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You searched for subject:( Centrosome attachment). Showing records 1 – 3 of 3 total matches.

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Penn State University

1. Gao, Zhizhen. DEPLETION OF NUCLEAR ENVELOPE COMPONENTS CAUSES A NOVEL CENTROSOME ATTACHMENT DEFECT.

Degree: MS, Biochemistry, Microbiology, and Molecular Biology, 2008, Penn State University

The attachment of the centrosome to the nucleus is essential for C. elegans development. Two genes, zyg-12 and sun-1, are essential for centrosome attachment; zyg-12 mutant and sun-1(RNAi) (also called mtf-1) embryos are defective in the attachment of both centrosomes in 100% of 1-cell embryos. Here we report that several lamin network proteins and nuclear import components are involved in centrosomal attachment. Disruption of C. elegans lamin frequently resulted in a single detached centrosome, while the second centrosome remained associated with the nucleus. Disrupting lamin-binding proteins by RNAi co-depletion of emr-1 (emerin) and lem-2 (MAN-1) or by RNAi of baf-1 (BAF) resulted in an identical single detached centrosome phenotype. In addition, the same phenotype was observed in C. elegans nucleoporin NPP-6 or importin-depleted embryos as well. The localization of ZYG-12 and SUN-1 to the nuclear envelope was normal in all of these RNAi-treated embryos. We conclude that the reason for centrosomal detachment in lamina network proteins RNAi embryos may be different from that of ZYG-12- or SUN-1- depleted embryos. All of the RNAi-treated embryos also show reduced nuclear size. We proposed that reduced nuclear size causes the single detached centrosome. We observed these RNAi embryos and wild-type as nuclear size increased during the cell cycle, a common event in pronuclear migration. As the cell cycle progresses, the detached centrosome was observed to frequently migrate to the sperm pronucleus and attach. This attachment occurred as the nuclei reached a reproducible size, suggesting a threshold of available nuclear surface area that is required for attachment. In embryos fertilized with anucleated sperm, the normal size female pronucleus can hold two even three centrosomes. However, the small female pronucleus can only capture one centrosome and the other one only moved slightly toward the nucleus. We conclude that the attachment of centrosome to the nucleus depends on the interaction between the nuclear surface area and the microtubules.

Subjects/Keywords: Lamin-binding proteins; Nuclear lamins; Nuclear size; Centrosome attachment; Nuclear Pores

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Gao, Z. (2008). DEPLETION OF NUCLEAR ENVELOPE COMPONENTS CAUSES A NOVEL CENTROSOME ATTACHMENT DEFECT. (Masters Thesis). Penn State University. Retrieved from https://etda.libraries.psu.edu/catalog/8654

Chicago Manual of Style (16th Edition):

Gao, Zhizhen. “DEPLETION OF NUCLEAR ENVELOPE COMPONENTS CAUSES A NOVEL CENTROSOME ATTACHMENT DEFECT.” 2008. Masters Thesis, Penn State University. Accessed December 06, 2019. https://etda.libraries.psu.edu/catalog/8654.

MLA Handbook (7th Edition):

Gao, Zhizhen. “DEPLETION OF NUCLEAR ENVELOPE COMPONENTS CAUSES A NOVEL CENTROSOME ATTACHMENT DEFECT.” 2008. Web. 06 Dec 2019.

Vancouver:

Gao Z. DEPLETION OF NUCLEAR ENVELOPE COMPONENTS CAUSES A NOVEL CENTROSOME ATTACHMENT DEFECT. [Internet] [Masters thesis]. Penn State University; 2008. [cited 2019 Dec 06]. Available from: https://etda.libraries.psu.edu/catalog/8654.

Council of Science Editors:

Gao Z. DEPLETION OF NUCLEAR ENVELOPE COMPONENTS CAUSES A NOVEL CENTROSOME ATTACHMENT DEFECT. [Masters Thesis]. Penn State University; 2008. Available from: https://etda.libraries.psu.edu/catalog/8654


Penn State University

2. Chen, Yi. IDENTIFYING ADDITIONAL CENTROSOME ATTACHMENT GENES AND CHARACTERIZING CENTROSOMAL LOCALIZATION OF ZYG-12.

Degree: MS, Genetics, 2008, Penn State University

The association between the centrosome and the nucleus is crucial for mitosis in the early Caenorhabditis elegans embryo. Four components are essential for this association: zyg-12, sun-1, microtubules and dynein complex (Reinsch and Karsenti, 1997; Malone et al., 2003). However, the molecular mechanism governing the centrosome-nucleus association is incompletely understood, and the question whether additional components are involved in this process has not been answered yet. To address this question, I performed two genetic screens to identify additional genes that may play roles in the centrosome-nucleus association. I found additional zyg-12-like genes in the suppressor screen on a centrosome-duplication gene zyg-1. I also identified suppressors that may genetically interact with zyg-12 through another suppressor screen on gene zyg-12. The preliminary characterizations of zyg-1 suppressors suggest that gene zyg-1 may have an unrevealed function in promoting the centrosome-nucleus association. My zyg-12 suppressor screen also provided strong evidence for the existence of the additional components involving in this process. In addition, to better understand the function of zyg-12 in the association, I investigated the mechanism of the centrosomal localization of ZYG-12. Using the microtubule binding assay, I tested the hypothesis that ZYG-12 interacts with microtubules to localize to the centrosome. However, ZYG-12 did not bind to microtubules in the assay, which suggested that it may localize to the centrosome through some other mechanism.

Subjects/Keywords: zyg-12; zyg-1; centrosome attachment; suppressor screen

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Chen, Y. (2008). IDENTIFYING ADDITIONAL CENTROSOME ATTACHMENT GENES AND CHARACTERIZING CENTROSOMAL LOCALIZATION OF ZYG-12. (Masters Thesis). Penn State University. Retrieved from https://etda.libraries.psu.edu/catalog/8651

Chicago Manual of Style (16th Edition):

Chen, Yi. “IDENTIFYING ADDITIONAL CENTROSOME ATTACHMENT GENES AND CHARACTERIZING CENTROSOMAL LOCALIZATION OF ZYG-12.” 2008. Masters Thesis, Penn State University. Accessed December 06, 2019. https://etda.libraries.psu.edu/catalog/8651.

MLA Handbook (7th Edition):

Chen, Yi. “IDENTIFYING ADDITIONAL CENTROSOME ATTACHMENT GENES AND CHARACTERIZING CENTROSOMAL LOCALIZATION OF ZYG-12.” 2008. Web. 06 Dec 2019.

Vancouver:

Chen Y. IDENTIFYING ADDITIONAL CENTROSOME ATTACHMENT GENES AND CHARACTERIZING CENTROSOMAL LOCALIZATION OF ZYG-12. [Internet] [Masters thesis]. Penn State University; 2008. [cited 2019 Dec 06]. Available from: https://etda.libraries.psu.edu/catalog/8651.

Council of Science Editors:

Chen Y. IDENTIFYING ADDITIONAL CENTROSOME ATTACHMENT GENES AND CHARACTERIZING CENTROSOMAL LOCALIZATION OF ZYG-12. [Masters Thesis]. Penn State University; 2008. Available from: https://etda.libraries.psu.edu/catalog/8651

3. Minn, Il. CENTROSOME ATTACHMENT TO THE NUCLEUS REQUIRES THE SUN-1 MEDIATED LOCALIZATION OF ZYG-12 TO THE OUTER MEMBRANE OF THE NUCLEAR ENVELOPE IN C. ELEGANS EMBRYOS.

Degree: PhD, Biochemistry, Microbiology, and Molecular Biology, 2008, Penn State University

SUN-1 and ZYG-12 are nuclear envelope proteins required for the centrosomal attachment to the nucleus in C. elegans embryo. We have shown that two isoforms of the ZYG-12, which contain KASH and transmembrane domains, depend on SUN-1 (also called MTF-1), to localize to the nuclear envelope. To map the domains of ZYG-12 necessary for SUN-1 mediated localization, we expressed C-terminal fragments of ZYG-12, which contain KASH and transmembrane domains. These C-terminal fragments are sufficient for nuclear envelope localization in a SUN-1 dependent manner. We have investigated the membrane topologies of ZYG-12 and SUN-1. Both epitope accessibility and fluorescence protease protection assays using in vivo nuclei in combination with proteinase K protection assays using microsomes revealed that ZYG-12 and SUN-1 are type II membrane proteins of the outer and inner membrane of the nuclear envelope, respectively. ZYG-12 directly interacts with SUN-1. The interaction occurs between the KASH domain of ZYG-12 and the internal domain of SUN-1, distinct from SUN domain. FRAP analyses using in vivo nuclei and expression of ZYG-12 and SUN-1 in HeLa cells revealed that SUN-1 confines and is sufficient for the localization of ZYG-12 to ONM which is contiguous with ER. Putative ATPases ASNA-1 and ASNA-2 are required for the cytokinesis of early C. elegans embryo. Mammalian ASNA-1 is known to be responsible for ER-localization of tail-anchored (TA) proteins. Double RNAi of C. elegans asna-1 and asna-2 did not affect the localizaion of TA protein ZYG-12. Interestingly the RNAi pheno-copied syn-4 mutants and syn-4 RNAi. SYN-4 is one of the syntaxin proteins of C. elegans and is a TA protein and required for the cytokinesis of early embryo. SYN-4 localizes to cell membrane. Whether ASNA-1 and ASNA-2 is responsible for SYN-1 membrane localization is to be examined.

Subjects/Keywords: Centrosome; SUN-1; ZYG-12; Attachment

…for the attachment of the centrosome and nucleus . 24 Figure 2-6… …Dynein is required for the attachment of the centrosome and nucleus 25 Figure 2-7. ZYG-12… …the attachment of the centrosome and nucleus …. 32 Figure 3-1. ZYG-12 has a mini KASH… …attachment of the centrosome to the nucleus in early C. elegans embryos. 15 The centrosome… …position inside cells, the mechanisms of centrosome attachment, positioning, and migration are… 

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Minn, I. (2008). CENTROSOME ATTACHMENT TO THE NUCLEUS REQUIRES THE SUN-1 MEDIATED LOCALIZATION OF ZYG-12 TO THE OUTER MEMBRANE OF THE NUCLEAR ENVELOPE IN C. ELEGANS EMBRYOS. (Doctoral Dissertation). Penn State University. Retrieved from https://etda.libraries.psu.edu/catalog/8570

Chicago Manual of Style (16th Edition):

Minn, Il. “CENTROSOME ATTACHMENT TO THE NUCLEUS REQUIRES THE SUN-1 MEDIATED LOCALIZATION OF ZYG-12 TO THE OUTER MEMBRANE OF THE NUCLEAR ENVELOPE IN C. ELEGANS EMBRYOS.” 2008. Doctoral Dissertation, Penn State University. Accessed December 06, 2019. https://etda.libraries.psu.edu/catalog/8570.

MLA Handbook (7th Edition):

Minn, Il. “CENTROSOME ATTACHMENT TO THE NUCLEUS REQUIRES THE SUN-1 MEDIATED LOCALIZATION OF ZYG-12 TO THE OUTER MEMBRANE OF THE NUCLEAR ENVELOPE IN C. ELEGANS EMBRYOS.” 2008. Web. 06 Dec 2019.

Vancouver:

Minn I. CENTROSOME ATTACHMENT TO THE NUCLEUS REQUIRES THE SUN-1 MEDIATED LOCALIZATION OF ZYG-12 TO THE OUTER MEMBRANE OF THE NUCLEAR ENVELOPE IN C. ELEGANS EMBRYOS. [Internet] [Doctoral dissertation]. Penn State University; 2008. [cited 2019 Dec 06]. Available from: https://etda.libraries.psu.edu/catalog/8570.

Council of Science Editors:

Minn I. CENTROSOME ATTACHMENT TO THE NUCLEUS REQUIRES THE SUN-1 MEDIATED LOCALIZATION OF ZYG-12 TO THE OUTER MEMBRANE OF THE NUCLEAR ENVELOPE IN C. ELEGANS EMBRYOS. [Doctoral Dissertation]. Penn State University; 2008. Available from: https://etda.libraries.psu.edu/catalog/8570

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