subject:( Cryopreservation)

Boston University

1. Riemer, Rebecca. Improving referral rate of female cancer patients to reproductive endocrinology.

Degree: MS, Physician Assistant Program, 2019, Boston University

► BACKGROUND: There are currently an estimated 250,000 female cancer survivors of reproductive age living in the US. Loss of fertility is an issue many cancer… (more)

▼ BACKGROUND: There are currently an estimated 250,000 female cancer survivors of reproductive age living in the US. Loss of fertility is an issue many cancer survivors face after treatment, as all forms of cancer therapy can cause infertility. Methods to preserve fertility can be initiated prior to cancer therapy. These methods include embryo cryopreservation, oocyte cryopreservation, fertility sparing surgery, ovarian tissue cryopreservation, ovarian transposition, and medical therapy. LITERATURE REVIEW: Although the clinical guidelines state that oncologists should discuss the risk of infertility with every patient of reproductive age and should refer every patient who is interested in or ambivalent towards fertility preservation to reproductive endocrinologists, studies have shown that a significant proportion of female cancer patients report never receiving information about fertility. Even fewer female cancer patients are referred to reproductive endocrinologists for further discussion and/or potential treatment. PROPOSED PROJECT: Oncologists at Boston Medical Center will be recruited to participate in a study that measures the effect of an educational intervention on referral rate to reproductive endocrinology. The knowledge gained from the intervention will be assessed with a pre- and post-test. The proportion of female patients age 18-45 referred to reproductive endocrinology will be evaluated through the Electronic Medical Record System. The correlation between knowledge gain and change in referral rates will also be assessed. CONCLUSION: Fertility after cancer treatment is an essential issue to consider for young cancer survivors. These patients benefit from being referred to reproductive endocrinologists so that they can get information about fertility preservation and undergo treatment in a timely fashion. Improving and/or reinforcing oncologist knowledge about this topic will increase the rate at which they initiate this conversation and therefore the number of female patients who are referred to reproductive endocrinology. SIGNIFICANCE: Providing female cancer patients with information about and opportunities to undergo fertility preservation will maximize their options. This will lead to a higher quality of life after cancer therapy. Advisors/Committee Members: Kuohung, Wendy (advisor), Weinstein, John (advisor).

Subjects/Keywords: Oncology; Embryo cryopreservation; Fertility preservation; Oocyte cryopreservation

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APA (6^th Edition):

Riemer, R. (2019). Improving referral rate of female cancer patients to reproductive endocrinology. (Masters Thesis). Boston University. Retrieved from http://hdl.handle.net/2144/38686

Chicago Manual of Style (16^th Edition):

Riemer, Rebecca. “Improving referral rate of female cancer patients to reproductive endocrinology.” 2019. Masters Thesis, Boston University. Accessed April 11, 2021. http://hdl.handle.net/2144/38686.

MLA Handbook (7^th Edition):

Riemer, Rebecca. “Improving referral rate of female cancer patients to reproductive endocrinology.” 2019. Web. 11 Apr 2021.

Vancouver:

Riemer R. Improving referral rate of female cancer patients to reproductive endocrinology. [Internet] [Masters thesis]. Boston University; 2019. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/2144/38686.

Council of Science Editors:

Riemer R. Improving referral rate of female cancer patients to reproductive endocrinology. [Masters Thesis]. Boston University; 2019. Available from: http://hdl.handle.net/2144/38686

Oregon State University

2. Fry Davidson, Allyson. Optimization of cryoprotectant addition and removal procedures for vitrification of adherent mammalian cells.

Degree: PhD, Chemical Engineering, 2013, Oregon State University

URL: http://hdl.handle.net/1957/36912

► Cryopreservation of adherent cells may be advantageous for cell types that are difficult to preserve in suspension or when it is necessary to preserve characteristics… (more)

Subjects/Keywords: vitrification; Cells – Cryopreservation

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APA (6^th Edition):

Fry Davidson, A. (2013). Optimization of cryoprotectant addition and removal procedures for vitrification of adherent mammalian cells. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/36912

Chicago Manual of Style (16^th Edition):

Fry Davidson, Allyson. “Optimization of cryoprotectant addition and removal procedures for vitrification of adherent mammalian cells.” 2013. Doctoral Dissertation, Oregon State University. Accessed April 11, 2021. http://hdl.handle.net/1957/36912.

MLA Handbook (7^th Edition):

Fry Davidson, Allyson. “Optimization of cryoprotectant addition and removal procedures for vitrification of adherent mammalian cells.” 2013. Web. 11 Apr 2021.

Vancouver:

Fry Davidson A. Optimization of cryoprotectant addition and removal procedures for vitrification of adherent mammalian cells. [Internet] [Doctoral dissertation]. Oregon State University; 2013. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/1957/36912.

Council of Science Editors:

Fry Davidson A. Optimization of cryoprotectant addition and removal procedures for vitrification of adherent mammalian cells. [Doctoral Dissertation]. Oregon State University; 2013. Available from: http://hdl.handle.net/1957/36912

Oregon State University

3. Lusianti, Ratih E. Improving the post-thaw processing of cryopreserved red blood cells using a combined approach of mathematical modeling and microfluidics.

Degree: PhD, Chemical Engineering, 2014, Oregon State University

URL: http://hdl.handle.net/1957/46826

► This study lays the groundwork on potential techniques that could be employed to improve the post-thaw wash processing of cryopreserved human red blood cells. Transfusion… (more)

▼ This study lays the groundwork on potential techniques that could be employed to improve the post-thaw wash processing of cryopreserved human red blood cells. Transfusion of red blood cells is one of the most commonly practiced procedures in clinical medicine. Millions of red blood cell units are transfused to patients every year. The current method of preservation of red blood cells only allows a short refrigerated shelf life of 6 weeks, which causes logistical issues and frequent shortages during the time when donor availability is decreased. Cryopreserving red blood cells in the presence of 40% w/v glycerol extends the stable shelf life of red blood cells to 10 years. The long stable shelf life allows stockpiling of transfusable RBC units. Although this preservation method is clinically approved and routinely performed, the use of cryopreservation in blood banking is limited to only autologous and rare units. One of the main reasons for this limitation is the time consuming post-thaw wash process required to remove the glycerol before the unit can be used for transfusion. The need to plan the amount of washed units makes the use of cryopreserved red blood cells for unscheduled transfusion particularly challenging. In this work, we have attempted to improve the efficiency of the post-thaw removal process by using modern scientific approaches. Using the mathematical model for cell membrane transport, we have demonstrated that glycerol can be rapidly extracted from red blood cells without excessively damaging the cell membrane in a batch process. This rapid glycerol removal approach was then applied into a membrane based microfluidic platform for adaptation into an efficient continuous process. A mathematical model of the membrane based microfluidic device capable of predicting the transport of water and solutes was developed to assist in the design of an efficient deglycerolization process. Glycerol removal experiments using a prototype of the device validated model predictions and demonstrated successful partial continuous deglycerolization of cryopreserved red blood cells. Simulations generated using the mathematical model shows that it is possible to rapidly deglycerolize cryopreserved red blood cells in a continuous process to levels acceptable for transfusion. With the potential for efficient continuous post-thaw wash processing, it is hoped that cryopreserved red blood cells may become a more attractive option for use in clinical therapy Advisors/Committee Members: Higgins, Adam Z. (advisor), McGuire, Joseph (committee member).

Subjects/Keywords: Blood cells – Cryopreservation

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APA (6^th Edition):

Lusianti, R. E. (2014). Improving the post-thaw processing of cryopreserved red blood cells using a combined approach of mathematical modeling and microfluidics. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/46826

Chicago Manual of Style (16^th Edition):

Lusianti, Ratih E. “Improving the post-thaw processing of cryopreserved red blood cells using a combined approach of mathematical modeling and microfluidics.” 2014. Doctoral Dissertation, Oregon State University. Accessed April 11, 2021. http://hdl.handle.net/1957/46826.

MLA Handbook (7^th Edition):

Lusianti, Ratih E. “Improving the post-thaw processing of cryopreserved red blood cells using a combined approach of mathematical modeling and microfluidics.” 2014. Web. 11 Apr 2021.

Vancouver:

Lusianti RE. Improving the post-thaw processing of cryopreserved red blood cells using a combined approach of mathematical modeling and microfluidics. [Internet] [Doctoral dissertation]. Oregon State University; 2014. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/1957/46826.

Council of Science Editors:

Lusianti RE. Improving the post-thaw processing of cryopreserved red blood cells using a combined approach of mathematical modeling and microfluidics. [Doctoral Dissertation]. Oregon State University; 2014. Available from: http://hdl.handle.net/1957/46826

Louisiana State University

4. Cuevas Uribe, Rafael. A general approach for vitrification of fish sperm.

Degree: PhD, Environmental Sciences, 2011, Louisiana State University

URL: etd-08252011-163658 ; https://digitalcommons.lsu.edu/gradschool_dissertations/3597

► The goal of this project was to develop streamlined protocols that could be integrated into a standardized approach for vitrification of germplasm for all aquatic… (more)

▼ The goal of this project was to develop streamlined protocols that could be integrated into a standardized approach for vitrification of germplasm for all aquatic species. Vitrification (freezing by formation of “glass” rather than crystalline ice) is simple, fast, inexpensive, can be potentially used to preserve samples in the field, and offers new options for germplasm management especially appropriate for small fishes. Sperm were studied from freshwater fish (channel catfish Ictalurus punctatus), viviparous freshwater fish (green swordtail Xiphophorus hellerii), and marine fishes (spotted seatrout Cynoscion nebulosus, red snapper Lutjanus campechanus, red drum Sciaenops ocellatus, and southern flounder Paralichthys lethostigma). To reduce toxicity, combinations of cryoprotectants at reduced concentrations with incorporation of trehalose and polymers were used to enhance glass formation. For freezing, samples were suspended on 10-µL polystyrene loops and plunged into liquid nitrogen. Thawing was done at 24ºC using Hanks’ balanced salt solution at 300 mOsmol/kg for freshwater species, and seawater at 1,020 mOsmol/kg for marine species. Quality after vitrification was evaluated by sperm motility, membrane integrity and when possible fertility. Post-thaw motility of sperm in marine fishes was higher (as high as 70%) than in freshwater fishes (as high as 20%). The percentage of membrane-intact sperm for marine fishes was ~20% except for southern flounder (11%). For freshwater fishes, the percentage of membrane-intact sperm for swordtail was low (<12%) compared to channel catfish (~50%). Adaptations by marine fish to high osmotic pressures could explain the survival in the high cryoprotectant concentrations (40 – 60%) required for vitrification. This research yielded the first successful vitrification of sperm in these fishes and production of offspring from vitrified sperm in channel catfish, green swordtail, and southern flounder. Sperm vitrification offers an alternative approach to conventional cryopreservation for conservation of valuable genetic lineages, such as endangered species, model strains used in research, and improved farmed strains. Furthermore, sperm vitrification could be used to transport cryopreserved sperm from the field to the laboratory to expand genetic resources available for germplasm repositories. This technique could be utilized to reconstitute genetic lines, and as a new option for conservation biology in imperiled aquatic species.

Subjects/Keywords: germplasm; cryopreservation; conservation

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APA (6^th Edition):

Cuevas Uribe, R. (2011). A general approach for vitrification of fish sperm. (Doctoral Dissertation). Louisiana State University. Retrieved from etd-08252011-163658 ; https://digitalcommons.lsu.edu/gradschool_dissertations/3597

Chicago Manual of Style (16^th Edition):

Cuevas Uribe, Rafael. “A general approach for vitrification of fish sperm.” 2011. Doctoral Dissertation, Louisiana State University. Accessed April 11, 2021. etd-08252011-163658 ; https://digitalcommons.lsu.edu/gradschool_dissertations/3597.

MLA Handbook (7^th Edition):

Cuevas Uribe, Rafael. “A general approach for vitrification of fish sperm.” 2011. Web. 11 Apr 2021.

Vancouver:

Cuevas Uribe R. A general approach for vitrification of fish sperm. [Internet] [Doctoral dissertation]. Louisiana State University; 2011. [cited 2021 Apr 11]. Available from: etd-08252011-163658 ; https://digitalcommons.lsu.edu/gradschool_dissertations/3597.

Council of Science Editors:

Cuevas Uribe R. A general approach for vitrification of fish sperm. [Doctoral Dissertation]. Louisiana State University; 2011. Available from: etd-08252011-163658 ; https://digitalcommons.lsu.edu/gradschool_dissertations/3597

Louisiana State University

5. Hardin, Paige T. Vitrification of Immature and Mature Bovine Oocytes.

Degree: MS, Animal Sciences, 2016, Louisiana State University

URL: etd-07062016-103539 ; https://digitalcommons.lsu.edu/gradschool_theses/4293

► Vitrification is the latest technique used in cryopreservation, the ability to utilize this method with oocytes and embryos has become a valuable system. Vitrification has… (more)

Subjects/Keywords: Vitrification; Cryopreservation; IVF

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APA (6^th Edition):

Hardin, P. T. (2016). Vitrification of Immature and Mature Bovine Oocytes. (Masters Thesis). Louisiana State University. Retrieved from etd-07062016-103539 ; https://digitalcommons.lsu.edu/gradschool_theses/4293

Chicago Manual of Style (16^th Edition):

Hardin, Paige T. “Vitrification of Immature and Mature Bovine Oocytes.” 2016. Masters Thesis, Louisiana State University. Accessed April 11, 2021. etd-07062016-103539 ; https://digitalcommons.lsu.edu/gradschool_theses/4293.

MLA Handbook (7^th Edition):

Hardin, Paige T. “Vitrification of Immature and Mature Bovine Oocytes.” 2016. Web. 11 Apr 2021.

Vancouver:

Hardin PT. Vitrification of Immature and Mature Bovine Oocytes. [Internet] [Masters thesis]. Louisiana State University; 2016. [cited 2021 Apr 11]. Available from: etd-07062016-103539 ; https://digitalcommons.lsu.edu/gradschool_theses/4293.

Council of Science Editors:

Hardin PT. Vitrification of Immature and Mature Bovine Oocytes. [Masters Thesis]. Louisiana State University; 2016. Available from: etd-07062016-103539 ; https://digitalcommons.lsu.edu/gradschool_theses/4293

Oregon State University

6. Lusianti, Ratih E. Removal of cryoprotectant with the use of a microseparation device.

Degree: MS, Chemical Engineering, 2010, Oregon State University

URL: http://hdl.handle.net/1957/18804

► Cryoprotectants (CPAs) such as glycerol and dimethyl sulfoxide (DMSO) are commonly used during cryopreservation of cell based therapeutics. Although these additives are beneficial during freezing,… (more)

▼ Cryoprotectants (CPAs) such as glycerol and dimethyl sulfoxide (DMSO) are commonly used during cryopreservation of cell based therapeutics. Although these additives are beneficial during freezing, it is often desirable to remove them before infusion into a patient. Currently, the most common method for CPA removal is by centrifugation. This method is time consuming, labor intensive, and can also lead to significant cell losses. In this study, we investigate the possible use of a microseparation device for removal of CPAs from red blood cell suspensions. A mathematical model was developed to predict the CPA removal performance of the device and cell volume changes during the process. Experiments to ascertain the permeability properties of several different types of membranes of interest were conducted using the device. The resulting experimental values were then incorporated into the model to make CPA removal predictions. To assess the accuracy of the model predictions, glycerol removal experiments from solutions without red blood cells were carried out. Through comparison of the experimental data and the model predictions, it was found that the model could accurately predict CPA removal for membranes with sufficiently small pores where mass transfer is dominated by diffusion; but in membranes with larger pores where mass transfer is dominated by pressure driven flow, the model predicted values that are lower than what was obtained through experiments. The reason for this effect is the pressure discrepancy that was found between the pressure drop recorded during the experiment and the model predicted pressure drop. The model predicted pressure drop assumes ideal fluid flow condition whereas the actual conditions during the experiment indicates the presence of air bubbles trapped inside the channels, obstructing the flow of fluid and possibly altering the surface area available for mass transfer. Parametric studies using model simulations on the CPA removal performance of the membranes with smaller pores were conducted. Through parametric studies, CPA removal trends and cell volume changes during the process using the membranes of interest were better understood. The information gained from this study is useful for designing the next prototype of the microseparation device as well as for developing an optimal CPA removal protocol for red blood cell suspensions. Advisors/Committee Members: Higgins, Adam Z. (advisor), Jovanovic, Goran (committee member).

Subjects/Keywords: Cryopreservation of organs; tissues; etc.

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APA (6^th Edition):

Lusianti, R. E. (2010). Removal of cryoprotectant with the use of a microseparation device. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/18804

Chicago Manual of Style (16^th Edition):

Lusianti, Ratih E. “Removal of cryoprotectant with the use of a microseparation device.” 2010. Masters Thesis, Oregon State University. Accessed April 11, 2021. http://hdl.handle.net/1957/18804.

MLA Handbook (7^th Edition):

Lusianti, Ratih E. “Removal of cryoprotectant with the use of a microseparation device.” 2010. Web. 11 Apr 2021.

Vancouver:

Lusianti RE. Removal of cryoprotectant with the use of a microseparation device. [Internet] [Masters thesis]. Oregon State University; 2010. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/1957/18804.

Council of Science Editors:

Lusianti RE. Removal of cryoprotectant with the use of a microseparation device. [Masters Thesis]. Oregon State University; 2010. Available from: http://hdl.handle.net/1957/18804

Universiteit Utrecht

7. Willemse, B.R. Trypanosoma Evansi: An Experimental Infection In Mice.

Degree: 2007, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/40135

► The experiments were aimed at creating sufficient infectious material to infect three groups of sow with Trypanosoma evansi and to evaluate whether there is a… (more)

Subjects/Keywords: Diergeneeskunde; trypanosoma, evansi, cryopreservation

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APA (6^th Edition):

Willemse, B. R. (2007). Trypanosoma Evansi: An Experimental Infection In Mice. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/40135

Chicago Manual of Style (16^th Edition):

Willemse, B R. “Trypanosoma Evansi: An Experimental Infection In Mice.” 2007. Doctoral Dissertation, Universiteit Utrecht. Accessed April 11, 2021. http://dspace.library.uu.nl:8080/handle/1874/40135.

MLA Handbook (7^th Edition):

Willemse, B R. “Trypanosoma Evansi: An Experimental Infection In Mice.” 2007. Web. 11 Apr 2021.

Vancouver:

Willemse BR. Trypanosoma Evansi: An Experimental Infection In Mice. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2007. [cited 2021 Apr 11]. Available from: http://dspace.library.uu.nl:8080/handle/1874/40135.

Council of Science Editors:

Willemse BR. Trypanosoma Evansi: An Experimental Infection In Mice. [Doctoral Dissertation]. Universiteit Utrecht; 2007. Available from: http://dspace.library.uu.nl:8080/handle/1874/40135

Mississippi State University

8. Matheny, Kelli Lynn. Effects of resveratrol on post-thaw quality of stallion sperm.

Degree: MS, Animal and Dairy Sciences, 2014, Mississippi State University

URL: http://sun.library.msstate.edu/ETD-db/theses/available/etd-11022014-205429/ ;

► Current equine sperm cryopreservation methods fail to reliably prevent damages to important cellular structures such as the cell membrane and DNA. The objective of… (more)

Subjects/Keywords: stallion; sperm; cryopreservation; resveratrol

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APA (6^th Edition):

Matheny, K. L. (2014). Effects of resveratrol on post-thaw quality of stallion sperm. (Masters Thesis). Mississippi State University. Retrieved from http://sun.library.msstate.edu/ETD-db/theses/available/etd-11022014-205429/ ;

Chicago Manual of Style (16^th Edition):

Matheny, Kelli Lynn. “Effects of resveratrol on post-thaw quality of stallion sperm.” 2014. Masters Thesis, Mississippi State University. Accessed April 11, 2021. http://sun.library.msstate.edu/ETD-db/theses/available/etd-11022014-205429/ ;.

MLA Handbook (7^th Edition):

Matheny, Kelli Lynn. “Effects of resveratrol on post-thaw quality of stallion sperm.” 2014. Web. 11 Apr 2021.

Vancouver:

Matheny KL. Effects of resveratrol on post-thaw quality of stallion sperm. [Internet] [Masters thesis]. Mississippi State University; 2014. [cited 2021 Apr 11]. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-11022014-205429/ ;.

Council of Science Editors:

Matheny KL. Effects of resveratrol on post-thaw quality of stallion sperm. [Masters Thesis]. Mississippi State University; 2014. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-11022014-205429/ ;

Mississippi State University

9. Langhorne, Cecilia Jane. Developing assisted reproductive technologies for endangered North American amphibians.

Degree: PhD, Biochemistry, Molecular Biology, Entomology and Plant Pathology, 2016, Mississippi State University

URL: http://sun.library.msstate.edu/ETD-db/theses/available/etd-12182015-123843/ ;

► An alarming number of anuran (frog and toad) species are facing the threat of extinction in the wild. In efforts to address this conservation… (more)

▼ An alarming number of anuran (frog and toad) species are facing the threat of extinction in the wild. In efforts to address this conservation crisis, captive breeding programs are rapidly being established at zoos and research institutions worldwide. However, the captive management of anurans can be challenging, as their reproduction is a tightly regulated hormonal response to environmental stimuli, often unknown or absent in captivity. Consequently, ex-situ breeding efforts tend to be greatly hindered by a paucity of knowledge in anuran reproductive physiology and, for many species on the brink of extinction, time is running out. Assisted reproductive technologies (ARTs), such as exogenous hormone induction of gamete release, artificial fertilization for population augmentation, and cryopreservation for the long-term storage of genetics, have the potential to greatly enhance captive breeding efforts in lieu of natural breeding. Broadly, research aims were to develop assisted reproductive technologies for captive populations of the declining Southern Rocky Mountain boreal toad (Anaxyrus boreas boreas), the critically endangered Mississippi Gopher Frog (Lithobates captio sevosa) and Puerto Rican crested toad (Peltophryne lemur). Specific objectives were to a) trial the efficacy of exogenous hormone treatments on sperm release in male target species by characterizing spermiation response across time; b) investigate methods for increasing sperm longevity through cold-storage and cryopreservation techniques; c) ascertain motility recovery rates and functional capacity of cold-stored and frozen-thawed spermatozoa through artificial fertilization techniques, and; d) apply successfully developed ARTs to determine the feasibility of genetically linking in-situ and ex-situ populations of A. b. boreas, through artificial fertilization of male and female gametes from wild and captive toads, respectively. Research outcomes from this study include the successful development of exogenous hormone protocols, spermiation profiles and sperm cryopreservation techniques for all target species. Additionally, these studies enabled validation of an alternative method for increasing genetic diversity in captive anurans through in-situ-ex-situ gamete linkage. Overall, this research emphasizes the potential value of assisted reproductive technologies as conservation tools for supporting the recovery of endangered frog and toad species worldwide. Advisors/Committee Members: Andrew Kouba (chair), Scott Willard (chair).

Subjects/Keywords: amphibians; hormone; cryopreservation; sperm; conservation

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APA (6^th Edition):

Langhorne, C. J. (2016). Developing assisted reproductive technologies for endangered North American amphibians. (Doctoral Dissertation). Mississippi State University. Retrieved from http://sun.library.msstate.edu/ETD-db/theses/available/etd-12182015-123843/ ;

Chicago Manual of Style (16^th Edition):

Langhorne, Cecilia Jane. “Developing assisted reproductive technologies for endangered North American amphibians.” 2016. Doctoral Dissertation, Mississippi State University. Accessed April 11, 2021. http://sun.library.msstate.edu/ETD-db/theses/available/etd-12182015-123843/ ;.

MLA Handbook (7^th Edition):

Langhorne, Cecilia Jane. “Developing assisted reproductive technologies for endangered North American amphibians.” 2016. Web. 11 Apr 2021.

Vancouver:

Langhorne CJ. Developing assisted reproductive technologies for endangered North American amphibians. [Internet] [Doctoral dissertation]. Mississippi State University; 2016. [cited 2021 Apr 11]. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-12182015-123843/ ;.

Council of Science Editors:

Langhorne CJ. Developing assisted reproductive technologies for endangered North American amphibians. [Doctoral Dissertation]. Mississippi State University; 2016. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-12182015-123843/ ;

University of Alberta

10. Hahn, Joshua N. Optimization of Vitrification for Human Articular Cartilage.

Degree: MS, Department of Surgery, 2015, University of Alberta

URL: https://era.library.ualberta.ca/files/2b88qg163

► Osteoarthritis, a disease resulting in the breakdown of cartilage and bone within joints, is a global burden that is growing in scope. There is no… (more)

▼ Osteoarthritis, a disease resulting in the breakdown of cartilage and bone within joints, is a global burden that is growing in scope. There is no cure for osteoarthritis, and the current treatments are all lacking in some form or another. One treatment which attempts to prevent degeneration of cartilage injury into osteoarthritis is osteochondral allografting. This surgery involves the transplantation of healthy bone and cartilage to replace damaged and diseased areas. Osteochondral allografting application is limited primarily by the supply of fresh, healthy tissue and the lack of a long-term storage method that maintains cell viability within cartilage. Vitrification is a method of cryopreservation that preserves cells and tissues at temperatures low enough to halt all biological activity while maintaining cell health when applied properly. Previous work within this lab resulted in successful vitrification of human articular cartilage, but there is room for improvement. The current research was performed to explore the use of additive compounds as well as the use of a vitrification protocol with altered cryoprotectant exposure criteria in an attempt to improve the post-warmed health of the cryopreserved cartilage tissue. The use of chondroitin sulphate, tetramethylpyrazine, a combination of these two, ascorbic acid, and glucosamine was investigated in a set of cryoprotectant toxicity mitigation experiments. We found that when evaluating the effect of exposure to these compounds in a toxic cryoprotectant solution coupled with a two-day incubation, that all but the chondroitin sulphate alone were capable of improving tissue health, while there were no benefits seen when evaluated before the incubation period. The use of additive compounds has been shown to reduce long-term deleterious effects of CPA exposure, indicating that their use may be beneficial to a vitrification application due to the high CPA concentrations involved. This thesis also experimentally explored an altered cryoprotectant protocol proposed by another student, Nadia Shardt, who used Fick’s 1-D law of diffusion to determine the minimum time required for the diffusion of cryoprotectants into articular cartilage in concentrations that were adequate for vitrification. These modifications reduced the protocol length by one and a half hours, but did not result in viability results that were significantly improved over the standard protocol. As the experimental trials in this thesis work all produced a recovery cell viability that is much lower than the previously published results for the standard vitrification protocol, no conclusions can be made regarding which protocol to use based on these data. The experimental groups did not have an obvious deleterious effect on cell viability and, therefore, the reduction in protocol time may be beneficial. The standard vitrification protocol for articular cartilage has shown good results. The experiments performed here demonstrate that there are two potential avenues that may be exploited to enhance cell…

Subjects/Keywords: Articular Cartilage; Vitrification; Cryopreservation

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APA (6^th Edition):

Hahn, J. N. (2015). Optimization of Vitrification for Human Articular Cartilage. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/2b88qg163

Chicago Manual of Style (16^th Edition):

Hahn, Joshua N. “Optimization of Vitrification for Human Articular Cartilage.” 2015. Masters Thesis, University of Alberta. Accessed April 11, 2021. https://era.library.ualberta.ca/files/2b88qg163.

MLA Handbook (7^th Edition):

Hahn, Joshua N. “Optimization of Vitrification for Human Articular Cartilage.” 2015. Web. 11 Apr 2021.

Vancouver:

Hahn JN. Optimization of Vitrification for Human Articular Cartilage. [Internet] [Masters thesis]. University of Alberta; 2015. [cited 2021 Apr 11]. Available from: https://era.library.ualberta.ca/files/2b88qg163.

Council of Science Editors:

Hahn JN. Optimization of Vitrification for Human Articular Cartilage. [Masters Thesis]. University of Alberta; 2015. Available from: https://era.library.ualberta.ca/files/2b88qg163

University of Ottawa

11. Balcerzak, Anna. Elucidating the Key Structural Features of Carbohydrates and Surfactants Necessary for Inhibiting Ice Recrystallization .

Degree: 2014, University of Ottawa

URL: http://hdl.handle.net/10393/31768

► Ice recrystallization during thawing after cryopreservation results in extensive cellular damage that ultimately leads to cell death and decreased cell viabilities. This is a significant… (more)

▼ Ice recrystallization during thawing after cryopreservation results in extensive cellular damage that ultimately leads to cell death and decreased cell viabilities. This is a significant problem particularly with cryopreserved cells utilized in various regenerative medicine therapies. Given the success of these therapies to treat spinal cord injury, cartilage lesions, and cardiacdisease, the development of new and improved cryprotectants that minimize cell damageduring freeze-thawing and improve cell viability post-cryopreservation are urgently required. The current cryopreservative dimethyl sulfoxide, DMSO, is associated with cytotoxicity in clinical settings and is not an optimal cryopreservative. Our laboratory is interested in synthesizing small molecules that possess the property of ice recrystallization inhibition (IRI) activity that can be utilized as cryopreservatives without the cytotoxic effects associated with DMSO. This thesis focuses on the development of small molecule ice recrystallization inhibitors and elucidating the structural features of disaccharides and surfactants that are responsible for potent IRI activity. The first part of this study examines simple disaccharide derivatives mimicking those found in the native AFGP to determine whether disaccharide structure influences IRI activity. Towards this end, the (1,6)-linked AFGP disaccharide analogue was synthesized, assessed for IRI activity using a splat-cooling assay, and compared to the native (1,3)- and (1,4)-linked AFGP disaccharide analogues. The change in linkage was found to have a profound affect on IRI activity. The second part of the study focuses on surfactants and gelators as ice recrystallization inhibitors. Our laboratory has demonstrated that carbohydrate-based hydrogelators can be potent inhibitors of ice recrystallization. While our studies have indicated that a delicate balance between hydrophobic and hydrophilic interactions is crucial for ice recrystallization inhibition (IRI) activity, the essential structural features necessary for potent IRI activity remain unknown. To address this issue, structurally diverse amino acid-based surfactants/gelators, anti-ice nucleating agents, and glycoconjugates were synthesized and assessed for IRI activity. The results indicate that long alkyl chains and increased hydrophobicity are important for potent IRI activity and iii that the position of these alkyl chains is essential. Also, the counterion of these compounds affects the IRI activity and is related to the counterion degree of hydration. These compounds were assessed for their ability to cryopreserve human liver cells (Hep G2) and human bone marrow cells (Tf-1α) in cell-based assays. Additionally, the best IRI assay solution was determined, which involved studying how the salts of the phosphate buffered saline (PBS) solution modulated IRI activity. Finally, small molecule ice recrystallization inhibitors were assessed for their ability to protect the viral vectors vaccinia virus, vesicular stomatitis virus, and herpes simplex-1 virus…

Subjects/Keywords: ice recrystallization inhibition; cryopreservation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Balcerzak, A. (2014). Elucidating the Key Structural Features of Carbohydrates and Surfactants Necessary for Inhibiting Ice Recrystallization . (Thesis). University of Ottawa. Retrieved from http://hdl.handle.net/10393/31768

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Balcerzak, Anna. “Elucidating the Key Structural Features of Carbohydrates and Surfactants Necessary for Inhibiting Ice Recrystallization .” 2014. Thesis, University of Ottawa. Accessed April 11, 2021. http://hdl.handle.net/10393/31768.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Balcerzak, Anna. “Elucidating the Key Structural Features of Carbohydrates and Surfactants Necessary for Inhibiting Ice Recrystallization .” 2014. Web. 11 Apr 2021.

Vancouver:

Balcerzak A. Elucidating the Key Structural Features of Carbohydrates and Surfactants Necessary for Inhibiting Ice Recrystallization . [Internet] [Thesis]. University of Ottawa; 2014. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/10393/31768.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Balcerzak A. Elucidating the Key Structural Features of Carbohydrates and Surfactants Necessary for Inhibiting Ice Recrystallization . [Thesis]. University of Ottawa; 2014. Available from: http://hdl.handle.net/10393/31768

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Ottawa

12. Briard, Jennie Grace. The Rational Design and Use of Novel Small-Molecule Ice Recrystallization Inhibitors for the Cryopreservation of Hematopoietic Stem Cells and Red Blood Cells .

Degree: 2016, University of Ottawa

URL: http://hdl.handle.net/10393/35005

► Over the past few decades, there has been an increase in the development of new cellular therapies used for the treatment of various conditions. Thus,… (more)

▼ Over the past few decades, there has been an increase in the development of new cellular therapies used for the treatment of various conditions. Thus, the rapid development of therapies requiring transfusion and transplantation of cells has resulted in a need to preserve these cellular therapy products. Cryopreservation is the only currently used method for the long-term storage of cells. The most commonly used cryoprotectants are 10% dimethyl sulfoxide (DMSO) for hematopoietic stem cells (HSCs) and 40% glycerol for red blood cells (RBCs). DMSO fails to protect the functionality of HSCs after cryopreservation and therefore, up to 20% of HSC transplantations fail to engraft. The glycerol in thawed RBC units must be removed to <1% to prevent intravascular hemolysis which is time-consuming. Thus, there is an urgent need to develop improved cryoprotectants for HSCs and RBCs. DMSO and glycerol are unable to control ice recrystallization which is a major source of cellular injury during cryopreservation. Therefore, compounds with the ability to inhibit ice recrystallization could represent a new class of cryoprotectant with a novel mechanism of action. This thesis focuses on the rational design of small-molecule ice recrystallization inhibitors. The key structural attributes required for ice recrystallization inhibition (IRI) activity are investigated. The amphiphilic balance required for IRI activity is explored. Furthermore, two new classes of small-molecule IRIs containing aromatic rings were rationally designed. As a result, several very highly IRI active molecules were discovered. The use of IRIs to improve the cryopreservation of HSCs and RBCs was explored. A number of IRIs improved the post-thaw functionality of HSCs. Supplementation of the current cryoprotectant solution with IRIs resulted in an increase in CFU recovery and frequency of multipotent progenitors. This would reduce the percentage of engraftment failure and allow for a larger proportion of cord blood banks’ inventory to provide an adequate dose for patients requiring transplants. Several IRIs were found to be effective cryoprotectants for RBCs with reduced amounts of glycerol. This could reduce the deglycerolization time for RBCs. These results demonstrate the potential of small-molecule IRIs to improve the current cryopreservation procedures for important cellular therapy products.

Subjects/Keywords: ice recrystallization inhibition; cryopreservation

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APA (6^th Edition):

Briard, J. G. (2016). The Rational Design and Use of Novel Small-Molecule Ice Recrystallization Inhibitors for the Cryopreservation of Hematopoietic Stem Cells and Red Blood Cells . (Thesis). University of Ottawa. Retrieved from http://hdl.handle.net/10393/35005

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Briard, Jennie Grace. “The Rational Design and Use of Novel Small-Molecule Ice Recrystallization Inhibitors for the Cryopreservation of Hematopoietic Stem Cells and Red Blood Cells .” 2016. Thesis, University of Ottawa. Accessed April 11, 2021. http://hdl.handle.net/10393/35005.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Briard, Jennie Grace. “The Rational Design and Use of Novel Small-Molecule Ice Recrystallization Inhibitors for the Cryopreservation of Hematopoietic Stem Cells and Red Blood Cells .” 2016. Web. 11 Apr 2021.

Vancouver:

Briard JG. The Rational Design and Use of Novel Small-Molecule Ice Recrystallization Inhibitors for the Cryopreservation of Hematopoietic Stem Cells and Red Blood Cells . [Internet] [Thesis]. University of Ottawa; 2016. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/10393/35005.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Briard JG. The Rational Design and Use of Novel Small-Molecule Ice Recrystallization Inhibitors for the Cryopreservation of Hematopoietic Stem Cells and Red Blood Cells . [Thesis]. University of Ottawa; 2016. Available from: http://hdl.handle.net/10393/35005

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Gothenburg / Göteborgs Universitet

13. Milenkovic, Milan. Experimental studies on ovarian cryopreservation and transplantation.

Degree: 2011, University of Gothenburg / Göteborgs Universitet

URL: http://hdl.handle.net/2077/24323

► Around 8% of all cancer victims are below 40 years of age and the survival after cancer treatment during childhood and reproductive years has increased… (more)

▼ Around 8% of all cancer victims are below 40 years of age and the survival after cancer treatment during childhood and reproductive years has increased considerably to be around 80% today. The clinical field of fertility preservation has emerged to enable cancer patients that are treated with potentially gonadotoxic chemotherapy-radiotherapy during childhood or reproductive ages, to preserve their fertility. In prepubertal girls and women of reproductive age, where immediate IVF is not an option, ovarian cryopreservation and later re-transplantation is today the only fertility option. Today 13 live births have been reported worldwide after ovarian cortex cryopreservation and avascular re-transplantation some years after the woman has been declared disease-free. However, the effectiveness of the method of ovarian cryopreservation is low. This thesis investigates several models to be used in improvement of ovarian cryopreservation protocols, including whole ovary cryopreservation, and in addition studies different transplantation sites for avascular cortex transplantation in a non human primate species. The ovine ovarian ovary was used to examine a slow freezing method with the cryoprotectant dimethylsulphoxide (DMSO). Sheep ovaries were cryopreserved in liquid nitrogen and after thawing several viability tests were used. It was shown that the presence of DMSO was advantages for steroid and cyclic AMP output during in vitro perfusion and in cultured ovarian cells. Light microscopy showed well preserved tissue in the DMSO group after perfusion and a higher density of small follicles as compared to ovaries cryopreserved without of CPA. This study shows that the sheep ovary is a suitable method for further studies on whole ovary cryopreservation, including comparisons of different cryopreservation protocols. The human postmenopausal ovary was evaluated as a tool for further cryopreservation research in the human. Naturally cryopreservation of human ovaries is aiming at preserving premenopausal ovarian ovaries or ovarian tissue. However, this study on post menopausal ovary shows that the aged ovary can be used as a valuable tool for the research, with special emphasises on the function of the stroma and the vascularity. The study showed that human post menopausal ovaries could be effectively cryopreserved in DMSO and that the stroma secreted androgens during in vitro perfusion. Electron microscopy showed a well-preserved morphology in these human ovaries. The rodents are commonly used in reproductive physiology research and there is a large knowledge about the ovarian function and folliculogenesis in these species. The present study developed a technique for cannulation of the vasculature to the rat ovary and cryopreservation of the rat ovary by either vitrifiction or slow freezing. The cryoprotectant used was DMSO in high and low concentration. The result of the study indicated that a whole rat ovary can successfully be cryopreserved and that the DMSO concentration of 1.5 M is optimal when evaluating a secretion of…

Subjects/Keywords: cryopreservation; ovary; transplantation; animal models

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APA (6^th Edition):

Milenkovic, M. (2011). Experimental studies on ovarian cryopreservation and transplantation. (Thesis). University of Gothenburg / Göteborgs Universitet. Retrieved from http://hdl.handle.net/2077/24323

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Milenkovic, Milan. “Experimental studies on ovarian cryopreservation and transplantation.” 2011. Thesis, University of Gothenburg / Göteborgs Universitet. Accessed April 11, 2021. http://hdl.handle.net/2077/24323.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Milenkovic, Milan. “Experimental studies on ovarian cryopreservation and transplantation.” 2011. Web. 11 Apr 2021.

Vancouver:

Milenkovic M. Experimental studies on ovarian cryopreservation and transplantation. [Internet] [Thesis]. University of Gothenburg / Göteborgs Universitet; 2011. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/2077/24323.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Milenkovic M. Experimental studies on ovarian cryopreservation and transplantation. [Thesis]. University of Gothenburg / Göteborgs Universitet; 2011. Available from: http://hdl.handle.net/2077/24323

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Aberdeen

14. Moreira, Vanessa. Improving biosecurity of bovine in vitro embryo production and cryopreservation.

Degree: School of Medical Sciences., 2009, University of Aberdeen

URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=25708 ; http://digitool.abdn.ac.uk:1801/webclient/DeliveryManager?pid=25708&custom_att_2=simple_viewer

Subjects/Keywords: Cryopreservation.; Cattle

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APA (6^th Edition):

Moreira, V. (2009). Improving biosecurity of bovine in vitro embryo production and cryopreservation. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=25708 ; http://digitool.abdn.ac.uk:1801/webclient/DeliveryManager?pid=25708&custom_att_2=simple_viewer

Chicago Manual of Style (16^th Edition):

Moreira, Vanessa. “Improving biosecurity of bovine in vitro embryo production and cryopreservation.” 2009. Doctoral Dissertation, University of Aberdeen. Accessed April 11, 2021. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=25708 ; http://digitool.abdn.ac.uk:1801/webclient/DeliveryManager?pid=25708&custom_att_2=simple_viewer.

MLA Handbook (7^th Edition):

Moreira, Vanessa. “Improving biosecurity of bovine in vitro embryo production and cryopreservation.” 2009. Web. 11 Apr 2021.

Vancouver:

Moreira V. Improving biosecurity of bovine in vitro embryo production and cryopreservation. [Internet] [Doctoral dissertation]. University of Aberdeen; 2009. [cited 2021 Apr 11]. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=25708 ; http://digitool.abdn.ac.uk:1801/webclient/DeliveryManager?pid=25708&custom_att_2=simple_viewer.

Council of Science Editors:

Moreira V. Improving biosecurity of bovine in vitro embryo production and cryopreservation. [Doctoral Dissertation]. University of Aberdeen; 2009. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=25708 ; http://digitool.abdn.ac.uk:1801/webclient/DeliveryManager?pid=25708&custom_att_2=simple_viewer

Louisiana State University

15. Bailey, Cody Lee. Effects of Dietary Conjugated Linoleic Acid Supplementation on Bovine Oocyte Lipid Metabolism, Lipid Composition and Embryo Cryotolerance.

Degree: PhD, Animal Sciences, 2014, Louisiana State University

URL: etd-07072014-161918 ; https://digitalcommons.lsu.edu/gradschool_dissertations/846

► Variation in cryotolerance exists between embryos from different animal breed, species and management conditions. Reduced tolerance to chilling and cryotolerance of oocytes and embryos has… (more)

Subjects/Keywords: Holstein; Brahman; bovine embryo cryopreservation

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APA (6^th Edition):

Bailey, C. L. (2014). Effects of Dietary Conjugated Linoleic Acid Supplementation on Bovine Oocyte Lipid Metabolism, Lipid Composition and Embryo Cryotolerance. (Doctoral Dissertation). Louisiana State University. Retrieved from etd-07072014-161918 ; https://digitalcommons.lsu.edu/gradschool_dissertations/846

Chicago Manual of Style (16^th Edition):

Bailey, Cody Lee. “Effects of Dietary Conjugated Linoleic Acid Supplementation on Bovine Oocyte Lipid Metabolism, Lipid Composition and Embryo Cryotolerance.” 2014. Doctoral Dissertation, Louisiana State University. Accessed April 11, 2021. etd-07072014-161918 ; https://digitalcommons.lsu.edu/gradschool_dissertations/846.

MLA Handbook (7^th Edition):

Bailey, Cody Lee. “Effects of Dietary Conjugated Linoleic Acid Supplementation on Bovine Oocyte Lipid Metabolism, Lipid Composition and Embryo Cryotolerance.” 2014. Web. 11 Apr 2021.

Vancouver:

Bailey CL. Effects of Dietary Conjugated Linoleic Acid Supplementation on Bovine Oocyte Lipid Metabolism, Lipid Composition and Embryo Cryotolerance. [Internet] [Doctoral dissertation]. Louisiana State University; 2014. [cited 2021 Apr 11]. Available from: etd-07072014-161918 ; https://digitalcommons.lsu.edu/gradschool_dissertations/846.

Council of Science Editors:

Bailey CL. Effects of Dietary Conjugated Linoleic Acid Supplementation on Bovine Oocyte Lipid Metabolism, Lipid Composition and Embryo Cryotolerance. [Doctoral Dissertation]. Louisiana State University; 2014. Available from: etd-07072014-161918 ; https://digitalcommons.lsu.edu/gradschool_dissertations/846

Louisiana State University

16. Beehan, David Paul. The effects of iodixanol present during equine semen cryopreservation.

Degree: MS, Veterinary Medicine, 2012, Louisiana State University

URL: etd-11142012-084124 ; https://digitalcommons.lsu.edu/gradschool_theses/1316

► The objectives of this study were to determine what effects iodixanol would have on total and progressive motility, plasma membrane integrity (viability), acrosome integrity and… (more)

▼ The objectives of this study were to determine what effects iodixanol would have on total and progressive motility, plasma membrane integrity (viability), acrosome integrity and DNA structure when present during cryopreservation of equine spermatozoa,. We hypothesized that the addition of iodixanol would improve post-thaw values for measured parameters. Ejaculates from six stallions were collected, centrifuged at 900 x g for ten minutes to remove supernatant, and suspended to 200 x 106 cells/ml with 0%, 2.5% and 5% iodixanol in an egg-yolk based extender and cryopreserved. Before and after cryopreservation sperm motility was assessed by computer assisted semen analysis, and samples were stained with SYBR-14/propidium iodide (PI) for viability, with PI/fluorescent isothiocynate-PNA (Arachis Hypogaea) for acrosome integrity and assessed by flow cytometry. Sperm DNA was evaluated using the sperm chromatin structure assay test and assessed by flow cytometry. The mean (± S.E.) percentage pre- and post-thaw total motility, progressive motility, viability, acrosome reactivity, COMPát and MEANát were analyzed using Shapiro-Wilk test to evaluate if data followed a normal distribution. When results followed a normal distribution an ANOVA was performed and where a significant interaction of treatment was observed (p=0.05), a Tukey-Kramer post-hoc test was applied. If results did not follow a normal distribution, a binomial logistical regression was performed. The 5% post-thaw treatment group showed increased viability and decreased DNA damage (p<0.001). Although the 0% group showed greater total and progressive motility than the 2.5% group, it was not greater than the 5% group. The 5% post-thaw treatment group had significantly more (p<0.001) non-reacted and damaged acrosomes than both the 0% and 2.5% groups. These findings suggest that the presence of iodixanol during cryopreservation may have a beneficial effect by protecting plasma membrane and sperm DNA, but the exact mechanism of action is unknown.

Subjects/Keywords: Cryopreservation; Semen; Equine; Iodixanol

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APA (6^th Edition):

Beehan, D. P. (2012). The effects of iodixanol present during equine semen cryopreservation. (Masters Thesis). Louisiana State University. Retrieved from etd-11142012-084124 ; https://digitalcommons.lsu.edu/gradschool_theses/1316

Chicago Manual of Style (16^th Edition):

Beehan, David Paul. “The effects of iodixanol present during equine semen cryopreservation.” 2012. Masters Thesis, Louisiana State University. Accessed April 11, 2021. etd-11142012-084124 ; https://digitalcommons.lsu.edu/gradschool_theses/1316.

MLA Handbook (7^th Edition):

Beehan, David Paul. “The effects of iodixanol present during equine semen cryopreservation.” 2012. Web. 11 Apr 2021.

Vancouver:

Beehan DP. The effects of iodixanol present during equine semen cryopreservation. [Internet] [Masters thesis]. Louisiana State University; 2012. [cited 2021 Apr 11]. Available from: etd-11142012-084124 ; https://digitalcommons.lsu.edu/gradschool_theses/1316.

Council of Science Editors:

Beehan DP. The effects of iodixanol present during equine semen cryopreservation. [Masters Thesis]. Louisiana State University; 2012. Available from: etd-11142012-084124 ; https://digitalcommons.lsu.edu/gradschool_theses/1316

Louisiana State University

17. Scott, Brittany Reshel. Development and permeability of equine blastocysts.

Degree: MS, Animal Sciences, 2011, Louisiana State University

URL: etd-04262011-083851 ; https://digitalcommons.lsu.edu/gradschool_theses/2855

► Equine embryo cryopreservation is unsuccessful in larger, more easily collected, day-7 embryos. It is imperative that methods to successfully cryopreserve large equine embryos or develop… (more)

▼ Equine embryo cryopreservation is unsuccessful in larger, more easily collected, day-7 embryos. It is imperative that methods to successfully cryopreserve large equine embryos or develop reliable methods to determine embryo size before collection. Therefore the objectives for this study were to quantify the amount of tritiated glycerol that would permeate various sizes of equine embryos and to determine if circulating progesterone concentration was correlated with in utero embryo size. Mean embryo diameter (± SEM) across treatments (1.4M and 3.4M tritiated glycerol) was 696.5µm ± 108.6µm and 925.9 µm ± 214.1µm, respectively and were not different (P=0.44). The percent permeation for 1.4M and 3.4M glycerol were not different (P=0.68). Embryos <400 µm in the 1.4M glycerol treatment group had higher (P=0.002) permeation than embryos >400 µm, 8.32% ± 3.85% and 0.35% ± 0.11%, respectively. Length of time, 60 or 120 minutes, did not affect amount of glycerol uptaken (P=0.26. Serum progesterone concentrations on day 7 post-ovulation were higher (P=0.009) for mares who produced two viable embryos from double ovulation (24.17±2.82ng/ml) compared with mares from which a single embryo (14.04±0.99ng/ml) was collected and control mares (13.53±1.80ng/ml). No differences (P=0.91) were detected in serum progesterone concentration on day 7 post-ovulation between mares from which a single embryo (14.04±0.99ng/ml) was collected and control mares (13.53±1.80ng/ml). Mares producing embryos >400µm tended to have higher (P=0.08) circulating progesterone concentrations than mares producing embryos <400µm. Serum progesterone concentrations day 7 post-ovulation in mares producing embryos >400µm and <400µm were not different (P=0.61 and P=0.68, respectively) than control mares. Single embryos <1000µm in diameter were correlated with circulating progesterone concentration day 7 post-ovulation (r=0.46, P=0.006). There was no significant correlation between embryo diameter, corpus luteum diameter, and serum progesterone concentration day 7 post-ovulation.This is the first study to quantify the amount of glycerol permeating into equine blastocysts and suggests that the capsule may be a barrier to cryoprotectant permeability. Maternal progesterone concentrations day 7 post-ovulation could be utilized in predicting embryo stage and size prior to collection for cryopreservation and in diagnosis of twin pregnancies as a result of double ovulation.

Subjects/Keywords: capsule; cryopreservation; glycerol; horse; embryo

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Scott, B. R. (2011). Development and permeability of equine blastocysts. (Masters Thesis). Louisiana State University. Retrieved from etd-04262011-083851 ; https://digitalcommons.lsu.edu/gradschool_theses/2855

Chicago Manual of Style (16^th Edition):

Scott, Brittany Reshel. “Development and permeability of equine blastocysts.” 2011. Masters Thesis, Louisiana State University. Accessed April 11, 2021. etd-04262011-083851 ; https://digitalcommons.lsu.edu/gradschool_theses/2855.

MLA Handbook (7^th Edition):

Scott, Brittany Reshel. “Development and permeability of equine blastocysts.” 2011. Web. 11 Apr 2021.

Vancouver:

Scott BR. Development and permeability of equine blastocysts. [Internet] [Masters thesis]. Louisiana State University; 2011. [cited 2021 Apr 11]. Available from: etd-04262011-083851 ; https://digitalcommons.lsu.edu/gradschool_theses/2855.

Council of Science Editors:

Scott BR. Development and permeability of equine blastocysts. [Masters Thesis]. Louisiana State University; 2011. Available from: etd-04262011-083851 ; https://digitalcommons.lsu.edu/gradschool_theses/2855

Louisiana State University

18. Childress, William Martin. On-site Sperm Cryopreservation for Aquatic Repository Development and User Group Linkage.

Degree: MS, Environmental Sciences, 2017, Louisiana State University

URL: etd-05232017-085339 ; https://digitalcommons.lsu.edu/gradschool_theses/4577

► Although on-site cryopreservation of aquatic species sperm in nitrogen-vapor shipping dewars has been used for more than 30 yr, many limitations remain. Due to the… (more)

▼ Although on-site cryopreservation of aquatic species sperm in nitrogen-vapor shipping dewars has been used for more than 30 yr, many limitations remain. Due to the size of shipping dewars, most studies are small scale and can only produce tens of samples. The freezing temperatures that can be achieved are also affected by the size of the dewar and the number of samples being frozen due to the heat load. In addition, often due to timing or remote location these samples cannot be shipped, and if they are shipped, quality can be lost and the samples must be discarded. The goal of this thesis was to create a mobile aquatic cryopreservation laboratory that could operate at research and commercial-scale capacities and, could function under multiple scenarios in different on-site environments with a high level of quality control. There were three operational designs identified for the mobile laboratory: 1) self-contained work inside the unit using generator power; 2) work inside the unit using external facilities power; 3) using the equipment inside a host facility. Computer-aided design software was used to model a 3.8 m long trailer and various internal components to evaluate different configurations. From the final design, the layout inside the mobile laboratory was constructed and initially tested at the LSUAC Aquatic Germplasm and Genetic Resource Center. This demonstrated that the portable generator provided sufficient power for simultaneous use of all equipment. The capitol and variable costs were documented of construction and integration of the mobile laboratory into an existing cryopreservation facility at different levels of automation. This led to blueprints and layout information that can provide guidance for others to build their own units. Finally, the mobile laboratory was deployed into the field to cryopreserve sperm from Blue Catfish, Xiphophorus spp., and Red Snapper. This resulted in processing of 684 males and 6,838 French straw being produced. This work provides a general approach for high-throughput on-site cryopreservation of aquatic species for repository development and user group linkage. Germplasm repositories would allow the reconstitution of species or populations, and linking users could help remove standardization and quality control bottlenecks.

Subjects/Keywords: Cryopreservation; mobile; Aquatic; Germplasm

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APA (6^th Edition):

Childress, W. M. (2017). On-site Sperm Cryopreservation for Aquatic Repository Development and User Group Linkage. (Masters Thesis). Louisiana State University. Retrieved from etd-05232017-085339 ; https://digitalcommons.lsu.edu/gradschool_theses/4577

Chicago Manual of Style (16^th Edition):

Childress, William Martin. “On-site Sperm Cryopreservation for Aquatic Repository Development and User Group Linkage.” 2017. Masters Thesis, Louisiana State University. Accessed April 11, 2021. etd-05232017-085339 ; https://digitalcommons.lsu.edu/gradschool_theses/4577.

MLA Handbook (7^th Edition):

Childress, William Martin. “On-site Sperm Cryopreservation for Aquatic Repository Development and User Group Linkage.” 2017. Web. 11 Apr 2021.

Vancouver:

Childress WM. On-site Sperm Cryopreservation for Aquatic Repository Development and User Group Linkage. [Internet] [Masters thesis]. Louisiana State University; 2017. [cited 2021 Apr 11]. Available from: etd-05232017-085339 ; https://digitalcommons.lsu.edu/gradschool_theses/4577.

Council of Science Editors:

Childress WM. On-site Sperm Cryopreservation for Aquatic Repository Development and User Group Linkage. [Masters Thesis]. Louisiana State University; 2017. Available from: etd-05232017-085339 ; https://digitalcommons.lsu.edu/gradschool_theses/4577

19. Bandeira, Rafael dos Santos. Adição de piruvato e coenzima Q10 ao diluente à base de leite desnatado para refrigeração do sêmen equino.

Degree: 2019, Universidade Estadual Paulista (UNESP)

URL: http://hdl.handle.net/11449/182407

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Submitted by Rafael dos Santos Bandeira ([email protected]) on 2019-06-26T19:20:05Z No. of bitstreams: 1 Dissertação RAFAEL COMPLETA.pdf: 1224476 bytes, checksum: 29b3d7d4d41bca8966cd3b555d145b2e (MD5)

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Made available in DSpace on 2019-06-27T14:27:30Z (GMT). No. of bitstreams: 1 bandeira_rs_me_bot.pdf: 1224476 bytes, checksum: 29b3d7d4d41bca8966cd3b555d145b2e (MD5) Previous issue date: 2019-06-14

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

O espermatozoide exige um fornecimento constante de energia para a manutenção de suas funções celulares e quando desafiado por processos de criopreservação do sêmen, sofrem danos irreversíveis. Para o desenvolvimento de técnicas que visam o aumento da longevidade espermática, é necessário considerar que mesmo em metabolismo basal, o espermatozoide necessita de substratos para garantir sua motilidade e poder fecundante após a ejaculação. Para atender suas demandas energéticas, estudos recomendam o uso de nutrientes exógenos, como o piruvato de sódio e a coenzima Q10 (CoQ10), substratos fundamentais na bioenergética celular. Visto a importância da refrigeração de sêmen em garanhões e o potencial destas substâncias em melhorar os parâmetros seminais atuando como substrato energético e antioxidante, respectivamente, o presente trabalho tem por objetivo abordar aspectos relacionados ao metabolismo espermático, bem como o papel do piruvato e CoQ10 visando minimizar os efeitos deletérios da refrigeração sobre a qualidade do sêmen equino. Foram adicionadas diferentes concentrações de piruvato de sódio e da CoQ10 ao sêmen de garanhões considerados “good coolers” (GC) e “bad coolers” (BC). Primeiramente, foram estabelecidas as concentrações mais eficazes de piruvato e CoQ10 no diluente de refrigeração BotuSêmen® (Botupharma Botucatu/SP Brasil) para preservar os parâmetros espermáticos na refrigeração a 5° C por até 48 horas. Foi utilizado 1 ejaculado de 25 garanhões das raças Quarto de Milha e Mangalarga Marchador, cada ejaculado foi dividido em 7 frações, sendo uma parte destinada ao grupo controle (CT) e o restante dividido entre o piruvato de sódio e a CoQ10, adicionados nas concentrações de 1 mmol/l (P1), 2 mmol/l (P2), 3 mmol/l de piruvato de sódio (P3), 25 µmol/l (Q25), 50 µmol/l (Q50) e 75 µmol/l de coenzima Q10 (Q75) e submetidos ao processo de refrigeração a 5ºC. Na segunda etapa, foram utilizados 2 ejaculados de 7 garanhões considerados BC, bem como as concentrações mais eficazes de piruvato de sódio e CoQ10 e sua associação. Cada ejaculado foi dividido em 4 frações, sendo P1, Q25, associação de P1 + Q25 e o grupo controle (CT - BotuSêmen®). As amostras foram avaliadas quanto à cinética espermática, integridade de membrana plasmática, produção de espécies reativas ao oxigênio (H2O2 e O2-) e potencial…

Advisors/Committee Members: Universidade Estadual Paulista (UNESP), Dell'Aqua Júnior, José Antônio [UNESP].

Subjects/Keywords: antioxidante; sêmen; criopreservação; antioxidant; cryopreservation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bandeira, R. d. S. (2019). Adição de piruvato e coenzima Q10 ao diluente à base de leite desnatado para refrigeração do sêmen equino. (Masters Thesis). Universidade Estadual Paulista (UNESP). Retrieved from http://hdl.handle.net/11449/182407

Chicago Manual of Style (16^th Edition):

Bandeira, Rafael dos Santos. “Adição de piruvato e coenzima Q10 ao diluente à base de leite desnatado para refrigeração do sêmen equino.” 2019. Masters Thesis, Universidade Estadual Paulista (UNESP). Accessed April 11, 2021. http://hdl.handle.net/11449/182407.

MLA Handbook (7^th Edition):

Bandeira, Rafael dos Santos. “Adição de piruvato e coenzima Q10 ao diluente à base de leite desnatado para refrigeração do sêmen equino.” 2019. Web. 11 Apr 2021.

Vancouver:

Bandeira RdS. Adição de piruvato e coenzima Q10 ao diluente à base de leite desnatado para refrigeração do sêmen equino. [Internet] [Masters thesis]. Universidade Estadual Paulista (UNESP); 2019. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/11449/182407.

Council of Science Editors:

Bandeira RdS. Adição de piruvato e coenzima Q10 ao diluente à base de leite desnatado para refrigeração do sêmen equino. [Masters Thesis]. Universidade Estadual Paulista (UNESP); 2019. Available from: http://hdl.handle.net/11449/182407

Oregon State University

20. Uchendu, Esther Eyiuche. Cryopreservation of Shoot Tips: Antioxidant Investigations with Rubus and Protocols for Mentha and Vaccinium.

Degree: PhD, Horticulture, 2009, Oregon State University

URL: http://hdl.handle.net/1957/12731

► Oxidative stress that occurs during cryopreservation may be reduced by the addition of antioxidants. Vitamin E (Vit E), Vitamin C (Vit C), glutathione (GSH), lipoic… (more)

▼ Oxidative stress that occurs during cryopreservation may be reduced by the addition of antioxidants. Vitamin E (Vit E), Vitamin C (Vit C), glutathione (GSH), lipoic acid (LA), glycine betaine (GB) and polyvinylpyrrolidone (PVP) were applied at four steps of the PVS2 vitrification protocol (pretreatment, loading, rinsing, and regrowth). Shoot tips of in-vitro grown blackberry cultivars "Chehalem‟ and "Hull Thornless‟ were cryopreserved. Malondialdehyde (MDA), a lipid peroxidation product was higher in shoot tips at each step compared to controls. Shoot tips treated with Vit E had low MDA similar to the controls. GB, GSH, Vit E and Vit C significantly increased regrowth at all steps. Vit C added to regrowth medium with double Murashige and Skoog iron concentration decreased recovery; however, in iron-free medium Vit C improved regrowth. LA was not very effective in regrowth medium. PVP was not effective at any step. Regrowth was highest (up to 92%) with Vit C. This is the first report of vitamins C, and E, GB, and LA use in protecting cultures from oxidative damage during cryopreservation. Mentha species [M. canadensis L., M. australis R.Br., Mentha x piperita citrata (Ehrh.) Briq. and M. cunninghamii Benth,] were cryopreserved using controlled cooling (CC), encapsulation dehydration (ED), and PVS2 vitrification (VIT). Recovery of Mentha x piperita citrata and M. australis showed significant differences among protocols with CC > VIT > ED. M. canadensis and M. cunninghamii recovery with CC and ED was significantly better than VIT. All species responded to CC (93%) better than ED (71%) or VIT (73%). Cranberry (Vaccinium macrocarpon Aiton) cultivars "Wilcox‟ and "Franklin‟ and blueberry (Vaccinium corymbosum L.) "Berkeley‟, "O'Neal‟ and "Brigitta‟ were tested for desiccation tolerance and recovery after cryopreservation. Blueberry cultivars were desiccation tolerant after 7 h of drying under laminar air flow while cranberry cultivars were mostly dead by 3 h. Cryopreserved blueberry cultivars had 83% to 92% regrowth with ED. The results varied from 33% to 87% for VIT and from 50% to 67% for CC. Cranberry cultivars had poor regrowth with these protocols but improved by 20-30% with Vit C added at any of the four PVS2 steps. Advisors/Committee Members: Reed, Barbara M. (advisor), Chen, Tony H.H. (committee member).

Subjects/Keywords: Vitrification; Mints (Plants) – Germplasm resources – Cryopreservation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Uchendu, E. E. (2009). Cryopreservation of Shoot Tips: Antioxidant Investigations with Rubus and Protocols for Mentha and Vaccinium. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/12731

Chicago Manual of Style (16^th Edition):

Uchendu, Esther Eyiuche. “Cryopreservation of Shoot Tips: Antioxidant Investigations with Rubus and Protocols for Mentha and Vaccinium.” 2009. Doctoral Dissertation, Oregon State University. Accessed April 11, 2021. http://hdl.handle.net/1957/12731.

MLA Handbook (7^th Edition):

Uchendu, Esther Eyiuche. “Cryopreservation of Shoot Tips: Antioxidant Investigations with Rubus and Protocols for Mentha and Vaccinium.” 2009. Web. 11 Apr 2021.

Vancouver:

Uchendu EE. Cryopreservation of Shoot Tips: Antioxidant Investigations with Rubus and Protocols for Mentha and Vaccinium. [Internet] [Doctoral dissertation]. Oregon State University; 2009. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/1957/12731.

Council of Science Editors:

Uchendu EE. Cryopreservation of Shoot Tips: Antioxidant Investigations with Rubus and Protocols for Mentha and Vaccinium. [Doctoral Dissertation]. Oregon State University; 2009. Available from: http://hdl.handle.net/1957/12731

University of Alberta

21. Abazari Torqabeh, Alireza. Modeling the transport of cryoprotective agents in articular cartilage for cryopreservation.

Degree: PhD, Department of Chemical and Materials Engineering, 2010, University of Alberta

URL: https://era.library.ualberta.ca/files/h989r384n

► Loading vitrifiable concentrations of cryoprotective agents is an important step for cryopreservation of biological tissues by vitrification for research and transplantation purposes. This may be… (more)

▼ Loading vitrifiable concentrations of cryoprotective agents is an important step for cryopreservation of biological tissues by vitrification for research and transplantation purposes. This may be done by immersing the tissue in a cryoprotective agent (CPA) solution, and increasing the concentration, continuously or in multiple steps, and simultaneously decreasing the temperature to decrease the toxicity effects of the cryoprotective agent on the tissue cellular system. During cryoprotective agent loading, osmotic water movement from the tissue to the surrounding solution, and the resultant tissue shrinkage and stress-strain in the tissue matrix as well as on the cellular system can significantly alter the outcome of the cryopreservation protocol. In this thesis, a biomechanical model for articular cartilage is developed to account for the transport of the cryoprotective agent, the nonideal-nondilute properties of the vitrifiable solutions, the osmotic water movement and the resultant tissue shrinkage and stress-strain in the tissue matrix, and the osmotic volume change of the chondrocytes, during cryoprotective agent loading in the cartilage matrix. Four essential transport parameters needed for the model were specified, the values of which were obtained uniquely by fitting the model to experimental data from porcine articular cartilage. Then, it was shown that using real nonuniform initial distributions of water and fixed charges in cartilage, measured separately in this thesis using MRI, in the model can significantly affect the model predictions. The model predictions for dimethyl sulfoxide diffusion in porcine articular cartilage were verified by comparing to spatially and temporally resolved measurements of dimethyl sulfoxide concentration in porcine articular cartilage using a spectral MRI technique, developed for this purpose and novel to the field of cryobiology. It was demonstrated in this thesis that the developed mathematical model provides a novel tool for studying transport phenomena in cartilage during cryopreservation protocols, and can make accurate predictions for the quantities of interest for applications in the cryopreservation of articular cartilage.

Subjects/Keywords: Transport; Cryopreservation; Biomechanical model; Articular cartilage

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Abazari Torqabeh, A. (2010). Modeling the transport of cryoprotective agents in articular cartilage for cryopreservation. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/h989r384n

Chicago Manual of Style (16^th Edition):

Abazari Torqabeh, Alireza. “Modeling the transport of cryoprotective agents in articular cartilage for cryopreservation.” 2010. Doctoral Dissertation, University of Alberta. Accessed April 11, 2021. https://era.library.ualberta.ca/files/h989r384n.

MLA Handbook (7^th Edition):

Abazari Torqabeh, Alireza. “Modeling the transport of cryoprotective agents in articular cartilage for cryopreservation.” 2010. Web. 11 Apr 2021.

Vancouver:

Abazari Torqabeh A. Modeling the transport of cryoprotective agents in articular cartilage for cryopreservation. [Internet] [Doctoral dissertation]. University of Alberta; 2010. [cited 2021 Apr 11]. Available from: https://era.library.ualberta.ca/files/h989r384n.

Council of Science Editors:

Abazari Torqabeh A. Modeling the transport of cryoprotective agents in articular cartilage for cryopreservation. [Doctoral Dissertation]. University of Alberta; 2010. Available from: https://era.library.ualberta.ca/files/h989r384n

22. Rajan, Robin. Cryoprotective Properties of Completely Synthetic Polyampholytes via Reversible Addition-Fragmentation Chain Transfer (RAFT) Polymerization.

Degree: Japan Advanced Institute of Science and Technology / 北陸先端科学技術大学院大学

URL: http://hdl.handle.net/10119/11402

Supervisor:Associate Professor Kazuaki Matsumura

マテリアルサイエンス研究科

修士

Subjects/Keywords: Cryopreservation; Polyampholyte; RAFT

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rajan, R. (n.d.). Cryoprotective Properties of Completely Synthetic Polyampholytes via Reversible Addition-Fragmentation Chain Transfer (RAFT) Polymerization. (Thesis). Japan Advanced Institute of Science and Technology / 北陸先端科学技術大学院大学. Retrieved from http://hdl.handle.net/10119/11402

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rajan, Robin. “Cryoprotective Properties of Completely Synthetic Polyampholytes via Reversible Addition-Fragmentation Chain Transfer (RAFT) Polymerization.” Thesis, Japan Advanced Institute of Science and Technology / 北陸先端科学技術大学院大学. Accessed April 11, 2021. http://hdl.handle.net/10119/11402.

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rajan, Robin. “Cryoprotective Properties of Completely Synthetic Polyampholytes via Reversible Addition-Fragmentation Chain Transfer (RAFT) Polymerization.” Web. 11 Apr 2021.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

Rajan R. Cryoprotective Properties of Completely Synthetic Polyampholytes via Reversible Addition-Fragmentation Chain Transfer (RAFT) Polymerization. [Internet] [Thesis]. Japan Advanced Institute of Science and Technology / 北陸先端科学技術大学院大学; [cited 2021 Apr 11]. Available from: http://hdl.handle.net/10119/11402.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

Council of Science Editors:

Rajan R. Cryoprotective Properties of Completely Synthetic Polyampholytes via Reversible Addition-Fragmentation Chain Transfer (RAFT) Polymerization. [Thesis]. Japan Advanced Institute of Science and Technology / 北陸先端科学技術大学院大学; Available from: http://hdl.handle.net/10119/11402

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

University of Alberta

23. Jomha, Nadr Mohamed. Cryopreservation of intact human articular cartilage.

Degree: MS, Department of Surgery, 1996, University of Alberta

URL: https://era.library.ualberta.ca/files/3r074x65p

Subjects/Keywords: Articular cartilage – Cryopreservation.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jomha, N. M. (1996). Cryopreservation of intact human articular cartilage. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/3r074x65p

Chicago Manual of Style (16^th Edition):

Jomha, Nadr Mohamed. “Cryopreservation of intact human articular cartilage.” 1996. Masters Thesis, University of Alberta. Accessed April 11, 2021. https://era.library.ualberta.ca/files/3r074x65p.

MLA Handbook (7^th Edition):

Jomha, Nadr Mohamed. “Cryopreservation of intact human articular cartilage.” 1996. Web. 11 Apr 2021.

Vancouver:

Jomha NM. Cryopreservation of intact human articular cartilage. [Internet] [Masters thesis]. University of Alberta; 1996. [cited 2021 Apr 11]. Available from: https://era.library.ualberta.ca/files/3r074x65p.

Council of Science Editors:

Jomha NM. Cryopreservation of intact human articular cartilage. [Masters Thesis]. University of Alberta; 1996. Available from: https://era.library.ualberta.ca/files/3r074x65p

24. Sofoudis, Chrisostomos. Χρήση μικροσκοπίου φθορισμού για την εκτίμηση της επιβίωσης των ωοθυλακίων πρίν και μετά την κατάψυξη ωοθηκικού ιστού.

Degree: 2015, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/36664

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The annual incidence rate of cancer is estimated more than 11.000 patients in UK in the age group of 15-40 years old, which corresponds to… (more)

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The annual incidence rate of cancer is estimated more than 11.000 patients in UK in the age group of 15-40 years old, which corresponds to 4% of all cancer patients. The diagnosis of cancer is followed by devastating consequences for the patients themselves and their families in this age group. Although the treatment of cancer is of crucial significance, it should also examine the impact of the disease on fertility at the time of diagnosis and the damages caused from the surgical treatment, chemotherapy or radiotherapy. (Royal College of Obstetricians and Gynecologists, 2007)The gynecological cancer, the prevention and treatment, as well as the prevention of fertility in young women who are affected with the consequences of cancer remain the gold standard of the gynecologists. Infertility, whether temporary or permanent and premature menopause are common complications of the therapeutic approaches of cancer. The anticipated increase of the incidence of gynecological cancer in ever younger patients has led the medical community to specific efforts towards the prevention of fertility both through surgical practice and also through cryobiology, this scientific field, which studies the effect of low temperatures on living organisms. Namely cryobiology in terms of assisted reproduction, enables the process of cryopreservation of gametes, embryos and ovarian tissue.

Η ετήσια επίπτωση του καρκίνου υπολογίζεται σε πάνω από 11.000 ασθενείς στο Ηνωμένο Βασίλειο στην ηλικιακή ομάδα 15-40 ετών, η οποία αντιστοιχεί στο 4% των καρκινοπαθών ασθενών. Η διάγνωση του καρκίνου έχει καταστροφικές συνέπειες για τους ασθενείς και τις οικογένειές τους σε αυτή την ηλικιακή ομάδα. Αν και η θεραπεία του καρκίνου είναι υψίστης σημασίας, θα πρέπει να εξετάζεται επίσης η επίδραση της νόσου επί της γονιμότητας τη στιγμή της διάγνωσης και των βλαβών, οι οποίες προκαλούνται εκ της χειρουργικής θεραπείας, χημειοθεραπείας ή ακτινοθεραπείας. (Royal College of Obstetricians and Gynecologists, 2007).Ο γυναικολογικός καρκίνος, η πρόληψη, διάγνωση και θεραπεία αυτού καθώς και η διατήρηση της γονιμότητας σε γυναίκες που έχουν νοσήσει αποτελούν το gold standard των ιατρών γυναικολόγων. Η υπογονιμότητα, είτε παροδική είτε μόνιμη, καθώς και η πρόωρη εμμηνόπαυση αποτελούν συχνές επιπλοκές των θεραπευτικών προσεγγίσεων του καρκίνου. Η αναμενόμενη αύξηση της επίπτωσης των γυναικολογικών καρκίνων σε όλο και νεότερες ασθενείς έχει οδηγήσει την ιατρική κοινότητα στην προσπάθεια επίτευξης διατήρησης της γονιμότητας της γυναίκας τόσο μέσω χειρουργικής πρακτικής όσο και μέσω κρυοβιολογίας, του επιστημονικού αυτού κλάδου, ο οποίος μελετά την επίδραση των χαμηλών θερμοκρασιών σε ζώντες οργανισμούς. Δηλαδή η κρυοβιολογία σε ότι αφορά την υποβοηθούμενη αναπαραγωγή, επιτρέπει τη διαδικασία κρυοσυντήρησης γαμετών, εμβρύων, ωαρίων και ωοθηκικού ιστού.

Subjects/Keywords: Κατάψυξη ωοθηκικού ιστού; Cryopreservation of ovarian tissue

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sofoudis, C. (2015). Χρήση μικροσκοπίου φθορισμού για την εκτίμηση της επιβίωσης των ωοθυλακίων πρίν και μετά την κατάψυξη ωοθηκικού ιστού. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/36664

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sofoudis, Chrisostomos. “Χρήση μικροσκοπίου φθορισμού για την εκτίμηση της επιβίωσης των ωοθυλακίων πρίν και μετά την κατάψυξη ωοθηκικού ιστού.” 2015. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed April 11, 2021. http://hdl.handle.net/10442/hedi/36664.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sofoudis, Chrisostomos. “Χρήση μικροσκοπίου φθορισμού για την εκτίμηση της επιβίωσης των ωοθυλακίων πρίν και μετά την κατάψυξη ωοθηκικού ιστού.” 2015. Web. 11 Apr 2021.

Vancouver:

Sofoudis C. Χρήση μικροσκοπίου φθορισμού για την εκτίμηση της επιβίωσης των ωοθυλακίων πρίν και μετά την κατάψυξη ωοθηκικού ιστού. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2015. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/10442/hedi/36664.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sofoudis C. Χρήση μικροσκοπίου φθορισμού για την εκτίμηση της επιβίωσης των ωοθυλακίων πρίν και μετά την κατάψυξη ωοθηκικού ιστού. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2015. Available from: http://hdl.handle.net/10442/hedi/36664

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

25. Ariane FlÃvia do Nascimento. Motilidade espermÃtica de sÃmen de peixes criopreservado em diferentes meios avaliada por mÃtodo subjetivo e computadorizado.

Degree: 2008, UNIVERSIDADE FEDERAL DE LAVRAS

URL: http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=1530

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Pirapitinga (Piaractus brachypomus) and curimba (Prochilodus lineatus) are Characiforme fish species, of economic and ecological importance. The aims of this study were to (a) evaluate… (more)

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Pirapitinga (Piaractus brachypomus) and curimba (Prochilodus lineatus) are Characiforme fish species, of economic and ecological importance. The aims of this study were to (a) evaluate different freezing media for semen of both species; (b) compare post-thaw sperm motility subjectively evaluated under light microscope and computerized system SCAÂ for both species and (c) determine post-thaw sperm velocities by SCAÂ for both species and; (d) establish a correlation between the sperm velocities and the fertilization rates for curimba. Pirapitinga semen was diluted in four freezing media prepared with two extenders (glucose and BTSÂ) combined with two cryoprotectants (methylglycol and DMSO) and frozen nitrogen vapor. Semen was thawed, sperm motility was evaluated both subjectively under light microscope and objectively by computer system SCAÂ and the curvilinear velocity (VCL), average path (VAP) and straight line (VSL) were calculated. Curimba semen was diluted in two semen extenders (ACPÂ and glucose) combined with methylglycol as cryoprotectant and frozen in nitrogen vapor. Half of the samples were evaluated for motility and sperm velocity, as described for pirapitinga. The other half was evaluated for the capacity to fertilize fresh oocytes from three females. In both pirapitinga and curimba, there was no difference in post-thaw sperm motility evaluated either subjectively or by computer system. In pirapitinga, semen cryopreserved in glucose-methylglycol yielded higher motility (81%) and velocities compared to semen cryopreserved in the other media. In curimba, semen cryopreserved in ACPÂ yielded higher motility (84%) than semen cryopreserved in glucose (75%), however fertilization rates and sperm velocities were similar among samples cryopreserved in both extenders. A positive correlation was observed between fertilization rate and sperm velocity. The cryoprotectant methylglycol was more efficient than DMSO for pirapitinga semen, as observed in other Characiformes. Glucose is a simple extender, available in drugstores and, combined with methylglycol, is an excellent freezing medium for semen of both fish species studied here.

A pirapitinga (Piaractus brachypomus) e a curimba (Prochilodus lineatus) sÃo espÃcies de peixe da ordem Characiforme, de importÃncia econÃmica e ecolÃgica. Os objetivos do presente estudo foram (a) avaliar diferentes meios de congelamento para o sÃmen de ambas as espÃcies; (b) comparar a motilidade espermÃtica apÃs o descongelamento avaliada pelo mÃtodo subjetivo ao microscÃpio de luz, bem como pelo mÃtodo computadorizado SCAÂ, em ambas as espÃcies; (c) determinar as velocidades espermÃticas apÃs o descongelamento pelo mÃtodo computadorizado SCAÂ, em ambas as espÃcies e; (d) estabelecer uma correlaÃÃo entre as velocidades espermÃticas e a taxa de fertilizaÃÃo para a curimba. O sÃmen de pirapitinga foi diluido em quatro meios de congelamento preparados com dois diluidores (glicose e BTSÂ) combinados a dois crioprotetores (metilglicol e DMSO) e congelado em vapor de nitrogenio. O sÃmen foi…

Advisors/Committee Members: Ana Tereza de MendonÃa Viveiros, Marcelo Castro Leal, Paulo dos Santos Pompeu, Rilke Tadeu Fonseca de Freitas.

Subjects/Keywords: fish; CriopreservaÃÃo; sperm; peixe; cryopreservation; AQUICULTURA; sÃmen

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nascimento, A. F. d. (2008). Motilidade espermÃtica de sÃmen de peixes criopreservado em diferentes meios avaliada por mÃtodo subjetivo e computadorizado. (Thesis). UNIVERSIDADE FEDERAL DE LAVRAS. Retrieved from http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=1530

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Nascimento, Ariane FlÃvia do. “Motilidade espermÃtica de sÃmen de peixes criopreservado em diferentes meios avaliada por mÃtodo subjetivo e computadorizado.” 2008. Thesis, UNIVERSIDADE FEDERAL DE LAVRAS. Accessed April 11, 2021. http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=1530.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Nascimento, Ariane FlÃvia do. “Motilidade espermÃtica de sÃmen de peixes criopreservado em diferentes meios avaliada por mÃtodo subjetivo e computadorizado.” 2008. Web. 11 Apr 2021.

Vancouver:

Nascimento AFd. Motilidade espermÃtica de sÃmen de peixes criopreservado em diferentes meios avaliada por mÃtodo subjetivo e computadorizado. [Internet] [Thesis]. UNIVERSIDADE FEDERAL DE LAVRAS; 2008. [cited 2021 Apr 11]. Available from: http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=1530.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nascimento AFd. Motilidade espermÃtica de sÃmen de peixes criopreservado em diferentes meios avaliada por mÃtodo subjetivo e computadorizado. [Thesis]. UNIVERSIDADE FEDERAL DE LAVRAS; 2008. Available from: http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=1530

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

26. Rafael VenÃncio de AraÃjo. Semen cryopreservation of rainbow trout XX-males.

Degree: 2007, UNIVERSIDADE FEDERAL DE LAVRAS

URL: http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=511

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This study was carried out during July and August of 2005 and 2006. The aim of this study was to improve the cryopreservation techniques for… (more)

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This study was carried out during July and August of 2005 and 2006. The aim of this study was to improve the cryopreservation techniques for semen cryopreservation of sex-reversed rainbow trout males (XX-males). The first session of experiments, only semen collected under abdominal massage was used. In experiment 1, four semen extenders (glucose 5,4%, NaCl 0,9%, NaCl 1,2%-tris and BTSÂ) and the addition of egg yolk at 0 and 5% to the freezing media were tested. Then the effects of DMSO, ethylene glycol, methylglycol and methanol as cryoprotectants, semen dilution ratios of 1:3 and 1:7 (experiment 2), straw volumes of 0.25; 0.5 and 4.0 ml (experiment 3) and thawing at a water bath temperatures of 70ÂC/3s, 60ÂC/8s and 50ÂC/8s (experiment 4) on post-thaw sperm motility were evaluated. In experiment 5, post-thaw semen was used at different sperm:egg ratios (15x106 to 60x106) and eyed-egg rate was calculated after 196ÂC days. In all theses five experiments, semen was cryopreserved in nitrogen (N2) vapor at -170ÂC for 12-16 h, then transferred to a liquid N2 vessel. During the second session of experiments, only intratesticular semen obtained after male sacrifice was used. In experiment 1, semen was cryopreserved in one extender (glucose 5,4%, NaCl 0,9% or NaCl 1,2%-tris), egg yolk and DMSO according to the method described previously, and thawed at 70ÂC/3s or 35ÂC/16s. Then the pre-freezing incubation of semen diluted (1:0, 1:6 or 1:8, experiment 2; 1:5, 1:7 or 1:9, experiment 3) in seminal plasma and frozen in glucose or NaCl as extender (experiment 3), egg yolk and DMSO was tested. When semen was collected under abdominal massage, the highest post-thaw sperm motilities were observed in those samples diluted 1:3 in glucose, egg yolk and DMSO as freezing medium, cryopreserved in 0.5-ml straws and thawed at 70ÂC/3s. The highest eyed-egg rates were observed when sperm:egg ratio was above 30x106. When intratesticular semen was immediately cryopreserved without the incubation period, very low post-thaw sperm motility were observed. However, when intratesticular semen was diluted 1:6 in seminal plasma, incubated for 1:30h and cryopreserved in glucose, egg and DMSO, post-thaw sperm motility increased from 18% (without incubation) to 60% (after incubation). Based in these results we can conclude that semen of rainbow trout XX-males can be successfully cryopreserved in a freezing medium containing glucose, egg and DMSO, in 0.5-ml straws and thawed at 70ÂC/3s. If intratesticular semen is used, than the pre-freezing incubation period of 1:30h in seminal plasma is mandatory.

O estudo foi conduzido entre julho e agosto de 2005 e 2006, com o objetivo de aperfeiÃoar as tÃcnicas de criopreservaÃÃo do sÃmen de machos de truta arco-Ãris obtidos por inversÃo sexual (machos-XX). Inicialmente, apenas os peixes que liberavam sÃmen por pressÃo abdominal foram utilizados. No experimento 1, foram testados quatro diluidores de sÃmen (glicose 5,4%, NaCl 0,9%, NaCl 1,2%-tris e BTSÂ) e o efeito da adiÃÃo da gema de ovo aos diluidores. Depois, o…

Advisors/Committee Members: Rilke Tadeu Fonseca de Freitas, Yara Aiko Tabata, JosÃ Cleto da Silva Filho, Ana Tereza de MendonÃa Viveiros.

Subjects/Keywords: Trout; CriopreservaÃÃo; semen; ZOOTECNIA; Truta; SÃmen; cryopreservation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

AraÃjo, R. V. d. (2007). Semen cryopreservation of rainbow trout XX-males. (Thesis). UNIVERSIDADE FEDERAL DE LAVRAS. Retrieved from http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=511

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

AraÃjo, Rafael VenÃncio de. “Semen cryopreservation of rainbow trout XX-males.” 2007. Thesis, UNIVERSIDADE FEDERAL DE LAVRAS. Accessed April 11, 2021. http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=511.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

AraÃjo, Rafael VenÃncio de. “Semen cryopreservation of rainbow trout XX-males.” 2007. Web. 11 Apr 2021.

Vancouver:

AraÃjo RVd. Semen cryopreservation of rainbow trout XX-males. [Internet] [Thesis]. UNIVERSIDADE FEDERAL DE LAVRAS; 2007. [cited 2021 Apr 11]. Available from: http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=511.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

AraÃjo RVd. Semen cryopreservation of rainbow trout XX-males. [Thesis]. UNIVERSIDADE FEDERAL DE LAVRAS; 2007. Available from: http://bibtede.ufla.br/tede//tde_busca/arquivo.php?codArquivo=511

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Saskatchewan

27. Abrishami, Mahsa. Cryopreservation and xenografting of testis tissue.

Degree: 2009, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-06092009-204251

► The objective of this thesis was to investigate and expand the use of testis tissue xenografting as means of maintaining the developmental potential of donor… (more)

▼ The objective of this thesis was to investigate and expand the use of testis tissue xenografting as means of maintaining the developmental potential of donor testis tissue. The objective of the first study was to investigate the effect of donor age on spermatogenesis in canine testis tissue after xenografting into immunodeficient recipient mice. Fragments of testis tissue from 12 dogs of different ages were xenografted under the back skin of mice. Donors were categorized based on testis developmental status at the time of grafting into: less than four months (immature), four to six months (young), and greater than six months of age (adult). The grafts were evaluated at four, six or eight months post-grafting. At four months post-grafting, immature and young groups had higher graft recovery rates (92 ± 5.8 and 88 ± 4.4% versus 69 ± 3.5%; P = 0.001 and P = 0.001), graft weights (34 ± 8.1 and 32 ± 11.0 mg versus 7 ± 2.6 mg; P = 0.001 and P = 0.02), vesicular gland indices (1.1 ± 0.20 and 0.6 ± 0.18% versus 0.1 ± 0.03%; P < 0.0001 and P = 0.02), seminiferous tubule numbers (517 ± 114.8 and 364 ± 161.0 versus 10 ± 5.1; P < 0.0001 and P = 0.03), and larger seminiferous tubular diameters (140 ± 17.8 and 130 ± 3.4 µm versus 55 ± 21.9 µm; P = 0.003 and P = 0.001), compared to adult donor xenografts. Xenografts from immature donors maintained the growth and development for eight months, as exhibited by greater graft weights (17 ± 4.6 mg, P = 0.002), seminiferous tubule numbers (547 ± 210.3, P < 0.01) and tubular diameters (93 ± 15.9 µm, P < 0.0001), and induced greater vesicular gland indices (1.5 ± 0.46%, P = 0.0005), compared to adult donor xenografts. The growth and development of testis tissue xenografts from immature and young donors were not different after eight months (P > 0.05). Young donor xenografts had greater seminiferous tubule number and diameter compared to adult donor xenografts (P = 0.009 and P = 0.004, respectively) at eight months post-grafting. Elongated spermatids were the most advanced germ cell type present at four and eight months post-grafting in the testis grafts of immature and young age groups. The objective of the second study was to evaluate three different strategies to preserve/cryopreserve immature porcine testis tissue. Immature porcine testes were cooled at 4 °C for 24, 48 or 72 hours, and testis tissue fragments were cryopreserved using programmed slow freezing with dimethyl sulfoxide (DMSO), glycerol, or ethylene glycol, or vitrified using DMSO or glycerol at 5, 15 or 30 min exposure time. In vitro cell viability was determined by trypan blue exclusion, and in vivo developmental potential was evaluated by xenografting into immunodeficient mice. Compared to fresh tissue, short-term cooling of porcine testis tissue resulted in similar in vitro cell survival rates (93 ± 2.2% for fresh versus 95 ± 0.3, 93 ± 1.7 and 87 ± 4.3% after 24, 48 and 72 hours at 4 °C, respectively; P = 0.74) and in vivo development, with generation of elongated spermatids and sperm after four months of grafting.… Advisors/Committee Members: Honaramooz, A., Mapletoft, Reuben J., Lessard, Carl, Anzar, Muhammad, Singh, Baljit.

Subjects/Keywords: Testis; Cryopreservation; xenografting

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Abrishami, M. (2009). Cryopreservation and xenografting of testis tissue. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-06092009-204251

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Abrishami, Mahsa. “Cryopreservation and xenografting of testis tissue.” 2009. Thesis, University of Saskatchewan. Accessed April 11, 2021. http://hdl.handle.net/10388/etd-06092009-204251.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Abrishami, Mahsa. “Cryopreservation and xenografting of testis tissue.” 2009. Web. 11 Apr 2021.

Vancouver:

Abrishami M. Cryopreservation and xenografting of testis tissue. [Internet] [Thesis]. University of Saskatchewan; 2009. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/10388/etd-06092009-204251.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Abrishami M. Cryopreservation and xenografting of testis tissue. [Thesis]. University of Saskatchewan; 2009. Available from: http://hdl.handle.net/10388/etd-06092009-204251

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Johannesburg

28. Chatiza, Fungayi Primrose. Characterization of testes and functional evaluation of cryopreserved epididymal spermatozoa from three South African antelope species.

Degree: 2014, University of Johannesburg

URL: http://hdl.handle.net/10210/8791

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Ph.D. (Zoology)

This project involves a detailed study of three South African antelope species, springbok, impala and blesbok. The study investigates the origins of sperm… (more)

Subjects/Keywords: Cryopreservation of organs, tissues, etc. - Methodology; Blesbok - Germplasm resources - Cryopreservation - South Africa; Impala - Germplasm resources - Cryopreservation - South Africa; Springbok - Germplasm resources - Cryopreservation - South Africa; Game protection - South Africa

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chatiza, F. P. (2014). Characterization of testes and functional evaluation of cryopreserved epididymal spermatozoa from three South African antelope species. (Thesis). University of Johannesburg. Retrieved from http://hdl.handle.net/10210/8791

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chatiza, Fungayi Primrose. “Characterization of testes and functional evaluation of cryopreserved epididymal spermatozoa from three South African antelope species.” 2014. Thesis, University of Johannesburg. Accessed April 11, 2021. http://hdl.handle.net/10210/8791.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chatiza, Fungayi Primrose. “Characterization of testes and functional evaluation of cryopreserved epididymal spermatozoa from three South African antelope species.” 2014. Web. 11 Apr 2021.

Vancouver:

Chatiza FP. Characterization of testes and functional evaluation of cryopreserved epididymal spermatozoa from three South African antelope species. [Internet] [Thesis]. University of Johannesburg; 2014. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/10210/8791.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chatiza FP. Characterization of testes and functional evaluation of cryopreserved epididymal spermatozoa from three South African antelope species. [Thesis]. University of Johannesburg; 2014. Available from: http://hdl.handle.net/10210/8791

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Guelph

29. McGowan, Janine. VIABILITY ASSESSMENT AND CRYOPRESERVATION OF THE HONEY BEE (APIS MELLIFERA) PARASITE, NOSEMA CERANAE.

Degree: MS, Department of Environmental Biology, 2012, University of Guelph

URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3801

► Originally described from the Asian honey bee, Apis cerana, the microsporidian Nosema ceranae is an obligate, intracellular parasite that has recently been discovered infecting the… (more)

Subjects/Keywords: Apis; mellifera; Nosema; ceranae; viability; cryopreservation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

McGowan, J. (2012). VIABILITY ASSESSMENT AND CRYOPRESERVATION OF THE HONEY BEE (APIS MELLIFERA) PARASITE, NOSEMA CERANAE. (Masters Thesis). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3801

Chicago Manual of Style (16^th Edition):

McGowan, Janine. “VIABILITY ASSESSMENT AND CRYOPRESERVATION OF THE HONEY BEE (APIS MELLIFERA) PARASITE, NOSEMA CERANAE.” 2012. Masters Thesis, University of Guelph. Accessed April 11, 2021. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3801.

MLA Handbook (7^th Edition):

McGowan, Janine. “VIABILITY ASSESSMENT AND CRYOPRESERVATION OF THE HONEY BEE (APIS MELLIFERA) PARASITE, NOSEMA CERANAE.” 2012. Web. 11 Apr 2021.

Vancouver:

McGowan J. VIABILITY ASSESSMENT AND CRYOPRESERVATION OF THE HONEY BEE (APIS MELLIFERA) PARASITE, NOSEMA CERANAE. [Internet] [Masters thesis]. University of Guelph; 2012. [cited 2021 Apr 11]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3801.

Council of Science Editors:

McGowan J. VIABILITY ASSESSMENT AND CRYOPRESERVATION OF THE HONEY BEE (APIS MELLIFERA) PARASITE, NOSEMA CERANAE. [Masters Thesis]. University of Guelph; 2012. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3801

University of the Western Cape

30. Mafunda, Patrick Siyambulela. Aspects of the reproduction of male and female African penguins (Spheniscus demersus) with special reference to sperm biology and cryopreservation .

Degree: 2018, University of the Western Cape

URL: http://hdl.handle.net/11394/6551

► In the marine environment, penguins have been described as curators and serve a critical role in ecological balance. The African penguin (Spheniscus demersus) has undergone… (more)

Subjects/Keywords: African penguin; Cryopreservation; Sperm motility; Sphenisciformes; Species

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mafunda, P. S. (2018). Aspects of the reproduction of male and female African penguins (Spheniscus demersus) with special reference to sperm biology and cryopreservation . (Thesis). University of the Western Cape. Retrieved from http://hdl.handle.net/11394/6551

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mafunda, Patrick Siyambulela. “Aspects of the reproduction of male and female African penguins (Spheniscus demersus) with special reference to sperm biology and cryopreservation .” 2018. Thesis, University of the Western Cape. Accessed April 11, 2021. http://hdl.handle.net/11394/6551.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mafunda, Patrick Siyambulela. “Aspects of the reproduction of male and female African penguins (Spheniscus demersus) with special reference to sperm biology and cryopreservation .” 2018. Web. 11 Apr 2021.

Vancouver:

Mafunda PS. Aspects of the reproduction of male and female African penguins (Spheniscus demersus) with special reference to sperm biology and cryopreservation . [Internet] [Thesis]. University of the Western Cape; 2018. [cited 2021 Apr 11]. Available from: http://hdl.handle.net/11394/6551.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mafunda PS. Aspects of the reproduction of male and female African penguins (Spheniscus demersus) with special reference to sperm biology and cryopreservation . [Thesis]. University of the Western Cape; 2018. Available from: http://hdl.handle.net/11394/6551

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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Open Access Theses and Dissertations