performed using SPSS software (2008).
In addition to airflow and power consumption modeling, power quality was also quantified.
Relationships between real power (watts) and apparent power (VA) were presented as well as
harmonic frequencies and total harmonic distortion. Power quality was recorded for each
ECM
controlled terminal unit tested. Additional tests were also made to SCR controlled terminal units
used in previous research (Furr 2006).
The airflow and power consumption performance models had an R2 equal to 0.990 or greater for
every terminal unit tested. An air leakage model was employed to account for leakage in the
parallel designed VAV terminal units when the internal fan was turned off. For the leakage
model, both
ECM and SCR controlled units achieved an R2 greater than or equal to 0.918.
Advisors/Committee Members: O'Neal, Dennis (advisor), Heffington, Warren (committee member), Jackson, Jerry (committee member).
Subjects/Keywords: ECM; VAV; FPTU; Fan; Powered; Terminal; Unit
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Manager
APA (6th Edition):
Cramlet, A. C. (2009). Performance of ECM Controlled VAV Fan Powered Terminal Units. (Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-3000
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Cramlet, Andrew Charles. “Performance of ECM Controlled VAV Fan Powered Terminal Units.” 2009. Thesis, Texas A&M University. Accessed December 10, 2019.
http://hdl.handle.net/1969.1/ETD-TAMU-3000.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Cramlet, Andrew Charles. “Performance of ECM Controlled VAV Fan Powered Terminal Units.” 2009. Web. 10 Dec 2019.
Vancouver:
Cramlet AC. Performance of ECM Controlled VAV Fan Powered Terminal Units. [Internet] [Thesis]. Texas A&M University; 2009. [cited 2019 Dec 10].
Available from: http://hdl.handle.net/1969.1/ETD-TAMU-3000.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Cramlet AC. Performance of ECM Controlled VAV Fan Powered Terminal Units. [Thesis]. Texas A&M University; 2009. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-3000
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
11.
Wu, Jia-ling.
Nonlinear Analysis in Investment and Trade across Taiwan Starit.
Degree: Master, Economics, 2008, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0716108-143710
► In this paper, we examine the relationship between outward FDI and trades across Taiwan Strait during the period 1994Q4 to 2007Q4. We modify the export…
(more)
▼ In this paper, we examine the relationship between outward FDI and trades across Taiwan Strait during the period 1994Q4 to 2007Q4. We modify the export function, import function and trade function by including outward FDI variable to reflect the effect of bilateral trade between Taiwan and China. Empirical evidences indicate that the coefficient is significantly crucial to the long-run relationship. Due to the failure of a linear error-correction model (
ECM) in describing the dynamics of exports and trades, we apply a nonlinear
ECM to examine its dynamics and find supporting to the appropriateness of the nonlinear model empirically. Furthermore, outward FDI in percentages of Taiwan GDP was found increasing exports and decreasing imports across Taiwan Strait in percentages of Taiwan GDP. Then, the results also indicate the possibility of a positive effect of the ratio of China GDP to Taiwan GDP on the ratio of import to Taiwan GDP and a negative effect of the ratio of export to Taiwan GDP. It possibly offsets the balance of trade surplus at the same time. Finally, the bilateral trade across Taiwan Strait raises while the FDI ratio is increasing. China becomes the most important trade partner to Taiwan in recent years.
Advisors/Committee Members: Yung-hsiang Ying (chair), ching-nun Lee (chair), Ming-Jang Weng (committee member).
Subjects/Keywords: trade; China investment; STAR; nonlinear ECM
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Manager
APA (6th Edition):
Wu, J. (2008). Nonlinear Analysis in Investment and Trade across Taiwan Starit. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0716108-143710
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Wu, Jia-ling. “Nonlinear Analysis in Investment and Trade across Taiwan Starit.” 2008. Thesis, NSYSU. Accessed December 10, 2019.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0716108-143710.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Wu, Jia-ling. “Nonlinear Analysis in Investment and Trade across Taiwan Starit.” 2008. Web. 10 Dec 2019.
Vancouver:
Wu J. Nonlinear Analysis in Investment and Trade across Taiwan Starit. [Internet] [Thesis]. NSYSU; 2008. [cited 2019 Dec 10].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0716108-143710.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Wu J. Nonlinear Analysis in Investment and Trade across Taiwan Starit. [Thesis]. NSYSU; 2008. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0716108-143710
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
12.
Randles, Michael.
Proteomic analyses of kidney glomerular extracellular
matrix in health and disease.
Degree: 2015, University of Manchester
URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:252839
► Glomerular filtration is a vital physiological process removing waste products from the circulation and this process occurs across the glomerular filtration barrier (GFB). The cells…
(more)
▼ Glomerular filtration is a vital physiological
process removing waste products from the circulation and this
process occurs across the glomerular filtration barrier (GFB). The
cells and extracellular matrix (ECM), which form this barrier, are
exposed to forces during ultrafiltration and special adaptation is
required to withstand these forces. Dysfunction in cellular
adhesion machinery or ECM assembly within the GFB causes loss of
selective glomerular filtration, however, the mechanisms governing
these processes are poorly understood. To this end we sought to
characterise the glomerular ECM and adhesion machinery using high
throughput mass spectrometry (MS)-based proteomics.MS of human
glomerular ECM identified a highly complex extracellular niche,
revealing the potential involvement of novel ECM proteins in
glomerular development and disease processes. Furthermore we
identified that glomerular cells in culture had distinct ECM
proteomes and interestingly, coculture experiments demonstrated
that the ECM proteome was influenced by cellular crosstalk and had
a closer resemblance to glomerular ECM in vivo. Protein network
analyses of in vivo and in vitro ECM datasets revealed a common
core of highly connected structural ECM proteins that may be
important for glomerular ECM assembly. To understand how this ECM
proteome altered in disease, we studied mice with mild glomerular
dysfunction. Here, transcriptomic and proteomic analyses identified
alterations in ECM composition and 3D electron microscopy revealed
striking ultrastructural changes in glomerular ECM. MS-based
proteomics was next applied to the analysis of glomerular podocyte
adhesion complexes, leading to the discovery that the actin
cytoskeletal regulators and trafficking machinery are recruited to
adhesions sites in an ECM-ligand dependent manner. Furthermore,
these differences functionally altered cell shape and adhesion
strength. These same analyses were applied to podocyte cell-cell
junctions, revealing an unexpected overlap of cell-ECM and
cell-cell adhesion machinery.Overall, these findings demonstrate
for the first time the complexity of the glomerular ECM and
adhesion signalling complexes and reinforce the benefits of global,
unbiased experimental approaches. In addition the results suggest
that glomerular ECM composition, organisation and adhesion
signalling are context dependent, and therefore, represent
potential therapeutic targets.
Movies of 3view of glomerular basement membranes,
movies of live cell imaging podocytes on collagen IV and on
laminin
none
Advisors/Committee Members: WOOLF, ADRIAN AS, Lennon, Rachel, Woolf, Adrian.
Subjects/Keywords: ECM; Proteomics
…138
Figure 3.12 The effects of coculture on ECM deposition and cell-cell junctions… …139
Figure 3.13 Coculture ECM interaction network analysis… …147
Figure 4.2 Genetic background is a regulator of ECM composition… …149
Figure 4.3 Topological network analysis of glomerular ECM protein interactions… …151
Figure 4.4 Network analysis of enriched glomerular ECM proteins…
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APA ·
Chicago ·
MLA ·
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Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Randles, M. (2015). Proteomic analyses of kidney glomerular extracellular
matrix in health and disease. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:252839
Chicago Manual of Style (16th Edition):
Randles, Michael. “Proteomic analyses of kidney glomerular extracellular
matrix in health and disease.” 2015. Doctoral Dissertation, University of Manchester. Accessed December 10, 2019.
http://www.manchester.ac.uk/escholar/uk-ac-man-scw:252839.
MLA Handbook (7th Edition):
Randles, Michael. “Proteomic analyses of kidney glomerular extracellular
matrix in health and disease.” 2015. Web. 10 Dec 2019.
Vancouver:
Randles M. Proteomic analyses of kidney glomerular extracellular
matrix in health and disease. [Internet] [Doctoral dissertation]. University of Manchester; 2015. [cited 2019 Dec 10].
Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:252839.
Council of Science Editors:
Randles M. Proteomic analyses of kidney glomerular extracellular
matrix in health and disease. [Doctoral Dissertation]. University of Manchester; 2015. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:252839
Queensland University of Technology
13.
Tan, Yu Pei.
The development of Lactococcus lactis as an antimicrobial agent.
Degree: 2010, Queensland University of Technology
URL: https://eprints.qut.edu.au/39143/
► Non-pathogenic lactic acid bacteria are economically important Gram-positive bacteria used extensively in the food industry. Due to their “generally regarded as safe” status, certain species…
(more)
▼ Non-pathogenic lactic acid bacteria are economically important Gram-positive bacteria used extensively in the food industry. Due to their “generally regarded as safe” status, certain species from the genera Lactobacillus and Lactococcus are also considered desirable as candidates for the production and secretion of recombinant proteins, particular those with therapeutic applications.
The hypothesis examined by this thesis is that Lactococcus lactis can be modified to be an effective antimicrobial agent. Therefore, the aims of this thesis were to investigate the optimisation of the expression, secretion and/or activities of potential heterologous antimicrobial proteins by the model lactic acid bacterium, Lactococcus lactis subsp. cremoris MG1363.
L. lactis strains were engineered to express and secrete the recombinant CyuC surface protein from Lactobacillus reuteri BR11, and a fusion protein consisting of CyuC and lysostaphin using the Sep promoter and secretion signal. CyuC has been characterised as a cystine-binding protein, but has also been demonstrated to have fibronectin binding activity. Lysostaphin is a bacteriolytic enzyme with specific activity against the Gram-positive pathogen, Staphylococcus aureus. These modified L. lactis strains were then investigated to see if they had the ability to inhibit the adhesion of S. aureus to host extracellular matrix (ECM) proteins. It was observed that the cell extracts of the L. lactis strain with the vector only (pGhost9:ISS1) was able to inhibit the adhesion of S. aureus to fibronectin, whilst the cell extracts of the L. lactis strain expressing lysostaphin was able to inhibit adhesion to keratin.
Finally, this thesis has identified specific lactococcal genes that affect the secretion of lysostaphin through the use of random transposon mutagenesis. Ten mutants with higher lysostaphin activity contained insertions in four different genes encoding: (i) an uncharacterised putative transmembrane protein (llmg_0609), (ii) an enzyme catalysing the first step in peptidoglycan biosynthesis (murA2), (iii) a homolog of the oxidative defence regulator (trmA), and (iv) an uncharacterised putative enzyme involved in ubiquinone biosynthesis (llmg_2148). The higher lysostaphin activity observed in these mutants was found to be due to higher amounts of lysostaphin being secreted.
The findings of this thesis contribute to the development of this organism as an antimicrobial agent and also to our understanding of L. lactis genetics.
Subjects/Keywords: Lactococcus; recombinant protein secretion; lysostaphin; ECM proteins
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to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Tan, Y. P. (2010). The development of Lactococcus lactis as an antimicrobial agent. (Thesis). Queensland University of Technology. Retrieved from https://eprints.qut.edu.au/39143/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Tan, Yu Pei. “The development of Lactococcus lactis as an antimicrobial agent.” 2010. Thesis, Queensland University of Technology. Accessed December 10, 2019.
https://eprints.qut.edu.au/39143/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Tan, Yu Pei. “The development of Lactococcus lactis as an antimicrobial agent.” 2010. Web. 10 Dec 2019.
Vancouver:
Tan YP. The development of Lactococcus lactis as an antimicrobial agent. [Internet] [Thesis]. Queensland University of Technology; 2010. [cited 2019 Dec 10].
Available from: https://eprints.qut.edu.au/39143/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Tan YP. The development of Lactococcus lactis as an antimicrobial agent. [Thesis]. Queensland University of Technology; 2010. Available from: https://eprints.qut.edu.au/39143/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of California – Irvine
14.
Hsieh, Jessica Yi-Chia.
Mechanochemical Regulation of Macrophage Inflammatory Activation by Fibrin(ogen).
Degree: Biomedical Engineering, 2018, University of California – Irvine
URL: http://www.escholarship.org/uc/item/2cw39187
► Proper wound healing still remains a clinical challenge. Fibrin is a major component of the provisional extracellular matrix that forms initially after injury, enabling cell…
(more)
▼ Proper wound healing still remains a clinical challenge. Fibrin is a major component of the provisional extracellular matrix that forms initially after injury, enabling cell infiltration and anchoring to the wound site. Fibrinogen, the precursor of fibrin and a blood plasma protein, is also important in inflammation, wound healing, angiogenesis, and other biological functions. Of the many cells present during the wound healing process, macrophages play a key role in modulating the immune response. The plasticity of macrophages permits them to adopt behaviors along a spectrum of phenotypes depending on signals in their microenvironment. While much is known about how chemokines, cytokines, and other soluble mediators influence macrophages, less is understood about the roles of the ECM and the physical properties of the surrounding tissue. In this study, we examined the interactions between fibrin(ogen) and macrophages to gain a better fundamental understanding of how physical and biochemical properties of the provisional matrix together influence immune cell behavior. We separately looked at the roles fibrinogen and fibrin on macrophage inflammatory or anti-inflammatory functions and explored how mechanical properties of fibrin(ogen) such as substrate stiffness and tethering affect macrophage activation and behavior.First, we investigated the differential regulation of macrophage activation by fibrinogen and fibrin. The presentation of fibrin(ogen), either presented in the soluble form or incorporated within an insoluble matrix, plays an important role in regulating macrophage phenotype. In particular, fibrin exerts a protective effect on macrophage inflammatory activation. Additionally, we altered mechanical properties of fibrin by controlling matrix stiffness and explored methods of adsorbing or tethering fibrinogen. Substrate rigidity alters the expression of integrins, proteins receptors that facilitate macrophage-ECM interactions. Adsorption of fibrinogen also inhibits inflammatory activation. Finally, we utilized a light-based crosslinking technique to stiffen fibrin matrices. Increased substrate rigidity enhances inflammatory macrophage behavior. Moreover, macrophages are more motile, both in terms of velocity and distance traveled, on stiffer fibrin matrices. Together, these findings contribute to our understanding of how the extracellular matrix regulates macrophages during wound healing and disease and offer new insight for designing biomaterials that can precisely control the host response and promote proper tissue regeneration.
Subjects/Keywords: Bioengineering; ECM; Fibrin; Macrophage; Mechanobiology; Migration; Stiffness
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APA ·
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Manager
APA (6th Edition):
Hsieh, J. Y. (2018). Mechanochemical Regulation of Macrophage Inflammatory Activation by Fibrin(ogen). (Thesis). University of California – Irvine. Retrieved from http://www.escholarship.org/uc/item/2cw39187
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Hsieh, Jessica Yi-Chia. “Mechanochemical Regulation of Macrophage Inflammatory Activation by Fibrin(ogen).” 2018. Thesis, University of California – Irvine. Accessed December 10, 2019.
http://www.escholarship.org/uc/item/2cw39187.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Hsieh, Jessica Yi-Chia. “Mechanochemical Regulation of Macrophage Inflammatory Activation by Fibrin(ogen).” 2018. Web. 10 Dec 2019.
Vancouver:
Hsieh JY. Mechanochemical Regulation of Macrophage Inflammatory Activation by Fibrin(ogen). [Internet] [Thesis]. University of California – Irvine; 2018. [cited 2019 Dec 10].
Available from: http://www.escholarship.org/uc/item/2cw39187.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Hsieh JY. Mechanochemical Regulation of Macrophage Inflammatory Activation by Fibrin(ogen). [Thesis]. University of California – Irvine; 2018. Available from: http://www.escholarship.org/uc/item/2cw39187
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
15.
Stenberg, Richard.
Utrustning för automatisk matning av ändkapningsmaskin för lysrör.
Degree: 2004, , Department of Mechanical Engineering
URL: http://urn.kb.se/resolve?urn=urn:nbn:se:bth-2874
► Målet med arbetet har varit att utveckla utrustning för automatisk inmatning av lysrör till MRT:s ECM (End Cut Machine). ECM är en maskin som…
(more)
▼ Målet med arbetet har varit att utveckla utrustning för automatisk inmatning av lysrör till MRT:s ECM (End Cut Machine). ECM är en maskin som används för att kapa av ändarna på lysrör genom termisk chock och återvinna det kvicksilverhaltiga lyspulver som lysrören innehåller. Arbetet inleddes med att en produktdefinition sattes upp där aspekter såsom hur processen i stort går till, vilken omgivning utrustningen skall verka i , vilka människor som berörs av utrustningen och ekonomiska frågor behandlas. Efter produktdefinitionen följer en produktundersökning. Det föreligger ett starkt önskemål från MRT:s kunder att ECM ska levereras med möjlighet till automatisk inmatning av lysrör. I dagsläget finns inte finns några tidigare lösningar på problemet som uppfyller kraven tillfredställande. Nästa steg var att göra en kravuppställning. Där listas krav inom kategorierna funktion, ekonomi, miljö, ergonomi och säkerhet. Alla krav har inte kunnat implementeras eftersom projektet inte har lett till en slutgiltig produkt utan avslutades med byggandet och testandet av en prototyp. För att bestämma hur utrustningen skulle se ut togs ett antal produktförslag fram. Dessa jämfördes inbördes och i förhållande till tidigare erfarenheter av lysrörs egenskaper. Förslaget som ansågs ha störst potential att uppfylla kraven vidareutvecklades ytterligare och ritningar ritades upp i Autocad. Det vidareutvecklade förslaget har relativt låg teknisk komplexitet och utvecklingskostnaderna kan därför hållas låga. Förslaget bygger på att lysrören hela tiden hålls i rörelse med hjälp av transportband för att undvika stopp i flödet. För att se om förslaget fungerade som tänkt konstruerades en prototyp. Test av prototypen påvisade att ett antal modifikationer var nödvändiga. När modifikationerna var genomförda löste prototypen uppgiften att från ett magasin med en stor mängd lysrör leverera ett jämnt flöde. Prototypen är dock inte byggd i full skala och därmed inte testad med så stora volymer som den slutgiltiga produkten är tänkt att klara.
Subjects/Keywords: Lysrör; ECM; ändkapning
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APA (6th Edition):
Stenberg, R. (2004). Utrustning för automatisk matning av ändkapningsmaskin för lysrör. (Thesis). , Department of Mechanical Engineering. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:bth-2874
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Stenberg, Richard. “Utrustning för automatisk matning av ändkapningsmaskin för lysrör.” 2004. Thesis, , Department of Mechanical Engineering. Accessed December 10, 2019.
http://urn.kb.se/resolve?urn=urn:nbn:se:bth-2874.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Stenberg, Richard. “Utrustning för automatisk matning av ändkapningsmaskin för lysrör.” 2004. Web. 10 Dec 2019.
Vancouver:
Stenberg R. Utrustning för automatisk matning av ändkapningsmaskin för lysrör. [Internet] [Thesis]. , Department of Mechanical Engineering; 2004. [cited 2019 Dec 10].
Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:bth-2874.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Stenberg R. Utrustning för automatisk matning av ändkapningsmaskin för lysrör. [Thesis]. , Department of Mechanical Engineering; 2004. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:bth-2874
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Ottawa
16.
Yuan, Yifan.
Enhancing Blood Outgrowth Endothelial Cells for Optimal Coating of Blood Contacting Surfaces
.
Degree: 2017, University of Ottawa
URL: http://hdl.handle.net/10393/36837
► Implantable cardiovascular biomaterials have been widely applied in multiple cardiovascular disorders such as coronary artery disease, heart failure, and abdominal aortic aneurysms. However the failure…
(more)
▼ Implantable cardiovascular biomaterials have been widely applied in multiple cardiovascular disorders such as coronary artery disease, heart failure, and abdominal aortic aneurysms. However the failure modes of cardiovascular biomaterials are not uncommon, which is mainly due to the complications on blood-contacting surfaces such as thrombosis, calcification, and inflammation. Endothelium locates the inner surface of vessel lumen and is a critical regulator of vascular homeostasis. However, a readily available functional autologous source of endothelium has been hard to achieve. Human blood outgrowth endothelial cells (BOECs), cultured from peripheral blood mononuclear cells are proliferative and express endothelial protein profiles and as such are a very promising novel cell source for cardiovascular biomaterials coating. Endothelial nitric oxide synthase (eNOS) is an important regulator of vascular homeostasis and loss of eNOS activity is a hallmark of endothelial dysfunction. My data demonstrated that BOECs express markedly lower eNOS protein, mRNA as well as activity levels when compared to mature endothelial cells (ECs). My first project was to use transient transfection methods along with minicircle DNA to enhance eNOS expression levels in BOECs. Two promoters were tested in BOECs, the CMV promoter (pMini-CMV-eNOS) and the EF1α promoter (pMini-EF1α-eNOS). Transfection with pMini-CMV-eNOS achieved 24.8 ± 5.1 times more eNOS expression when compared to null transfected cells at 24 hours, a marked improvement over that achieved with conventional PVAX plasmid (10.2 ± 4.7 fold increase) or pMini-EF1α-eNOS (8.2 ± 1.2 fold increase both compared to null transfected control). pMini-CMV-eNOS mediated overexpression improved cell migration and network formation. When cultured on Osteopontin (OPN) coated surfaces, transient transfection with plasmid eNOS in BOECs can markedly enhance cell spreading and adhesion to ECM modified surfaces. These results suggest that eNOS expression in BOECs is suboptimal and BOECs may be functionally improved by techniques to enhance expression of this critical homeostatic regulator.
Extracellular matrix (ECM) proteins have been shown to negatively regulate eNOS expression and NO production in mature ECs. In addition, the deposition of Col IV and Col I in BOECs is higher compared to that in mature ECs. Thus, I have proposed that the lower eNOS expression/activity in BOECs compared to mature ECs is due to higher ECM deposition. When grown on fibronectin, type I collagen, type IV collagen and laminin, significantly decreased eNOS protein in HUVECs were found compared to cells on polystyrene. Interestingly, when cultured on polystyrene, BOECs express significantly more extracellular matrix (ECM) proteins especially type I collagen compared to mature ECs. Blocking collagen synthesis significantly enhanced eNOS expression in BOECs (1.77 ± 0.41 fold increase). My results suggest that the regulation of eNOS in BOECs and mature ECs is similar and the reduced eNOS level in BOECs may be due to their…
Subjects/Keywords: BOECs;
ECM;
eNOS;
Blood contacting surface
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APA (6th Edition):
Yuan, Y. (2017). Enhancing Blood Outgrowth Endothelial Cells for Optimal Coating of Blood Contacting Surfaces
. (Thesis). University of Ottawa. Retrieved from http://hdl.handle.net/10393/36837
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Yuan, Yifan. “Enhancing Blood Outgrowth Endothelial Cells for Optimal Coating of Blood Contacting Surfaces
.” 2017. Thesis, University of Ottawa. Accessed December 10, 2019.
http://hdl.handle.net/10393/36837.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Yuan, Yifan. “Enhancing Blood Outgrowth Endothelial Cells for Optimal Coating of Blood Contacting Surfaces
.” 2017. Web. 10 Dec 2019.
Vancouver:
Yuan Y. Enhancing Blood Outgrowth Endothelial Cells for Optimal Coating of Blood Contacting Surfaces
. [Internet] [Thesis]. University of Ottawa; 2017. [cited 2019 Dec 10].
Available from: http://hdl.handle.net/10393/36837.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Yuan Y. Enhancing Blood Outgrowth Endothelial Cells for Optimal Coating of Blood Contacting Surfaces
. [Thesis]. University of Ottawa; 2017. Available from: http://hdl.handle.net/10393/36837
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Toronto
17.
Baratta, Cristina.
SPARC is Required in the Drosophila melanogaster Fat Body for ECM Homeostasis during Larval Development.
Degree: 2012, University of Toronto
URL: http://hdl.handle.net/1807/33330
► SPARC is a collagen‐binding, matricellular glycoprotein with diverse roles in tissue remodeling and development. Previous studies have demonstrated that SPARC is required in Drosophila for…
(more)
▼ SPARC is a collagen‐binding, matricellular glycoprotein with diverse roles in tissue remodeling and development. Previous studies have demonstrated that SPARC is required in Drosophila for larval development and maintenance of the fat body, an organ that incorporates endocrine, growth and immune functions. I have characterized effects of loss and knockdown of SPARC in the fat body. Loss‐of‐function analyses revealed remodeling of adipocytes demarcated by cell rounding and dense accumulation of extracellular matrix (ECM) beneath an abnormally thick basement membrane. Remodeling of adipocytes
mediated by expression of matrix metalloproteinase 2 (MMP2) was found to cause ECM breakdown and accumulation of hemocytes, indicating endogenous fat body remodeling is mechanistically distinct from that which occurs upon silencing of SPARC. Knockdown of the lysyl hydroxylase, dPlod, in the fat body, revealed abnormal intracellular co‐localization of SPARC with Collagen IV, but not with Laminin. The data indicate SPARC is required for ECM homeostasis during development.
MAST
Advisors/Committee Members: Ringuette, Maurice, Cell and Systems Biology.
Subjects/Keywords: SPARC; Drosophila; Fat Body; ECM; 0379
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APA (6th Edition):
Baratta, C. (2012). SPARC is Required in the Drosophila melanogaster Fat Body for ECM Homeostasis during Larval Development. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/33330
Chicago Manual of Style (16th Edition):
Baratta, Cristina. “SPARC is Required in the Drosophila melanogaster Fat Body for ECM Homeostasis during Larval Development.” 2012. Masters Thesis, University of Toronto. Accessed December 10, 2019.
http://hdl.handle.net/1807/33330.
MLA Handbook (7th Edition):
Baratta, Cristina. “SPARC is Required in the Drosophila melanogaster Fat Body for ECM Homeostasis during Larval Development.” 2012. Web. 10 Dec 2019.
Vancouver:
Baratta C. SPARC is Required in the Drosophila melanogaster Fat Body for ECM Homeostasis during Larval Development. [Internet] [Masters thesis]. University of Toronto; 2012. [cited 2019 Dec 10].
Available from: http://hdl.handle.net/1807/33330.
Council of Science Editors:
Baratta C. SPARC is Required in the Drosophila melanogaster Fat Body for ECM Homeostasis during Larval Development. [Masters Thesis]. University of Toronto; 2012. Available from: http://hdl.handle.net/1807/33330
Tampere University
18.
Aholainen, Emmi.
Sisällönhallinta suomalaisessa julkishallinnossa
.
Degree: 2017, Tampere University
URL: https://trepo.tuni.fi/handle/10024/100882
► Tutkimuksessa tarkasteltiin sisällönhallintaa ja enterprise content managementia (ECM) suomalaisessa julkishallinnossa. Tutkimuksen ensisijaisina tavoitteina oli kartoittaa, missä määrin kohdeorganisaatioissa on käytössä menetelmiä sähköisten informaatiosisältöjen hallintaan sekä…
(more)
▼ Tutkimuksessa tarkasteltiin sisällönhallintaa ja enterprise content managementia (ECM) suomalaisessa julkishallinnossa. Tutkimuksen ensisijaisina tavoitteina oli kartoittaa, missä määrin kohdeorganisaatioissa on käytössä menetelmiä sähköisten informaatiosisältöjen hallintaan sekä millaisia haasteita ja ongelma-alueita organisaatioiden sisällön- ja tiedonhallinnassa ilmenee.
Tutkimus toteutettiin verkkokyselynä, joka välitettiin sähköpostitse 25:lle satunnaisotannalla valitulle organisaatiolle julkishallinnon eri toimialoilta. Tutkimus oli lähtökohdiltaan laadullinen, ja aineiston analyysissä sovellettiin kohdeorganisaatioiden luokittelua eri kategorioihin niissä hyödynnettyjen sisällönhallinnan menetelmien perusteella.
Tutkimustuloksista ilmeni, että suomalaisessa julkishallinnossa toimitaan toistaiseksi tilanteessa, jossa paperiasiakirjojen käsittelystä ja säilytyksestä ollaan siirtymässä kohti sähköistä toimintaympäristöä. Samanaikaisesti myös muiden sähköisten informaatiosisältöjen hallintaan on alettu kiinnittämään alustavaa huomiota, mutta koko organisaation kattavan ECM-ratkaisun hyödyntäminen on vielä vähäistä.
Erityishaasteita organisaatioille tuottivat etenkin tiedonhallinnan järjestelmien välisen integraation puutteet ja järjestelmien käytettävyysongelmat. Kyseiset haasteet puolestaan heikensivät työntekijöiden sitoutumista yhteisten järjestelmien käyttöön. Lisäksi ongelmaksi koettiin informaation löydettävyys, mikä johtui muun muassa asiakirjojen ja muun informaation moninaisuudesta, hakutoimintojen puutteista sekä saman informaation säilytyksestä päällekkäisissä tallennuspaikoissa.
Tulosten perusteella on pääteltävissä, että asiakirjahallinnon sähköistäminen on julkisella sektorilla sisällönhallinnan muita osa-alueita ajankohtaisempi tavoite. Julkishallinnon tiedonhallintaa voitaisi kuitenkin tehostaa hyödyntämällä laajemmin tiettyjä ECM:in piiriin kuuluvia menetelmiä ja liiketoimintajärjestelmiä. Muun muassa kokonaisarkkitehtuuri, genreanalyysi, organisaation kattavat hakusovellukset ja sähköpostien hallinta- ja arkistointijärjestelmä voisivat tarjota ratkaisuja tiedonhallinnassa kohdattuihin haasteisiin.
Subjects/Keywords: sisällönhallinta;
ECM;
tiedonhallinta;
asiakirjahallinto;
julkinen hallinto
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APA ·
Chicago ·
MLA ·
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Export
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Manager
APA (6th Edition):
Aholainen, E. (2017). Sisällönhallinta suomalaisessa julkishallinnossa
. (Masters Thesis). Tampere University. Retrieved from https://trepo.tuni.fi/handle/10024/100882
Chicago Manual of Style (16th Edition):
Aholainen, Emmi. “Sisällönhallinta suomalaisessa julkishallinnossa
.” 2017. Masters Thesis, Tampere University. Accessed December 10, 2019.
https://trepo.tuni.fi/handle/10024/100882.
MLA Handbook (7th Edition):
Aholainen, Emmi. “Sisällönhallinta suomalaisessa julkishallinnossa
.” 2017. Web. 10 Dec 2019.
Vancouver:
Aholainen E. Sisällönhallinta suomalaisessa julkishallinnossa
. [Internet] [Masters thesis]. Tampere University; 2017. [cited 2019 Dec 10].
Available from: https://trepo.tuni.fi/handle/10024/100882.
Council of Science Editors:
Aholainen E. Sisällönhallinta suomalaisessa julkishallinnossa
. [Masters Thesis]. Tampere University; 2017. Available from: https://trepo.tuni.fi/handle/10024/100882
Princeton University
19.
Han, Siyang.
BIOMECHANICAL REGULATION OF CELL PROLIFERATION, EPITHELIAL-MESENCHYMAL TRANSITION AND INVASION
.
Degree: PhD, 2019, Princeton University
URL: http://arks.princeton.edu/ark:/88435/dsp01pk02cd52s
► Cells in epithelial tissues adhere to an underlying extracellular matrix (ECM) and respond to changes in its physical properties by altering gene expression and behavior.…
(more)
▼ Cells in epithelial tissues adhere to an underlying extracellular matrix (
ECM) and respond to changes in its physical properties by altering gene expression and behavior. The mechanical properties of the
ECM, including the matrix stiffness, are therefore central to developmental, physiological, and pathological processes. In the context of cancer, increased tissue stiffness and interstitial fluid pressure are inherent features of tumorigenesis, which promote malignant transformation of surrounding cells and correlate with poor survival. These processes depend on the synergistic effects of mechanical signals such as
ECM stiffness and chemical signals such as Wnt, TGFβ and proteases in the cellular microenvironment. This dissertation explores how mechanical and biochemical signals are integrated to control cell behaviors including proliferation, apoptosis, epithelial-mesenchymal transition (EMT), and invasion. We used engineered substrata to recapitulate the mechanical stiffnesses of the normal mammary gland as well as that of breast tumors.
We found that exposure to Wnt3a increased proliferation of mammary epithelial cells cultured on stiff substrata, with compliances characteristic of breast tumors, but not of cells on soft substrata, with compliances comparable to that of normal mammary tissue. Depleting integrin-linked kinase (ILK), which functions as a signaling hub for cellular mechanotransduction, rendered cells unresponsive to Wnt3a on both substrata. Ectopic expression of ILK permitted Wnt3a to induce proliferation of cells on both microenvironments. We further showed that ILK regulates expression of the Wnt receptor frizzled-1 (Fzd1), suggesting the existence of a positive feedback loop between Wnt3a, ILK and Fzd1.
Following these findings, we examined the role of ILK and tissue stiffness in regulating cell fate in response to TGFβ1. We found that ILK controls the switch between apoptosis and EMT downstream of TGFβ1 through modulating the fine balance between cell-cell and cell-matrix adhesions. Specifically, depletion of ILK leads to increased E-cadherin-based cell-cell adhesion and cortical actin and decreased focal adhesions and stress fibers in mammary epithelial cells. These changes favor apoptosis and suppress EMT downstream of TGFβ1, regardless of matrix stiffness. Similarly, soft matrix also favors apoptosis downstream of TGFβ1 while stiff matrix promotes EMT, suggesting that depletion of ILK disrupts the ability of a cell to sense a mechanically stiff microenvironment.
Taken together, these findings suggest that tissue mechanics regulates the cellular response to Wnt and TGFβ under physiological and pathological microenvironmental conditions. We also highlighted the role of ILK in mediating the responses to matrix stiffness and tuning the proliferative and potential tumorigenic behaviors of mammary epithelial cells to Wnt3a and TGFβ1. These results provide insight into the molecular mechanisms underlying the effects of a pathological microenvironment on neoplastic…
Advisors/Committee Members: Nelson, Celeste M (advisor).
Subjects/Keywords: apoptosis;
ECM;
EMT;
ILK;
proliferation;
Wnt3a
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APA (6th Edition):
Han, S. (2019). BIOMECHANICAL REGULATION OF CELL PROLIFERATION, EPITHELIAL-MESENCHYMAL TRANSITION AND INVASION
. (Doctoral Dissertation). Princeton University. Retrieved from http://arks.princeton.edu/ark:/88435/dsp01pk02cd52s
Chicago Manual of Style (16th Edition):
Han, Siyang. “BIOMECHANICAL REGULATION OF CELL PROLIFERATION, EPITHELIAL-MESENCHYMAL TRANSITION AND INVASION
.” 2019. Doctoral Dissertation, Princeton University. Accessed December 10, 2019.
http://arks.princeton.edu/ark:/88435/dsp01pk02cd52s.
MLA Handbook (7th Edition):
Han, Siyang. “BIOMECHANICAL REGULATION OF CELL PROLIFERATION, EPITHELIAL-MESENCHYMAL TRANSITION AND INVASION
.” 2019. Web. 10 Dec 2019.
Vancouver:
Han S. BIOMECHANICAL REGULATION OF CELL PROLIFERATION, EPITHELIAL-MESENCHYMAL TRANSITION AND INVASION
. [Internet] [Doctoral dissertation]. Princeton University; 2019. [cited 2019 Dec 10].
Available from: http://arks.princeton.edu/ark:/88435/dsp01pk02cd52s.
Council of Science Editors:
Han S. BIOMECHANICAL REGULATION OF CELL PROLIFERATION, EPITHELIAL-MESENCHYMAL TRANSITION AND INVASION
. [Doctoral Dissertation]. Princeton University; 2019. Available from: http://arks.princeton.edu/ark:/88435/dsp01pk02cd52s
20.
Pettersson, Emelie.
Framgångsfaktorer vid implementering av Enterprise Content Management.
Degree: Informatics, 2016, University of Skövde
URL: http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-12500
► Enterprise Content Managment (ECM) är ett relativt nytt forskningsområde som fortfarande saknar vetenskaplig forskning (Alalwan & Weistroffer, 2012). Det finns ett uttryckt behov av…
(more)
▼ Enterprise Content Managment (ECM) är ett relativt nytt forskningsområde som fortfarande saknar vetenskaplig forskning (Alalwan & Weistroffer, 2012). Det finns ett uttryckt behov av att veta hur ECM system kan implementeras effektivt i verksamheter och detta har lett till att forskningsfrågan ” Vilka framgångsfaktorer finns vid implementering av Enterprise Content Management (ECM) system?” har formulerats. Genom en kvalitativ metodansats genomsöks tidigare publicerad litteratur som behandlar fältstudier för implementering av ECM system. I analyskapitlet presenteras två tabeller med de identifierade framgångsfaktorerna. Dessa har delats upp i två kategorier, organisatoriska och tekniska framgångsfaktorer, som kan resultera i en lyckad implementering av ECM system. Slutligen förs en diskussion som reflekterar över resultatet, vilka konsekvenser som framkommit från denna studie och var framtida forskning fortfarande behövs.
Enterprise Content Management (ECM) is a relatively new research area, which still lacks research (Alalwan & Weistroffer, 2012). There is an expressed need to know how ECM systems can be implemented effectively in organisations, and this has formulated the research question: “Which success factors can be found when implementing Enterprise Content Management (ECM) systems?”. By analysing previously published literature, to identify and compile the success factors that can be found in connection with implementation of ECM systems within organisations, a qualitative research method is used. In the analysis section two tables of identified success factors are presented. These have been divided in two categories, organizational and technological success factors, which can result in a successful implementation of an ECM system. Lastly, a discussion reflects upon the findings, on the implications this study brings into light and where future research is still needed.
Subjects/Keywords: Enterprise Content Management; ECM; success factors; information management; Enterprise Content Management; ECM; framgångsfaktorer; informationshantering; Information Systems; Systemvetenskap, informationssystem och informatik
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APA (6th Edition):
Pettersson, E. (2016). Framgångsfaktorer vid implementering av Enterprise Content Management. (Thesis). University of Skövde. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-12500
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Pettersson, Emelie. “Framgångsfaktorer vid implementering av Enterprise Content Management.” 2016. Thesis, University of Skövde. Accessed December 10, 2019.
http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-12500.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Pettersson, Emelie. “Framgångsfaktorer vid implementering av Enterprise Content Management.” 2016. Web. 10 Dec 2019.
Vancouver:
Pettersson E. Framgångsfaktorer vid implementering av Enterprise Content Management. [Internet] [Thesis]. University of Skövde; 2016. [cited 2019 Dec 10].
Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-12500.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Pettersson E. Framgångsfaktorer vid implementering av Enterprise Content Management. [Thesis]. University of Skövde; 2016. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-12500
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Queensland University of Technology
21.
Kuaha, Kunnika.
Environments for zonal cartilage tissue engineering.
Degree: 2015, Queensland University of Technology
URL: https://eprints.qut.edu.au/81992/
► Articular cartilage is a highly organized tissue with cellular and matrix properties that vary with depth zones. Regenerating this zonal organization has proven difficult in…
(more)
▼ Articular cartilage is a highly organized tissue with cellular and matrix properties that vary with depth zones. Regenerating this zonal organization has proven difficult in tissue-engineered cartilage to treat damaged cartilage. In this thesis, we evaluated the effects of culture environments that mimic aspects of the native cartilage environment on chondrocyte subpopulations. We found that decellularized cartilage matrix can improve zonal tissue-engineered cartilage. Also, chondrocytes respond to signals from bone cells and compressive stimulation in a zone-dependent manner. These results highlight the importance of a zone-specific environment to improve tissue-engineered cartilage in vitro.
Subjects/Keywords: Decellularized ECM; Cartilage tissue engineering; Chondrocyte subpopulations; Cartilage extracellular matrix (ECM); Zonal constructs; Osteoarthritis (OA); Environments for zonal cartilage tissue engineerin
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Export
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Manager
APA (6th Edition):
Kuaha, K. (2015). Environments for zonal cartilage tissue engineering. (Thesis). Queensland University of Technology. Retrieved from https://eprints.qut.edu.au/81992/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Kuaha, Kunnika. “Environments for zonal cartilage tissue engineering.” 2015. Thesis, Queensland University of Technology. Accessed December 10, 2019.
https://eprints.qut.edu.au/81992/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Kuaha, Kunnika. “Environments for zonal cartilage tissue engineering.” 2015. Web. 10 Dec 2019.
Vancouver:
Kuaha K. Environments for zonal cartilage tissue engineering. [Internet] [Thesis]. Queensland University of Technology; 2015. [cited 2019 Dec 10].
Available from: https://eprints.qut.edu.au/81992/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Kuaha K. Environments for zonal cartilage tissue engineering. [Thesis]. Queensland University of Technology; 2015. Available from: https://eprints.qut.edu.au/81992/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Brno University of Technology
22.
Dvořák, Pavel.
Příprava aminových plazmových polymerů a jejich interakce s ECM proteiny
.
Degree: 2019, Brno University of Technology
URL: http://hdl.handle.net/11012/173439
► Bakalářská práce se zabývá přípravou aminových plazmových polymerů, které byly připraveny v nízkotlakém pulzním radiofrekvenčním kapacitně vázaném výboji za použití monomeru cyklopropylaminu (CPA). Povrchy funkcionalizované…
(more)
▼ Bakalářská práce se zabývá přípravou aminových plazmových polymerů, které byly připraveny v nízkotlakém pulzním radiofrekvenčním kapacitně vázaném výboji za použití monomeru cyklopropylaminu (CPA). Povrchy funkcionalizované aminovou skupinou nalézají uplatnění jako platformy pro imobilizaci biomolekul. V této práci byla zkoumána interakce plazmových polymerů s
ECM proteiny, konkrétně s kolagenem.; The bachelor thesis deals with the preparation of amine plasma polymers which were prepared in low pressure radiofrequency capacitively coupled plasma discharge using cyclopropylamine (CPA) monomer. The amine functionalized surfaces find application as scaffolds for the immobilization of biomolecules. In this work, the interaction of plasma polymers with
ECM proteins, specifically with collagen, was investigated.
Advisors/Committee Members: Zajíčková, Lenka (advisor).
Subjects/Keywords: plazma;
PECVD;
ECM proteiny;
kolagen;
cyklopropylamin;
plazmová polymerizace;
plasma;
PECVD;
ECM proteins;
collagen;
cyclopropylamine;
plasma polymerization
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Dvořák, P. (2019). Příprava aminových plazmových polymerů a jejich interakce s ECM proteiny
. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/173439
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Dvořák, Pavel. “Příprava aminových plazmových polymerů a jejich interakce s ECM proteiny
.” 2019. Thesis, Brno University of Technology. Accessed December 10, 2019.
http://hdl.handle.net/11012/173439.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Dvořák, Pavel. “Příprava aminových plazmových polymerů a jejich interakce s ECM proteiny
.” 2019. Web. 10 Dec 2019.
Vancouver:
Dvořák P. Příprava aminových plazmových polymerů a jejich interakce s ECM proteiny
. [Internet] [Thesis]. Brno University of Technology; 2019. [cited 2019 Dec 10].
Available from: http://hdl.handle.net/11012/173439.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Dvořák P. Příprava aminových plazmových polymerů a jejich interakce s ECM proteiny
. [Thesis]. Brno University of Technology; 2019. Available from: http://hdl.handle.net/11012/173439
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Lund
23.
Löfvall, Henrik.
Development of Novel Therapies, Models, and Biomarkers
for Osteoclast-Related Diseases.
Degree: 2018, University of Lund
URL: http://lup.lub.lu.se/record/434f9124-20fb-4289-85e8-b1b9a2009a84
;
https://portal.research.lu.se/ws/files/51454792/Henrik_L_avhandling_komplett_inkl._omslag_utan_Pap_V.pdf
► Osteoclasts are derived from hematopoietic stem cells (HSCs) and form upon stimulation of osteoclast precursors by macrophage colony-stimulating factor (M-CSF) and receptor activator of nuclear…
(more)
▼ Osteoclasts are derived from hematopoietic stem
cells (HSCs) and form upon stimulation of osteoclast precursors by
macrophage colony-stimulating factor (M-CSF) and receptor activator
of nuclear factor κΒ ligand (RANKL). Osteoclasts resorb bone by
secreting hydrochloric acid, matrix metalloproteinases (MMPs) and
cathepsin K. Failure of bone resorption or bone formation results
in metabolic bone diseases. Osteopetrosis (excess bone) is caused
by mutations impairing osteoclastogenesis or osteoclast function,
and can only be treated with a hematopoietic stem cell (HSC)
transplant (HSCT). Infantile malignant osteopetrosis (IMO) is
typically lethal without HSCT. 50% of IMO patients have mutations
in TCIRG1, a component of the osteoclast V-ATPase that is crucial
for acidification. The first aim of this dissertation was to
develop a clinically applicable TCIRG1-targeting gene therapy, to
circumvent the need for HSC donors, and assess its efficacy and
safety.Osteoclasts have also been implicated as mediators of bone
and cartilage degradation in rheumatic diseases. The increased bone
degradation in rheumatoid arthritis and osteoarthritis has been
strongly linked to increased osteoclast presence and activity, but
the role of osteoclasts in the pathological degradation of
cartilage—and type II collagen, its main component—remains unclear.
Therefore, the second aim of this dissertation was to develop novel
models and biomarkers for the investigation of osteoclast-mediated
cartilage degradation.In Paper I, we demonstrated that a
proof-of-concept lentiviral vector, with a viral promoter, used to
transduce IMO HSCs only induces TCIRG1 expression in osteoclasts,
not in precursors or macrophages, and that a small percentage of
treated cells is sufficient to restore resorption. In Paper II, we
developed a clinically applicable vector, with a human promoter,
and demonstrated that transduced human IMO HSCs transplanted into
mice maintained the ability to generate functional osteoclasts ex
vivo long-term. These studies are promising progress for the
development of IMO gene therapy. In Paper III, we developed a mouse
model with human HSCs expressing human M-CSF—to bypass the
incompatibility of the human M-CSF receptor and mouse M-CSF—by
transducing the HSCs with a human M-CSF vector and transplanting
them into immunodeficient mice. These mice had improved human
monocyte and myeloid reconstitution, but human osteoclasts could
not be detected.In Paper IV, we developed a cell culture model of
human osteoclasts resorbing cartilage; using protease inhibitors
and the C2M biomarker of type II collagen degradation, we
demonstrated that cartilage resorption was mediated by MMPs and, to
some extent, cathepsin K. In Paper V, we developed the type II
collagen degradation biomarker assay GPDPLQ1237, targeting an
elongated version of the CTX-II neo-epitope that has been
speculated to be osteoclast- and cathepsin K-specific. Both MMPs
and cathepsin K contributed to GPDPLQ1237 release from cartilage
resorption, but only MMPs contributed to CTX-II…
Subjects/Keywords: Cell and Molecular Biology; Osteopetros; Biomarkör; Cellodling; Extracellulär matrix (ECM); Osteoklast; Osteoclast; Biomarker; Cell culture; Osteopetrosis; Gene Therapy; Extracellular matrix (ECM)
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Löfvall, H. (2018). Development of Novel Therapies, Models, and Biomarkers
for Osteoclast-Related Diseases. (Doctoral Dissertation). University of Lund. Retrieved from http://lup.lub.lu.se/record/434f9124-20fb-4289-85e8-b1b9a2009a84 ; https://portal.research.lu.se/ws/files/51454792/Henrik_L_avhandling_komplett_inkl._omslag_utan_Pap_V.pdf
Chicago Manual of Style (16th Edition):
Löfvall, Henrik. “Development of Novel Therapies, Models, and Biomarkers
for Osteoclast-Related Diseases.” 2018. Doctoral Dissertation, University of Lund. Accessed December 10, 2019.
http://lup.lub.lu.se/record/434f9124-20fb-4289-85e8-b1b9a2009a84 ; https://portal.research.lu.se/ws/files/51454792/Henrik_L_avhandling_komplett_inkl._omslag_utan_Pap_V.pdf.
MLA Handbook (7th Edition):
Löfvall, Henrik. “Development of Novel Therapies, Models, and Biomarkers
for Osteoclast-Related Diseases.” 2018. Web. 10 Dec 2019.
Vancouver:
Löfvall H. Development of Novel Therapies, Models, and Biomarkers
for Osteoclast-Related Diseases. [Internet] [Doctoral dissertation]. University of Lund; 2018. [cited 2019 Dec 10].
Available from: http://lup.lub.lu.se/record/434f9124-20fb-4289-85e8-b1b9a2009a84 ; https://portal.research.lu.se/ws/files/51454792/Henrik_L_avhandling_komplett_inkl._omslag_utan_Pap_V.pdf.
Council of Science Editors:
Löfvall H. Development of Novel Therapies, Models, and Biomarkers
for Osteoclast-Related Diseases. [Doctoral Dissertation]. University of Lund; 2018. Available from: http://lup.lub.lu.se/record/434f9124-20fb-4289-85e8-b1b9a2009a84 ; https://portal.research.lu.se/ws/files/51454792/Henrik_L_avhandling_komplett_inkl._omslag_utan_Pap_V.pdf
University of Nairobi
24.
Byegon, Isaiah K.
Determinants of private investment in Kenya:An empirical analysis
.
Degree: 2009, University of Nairobi
URL: http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/20744
► This study was motivated by the conviction that private investment is the key to economic growth and increasing it is one of the prerequisites for…
(more)
▼ This study was motivated by the conviction that private investment is the key to economic
growth and increasing it is one of the prerequisites for achieving a sustainable economic
growth. The study examines the determinants of private investment in Kenya over the period
1970-2007. Due to non-stationarity of the macroeconomic variables in the model and the
existence of a cointegrating relationship, an Error Correction Mechanism (ECM) was
employed. The estimated long-run results show that real GDP growth, real exchange rate and
broad money supply as a ratio of GDP have positive and significant influence on private
investment in Kenya. The impact of trade policy, domestic savings, real lending rates, foreign
aid and FDI were positively related to private investment but insignificant. Credit to the
private sector and dummy variable for political regimes have a negative and significant effect
on private investment. Public investment, real deposit rates, public debt, inflation, foreign
reserves and dummy variable for financial liberation was negatively related to private
investment but insignificant.
Given the positive impact of real public investment, the study suggests policies such as
allocating public sector resources to capital accumulation. Since real lending interest rates
have a significant positive effect, it is essential to maintain the financial liberalization status
and in order to maintain the important link between GDP and private investment, there is need
to expand the agricultural sector from rain fed agriculture to irrigated agriculture. There is also
equally need to increase the rate of industrialization sector for economic development.
The study may serve as a guide for economist, policy makers and researchers in applied
macro-economists on the effects of macroeconomic variables on private investment.
Subjects/Keywords: Private investment;
Empirical analysis;
Kenya;
Error Correction Mechanism (ECM);
Macro-economic
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Byegon, I. K. (2009). Determinants of private investment in Kenya:An empirical analysis
. (Thesis). University of Nairobi. Retrieved from http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/20744
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Byegon, Isaiah K. “Determinants of private investment in Kenya:An empirical analysis
.” 2009. Thesis, University of Nairobi. Accessed December 10, 2019.
http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/20744.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Byegon, Isaiah K. “Determinants of private investment in Kenya:An empirical analysis
.” 2009. Web. 10 Dec 2019.
Vancouver:
Byegon IK. Determinants of private investment in Kenya:An empirical analysis
. [Internet] [Thesis]. University of Nairobi; 2009. [cited 2019 Dec 10].
Available from: http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/20744.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Byegon IK. Determinants of private investment in Kenya:An empirical analysis
. [Thesis]. University of Nairobi; 2009. Available from: http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/20744
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
25.
Tsai, Yeong-sheng.
The Analysis of Influencing Factors on Taiwan's Excess Saving.
Degree: Master, Economics, 2010, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0902110-214947
► The existing literature on current accountâs analysis of influencing factors-related issues had had extensive research achievements, but they mostly stressed the discussion of influencing factors…
(more)
▼ The existing literature on current accountâs analysis of influencing factors-related issues had had extensive research achievements, but they mostly stressed the discussion of influencing factors from outside the economic body. To better understand and improve governmentâs ability of controlling variations in current account balance in order to suit the macro economic situation through the use of nimble interest and exchange rate policies, we employ the analytical tool to examine factors that influence Taiwan's excess saving during the period from 1987Q3 to 2009Q1. We modify the current account share of GDP regression by including interest rate, exchange rate and quarterly rate of inflation to reflect the effect of current account dynamics. Empirical evidences indicate that the coefficients of the long-run relationship are significantly crucial. We apply both the linear error-correction model (
ECM) and nonlinear smooth transition regression model to investigate the dynamics of current account vis-a-vis interest rate, exchange rate and the quarterly rate of inflation. and find supportings to the appropriateness of nonlinear smooth transition regression model. Furthermore, exchange rate was found having positive impact on current account balances. That is, a depreciation in the exchange rate would improve the current account and an appreciation, on the other hand, will worsen the current account. But the quarterly rate of inflation has a significantly negative effect, with an increase in the quarterly rate of inflation leading to a decrease in current account balances. Finally, from the long run cointegrating relationship, current account balances raises while the interest rate is decreasing, indicating there might exist income effect when interest rates fall. Because a fall in income reduces consumption, and increases saving, in turn, causes current account balances to rise, and vice versa. The empirical results shows all coefficientsâ sign can not only explain and interpret real economic phenomena, but also are consistent with theoretical expectations.
Advisors/Committee Members: Ching-Nun Lee (chair), Ming-Jang WENG (committee member), Hsiu-Hsin Ko (chair).
Subjects/Keywords: ECM; smooth transition regression; income effect; current account
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APA ·
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MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Tsai, Y. (2010). The Analysis of Influencing Factors on Taiwan's Excess Saving. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0902110-214947
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Tsai, Yeong-sheng. “The Analysis of Influencing Factors on Taiwan's Excess Saving.” 2010. Thesis, NSYSU. Accessed December 10, 2019.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0902110-214947.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Tsai, Yeong-sheng. “The Analysis of Influencing Factors on Taiwan's Excess Saving.” 2010. Web. 10 Dec 2019.
Vancouver:
Tsai Y. The Analysis of Influencing Factors on Taiwan's Excess Saving. [Internet] [Thesis]. NSYSU; 2010. [cited 2019 Dec 10].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0902110-214947.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Tsai Y. The Analysis of Influencing Factors on Taiwan's Excess Saving. [Thesis]. NSYSU; 2010. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0902110-214947
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Case Western Reserve University
26.
Chin, LiKang.
Tyramine Substituted-Hyaluronan Enriched Fascia for Rotator
Cuff Tendon Repair.
Degree: PhD, Biomedical Engineering, 2011, Case Western Reserve University
URL: http://rave.ohiolink.edu/etdc/view?acc_num=case1291410773
► Rotator cuff tendon injury is a debilitating health concern that affects more than 40% of the aging population. Despite advances in surgical treatment, the…
(more)
▼ Rotator cuff tendon injury is a debilitating
health concern that affects more than 40% of the aging population.
Despite advances in surgical treatment, the failure rate of rotator
cuff repairs ranges 20-90%. Naturally-occurring extracellular
matrices (ECMs) have been recently investigated as augmentation
scaffolds, but none has yet demonstrated both the appropriate
biological and mechanical properties. This
dissertation proposes to enrich fascia
ECM with high molecular
weight tyramine substituted-hyaluronan (TS-HA) for rotator cuff
repair. The central hypothesis is that TS-HA treatment will
decrease chronic inflammation without decreasing the time-zero or
post-implantation mechanical properties of fascia. The specific
aims are to develop a TS-HA treatment method and to evaluate the
host response and concomitant mechanical properties of treated
fascia in a rat abdominal wall model. TS-HA
treatment increased the HA content of fascia by an order of
magnitude to ~1% tissue weight. The incorporated HA was distributed
throughout the
ECM and, upon cross-linking, was retained as a
hydrogel network. Cross-linked TS-HA treated fascia exhibited an
increased macrophage and giant cell response and a lower density of
fibroblast-like cells than water treated controls. Treated fascia,
with or without cross-linking, exhibited a predominantly M2
pro-remodeling macrophage profile similar to water controls, which
is suggestive of constructive tissue remodeling. All grafts
exhibited a chronic lymphocytic response that is suggestive of an
immune response to the fascia xenograft. Fascia samples in all
groups demonstrated time-dependent decreases in mechanical
properties. Cross-linked TS-HA treated fascia exhibited a lower
toe-region elastic modulus and trended towards a higher transition
strain than water treated controls not only after implantation, but
also at time zero. These findings demonstrate
that HA augmentation can alter both the host response and the
mechanical properties of fascia
ECM. Contrary to the hypothesis,
the particular TS-HA treatment (at the concentration, molecular
weight, and tyramine substitution rate) employed in this
dissertation elicited a heightened macrophage and giant cell
response and a decrease in low-load elastic mechanical properties
compared to water treated fascia. This work provides a starting
point and guidance for the ongoing development of TS-HA enriched
fascia
ECM as an augmentation scaffold for rotator cuff
repair.
Advisors/Committee Members: Marchant, Roger (Committee Chair), Derwin, Kathleen (Advisor).
Subjects/Keywords: Biomedical Engineering; ECM (extracellular matrix); hyaluronan; biocompatibility; macrophage; tendon
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APA ·
Chicago ·
MLA ·
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Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Chin, L. (2011). Tyramine Substituted-Hyaluronan Enriched Fascia for Rotator
Cuff Tendon Repair. (Doctoral Dissertation). Case Western Reserve University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=case1291410773
Chicago Manual of Style (16th Edition):
Chin, LiKang. “Tyramine Substituted-Hyaluronan Enriched Fascia for Rotator
Cuff Tendon Repair.” 2011. Doctoral Dissertation, Case Western Reserve University. Accessed December 10, 2019.
http://rave.ohiolink.edu/etdc/view?acc_num=case1291410773.
MLA Handbook (7th Edition):
Chin, LiKang. “Tyramine Substituted-Hyaluronan Enriched Fascia for Rotator
Cuff Tendon Repair.” 2011. Web. 10 Dec 2019.
Vancouver:
Chin L. Tyramine Substituted-Hyaluronan Enriched Fascia for Rotator
Cuff Tendon Repair. [Internet] [Doctoral dissertation]. Case Western Reserve University; 2011. [cited 2019 Dec 10].
Available from: http://rave.ohiolink.edu/etdc/view?acc_num=case1291410773.
Council of Science Editors:
Chin L. Tyramine Substituted-Hyaluronan Enriched Fascia for Rotator
Cuff Tendon Repair. [Doctoral Dissertation]. Case Western Reserve University; 2011. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=case1291410773
Case Western Reserve University
27.
Xue, Feng.
Specific ECM Engagement Differentially Modulates T Cell
Cytoskeletal Reorganization By Rho GTPases.
Degree: MSs, Pathology, 2009, Case Western Reserve University
URL: http://rave.ohiolink.edu/etdc/view?acc_num=case1221770198
► When T cells adhere to the ECM, under certain circumstances they will polarize and exhibit the morphology of a migrating cell. We are investigating three…
(more)
▼ When T cells adhere to the
ECM, under certain
circumstances they will polarize and exhibit the morphology of a
migrating cell. We are investigating three specific questions: (a)
What environmental signals modulate T cell cytoskeletal
reorganization, especially vimentin, the intermediate filament? (b)
Which Rho GTPase is involved in vimentin polarization on discrete
ECM ligands, such as collagen type I and fibronectin? (c) What is
the mechanism and function of vimentin reorganization upon
engagement of distinct
ECM ligands? Our preliminary findings
demonstrate that (a) certain but not all inside-out and outside-in
signals lead to integrin activation that results in T cell
cytoskeletal rearrangement, particularly vimentin polarization, on
ECM ligands. (b) Rho GTPases differentially modulate vimentin
polarization when T cells adhere to distinct
ECM ligands. This work
sets a base for further investigation of the potential role
vimentin plays in lymphocyte migration and signaling.
Advisors/Committee Members: Ziats, Nicholas (Committee Chair), Levine, Alan (Advisor).
Subjects/Keywords: Integrins; ECM; fibronectin; CELL; PBT; RHO GTPASES; GTPASES
Record Details
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Xue, F. (2009). Specific ECM Engagement Differentially Modulates T Cell
Cytoskeletal Reorganization By Rho GTPases. (Masters Thesis). Case Western Reserve University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=case1221770198
Chicago Manual of Style (16th Edition):
Xue, Feng. “Specific ECM Engagement Differentially Modulates T Cell
Cytoskeletal Reorganization By Rho GTPases.” 2009. Masters Thesis, Case Western Reserve University. Accessed December 10, 2019.
http://rave.ohiolink.edu/etdc/view?acc_num=case1221770198.
MLA Handbook (7th Edition):
Xue, Feng. “Specific ECM Engagement Differentially Modulates T Cell
Cytoskeletal Reorganization By Rho GTPases.” 2009. Web. 10 Dec 2019.
Vancouver:
Xue F. Specific ECM Engagement Differentially Modulates T Cell
Cytoskeletal Reorganization By Rho GTPases. [Internet] [Masters thesis]. Case Western Reserve University; 2009. [cited 2019 Dec 10].
Available from: http://rave.ohiolink.edu/etdc/view?acc_num=case1221770198.
Council of Science Editors:
Xue F. Specific ECM Engagement Differentially Modulates T Cell
Cytoskeletal Reorganization By Rho GTPases. [Masters Thesis]. Case Western Reserve University; 2009. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=case1221770198
University of California – Irvine
28.
Phan, Anne Quy.
Regulation of Regenerative Responses by Factors in the Extracellular Matrix during Axolotl (Ambystoma mexicanum) Limb Regeneration.
Degree: Biological Sciences, 2014, University of California – Irvine
URL: http://www.escholarship.org/uc/item/7pb003b5
► Salamanders are unique among adult vertebrates in their ability to regenerate complex body structures after traumatic injury. Axolotl limb regeneration is a stepwise sequence of…
(more)
▼ Salamanders are unique among adult vertebrates in their ability to regenerate complex body structures after traumatic injury. Axolotl limb regeneration is a stepwise sequence of three requisite processes: (1) scarless wound healing to generate a regenerative wound epithelium, (2) blastema formation by migration, proliferation and dedifferentiation to create a mass of multipotent regeneration-competent progenitor cells, and (3) induction of pattern formation by interaction of cells with opposing positional information. The challenge to understanding how to induce and enhance regenerative ability in humans is to discover which of these processes fail in humans and why they fail.In axolotl regeneration, cells maintain a memory of their original position and use this `positional information' to accurately regenerate the lost pattern. The goal of this project was to elucidate the molecular mechanism of positional information in axolotl limb regeneration and determine if it is conserved in mammals. I used an in vivo gain-of-function assay for the requisite processes of axolotl limb regeneration (Accessory Limb Model) to determine the minimal components required to mediate positional information and induce de novo limb pattern.I found that cell-free anterior and posterior extracellular matrix (ECM) have position-specific signaling properties that either inhibit regeneration or induce de novo limb pattern. The position-specific functionality of the ECM is established by differential expression of heparan sulfate sulfotransferases during regeneration resulting in position-specific growth factor regulation. My findings indicate that heparan sulfates are both necessary and sufficient to mediate positional information in axolotl limb regeneration. Similarly, mouse ECM was also capable of inducing limb pattern in a position-specific, heparan sulfate-dependent, age-dependent manner. Using mammalian-derived, commercially available factors, I synthetically induced axolotl limb regeneration, thus demonstrating that the factors required for regeneration are not salamander-specific.This study demonstrates a specific novel mechanism for positional information in axolotl limb regeneration and establishes a crucial functional link between salamander regeneration and mammals. The ability to regulate axolotl regeneration with mammalian ECM has allowed identification of target mechanisms to induce regeneration in humans. In addition to the fields of regeneration, development, and glycobiology, this novel mechanism of positional information has implications to the fields of cancer biology and aging.
Subjects/Keywords: Biology; Developmental biology; axolotl; ECM; heparan sulfate; limb; regeneration; salamander
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CSE |
Export
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Manager
APA (6th Edition):
Phan, A. Q. (2014). Regulation of Regenerative Responses by Factors in the Extracellular Matrix during Axolotl (Ambystoma mexicanum) Limb Regeneration. (Thesis). University of California – Irvine. Retrieved from http://www.escholarship.org/uc/item/7pb003b5
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Phan, Anne Quy. “Regulation of Regenerative Responses by Factors in the Extracellular Matrix during Axolotl (Ambystoma mexicanum) Limb Regeneration.” 2014. Thesis, University of California – Irvine. Accessed December 10, 2019.
http://www.escholarship.org/uc/item/7pb003b5.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Phan, Anne Quy. “Regulation of Regenerative Responses by Factors in the Extracellular Matrix during Axolotl (Ambystoma mexicanum) Limb Regeneration.” 2014. Web. 10 Dec 2019.
Vancouver:
Phan AQ. Regulation of Regenerative Responses by Factors in the Extracellular Matrix during Axolotl (Ambystoma mexicanum) Limb Regeneration. [Internet] [Thesis]. University of California – Irvine; 2014. [cited 2019 Dec 10].
Available from: http://www.escholarship.org/uc/item/7pb003b5.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Phan AQ. Regulation of Regenerative Responses by Factors in the Extracellular Matrix during Axolotl (Ambystoma mexicanum) Limb Regeneration. [Thesis]. University of California – Irvine; 2014. Available from: http://www.escholarship.org/uc/item/7pb003b5
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of California – Berkeley
29.
Brownfield, Douglas Glenn.
Characterization of the Interface between the Mouse Mammary Epithelium and its Microenvironment during Morphogenesis.
Degree: Bioengineering, 2011, University of California – Berkeley
URL: http://www.escholarship.org/uc/item/2pf0d08b
► In characterizing the interface between the mammary epithelium and the microenvironment, it is necessary to first study specific components individually before interpreting the results in…
(more)
▼ In characterizing the interface between the mammary epithelium and the microenvironment, it is necessary to first study specific components individually before interpreting the results in a larger context. With this in mind, the cell-cell component of the interface was first studied with respect to self-organization. Since initial experiments showed significant variance in size and morphology, a unique methodology was employed combining a micropatterning approach that confined cells to a cylindrical geometry with an algorithm to quantify changes of cellular distribution over time in order to measure the ability of different cell types to self-organize relative to each other. Using normal human mammary epithelial cells enriched into pools of the two principal lineages, luminal and myoepithelial cells, experiments demonstrated that bilayered organization in mammary epithelium was driven mainly by lineage-specific differential E-cadherin expression, but that P-cadherin contributed specifically to organization of the myoepithelial layer. Disruption of the actomyosin network or of adherens junction proteins resulted in either prevention of bilayer formation or loss of preformed bilayers, consistent with continual sampling of the local microenvironment by cadherins. Together these data show that self-organization is an innate and reversible property of communities of normal adult human mammary epithelial cells.Next, the cell-extracellular matrix (ECM) component was considered, focusing on the role of extracellular stiffness in both functional differentiation as well as branching morphogenesis. Since functional differentiation of the mammary epithelium involves the production of milk proteins such as caseins, a fluorescent reporter construct with the mouse beta-casein promoter was used as a metric for functional differentiation. This system was used for studies in which extracellular stiffness was tightly controlled and cellular stiffness measured and demonstrated a strong association between extra- and intracellular elasticity as well as functional differentiation in mammary epithelial cells (MECs). A benchmark for biomimetic intracellular elasticity was empirically determined via atomic force microscopy (AFM) measurements on normal mammary epithelium and used in subsequent experiments as a marker for the range of normal intracellular elasticity. The results established that maintenance of beta-casein expression required both laminin signaling and a `soft' extracellular matrix, as is the case in normal tissues in vivo, and biomimetic intracellular elasticity, as is the case in primary mammary epithelial organoids. Conversely, two hallmarks of breast cancer development, stiffening of the extracellular matrix and loss of laminin signaling, led to the loss of beta-casein expression and non-biomimetic intracellular elasticity. Extracellular stiffness was further plummeted with respect to branching morphogenesis by utilizing a three-dimensional culture model composed of type collagen-I. AFM was used to measure local…
Subjects/Keywords: Developmental biology; Cellular biology; Biomechanics; branching morphogenesis; ECM; mammary gland
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CSE |
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Manager
APA (6th Edition):
Brownfield, D. G. (2011). Characterization of the Interface between the Mouse Mammary Epithelium and its Microenvironment during Morphogenesis. (Thesis). University of California – Berkeley. Retrieved from http://www.escholarship.org/uc/item/2pf0d08b
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Brownfield, Douglas Glenn. “Characterization of the Interface between the Mouse Mammary Epithelium and its Microenvironment during Morphogenesis.” 2011. Thesis, University of California – Berkeley. Accessed December 10, 2019.
http://www.escholarship.org/uc/item/2pf0d08b.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Brownfield, Douglas Glenn. “Characterization of the Interface between the Mouse Mammary Epithelium and its Microenvironment during Morphogenesis.” 2011. Web. 10 Dec 2019.
Vancouver:
Brownfield DG. Characterization of the Interface between the Mouse Mammary Epithelium and its Microenvironment during Morphogenesis. [Internet] [Thesis]. University of California – Berkeley; 2011. [cited 2019 Dec 10].
Available from: http://www.escholarship.org/uc/item/2pf0d08b.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Brownfield DG. Characterization of the Interface between the Mouse Mammary Epithelium and its Microenvironment during Morphogenesis. [Thesis]. University of California – Berkeley; 2011. Available from: http://www.escholarship.org/uc/item/2pf0d08b
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Ohio University
30.
Yuan, Ye.
Effect of alpha 2,6 Sialylation and Ionizing Radiation on
Integrin-mediated Cell Adhesion and Cell Cycle Arrest.
Degree: PhD, Chemistry and Biochemistry (Arts and
Sciences), 2016, Ohio University
URL: http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1478556861213286
► Cell adhesion is an important early step of cancer metastasis, yet the roles of sialylation in regulating integrin-mediated breast cancer cell adhesion in comparison to…
(more)
▼ Cell adhesion is an important early step of cancer
metastasis, yet the roles of sialylation in regulating
integrin-mediated breast cancer cell adhesion in comparison to
migration and invasion are not well-understood. The role of
sialylation on alpha5 beta1 and alpha2 beta1 integrins in the
regulation of adhesion between breast cancer cells and
extracellular matrix (
ECM) was studied. Our data showed that
alpha2, alpha5 and beta1 integrins had considerable alpha 2,6
sialylation on MDA-MB-231 cells, whereas signals from MCF-7 cells
were undetectable. Cleavage of alpha 2,6 sialylation increased
adhesion of MDA-MB-231 cells to
ECM, while adhesion of MCF-7 cells
was unaffected, consistent with the latter’s lack of endogenous
alpha 2,6 sialylated surface integrins. Neither surface expression
of alpha5 beta1 and alpha2 beta1 integrins, nor activated beta 1
integrin, changed in MDA-MB-231 cells after sialidase treatment.
However, sialidase treatment did not have significant impact on
migration or invasion of MDA-MB-231 cells.Integrins not only play
an important role in adhesion of cancer cells, but also have a
direct connection with ionizing radiation-induced atherosclerosis,
which is an adverse effect observed after radiotherapy. However,
minimal attention has been given to monocytes/macrophages, which
are exposed to the radiation at the same time. Under flow
conditions using a parallel plate flow chamber to mimic
physiological shear stress, we demonstrate here that the avidity
between very late antigen-4 (VLA-4) of RAW264.7 cells and its
ligand vascular cell adhesion molecule-1 (VCAM-1), was increased
after low dose (0.5 Gy), but was reduced after higher dose (5 Gy)
treatment of ionizing radiation. Treating the cells with free
radical scavenger N-acetyl-L-cysteine reduced the avidity between
RAW264.7 cells and VCAM-1 to a similar level. These results suggest
that ionizing radiation regulates adhesive interactions between
VLA-4 and VCAM-1, and that reactive oxygen species might function
as a regulator, for this increased adhesiveness but with altered
expression of integrin not play a major role. In addition to
regulation of cell adhesion, ionizing radiation (IR) is known to
induce cell cycle arrest which correlates with cell proliferation
and cell death. Recent studies connect IR with endoplasmic
reticulum (ER) stress, leading to activation of PKR-like
endoplasmic reticulum kinase (PERK) and subsequent phosphorylation
of eukaryotic translation initiation factor 2 alpha (eIF2a).
However, the contribution of PERK and eIF2a phosphorylation in
IR-induced G2/M arrest is incompletely understood. Here we report
that IR-induced eIF2a phosphorylation was predominantly mediated by
PERK and IR-induced G2/M arrest was blunted after totally
abolishing eIF2a phosphorylation. Our results reveal that basal
level of eIF2a phosphorylation is indispensable to maintain G2/M
arrest in response to IR.
Advisors/Committee Members: Wu, Shiyong (Advisor).
Subjects/Keywords: Biochemistry; cell adhesion; integrins; sialylation; ECM; ionizing radiation; cell cycle
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Yuan, Y. (2016). Effect of alpha 2,6 Sialylation and Ionizing Radiation on
Integrin-mediated Cell Adhesion and Cell Cycle Arrest. (Doctoral Dissertation). Ohio University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1478556861213286
Chicago Manual of Style (16th Edition):
Yuan, Ye. “Effect of alpha 2,6 Sialylation and Ionizing Radiation on
Integrin-mediated Cell Adhesion and Cell Cycle Arrest.” 2016. Doctoral Dissertation, Ohio University. Accessed December 10, 2019.
http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1478556861213286.
MLA Handbook (7th Edition):
Yuan, Ye. “Effect of alpha 2,6 Sialylation and Ionizing Radiation on
Integrin-mediated Cell Adhesion and Cell Cycle Arrest.” 2016. Web. 10 Dec 2019.
Vancouver:
Yuan Y. Effect of alpha 2,6 Sialylation and Ionizing Radiation on
Integrin-mediated Cell Adhesion and Cell Cycle Arrest. [Internet] [Doctoral dissertation]. Ohio University; 2016. [cited 2019 Dec 10].
Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1478556861213286.
Council of Science Editors:
Yuan Y. Effect of alpha 2,6 Sialylation and Ionizing Radiation on
Integrin-mediated Cell Adhesion and Cell Cycle Arrest. [Doctoral Dissertation]. Ohio University; 2016. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1478556861213286
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Open Access Theses and Dissertations
