subject:( pathogenicity )

University of Utah

1. Arrington, Blythe. Cloning and characterization of an iron-uptake ATP-binding cassette (ABC) transporter of Bartonella henselae;.

Degree: MS;, Pathology;, 2001, University of Utah

URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1249/rec/169

► Bartonella henselae is a fastidious gram-negative, slow-growing organism that is a main causative agent of cat scratch disease. Cat scratch disease is characterized by the… (more)

Subjects/Keywords: Pathogenicity; Metabolism

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APA (6^th Edition):

Arrington, B. (2001). Cloning and characterization of an iron-uptake ATP-binding cassette (ABC) transporter of Bartonella henselae;. (Masters Thesis). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1249/rec/169

Chicago Manual of Style (16^th Edition):

Arrington, Blythe. “Cloning and characterization of an iron-uptake ATP-binding cassette (ABC) transporter of Bartonella henselae;.” 2001. Masters Thesis, University of Utah. Accessed March 03, 2021. http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1249/rec/169.

MLA Handbook (7^th Edition):

Arrington, Blythe. “Cloning and characterization of an iron-uptake ATP-binding cassette (ABC) transporter of Bartonella henselae;.” 2001. Web. 03 Mar 2021.

Vancouver:

Arrington B. Cloning and characterization of an iron-uptake ATP-binding cassette (ABC) transporter of Bartonella henselae;. [Internet] [Masters thesis]. University of Utah; 2001. [cited 2021 Mar 03]. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1249/rec/169.

Council of Science Editors:

Arrington B. Cloning and characterization of an iron-uptake ATP-binding cassette (ABC) transporter of Bartonella henselae;. [Masters Thesis]. University of Utah; 2001. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1249/rec/169

University of Utah

2. Slhokoohi, Vida. Identification and characterizations of gonococci type 5: one step forward in understanding the attachment of gonococci to mucosal cells.

Degree: MS;, Pathology;, 1986, University of Utah

URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1420/rec/601

► The characteristic of the organisms from gonococcal type 5 morphology and their interaction with human epithelial cells are least studied among different types of gonococci.… (more)

Subjects/Keywords: Pathology; Pathogenicity

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APA (6^th Edition):

Slhokoohi, V. (1986). Identification and characterizations of gonococci type 5: one step forward in understanding the attachment of gonococci to mucosal cells. (Masters Thesis). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1420/rec/601

Chicago Manual of Style (16^th Edition):

Slhokoohi, Vida. “Identification and characterizations of gonococci type 5: one step forward in understanding the attachment of gonococci to mucosal cells.” 1986. Masters Thesis, University of Utah. Accessed March 03, 2021. http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1420/rec/601.

MLA Handbook (7^th Edition):

Slhokoohi, Vida. “Identification and characterizations of gonococci type 5: one step forward in understanding the attachment of gonococci to mucosal cells.” 1986. Web. 03 Mar 2021.

Vancouver:

Slhokoohi V. Identification and characterizations of gonococci type 5: one step forward in understanding the attachment of gonococci to mucosal cells. [Internet] [Masters thesis]. University of Utah; 1986. [cited 2021 Mar 03]. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1420/rec/601.

Council of Science Editors:

Slhokoohi V. Identification and characterizations of gonococci type 5: one step forward in understanding the attachment of gonococci to mucosal cells. [Masters Thesis]. University of Utah; 1986. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1420/rec/601

3. Smati, Mounira. Place de la structure génétique de l'espèce Escherichia coli dans l'état de son commensalisme intestinal et dans l'expression de sa virulence. : Impact of the genetic structure of Escherichia coli species in intestinal commensalism and in virulence expression.

Degree: Docteur es, Biologie moléculaire, 2014, Paris 13

URL: http://www.theses.fr/2014PA132041

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Escherichia coli est le commensal aérobie le plus fréquent du tube digestif de l’homme et des animaux à sang chaud et le bacille à Gram… (more)

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Escherichia coli est le commensal aérobie le plus fréquent du tube digestif de l’homme et des animaux à sang chaud et le bacille à Gram négatif pathogène opportuniste le plus souvent impliqué dans les infections intestinales et extra intestinales de l’homme. C’est une espèce clonale chez laquelle 4 groupes phylogénétiques principaux, A, B1, B2 et D ont été décrits. L’objectif de cette thèse est d’étudier l’adaptation de E. coli et les rapports de cette adaptation avec la structure génétique de l’espèce caractérisée par les 4 groupes phylogénétiques dans deux circonstances : le commensalisme intestinal de l’homme et de plusieurs espèces animales sauvages et domestiques, herbivores et omnivores d’une part et la virulence extra-intestinale mesurée par l’expression des gènes codants pour un sidérophore, la yersiniabactine, dont les gènes sont situés au sein de l’ilot de pathogénicité HPI (PAI IV). La répartition dans les 4 groupes phylogénétiques des souches commensales du tube digestif de 100 hommes et de 137 animaux a été étudiée par une technique de PCR en temps réel originale. Trois principaux entérocolitypes, correspondant à des associations préférentielles de phylogroupes ont été ainsi décrits comme plus fréquents en fonction de la nature des hôtes.Chez l’homme, les souches du groupe B2 ont été retrouvés exclusives chez 15 % des individus et ont été clairement distinctes des souches B2 des animaux sauvages par la plus grande fréquence de leurs facteurs de virulences (sfa/foc et pks). L’effet du fond génétique des sous groupes II, III et IX du groupe B2 sur l’expression de la virulence liée au HPI a été étudié dans un modèle murin de virulence extra-intestinale et dans un modèle d’amibe sociale Dictyostelium discoideum, pouvant être assimilé à un macrophage. Le HPI chez E. coli interagît avec la clonalité de l’espèce qui s’exprime par l’existence des sous-groupes de B2. Dans les modèles de virulence que nous avons développés, les mêmes gènes ont, en fonction du fond génétique des différents isolats naturels, des effets différents.

Escherichia coli is the most abundant aerobic bacteria of the human microbiota, and a major opportunistic pathogen in humans. It is the clonal species for wich main phylogenetic groups have been described. The aim of this thesis is to study E. coli adaptation through the genetic structure of the specis in two circumstances : the intestinal comensalism, and the extra-intestinal virulence estimated via expression of genes encoding for yersiniabactin, a major siderophore, located on a high patogenicity island (HPI). The repartition of the 4 phylogroups has been studied in faecal microbiota of 100 humans and 137 animals thanks to an original quantitative PCR assay. Three main enterocolitypes, corresponding to associations of phylogroups, have been described. In humans, B2 phylogroup strains were exclusive in 15% of individuals and were shown to be clearly distinct from animal B2 strains on the base of the presence of two virulence factors (sfa/foc and pks). The impact of the genetic…

Advisors/Committee Members: Picard, Bertrand (thesis director), Denamur, Erick (thesis director).

Subjects/Keywords: Ilot de pathogenicité; Pathogenicity island

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APA (6^th Edition):

Smati, M. (2014). Place de la structure génétique de l'espèce Escherichia coli dans l'état de son commensalisme intestinal et dans l'expression de sa virulence. : Impact of the genetic structure of Escherichia coli species in intestinal commensalism and in virulence expression. (Doctoral Dissertation). Paris 13. Retrieved from http://www.theses.fr/2014PA132041

Chicago Manual of Style (16^th Edition):

Smati, Mounira. “Place de la structure génétique de l'espèce Escherichia coli dans l'état de son commensalisme intestinal et dans l'expression de sa virulence. : Impact of the genetic structure of Escherichia coli species in intestinal commensalism and in virulence expression.” 2014. Doctoral Dissertation, Paris 13. Accessed March 03, 2021. http://www.theses.fr/2014PA132041.

MLA Handbook (7^th Edition):

Smati, Mounira. “Place de la structure génétique de l'espèce Escherichia coli dans l'état de son commensalisme intestinal et dans l'expression de sa virulence. : Impact of the genetic structure of Escherichia coli species in intestinal commensalism and in virulence expression.” 2014. Web. 03 Mar 2021.

Vancouver:

Smati M. Place de la structure génétique de l'espèce Escherichia coli dans l'état de son commensalisme intestinal et dans l'expression de sa virulence. : Impact of the genetic structure of Escherichia coli species in intestinal commensalism and in virulence expression. [Internet] [Doctoral dissertation]. Paris 13; 2014. [cited 2021 Mar 03]. Available from: http://www.theses.fr/2014PA132041.

Council of Science Editors:

Smati M. Place de la structure génétique de l'espèce Escherichia coli dans l'état de son commensalisme intestinal et dans l'expression de sa virulence. : Impact of the genetic structure of Escherichia coli species in intestinal commensalism and in virulence expression. [Doctoral Dissertation]. Paris 13; 2014. Available from: http://www.theses.fr/2014PA132041

University of Utah

4. Seiler, Kathleen Marie Petri. Investigation of the pathogenesis of murine Lyme arthritis;.

Degree: PhD, Pathology;, 1997, University of Utah

URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/969/rec/722

► Lyme disease is caused by the spirochete Borrelia burgdorferi and if left untreated, often causes arthritis. The spirochetes possess outer surface lipoproteins (Osps) that are… (more)

▼ Lyme disease is caused by the spirochete Borrelia burgdorferi and if left untreated, often causes arthritis. The spirochetes possess outer surface lipoproteins (Osps) that are capable of stimulating inflammatory cytokine production from B cells, macrophages, and endothelial cells, and the production of nitric oxide (NO). The role of NO during infection of mice with B. burgdorferi was investigated. Both the severely arthritic (C3H) and mildly arthritic (BALB/c) mice produced high levels of NO approximately 7 days after infection, but C3H animals rapidly downregulated NO production to background levels, whereas BALB/c animals sustained NO production throughout the course of infection. Complete inhibition of NO production by the compound N[G]-monomethyl-L-arginine (LMMA) during infection had no significant effect on either arthritis development or spirochete numbers in tissues. These results indicate that NO is ineffective at controlling spirochete numbers in tissues, and, therefore, B. burgdorferi probably does not require an intracellular localization for survival in the mammalian host. The reproducible downregulation of NO in C3H animals may be induced by anti-inflammatory cytokines which could modulate other aspects of the immune response to B. burgdorferi. In vitro studies showed that OspA could stimulate IL-10 production by naive murine splenocytes and that physiological concentrations of IL-10 could significantly downregulate the responses of OspA-treated macrophages. The inappropriate or uncontrolled production of IL-10 during infection could result in dysregulation of host defenses, thus leading to the development of pathology. The Osps also stimulate adhesion molecule expression on cells. The role of two adhesion molecules, E-selectin and P-selectin, in the development of Lyme arthritis, was investigated by infecting mice lacking both molecules with B. burgdorferi and assessing arthritis development and spirochete number. Mice lacking E- and P-selectin showed resistance to arthritis development and spirochete numbers virtually identical to their wild-type littermates, indicating that these adhesion molecules are not required for resistance to Lyme arthritis. Additionally, a correlation between influx of neutrophils into joints and arthritis severity was noted, indicating that neutrophil infiltration into joints may be required for the development of arthritis. These studies may aid in the determination of pathogenic mechanisms in Lyme arthritis.

Subjects/Keywords: Pathogenicity; Complications

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APA (6^th Edition):

Seiler, K. M. P. (1997). Investigation of the pathogenesis of murine Lyme arthritis;. (Doctoral Dissertation). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/969/rec/722

Chicago Manual of Style (16^th Edition):

Seiler, Kathleen Marie Petri. “Investigation of the pathogenesis of murine Lyme arthritis;.” 1997. Doctoral Dissertation, University of Utah. Accessed March 03, 2021. http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/969/rec/722.

MLA Handbook (7^th Edition):

Seiler, Kathleen Marie Petri. “Investigation of the pathogenesis of murine Lyme arthritis;.” 1997. Web. 03 Mar 2021.

Vancouver:

Seiler KMP. Investigation of the pathogenesis of murine Lyme arthritis;. [Internet] [Doctoral dissertation]. University of Utah; 1997. [cited 2021 Mar 03]. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/969/rec/722.

Council of Science Editors:

Seiler KMP. Investigation of the pathogenesis of murine Lyme arthritis;. [Doctoral Dissertation]. University of Utah; 1997. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/969/rec/722

Vanderbilt University

5. Shaffer, Carrie Leigh. Role of unique Helicobacter pylori proteins in the Cag pathogenicity island-encoded type IV secretion system.

Degree: PhD, Microbiology and Immunology, 2011, Vanderbilt University

URL: http://hdl.handle.net/1803/14725

► Colonization of the human stomach by Helicobacter pylori is an important risk factor for development of gastric cancer. The H. pylori cag pathogenicity island (cag… (more)

Subjects/Keywords: bacteriology; proteomics; Helicobacter pylori; pathogenicity

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Shaffer, C. L. (2011). Role of unique Helicobacter pylori proteins in the Cag pathogenicity island-encoded type IV secretion system. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/14725

Chicago Manual of Style (16^th Edition):

Shaffer, Carrie Leigh. “Role of unique Helicobacter pylori proteins in the Cag pathogenicity island-encoded type IV secretion system.” 2011. Doctoral Dissertation, Vanderbilt University. Accessed March 03, 2021. http://hdl.handle.net/1803/14725.

MLA Handbook (7^th Edition):

Shaffer, Carrie Leigh. “Role of unique Helicobacter pylori proteins in the Cag pathogenicity island-encoded type IV secretion system.” 2011. Web. 03 Mar 2021.

Vancouver:

Shaffer CL. Role of unique Helicobacter pylori proteins in the Cag pathogenicity island-encoded type IV secretion system. [Internet] [Doctoral dissertation]. Vanderbilt University; 2011. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1803/14725.

Council of Science Editors:

Shaffer CL. Role of unique Helicobacter pylori proteins in the Cag pathogenicity island-encoded type IV secretion system. [Doctoral Dissertation]. Vanderbilt University; 2011. Available from: http://hdl.handle.net/1803/14725

6. Bashir, Ifat. Studies on the pathogenicity of microsporidia isolated from insect pests of mulberry and some other agricultural crops to the silkworm, Bombyx Mori L.; -.

Degree: Seri culture, 2008, University of Mysore

URL: http://shodhganga.inflibnet.ac.in/handle/10603/11139

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Microsporidiosis of silkworm is caused by a highly virulent parasiticmicrosporidian, Nosema bombycis. In addition to Nosema bombycis, several other strains and species of microsporidia have… (more)

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Microsporidiosis of silkworm is caused by a highly virulent parasiticmicrosporidian, Nosema bombycis. In addition to Nosema bombycis, several other strains and species of microsporidia have been isolated from the infected silkworms from time to time and the disease epizootic has become increasingly complex as more number of strains and species are being identified to be infecting silkworm. Microsporidia not only infect the silkworm, Bombyx mori L., but also different insect pests of mulberry and other agricultural crops. The infected insect pests of mulberry and also the insect pests from nearby agricultural crops that are casual visitors of mulberry gardens liberate the microsporidian spores along with their scales and faecal matter on the mulberry leaf and thereby contaminate the mulberry leaf. As a result, different microsporidia gain entry to silkworm rearing throu contaminated mulberry leaf and cross infect the silkworm. Hence, the present study was undertaken with an aim to investigate the various aspects of microsporidiosis in the silkworm, Bombyx mori L caused by microsporidia released from different insect pests on the mulberry eaves. In the present study, different insect pests of mulberry and other agricultural crops ere collected from mulberry gardens and agricultural crop fields in and around newlineMysore, Karnataka. The collected insects were screened for microsporid ian nfection and microsporidian spores were isolated from infected specimens. These spores were purified and their infectivity to silkworm was confirmed following Koch s postulates. The morphological characterization of the isolated microsporidia was carried out through light microscopy and micrometry and compared with that of the standard strain, Nosema bombycis. Scanning electron microscopic studies were also carried out to study the surface texture of the spores of the isolated microsporidia in comparison to that of the spores of Nosema bombycis.

References p. 214-243

Advisors/Committee Members: Sharma, S D.

Subjects/Keywords: agricultural crops; pathogenicity; microsporidia; silkworm

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APA (6^th Edition):

Bashir, I. (2008). Studies on the pathogenicity of microsporidia isolated from insect pests of mulberry and some other agricultural crops to the silkworm, Bombyx Mori L.; -. (Thesis). University of Mysore. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/11139

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bashir, Ifat. “Studies on the pathogenicity of microsporidia isolated from insect pests of mulberry and some other agricultural crops to the silkworm, Bombyx Mori L.; -.” 2008. Thesis, University of Mysore. Accessed March 03, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/11139.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bashir, Ifat. “Studies on the pathogenicity of microsporidia isolated from insect pests of mulberry and some other agricultural crops to the silkworm, Bombyx Mori L.; -.” 2008. Web. 03 Mar 2021.

Vancouver:

Bashir I. Studies on the pathogenicity of microsporidia isolated from insect pests of mulberry and some other agricultural crops to the silkworm, Bombyx Mori L.; -. [Internet] [Thesis]. University of Mysore; 2008. [cited 2021 Mar 03]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/11139.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bashir I. Studies on the pathogenicity of microsporidia isolated from insect pests of mulberry and some other agricultural crops to the silkworm, Bombyx Mori L.; -. [Thesis]. University of Mysore; 2008. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/11139

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Addis Ababa University

7. MIRTNEH, AKALU. ISOLATION AND MOLECULAR CHARACTERIZATION OF MAREK'S DISEASE VIRUS IN CENTRAL ETHIOPIA AND EVALUATION OF ITS VACCINE TRIAL .

Degree: 2015, Addis Ababa University

URL: http://etd.aau.edu.et/dspace/handle/123456789/7632

► The present study was conducted to isolate Marek's disease virus from outbreak samples and to evaluate HVT FC 126 vaccine strain against local isolates. The… (more)

▼ The present study was conducted to isolate Marek's disease virus from outbreak samples and to evaluate HVT FC 126 vaccine strain against local isolates. The outbreak investigation was performed in six purposively selected sites (Addis Ababa, Akaki, Dukem, Bishoftu, Mojo and Adama) on the basis of outbreak report. Complete random design was employed for pathogenicity and efficacy trial on Bovans brown chickens. A characteristics clinical signs of MD was observed on sick chickens. White foci of tumour on liver, heart and lung and enlargement of spleen were the post mortem findings of euthanized sick chickens. Based on vaccination history morbidity in non-vaccinated flocks ranges from 22.2% to 51% and mortality as high as 29%. In vaccinated flocks morbidity ranges from 0.7% to 3.3% and mortality as high as 0.4%. From 46/30 pooled samples collected 93.3% were confirmed to be MDV through virus specific genotyping methods, targeting the ICP4 gene of MD virus (318bp) using conventional PCR. 130 chickens were assigned in seven experimental groups to evaluate the pathgenicity of the six new cell culture-grown local isolates. Challenge was performed in two alternative doses (500 and 1000 pfu) by inoculating intraperitoneally at 8 day of age. Death was observed from 30% -50% and post mortem lesion 50%-70% at 1000 pfu. No death and post mortem lesion was observed from 60%-80% at 500 pfu. Thus, all six isolates at 1000 pfu dose fulfilled the criterion to be standard challenge strain. The evaluation of the efficacy of trial vaccine formulated from HVT FC 126 was performed on 70 chickens. Chickens immunized at day old through subcutaneous route with 2000 pfu were challenged in respective of the six local isolates at 7 day of post vaccination. None of the chickens were dead while only Addis Ababa, Dukem and Adama isolates caused tumour lesions in one chicken from their group. The actual MD incidence was rated from 0-10% in challenged groups. The protective index reaches to 95%. Thus the trial vaccine provided efficient protection against MD. However, the vaccine effectiveness shall be evaluated in the context of the existing poultry production system. Advisors/Committee Members: Dr. Bedasso Mamo (advisor).

Subjects/Keywords: Marek’s disease; pathogenicity; vaccine

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APA (6^th Edition):

MIRTNEH, A. (2015). ISOLATION AND MOLECULAR CHARACTERIZATION OF MAREK'S DISEASE VIRUS IN CENTRAL ETHIOPIA AND EVALUATION OF ITS VACCINE TRIAL . (Thesis). Addis Ababa University. Retrieved from http://etd.aau.edu.et/dspace/handle/123456789/7632

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

MIRTNEH, AKALU. “ISOLATION AND MOLECULAR CHARACTERIZATION OF MAREK'S DISEASE VIRUS IN CENTRAL ETHIOPIA AND EVALUATION OF ITS VACCINE TRIAL .” 2015. Thesis, Addis Ababa University. Accessed March 03, 2021. http://etd.aau.edu.et/dspace/handle/123456789/7632.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

MIRTNEH, AKALU. “ISOLATION AND MOLECULAR CHARACTERIZATION OF MAREK'S DISEASE VIRUS IN CENTRAL ETHIOPIA AND EVALUATION OF ITS VACCINE TRIAL .” 2015. Web. 03 Mar 2021.

Vancouver:

MIRTNEH A. ISOLATION AND MOLECULAR CHARACTERIZATION OF MAREK'S DISEASE VIRUS IN CENTRAL ETHIOPIA AND EVALUATION OF ITS VACCINE TRIAL . [Internet] [Thesis]. Addis Ababa University; 2015. [cited 2021 Mar 03]. Available from: http://etd.aau.edu.et/dspace/handle/123456789/7632.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

MIRTNEH A. ISOLATION AND MOLECULAR CHARACTERIZATION OF MAREK'S DISEASE VIRUS IN CENTRAL ETHIOPIA AND EVALUATION OF ITS VACCINE TRIAL . [Thesis]. Addis Ababa University; 2015. Available from: http://etd.aau.edu.et/dspace/handle/123456789/7632

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Guelph

8. de la Cerda, Karla Adriana. Morphological And Pathogenic Analyses Of Varieties Of Waitea Circinata And Their Rhizoctonia Anamorphs.

Degree: MS, Department of Environmental Biology, 2011, University of Guelph

URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3034

► The species complex, Waitea circinata (WC) has been currently divided into five cultural types: var. circinata, var. oryzae, var. zeae, var. agrostis, and var. prodigus.… (more)

Subjects/Keywords: Fungal; Rhizoctonia; Pathogenicity; Taxonomy; Phylogenetics

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APA (6^th Edition):

de la Cerda, K. A. (2011). Morphological And Pathogenic Analyses Of Varieties Of Waitea Circinata And Their Rhizoctonia Anamorphs. (Masters Thesis). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3034

Chicago Manual of Style (16^th Edition):

de la Cerda, Karla Adriana. “Morphological And Pathogenic Analyses Of Varieties Of Waitea Circinata And Their Rhizoctonia Anamorphs.” 2011. Masters Thesis, University of Guelph. Accessed March 03, 2021. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3034.

MLA Handbook (7^th Edition):

de la Cerda, Karla Adriana. “Morphological And Pathogenic Analyses Of Varieties Of Waitea Circinata And Their Rhizoctonia Anamorphs.” 2011. Web. 03 Mar 2021.

Vancouver:

de la Cerda KA. Morphological And Pathogenic Analyses Of Varieties Of Waitea Circinata And Their Rhizoctonia Anamorphs. [Internet] [Masters thesis]. University of Guelph; 2011. [cited 2021 Mar 03]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3034.

Council of Science Editors:

de la Cerda KA. Morphological And Pathogenic Analyses Of Varieties Of Waitea Circinata And Their Rhizoctonia Anamorphs. [Masters Thesis]. University of Guelph; 2011. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3034

University of Hawaii – Manoa

9. Srivastava, Shikha. Characterization and management of different Fusarium species associated with orchids cultivated in Hawaiʻi.

Degree: 2015, University of Hawaii – Manoa

URL: http://hdl.handle.net/10125/100471

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Ph.D. University of Hawaii at Manoa 2014.

Commercially grown orchids are among the most valuable ornamental crops produced in the United States. Hawaii is one… (more)

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Ph.D. University of Hawaii at Manoa 2014.

Commercially grown orchids are among the most valuable ornamental crops produced in the United States. Hawaii is one of the three largest orchids-growing states in the nation, producing the greatest variety of tropical species and unique intergeneric hybrids. A decrease in orchid production has been observed in Hawaii in association with spot and blights of shoots and flowers, as well as root rot that result in rapid death of juveniles and slow decline of mature plants. Such decline is now commonly observed in nearly all nurseries across the Oahu and the Island of Hawaii. Fungal pathogens are speculated to be one of the important sources of diseases in orchids in Hawaii. In the present study 16 fungal genera were isolated from 60 plant samples collected from Oahu and the Island of Hawaii. Fusarium was the most prevalent genus, with 78% of the isolates and frequently associated with several orchid cultivars, including over 9 genera and hybrids. Eleven Fusarium species, namely F. proliferatum, F. oxysporum, F. solani, F. subglutinans, F. poae, F. begoniae, F. bulbicola, F. anthophilum, F. denticulatum, F. circinatum and F. semitectum were found. Fusarium oxysporum was identified and was the most commonly isolated species from all the samples followed by F. proliferatum and F. solani. All Fusarium species were tested for pathogenicity on four orchid genera. Among the 11 Fusarium species, 6 were found pathogenic on 4 orchid genera. Three species, F. proliferatum, F. oxysporum and F. solani, showed moderate to high virulence on Dendrobium, Cymbidium and Miltonia. Fusarium circinatum and F. poae were low to moderate virulent on Dendrobium and Cymbidium whereas F. begoniae caused very low virulence on Dendrobium and Miltonia. Cattleya was susceptible to F. oxysporum only. Pathogenicity assays of Fusarium isolates on different cultivars of orchids resulted in identification of pathogenic Fusarium species was followed by testing efficacy of three fungicides, Pyraclostrobin, a.i. at 25%, Azoxystrobin a.i. at 50% and Triticonazole, a.i. at 20% on mycelial inhibition on four most prevalent Fusarium species namely-F. proliferatum, F. oxysporum, F. solani and F. subglutinans. Triticonazole was the most effective fungicide in the in vitro tests and was further evaluated for its efficacy in on Dendrobium plants in vivo against the most aggressive species of Fusarium, F. proliferatum under greenhouse conditions. This dissertation provides a thorough study to identify pathogenic Fusarium species on orchids and provides information regarding pathogenicity on orchid genera other than Dendrobium, and viable chemical methods to manage Fusarium species on orchids in Hawaii.

Subjects/Keywords: ornamental crops; pathogenicity; chemical methods

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APA (6^th Edition):

Srivastava, S. (2015). Characterization and management of different Fusarium species associated with orchids cultivated in Hawaiʻi. (Thesis). University of Hawaii – Manoa. Retrieved from http://hdl.handle.net/10125/100471

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Srivastava, Shikha. “Characterization and management of different Fusarium species associated with orchids cultivated in Hawaiʻi.” 2015. Thesis, University of Hawaii – Manoa. Accessed March 03, 2021. http://hdl.handle.net/10125/100471.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Srivastava, Shikha. “Characterization and management of different Fusarium species associated with orchids cultivated in Hawaiʻi.” 2015. Web. 03 Mar 2021.

Vancouver:

Srivastava S. Characterization and management of different Fusarium species associated with orchids cultivated in Hawaiʻi. [Internet] [Thesis]. University of Hawaii – Manoa; 2015. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10125/100471.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Srivastava S. Characterization and management of different Fusarium species associated with orchids cultivated in Hawaiʻi. [Thesis]. University of Hawaii – Manoa; 2015. Available from: http://hdl.handle.net/10125/100471

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

10. Massoumi, S. PMN inactivation of Candida albicans-MTT-reduction : a three hour model system suggesting that MTT reduction detects dead cells.

Degree: 1992, Oregon Health Sciences University

URL: doi:10.6083/M4PN93QN ; http://digitalcommons.ohsu.edu/etd/1767

Subjects/Keywords: Candida – pathogenicity; Phagocytosis

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APA (6^th Edition):

Massoumi, S. (1992). PMN inactivation of Candida albicans-MTT-reduction : a three hour model system suggesting that MTT reduction detects dead cells. (Thesis). Oregon Health Sciences University. Retrieved from doi:10.6083/M4PN93QN ; http://digitalcommons.ohsu.edu/etd/1767

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Massoumi, S. “PMN inactivation of Candida albicans-MTT-reduction : a three hour model system suggesting that MTT reduction detects dead cells.” 1992. Thesis, Oregon Health Sciences University. Accessed March 03, 2021. doi:10.6083/M4PN93QN ; http://digitalcommons.ohsu.edu/etd/1767.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Massoumi, S. “PMN inactivation of Candida albicans-MTT-reduction : a three hour model system suggesting that MTT reduction detects dead cells.” 1992. Web. 03 Mar 2021.

Vancouver:

Massoumi S. PMN inactivation of Candida albicans-MTT-reduction : a three hour model system suggesting that MTT reduction detects dead cells. [Internet] [Thesis]. Oregon Health Sciences University; 1992. [cited 2021 Mar 03]. Available from: doi:10.6083/M4PN93QN ; http://digitalcommons.ohsu.edu/etd/1767.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Massoumi S. PMN inactivation of Candida albicans-MTT-reduction : a three hour model system suggesting that MTT reduction detects dead cells. [Thesis]. Oregon Health Sciences University; 1992. Available from: doi:10.6083/M4PN93QN ; http://digitalcommons.ohsu.edu/etd/1767

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

11. Divya, Jose; Dr.Mohandas, A. Proteases from an Environmental Isolate of Pseudomonas Aeruginosa MCCB 123 and Their Applications.

Degree: 2011, Cochin University of Science and Technology

URL: http://dyuthi.cusat.ac.in/purl/2935

►

In the present study,heterotrophic protease producing bacterial isolates were screened for protease activity and a potent protease producing bacterial isolate was selected,identified and coded as… (more)

Subjects/Keywords: Proteases; Pathogenicity; Pseudomonas aeruginosa; Environmental Biotechnology

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APA (6^th Edition):

Divya, Jose; Dr.Mohandas, A. (2011). Proteases from an Environmental Isolate of Pseudomonas Aeruginosa MCCB 123 and Their Applications. (Thesis). Cochin University of Science and Technology. Retrieved from http://dyuthi.cusat.ac.in/purl/2935

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Divya, Jose; Dr.Mohandas, A. “Proteases from an Environmental Isolate of Pseudomonas Aeruginosa MCCB 123 and Their Applications.” 2011. Thesis, Cochin University of Science and Technology. Accessed March 03, 2021. http://dyuthi.cusat.ac.in/purl/2935.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Divya, Jose; Dr.Mohandas, A. “Proteases from an Environmental Isolate of Pseudomonas Aeruginosa MCCB 123 and Their Applications.” 2011. Web. 03 Mar 2021.

Vancouver:

Divya, Jose; Dr.Mohandas A. Proteases from an Environmental Isolate of Pseudomonas Aeruginosa MCCB 123 and Their Applications. [Internet] [Thesis]. Cochin University of Science and Technology; 2011. [cited 2021 Mar 03]. Available from: http://dyuthi.cusat.ac.in/purl/2935.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Divya, Jose; Dr.Mohandas A. Proteases from an Environmental Isolate of Pseudomonas Aeruginosa MCCB 123 and Their Applications. [Thesis]. Cochin University of Science and Technology; 2011. Available from: http://dyuthi.cusat.ac.in/purl/2935

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – San Diego

12. Ahn, Sae Jeong. Alterations in Pathogenicity Factors of Pseudomonas aeruginosa in Response to Cigarette Smoke Exposure.

Degree: Biology, 2016, University of California – San Diego

URL: http://www.escholarship.org/uc/item/94x6925t

► Cigarette smoking is one of the leading preventable causes of death in the United States. It is well recognized that cigarette smoking alters the human… (more)

Subjects/Keywords: Biology; Cigarette smoke; pathogenicity; Pseudomonas aeruginosa; virulence

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APA (6^th Edition):

Ahn, S. J. (2016). Alterations in Pathogenicity Factors of Pseudomonas aeruginosa in Response to Cigarette Smoke Exposure. (Thesis). University of California – San Diego. Retrieved from http://www.escholarship.org/uc/item/94x6925t

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ahn, Sae Jeong. “Alterations in Pathogenicity Factors of Pseudomonas aeruginosa in Response to Cigarette Smoke Exposure.” 2016. Thesis, University of California – San Diego. Accessed March 03, 2021. http://www.escholarship.org/uc/item/94x6925t.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ahn, Sae Jeong. “Alterations in Pathogenicity Factors of Pseudomonas aeruginosa in Response to Cigarette Smoke Exposure.” 2016. Web. 03 Mar 2021.

Vancouver:

Ahn SJ. Alterations in Pathogenicity Factors of Pseudomonas aeruginosa in Response to Cigarette Smoke Exposure. [Internet] [Thesis]. University of California – San Diego; 2016. [cited 2021 Mar 03]. Available from: http://www.escholarship.org/uc/item/94x6925t.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ahn SJ. Alterations in Pathogenicity Factors of Pseudomonas aeruginosa in Response to Cigarette Smoke Exposure. [Thesis]. University of California – San Diego; 2016. Available from: http://www.escholarship.org/uc/item/94x6925t

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Rochester

13. Bussey, Kendra Ann. Identification and Characterization of Polymerase Mutations That Contribute to Influenza Adaptation to Mammalian Hosts.

Degree: PhD, 2011, University of Rochester

URL: http://hdl.handle.net/1802/15954

► Influenza A viruses cause seasonal epidemics and occasional pandemics in humans. All 16 hemagglutinin and 9 neuraminidase subtypes have been detected in wild waterfowl, but… (more)

▼ Influenza A viruses cause seasonal epidemics and occasional pandemics in humans. All 16 hemagglutinin and 9 neuraminidase subtypes have been detected in wild waterfowl, but only limited strains can infect humans. The emergence of new influenza strains in humans can lead to pandemics, and this process may occur through two distinct mechanisms: reassortment of viral genes, or direct mutation. Viral reassortment of human, avian, and/or swine genes typically occurs in an intermediate swine host. The introduction of a new hemagglutinin or neuraminidase subtype to the human population can lead to the emergence of pandemics as occurred in the 1957, 1968, and 2009 influenza pandemics. Direct mutation of avian viruses allowing for human adaptation is an additional mechanism for the emergence of new viral strains in humans, as likely occurred in the 1918 pandemic. Direct infection of humans with highly pathogenic H5N1 avian viruses is possible and has occurred in Southeast Asia, the Middle East, and Africa. The mutations required for host adaptation are found in multiple viral genes, including the hemagglutinin and the heterotrimeric RNA-dependent RNA polymerase comprised of PA, PB1, and PB2. Previous studies have revealed a single mutation in PB2, E627K, significantly enhances polymerase activity and pathogenicity in the mammalian host. Conversely, avian polymerases that contain PB2 627E typically result in low activity and pathogenicity in mammalian cells and hosts. However, some highly pathogenic H5N1 avian influenza viruses lack PB2 627K, but cause severe disease in mammals. The mechanism of adaptation of these highly pathogenic PB2 627E viruses is unknown. We hypothesized that additional, unidentified changes in the polymerase complex can contribute to enhanced polymerase activity and pathogenicity of avian influenza strains in mammalian species. To identify the components and mutations responsible for polymerase activity in the mammalian host, we analyzed the activity of human and avian influenza polymerase complexes using a minigenome polymerase activity assay. In avian DF-1 cells, polymerase complexes from the human strain A/Aichi/2/68 (Aichi, H3N2) and avian strain A/chicken/Nanchang/3-120/01 (Nan, H3N2) were highly active. In human 293T cells, however, the Aichi complex was highly active, while the activity of the avian Nan complex was very low. Single-gene replacement experiments revealed that the avian Nan activity defect in mammalian cells was due to the avian PB2 gene, because replacement with the human Aichi PB2 significantly increased Nan polymerase activity in mammalian 293T cells. To identify amino acids responsible for this phenotype, we compared human and avian PB2 sequences, and found 10 highly conserved residues specific to either human or avian influenza strains. We introduced human conserved residues into the avian Nan PB2, and analyzed their contribution to polymerase activity. As expected, the mutation E627K significantly increased Nan polymerase activity in 293T cells. Additionally, the avian to…

Subjects/Keywords: Influenza; Polymerase; Host Adaptation; PB2; PA; Pathogenicity

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APA (6^th Edition):

Bussey, K. A. (2011). Identification and Characterization of Polymerase Mutations That Contribute to Influenza Adaptation to Mammalian Hosts. (Doctoral Dissertation). University of Rochester. Retrieved from http://hdl.handle.net/1802/15954

Chicago Manual of Style (16^th Edition):

Bussey, Kendra Ann. “Identification and Characterization of Polymerase Mutations That Contribute to Influenza Adaptation to Mammalian Hosts.” 2011. Doctoral Dissertation, University of Rochester. Accessed March 03, 2021. http://hdl.handle.net/1802/15954.

MLA Handbook (7^th Edition):

Bussey, Kendra Ann. “Identification and Characterization of Polymerase Mutations That Contribute to Influenza Adaptation to Mammalian Hosts.” 2011. Web. 03 Mar 2021.

Vancouver:

Bussey KA. Identification and Characterization of Polymerase Mutations That Contribute to Influenza Adaptation to Mammalian Hosts. [Internet] [Doctoral dissertation]. University of Rochester; 2011. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1802/15954.

Council of Science Editors:

Bussey KA. Identification and Characterization of Polymerase Mutations That Contribute to Influenza Adaptation to Mammalian Hosts. [Doctoral Dissertation]. University of Rochester; 2011. Available from: http://hdl.handle.net/1802/15954

Universiteit Utrecht

14. Ankum, L. Presence and pathogenicity of Paramphistomidae in ruminants in The Netherlands.

Degree: 2015, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/306065

► A study has been performed to get an impression about the presence and pathogenicity of rumen fluke in ruminants in the Netherlands. The study included… (more)

Subjects/Keywords: Rumen fluke; paramhistomidae; prevalence; presence; pathogenicity

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APA (6^th Edition):

Ankum, L. (2015). Presence and pathogenicity of Paramphistomidae in ruminants in The Netherlands. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/306065

Chicago Manual of Style (16^th Edition):

Ankum, L. “Presence and pathogenicity of Paramphistomidae in ruminants in The Netherlands.” 2015. Masters Thesis, Universiteit Utrecht. Accessed March 03, 2021. http://dspace.library.uu.nl:8080/handle/1874/306065.

MLA Handbook (7^th Edition):

Ankum, L. “Presence and pathogenicity of Paramphistomidae in ruminants in The Netherlands.” 2015. Web. 03 Mar 2021.

Vancouver:

Ankum L. Presence and pathogenicity of Paramphistomidae in ruminants in The Netherlands. [Internet] [Masters thesis]. Universiteit Utrecht; 2015. [cited 2021 Mar 03]. Available from: http://dspace.library.uu.nl:8080/handle/1874/306065.

Council of Science Editors:

Ankum L. Presence and pathogenicity of Paramphistomidae in ruminants in The Netherlands. [Masters Thesis]. Universiteit Utrecht; 2015. Available from: http://dspace.library.uu.nl:8080/handle/1874/306065

University of Guelph

15. Grgic, Helena. Sequence analysis, pathogenicity and cytokine gene expression patterns associated with fowl adenovirus infection.

Degree: PhD, Department of Pathobiology, 2012, University of Guelph

URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3644

► The family Adenoviridae consists of five genera, including the genus Aviadenovirus, which infects avian species. The genus Aviadenovirus currently comprises five fowl (Fowl adenovirus A-E),… (more)

▼ The family Adenoviridae consists of five genera, including the genus Aviadenovirus, which infects avian species. The genus Aviadenovirus currently comprises five fowl (Fowl adenovirus A-E), one falcon (Falcon adenovirus A), and one goose (Goose adenovirus) adenovirus species. Fowl adenoviruses (FAdVs) have a worldwide distribution. Some are associated with diseases such as inclusion body hepatitis (IBH), while FAdV species C serotype 4 (FAdV-4) has been associated with hydropericardium-hepatitis syndrome (HHS). In this study, the complete nucleotide sequence of fowl adenovirus serotype 8 (FAdV-8) was determined. The full genome was 44,055 nucleotides (nt) in length, with an organization similar to that of the FAdV-1 and FAdV-9 genomes. No regions homologous to early regions E1, E3, and E4 of mastadenoviruses were recognized Pathogenicity of FAdV-8 and FAdV-4 were studied in specific-pathogen-free chickens following oral and intramuscular inoculations. Pathogenicity was determined on the basis of clinical signs and gross and histological lesions. Additionally, virus shedding and viral genome copy numbers in liver, cecal tonsil, and bursa of Fabricius were determined. The role of interleukins (IL) in the pathogenicity of and immune response to FAdVs is unknown. Therefore, in a chicken experiment, interferon-γ, IL-10, IL-18, and IL-8 gene expression was evaluated following FAdV-8 and FAdV-4 infection. Cytokine gene expression was examined in the liver, spleen, and cecal tonsils. This study explored the ability of fowl adenoviruses to subvert the host cell’s secretion of cytokines in response to infection as an important viral mechanism for immune evasion during infection. Variations in virulence of FAdVs are likely to be determined by the fiber alone as shown by Pallister et al. (1996). Therefore, we compared and analyzed the nt and amino acid (aa) sequences of the fiber gene of pathogenic and non-pathogenic FAdVs representing species groups D (FAdV-11) and E (FAdV-8). According to our data, virulence might not be associated only with sequence of the fiber gene. This work is a continuation of our efforts towards better understanding of the molecular biology of FAdVs and the pathogenesis of the disease, with an emphasis on the role of interleukins, an unknown area. Advisors/Committee Members: Nagy, Eva (advisor).

Subjects/Keywords: Sequence analysis; pathogenicity; cytokine gene expression patterns

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APA (6^th Edition):

Grgic, H. (2012). Sequence analysis, pathogenicity and cytokine gene expression patterns associated with fowl adenovirus infection. (Doctoral Dissertation). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3644

Chicago Manual of Style (16^th Edition):

Grgic, Helena. “Sequence analysis, pathogenicity and cytokine gene expression patterns associated with fowl adenovirus infection.” 2012. Doctoral Dissertation, University of Guelph. Accessed March 03, 2021. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3644.

MLA Handbook (7^th Edition):

Grgic, Helena. “Sequence analysis, pathogenicity and cytokine gene expression patterns associated with fowl adenovirus infection.” 2012. Web. 03 Mar 2021.

Vancouver:

Grgic H. Sequence analysis, pathogenicity and cytokine gene expression patterns associated with fowl adenovirus infection. [Internet] [Doctoral dissertation]. University of Guelph; 2012. [cited 2021 Mar 03]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3644.

Council of Science Editors:

Grgic H. Sequence analysis, pathogenicity and cytokine gene expression patterns associated with fowl adenovirus infection. [Doctoral Dissertation]. University of Guelph; 2012. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3644

University of Pretoria

16. Mandyoli, Lungelo. Structural characterization of EtpA an adhesin from enterotoxigenic Escherichia coli (ETEC).

Degree: MSc, Biochemistry, 2016, University of Pretoria

URL: http://hdl.handle.net/2263/60835

► Enterotoxigenic Escherichia coli (ETEC) encompass a group of diverse bacterial pathogens that collectively cause hundreds of millions of diarrheal cases annually, mostly in developing countries.… (more)

▼ Enterotoxigenic Escherichia coli (ETEC) encompass a group of diverse bacterial pathogens that collectively cause hundreds of millions of diarrheal cases annually, mostly in developing countries. As part of its infection strategy, ETEC invades and colonizes small intestinal epithelial cells where it secretes heat-labile and/or heat-stable enterotoxins, inducing diarrhoea. The ability of ETEC to invade human epithelial cells is a hallmark of its pathogenicity and is controlled by a set of plasmid and chromosome encoded virulence factors. They include EtpA, a 170 kDa plasmid encoded autotransporter. During infection, EtpA functions as an adhesin linking flagellin at the tip of ETEC flagella to the host cell surface and allowing ETEC to deposit its toxins. Antibodies targeting either EtpA or the conserved regions of flagellin impair delivery of the heat-labile toxin in vitro, and prevent intestinal colonization of mice following gastrointestinal challenge with ETEC. EtpA is thus critical to the pathogenicity of ETEC. In this study, a truncated version of EtpA (35 kDa) termed N-terminal EtpA69-445 or N-EtpA69-445 was cloned and produced as an N-terminal GST-tagged cytoplasmic fusion protein in E. coli BL21 cells. The protein was purified by affinity chromatography on glutathione agarose beads. However, the yield of the pure protein was poor due to its limited solubility. As an alternative, a 57 kDa truncated version of EtpA (N-EtpA69-607) was produced as a secreted C-terminal His6-tagged fusion protein in E. coli TOP10 cells. The protein was purified to homogeneity by metal affinity chromatography (MAC) using Ni-NTA and ion exchange chromatography (IEC) on a Mono S 10/100 GL column. Biophysical characterization of N-EtpA69-607 using circular dichroism (CD) spectroscopy revealed the typical spectrum of a β-helical protein. The in silico modelled structure of the protein confirmed N-EtpA69-607 to be a β-helical protein. CD spectra recorded at increasing temperatures indicated N-EtpA69-607 to be thermally highly stable retaining its fold up to 95°C. Dynamic light scattering (DLS) experiments showed that N-EtpA69-607 is polydisperse in solution forming higher oligomers. Lead crystallization conditions of N- EtpA69-607 were determined but the crystals were too small for X-ray data collection. This study thus represents a significant step towards the characterization of the three dimensional structure of EtpA and understanding its structure-function relationship. Advisors/Committee Members: Schubert, Wolf-Dieter (advisor), Motshwene, Precious, G. (coadvisor).

Subjects/Keywords: UCTD; Autotransporter; Protein secretion; Pathogenicity; Crystallization

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APA (6^th Edition):

Mandyoli, L. (2016). Structural characterization of EtpA an adhesin from enterotoxigenic Escherichia coli (ETEC). (Masters Thesis). University of Pretoria. Retrieved from http://hdl.handle.net/2263/60835

Chicago Manual of Style (16^th Edition):

Mandyoli, Lungelo. “Structural characterization of EtpA an adhesin from enterotoxigenic Escherichia coli (ETEC).” 2016. Masters Thesis, University of Pretoria. Accessed March 03, 2021. http://hdl.handle.net/2263/60835.

MLA Handbook (7^th Edition):

Mandyoli, Lungelo. “Structural characterization of EtpA an adhesin from enterotoxigenic Escherichia coli (ETEC).” 2016. Web. 03 Mar 2021.

Vancouver:

Mandyoli L. Structural characterization of EtpA an adhesin from enterotoxigenic Escherichia coli (ETEC). [Internet] [Masters thesis]. University of Pretoria; 2016. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/2263/60835.

Council of Science Editors:

Mandyoli L. Structural characterization of EtpA an adhesin from enterotoxigenic Escherichia coli (ETEC). [Masters Thesis]. University of Pretoria; 2016. Available from: http://hdl.handle.net/2263/60835

University of Manitoba

17. Zhu, Xiaohan. Identification and characterization of pathogenicity-related factors in Verticillium dahliae.

Degree: Plant Science, 2019, University of Manitoba

URL: http://hdl.handle.net/1993/34304

► Verticillium dahliae is the main causal agent of potato early dying (PED) disease in Canada and can lead to yield losses of up to 50%.… (more)

▼ Verticillium dahliae is the main causal agent of potato early dying (PED) disease in Canada and can lead to yield losses of up to 50%. The management of this disease is usually costly and inefficient. Therefore, it is important to understand the molecular interaction between the host and pathogen, in order to determine the key components leading to disease or resistance. Dr. Daayf’s lab previously identified several proteins and/or genes showing differentiatial activity between highly and weakly aggressive V. dahliae isolates. Isochorismatase Hydrolase, NADPH oxidases, exopolygalacturonase, Ras-GAP like protein, serine/threonine-protein kinase, Ubiquitin-conjugating enzyme variant MMS2, NADH-ubiquinone oxidoreductase, Thioredoxin, Pyruvate dehydrogenase E1 component subunit beta, myo-inositol 2-dehydrogenase, and HAD-superfamily hydrolases all showed higher activity in the highly aggressive isolate compared to the weakly aggressive one. We hypothesized that Isochorismatase Hydrolase (VdICSH1) of V. dahliae may interfere with the SA synthesis pathway of potato during their interaction. We generated gene disruption mutants for VdICSH1 in the highly aggressive isolate, and showed their reduced virulence compared with the wild type. Plant SA and JA accumulation showed different tendencies in the stems and roots at early infection stages, and similar tendencies in the leaves at later stages, when exposed to V. dahliae infection. It was also apparent that other isochorismatase family members in the icsh1 mutants compensate for the absence of ICSH1. Further, we investigated the function of ROS generated by NADPH oxidases in V. dahliae, by generating mutants for NoxA, NoxB and NoxC in the highly aggressive isolate. The noxa and noxb mutants lost their penetration ability and could not form penetration pegs after 72h on cellophane membrane, which led to a significant decrease in their virulence on potato. Finally, we employed a new vector–free split-marker recombination method, derived from Saccharomyces cerevisiae, to quickly obtain knock-out mutants for the exopolygalacturonase (ExoPG) gene in the highly aggressive V. dahliae isolate. This gene showed more transcriptional activity in response to leaf and stem extracts, as well as during early infection stage. Advisors/Committee Members: Daayf, Fouad (Plant Science) (supervisor), Rampitsch, Christof (Plant Science) (examiningcommittee), Li, Genyi (Plant Science) (examiningcommittee), Hausner, Georg (Microbiology) (examiningcommittee), Bernier, Louis (Laval University) (examiningcommittee).

Subjects/Keywords: Verticillium dahliae; Pathogenicity; ICSH1; NoxA; NoxB; ExoPG

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APA (6^th Edition):

Zhu, X. (2019). Identification and characterization of pathogenicity-related factors in Verticillium dahliae. (Thesis). University of Manitoba. Retrieved from http://hdl.handle.net/1993/34304

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zhu, Xiaohan. “Identification and characterization of pathogenicity-related factors in Verticillium dahliae.” 2019. Thesis, University of Manitoba. Accessed March 03, 2021. http://hdl.handle.net/1993/34304.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zhu, Xiaohan. “Identification and characterization of pathogenicity-related factors in Verticillium dahliae.” 2019. Web. 03 Mar 2021.

Vancouver:

Zhu X. Identification and characterization of pathogenicity-related factors in Verticillium dahliae. [Internet] [Thesis]. University of Manitoba; 2019. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1993/34304.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zhu X. Identification and characterization of pathogenicity-related factors in Verticillium dahliae. [Thesis]. University of Manitoba; 2019. Available from: http://hdl.handle.net/1993/34304

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Florida Atlantic University

18. Regmi, Deepika. INVESTIGATING THE AMYLOIDOGENESIS OF A PRION PEPTIDE (106-128).

Degree: MS, 2020, Florida Atlantic University

URL: http://fau.digital.flvc.org/islandora/object/fau:44447

►

The misfolding of native, cellular prion protein (PrPc) to a conformationally altered pathogenic isoform, designated scrapie PrPsc, is the main molecular process involved in the… (more)

▼

The misfolding of native, cellular prion protein (PrPc) to a conformationally altered pathogenic isoform, designated scrapie PrPsc, is the main molecular process involved in the pathogenesis of prion diseases. Prion diseases are marked by the accumulation of conformationally modified forms of cellular prion protein. An N-terminal portion of the prion protein, PrP (106-128), is a 23-residue peptide fragment and is characterized by an amphipathic structure with two domains: a hydrophilic N-terminal domain and a hydrophobic C-terminal domain. In this study, the aggregation characteristics of the PrP (106-128) peptide were investigated using a combination of biophysical approaches. We investigated the effect of different factors including concentrations, pH, and metal ions, on the aggregation of the peptide. Our results demonstrated that the peptide steadily aggregates at concentrations higher than 25 M. The aggregation propensity and fibril formation is higher at pH 7.4 and pH 8.1, and the aggregation is inhibited at pH lower than 6. Furthermore, our results indicate that the Cu2+ has much less effect on the peptide amyloidogenesis, while Zn2+ has a significant influence on the PrP (106-128) amyloidogenesis. We further presented a systematic analysis of the impact of phospholipid liposomes of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1’-racglycerol) (POPG) in the absence or presence of cholesterol, on the amyloidogenesis of PrP (106-128). The results showed that POPC vesicles does not significantly influence the aggregation kinetics of the peptide. However, the anionic lipid POPG delays the aggregation in a concentration-dependent manner, whereas the addition of POPG with the cholesterol shows fast kinetics of fibrillization, thus reducing the lag time of the aggregation kinetics. We also monitored the effect of cholesterol and its derivatives including cholesterol-SO4 and DC-cholesterol on PrP (106-128) amyloidogenesis. Our results showed that the cholesterol inhibits the peptide aggregation and delays the formation of fibrils in a concentration-dependent manner. Cholesterol-SO4 dramatically facilitates the aggregation at high concentrations but has the potential to slow down the fibrillization at low concentrations, whereas cationic DC-cholesterol vesicles can effectively inhibit peptide fibril formation at high concentrations.

2020

Degree granted:

Collection: FAU

Advisors/Committee Members: Du, Deguo (Thesis advisor), Florida Atlantic University (Degree grantor), Department of Chemistry and Biochemistry, Charles E. Schmidt College of Science.

Subjects/Keywords: Prion Diseases; Prions – pathogenicity; Amyloid; Peptides; Prions

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Regmi, D. (2020). INVESTIGATING THE AMYLOIDOGENESIS OF A PRION PEPTIDE (106-128). (Masters Thesis). Florida Atlantic University. Retrieved from http://fau.digital.flvc.org/islandora/object/fau:44447

Chicago Manual of Style (16^th Edition):

Regmi, Deepika. “INVESTIGATING THE AMYLOIDOGENESIS OF A PRION PEPTIDE (106-128).” 2020. Masters Thesis, Florida Atlantic University. Accessed March 03, 2021. http://fau.digital.flvc.org/islandora/object/fau:44447.

MLA Handbook (7^th Edition):

Regmi, Deepika. “INVESTIGATING THE AMYLOIDOGENESIS OF A PRION PEPTIDE (106-128).” 2020. Web. 03 Mar 2021.

Vancouver:

Regmi D. INVESTIGATING THE AMYLOIDOGENESIS OF A PRION PEPTIDE (106-128). [Internet] [Masters thesis]. Florida Atlantic University; 2020. [cited 2021 Mar 03]. Available from: http://fau.digital.flvc.org/islandora/object/fau:44447.

Council of Science Editors:

Regmi D. INVESTIGATING THE AMYLOIDOGENESIS OF A PRION PEPTIDE (106-128). [Masters Thesis]. Florida Atlantic University; 2020. Available from: http://fau.digital.flvc.org/islandora/object/fau:44447

University of Melbourne

19. Butler, Jeffrey Michael. Avian influenza: a study of molecular pathogenesis.

Degree: 2012, University of Melbourne

URL: http://hdl.handle.net/11343/38000

► Since 1997 over 600 people have been infected with haemagglutinin subtype 5-neuraminidase subtype 1 (H5N1) highly pathogenic avian influenza (HPAI) viruses (herein referred to as… (more)

▼ Since 1997 over 600 people have been infected with haemagglutinin subtype 5-neuraminidase subtype 1 (H5N1) highly pathogenic avian influenza (HPAI) viruses (herein referred to as H5N1), of which approximately 60% have died. Amongst the cases of human H5N1 infection a wide spectrum of clinical disease symptoms have been observed. While some humans have only experienced a mild influenza-like-illness, others have experienced severe pneumonia, respiratory distress, multiple organ dysfunction and an inevitably fatal outcome. Given these differences, this project sets out to investigate whether H5N1 viruses undergo a process of adaptation following the infection of a mammal directly from an avian host. For this purpose, ferrets were inoculated with the avian isolate of H5N1 virus A/Chicken/Laos/Xaythiani-26/2006 (A/Laos). Although this virus was 100% lethal in poultry it only caused a mild disease in ferrets early after infection. However, two of four ferrets inoculated with this virus, that were sampled at later time points post inoculation (pi), developed weight loss and a systemic disease. Sequence analysis of A/Laos recovered from different tissues of both these ferrets, revealed the presence of the same amino acid (aa) substitution from glutamate (E) to lysine (K) at position 627 of the viral polymerase basic-2 (PB2) protein (herein referred to as PB2 627), an aa substitution that had previously been implicated as a host adaptation of avian influenza viruses to humans. Virus recovered from each of the A/Laos-infected ferrets that developed weight loss and systemic disease, was subsequently reinoculated into further ferrets. In each group of ferrets inoculated with a ferret passaged A/Laos isolate encoding PB2 627 K, substantially increased virus pathogenicity was observed. In addition, enhanced pathogenicity was also observed in a group of ferrets inoculated with an A/Laos isolate that had retained PB2 627 E, yet had acquired aa substitutions from serine (S) to proline (P) at position 489 of the PB2 protein as well as valine (V) to isoleucine (I) at position 408 of the nucleoprotein (NP). Finally, a reverse genetics (rg) system was utilised to generate isogenic H5N1 viruses encoding single aa changes at positions 627 and 489 of the PB2 protein, and position 408 of the NP. Inoculation of ferrets with each of these viruses confirmed that the PB2 627 E→K aa substitution dramatically enhanced the pathogenicity of H5N1 in ferrets. In contrast, ferrets inoculated with A/Laos encoding the PB2 489 S→P aa substitution displayed only marginally enhanced disease symptoms, while the NP 408 V→I aa substitution had no effect on virus pathogenicity, suggesting that these two aa changes only facilitate enhanced virus pathogenicity when they are both present within the same virus. The experiments in ferrets presented in this thesis suggest that H5N1 viruses…

Subjects/Keywords: Avian influenza H5N1 pathogenicity polmerase mammals

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Butler, J. M. (2012). Avian influenza: a study of molecular pathogenesis. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/38000

Chicago Manual of Style (16^th Edition):

Butler, Jeffrey Michael. “Avian influenza: a study of molecular pathogenesis.” 2012. Doctoral Dissertation, University of Melbourne. Accessed March 03, 2021. http://hdl.handle.net/11343/38000.

MLA Handbook (7^th Edition):

Butler, Jeffrey Michael. “Avian influenza: a study of molecular pathogenesis.” 2012. Web. 03 Mar 2021.

Vancouver:

Butler JM. Avian influenza: a study of molecular pathogenesis. [Internet] [Doctoral dissertation]. University of Melbourne; 2012. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/11343/38000.

Council of Science Editors:

Butler JM. Avian influenza: a study of molecular pathogenesis. [Doctoral Dissertation]. University of Melbourne; 2012. Available from: http://hdl.handle.net/11343/38000

Louisiana State University

20. Guan, Dongli. Insights into the Molecular Mechanism of Streptomyces ipomoeae Pathogenesis: Thaxtomin C Biosynthesis and Regulation.

Degree: PhD, 2011, Louisiana State University

URL: etd-04192011-184316 ; https://digitalcommons.lsu.edu/gradschool_dissertations/445

► Streptomyces ipomoeae is the causal agent of Streptomyces soil rot of sweetpotato, a disease marked by highly necrotic destruction of adventitious roots, including the development… (more)

▼ Streptomyces ipomoeae is the causal agent of Streptomyces soil rot of sweetpotato, a disease marked by highly necrotic destruction of adventitious roots, including the development of necrotic lesions on the fleshy storage roots. To facilitate further genetic manipulation of this agriculturally important pathogen, an effective gene transfer system for S. ipomoeae was developed by using an optimized intergeneric conjugation procedure from Escherichia coli. Conjugation results varied greatly depending on the particular media used for S. ipomoeae spore preparation and conjugation. Transconjugant to recipient ratios as great as 4.1 x 10-5 were achieved when International Streptomyces Project Medium 4 was used for both sporulation and conjugation protocols. Both site-specifically integrating and autonomously replicating plasmids could be introduced and maintained in S. ipomoeae, and plasmids could be introduced with approximate equivalent frequencies from either methyl-proficient or methyl-deficient E. coli donors; the latter result indicates a likely absence of relevant methyl-specific restriction in S. ipomoeae. Streptomyces potato scab pathogens produce a phytotoxin (thaxtomin A) that appears to facilitate their entrance into host plants. S. ipomoeae produces a less-modified thaxtomin derivative (thaxtomin C) whose role in pathogenicity has not been examined. Here, we cloned and sequenced the thaxtomin C (txt) gene cluster of S. ipomoeae and I then constructed targeted txt mutants. The mutants were unable to penetrate intact adventitious roots but still caused necrosis on storage root tissue. These results, taken in context with previous histopathological study of S. ipomoeae infection, suggest that thaxtomin C plays an essential role in inter- and intracellular penetration of sweetpotato adventitious roots by S. ipomoeae. Once inside the plant host, the pathogen uses a yet-to-be-determined factor(s) to necrotize root tissue, including that of any storage roots it encounters. An AraC/XylS family transcriptional regulator TxtR encoded in the thaxtomin C gene cluster is shown here to be essential for S. ipomoeae pathogenicity, though the putative host-derived ligand that binds TxtR to activate thaxtomin C production remains to be identified. Another conserved gene txtH, which is also imbedded in the thaxtomin C gene cluster, was found to be not required for S. ipomoeae pathogenicity.

Subjects/Keywords: pathogenicity; soil rot; thaxtomin; Streptomyces ipomoeae

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Guan, D. (2011). Insights into the Molecular Mechanism of Streptomyces ipomoeae Pathogenesis: Thaxtomin C Biosynthesis and Regulation. (Doctoral Dissertation). Louisiana State University. Retrieved from etd-04192011-184316 ; https://digitalcommons.lsu.edu/gradschool_dissertations/445

Chicago Manual of Style (16^th Edition):

Guan, Dongli. “Insights into the Molecular Mechanism of Streptomyces ipomoeae Pathogenesis: Thaxtomin C Biosynthesis and Regulation.” 2011. Doctoral Dissertation, Louisiana State University. Accessed March 03, 2021. etd-04192011-184316 ; https://digitalcommons.lsu.edu/gradschool_dissertations/445.

MLA Handbook (7^th Edition):

Guan, Dongli. “Insights into the Molecular Mechanism of Streptomyces ipomoeae Pathogenesis: Thaxtomin C Biosynthesis and Regulation.” 2011. Web. 03 Mar 2021.

Vancouver:

Guan D. Insights into the Molecular Mechanism of Streptomyces ipomoeae Pathogenesis: Thaxtomin C Biosynthesis and Regulation. [Internet] [Doctoral dissertation]. Louisiana State University; 2011. [cited 2021 Mar 03]. Available from: etd-04192011-184316 ; https://digitalcommons.lsu.edu/gradschool_dissertations/445.

Council of Science Editors:

Guan D. Insights into the Molecular Mechanism of Streptomyces ipomoeae Pathogenesis: Thaxtomin C Biosynthesis and Regulation. [Doctoral Dissertation]. Louisiana State University; 2011. Available from: etd-04192011-184316 ; https://digitalcommons.lsu.edu/gradschool_dissertations/445

University of Georgia

21. Dlugolenski, Daniel Aaron. Understanding features contributing to species-specific influenza virus reassortment and identification of novel influenza A virus pathogenicity factors.

Degree: 2016, University of Georgia

URL: http://hdl.handle.net/10724/35147

► The segmented genome of Influenza A viruses (IAV) allows for genetic exchange resulting in evolutionary shift. The 2009 pandemic was a result of a triple… (more)

Subjects/Keywords: Influenza; reassortment; pathogenicity; acute lung injury

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dlugolenski, D. A. (2016). Understanding features contributing to species-specific influenza virus reassortment and identification of novel influenza A virus pathogenicity factors. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/35147

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dlugolenski, Daniel Aaron. “Understanding features contributing to species-specific influenza virus reassortment and identification of novel influenza A virus pathogenicity factors.” 2016. Thesis, University of Georgia. Accessed March 03, 2021. http://hdl.handle.net/10724/35147.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dlugolenski, Daniel Aaron. “Understanding features contributing to species-specific influenza virus reassortment and identification of novel influenza A virus pathogenicity factors.” 2016. Web. 03 Mar 2021.

Vancouver:

Dlugolenski DA. Understanding features contributing to species-specific influenza virus reassortment and identification of novel influenza A virus pathogenicity factors. [Internet] [Thesis]. University of Georgia; 2016. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10724/35147.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dlugolenski DA. Understanding features contributing to species-specific influenza virus reassortment and identification of novel influenza A virus pathogenicity factors. [Thesis]. University of Georgia; 2016. Available from: http://hdl.handle.net/10724/35147

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Georgia

22. Nadal, Marina. Exploring the role of autophagy and cell wall degrading enzymes in the life cycle and pathogenic development of the basidiomycete fungal plant pathogen Ustilago maydis.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/26111

► The plant pathogen Ustilago maydis, of the Basidiomycota is responsible for corn smut disease and an important model organism for this fungal phylum. One of… (more)

▼ The plant pathogen Ustilago maydis, of the Basidiomycota is responsible for corn smut disease and an important model organism for this fungal phylum. One of the main aims in our laboratory is to investigate the genetic determinants involved in the processes associated with U. maydis morphogenesis and pathogenic development. In the work reported here, I was particularly interested in addressing the roles of autophagy and the regulation of cell wall degrading enzymes (CWDE) as key controlling factors of U. maydis development and virulence. Using a reverse genetic approach and a combination of other techniques, we firstly showed here that the U. maydis autophagy related genes atg1 and atg8, are associated with autophagy dependent processes. Deletion of atg8 resulted in a lower survival capacity during carbon starvation conditions, abnormal morphogenesis, and most importantly severe reduction of virulence. Interestingly, Δatg1 strains yielded phenotypes similar to the Δatg8 strains but of lower magnitude. Surprisingly, atg1 deletion in the Δatg8 background generated an additive phenotype. We then focused on the study of U. maydis CWDE genes as potential virulence factors regulated by carbon source availability. Little is known about the mode that U. maydis employs to penetrate and spread in the corn plant and CWDE involvement in these processes has been hypothesized but never thoroughly tested. CWDE genes in fungi are often subject to transcriptional glucose repression. In yeast, one of the main players controlling this process is SNF1, which encodes a protein kinase. SNF1 homologue disruption in the phytopathogenic fungi Fusarium oxysporum and Cochliobolus carbonum led to a reduction in the expression of several CWDE genes accompanied by a decrease in virulence. In this work we showed that in U. maydis Snf1 acts as either a negative or positive regulator of particular CWDE genes and is not required for metabolism of alternative carbon sources. Unlike in Ascomycete plant pathogens, deletion of snf1 did not profoundly affect virulence in U. maydis.

Subjects/Keywords: autophagy; pathogenicity; budding; autophagosomes; galls; teliospores; nutrient stress; CWDE; glucose repression; pathogenicity; carbon utilization; morphogensis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nadal, M. (2014). Exploring the role of autophagy and cell wall degrading enzymes in the life cycle and pathogenic development of the basidiomycete fungal plant pathogen Ustilago maydis. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/26111

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Nadal, Marina. “Exploring the role of autophagy and cell wall degrading enzymes in the life cycle and pathogenic development of the basidiomycete fungal plant pathogen Ustilago maydis.” 2014. Thesis, University of Georgia. Accessed March 03, 2021. http://hdl.handle.net/10724/26111.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Nadal, Marina. “Exploring the role of autophagy and cell wall degrading enzymes in the life cycle and pathogenic development of the basidiomycete fungal plant pathogen Ustilago maydis.” 2014. Web. 03 Mar 2021.

Vancouver:

Nadal M. Exploring the role of autophagy and cell wall degrading enzymes in the life cycle and pathogenic development of the basidiomycete fungal plant pathogen Ustilago maydis. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10724/26111.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nadal M. Exploring the role of autophagy and cell wall degrading enzymes in the life cycle and pathogenic development of the basidiomycete fungal plant pathogen Ustilago maydis. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/26111

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

23. Botelho, Luana da Silva. Fungos associados às sementes de ipê-amarelo (Tabebuia serratifolia), ipê-roxo (Tabebuia impetiginosa), aroeira-pimenteira (Schinus terebinthifolius) e aroeira-salsa (Schinus molle): incidência, efeitos na germinação, transmissão para plântulas e controle.

Degree: Mestrado, Microbiologia Agrícola, 2007, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/11/11138/tde-08032007-142028/ ;

► Os objetivos deste trabalho foram detectar e identificar os fungos presentes em amostras de sementes de ipê-amarelo (Tabebuia serratifolia), ipê-roxo (Tabebuia impetiginosa), aroeira-pimenteira (Schinus terebinthifolius)… (more)

▼ Os objetivos deste trabalho foram detectar e identificar os fungos presentes em amostras de sementes de ipê-amarelo (Tabebuia serratifolia), ipê-roxo (Tabebuia impetiginosa), aroeira-pimenteira (Schinus terebinthifolius) e aroeira-salsa (Schinus molle), coletadas em diferentes localidades (Piracicaba-SP, Mogi-Guaçu-SP, Rio das Pedras-SP, Ijaci-MG, Itumirim-MG e Lavras-MG) uma parte das amostras foram submetidas à assepsia e a outra não; verificar o efeito desses fungos na germinação dessas sementes; avaliar a transmissão de fungos das sementes para as plântulas; avaliar a patogenicidade de Pestalotiopsis sp. em mudas de aroeira-pimenteira e comparar o efeito de diferentes fungicidas (captam, carbendazim+tiram e carboxim+tiram) na incidência dos fungos e na germinação. Constatou-se que os fungos mais frequentes e em maior incidência encontrados associados às sementes, tanto de ipê-amarelo quanto de ipê-roxo, foram Cladosporium sp., Alternaria alternata, Epicoccum sp., Phoma sp., Geotrichum sp., Penicillium sp., Trichothecium sp., Phomopsis sp., Drechslera sp., Aspergillus spp., Curvularia sp. e Fusarium spp. De maneira geral, a assepsia proporcionou uma redução drástica de todos os fungos, em ambas espécies. Não houve diferença significativa na porcentagem de plântulas normais, entre as amostras, porém o tratamento com hipoclorito de sódio, reduziu a germinação em 64%. Na transmissão observou-se, em média, 17% e 10% de plântulas com sintomas, nas amostras sem assepsia e com assepsia, respectivamente. Os fungos mais freqüentes transmitidos pelas sementes de ipê foram: Alternaria alternata., Fusarium spp., Aspergillus spp., Phoma sp. e Phomopsis sp. Em relação às espécies de aroeira, os fungos quantificados foram Cladosporium sp., Alternaria alternata, Aspergillus spp., Pestalotiopsis sp., Penicillium sp., Fusarium spp., Epicoccum sp., Nigrospora sp., Curvularia sp., Drechslera sp., Trichoderma sp., Myrothecium sp. e Phoma sp. A assepsia reduziu ou manteve a incidência dos fungos, exceto para Pestalotiopsis sp. e Aspergillus spp. que aumentaram em algumas amostras de sementes de aroeira-pimenteira. Não houve diferença estatística da germinação das com e sem assepsia e entre amostras; para aroeira-salsa não verificou-se germinação. Foi confirmada a transmissão, principalmente dos fungos Cladosporium sp., Aspergillus spp. e Pestalotiopsis sp. Em mudas de aroeira-pimenteira verificou-se a patogenicidade de Pestalotiopsis sp. No tratamento de sementes com fungicidas, todos, de uma maneira geral, mostram resultados satisfatórios no controle de todos os fungos detectados, tanto para ipê quanto para aroeira. Captam apresentou resultado satisfatório principalmente em sementes de ipê, onde não foi verificado efeito fitotóxico em relação aos demais fungicidas comparados. Porém, o uso de copolímero de poliéter e silicone, um espalhante adesivo utilizado para facilitar a distribuição do produto, interferiu de forma negativa nos resultados de germinação de sementes de ipê; para aroeira não foi verificado este efeito. Torna-se… Advisors/Committee Members: Menten, Jose Otavio Machado.

Subjects/Keywords: Aroeira; Aroeira; Fungicidas; Fungicide; Fungos fitopatogênicos; Germinação de sementes; Ipê; Ipê; Pathogenicity; Pathogenicity fungi; Patogenicidade; Seeds germination

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Botelho, L. d. S. (2007). Fungos associados às sementes de ipê-amarelo (Tabebuia serratifolia), ipê-roxo (Tabebuia impetiginosa), aroeira-pimenteira (Schinus terebinthifolius) e aroeira-salsa (Schinus molle): incidência, efeitos na germinação, transmissão para plântulas e controle. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/11/11138/tde-08032007-142028/ ;

Chicago Manual of Style (16^th Edition):

Botelho, Luana da Silva. “Fungos associados às sementes de ipê-amarelo (Tabebuia serratifolia), ipê-roxo (Tabebuia impetiginosa), aroeira-pimenteira (Schinus terebinthifolius) e aroeira-salsa (Schinus molle): incidência, efeitos na germinação, transmissão para plântulas e controle.” 2007. Masters Thesis, University of São Paulo. Accessed March 03, 2021. http://www.teses.usp.br/teses/disponiveis/11/11138/tde-08032007-142028/ ;.

MLA Handbook (7^th Edition):

Botelho, Luana da Silva. “Fungos associados às sementes de ipê-amarelo (Tabebuia serratifolia), ipê-roxo (Tabebuia impetiginosa), aroeira-pimenteira (Schinus terebinthifolius) e aroeira-salsa (Schinus molle): incidência, efeitos na germinação, transmissão para plântulas e controle.” 2007. Web. 03 Mar 2021.

Vancouver:

Botelho LdS. Fungos associados às sementes de ipê-amarelo (Tabebuia serratifolia), ipê-roxo (Tabebuia impetiginosa), aroeira-pimenteira (Schinus terebinthifolius) e aroeira-salsa (Schinus molle): incidência, efeitos na germinação, transmissão para plântulas e controle. [Internet] [Masters thesis]. University of São Paulo; 2007. [cited 2021 Mar 03]. Available from: http://www.teses.usp.br/teses/disponiveis/11/11138/tde-08032007-142028/ ;.

Council of Science Editors:

Botelho LdS. Fungos associados às sementes de ipê-amarelo (Tabebuia serratifolia), ipê-roxo (Tabebuia impetiginosa), aroeira-pimenteira (Schinus terebinthifolius) e aroeira-salsa (Schinus molle): incidência, efeitos na germinação, transmissão para plântulas e controle. [Masters Thesis]. University of São Paulo; 2007. Available from: http://www.teses.usp.br/teses/disponiveis/11/11138/tde-08032007-142028/ ;

University of Georgia

24. Rimet, Claire-Sophie Claude Yvette. Salmonella harborage sites in infected poultry and influence of coccidiosis on the course of salmonella infection.

Degree: 2018, University of Georgia

URL: http://hdl.handle.net/10724/38525

► Salmonella is one of the leading causes of foodborne illness worldwide. The first objective of this research was to identify Salmonella harborage sites in tissues… (more)

Subjects/Keywords: Salmonella Heidelberg; Salmonella Typhimurium; Skin; Feather follicle; Ground poultry; Eimeria; Tetrathionate respiration; Salmonella Pathogenicity Island-1; Salmonella Pathogenicity Island-2

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APA (6^th Edition):

Rimet, C. C. Y. (2018). Salmonella harborage sites in infected poultry and influence of coccidiosis on the course of salmonella infection. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/38525

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rimet, Claire-Sophie Claude Yvette. “Salmonella harborage sites in infected poultry and influence of coccidiosis on the course of salmonella infection.” 2018. Thesis, University of Georgia. Accessed March 03, 2021. http://hdl.handle.net/10724/38525.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rimet, Claire-Sophie Claude Yvette. “Salmonella harborage sites in infected poultry and influence of coccidiosis on the course of salmonella infection.” 2018. Web. 03 Mar 2021.

Vancouver:

Rimet CCY. Salmonella harborage sites in infected poultry and influence of coccidiosis on the course of salmonella infection. [Internet] [Thesis]. University of Georgia; 2018. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10724/38525.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rimet CCY. Salmonella harborage sites in infected poultry and influence of coccidiosis on the course of salmonella infection. [Thesis]. University of Georgia; 2018. Available from: http://hdl.handle.net/10724/38525

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Helsinki

25. Sorsa, Liisa. The structural and functional characterization of Yersinia High-Pathogenicity islands in Escherichia coli strain Nissle 1917.

Degree: 1999, University of Helsinki

URL: http://hdl.handle.net/10138/157089

Subjects/Keywords: Pathogenicity islands; Yersinia High-Pathogenicity islands; Mutaflor; Yleinen mikrobiologia; Pathogenicity islands; Yersinia High-Pathogenicity islands; Mutaflor

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sorsa, L. (1999). The structural and functional characterization of Yersinia High-Pathogenicity islands in Escherichia coli strain Nissle 1917. (Thesis). University of Helsinki. Retrieved from http://hdl.handle.net/10138/157089

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sorsa, Liisa. “The structural and functional characterization of Yersinia High-Pathogenicity islands in Escherichia coli strain Nissle 1917.” 1999. Thesis, University of Helsinki. Accessed March 03, 2021. http://hdl.handle.net/10138/157089.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sorsa, Liisa. “The structural and functional characterization of Yersinia High-Pathogenicity islands in Escherichia coli strain Nissle 1917.” 1999. Web. 03 Mar 2021.

Vancouver:

Sorsa L. The structural and functional characterization of Yersinia High-Pathogenicity islands in Escherichia coli strain Nissle 1917. [Internet] [Thesis]. University of Helsinki; 1999. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10138/157089.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sorsa L. The structural and functional characterization of Yersinia High-Pathogenicity islands in Escherichia coli strain Nissle 1917. [Thesis]. University of Helsinki; 1999. Available from: http://hdl.handle.net/10138/157089

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

26. Galvao Ferrarini, Mariana. Metabolic Investigation of the Mycoplasmas from the Swine Respiratory Tract : Investigation métabolique des mycoplasmes dans le tractus respiratoire des cochons.

Degree: Docteur es, Bioinformatique, 2015, Université Claude Bernard – Lyon I

URL: http://www.theses.fr/2015LYO10302

►

L'appareil respiratoire des porcs est colonisé par plusieurs bactéries, parmi lesquelles trois espèces de mycoplasmes : Mycoplasma flocculare, Mycoplasma hyopneumoniae et Mycoplasma hyorhinis. Lors de… (more)

▼

L'appareil respiratoire des porcs est colonisé par plusieurs bactéries, parmi lesquelles trois espèces de mycoplasmes : Mycoplasma flocculare, Mycoplasma hyopneumoniae et Mycoplasma hyorhinis. Lors de ce doctorat, notre principal objectif était de mieux comprendre le métabolisme différentiel dans chacune des espèces à l'aide de différentes approches. Nous avons reconstruit les réseaux métaboliques complets pour toutes les souches séquencées de ces trois espèces de mycoplasmes afin d'y détecter des caractéristiques distinctes. Nous avons pu montrer que, bien que les trois espèces de mycoplasmes du porc ont des capacités métaboliques semblables, certaines différences existent qui incluent, d'une part, le catabolisme de myo-inositol et un système plus complet pour l'absorption du glycérol, et d'autre part, une large gamme de moyens d'absorption de carbohydrates chez M. hyorhinis. L'utilisation de glycérol comme source de carbone, une activité qui est absente uniquement dans M. flocculare, produit du peroxyde d'hydrogène qui est toxique, ce qui peut expliquer l'absence de pathogénicité de cette espèce. L'absorption d'un plus large éventail de sources de carbone chez M. hyorhinis peut également expliquer pourquoi cette espèce est un contaminant largement connu des cultures cellulaires. Des expériences de croissance ont montré que les milieux définis décrits pour d'autres espèces de mycoplasmes ne sont pas appropriés pour la croissance de mycoplasmes du tractues respiratoire de porcs, et que la peptone est essentielle pour le maintien de la viabilité des cellules à la fois de M. hyopneumoniae et de M. flocculare dans des milieux définis. Dans ce travail, nous proposons également de nouveaux média définis qui, in silico, sont extrêmement appropriés pour les mycoplasmes du porc. Les données de métabolomique suggèrent que même si ces espèces sont extrêmement similaires du point de vue de leurs génomes et des métabolismes, les produits et les taux de réaction diffèrent et la régulation des gènes peuvent interférer directement dans le métabolisme. Pour expliquer ces différences ainsi que d'autres décrits dans la littérature qui suggèrent que certains types de régulation de l'expression du gène existent en effet dans ces espèces, nous avons également essayé de recueillir des informations sur de nouvelles séquences promotrices. Ainsi, cette thèse servira de base pour l'étude du métabolisme différentiel et des pathologies causées par les mycoplasmes du tractus respiratoire du porc et pourra aider à proposer des façons de prévenir à l'avenir le développement des maladies associées

In this PhD thesis, we presented three main types of analyses of metabolism, and in most cases involving symbiosis: metabolic dialogue between a trypanosomatid and its symbiont, comparative analyses of metabolic networks and exploration of metabolomics data. The respiratory tract of swines is colonized by several pathogenic bacteria, among which are three mycoplasma species: Mycoplasma flocculare, Mycoplasma hyopneumoniae, and Mycoplasma hyorhinis. In…

Advisors/Committee Members: Sagot, Marie-France (thesis director), Zaha, Arnaldo (thesis director).

Subjects/Keywords: Réseaux métaboliques; Pathogenicité; Mycoplasmes; Mollicutes; Metabolic networks; Pathogenicity; Mycoplasmas; Mollicutes; 570.15

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Galvao Ferrarini, M. (2015). Metabolic Investigation of the Mycoplasmas from the Swine Respiratory Tract : Investigation métabolique des mycoplasmes dans le tractus respiratoire des cochons. (Doctoral Dissertation). Université Claude Bernard – Lyon I. Retrieved from http://www.theses.fr/2015LYO10302

Chicago Manual of Style (16^th Edition):

Galvao Ferrarini, Mariana. “Metabolic Investigation of the Mycoplasmas from the Swine Respiratory Tract : Investigation métabolique des mycoplasmes dans le tractus respiratoire des cochons.” 2015. Doctoral Dissertation, Université Claude Bernard – Lyon I. Accessed March 03, 2021. http://www.theses.fr/2015LYO10302.

MLA Handbook (7^th Edition):

Galvao Ferrarini, Mariana. “Metabolic Investigation of the Mycoplasmas from the Swine Respiratory Tract : Investigation métabolique des mycoplasmes dans le tractus respiratoire des cochons.” 2015. Web. 03 Mar 2021.

Vancouver:

Galvao Ferrarini M. Metabolic Investigation of the Mycoplasmas from the Swine Respiratory Tract : Investigation métabolique des mycoplasmes dans le tractus respiratoire des cochons. [Internet] [Doctoral dissertation]. Université Claude Bernard – Lyon I; 2015. [cited 2021 Mar 03]. Available from: http://www.theses.fr/2015LYO10302.

Council of Science Editors:

Galvao Ferrarini M. Metabolic Investigation of the Mycoplasmas from the Swine Respiratory Tract : Investigation métabolique des mycoplasmes dans le tractus respiratoire des cochons. [Doctoral Dissertation]. Université Claude Bernard – Lyon I; 2015. Available from: http://www.theses.fr/2015LYO10302

University of Georgia

27. Willingham, Jennifer Michelle. Host species tropism and the genetic requirements for intracellular replication of Rhodococcus equi.

Degree: 2017, University of Georgia

URL: http://hdl.handle.net/10724/36929

► Rhodococcus equi is an environmental Gram-positive bacterium found worldwide. In addition to being a soil-dwelling organism, it is a pathogen of young foals and pigs… (more)

▼ Rhodococcus equi is an environmental Gram-positive bacterium found worldwide. In addition to being a soil-dwelling organism, it is a pathogen of young foals and pigs who may develop severe pyogranulomatous pneumonia or abscessation of the mandibular lymph nodes, respectively. All R. equi isolates from foals contain a pVAPA-type plasmid, while the majority of swine isolates carry a pVAPB-type plasmid. pVAPA-type plasmid carriage has been demonstrated to enable intracellular replication of the bacteria in host macrophages, as well as, being critical for disease development in foals and in in vivo mouse models. The pathogenicity island (PAI) regions of pVAPA-type and pVAPB-type plasmids house a novel gene family known as the virulence associated proteins or vap family. Of interest is that vap gene composition is distinct amongst these plasmid types, with plasmids from equine isolates possessing six vap genes including the crucial, intracellular replication essential virulence determinant vapA, while plasmids from swine isolates contain vapB and six other unique vaps. The precise mechanism of action of VapA is yet to be determined, however, it has been shown to interfere with acidification of the R. equi-containing phagosome thereby promoting a niche suitable for intracellular growth. Prior to this work, the intracellular growth capabilities of pVAPB-carrying pig isolates had not been evaluated. Therefore, we first sought to analyze the intramacrophage growth potential of several swine isolates and secondly to establish whether plasmid type, pVAPA vs pVAPB, dictates host species-specific intracellular replication capabilities. Examination of various R. equi isolates containing either a pVAPA-type or pVAPB-type plasmid in macrophages of murine, equine, and swine origin revealed that while plasmid possession was critical for intracellular replication, plasmid type was not associated with species-specific intramacrophage growth. Having determined that R. equi isolates possessing pVAPB-type plasmids are capable of intramacrophage replication in the absence of vapA, the component(s) of the pVAPB-type plasmid enabling intracellular growth were investigated. Through analysis of the intramacrophage growth phenotypes of a series of deletion mutants and complementation analysis, it was determined that the pVAPB-type plasmid encoded vapK1 and vapK2 genes are required for replication in macrophages and are functionally equivalent to VapA.

Subjects/Keywords: Rhodococcus equi; vap; Species tropism; Virulence; Pathogenicity Island (PAI); pVAPA; pVAPB

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Willingham, J. M. (2017). Host species tropism and the genetic requirements for intracellular replication of Rhodococcus equi. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/36929

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Willingham, Jennifer Michelle. “Host species tropism and the genetic requirements for intracellular replication of Rhodococcus equi.” 2017. Thesis, University of Georgia. Accessed March 03, 2021. http://hdl.handle.net/10724/36929.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Willingham, Jennifer Michelle. “Host species tropism and the genetic requirements for intracellular replication of Rhodococcus equi.” 2017. Web. 03 Mar 2021.

Vancouver:

Willingham JM. Host species tropism and the genetic requirements for intracellular replication of Rhodococcus equi. [Internet] [Thesis]. University of Georgia; 2017. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/10724/36929.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Willingham JM. Host species tropism and the genetic requirements for intracellular replication of Rhodococcus equi. [Thesis]. University of Georgia; 2017. Available from: http://hdl.handle.net/10724/36929

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Federal de Mato Grosso do Sul

28. Alencar, Débora de Souza Olartechea de. Caracterização de Candida spp isoladas de hemocultivo e aspectos clínicos de candidemia .

Degree: 2013, Universidade Federal de Mato Grosso do Sul

URL: http://repositorio.cbc.ufms.br:8080/jspui/handle/123456789/1917

► O aumento de infecções invasivas por leveduras do gênero Candida representa um grave problema de saúde pública. Recentemente observa-se a emergência global de espécies de… (more)

▼ O aumento de infecções invasivas por leveduras do gênero Candida representa um grave problema de saúde pública. Recentemente observa-se a emergência global de espécies de Candida não Candida albicans como agente de candidemia. Objetivou-se neste estudo descrever as características demográficas e clínicas de 32 pacientes com diagnóstico laboratorial de candidemia, identificar as espécies envolvidas e determinar o perfil de suscetibilidade aos antifúngicos. O estudo foi realizado no Hospital Universitário de Mato Grosso do Sul em Campo Grande, Mato Grosso do Sul, Brasil, entre março de 2010 a março de 2012. A identificação fúngica foi realizada por meio de testes fenotípicos (CHROMagar Candida, microcultivo, auxanograma) e pelo sistema automatizado VITEK 2. A concentração inibitória mínima do fluconazol, voriconazol, itraconazol e anfotericina B foi determinada pelo método de microdiluição em caldo, documento M27-A3 do Clinical and Laboratory Standards Institute. A diferenciação das espécies do complexo de Candida parapsilosis foi realizada pela técnica de PCR-RFLP. Candida albicans foi a espécie mais isolada (34,4%), seguida por Candida do complexo C. parapsilosis (21,9%), C. guilliermondii (15,6%), C. glabrata (12,5%), C. tropicalis (12,5%) e C. krusei (3,1%). Candida não C. albicans foram responsáveis por 65,6% dos casos. Entre as espécies do complexo C. parapsilosis, cinco eram C. parapsilosis stricto sensu e duas C. orthopsilosis. A maioria das leveduras foi sensível aos antifúngicos testados. Todas C. glabrata foram sensíveis dose dependente ao fluconazol e resistentes ao itraconazol. Entre os cinco isolados de Candida guilliermondii, um foi sensível dose dependente ao fluconazol e todos sensíveis dose dependente ao itraconazol. A ocorrência de candidemia foi maior em pacientes com idade acima de 60 anos (46,9%) e nos grupos considerados críticos, como os internados na unidade de terapia intensiva (40,6%) e no serviço de hemodiálise (21,9%). Em três pacientes foi observada a infecção de corrente sanguínea relacionada a cateter venoso central. Dos 32 pacientes, 47% foram a óbito. A análise multivariada mostrou associação entre o maior número de óbitos em pacientes com infecção de corrente sanguínea por Candida albicans do que Candida não Candida albicans (p≤0,05). Constata-se a emergência de Candida não C. albicans como agente de candidemia no HU/UFMS, especialmente por Candida do complexo C. parapsilosis, C. guilliermondii e C. glabrata. A técnica de PCR-RFLP foi útil na diferenciação das espécies do complexo C. parapsilosis. Advisors/Committee Members: Chang, Marilene Rodrigues (advisor).

Subjects/Keywords: Candidemia - fisiopatologia; Candidemia - physiopathology; Candida - patogenicidade; Candida - pathogenicity; Fungos; Fungi

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Alencar, D. d. S. O. d. (2013). Caracterização de Candida spp isoladas de hemocultivo e aspectos clínicos de candidemia . (Thesis). Universidade Federal de Mato Grosso do Sul. Retrieved from http://repositorio.cbc.ufms.br:8080/jspui/handle/123456789/1917

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Alencar, Débora de Souza Olartechea de. “Caracterização de Candida spp isoladas de hemocultivo e aspectos clínicos de candidemia .” 2013. Thesis, Universidade Federal de Mato Grosso do Sul. Accessed March 03, 2021. http://repositorio.cbc.ufms.br:8080/jspui/handle/123456789/1917.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Alencar, Débora de Souza Olartechea de. “Caracterização de Candida spp isoladas de hemocultivo e aspectos clínicos de candidemia .” 2013. Web. 03 Mar 2021.

Vancouver:

Alencar DdSOd. Caracterização de Candida spp isoladas de hemocultivo e aspectos clínicos de candidemia . [Internet] [Thesis]. Universidade Federal de Mato Grosso do Sul; 2013. [cited 2021 Mar 03]. Available from: http://repositorio.cbc.ufms.br:8080/jspui/handle/123456789/1917.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Alencar DdSOd. Caracterização de Candida spp isoladas de hemocultivo e aspectos clínicos de candidemia . [Thesis]. Universidade Federal de Mato Grosso do Sul; 2013. Available from: http://repositorio.cbc.ufms.br:8080/jspui/handle/123456789/1917

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Penn State University

29. Kim, Hye-Seon. MOLECULAR AND CELLULAR BASIS OF FUSARIUM WILT IN ARABIDOPSIS .

Degree: 2008, Penn State University

URL: https://submit-etda.libraries.psu.edu/catalog/9289

► Although the persistence of soil-borne fungal pathogens continually poses a serious threat to the sustainability of the production of many crop plants, only a very… (more)

▼ Although the persistence of soil-borne fungal pathogens continually poses a serious threat to the sustainability of the production of many crop plants, only a very limited number of control strategies exist to manage soil-borne fungal diseases in part due to a limited understanding of the biology and ecology of soil-borne fungi. Current paradigms on the molecular and cellular basis of plant-pathogen interactions have been mostly derived from foliar diseases. The number of model systems for studying soil-borne diseases is limited. The main goal of this thesis work is to study the molecular and cellular mechanisms underpinning soil-borne fungal diseases through the use of a new model system, based on Arabidopsis thaliana as the principal host and Fusarium oxysporum as the primary pathogen. F. oxysporum causes vascular wilt and root rot diseases in a wide variety of plant species, including A. thaliana. Using a combination of molecular and cytological tools, I investigated the role of the cAMP-dependent protein kinase A gene in F. oxysporum (named as FoCPKA) in pathogenesis of Arabidopsis thaliana. Disruption of FoCPKA causes several mutant phenotypes, including complete loss of pathogenicity with no penetration/colonization of the vascular system, reduced vegetative growth and conidiation, reduced root attachment by spores, and an altered hyphal growth pattern. Preliminary functional characterization of two additional F. oxysporum genes, including NUC2 (Negative regulator of phosphorous acquisition) and ZRT2 (Iron/zinc transporter), has also been conducted. To monitor real-time cellular and physiological changes during host-pathogen interactions, I report here the first successful expression of a FRET (Fluorescence Resonance Energy Transfer)-based calcium sensor in two plant pathogenic fungi, F. oxysporum and Magnaporthe oryzae. Time-lapse imaging of live fungal cells expressing this sensor revealed the presence of transient tip high Ca2+ gradient that occurs in a pulsate manner. Examination of dynamic changes in cytosolic Ca ([Ca2+]c) suggested that Ca2+ might play important role in regulation of hyphal branching, septum formation, and cell-cell contact during fungal growth. The same sensor was also introduced into three A. thaliana ecotypes that differentially interact with F. oxysporum as a first step to investigate how host responses to fungal infection are controlled via calcium. Increased knowledge about dynamic Ca2+ changes using this Ca2+ sensor offers novel opportunities for understanding mechanisms underlying signal transduction changes in response to external stimuli during plant-pathogen interaction. A different calcium sensor and a pH sensor based on modified single green fluorescent proteins have also been developed, and their utilities for probing physiological changes in fungal and plant cells and at their interfaces are currently being evaluated. In combination with the genetic and molecular cytology tools described here, genome sequences of A. thaliana and F. oxysporum and other experimental… Advisors/Committee Members: Seogchan Kang, Committee Chair/Co-Chair, Charles Peter Romaine, Committee Member, Timothy W Mcnellis, Committee Member, Maria Del Mar Jimenez Gasco, Committee Member, Majid R Foolad, Committee Member.

Subjects/Keywords: Fusarium; Arabidopsis; Pathogenicity; Calcium biosensor

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kim, H. (2008). MOLECULAR AND CELLULAR BASIS OF FUSARIUM WILT IN ARABIDOPSIS . (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/9289

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kim, Hye-Seon. “MOLECULAR AND CELLULAR BASIS OF FUSARIUM WILT IN ARABIDOPSIS .” 2008. Thesis, Penn State University. Accessed March 03, 2021. https://submit-etda.libraries.psu.edu/catalog/9289.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kim, Hye-Seon. “MOLECULAR AND CELLULAR BASIS OF FUSARIUM WILT IN ARABIDOPSIS .” 2008. Web. 03 Mar 2021.

Vancouver:

Kim H. MOLECULAR AND CELLULAR BASIS OF FUSARIUM WILT IN ARABIDOPSIS . [Internet] [Thesis]. Penn State University; 2008. [cited 2021 Mar 03]. Available from: https://submit-etda.libraries.psu.edu/catalog/9289.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kim H. MOLECULAR AND CELLULAR BASIS OF FUSARIUM WILT IN ARABIDOPSIS . [Thesis]. Penn State University; 2008. Available from: https://submit-etda.libraries.psu.edu/catalog/9289

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas A&M University

30. Mendoza Hernández, María Regina. Argonaute 2 and Antiviral Silencing in Plants.

Degree: MS, Plant Pathology, 2016, Texas A&M University

URL: http://hdl.handle.net/1969.1/156984

► RNA silencing is a mechanism used by eukaryotes to defend them self against viruses. When encountering an external or foreign double-stranded RNA (dsRNA), plants activate… (more)

▼ RNA silencing is a mechanism used by eukaryotes to defend them self against viruses. When encountering an external or foreign double-stranded RNA (dsRNA), plants activate their machinery to assemble an RNA induced gene silencing complex (RISC) in which Argonautes (AGOs) play an important role. Once activated plant use RISC to survey for those sequences identical to the original dsRNA and cleave these into small fragments. This study provides evidence that RNA silencing against Tomato bushy stunt virus (TBSV) can depend on plant age in Nicotiana benthamiana (Nb) by using TBSV mutants that do not express P19 (TGdp19 or 157). I show that NbAGO2 plays role in older plants during viral silencing by measuring mRNA and comparing treatments. These results indicated that NbAGO2 is up-regulated after TBSV inoculation and that 6 week old plants have a better ability to silence TBSV compared to younger plants. By using transgenic plants expressing a dsRNA NbAGO2 hairpin for downregulation of NbAGO2, my studies provide evidence that NbAGO2 is required for viral silencing by conferring several layers of protection. This was demonstrated by infecting NbAGO2-hairpin transgenic plants with different TBSV variants, different Tombusvirus members and non-Tombusvirus. The results showed that reduced levels of NbAGO2 enhanced viral infection in general, these infections compromised plant integrity, TBSV not expressing the coat protein yielded severe systemic infections not otherwise observed, and TBSV not expressing P19 (TGdp19 and TBSV-157) caused more severe infections compared to controls. Additionally, my studies validate the use of two well-known viruses that express suppressors to avoid the effects of silencing by NbAGO2 or other components. This was shown for Tobacco mosaic virus (TMV) and TBSV that were used as viral vectors to express and co-express green fluorescent protein (GFP) and red fluorescent protein (RFP) in N. benthamiana and tomato. The results demonstrated that the vectors accumulate in the same plant and leaves, and even in the same cells, providing a tool for fast expression of potentially biomedically or otherwise valuable oligomeric proteins. Advisors/Committee Members: Scholthof, Herman (advisor), Scholthof, Karen-Beth G. (committee member), Qin, Hongmin (committee member).

Subjects/Keywords: AGO; N. benthamiana; plant virus; reduction pathogenicity; TBSV; TMV

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mendoza Hernández, M. R. (2016). Argonaute 2 and Antiviral Silencing in Plants. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/156984

Chicago Manual of Style (16^th Edition):

Mendoza Hernández, María Regina. “Argonaute 2 and Antiviral Silencing in Plants.” 2016. Masters Thesis, Texas A&M University. Accessed March 03, 2021. http://hdl.handle.net/1969.1/156984.

MLA Handbook (7^th Edition):

Mendoza Hernández, María Regina. “Argonaute 2 and Antiviral Silencing in Plants.” 2016. Web. 03 Mar 2021.

Vancouver:

Mendoza Hernández MR. Argonaute 2 and Antiviral Silencing in Plants. [Internet] [Masters thesis]. Texas A&M University; 2016. [cited 2021 Mar 03]. Available from: http://hdl.handle.net/1969.1/156984.

Council of Science Editors:

Mendoza Hernández MR. Argonaute 2 and Antiviral Silencing in Plants. [Masters Thesis]. Texas A&M University; 2016. Available from: http://hdl.handle.net/1969.1/156984

Open Access Theses and Dissertations