subject:( Anatomy)

University of California – Irvine

1. Gallent, Erin Ann. Approaches to promoting mTOR-mediated growth and regeneration in the mature central nervous system.

Degree: Biomedical Sciences, 2018, University of California – Irvine

URL: http://www.escholarship.org/uc/item/98c4k608

► Regeneration of the injured adult central nervous system is limited. The phosphatase and tensin homolog on chromosome ten (PTEN) gene has been shown to enable… (more)

▼ Regeneration of the injured adult central nervous system is limited. The phosphatase and tensin homolog on chromosome ten (PTEN) gene has been shown to enable axonal regeneration in optic nerve crush and spinal cord injury models following neonatal conditional genetic deletion of PTEN (Park et al., 2008; Liu et al., 2010). As a follow-up to these experiments, I assessed the long-term histological and behavioral consequences of neonatal PTEN deletion (Gutilla et al., 2016). In this study, I observed massive cell body enlargement of the neurons that give rise to the major descending voluntary motor tract in the spinal cord (called cortical motoneurons, or CMNs). This discovery led us to hypothesize that deletion of PTEN during adulthood would also result in neuronal growth. Using two-photon microscopy and electron microscopy I found that adult PTEN deletion in neurons results in significant cell body and axonal growth, and that these enlarged neurons continue to express markers of growth-promoting pathways throughout life. To gain a better understanding of the morphological consequences of PTEN deletion in adult neurons, I imaged thick sections (200µm) containing fluorescently labeled PTEN deleted and control neurons in 3D using a modified CLARITY protocol and two-photon microscopy (Chung and Deisseroth, 2013). 3D images were then used to generate 2D projections that were processed using Sholl analysis, or concentric circle analysis (Sholl, 1953). We found that at both 6 and 12 months post-PTEN deletion, pyramidal neurons have a significantly higher density of dendrites, and that this increase is significant at the largest number of radii away from the soma at 12 months after deletion. The progressive changes in dendritic morphology were also seen in the number of basal dendrites projecting off of the soma number, maximal dendritic projections, and nodes, with the highest values for all three measurements seen at 12 months, not 6 months, post-deletion. What’s more, the radius of maximal intersection for pyramidal neurons is normally between 60-80 µm away from the soma. Only at 12 months post-deletion did pyramidal neurons exhibit a shift in the radius of maximal intersection, indicating that as PTEN deletion-induced changes in morphology progress over time, neurons becomes increasingly abnormal compared to controls.To assess the effect of adult PTEN deletion on neuronal function and behavior, I performed behavioral assessments of forelimb motor function at 2 month intervals following induction of adult PTEN deletion. Using the rotarod task to measure baseline coordination and motor learning, and the cylinder task to identify asymmetry in spontaneous forelimb exploration, I found significant improvement in baseline coordination and motor learning beginning at 8 months after PTEN deletion, and spared symmetry of forelimb exploration at all assessed time points. Pulling our structural and functional data together, the timing of functional improvement onset correlates with the progressive development of increased dendritic…

Subjects/Keywords: Neurosciences; Anatomy and Neurobiology

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APA (6^th Edition):

Gallent, E. A. (2018). Approaches to promoting mTOR-mediated growth and regeneration in the mature central nervous system. (Thesis). University of California – Irvine. Retrieved from http://www.escholarship.org/uc/item/98c4k608

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gallent, Erin Ann. “Approaches to promoting mTOR-mediated growth and regeneration in the mature central nervous system.” 2018. Thesis, University of California – Irvine. Accessed December 06, 2019. http://www.escholarship.org/uc/item/98c4k608.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gallent, Erin Ann. “Approaches to promoting mTOR-mediated growth and regeneration in the mature central nervous system.” 2018. Web. 06 Dec 2019.

Vancouver:

Gallent EA. Approaches to promoting mTOR-mediated growth and regeneration in the mature central nervous system. [Internet] [Thesis]. University of California – Irvine; 2018. [cited 2019 Dec 06]. Available from: http://www.escholarship.org/uc/item/98c4k608.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gallent EA. Approaches to promoting mTOR-mediated growth and regeneration in the mature central nervous system. [Thesis]. University of California – Irvine; 2018. Available from: http://www.escholarship.org/uc/item/98c4k608

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – Irvine

2. Karsten, Carley. Mapping brain networks engaged by spaced learning.

Degree: Biomedical Sciences, 2017, University of California – Irvine

URL: http://www.escholarship.org/uc/item/0517c8p3

► Spaced training is a powerful behavioral method for enhancing long-term memory. Despite decades of observational study of this phenomenon, a precise neurobiological mechanism remains unknown.… (more)

▼ Spaced training is a powerful behavioral method for enhancing long-term memory. Despite decades of observational study of this phenomenon, a precise neurobiological mechanism remains unknown. Memory consolidation literature describes both synaptic and systems-level changes that accompany the stabilization of memory traces; the aim of the present work was to determine at which level spaced training operates. A specific spaced training schedule in the object location memory (OLM) task has been shown to dramatically lower the threshold to long-term memory in mice. Here we compare this paradigm to the standard, single-trial, “massed” schedule of training on a number of neurobiological outcomes. First we assayed, across the entire mouse brain, patterns of neuronal activation as reflected by Fos immunoreactivity. This analysis revealed a distinct network difference between spaced and massed training; specifically, the orbitofrontal cortex (OFC) showed significantly different activity. Next we chemogenetically inactivated OFC during massed and spaced training and found that it was necessary for spaced, but not massed, OLM learning. In Chapter 3 we assayed patterns of synaptic modification in hippocampus after massed and spaced OLM training. This analysis did not significantly distinguish between the training schedules. The studies in Chapters 4 and 5 depart from the spaced vs. massed question, and instead venture into territory of intellectual disability. The Fmr1-KO mouse is a commonly-used model of Fragile-X Syndrome, the most common form of inherited intellectual disability in humans. Here we show that these mice fail to exhibit social recognition, and that this deficit is corrected with treatments which enhance TrkB signaling. In Chapter 5, we apply behavioral pattern analysis (Markov sequences) to WT vs. KO exploratory patterns, and then to behavioral tasks discussed in Chapters 1-3 (including spaced vs. massed training and OFC inactivation). The main takeaway of this ethological analysis was that exploratory patterns vary more with environmental novelty than with any other factor. Together the studies in this dissertation support a systems-level effect of spaced training upon long-term memory, which may or may not be related to the difference in exploratory movement patterns.

Subjects/Keywords: Neurosciences; anatomy; hippocampus; memory; spaced training

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Karsten, C. (2017). Mapping brain networks engaged by spaced learning. (Thesis). University of California – Irvine. Retrieved from http://www.escholarship.org/uc/item/0517c8p3

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Karsten, Carley. “Mapping brain networks engaged by spaced learning.” 2017. Thesis, University of California – Irvine. Accessed December 06, 2019. http://www.escholarship.org/uc/item/0517c8p3.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Karsten, Carley. “Mapping brain networks engaged by spaced learning.” 2017. Web. 06 Dec 2019.

Vancouver:

Karsten C. Mapping brain networks engaged by spaced learning. [Internet] [Thesis]. University of California – Irvine; 2017. [cited 2019 Dec 06]. Available from: http://www.escholarship.org/uc/item/0517c8p3.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Karsten C. Mapping brain networks engaged by spaced learning. [Thesis]. University of California – Irvine; 2017. Available from: http://www.escholarship.org/uc/item/0517c8p3

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Stellenbosch University

3. Mazyala, Eric John. A morphological study of the dermal fibroblast.

Degree: MScMedSc (Biomedical Sciences. Anatomy and Histology, Biomedical Sciences, 2008, Stellenbosch University

URL: http://hdl.handle.net/10019.1/4079

Subjects/Keywords: Anatomy and histology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mazyala, E. J. (2008). A morphological study of the dermal fibroblast. (Masters Thesis). Stellenbosch University. Retrieved from http://hdl.handle.net/10019.1/4079

Chicago Manual of Style (16^th Edition):

Mazyala, Eric John. “A morphological study of the dermal fibroblast.” 2008. Masters Thesis, Stellenbosch University. Accessed December 06, 2019. http://hdl.handle.net/10019.1/4079.

MLA Handbook (7^th Edition):

Mazyala, Eric John. “A morphological study of the dermal fibroblast.” 2008. Web. 06 Dec 2019.

Vancouver:

Mazyala EJ. A morphological study of the dermal fibroblast. [Internet] [Masters thesis]. Stellenbosch University; 2008. [cited 2019 Dec 06]. Available from: http://hdl.handle.net/10019.1/4079.

Council of Science Editors:

Mazyala EJ. A morphological study of the dermal fibroblast. [Masters Thesis]. Stellenbosch University; 2008. Available from: http://hdl.handle.net/10019.1/4079

Stellenbosch University

4. Page, Benedict J. (Benedict John). A histological and morphometric assessment of endocrine and ductular proliferation in the adult rat pancreas using an occlusive pancreatic duct ligation model.

Degree: PhD, Biomedical Sciences, 2000, Stellenbosch University

URL: http://hdl.handle.net/10019.1/51573

► ENGLISH ABSTRACT: Diabetes Mellitus (DM) is synonymous with "B-cell failure". Ligation of the pancreatic duct distally to its confluence into the bile duct has been… (more)

▼ ENGLISH ABSTRACT: Diabetes Mellitus (DM) is synonymous with "B-cell failure". Ligation of the pancreatic duct distally to its confluence into the bile duct has been shown to induce endocrine tissue regeneration from a number of probable sources. The cells responsible for regeneration are supposed to possess either dormant pluripotent stem cell ability and/or the plasticity to undergo metaplasia to form new and surplus endocrine tissue able to replace pathologically and/or experimentally compromised pancreas. The sequence of events (cell lineage) during this process of neogenesis, has been the source of controversy for quite some time as various studies suggest that the cell lineage differs from in vivo and in vitro studies, according to experimental model and species of laboratory animal. The object of this study was to utilise an established experimental laboratory animal model to study islet morphological changes, neogenesis and or both in vivo. Further aims of the study were to determine the extent, sequence and magnitude of pancreatic duct ligation (PDL) induced endocrine neogenesis/morphogenesis in a laboratory rat model using occlusive pancreatic duct ligation. PDL's were performed on six groups of 25 normal adult Sprague-Dawley (SD) rats (300g+) according to the method of Hultquist and Jonsson (1965). Experimental animals were sacrificed at 12 hr intervals from day one post-PDL to day 10 and every 24 hrs thereafter to day 14 as described by Wang, Klëppel, Bouwens (1995). Animals received BrdU (a thymidine marker and cell proliferation indicator) 50mglkg intraperitoneally as described by Wang et al. (1995), one hour prior to removal of the pancreas after which it was fixed in Bouin's solution and histologically processed. Seven consecutive 3-6 urn thick serial sections were sequentially stained with H & E, insulin (I), glucagon (G), somatostatin (ST), pancreatic polypeptide (PP), neuropeptide tyrosine (NPY) and peptide tyrosine tyrosine (PYY). Immunolabeling was done according to the method of Guesdon, Temynck , Avrameas (1979). Double immunolabeling for BrdU and each pancreatic peptide was performed on the sections on days 3,5, 7, 9 and 11 as described by Wang et al (1994). Cellular transformation between one and 3Yz days was characterised by simultaneous total deletion and/or transdifferentiation of the acinar compartment and the appearance of immunoreactive cells for I (11.53 ±1.5%), G (1.85 ±0.8%), pp (1.50 ±0.09%), and ST (1.96 ±0.24%). Changes in the endocrine composition in existing islets, occurred along a pathway that saw PP- and ST-cells invading the islet core, islet mantle glucagon deletion and random appearance of all endocrine cell types within the inter-islet interstitium on day 3Yz. Days 4 to 6Yz saw further endocrine expansion while days 7 to 14 were distinguished by islet reconstitution and consolidation. NPY immunoreactivity appeared on day 4Y2 and persisted intermittently throughout while PVV first appeared on day 4 and disappeared after day 7Yz. The… Advisors/Committee Members: Du Toit, Don F., Wolfe-coote, Sonia A., Stellenbosch University. Faculty of Medicine & Health Science. Dept. of Biomedical Sciences..

Subjects/Keywords: Anatomy and histology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Page, B. J. (. J. (2000). A histological and morphometric assessment of endocrine and ductular proliferation in the adult rat pancreas using an occlusive pancreatic duct ligation model. (Doctoral Dissertation). Stellenbosch University. Retrieved from http://hdl.handle.net/10019.1/51573

Chicago Manual of Style (16^th Edition):

Page, Benedict J (Benedict John). “A histological and morphometric assessment of endocrine and ductular proliferation in the adult rat pancreas using an occlusive pancreatic duct ligation model.” 2000. Doctoral Dissertation, Stellenbosch University. Accessed December 06, 2019. http://hdl.handle.net/10019.1/51573.

MLA Handbook (7^th Edition):

Page, Benedict J (Benedict John). “A histological and morphometric assessment of endocrine and ductular proliferation in the adult rat pancreas using an occlusive pancreatic duct ligation model.” 2000. Web. 06 Dec 2019.

Vancouver:

Page BJ(J. A histological and morphometric assessment of endocrine and ductular proliferation in the adult rat pancreas using an occlusive pancreatic duct ligation model. [Internet] [Doctoral dissertation]. Stellenbosch University; 2000. [cited 2019 Dec 06]. Available from: http://hdl.handle.net/10019.1/51573.

Council of Science Editors:

Page BJ(J. A histological and morphometric assessment of endocrine and ductular proliferation in the adult rat pancreas using an occlusive pancreatic duct ligation model. [Doctoral Dissertation]. Stellenbosch University; 2000. Available from: http://hdl.handle.net/10019.1/51573

Stellenbosch University

5. Tchokonte-Nana, Venant. Cellular mechanisms involved in the recapitulation of endocrine development in the duct ligated pancreas.

Degree: PhD, Biomedical Sciences, 2011, Stellenbosch University

URL: http://hdl.handle.net/10019.1/6828

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ENGLISH ABSTRACT: Diabetes mellitus is amongst the leading causes of morbidity and mortality in the world, affecting young, adult and old people. Beta cell replacement… (more)

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ENGLISH ABSTRACT: Diabetes mellitus is amongst the leading causes of morbidity and mortality in the world, affecting young, adult and old people. Beta cell replacement therapy for insulin delivery remains the ultimate remedy for diabetes. However, insufficient donor pancreas and the use of immunosuppressive drugs prevent the wide-spread of this therapy. Other avenues of self generated beta cells within the organ itself need to be explored. Therefore, understanding the chronobiology of cellular mechanisms in the lineage of beta cell induced neogenesis is a valuable tool in improving beta cell replacement in patients with diabetes. The aim of this study was to induce recapitulation of the morpho-genetic sequence of endocrine cells development in the pancreas of rats after the pancreatic duct ligation (PDL) procedure. Serial sections of PDL tissues of the pancreas were obtained from 78 Sprague- Dawley rats and were assessed morphologically. The immunofluorescent tissues were statistically analysed using a computerized morphometry technique. The protein expression indices of Caspase3, Insulin, Pdx1, Ngn3, NeuroD and Pax6 were quantified. The efficiency levels of coexpression of these homeodomain proteins separately with insulin were defined by the ratio of the mean value of insulin expression to the mean value of their respective protein expression. The morphological changes were characterized by the appearance of granulated acinar cells at 6 hours post-PDL and the proliferation of endocrine tissues from 84 hours through to 120 hours. The morpho-immunofluorescent evaluation showed the highest immunoreactivity of Caspase3 and Pdx1 at 6 hours, Ngn3 at 36 hours, Pax6 and insulin at 84 hours while NeuroD expression was at 120 hours. The immunohistofluorescent analysis showed that caspase3 and Pdx1 were the first to be expressed at 6 hours while the insulin and NeuroD expression appeared later at 84 hours and 120 hours, respectively. However, Pax6 expression was continuous across time periods post-PDL, while Ngn3 expression showed a peak at 36 hours. The efficiency (highest and earliest expression) of co-expression of all these homeodomain proteins with insulin was restricted between 12 hours and 24 hours. The optimal efficiency was at 12 hours by Ngn3 with insulin. A good efficiency was shown for Pdx1 with insulin, NeuroD with insulin and Pax6 with insulin at 12 hours and 24 hours, respectively. A low efficiency was observed for insulin and caspase3 co-expression at 24 hours. This study suggests that for transplantation, PDL tissues harvested at an early time post-PDL (between 12 and 24 hours) could yield a higher success rate; the study also provides evidence for a connection between morphological changes in the PDL pancreas and the protein synthesis necessary for the lineage of endocrine cell development.

AFRIKAANSE OPSOMMING: Diabetes Mellitus resorteer onder die vernaamste oorsake van morbiditeit en mortaliteit wêreldwyd, en tuister jongmense, volwassenes en bejaardes. Daar bestaan egter…

Advisors/Committee Members: Page, Benedict J., Du Toit, Don F., University of Stellenbosch. Faculty of Health Sciences. Dept. of Biomedical Sciences. Anatomy and Histology..

Subjects/Keywords: Histology; Dissertations – Anatomy; Dissertations – Histology; Endocrine cell development; Biomedical Sciences. Anatomy and Histology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tchokonte-Nana, V. (2011). Cellular mechanisms involved in the recapitulation of endocrine development in the duct ligated pancreas. (Doctoral Dissertation). Stellenbosch University. Retrieved from http://hdl.handle.net/10019.1/6828

Chicago Manual of Style (16^th Edition):

Tchokonte-Nana, Venant. “Cellular mechanisms involved in the recapitulation of endocrine development in the duct ligated pancreas.” 2011. Doctoral Dissertation, Stellenbosch University. Accessed December 06, 2019. http://hdl.handle.net/10019.1/6828.

MLA Handbook (7^th Edition):

Tchokonte-Nana, Venant. “Cellular mechanisms involved in the recapitulation of endocrine development in the duct ligated pancreas.” 2011. Web. 06 Dec 2019.

Vancouver:

Tchokonte-Nana V. Cellular mechanisms involved in the recapitulation of endocrine development in the duct ligated pancreas. [Internet] [Doctoral dissertation]. Stellenbosch University; 2011. [cited 2019 Dec 06]. Available from: http://hdl.handle.net/10019.1/6828.

Council of Science Editors:

Tchokonte-Nana V. Cellular mechanisms involved in the recapitulation of endocrine development in the duct ligated pancreas. [Doctoral Dissertation]. Stellenbosch University; 2011. Available from: http://hdl.handle.net/10019.1/6828

East Tennessee State University

6. Kim, Jong G. Cytokines and Ovulation in the Mouse Ovary.

Degree: PhD, Biomedical Sciences, 1994, East Tennessee State University

URL: https://dc.etsu.edu/etd/2711

► Ovulation has been hypothesized as an inflammatory process. Interleukin(IL)-1α, IL-1β and tumor necrosis factor(TNF)-α are potent cytokines produced from macrophages and various other cell… (more)

▼ Ovulation has been hypothesized as an inflammatory process. Interleukin(IL)-1α, IL-1β and tumor necrosis factor(TNF)-α are potent cytokines produced from macrophages and various other cell types, and are pivotal components of inflammation. Although previous studies have investigated cytokine activities in the reproductive system, there is little information on their precise localization and activities during the periovulatory period. To investigate the role of cytokines in ovulation, experiments were designed to determine the immunohistochemical localization and time specific production of cytokines IL-1 and TNF-α using a mouse model at 36h, 12h, 6h, 2h before ovulation, and at 6h and 18h after ovulation in vivo. Isolated individual follicles in vitro were used to determine more precise roles of cytokines on follicular development, ovulation and steroidogenesis. From these studies it was found that (1) granulosa cells were the primary sites of IL-1α and TNF-α production from large antral follicles and preovulatory follicles in vivo, (2) production of IL-1α and TNF-α increased as ovulation neared, first appearing in the cumulus cells and expanding to antral and mural granulosa cells, (3) less intense staining of these cytokines in the theca layer of smaller follicles suggests that theca cells may contribute to the production of these cytokines to some extent, (4) but there was no IL-1β production, (5) localized and temporal production of cytokines during the periovulatory period suggests precise regulation, (6) decrease of IL-1α in the ovary after gonadotropin injection determined by enzyme linked immunoadsorbent assay suggests that IL-1α production may be under the control of gonadotropins, (7) in follicle culture without bone marrow derived cells, granulosa cells were confirmed as the main source of cytokine production, (8) addition of IL-1α and TNF-α to follicles in culture tend to decrease estradiol production. In conclusion, immunoreactive cytokine production correlated positively with the periovulatory follicular development suggesting their role as ovulatory mediators. It requires further studies on what are the signals for the initiation and termination of cytokine production, how transcription and translation of these cytokines are regulated during the periovulatory period, and how they contribute to the ovulation.

Subjects/Keywords: Anatomy and physiology; Animals; Biological sciences; Anatomy; Animal Structures

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APA (6^th Edition):

Kim, J. G. (1994). Cytokines and Ovulation in the Mouse Ovary. (Doctoral Dissertation). East Tennessee State University. Retrieved from https://dc.etsu.edu/etd/2711

Chicago Manual of Style (16^th Edition):

Kim, Jong G. “Cytokines and Ovulation in the Mouse Ovary.” 1994. Doctoral Dissertation, East Tennessee State University. Accessed December 06, 2019. https://dc.etsu.edu/etd/2711.

MLA Handbook (7^th Edition):

Kim, Jong G. “Cytokines and Ovulation in the Mouse Ovary.” 1994. Web. 06 Dec 2019.

Vancouver:

Kim JG. Cytokines and Ovulation in the Mouse Ovary. [Internet] [Doctoral dissertation]. East Tennessee State University; 1994. [cited 2019 Dec 06]. Available from: https://dc.etsu.edu/etd/2711.

Council of Science Editors:

Kim JG. Cytokines and Ovulation in the Mouse Ovary. [Doctoral Dissertation]. East Tennessee State University; 1994. Available from: https://dc.etsu.edu/etd/2711

East Tennessee State University

7. Lindsay, Gregory W. Enhanced Renal Sympathetic and Cardiovascular Responses to Substance P in Hypertension.

Degree: PhD, Biomedical Sciences, 1993, East Tennessee State University

URL: https://dc.etsu.edu/etd/2718

► Blood pressure, heart rate, and renal sympathetic nerve responses were measured in 9-13 week old male spontaneously hypertensive rats (SHR) and compared to those… (more)

Subjects/Keywords: Anatomy and physiology; Animals; Biological sciences; Anatomy; Animal Structures

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lindsay, G. W. (1993). Enhanced Renal Sympathetic and Cardiovascular Responses to Substance P in Hypertension. (Doctoral Dissertation). East Tennessee State University. Retrieved from https://dc.etsu.edu/etd/2718

Chicago Manual of Style (16^th Edition):

Lindsay, Gregory W. “Enhanced Renal Sympathetic and Cardiovascular Responses to Substance P in Hypertension.” 1993. Doctoral Dissertation, East Tennessee State University. Accessed December 06, 2019. https://dc.etsu.edu/etd/2718.

MLA Handbook (7^th Edition):

Lindsay, Gregory W. “Enhanced Renal Sympathetic and Cardiovascular Responses to Substance P in Hypertension.” 1993. Web. 06 Dec 2019.

Vancouver:

Lindsay GW. Enhanced Renal Sympathetic and Cardiovascular Responses to Substance P in Hypertension. [Internet] [Doctoral dissertation]. East Tennessee State University; 1993. [cited 2019 Dec 06]. Available from: https://dc.etsu.edu/etd/2718.

Council of Science Editors:

Lindsay GW. Enhanced Renal Sympathetic and Cardiovascular Responses to Substance P in Hypertension. [Doctoral Dissertation]. East Tennessee State University; 1993. Available from: https://dc.etsu.edu/etd/2718

East Tennessee State University

8. Sanders, Theresa A. Quantitation of Teratogenic Effects of 5-fluorouracil Administered to Mice in Vivo or in Submerged Limb Culture.

Degree: PhD, Biomedical Sciences, 1987, East Tennessee State University

URL: https://dc.etsu.edu/etd/2786

► This study demonstrates the use of submerged limb culture in teratologic testing. Pregnant mice were treated on day 11 of gestation (E11, plug date… (more)

▼ This study demonstrates the use of submerged limb culture in teratologic testing. Pregnant mice were treated on day 11 of gestation (E11, plug date = E0) with 10, 20 or 40 mg of 5-fluorouracil (FU) per kg body weight. On E17, treated and untreated fetuses were examined for gross malformations and were fixed in 95% ethanol. Reduction of limb size and digital defects, including ectrodactyly (ED), syndactyly (SD), microdactyly and polydactyly were dose-dependent. In parallel studies, pregnant mice were treated on the morning of E11 and embryos were removed either 7h (E11) or 24h (E12) later for submerged limb culture. Changes in limb area showed a dose-response relationship while treatment had little effect on the shape of individual bones. This indicates the relatively unspecific nature of FU-induced embryotoxicity. E11 studies revealed a dose dependent response of ED, SD and fusion of the metacarpals/metatarsals (MC/MT) to the proximal phalanges. Unlike E11 cultures, middle phalanges were present but decreased in number as dosage increased. Limbs from embryos of untreated females were cultured (E11) in the presence of 0.002, 0.02, 0.2 or 2.0 mg FU/ml culture medium. The percentage of limbs void of paw cartilage or with decreased numbers of MC/MT was dose-dependent. A dose-dependent decrease in the deleterious effects of 0.02 mg FU/ml was observed when 0.2 or 0.02 mg thymine/ml was added to the cultures. In both culture and non-culture studies, hindlimbs (HL) were more affected than forelimbs (FL) and distal regions were more affected than proximal ones. In addition to the morphometric analyses, biochemical parameters of growth and differentiation were examined at 0, 36 and 72h of culture in untreated and treated limbs. Both DNA and protein of FU treated limbs were decreased compared to untreated controls. FL demonstrated greater capacity for regulation of losses in protein content, HL for DNA content. Submerged limb culture provides a useful model for the examination of xenobiotic effects on limb development and allows some comparative evaluation among in vivo, in vivo/in vitro and in vitro studies. (Abstract shortened with permission of author.)

Subjects/Keywords: Anatomy and physiology; Biological sciences; Anatomy; Physiology

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APA (6^th Edition):

Sanders, T. A. (1987). Quantitation of Teratogenic Effects of 5-fluorouracil Administered to Mice in Vivo or in Submerged Limb Culture. (Doctoral Dissertation). East Tennessee State University. Retrieved from https://dc.etsu.edu/etd/2786

Chicago Manual of Style (16^th Edition):

Sanders, Theresa A. “Quantitation of Teratogenic Effects of 5-fluorouracil Administered to Mice in Vivo or in Submerged Limb Culture.” 1987. Doctoral Dissertation, East Tennessee State University. Accessed December 06, 2019. https://dc.etsu.edu/etd/2786.

MLA Handbook (7^th Edition):

Sanders, Theresa A. “Quantitation of Teratogenic Effects of 5-fluorouracil Administered to Mice in Vivo or in Submerged Limb Culture.” 1987. Web. 06 Dec 2019.

Vancouver:

Sanders TA. Quantitation of Teratogenic Effects of 5-fluorouracil Administered to Mice in Vivo or in Submerged Limb Culture. [Internet] [Doctoral dissertation]. East Tennessee State University; 1987. [cited 2019 Dec 06]. Available from: https://dc.etsu.edu/etd/2786.

Council of Science Editors:

Sanders TA. Quantitation of Teratogenic Effects of 5-fluorouracil Administered to Mice in Vivo or in Submerged Limb Culture. [Doctoral Dissertation]. East Tennessee State University; 1987. Available from: https://dc.etsu.edu/etd/2786

Stellenbosch University

9. Mady-Goma, Chancy Rosine. Clinical implications of the variations of sciatic nerve bifurcation on the popliteal nerve block.

Degree: MSc, Biomedical Sciences, 2018, Stellenbosch University

URL: http://hdl.handle.net/10019.1/104922

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ENGLISH ABSTRACT: The sciatic nerve (SN) is a major nerve of the lower limb, innervating the posterior thigh, the hip and knee as well as… (more)

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ENGLISH ABSTRACT: The sciatic nerve (SN) is a major nerve of the lower limb, innervating the posterior thigh, the hip and knee as well as structures below the knee through its branches. The SN division occurs in the popliteal fossa (PF) at the level of the knee. However, various studies report great variations in the level of division of the SN, ranging between 3.3 and 65.1%. These variations were suggested as a possible cause in failures of the popliteal block (PB). Therefore, the aim of this study is to describe the level of division of the SN in a South African cohort and to evaluate the success rate of three approaches to the PB. Following the simulation of the PB in 22 lower limb specimens, the popliteal fossae of 61 cadavers were dissected and the sciatic nerve properly exposed. The level of division was described and the location and distance between the dye and the nerve measured. Variations represented only 11.48% of cases, similar to textbooks’ description (12%). The bifurcation pattern of the SN in this South African cohort was therefore comparable to the standard one. The distance between the SN and the PC varied between 20 mm and 405 mm, with a median of 55 mm, close to 60 – 70 mm found in most studies. The prevalence of variations was higher in females (ratio F:M=2.78) and 55.56% were bilateral. With the SN dividing in the PF, the simulation predicted a 100% success rate with no difference between the approaches used. Nevertheless, a higher division of the SN would compromise the success of the block. Overall, the SN division in our study population follows the normal pattern with a lesser degree of variations (11.48%). The division of the nerve in the PF might ensure a successful block in 95 to 100% of cases, in contrast to cases of high variations. Nevertheless, a preoperative imagery is strongly recommended, especially in women for early identification of variations to avoid failures of the PB, irrespective of the approach used.

AFRIKAANSE OPSOMMING: N. ischiadicus is 'n hoof senuwee in die onderste ledemaat, wat die posterior kompartement van die dy, en die heup en knie voorsien, asook strukture onder die knie deur takke afkomstig vanaf die senuwee. Verdeling van n. ischiadicus vind plaas in die popliteale fossa (PF) op die vlak van die knie. Verskeie studies toon egter ŉ groot aantal variasies, wat wissel tussen 3.3% en 65.1%, ten opsigte van die vlak van verdeling van die senuwee. Daar is voorgestel dat hierdie variasies moontlik die oorsaak van mislukte toediening van ŉ popliteale blok (PB) kan wees. Die doel van hierdie studie is dus om die vlak van verdeling van n. ischiadicus in 'n Suid-Afrikaanse kohort te beskryf, en die voorkoms van sukses ten opsigte van drie benaderings tot die toediening van ŉ PB te evalueer. Na simulasie toediening van ŉ PB in 22 onderste ledemaatmonsters, is die popliteale fossa in 61 kadawers oopgedissekteer en n. ischiadicus blootgelê. Die vlak van verdeling is beskryf, en die ligging van die kleurstof en afstand tussen die kleurstof en die…

Advisors/Committee Members: Tchokonte-Nana, Venant, Ben, Page, Stellenbosch University. Faculty of Health and Medical Sciences. Dept. of Biomedical Sciences. Anatomy and Histology..

Subjects/Keywords: Anatomy; Sciatic nerve; Sciatic nerve – Diseases; Popliteal fossa; UCTD

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mady-Goma, C. R. (2018). Clinical implications of the variations of sciatic nerve bifurcation on the popliteal nerve block. (Masters Thesis). Stellenbosch University. Retrieved from http://hdl.handle.net/10019.1/104922

Chicago Manual of Style (16^th Edition):

Mady-Goma, Chancy Rosine. “Clinical implications of the variations of sciatic nerve bifurcation on the popliteal nerve block.” 2018. Masters Thesis, Stellenbosch University. Accessed December 06, 2019. http://hdl.handle.net/10019.1/104922.

MLA Handbook (7^th Edition):

Mady-Goma, Chancy Rosine. “Clinical implications of the variations of sciatic nerve bifurcation on the popliteal nerve block.” 2018. Web. 06 Dec 2019.

Vancouver:

Mady-Goma CR. Clinical implications of the variations of sciatic nerve bifurcation on the popliteal nerve block. [Internet] [Masters thesis]. Stellenbosch University; 2018. [cited 2019 Dec 06]. Available from: http://hdl.handle.net/10019.1/104922.

Council of Science Editors:

Mady-Goma CR. Clinical implications of the variations of sciatic nerve bifurcation on the popliteal nerve block. [Masters Thesis]. Stellenbosch University; 2018. Available from: http://hdl.handle.net/10019.1/104922

University of California – San Francisco

10. Phan, Vernon T. The Ras-GAP Proteins Ira2 and Neurofibromin Are Negatively Regulated by Ubiquitin-associated Proteins Gpb1 in Yeast and ETEA/UBXD8 in Human Cells.

Degree: Biomedical Sciences, 2008, University of California – San Francisco

URL: http://www.escholarship.org/uc/item/8st7h9w5

► The Neurofibromatosis type 1 (NF1) gene encodes the GTPase activating protein (GAP) neurofibromin which negatively regulates Ras activity. The yeast Saccharomyces cerevisiae has two neurofibromin… (more)

▼ The Neurofibromatosis type 1 (NF1) gene encodes the GTPase activating protein (GAP) neurofibromin which negatively regulates Ras activity. The yeast Saccharomyces cerevisiae has two neurofibromin homologs, Ira1 and Ira2. Similar to mammalian cells, mutations or deletions of the IRA genes result in hyperactive Ras. I utilized an unbiased proteomics approach to investigate Ira2 and neurofibromin binding partners and their involvement in regulating Ras signaling. I demonstrated that the Gpb1 protein negatively regulates Ira2 by promoting Ira2 proteolysis. Loss and gain of function experiments showed the Gpb1 protein is essential for Ira2 function. Whereas deletion of GPB1 increased Ira2 protein levels, overexpression of Gpb1 destabilized Ira2. In addition, the purified Gpb1complex can ubiquitinate Ira2 in vitro. I demonstrated that Gpb1 is required for the Rpn1 proteasome base subunit to trigger Ira2 proteolysis. In addition, I showed that the deubiquitination enzyme Ubp6 interacts with Ira2 and antagonizes Gpb1-mediated degradation of Ira2. Finally, I showed that the serine/threonine kinase CK2 binds and phosphorylates Ira2, preventing Ira2 from protein degradation. I extended the findings to the mammalian system to show that the ETEA/UBXD8 protein directly interacts and negatively regulates neurofibromin. ETEA contains both UBA and UBX domains. Similar to Gpb1 negative regulation of Ira2 in yeast, ETEA over-expression down-regulates neurofibromin in human cells. Purified ETEA, but not a mutant of ETEA that lacks the UBX domain, ubiquitinates the neurofibromin GAP-related domain in vitro. Importantly, ETEA shares approximately 18% homology with Gpb1 N-terminal domain, including amino acid sequence homology in the UBA and UBX domains. Silencing of ETEA increases neurofibromin levels and downregulates Ras activities. These findings provide evidence for conserved ubiquitination pathways regulating the RasGAP proteins Ira2 in yeast and neurofibromin in humans.

Subjects/Keywords: Biology, Anatomy; Health Sciences, Oncology

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APA (6^th Edition):

Phan, V. T. (2008). The Ras-GAP Proteins Ira2 and Neurofibromin Are Negatively Regulated by Ubiquitin-associated Proteins Gpb1 in Yeast and ETEA/UBXD8 in Human Cells. (Thesis). University of California – San Francisco. Retrieved from http://www.escholarship.org/uc/item/8st7h9w5

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Phan, Vernon T. “The Ras-GAP Proteins Ira2 and Neurofibromin Are Negatively Regulated by Ubiquitin-associated Proteins Gpb1 in Yeast and ETEA/UBXD8 in Human Cells.” 2008. Thesis, University of California – San Francisco. Accessed December 06, 2019. http://www.escholarship.org/uc/item/8st7h9w5.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Phan, Vernon T. “The Ras-GAP Proteins Ira2 and Neurofibromin Are Negatively Regulated by Ubiquitin-associated Proteins Gpb1 in Yeast and ETEA/UBXD8 in Human Cells.” 2008. Web. 06 Dec 2019.

Vancouver:

Phan VT. The Ras-GAP Proteins Ira2 and Neurofibromin Are Negatively Regulated by Ubiquitin-associated Proteins Gpb1 in Yeast and ETEA/UBXD8 in Human Cells. [Internet] [Thesis]. University of California – San Francisco; 2008. [cited 2019 Dec 06]. Available from: http://www.escholarship.org/uc/item/8st7h9w5.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Phan VT. The Ras-GAP Proteins Ira2 and Neurofibromin Are Negatively Regulated by Ubiquitin-associated Proteins Gpb1 in Yeast and ETEA/UBXD8 in Human Cells. [Thesis]. University of California – San Francisco; 2008. Available from: http://www.escholarship.org/uc/item/8st7h9w5

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Stellenbosch University

11. Dreyer, Rita Liezl. Anatomical variation of the carotid bifurcation in a Stellenbosch University cadaver cohort.

Degree: MSc, Biomedical Sciences, 2019, Stellenbosch University

URL: http://hdl.handle.net/10019.1/105882

► ENGLISH ABSTRACT: The carotid bifurcation is the point where the common carotid artery bifurcates into the internal and external carotid artery. A precise anatomical knowledge… (more)

▼ ENGLISH ABSTRACT: The carotid bifurcation is the point where the common carotid artery bifurcates into the internal and external carotid artery. A precise anatomical knowledge of the carotid bifurcation is required for various medical specialities. The anatomy of the carotid bifurcation influences the risks, location and prognosis of related pathology. Furthermore, the anatomy of the carotid bifurcation affects treatment as it determines which surgical techniques can be used in an area of high risk. The aim of the study was to determine the anatomical variations of the carotid bifurcation in a Stellenbosch cadaver cohort. One hundred and twenty-eight specimens were examined. This research focuses on the height, angle, general structure, and diameters of the carotid bifurcation, as well as the length and diameter of the carotid sinus. The internal anatomical variation of the carotid bifurcation was added as the study progressed. This study used the gonion as the landmark when measuring height. The Stellenbosch cadaver cohort had a high frequency of high bifurcation with the mean distance of 2.12 cm on the right and 2.06 cm on the left. The angle of bifurcation was 18.53° on the right and 20.24° on the left and was smaller than previous reports in the literature, which ranged between 51-67°. Females had a higher bifurcation and larger angle of bifurcation than males. Sex affected the correlation between angle and height of the bifurcation. The general structure correlated with the standard description and was not influenced by other factors pertaining to the carotid bifurcation, sex or age. Kinks were found in the internal and external carotid artery. The diameters of the carotid bifurcation were larger on the left than on the right. The height of the bifurcation did not influence the probability of kinks in this study, contrary to the literature. The diameters of the internal, external and common carotid arteries in addition to the carotid sinus diameter were larger on the left side and in males. The external carotid had the weakest correlation with the other diameters, which was due to the external carotid artery’s embryological origin. The length of the carotid sinus was 1.74 cm on the right and 1.83 cm on the left. The diameters and the length of the carotid sinus was larger in the males. All external variation slightly increased with age over time as the elasticity of arteries decreased. A variation of the flow diverter was observed in 59% of the cadaver cohort. Supplementary flow diverters were a rare abnormality observed in the internal, external and common carotid arteries. The reason for the carotid bifurcation to present with supplementary flow diverters is still up for debate as this has not been observed in living patients; however, a pathological origin was suggested. Folds in the common carotid were observed. Internal anatomical variation was not affected by external variation or age; however, men had a higher probability of presenting with variation. The Stellenbosch cadaver cohort illustrated… Advisors/Committee Members: Page, Benedict John, Stellenbosch University. Faculty of Medicine and Health Sciences. Dept. of Biomedical Sciences: Anatomy and Histology..

Subjects/Keywords: UCTD; Bifurcation theory; Carotid sinus; Carotid artery; Anatomy – Variation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dreyer, R. L. (2019). Anatomical variation of the carotid bifurcation in a Stellenbosch University cadaver cohort. (Masters Thesis). Stellenbosch University. Retrieved from http://hdl.handle.net/10019.1/105882

Chicago Manual of Style (16^th Edition):

Dreyer, Rita Liezl. “Anatomical variation of the carotid bifurcation in a Stellenbosch University cadaver cohort.” 2019. Masters Thesis, Stellenbosch University. Accessed December 06, 2019. http://hdl.handle.net/10019.1/105882.

MLA Handbook (7^th Edition):

Dreyer, Rita Liezl. “Anatomical variation of the carotid bifurcation in a Stellenbosch University cadaver cohort.” 2019. Web. 06 Dec 2019.

Vancouver:

Dreyer RL. Anatomical variation of the carotid bifurcation in a Stellenbosch University cadaver cohort. [Internet] [Masters thesis]. Stellenbosch University; 2019. [cited 2019 Dec 06]. Available from: http://hdl.handle.net/10019.1/105882.

Council of Science Editors:

Dreyer RL. Anatomical variation of the carotid bifurcation in a Stellenbosch University cadaver cohort. [Masters Thesis]. Stellenbosch University; 2019. Available from: http://hdl.handle.net/10019.1/105882

Stellenbosch University

12. Bergsteedt, Bryan Jason. The sciatic nerve division in the gluteal region in a South African population: An anatomical study.

Degree: MSc, Biomedical Sciences, 2019, Stellenbosch University

URL: http://hdl.handle.net/10019.1/105909

► ENGLISH ABSTRACT: The sciatic nerve is repeatedly involved in the daily medical practices of anaesthesia, neurology, orthopaedics and rehabilitative medicine. The sciatic nerve, and its… (more)

▼ ENGLISH ABSTRACT: The sciatic nerve is repeatedly involved in the daily medical practices of anaesthesia, neurology, orthopaedics and rehabilitative medicine. The sciatic nerve, and its branches, are some of the most frequently injured nerves within the human body. A possible reason for injury could be related to an inadequate knowledge of the anatomical variations of this nerve. Adequate understanding of the anatomical variability within the gluteal region is vital for appropriate diagnosis, potential treatment of gluteal pathology and pain and population-specific anomalies. To the author’s best knowledge, no previous study has described the anatomical variations in relation to the piriformis and sciatic nerve bifurcation within the South African population. Therefore, the aim of the study is to report the prevalence of anatomical variations within the course of the sciatic nerve in relation to the piriformis muscle. Additionally, to report the prevalence of the variations in the level of the sciatic nerve bifurcation. Lastly, to analyse the typical sciatic nerve and piriformis morphomety. The results obtained will be a comparison between sides, sexes, and population groups. For the purpose of this study, lower limbs (𝑁=340) from 170 South African cadavers were selected for dissection and morphological analysis. These specimens consisted of 191 males and 149 females, and comprised of three South African subpopulation groups, namely, White/Caucasian (𝑛=232), Mixed race (𝑛=78) and South African Black (𝑛=30). The variations were recorded, classified and described. Piriformis and sciatic nerve parameters were measured morphometrically using a digital sliding calliper, and statistically analysed. Analysis of the relationship between piriformis and the sciatic nerve resulted in 43 (12.65%) specimens that presented variations in the morphology, while 297 (87.35%) specimens presented normal anatomical features. Variations of these structures occurred predominantly in the South African White/Caucasian population. The bifurcation of the sciatic nerve occurred mainly in the popliteal fossa proper (79.6%). The width of the sciatic nerve was significantly larger in the White/Caucasian group (𝑝<0.05), in comparison to the other two groups. The mean length of the sciatic nerve was significantly larger in the male specimens (𝑝<0.05) in comparison to the female specimens. It was found that the sciatic nerve commonly entered the gluteal region as a single trunk, through the infra-piriform space, inferior to the piriformis muscle. However, variations in the anatomy of the sciatic nerve are common, and are vital in assessing clinical risk, and avoiding debilitating injury or incorrect pain diagnoses. To maintain best possible clinical practices requires regularly updated clinical skills in relation to accurate and relevant new anatomical knowledge. It is for this reason that studies, such as this one, ensure that vital research contributions are available for best clinical practice. Clear uniform landmarks for morphometric analysis… Advisors/Committee Members: Greyling, L. M., Stellenbosch University. Faculty of Medicine and Health Sciences. Dept. of Biomedical Sciences: Anatomy and Histology..

Subjects/Keywords: Sciatic nerve; Piriformis; Morphology; Gluteal region; Bifurcation theory; Anatomy; Variations; UCTD

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bergsteedt, B. J. (2019). The sciatic nerve division in the gluteal region in a South African population: An anatomical study. (Masters Thesis). Stellenbosch University. Retrieved from http://hdl.handle.net/10019.1/105909

Chicago Manual of Style (16^th Edition):

Bergsteedt, Bryan Jason. “The sciatic nerve division in the gluteal region in a South African population: An anatomical study.” 2019. Masters Thesis, Stellenbosch University. Accessed December 06, 2019. http://hdl.handle.net/10019.1/105909.

MLA Handbook (7^th Edition):

Bergsteedt, Bryan Jason. “The sciatic nerve division in the gluteal region in a South African population: An anatomical study.” 2019. Web. 06 Dec 2019.

Vancouver:

Bergsteedt BJ. The sciatic nerve division in the gluteal region in a South African population: An anatomical study. [Internet] [Masters thesis]. Stellenbosch University; 2019. [cited 2019 Dec 06]. Available from: http://hdl.handle.net/10019.1/105909.

Council of Science Editors:

Bergsteedt BJ. The sciatic nerve division in the gluteal region in a South African population: An anatomical study. [Masters Thesis]. Stellenbosch University; 2019. Available from: http://hdl.handle.net/10019.1/105909

Stellenbosch University

13. Alblas, Amanda. Assessment of health status in a 20th century skeletal collection from the Western Cape.

Degree: PhD, Biomedical Sciences, 2019, Stellenbosch University

URL: http://hdl.handle.net/10019.1/106020

► ENGLISH ABSTRACT: Studies on the health status of skeletal remains give insight into the standard of living and survival pattern of historic populations. Analysis of… (more)

▼ ENGLISH ABSTRACT: Studies on the health status of skeletal remains give insight into the standard of living and survival pattern of historic populations. Analysis of trauma and pathological conditions in human skeletal remains are important in biological anthropology, explaining patterns of malnutrition, stress, disease and trauma in a population. However, the difficulty to overcome is the fact that the majority of skeletal pathological conditions are limited in their interpretive significance, since they are nonspecific and a range of stressors can cause the lesions. By analysing multiple conditions within a known population, pathological responses for specific insults can be outlined and in return help in interpretation of the frequencies and distributions within and between populations. The Kirsten Skeletal Collection, housed at Stellenbosch University, Division of Clinical Anatomy broadly represents individuals from mainly low socio-economic communities of different population groups in the Western Cape, dating throughout the 20th century. The aim of this study was to macroscopically and radiologically examine adult individuals (n=624, nmales=438, nfemales=186) in this collection for skeletal markings that included malnutrition (diet and metabolic deficiencies), osteoarthritic lesions, neoplasms, infective diseases and antemortem trauma lesions to be used as baseline information in further anthropological studies on the people of the Western Cape region. Statistically, the prevalence of specific diseases or trauma were correlated between the sexes, three different age-at-death and population groups as well as three different time periods throughout the 20th century using two-way frequency-tests and correspondence analyses. During the 20th century, many factors resulted in poverty, and "non white” people, namely the South African Black (SAB) and South African Coloured (SAC) population groups, was especially disadvantaged by the laws introduced by the ruling political party. The influx of people from rural areas during World War II to work in the manufacturing industry resulted in the already overcrowded, unsanitary informal settlements around the Cape Peninsula to be flooded, influencing the disease patterns in the communities. The results demonstrated that the lowest prevalence of metabolic deficiencies, iron deficiency anaemia (porotic hyperostosis), growth arrest signs (Harris’ lines), infections such as tuberculosis, osteomyelitis and non-specific periosteal reactions were observed in the South African White (SAW) population group. This confirms that higher socio economic societies, that escaped the unsanitary conditions associated with poor housing and overcrowding environments, were more succesfully buffering themselves from malnutrition and exposure to pathogens. Better dental health as well as dental fillings were also more associated with the SAW population group that had unrestrained access to dentists and health care facilities. In contrast, the ‘non-white’ population groups, that were supressed… Advisors/Committee Members: Friedling, Louise Jacqui, Greyling, Linda Magdalena, Stellenbosch University. Faculty of Medicine and Health Science. Dept. of Biomedical Sciences: Anatomy and Histology..

Subjects/Keywords: Kirsten Skeletal Collection; Human skeleton – Abnormalities; Anatomy, Pathological

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Alblas, A. (2019). Assessment of health status in a 20th century skeletal collection from the Western Cape. (Doctoral Dissertation). Stellenbosch University. Retrieved from http://hdl.handle.net/10019.1/106020

Chicago Manual of Style (16^th Edition):

Alblas, Amanda. “Assessment of health status in a 20th century skeletal collection from the Western Cape.” 2019. Doctoral Dissertation, Stellenbosch University. Accessed December 06, 2019. http://hdl.handle.net/10019.1/106020.

MLA Handbook (7^th Edition):

Alblas, Amanda. “Assessment of health status in a 20th century skeletal collection from the Western Cape.” 2019. Web. 06 Dec 2019.

Vancouver:

Alblas A. Assessment of health status in a 20th century skeletal collection from the Western Cape. [Internet] [Doctoral dissertation]. Stellenbosch University; 2019. [cited 2019 Dec 06]. Available from: http://hdl.handle.net/10019.1/106020.

Council of Science Editors:

Alblas A. Assessment of health status in a 20th century skeletal collection from the Western Cape. [Doctoral Dissertation]. Stellenbosch University; 2019. Available from: http://hdl.handle.net/10019.1/106020

East Tennessee State University

14. Levee, Kathryn E. Gross and Histological Features of a Myofascial Trigger Point in the Upper Trapezius.

Degree: PhD, Biomedical Sciences, 1996, East Tennessee State University

URL: https://dc.etsu.edu/etd/2938

► The purpose of this study was to precisely locate, in living humans, a myofascial trigger point associated with the upper portion of the trapezius… (more)

Subjects/Keywords: Anatomy and physiology; Biological sciences; Headache; Neurology; Pain; Anatomy; Neurology; Physiology

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APA (6^th Edition):

Levee, K. E. (1996). Gross and Histological Features of a Myofascial Trigger Point in the Upper Trapezius. (Doctoral Dissertation). East Tennessee State University. Retrieved from https://dc.etsu.edu/etd/2938

Chicago Manual of Style (16^th Edition):

Levee, Kathryn E. “Gross and Histological Features of a Myofascial Trigger Point in the Upper Trapezius.” 1996. Doctoral Dissertation, East Tennessee State University. Accessed December 06, 2019. https://dc.etsu.edu/etd/2938.

MLA Handbook (7^th Edition):

Levee, Kathryn E. “Gross and Histological Features of a Myofascial Trigger Point in the Upper Trapezius.” 1996. Web. 06 Dec 2019.

Vancouver:

Levee KE. Gross and Histological Features of a Myofascial Trigger Point in the Upper Trapezius. [Internet] [Doctoral dissertation]. East Tennessee State University; 1996. [cited 2019 Dec 06]. Available from: https://dc.etsu.edu/etd/2938.

Council of Science Editors:

Levee KE. Gross and Histological Features of a Myofascial Trigger Point in the Upper Trapezius. [Doctoral Dissertation]. East Tennessee State University; 1996. Available from: https://dc.etsu.edu/etd/2938

Stellenbosch University

15. Nel, Simoné. Histomorphometric effects of an antiretroviral treatment and obesity on the pancreas, liver, kidney and perivascular adipose tissue in a rat model.

Degree: MSc, Biomedical Sciences, 2017, Stellenbosch University

URL: http://hdl.handle.net/10019.1/102974

► ENGLISH ABSTRACT : Human immunodeficiency virus (HIV)-infected patients receiving antiretroviral treatment (ART) may develop metabolic syndrome and lipodystrophy, leading to obesity rather than wasting. Obesity… (more)

▼ ENGLISH ABSTRACT : Human immunodeficiency virus (HIV)-infected patients receiving antiretroviral treatment (ART) may develop metabolic syndrome and lipodystrophy, leading to obesity rather than wasting. Obesity is associated with metabolic homeostasis disruptions leading to pancreatic toxicity, diabetes, renal vasodilation and compression as well as liver steatosis. In addition, patients on long-term combination antiretroviral treatment (cART) can develop pancreatitis, nephrotoxicity, hepatic steatosis, and diabetes. This study aims to investigate the effects of cART combined with a high-calorie diet (HCD) on the histomorphometry of the pancreas, kidney, liver, and aortic perivascular adipose tissue (PVAT) leptin expression in the rat. Male Wistar rats (N=40) were divided into four groups (n=10); C/ART- (lean control), C/ART+ (ART control), HCD/ART- (HCD control) and HCD/ART+ (HCD and ART). The aorta with surrounding PVAT, pancreas, liver and kidney were harvested and processed to wax. Pancreas sections underwent a double immunohistochemistry (IHC) labelling for insulin and glucagon and reactive cell areas were measured. Aortic PVAT was labelled with anti-leptin and the staining intensity classified accordingly. Kidney sections underwent haematoxylin and eosin (H&E) stain measuring the areas of the renal corpuscles, glomeruli, renal spaces as well as the renal arcuate arteries lumen diameter and artery wall thickness. Pancreatic, kidney and liver sections underwent H&E staining which were used for pathology assessment. The HCD/ART+ had a significant body mass increase compared to the C/ART-, suggesting ART combined with a HCD causes body mass increase. Unilocular and differentiating adipocytes in the C/ART+ had intense leptin staining, suggesting ART may lead to increased leptin expression in aortic PVAT. Unilocular and differentiating adipocytes in the HCD/ART- and HCD/ART+ had weak to no leptin intensity, suggesting possible leptin deficiency with a HCD. The C/ART+ showed a trend in smaller total pancreatic islet area compared to the C/ART-, suggesting that islets underwent possible hypotrophy with ART. Based on the average values, alpha-cells were increased in the HCD/ART-, whereas beta-cells decreased in the ART and HCD. This suggests that a HCD potentially increases glucagon secreting area, whereas ART and a HCD leads to β-cell mass loss. The HCD/ART- and HCD/ART+ showed a trend in increased number of islets per section compared to the C/ART-, suggesting that HCD leads to new forming islets. Based on the average values, the renal corpuscle, glomeruli and renal space sizes were increased in the HCD/ART- compared to the other groups, although not significantly. A trend in the HCD/ART+ showed increased arcuate artery lumen diameter and tunica media thickness, whereas the C/ART+ had increased tunica adventitia compared to the C/ART-. This suggests that ART combined with a HCD causes hypertrophic remodelling in the tunica media and hypotrophic remodelling in the tunica adventitia. All groups had mild vacuolisation… Advisors/Committee Members: Kotzé, Sanet Henriette, Nireshni, Chellan, Stellenbosch University. Faculty of Medicine and Health Sciences. Dept. of Biomedical Sciences. Division Anatomy and Histology..

Subjects/Keywords: Immunohistochemistry; UCTD; Rats – Antiretroviral treatment; Rats as laboratory animals; Obesity – Animal models; Organs (Anatomy) – Side effects

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APA (6^th Edition):

Nel, S. (2017). Histomorphometric effects of an antiretroviral treatment and obesity on the pancreas, liver, kidney and perivascular adipose tissue in a rat model. (Masters Thesis). Stellenbosch University. Retrieved from http://hdl.handle.net/10019.1/102974

Chicago Manual of Style (16^th Edition):

Nel, Simoné. “Histomorphometric effects of an antiretroviral treatment and obesity on the pancreas, liver, kidney and perivascular adipose tissue in a rat model.” 2017. Masters Thesis, Stellenbosch University. Accessed December 06, 2019. http://hdl.handle.net/10019.1/102974.

MLA Handbook (7^th Edition):

Nel, Simoné. “Histomorphometric effects of an antiretroviral treatment and obesity on the pancreas, liver, kidney and perivascular adipose tissue in a rat model.” 2017. Web. 06 Dec 2019.

Vancouver:

Nel S. Histomorphometric effects of an antiretroviral treatment and obesity on the pancreas, liver, kidney and perivascular adipose tissue in a rat model. [Internet] [Masters thesis]. Stellenbosch University; 2017. [cited 2019 Dec 06]. Available from: http://hdl.handle.net/10019.1/102974.

Council of Science Editors:

Nel S. Histomorphometric effects of an antiretroviral treatment and obesity on the pancreas, liver, kidney and perivascular adipose tissue in a rat model. [Masters Thesis]. Stellenbosch University; 2017. Available from: http://hdl.handle.net/10019.1/102974

Stellenbosch University

16. Witbooi, Lee-Roy Cecil. Accurate estimation of large vessel length in growing children and adolescents for the purpose of pulse wave velocity calculation.

Degree: MMed, Biomedical Sciences, 2018, Stellenbosch University

URL: http://hdl.handle.net/10019.1/105087

► Background Cardiovascular disease is a major cause of death in adults worldwide. Early detection allows for early intervention to prevent vascular events such as strokes,… (more)

▼ Background Cardiovascular disease is a major cause of death in adults worldwide. Early detection allows for early intervention to prevent vascular events such as strokes, heart attacks, etc. Although these vascular events typically occur in late adulthood, the underlying atherosclerosis often begins during childhood. Early subclinical atherosclerosis can be detected by measuring the elasticity of the large arteries, particularly when performed serially over time. Normally, the elasticity of a healthy aorta helps to slow down the speed of the pressure wave created by contraction of the heart muscle. This is an important way of maintaining smooth laminar blood flow. Atherosclerosis causes the vessel wall to harden and lose elasticity. As the vessel wall hardens, the speed of the pressure wave increases. Pulse wave velocity (PWV) is a sophisticated method of detecting early elasticity changes, and is a preferred non-invasive technique to measure arterial wall stiffness. The velocity calculation requires accurate measurement of both distance travelled and time taken for the pulse wave to travel between two points. The distance used for pulse wave velocity calculation is an approximation of the intraluminal distance travelled by the pulse wave and is estimated by measuring the distance between various surface anatomy landmarks. The expert consensus document on arterial wall stiffness described carotid–femoral PWV as the “gold standard” measurement of arterial wall stiffness, yet there is no consensus on the arterial path length estimation method. A variety of arterial path length estimation methods exist, and this makes inter-study comparison of PWV very difficult. The purpose of the current study was to investigate the most accurate method of estimating the true distance travelled by the aorto-femoral pressure wave. We compared distances between a range of commonly used surface anatomy landmarks, and compared these to the true intraluminal distance measured on multi-planar reformations of archived computerized tomography imaging in children of varying ages. Our findings will allow standardization of PWV calculation in children and allow for inter-study comparisons. Methods Vessel lengths in children (aged 0-18 years) were measured with multi-planar reformation (MPR) imaging software. These measurements were then compared with the surface anatomy measurements also obtained using the MPR imaging software. The comparisons between vessel lengths and surface anatomy distances were performed in segments, since there were no whole body CT scans available on the Picture Archiving and Communication System (PACS) at the research site. Results The surface anatomy measurements from the suprasternal notch to the angle of the mandible (on the right) correlated well with the intraluminal vessel length from the origin of the brachiocephalic trunk to the external carotid at the angle of the mandible (r2=0.92; p<0.0001). The surface anatomy measurements from the suprasternal notch to the midpoint of the right… Advisors/Committee Members: Innes, Steve, Page, Ben, Pitcher, Richard, Stellenbosch University. Faculty of Medicine and Health Sciences. Dept. of Biomedical Sciences. Anatomy and Histology..

Subjects/Keywords: Vascular diseases; Cardiovascular diseases; Atherosclerosis; Children; Adolescents; Surface anatomy; Pulse wave velocity; Multidetector-row helical computed tomography; Body section radiography; UCTD

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APA (6^th Edition):

Witbooi, L. C. (2018). Accurate estimation of large vessel length in growing children and adolescents for the purpose of pulse wave velocity calculation. (Thesis). Stellenbosch University. Retrieved from http://hdl.handle.net/10019.1/105087

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Witbooi, Lee-Roy Cecil. “Accurate estimation of large vessel length in growing children and adolescents for the purpose of pulse wave velocity calculation.” 2018. Thesis, Stellenbosch University. Accessed December 06, 2019. http://hdl.handle.net/10019.1/105087.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Witbooi, Lee-Roy Cecil. “Accurate estimation of large vessel length in growing children and adolescents for the purpose of pulse wave velocity calculation.” 2018. Web. 06 Dec 2019.

Vancouver:

Witbooi LC. Accurate estimation of large vessel length in growing children and adolescents for the purpose of pulse wave velocity calculation. [Internet] [Thesis]. Stellenbosch University; 2018. [cited 2019 Dec 06]. Available from: http://hdl.handle.net/10019.1/105087.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Witbooi LC. Accurate estimation of large vessel length in growing children and adolescents for the purpose of pulse wave velocity calculation. [Thesis]. Stellenbosch University; 2018. Available from: http://hdl.handle.net/10019.1/105087

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

17. Lafferty, Dennis Craig. The mGluR2/3 Agonist LY397268 Improves Morphometric and Behavioral Outcomes in R6/2 Huntington's Disease Mice.

Degree: MS, Biomedical Sciences, 2010, University of Tennessee Health Science Center

URL: https://dc.uthsc.edu/dissertations/152

► A prominent theory for the pathology of Huntington's Disease (HD) is the excitotoxic injury to the striatum. Continual exposure of ionotropic NMDA receptors to… (more)

Subjects/Keywords: Basal Ganglia; Huntington’s Disease; Diseases; Medical Anatomy; Medical Sciences; Medicine and Health Sciences; Nervous System Diseases; Neurosciences

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APA (6^th Edition):

Lafferty, D. C. (2010). The mGluR2/3 Agonist LY397268 Improves Morphometric and Behavioral Outcomes in R6/2 Huntington's Disease Mice. (Thesis). University of Tennessee Health Science Center. Retrieved from https://dc.uthsc.edu/dissertations/152

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lafferty, Dennis Craig. “The mGluR2/3 Agonist LY397268 Improves Morphometric and Behavioral Outcomes in R6/2 Huntington's Disease Mice.” 2010. Thesis, University of Tennessee Health Science Center. Accessed December 06, 2019. https://dc.uthsc.edu/dissertations/152.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lafferty, Dennis Craig. “The mGluR2/3 Agonist LY397268 Improves Morphometric and Behavioral Outcomes in R6/2 Huntington's Disease Mice.” 2010. Web. 06 Dec 2019.

Vancouver:

Lafferty DC. The mGluR2/3 Agonist LY397268 Improves Morphometric and Behavioral Outcomes in R6/2 Huntington's Disease Mice. [Internet] [Thesis]. University of Tennessee Health Science Center; 2010. [cited 2019 Dec 06]. Available from: https://dc.uthsc.edu/dissertations/152.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lafferty DC. The mGluR2/3 Agonist LY397268 Improves Morphometric and Behavioral Outcomes in R6/2 Huntington's Disease Mice. [Thesis]. University of Tennessee Health Science Center; 2010. Available from: https://dc.uthsc.edu/dissertations/152

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

18. Narayanan, Damodaran. Mitochondrial Regulation of Arterial Contractility.

Degree: PhD, Biomedical Sciences, 2010, University of Tennessee Health Science Center

URL: https://dc.uthsc.edu/dissertations/177

► Rationale: Physiological functions of mitochondria in contractile arterial smooth muscle cells are poorly understood. Mitochondria can uptake calcium (Ca²⁺), but intracellular Ca²⁺ signals that… (more)

Subjects/Keywords: arterial smooth muscle; calcium signaling; CaV1.2 expression; mitochondria; myogenic tone; Anatomy; Cardiovascular System; Medicine and Health Sciences

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APA (6^th Edition):

Narayanan, D. (2010). Mitochondrial Regulation of Arterial Contractility. (Doctoral Dissertation). University of Tennessee Health Science Center. Retrieved from https://dc.uthsc.edu/dissertations/177

Chicago Manual of Style (16^th Edition):

Narayanan, Damodaran. “Mitochondrial Regulation of Arterial Contractility.” 2010. Doctoral Dissertation, University of Tennessee Health Science Center. Accessed December 06, 2019. https://dc.uthsc.edu/dissertations/177.

MLA Handbook (7^th Edition):

Narayanan, Damodaran. “Mitochondrial Regulation of Arterial Contractility.” 2010. Web. 06 Dec 2019.

Vancouver:

Narayanan D. Mitochondrial Regulation of Arterial Contractility. [Internet] [Doctoral dissertation]. University of Tennessee Health Science Center; 2010. [cited 2019 Dec 06]. Available from: https://dc.uthsc.edu/dissertations/177.

Council of Science Editors:

Narayanan D. Mitochondrial Regulation of Arterial Contractility. [Doctoral Dissertation]. University of Tennessee Health Science Center; 2010. Available from: https://dc.uthsc.edu/dissertations/177

East Tennessee State University

19. Abraham, Sonny T. Denervation Supersensitivity of the Rat Vas Deferens: A Role for Protein Kinase C.

Degree: PhD, Biomedical Sciences, 1994, East Tennessee State University

URL: https://dc.etsu.edu/etd/2622

► A role for protein kinase C (PKC) in the denervation-induced supersensitivity of the rat vas deferens was investigated. Chronic, surgical denervation of the rat… (more)

▼ A role for protein kinase C (PKC) in the denervation-induced supersensitivity of the rat vas deferens was investigated. Chronic, surgical denervation of the rat vas deferens (up to 8 days) resulted in tissues that produced enhanced contractile responses to norepinephrine (NE) in isolated organ baths. Single challenges of NE (10 μM) produced 0.6 ± 0.1 g of maximal tension in the control vas whereas in the paired, denervated tissue 2.2 ± 0.3 g of tension was recorded (n = 6). Cumulative concentration-effect curves to NE produced in the denervated vas deferens were shifted 18-fold to the left of the control response. Neurokinin A (NKA) responses after denervation of the tissue were not significantly different from the control. Denervation did not alter the contractile response to phorbol diacetate (PDA), a PKC activator. Pretreatment of denervated and control vas deferens with 100 μM nifedipine (a calcium channel blocker), significantly attenuated the contractile response to NE. The responses in the control tissues were depressed by 88%, those in the denervated vasa were only antagonized by 65% after nifedipine treatment. Exposure of denervated and control vas deferens to 100 μM NE resulted in no significant accumulation of diacylglycerol (DAG) from basal values. The molecular species of DAG produced after receptor stimulation, in either tissue group, were not different from those found in resting tissues. Denervation also had no effect on the binding characteristics of membrane-associated PKC when assayed using the specific ligand, (³H) phorbol dibutyrate. The PKC activity of resting vas deferens was not altered by chronic surgical denervation. Denervated and control vas deferens that were stimulated with 100 μM NE showed a time-dependent translocation of PKC from the cytosolic to the membrane fraction of the tissue. In both tissue groups exposure to NE resulted in a 3-4 fold increase in the membrane-bound PKC activity, which remained elevated above basal values for up to 20 min. The rate of translocation of PKC was faster in denervated vasa (maximal at 5 min after NE) when compared to the control (maximal at 20 min), but the maximal amount of the enzyme activated was the same for the two tissue groups. The ability of NKA, 60 mM K⁺-depolarization and PDB (PKC activator) to produce translocation of the PKC was not altered by denervation of the vas deferens. (Abstract shortened by UMI.)

Subjects/Keywords: Anatomy and physiology; Animals; Biological sciences; Health and environmental sciences; Pharmacology; Anatomy; Animal Structures; Pharmacology

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APA (6^th Edition):

Abraham, S. T. (1994). Denervation Supersensitivity of the Rat Vas Deferens: A Role for Protein Kinase C. (Doctoral Dissertation). East Tennessee State University. Retrieved from https://dc.etsu.edu/etd/2622

Chicago Manual of Style (16^th Edition):

Abraham, Sonny T. “Denervation Supersensitivity of the Rat Vas Deferens: A Role for Protein Kinase C.” 1994. Doctoral Dissertation, East Tennessee State University. Accessed December 06, 2019. https://dc.etsu.edu/etd/2622.

MLA Handbook (7^th Edition):

Abraham, Sonny T. “Denervation Supersensitivity of the Rat Vas Deferens: A Role for Protein Kinase C.” 1994. Web. 06 Dec 2019.

Vancouver:

Abraham ST. Denervation Supersensitivity of the Rat Vas Deferens: A Role for Protein Kinase C. [Internet] [Doctoral dissertation]. East Tennessee State University; 1994. [cited 2019 Dec 06]. Available from: https://dc.etsu.edu/etd/2622.

Council of Science Editors:

Abraham ST. Denervation Supersensitivity of the Rat Vas Deferens: A Role for Protein Kinase C. [Doctoral Dissertation]. East Tennessee State University; 1994. Available from: https://dc.etsu.edu/etd/2622

East Tennessee State University

20. Khalbuss, Walid E. Electrophysiology, Cell Calcium, and Mechanisms of Hepatocyte Volume Regulation.

Degree: PhD, Biomedical Sciences, 1990, East Tennessee State University

URL: https://dc.etsu.edu/etd/2709

► The electrophysiologic technique (Reuss, L., Proc. Natl. Acad. Sci. USA 82:6014, 1985) was modified to measure changes in steady-state hepatocyte volume during osmotic stress.… (more)

Subjects/Keywords: Anatomy and physiology; Animals; Biological sciences; Biology; Calcium; Cellular biology; Anatomy; Animal Structures; Biology; Cell Biology

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APA (6^th Edition):

Khalbuss, W. E. (1990). Electrophysiology, Cell Calcium, and Mechanisms of Hepatocyte Volume Regulation. (Doctoral Dissertation). East Tennessee State University. Retrieved from https://dc.etsu.edu/etd/2709

Chicago Manual of Style (16^th Edition):

Khalbuss, Walid E. “Electrophysiology, Cell Calcium, and Mechanisms of Hepatocyte Volume Regulation.” 1990. Doctoral Dissertation, East Tennessee State University. Accessed December 06, 2019. https://dc.etsu.edu/etd/2709.

MLA Handbook (7^th Edition):

Khalbuss, Walid E. “Electrophysiology, Cell Calcium, and Mechanisms of Hepatocyte Volume Regulation.” 1990. Web. 06 Dec 2019.

Vancouver:

Khalbuss WE. Electrophysiology, Cell Calcium, and Mechanisms of Hepatocyte Volume Regulation. [Internet] [Doctoral dissertation]. East Tennessee State University; 1990. [cited 2019 Dec 06]. Available from: https://dc.etsu.edu/etd/2709.

Council of Science Editors:

Khalbuss WE. Electrophysiology, Cell Calcium, and Mechanisms of Hepatocyte Volume Regulation. [Doctoral Dissertation]. East Tennessee State University; 1990. Available from: https://dc.etsu.edu/etd/2709

East Tennessee State University

21. Miao, Kai. Microcirculation: Electrophysiological Basis for the Response of Endothelial Cells to Inflammatory Mediators-bradykinin.

Degree: PhD, Biomedical Sciences, 1994, East Tennessee State University

URL: https://dc.etsu.edu/etd/2749

► Using conventional microelectrodes, I studied the electrical basis for determining the resting V_m in intact EC's from hamsters. The resting V_m were… (more)

Subjects/Keywords: Anatomy and physiology; Animals; Biological sciences; Bradykinin; Cellular biology; Anatomy; Animal Structures; Cell Biology

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APA (6^th Edition):

Miao, K. (1994). Microcirculation: Electrophysiological Basis for the Response of Endothelial Cells to Inflammatory Mediators-bradykinin. (Doctoral Dissertation). East Tennessee State University. Retrieved from https://dc.etsu.edu/etd/2749

Chicago Manual of Style (16^th Edition):

Miao, Kai. “Microcirculation: Electrophysiological Basis for the Response of Endothelial Cells to Inflammatory Mediators-bradykinin.” 1994. Doctoral Dissertation, East Tennessee State University. Accessed December 06, 2019. https://dc.etsu.edu/etd/2749.

MLA Handbook (7^th Edition):

Miao, Kai. “Microcirculation: Electrophysiological Basis for the Response of Endothelial Cells to Inflammatory Mediators-bradykinin.” 1994. Web. 06 Dec 2019.

Vancouver:

Miao K. Microcirculation: Electrophysiological Basis for the Response of Endothelial Cells to Inflammatory Mediators-bradykinin. [Internet] [Doctoral dissertation]. East Tennessee State University; 1994. [cited 2019 Dec 06]. Available from: https://dc.etsu.edu/etd/2749.

Council of Science Editors:

Miao K. Microcirculation: Electrophysiological Basis for the Response of Endothelial Cells to Inflammatory Mediators-bradykinin. [Doctoral Dissertation]. East Tennessee State University; 1994. Available from: https://dc.etsu.edu/etd/2749

East Tennessee State University

22. Tang, Tao. Differential Role of the Endothelium in Regulating Microvascular Blood Flow.

Degree: PhD, Biomedical Sciences, 1994, East Tennessee State University

URL: https://dc.etsu.edu/etd/2802

► The vascular endothelial cell (EC) plays an important role in regulating vascular tone and local blood flow by sensing chemical and mechanical stimuli on… (more)

▼ The vascular endothelial cell (EC) plays an important role in regulating vascular tone and local blood flow by sensing chemical and mechanical stimuli on the vascular wall and releasing a host of vasoactive substances upon activations of endogenous or exogenous vasoactive substances. The central hypothesis is that local control of blood now and autoregulatory behavior in the microcirculation is distinctive at different levels of the vasculature and is dependent on the cellular activities of the EC and its interaction with the local environment. The in vivo as well as the ex vivo, flow-controlled preparations of the hamster cheek pouch were utilized. Inhibition of Endothelium-Derive Relaxing Factor (EDRF) synthesis and the functional impairment by light-dye (L-D) treatment were used to remove functional characteristics of the EC. It is found that the EC played differential roles in modulating vascular tone and blood flow in distinct segments of arterioles. Impairment of the EC by L-D treatment significantly reduced both acetylcholine (Ach)-induced dilation and the local angiotensin conversion in small (4th order) arterioles (A₄). Whereas, data obtained after inhibition of EDRF synthesis indicated that EDRF pathway appeared to be the dominant regulatory mechanism mediating agonists (e.g. Ach)-induced responses in these small vessels. In large (2nd order) arterioles (A₂), on the other hand, neither L-D treatment nor EDRF inhibition affected Ach-induced dilation or local angiotensin conversion; therefore, these responses seemed to be independent of the EC or EDRF pathway. Autoregulation was observed in both A₂ and A₄ when perfusion flow (shear stress) and perfusion pressure (stretch) were elevated. Nevertheless, the underlying regulatory mechanisms in response to mechanical stimuli differed in these series-arranged arterioles. The EC/EDRF-dependent, flow-induced dilation was dominant in A₂; whereas, the myogenic autoregulation (which appears to be independent of the EC) played major role in A₄. Therefore, the function of the EC does not appear homogenous throughout the arteriolar portion of the microcirculation. Thus, the local control of blood flow and autoregulatory behavior in the microcirculation is distinctive at different levels of the vasculature; whereas, the differential role of the EC in discrete segments of series-arranged arterioles seems to be the determinant for these differences. These differential modulations of vascular tone and blood flow by the EC at discrete levels of the microcirculation may have important implications in pathological conditions, such as hypertension, diabetes, and atherosclerosis.

Subjects/Keywords: Anatomy and physiology; Animals; Biological sciences; Health and environmental sciences; Pharmacology; Anatomy; Animal Structures; Pharmacology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tang, T. (1994). Differential Role of the Endothelium in Regulating Microvascular Blood Flow. (Doctoral Dissertation). East Tennessee State University. Retrieved from https://dc.etsu.edu/etd/2802

Chicago Manual of Style (16^th Edition):

Tang, Tao. “Differential Role of the Endothelium in Regulating Microvascular Blood Flow.” 1994. Doctoral Dissertation, East Tennessee State University. Accessed December 06, 2019. https://dc.etsu.edu/etd/2802.

MLA Handbook (7^th Edition):

Tang, Tao. “Differential Role of the Endothelium in Regulating Microvascular Blood Flow.” 1994. Web. 06 Dec 2019.

Vancouver:

Tang T. Differential Role of the Endothelium in Regulating Microvascular Blood Flow. [Internet] [Doctoral dissertation]. East Tennessee State University; 1994. [cited 2019 Dec 06]. Available from: https://dc.etsu.edu/etd/2802.

Council of Science Editors:

Tang T. Differential Role of the Endothelium in Regulating Microvascular Blood Flow. [Doctoral Dissertation]. East Tennessee State University; 1994. Available from: https://dc.etsu.edu/etd/2802

East Tennessee State University

23. Wang, Kening. Changes in Intracellular Chloride During Osmotic Stress and L-alanine Uptake in Mouse Hepatocytes.

Degree: PhD, Biomedical Sciences, 1992, East Tennessee State University

URL: https://dc.etsu.edu/etd/2821

► A stable intracellular ionic environment is necessary for hepatocytes to function normally. Thus, during hypotonic shock or L-alanine uptake, hepatocytes swell and then exhibit… (more)

Subjects/Keywords: Alanine uptake; Anatomy and physiology; Animals; Biological sciences; Cellular biology; Anatomy; Animal Structures; Cell Biology

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APA (6^th Edition):

Wang, K. (1992). Changes in Intracellular Chloride During Osmotic Stress and L-alanine Uptake in Mouse Hepatocytes. (Doctoral Dissertation). East Tennessee State University. Retrieved from https://dc.etsu.edu/etd/2821

Chicago Manual of Style (16^th Edition):

Wang, Kening. “Changes in Intracellular Chloride During Osmotic Stress and L-alanine Uptake in Mouse Hepatocytes.” 1992. Doctoral Dissertation, East Tennessee State University. Accessed December 06, 2019. https://dc.etsu.edu/etd/2821.

MLA Handbook (7^th Edition):

Wang, Kening. “Changes in Intracellular Chloride During Osmotic Stress and L-alanine Uptake in Mouse Hepatocytes.” 1992. Web. 06 Dec 2019.

Vancouver:

Wang K. Changes in Intracellular Chloride During Osmotic Stress and L-alanine Uptake in Mouse Hepatocytes. [Internet] [Doctoral dissertation]. East Tennessee State University; 1992. [cited 2019 Dec 06]. Available from: https://dc.etsu.edu/etd/2821.

Council of Science Editors:

Wang K. Changes in Intracellular Chloride During Osmotic Stress and L-alanine Uptake in Mouse Hepatocytes. [Doctoral Dissertation]. East Tennessee State University; 1992. Available from: https://dc.etsu.edu/etd/2821

East Tennessee State University

24. Chen, Yuejin. Characterization of the Vasoactivity of Tachykinins in Isolated Rat Kidney: Functional Studies and in Vitro Receptor Autoradiography.

Degree: PhD, Biomedical Sciences, 1994, East Tennessee State University

URL: https://dc.etsu.edu/etd/2892

► Although tachykinins have potent vascular actions, their effect on renal resistance blood vessels is currently unknown. The vasoactive properties of tachykinins and related analogs… (more)

▼ Although tachykinins have potent vascular actions, their effect on renal resistance blood vessels is currently unknown. The vasoactive properties of tachykinins and related analogs were assessed in isolated perfused rat kidney. At a basal perfusion pressure (PP) of 75 ± 6 mm Hg (n = 5), bolus injections of substance P (SP) had no significant vasoactive effect. Following a sustained increase in baseline PP (134 ± 10 mm Hg) produced by phenylephrine (1 μM), SP evoked a dose-dependent increase in PP. The largest dose of SP increased PP by 60 ± 5 mm Hg. The vasoconstrictor response to SP was not blocked by phentolamine when angiotensin II was used to increase basal tone. Thus, the response to SP is not mediated by norepinephrine. Pressor responses to SP were not potentiated by peptidase inhibitors, captopril and thiorphan. SP(1-7) had no effect on PP, suggesting that the pressor response to SP is C-terminal dependent and tachykinin receptor mediated. The selective NK-1 receptor agonist, (Sar⁹,Met(O₂)¹¹]SP, had no effect on PP. In contrast, both the selective NK-2 and NK-3 receptor agonists, GR-64349 and (MePhe⁷) NKB, produced dose-dependent pressor responses (116 ± 8 and 134 ± 15 mm Hg increases in PP at 33 nmol, respectively) and were more potent than SP. Infusion of capsaicin (500 nM) produced an initial increase in PP following by a more prolonged decrease in PP. Clamping the renal vein produced a marked increase in PP. The localization of NK-3 receptors in rat kidney evaluated by film autoradiography using ¹²⁵I- (MePhe⁷]NKB revealed a high density of specific binding sites on the proximal ureter and renal pelvis, moderate density in the renal vein and its large branches, and a low density in the inner strip of outer medulla, but no specific binding on the renal artery system and cortex. High resolution autoradiograms demonstrated ¹²⁵I- (MePhe⁷]NKB binding sites on the tunica media of the renal vein and tunica muscularises of renal pelvis and ureter. Specific binding of ¹²⁵I-BHSP was found in association with the renal artery and renal pelvis. No specific SP binding sites were associated with renal vein. These data indicate that the pressor effect of tachykinins in the isolated rat kidney can be mediated by NK-2 and/or NK-3 receptors. The latter may be on the vascular smooth muscle of the renal vein.

Subjects/Keywords: Anatomy and physiology; Animals; Biological sciences; Biophysics; Health and environmental sciences; Pharmacology; Vasoactive; Anatomy; Animal Structures; Biophysics; Pharmacology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chen, Y. (1994). Characterization of the Vasoactivity of Tachykinins in Isolated Rat Kidney: Functional Studies and in Vitro Receptor Autoradiography. (Doctoral Dissertation). East Tennessee State University. Retrieved from https://dc.etsu.edu/etd/2892

Chicago Manual of Style (16^th Edition):

Chen, Yuejin. “Characterization of the Vasoactivity of Tachykinins in Isolated Rat Kidney: Functional Studies and in Vitro Receptor Autoradiography.” 1994. Doctoral Dissertation, East Tennessee State University. Accessed December 06, 2019. https://dc.etsu.edu/etd/2892.

MLA Handbook (7^th Edition):

Chen, Yuejin. “Characterization of the Vasoactivity of Tachykinins in Isolated Rat Kidney: Functional Studies and in Vitro Receptor Autoradiography.” 1994. Web. 06 Dec 2019.

Vancouver:

Chen Y. Characterization of the Vasoactivity of Tachykinins in Isolated Rat Kidney: Functional Studies and in Vitro Receptor Autoradiography. [Internet] [Doctoral dissertation]. East Tennessee State University; 1994. [cited 2019 Dec 06]. Available from: https://dc.etsu.edu/etd/2892.

Council of Science Editors:

Chen Y. Characterization of the Vasoactivity of Tachykinins in Isolated Rat Kidney: Functional Studies and in Vitro Receptor Autoradiography. [Doctoral Dissertation]. East Tennessee State University; 1994. Available from: https://dc.etsu.edu/etd/2892

East Tennessee State University

25. Cheng, Hong. Quantal Mechanisms Underlying Stimulation-induced Augmentation and Potentiation.

Degree: PhD, Biomedical Sciences, 1998, East Tennessee State University

URL: https://dc.etsu.edu/etd/2893

► Repetitive stimulation of motor nerves causes an increase in the number of packets of transmitter ("quanta") that can be released in the ensuing period.… (more)

▼ Repetitive stimulation of motor nerves causes an increase in the number of packets of transmitter ("quanta") that can be released in the ensuing period. This represents a type of conditioning, in which synaptic transmission may be enhanced by prior activity. Despite many studies of this phenomenon, there have been no investigations of the quantal mechanisms underlying these events, due to the rapid changes in transmitter output and the short time periods involved. To examine this problem, a method was developed in which estimates of the quantal release parameters could be obtained over very brief periods (3 s). Conventional microelectrode techniques were used to record miniature endplate potentials (MEPPs) from isolated frog (Rana pipiens) cutaneous pectoris muscles, before and after repetitive (40 sec at 80 Hz) nerve stimulation. Estimates were obtained of m (number of quanta released), n (number of functional release sites), p (mean probability of release) and var_sp (spatial variance in p) using a method that employs counts of MEPPs per unit time. Fluctuations in the estimates were reduced using a moving bin technique (bin size = 3 s, Δbin = 1 s). Muscle contraction was prevented using low Ca²⁺, high Mg²⁺ Ringer or normal Ringer to which μ-conotoxin GIIIA was added. These studies showed that: (1) the post-stimulation increase in transmitter release was dependent on stimulation frequency and not on the total number of stimulus impulses. When the total number of pulses was kept constant, the high frequency pattern produced a higher level of transmitter release than did the lower frequency patterns; (2) augmentation and potentiation were present in both low Ca²⁺, high Mg²⁺ and normal Ringer solutions, but potentiation, m, n, p and var_sp were greater in normal Ringer solution than in low Ca²⁺, high Mg²⁺ solution. In low Ca²⁺, high Mg²⁺ solution, there was a larger decrease in n compared to p; (3) hypertonicity (addition of 100 mM sucrose) produced a marked increase in both basal and stimulation-induced values of m, n, and p. By contrast, there was a marked increase in the stimulation-induced but not the basal values of var_sp; (4) hypertonicity produced a decrease in augmentation but had no effect on potentiation; (5) augmentation and potentiation appeared to involve mitochondrial uptake and efflux of cytoplasmic Ca²⁺. Tetraphenylphosphonium (which blocks mitochondrial Ca²⁺ efflux and uptake) decreased augmentation and potentiation in low Ca²⁺, high Mg²⁺ solutions but increased potentiation in the same solution made hypertonic with 100 mM sucrose; (6) the overall findings suggest that this new method may be useful for investigating the subcellular dynamics of transmitter release following nerve stimulation.

Subjects/Keywords: Anatomy and physiology; Animals; Augmentation; Biological sciences; Hypertonicity; Miniature endplate potentials; Neurology; Potentiation; Quantal mechanisms; Stimulation-induced; Anatomy; Animal Structures; Neurology

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APA (6^th Edition):

Cheng, H. (1998). Quantal Mechanisms Underlying Stimulation-induced Augmentation and Potentiation. (Doctoral Dissertation). East Tennessee State University. Retrieved from https://dc.etsu.edu/etd/2893

Chicago Manual of Style (16^th Edition):

Cheng, Hong. “Quantal Mechanisms Underlying Stimulation-induced Augmentation and Potentiation.” 1998. Doctoral Dissertation, East Tennessee State University. Accessed December 06, 2019. https://dc.etsu.edu/etd/2893.

MLA Handbook (7^th Edition):

Cheng, Hong. “Quantal Mechanisms Underlying Stimulation-induced Augmentation and Potentiation.” 1998. Web. 06 Dec 2019.

Vancouver:

Cheng H. Quantal Mechanisms Underlying Stimulation-induced Augmentation and Potentiation. [Internet] [Doctoral dissertation]. East Tennessee State University; 1998. [cited 2019 Dec 06]. Available from: https://dc.etsu.edu/etd/2893.

Council of Science Editors:

Cheng H. Quantal Mechanisms Underlying Stimulation-induced Augmentation and Potentiation. [Doctoral Dissertation]. East Tennessee State University; 1998. Available from: https://dc.etsu.edu/etd/2893

East Tennessee State University

26. King, Judy A. Adaptation of Striped Bass to Sea Water Following Direct Transfer from Freshwater: Morphological, Biochemical, and Physiological Parameters.

Degree: PhD, Biomedical Sciences, 1987, East Tennessee State University

URL: https://dc.etsu.edu/etd/2932

► There has been heightened interest in the biology of striped bass (Morone saxatilis) because of increased pollution in their native spawning grounds and because… (more)

▼ There has been heightened interest in the biology of striped bass (Morone saxatilis) because of increased pollution in their native spawning grounds and because of their extensive use in landlocked sport fisheries. Their euryhalinity makes them an excellent species for osmoregulation studies. The objective of this research was to study the rate of adaptation of striped bass gills to sea water (3% salt) after direct transfer from freshwater using biochemical (ion transport enzyme levels), physiological (chloride efflux), and ultrastructural methods. Striped bass have specialized osmoregulatory cells located on the interlamellar and afferent surfaces of their gill filaments as shown by light microscopy (LM), transmission electron microscopy (TEM), and scanning electron microscopy (SEM). SEM studies show that apical pit (opening of one or more chloride cells) morphology changes during sea water adaptation, and the number of apical pits increases by 32.5% after two weeks in sea water. Chloride cell size and number, extent of basolateral tubular system, and number of mitochondria per chloride cell appear to increase upon adaptation to sea water. Sodium-potassium adenosine triphosphatase (Na,K-ATPase) activity is maximal on day 3 after transfer to sea water. Studies suggest that cortisol may act as a hormonal mediator for long term adaptation to sea water. The general morphology of both freshwater and sea water adapted fish gills were studied. Preliminary studies indicate that the osmium-dimethylsulfoxide-osmium method can be used to investigate intracellular structural changes in striped bass gills. Since the chloride cells are associated with the afferent surface of the filament, the blood supply to that area is also of great interest in osmoregulation studies. Studies of the gill vasculature using corrosion casting (i.e. filling blood vessels with plastic resins) and SEM indicate that the blood vessel distribution in the striped bass gill is similar to that of other euryhaline species with arterio-arterial, arterio-venous, and nutritive pathways. Blood flow may be controlled at a variety of places by sphincters, shunts and cellular contraction. Correlation of these biochemical, physiological and anatomical measurements will aid in the understanding of the process of adaptation to sea water. (Abstract shortened with permission of author.)

Subjects/Keywords: Anatomy and physiology; Aquaculture; Biological sciences; Biology; Fish production; Zoology; Anatomy; Aquaculture and Fisheries; Biology; Physiology; Zoology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

King, J. A. (1987). Adaptation of Striped Bass to Sea Water Following Direct Transfer from Freshwater: Morphological, Biochemical, and Physiological Parameters. (Doctoral Dissertation). East Tennessee State University. Retrieved from https://dc.etsu.edu/etd/2932

Chicago Manual of Style (16^th Edition):

King, Judy A. “Adaptation of Striped Bass to Sea Water Following Direct Transfer from Freshwater: Morphological, Biochemical, and Physiological Parameters.” 1987. Doctoral Dissertation, East Tennessee State University. Accessed December 06, 2019. https://dc.etsu.edu/etd/2932.

MLA Handbook (7^th Edition):

King, Judy A. “Adaptation of Striped Bass to Sea Water Following Direct Transfer from Freshwater: Morphological, Biochemical, and Physiological Parameters.” 1987. Web. 06 Dec 2019.

Vancouver:

King JA. Adaptation of Striped Bass to Sea Water Following Direct Transfer from Freshwater: Morphological, Biochemical, and Physiological Parameters. [Internet] [Doctoral dissertation]. East Tennessee State University; 1987. [cited 2019 Dec 06]. Available from: https://dc.etsu.edu/etd/2932.

Council of Science Editors:

King JA. Adaptation of Striped Bass to Sea Water Following Direct Transfer from Freshwater: Morphological, Biochemical, and Physiological Parameters. [Doctoral Dissertation]. East Tennessee State University; 1987. Available from: https://dc.etsu.edu/etd/2932

University of California – San Francisco

27. Behonick, Danielle Janine. A Study of MMPs During Skeletal Development and Repair.

Degree: Biomedical Sciences, 2007, University of California – San Francisco

URL: http://www.escholarship.org/uc/item/2qs377z5

► The extracellular matrix (ECM) is an essential player in functions including cell survival, migration and differentiation. It is a dynamic reflection of the state of… (more)

Subjects/Keywords: Biology, Anatomy; Biology, Cell; MMP; collagen; chondrocyte; osteoblast; skeletal development; fracture repair

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Behonick, D. J. (2007). A Study of MMPs During Skeletal Development and Repair. (Thesis). University of California – San Francisco. Retrieved from http://www.escholarship.org/uc/item/2qs377z5

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Behonick, Danielle Janine. “A Study of MMPs During Skeletal Development and Repair.” 2007. Thesis, University of California – San Francisco. Accessed December 06, 2019. http://www.escholarship.org/uc/item/2qs377z5.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Behonick, Danielle Janine. “A Study of MMPs During Skeletal Development and Repair.” 2007. Web. 06 Dec 2019.

Vancouver:

Behonick DJ. A Study of MMPs During Skeletal Development and Repair. [Internet] [Thesis]. University of California – San Francisco; 2007. [cited 2019 Dec 06]. Available from: http://www.escholarship.org/uc/item/2qs377z5.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Behonick DJ. A Study of MMPs During Skeletal Development and Repair. [Thesis]. University of California – San Francisco; 2007. Available from: http://www.escholarship.org/uc/item/2qs377z5

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

East Tennessee State University

28. Long, Tessa L. In Vitro Assessment of the Toxicity of Cocaine and Its Metabolites in the Human Umbilical Artery.

Degree: PhD, Biomedical Sciences, 1998, East Tennessee State University

URL: https://dc.etsu.edu/etd/2941

► An in vitro model was used to assess the effect of cocaine and its metabolites on the umbilical artery. Objectives were to pharmacologically confirm… (more)

▼ An in vitro model was used to assess the effect of cocaine and its metabolites on the umbilical artery. Objectives were to pharmacologically confirm the presence of adrenergic innervation using tyramine, evaluate the ability of cocaine, benzoylecgonine, norcocaine and cocaethylene to potentiate vasoconstriction by serotonin and norepinephrine, examine the ability of ketanserin to block the enhanced vasoconstriction produced by cocaine, and determine displacement of 3 H-ketanserin by serotonin, norepinephrine, tyramine and mianserin. The vasoconstrictive effect of tyramine (100 μM) was enhanced in the presence of cocaine by 257%. Vasoconstrictive effects of serotonin and norepinephrine were significantly enhanced by cocaine by 28%, and 64% respectively; producing significant increases in the cumulative response. Norcocaine significantly augmented the maximum response to norepinephrine by 54%. Benzoylecgonine significantly decreased the maximum response to serotonin by 36% as well as the cumulative response. Ketanserin (0.03 μM) completely attenuated the vasoconstrictive potentiation of serotonin and norepinephrine by cocaine; shifting the EC50 for serotonin to the right 10-fold in the presence of ketanserin and cocaine. Ketanserin shifted the EC15 for norepinephrine with cocaine to the right 205-fold. Maximum response to norepinephrine with cocaine was depressed 54% by ketanserin. Serotonin, tyramine, and mianserin were able to displace 3 H-ketanserin (3 nM) from the membrane fraction of the human umbilical artery. Indicating that serotonin2 receptors are involved in vasoconstrictive responses to serotonin and tyramine. Norepinephrine did not displace 3 H-ketanserin in the membrane fraction of the umbilical artery. These data suggest that enhanced vasoconstriction of norepinephrine and serotonin by cocaine and potentiation of the maximum response to norepinephrine by norcocaine in the human umbilical artery may be important components of perinatal cocaine toxicity. Ketanserin was able to suppress the umbilical artery constriction produced by cocaine, demonstrating its antidotal potential. The potentiation of the tyramine response by cocaine and the displacement of 3 H-ketanserin by tyramine indicate that tyramine may be producing its vasoconstrictive effect through serotonin2 in the umbilical artery.

Subjects/Keywords: Anatomy and physiology; Animals; Biological sciences; Cocaine; Health and environmental sciences; Metabolites; Molecular biology; Toxicity; Toxicology; Umbilical artery; Anatomy; Animal Structures; Molecular Biology; Toxicology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Long, T. L. (1998). In Vitro Assessment of the Toxicity of Cocaine and Its Metabolites in the Human Umbilical Artery. (Doctoral Dissertation). East Tennessee State University. Retrieved from https://dc.etsu.edu/etd/2941

Chicago Manual of Style (16^th Edition):

Long, Tessa L. “In Vitro Assessment of the Toxicity of Cocaine and Its Metabolites in the Human Umbilical Artery.” 1998. Doctoral Dissertation, East Tennessee State University. Accessed December 06, 2019. https://dc.etsu.edu/etd/2941.

MLA Handbook (7^th Edition):

Long, Tessa L. “In Vitro Assessment of the Toxicity of Cocaine and Its Metabolites in the Human Umbilical Artery.” 1998. Web. 06 Dec 2019.

Vancouver:

Long TL. In Vitro Assessment of the Toxicity of Cocaine and Its Metabolites in the Human Umbilical Artery. [Internet] [Doctoral dissertation]. East Tennessee State University; 1998. [cited 2019 Dec 06]. Available from: https://dc.etsu.edu/etd/2941.

Council of Science Editors:

Long TL. In Vitro Assessment of the Toxicity of Cocaine and Its Metabolites in the Human Umbilical Artery. [Doctoral Dissertation]. East Tennessee State University; 1998. Available from: https://dc.etsu.edu/etd/2941

East Tennessee State University

29. Mabe, Abigail Marie. Characterization of Murine Cardiac Cholinergic Innervation and Its Remodeling in Type 1 Diabetes.

Degree: PhD, Biomedical Sciences, 2008, East Tennessee State University

URL: https://dc.etsu.edu/etd/2009

► Murine models have become increasingly popular to study various aspects of cardiovascular diseases due to their ease of genetic manipulation. Unfortunately, there has been… (more)

▼ Murine models have become increasingly popular to study various aspects of cardiovascular diseases due to their ease of genetic manipulation. Unfortunately, there has been little effort put into describing the distribution of autonomic nerves in the mouse heart, making it difficult to compare current findings from clinical and experimental models related to cardiovascular diseases. Furthermore, determination of the requirements for the development of this system and its maintenance in adult mice remains largely unexplored. This study represents the first detailed mapping of cholinergic neuroanatomy of the mouse heart based on immunohistochemical staining using true cholinergic markers. We found cholinergic innervation of the mouse heart to be largely focused in the atrium and conducting system. We investigated the involvement of the neurotrophic factor neurturin (NRTN) in the development of cholinergic innervation, because there was indirect evidence that implicated it as a crucial factor. Results from our work definitively demonstrate that NRTN plays a major role in the development of cardiac parasympathetic ganglia and cholinergic innervation of the mouse heart. Adult NRTN knockout mice exhibited a drastic reduction in the number of intracardiac neurons with decreased atrial acetylcholine, cholinergic nerve density at the sinoatrial node and negative chronotropic responses to vagal stimulation. The presence of NRTN and its receptors in hearts from adult wild-type mice suggests that this neurotrophic factor might also be required for maintenance of cardiac cholinergic innervation. Finally, we wanted to determine how intracardiac neurons and their processes change during diseased states, specifically type 1 diabetes. This work has shown that the cardiac cholinergic nervous system in the mouse undergoes structural and functional remodeling when challenged with streptozotocin-induced diabetes. Cholinergic nerves in diabetic hearts undergo extensive sprouting at the sinoatrial node with no change in the number of intracardiac neurons. Cholinergic function appears to be enhanced in diabetic mice, based on pharmacological testing, despite decreased response to direct vagal nerve stimulation. Evidence also suggests that diabetic mice have an imbalance in autonomic control of heart rate. The latter findings suggest that disruption of central input into intrinsic cardiac ganglia also contributes to the neuropathology of type 1 diabetes.

Subjects/Keywords: neurotrophic factor; parasympathetic; intrinsic cardiac neuron; autonomic nervous system; streptozotocin; diabetes; Anatomy; Cardiovascular System; Medicine and Health Sciences; Nervous System

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mabe, A. M. (2008). Characterization of Murine Cardiac Cholinergic Innervation and Its Remodeling in Type 1 Diabetes. (Doctoral Dissertation). East Tennessee State University. Retrieved from https://dc.etsu.edu/etd/2009

Chicago Manual of Style (16^th Edition):

Mabe, Abigail Marie. “Characterization of Murine Cardiac Cholinergic Innervation and Its Remodeling in Type 1 Diabetes.” 2008. Doctoral Dissertation, East Tennessee State University. Accessed December 06, 2019. https://dc.etsu.edu/etd/2009.

MLA Handbook (7^th Edition):

Mabe, Abigail Marie. “Characterization of Murine Cardiac Cholinergic Innervation and Its Remodeling in Type 1 Diabetes.” 2008. Web. 06 Dec 2019.

Vancouver:

Mabe AM. Characterization of Murine Cardiac Cholinergic Innervation and Its Remodeling in Type 1 Diabetes. [Internet] [Doctoral dissertation]. East Tennessee State University; 2008. [cited 2019 Dec 06]. Available from: https://dc.etsu.edu/etd/2009.

Council of Science Editors:

Mabe AM. Characterization of Murine Cardiac Cholinergic Innervation and Its Remodeling in Type 1 Diabetes. [Doctoral Dissertation]. East Tennessee State University; 2008. Available from: https://dc.etsu.edu/etd/2009

Stellenbosch University

30. Van Wyk, Christine. Ancillary methods to improve diagnostic accuracy of thyroid nodules on fine-needle aspiration cytology smears.

Degree: MScMed (Dept. of Biomedical Sciences. Anatomy and Histology, Biomedical Sciences, 2007, Stellenbosch University

URL: http://hdl.handle.net/10019.1/2749

► Thyroid nodules are a common clinical problem encountered by physicians, surgeons and radiologists who deal with the head and neck region. However, most follicular lesions… (more)

▼ Thyroid nodules are a common clinical problem encountered by physicians, surgeons and radiologists who deal with the head and neck region. However, most follicular lesions of the thyroid are benign, so that the indications for surgery should be as accurate as possible. The morphological difficulty on fine-needle aspiration biopsy (FNAB) of reliably distinguishing preoperatively between benign and malignant lesions has led to a search for ancillary methods that can assist with the diagnosis. The aim of the first study was to develop a cytological scoring system to improve diagnostic accuracy of fine-needle aspiration biopsy of papillary carcinomas with special reference to the follicular variant of papillary carcinoma. The objective of the second study was the application of immunodiagnostic markers Galectin-3 and HBME-1 to histology tissue sections and their corresponding fine-needle aspiration cytology smears to assess their value in distinguishing benign from malignant thyroid lesions. In the first study 16 different cytological features such as background, architecture and cellular morphology were quantatively assessed and scored. Only 14 of the 16 variables were statistically significant. The statistical analysis demonstrated that a score ≥ 4 was indicative of a papillary carcinoma with a sensitivity of 96%. A score < 4 suggested a benign multinodular goiter with a specificity of 97%. In the second study Galectin-3 and HBME-1 were applied to histology tissue sections and their corresponding fine-needle aspiration cytology smears. Statistical analyses showed that the sensitivity of immunohistochemistry for diagnosing malignancy was better than the immunocytochemistry, but the specificity of immunocytochemistry was superior. Furthermore the diagnostic accuracy of immunohistochemistry (86%) and immunocytochemistry (88%) using co-expression of these two antibodies was excellent. In this study on immunocytochemistry, papillary carcinomas were clearly identified with a 100% co-expression in the classic and 71% in the follicular variant of papillary carcinoma. For the surgeon the identification of papillary carcinoma is critical, as this determines the extent of surgery. Similary, the confirmation of a non-neoplastic lesion may prevent surgery. In most cases follicular neoplasms, benign or malignant, will usually be excised for histopathology, prior to definite therapy. These studies show that the implementation of ancillary methods such as a scoring system and immunodiagnostic markers can improve the diagnostic accuracy of thyroid fine-needle aspiration biopsies in our laboratory. This may lead to better management of thyroid nodules. However, it is advisable that cytopathologists always take all the clinical features and image analyses into consideration before making a diagnosis. Advisors/Committee Members: Louw, Mercia, Wright, Colleen, University of Stellenbosch. Faculty of Health Sciences. Dept. of Biomedical Sciences. Anatomy and Histology..

Subjects/Keywords: Anatomy and histology; Thyroid gland – Cytology – Technique; Thyroid gland – Histology; Thyroid gland – Needle biopsy – Technique

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Van Wyk, C. (2007). Ancillary methods to improve diagnostic accuracy of thyroid nodules on fine-needle aspiration cytology smears. (Masters Thesis). Stellenbosch University. Retrieved from http://hdl.handle.net/10019.1/2749

Chicago Manual of Style (16^th Edition):

Van Wyk, Christine. “Ancillary methods to improve diagnostic accuracy of thyroid nodules on fine-needle aspiration cytology smears.” 2007. Masters Thesis, Stellenbosch University. Accessed December 06, 2019. http://hdl.handle.net/10019.1/2749.

MLA Handbook (7^th Edition):

Van Wyk, Christine. “Ancillary methods to improve diagnostic accuracy of thyroid nodules on fine-needle aspiration cytology smears.” 2007. Web. 06 Dec 2019.

Vancouver:

Van Wyk C. Ancillary methods to improve diagnostic accuracy of thyroid nodules on fine-needle aspiration cytology smears. [Internet] [Masters thesis]. Stellenbosch University; 2007. [cited 2019 Dec 06]. Available from: http://hdl.handle.net/10019.1/2749.

Council of Science Editors:

Van Wyk C. Ancillary methods to improve diagnostic accuracy of thyroid nodules on fine-needle aspiration cytology smears. [Masters Thesis]. Stellenbosch University; 2007. Available from: http://hdl.handle.net/10019.1/2749

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