subject:( C HCV )

1. GOMES, Daniel Christiano de Albuquerque. Disfunção cognitiva em pacientes com infecção crônica pelo vírus da hepatite C: estudo transversal na cidade do Recife e revisão de literatura .

Degree: 2011, Universidade Federal de Pernambuco

URL: https://repositorio.ufpe.br/handle/123456789/29281

► A hepatite C crônica constitui atualmente importante problema de saúde pública, visto que acomete cerca de 2% da população mundial. Representa uma das principais causas… (more)

▼ A hepatite C crônica constitui atualmente importante problema de saúde pública, visto que acomete cerca de 2% da população mundial. Representa uma das principais causas de cirrose hepática, hepatocarcinoma e transplante hepático. Embora suas principais conseqüências se devam ao comprometimento hepático, manifestações extra-hepáticas da doença têm sido bem documentadas. Ao longo dos últimos anos, pesquisas com a utilização de diferentes ferramentas diagnósticas têm apontado a ocorrência de disfunção cognitiva nesta população. O presente trabalho é composto por dois artigos. O primeiro deles é um artigo de revisão, o qual visa a abordar as evidências mais atuais dos efeitos neurocognitivos da infecção crônica pelo HCV. São revisados os tipos e a prevalência de alterações cerebrais identificadas nos variados métodos diagnósticos empregados, assim como os mecanismos patogênicos propostos para a disfunção cognitiva. Embora sejam notadas controvérsias na literatura revisada, identifica-se que prejuízos em atenção, velocidade de processamento mental e memória de trabalho têm sido os mais comumente descritos na hepatite C crônica, configurando um padrão compatível com disfunção frontal-subcortical. Além disso, na parte final do artigo, são comentados os principais impactos da co-infecção HCV/HIV e da terapia antiviral à cognição.Já o segundo é um artigo original, que descreve um estudo transversal realizado em Recife, no Nordeste do Brasil. A pesquisa objetivou avaliar, através da aplicação de testes neuropsicológicos, a ocorrência de déficits cognitivos numa amostra constituída por pacientes não cirróticos com hepatite C crônica, inclusos no grupo de casos, e indivíduos curados da infecção pelo HCV, estes pertencentes ao grupo controle. Foram excluídos do estudo indivíduos com condições clínicas que sabidamente acarretam declínio cognitivo. Identificou-se elevada ocorrência de desempenho deficitário em ambos os grupos em vários dos testes aplicados, sobretudo em medidas de atenção, velocidade de processamento mental, flexibilidade mental e memória de trabalho. Diferentemente do que tem sido descrito na literatura, o presente estudo evidenciou ainda ocorrência significativa de déficits em habilidades aritméticas e construcionais visuoespaciais. Entre os grupos, houve diferenças significativas apenas em dois subtestes de linguagem, com pior desempenho do grupo de casos. Este trabalho, portanto, corroborou alguns dos achados previamente referidos na literatura, bem como evidenciou peculiaridades até então pouco descritas. Advisors/Committee Members: LOPES NETO, Edmundo Pessoa de Almeida (advisor), http://lattes.cnpq.br/1738354802121443 (advisor).

Subjects/Keywords: Hepatite C; HCV; Disfunção cognitiva

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

GOMES, D. C. d. A. (2011). Disfunção cognitiva em pacientes com infecção crônica pelo vírus da hepatite C: estudo transversal na cidade do Recife e revisão de literatura . (Masters Thesis). Universidade Federal de Pernambuco. Retrieved from https://repositorio.ufpe.br/handle/123456789/29281

Chicago Manual of Style (16^th Edition):

GOMES, Daniel Christiano de Albuquerque. “Disfunção cognitiva em pacientes com infecção crônica pelo vírus da hepatite C: estudo transversal na cidade do Recife e revisão de literatura .” 2011. Masters Thesis, Universidade Federal de Pernambuco. Accessed January 24, 2021. https://repositorio.ufpe.br/handle/123456789/29281.

MLA Handbook (7^th Edition):

GOMES, Daniel Christiano de Albuquerque. “Disfunção cognitiva em pacientes com infecção crônica pelo vírus da hepatite C: estudo transversal na cidade do Recife e revisão de literatura .” 2011. Web. 24 Jan 2021.

Vancouver:

GOMES DCdA. Disfunção cognitiva em pacientes com infecção crônica pelo vírus da hepatite C: estudo transversal na cidade do Recife e revisão de literatura . [Internet] [Masters thesis]. Universidade Federal de Pernambuco; 2011. [cited 2021 Jan 24]. Available from: https://repositorio.ufpe.br/handle/123456789/29281.

Council of Science Editors:

GOMES DCdA. Disfunção cognitiva em pacientes com infecção crônica pelo vírus da hepatite C: estudo transversal na cidade do Recife e revisão de literatura . [Masters Thesis]. Universidade Federal de Pernambuco; 2011. Available from: https://repositorio.ufpe.br/handle/123456789/29281

2. Vounatsou, Myrsini. Επιδημιολογία της λοίμωξης από τον ιό της ηπατίτιδας C σε ασθενείς κλινικής σεξουαλικών μεταδιδόμενων νοσημάτων και AIDS.

Degree: 2014, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/41110

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In the present doctoral thesis we try to record epidemiology of Hepatitis C virus (HCV) in Greece, since there are no sufficient relative scientific studies… (more)

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In the present doctoral thesis we try to record epidemiology of Hepatitis C virus (HCV) in Greece, since there are no sufficient relative scientific studies concerning our country.The collection of samples was performed in individuals who visited the Department of Sexually Transmitted Diseases and AIDS in “ANDREAS SYNGROS” Hospital during the period from 2004 till the end of 2012. 1.543 individuals were identified as positive to Hepatitis C (HCV) and these data consist the database of the present study.We applied Pearson statistical tool on this database and we attempted to statistically correlate with selective agents-variables, such as other STDs (Sexually Transmitted Diseases), age, sex, educational attainment, origin, sexual orientation, number of partners, usage of condom, usage of toxic substances, as well as with the parameter ‘economical refugee’.The laboratory methods used for the detection of HCV were the routine methods used in the Sexually Transmitted Diseases and AIDS Laboratory of ‘Andreas Syngros’ Hospital, which are immuno-serological and molecular methods. In the first case ELISA and immunoblotting methods were used, while the samples that gave uncertain result with the above mentioned methods were further proceeded with molecular detection.Studying the findings we noted that the most frequently observed groups by category are the following: in terms of toxic substances usage are the users of i.v. drugs, in terms of educational attainment are the elementary school graduates and higher education graduates, in terms of origin Greek people are far more prevalent, in terms of age the most popular group belongs to age category from 31 to 40 years old, in terms of gender men prevail, in terms of sexual orientation are the heterosexuals, in terms of number of partners are the ones with less than 5 partners during the last six months, in terms of coinfections presence the most common case is this of HIV, in terms of condom usage the highest percentage declares rare usage of condom and finally, in terms of the parameter ‘economical refugee’, only a small percentage belongs in this category.With respect to the joined variables HCV+STD, assessing a demographic characterization, our main conclusion was that only the parameter of age is representing a risk factor, while assessing an epidemiological characterization, our main conclusion was that the usage of toxic substances, the number of partners during the last six months and sexual orientation definitely are risk factors.The result of the present study is the accomplishment of an epidemiological study which can be used as a tool for clinicians, enabling them to categorize the potential carriers of the disease and contribute to early prognosis and timely treatment.

Στην παρούσα διδακτορική διατριβή γίνεται προσπάθεια καταγραφής της επιδημιολογίας του ιού της ηπατίτιδας C (HCV) στην Ελλάδα, καθώς δεν υπάρχουν αντίστοιχες επαρκείς επιστημονικές μελέτες στη χώρα μας.Η συλλογή των δειγμάτων αφορά σε άτομα που προσήλθαν στo Τμήμα Σεξουαλικώς Μεταδιδομένων…

Subjects/Keywords: Ηπατίτιδα C; Επιδημιολογία HCV; HCV-συλλοιμώξεις; ΣΜΝ; Hepatitis C; Eoidemiology HCV; HCV-HIV; HCV-coinfections; STDS

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vounatsou, M. (2014). Επιδημιολογία της λοίμωξης από τον ιό της ηπατίτιδας C σε ασθενείς κλινικής σεξουαλικών μεταδιδόμενων νοσημάτων και AIDS. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/41110

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Vounatsou, Myrsini. “Επιδημιολογία της λοίμωξης από τον ιό της ηπατίτιδας C σε ασθενείς κλινικής σεξουαλικών μεταδιδόμενων νοσημάτων και AIDS.” 2014. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed January 24, 2021. http://hdl.handle.net/10442/hedi/41110.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vounatsou, Myrsini. “Επιδημιολογία της λοίμωξης από τον ιό της ηπατίτιδας C σε ασθενείς κλινικής σεξουαλικών μεταδιδόμενων νοσημάτων και AIDS.” 2014. Web. 24 Jan 2021.

Vancouver:

Vounatsou M. Επιδημιολογία της λοίμωξης από τον ιό της ηπατίτιδας C σε ασθενείς κλινικής σεξουαλικών μεταδιδόμενων νοσημάτων και AIDS. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2014. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/10442/hedi/41110.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Vounatsou M. Επιδημιολογία της λοίμωξης από τον ιό της ηπατίτιδας C σε ασθενείς κλινικής σεξουαλικών μεταδιδόμενων νοσημάτων και AIDS. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2014. Available from: http://hdl.handle.net/10442/hedi/41110

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

3. Karamichali, Eirini. Ρύθμιση της έκφρασης του IRES (εσωτερική θέση πρόσβασης του ριβοσώματος) του ιού της ηπατίτιδας C.

Degree: 2014, University of Patras; Πανεπιστήμιο Πατρών

URL: http://hdl.handle.net/10442/hedi/35595

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HCV infection is a major public health problem and a leading cause of chronic liver disease and hepatocellular carcinoma, with approximately 180 million infected individuals… (more)

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HCV infection is a major public health problem and a leading cause of chronic liver disease and hepatocellular carcinoma, with approximately 180 million infected individuals worldwide. HCV is a positive sense RNA virus that belongs to the genus hepacivirus of the Flaviviridae family. Six major HCV genotypes (1–6) are known, each of which can be further subdivided into several subtypes (1a, 1b, 2a, etc). The HCV genome consists of a large open reading frame (ORF), flanked by highly structured 5’ and 3’ untranslated regions (UTRs). Both UTRs are conserved and control viral translation and replication. The HCV 5'-UTR contains an IRES that initiates cap-independent translation of the viral RNA. IRES-mediated translation of the HCV ORF yields a single polyprotein precursor that is co- and post-translationally processed by cellular and viral proteases, giving rise to mature structural and non structural proteins. Several studies have suggested that different cellular non canonical proteins or viral proteins can regulate the HCV IRES activity. The aim of this study was to understand the regulation of the HCV IRES dependent translation. Firstly, we tried to delineate the role of the viral proteins on HCV IRES dependent translation that still remains controversial. Clearly our studies demonstrated that HCV NS5A down-regulates IRES activity in a cell-type dependent manner. Additionally, we provide strong evidence that activated PKR up-regulates the IRES activity while silencing of endogenous PKR had the opposite effect. In addition, we concluded that the NS5A-mediated inhibitory effect on IRES-dependent translation was linked with the PKR inactivation. Moreover, as it is already reported that localised hypoxic areas are continuously present in HCC due to its high proliferation rate leading to an altered translation pattern, we investigated modulation of HCV IRES activity under low oxygen settings. Our results provided preliminary evidence that HCV-IRES-dependent translation is negatively regulated by low oxygen levels or under hypoxia-mimicking conditions in cell-specific manner.

H λοίμωξη που προκαλείται απο τον ιό της ηπατίτιδας C HCV είναι μείζον πρόβλημα για την δημόσια υγεία όπως επίσης και η κύρια αιτία της χρόνιας ηπατικής νόσου και ηπατοκυτταρικού καρκινώματος, με περίπου 180 εκατομμύρια μολυσμένα άτομα σε όλο τον κόσμο. Ο HCV είναι ένας RNA ιός θετικής πολικότητας που ανήκει στο γένος Hepacivirus της οικογένειας των Flaviviridae. Έξι γονότυποι ( 1-6 ) είναι γνωστοί, καθένας από τους οποίους μπορεί να υποδιαιρεθεί περαιτέρω σε αρκετές υποτύπους. Το γονιδίωμα του HCV αποτελείται από ένα μεγάλο ανοικτό πλαίσιο ανάγνωσης (ORF), πλαισιωμένο από ιδιαίτερα δομημένες 5 'και 3' αμετάφραστες περιοχές (UTRs). Και οι δύο περιοχές UTRs είναι συντηρημένες και έχουν τον έλεγχο της ιογενούς μετάφρασης και αναπαραγωγής. Το 5' UTR του HCV περιέχει μια περιοχή IRES απο την οποία γινεται η έναρξη της cap ανεξάρτητης μετάφρασης του ιικού RNA. Η HCV IRES εξαρτώμενη μετάφραση του ORF παράγει μια ενιαία πρόδρομη…

Subjects/Keywords: Ηπατίτιδα C, Ιός (HCV); Hepatitis C

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Karamichali, E. (2014). Ρύθμιση της έκφρασης του IRES (εσωτερική θέση πρόσβασης του ριβοσώματος) του ιού της ηπατίτιδας C. (Thesis). University of Patras; Πανεπιστήμιο Πατρών. Retrieved from http://hdl.handle.net/10442/hedi/35595

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Karamichali, Eirini. “Ρύθμιση της έκφρασης του IRES (εσωτερική θέση πρόσβασης του ριβοσώματος) του ιού της ηπατίτιδας C.” 2014. Thesis, University of Patras; Πανεπιστήμιο Πατρών. Accessed January 24, 2021. http://hdl.handle.net/10442/hedi/35595.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Karamichali, Eirini. “Ρύθμιση της έκφρασης του IRES (εσωτερική θέση πρόσβασης του ριβοσώματος) του ιού της ηπατίτιδας C.” 2014. Web. 24 Jan 2021.

Vancouver:

Karamichali E. Ρύθμιση της έκφρασης του IRES (εσωτερική θέση πρόσβασης του ριβοσώματος) του ιού της ηπατίτιδας C. [Internet] [Thesis]. University of Patras; Πανεπιστήμιο Πατρών; 2014. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/10442/hedi/35595.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Karamichali E. Ρύθμιση της έκφρασης του IRES (εσωτερική θέση πρόσβασης του ριβοσώματος) του ιού της ηπατίτιδας C. [Thesis]. University of Patras; Πανεπιστήμιο Πατρών; 2014. Available from: http://hdl.handle.net/10442/hedi/35595

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

4. Papachristou, Eleni. Μελέτη της πολυμορφίας του HCV στην Ελλάδα: αναζήτηση σπανίων στελεχών και μέθοδοι προσδιορισμού τους.

Degree: 2017, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/41393

►

The discovery of the HCV virus as the etiologic factor for the non A, non B hepatitis cases, led to the development of many methods… (more)

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The discovery of the HCV virus as the etiologic factor for the non A, non B hepatitis cases, led to the development of many methods and technologies for its detection and identification. HCV is a flavivirus and its genome is a single stranded RNA molecule which encodes for a polyprotein which provides the structural and non structural proteins of the virus. The target cells for HCV are the hepatocytes as well as B and T lymphocytes, dendritic and mononuclear cells which the virus enters throygh endocytosis. Although the immune system manages to clear the virus a large number (80-85%) of patients become chronic carriers from which about 5-25% will develop chirrossis, end stage liver disease and hepatocellular carcinoma.HCV has a great genetic diversity, is spread worldwide having infected more than 150 million of people and is classified in seven major genotypes and many subtypes. The detection and quantification of thw HCV RNA are valuable tools for monitoring the chronic infection and the outcome of the therapy. Furthermore, knowing the genotype with which a patient is infected is crucial for the choice of the therary regimen. The aim of this study was to develop two in house methods a) for the detection and quantification of the HCV RNA and b) for the determination of the genotype in order to study the diversity of HCV in Greece.For the quantification method we targeted at the 3’UTR end of the genome in contrast to all the commercially available methods which target the 5’end. After experimenting with different types of reagents and enzymes and on different platforms we finally chose the technology of one step real time RT PCR on the Light Cycler platform (Roche). The method was validated with international standards and was evaluated with two commercially available methods from Abbott and Roche with excellent results. The method is quick, reliable and low cost and can be further developed.For the HCV genotyping we developed a method based on the sequencing of the NS5B region of the genome. We designed primers and tried many PCR protocols. The obtained sequences were analysed with phylogenetic analysis methods. The method was validated with international standards and was certified by ΕΣΥΔ. With this method we genotyped most of the undetermined samples (by other methods) in our lab and mainly we studied the HCV dispersal pattern among IDUs (intravenous drug users) in Athens as part of the “Aristotle” programme.

Η ανακάλυψη και ταυτοποίηση του ιού HCV ως τον αιτιολογικό παράγοντα των περιστατικών μη-Α, μη-Β ηπατίτιδας ήταν η αρχή για την ανάπτυξη διαφόρων μεθόδων και τεχνολογιών για την ανίχνευση και τυποποίησή του. Ο ιός HCV ανήκει στην οικογένεια Flaviviridae, γένος hepacivirus και το γονιδίωμά του είναι μονόκλωνο RNA θετικής πολικότητας που κωδικοποιεί μια πολυπρωτεϊνη από την οποία προκύπτουν οι δομικές και οι μη δομικές πρωτεϊνες του. Κύτταρα-στόχοι του ιού – στα οποία εισέρχεται με ενδοκύτωση μετά από πρόσδεση σε ειδικούς υποδοχείς- είναι τα ηπατοκύτταρα αλλά αναφέρονται και τα λεμφοκύτταρα (Β και Τ) καθώς…

Subjects/Keywords: Ηπατίτιδα C; HCV-RNA; Γονότυπος HCV; ΧΕΝ; Δίκτυα μετάδοσης; Hepatitis C; HCV-RNA; HCV genotype; IDU; Transmission networks

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Papachristou, E. (2017). Μελέτη της πολυμορφίας του HCV στην Ελλάδα: αναζήτηση σπανίων στελεχών και μέθοδοι προσδιορισμού τους. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/41393

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Papachristou, Eleni. “Μελέτη της πολυμορφίας του HCV στην Ελλάδα: αναζήτηση σπανίων στελεχών και μέθοδοι προσδιορισμού τους.” 2017. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed January 24, 2021. http://hdl.handle.net/10442/hedi/41393.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Papachristou, Eleni. “Μελέτη της πολυμορφίας του HCV στην Ελλάδα: αναζήτηση σπανίων στελεχών και μέθοδοι προσδιορισμού τους.” 2017. Web. 24 Jan 2021.

Vancouver:

Papachristou E. Μελέτη της πολυμορφίας του HCV στην Ελλάδα: αναζήτηση σπανίων στελεχών και μέθοδοι προσδιορισμού τους. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2017. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/10442/hedi/41393.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Papachristou E. Μελέτη της πολυμορφίας του HCV στην Ελλάδα: αναζήτηση σπανίων στελεχών και μέθοδοι προσδιορισμού τους. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2017. Available from: http://hdl.handle.net/10442/hedi/41393

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Indian Institute of Science

5. Bhat, Prasanna. Characterization of Host Protein Interactions with HCV RNA : Implications in Viral Translation, Replication and Design of Antivirals.

Degree: PhD, Faculty of Science, 2018, Indian Institute of Science

URL: http://etd.iisc.ac.in/handle/2005/3496

► HCV genome is a positive sense single-stranded RNA containing a single open reading frame (ORF) flanked by untranslated regions (UTRs), 5’UTR and 3’UTR.Initiation of HCV… (more)

▼ HCV genome is a positive sense single-stranded RNA containing a single open reading frame (ORF) flanked by untranslated regions (UTRs), 5’UTR and 3’UTR.Initiation of HCV RNA translation is mediated by internal ribosome entry site (IRES) present in 5’ UTR and this process is independent of cap-structure and requires only a small subset of canonical initiation factors. Hence, HCV IRES-mediated translation initiation mechanism is quite different from canonical cellular mRNA translation initiation. The IRES is organized into highly structured domains, namely domain II, III and IV. High affinity interactions between structured RNA elements present in the IRES and 40S ribosomal proteins mediate 40S recruitment to HCV IRES. However, details of the RNA elements and region of ribosomal proteins involved in these interactions are poorly understood. In recent days, RNA-based molecules like siRNAs, antisense RNAs and RNA decoys have become promising candidates for antiviral molecules. So designing short RNA molecules that target unique HCV translation initiation mechanism might help in developing novel anti-HCV molecules. HCV 3’UTR and antisense-5’ UTRs serve as sites for replication initiation to synthesize negative and positive strand and this process is catalyzed by NS5B protein (RNA-dependent RNA polymerase). Hence, host proteins binding to both 3’UTR and antisense-5’UTR might play important role in HCV replication. This puts the study of HCV RNA–host protein interactions and its role in viral translation and replication in perspective. Studying the HCV IRES-ribosomal protein S5 interactions and its role in HCV IRES function Previous studies from our laboratory have demonstrated that binding of La protein to GCAC close to initiator AUG enhances ribosomal protein S5 (RPS5) binding with HCV IRES and stimulates HCV translation. However in-detail study on HCV IRES–RPS5 interactions and its implication on HCV translation initiation were lacking. In present study computational modelling suggested that domain II and IV interact majorly with the beta hairpin structure and C-terminal helix of RPS5. Filter-binding and UV cross-linking studies with peptides derived from predicated RNA-binding region of RPS5 and mutational studies with RPS5 demonstrated that beta hairpin structure present in RPS5 is critical for IRES–RPS5 interaction. In parallel, we have studied RNA elements involved in the IRES–RPS5 interactions using deletions and substitution mutations, which we had generated on the basis of the computational model. Direct and competition UV cross-linking experiments performed with these IRES mutants and 40S subunits as a source of RPS5 suggested that structure and sequence of both domain II and IV play crucial role in IRES–RPS5 interactions. We further investigated the effect of these mutations on IRES activity by in vitro translation assay and found that all the mutants that were compromised in binding to RPS5 showed reduced IRES activity. Moreover, ribosome assembly experiments on HCV IRES demonstrated that mutations affecting… Advisors/Committee Members: Das, Saumitra (advisor).

Subjects/Keywords: Hepatitis C Virus (HCV) RNA; HCV RNA Host Protein Interactions; HVC Infection; HCV RNA Translation; HCV Genomes; HCV Replication; HCV IRES RNA; HCV IRES; HCV Replication; HCV-IRES; HCV-IRES; HCV RNA; Virus-Host Protein Interactions; Viral RNA Replication; Microbiology and Cell Biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bhat, P. (2018). Characterization of Host Protein Interactions with HCV RNA : Implications in Viral Translation, Replication and Design of Antivirals. (Doctoral Dissertation). Indian Institute of Science. Retrieved from http://etd.iisc.ac.in/handle/2005/3496

Chicago Manual of Style (16^th Edition):

Bhat, Prasanna. “Characterization of Host Protein Interactions with HCV RNA : Implications in Viral Translation, Replication and Design of Antivirals.” 2018. Doctoral Dissertation, Indian Institute of Science. Accessed January 24, 2021. http://etd.iisc.ac.in/handle/2005/3496.

MLA Handbook (7^th Edition):

Bhat, Prasanna. “Characterization of Host Protein Interactions with HCV RNA : Implications in Viral Translation, Replication and Design of Antivirals.” 2018. Web. 24 Jan 2021.

Vancouver:

Bhat P. Characterization of Host Protein Interactions with HCV RNA : Implications in Viral Translation, Replication and Design of Antivirals. [Internet] [Doctoral dissertation]. Indian Institute of Science; 2018. [cited 2021 Jan 24]. Available from: http://etd.iisc.ac.in/handle/2005/3496.

Council of Science Editors:

Bhat P. Characterization of Host Protein Interactions with HCV RNA : Implications in Viral Translation, Replication and Design of Antivirals. [Doctoral Dissertation]. Indian Institute of Science; 2018. Available from: http://etd.iisc.ac.in/handle/2005/3496

Indian Institute of Science

6. Kumar, Anuj. Mechanistic Insights into Translation and Replication of Hepatitis C Virus RNA : Exploring Direct-Acting Antivirals.

Degree: PhD, Faculty of Science, 2018, Indian Institute of Science

URL: http://etd.iisc.ac.in/handle/2005/3260

► Hepatitis C virus (HCV), a blood-borne pathogen, is a small enveloped RNA virus belonging to the Hepacivirus genus of the Flaviviridae family. HCV infection represents… (more)

▼ Hepatitis C virus (HCV), a blood-borne pathogen, is a small enveloped RNA virus belonging to the Hepacivirus genus of the Flaviviridae family. HCV infection represents one of the major health concerns affecting approximately 170 million people globally. Patients with chronic HCV infection are at risk of developing hepatic fibrosis, cirrhosis and hepatocellular carcinoma. No protective anti-HCV vaccine is available yet. Until recently, standard therapy based on pegylated interferon plus ribavirin, was inadequate in treating all the patients as it results in a sustained virological response in only 40 to 50 percent of patients infected with the most common genotype (gt 1). Advances in understanding host-HCV interactions have helped developing newer anti-HCV agents such as telaprevir and boceprevir. However, treatment success is still limited due to different factors including genotype specificity, high cost, potential drug-drug interactions, substantial side effects etc. The positive-sense single-stranded RNA genome of HCV is approximately 9.6kb long which is flanked by highly structured and conserved 5’ and 3’ untranslated regions (UTRs) at both ends. Unlike cap-dependent translation of host cell mRNAs, HCV translation is mediated by an internal ribosomal entry site (IRES) present majorly within the 5’UTR. Several reports have demonstrated the interaction of different cellular proteins with HCV-5’UTR and/or 3’UTR, which include human La protein, polypyrimidine tract binding protein (PTB), poly (rC)-binding protein 2 (PCBP2) etc. These interactions of trans-acting factors with the UTRs may be important for HCV translation and/or replication. Earlier study from our laboratory revealed the importance of interaction of human La protein, by its central RNA recognition motif (RRM), with the HCV IRES around a tetranucleotide sequence GCAC near initiator AUG in influencing HCV translation. However, the role of this interaction, if any, in HCV RNA replication was not known. In the first part of the thesis, we characterized the interaction between human La protein and the GCAC to understand its role in HCV replication. We incorporated mutation, which altered the binding of La, in the GCAC motif in HCV monocistronic replicon and checked HCV RNA replication by reverse transcriptase polymerase chain reaction (RT-PCR). The mutation drastically inhibited HCV replication. Interestingly, overexpression of La could reverse the effect of this mutation and significantly enhanced HCV RNA levels. Using a bicistronic replicon, we observed that decrease in replication was independent of translation inhibition. Furthermore, mutation at the GCAC motif reduced the association between La and viral polymerase, NS5B as seen in co-immunoprecipitation assays. Moreover, this mutation affected translation to replication switch regulated by the interplay between HCV-NS3 protease and human La protein. Our analyses of point mutations, based on RT-PCR and luciferase assays, revealed distinct roles of each nucleotide of the GCAC motif in HCV replication… Advisors/Committee Members: Das, Saumitra (advisor).

Subjects/Keywords: Hepatitis C Virus RNA; HCV Genotypes; HCV Vaccines; Hepatitis C Virus Infection; Antivirals; Human La Protein; Hepatitis C Virus Life Cycle; Viral Proteins; HCV Replication; HCV IRES; HCV Translation; HCV Infection; HCV Vaccine Development; Hepatitis C Virus (HCV); Microbiology and Cell Biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kumar, A. (2018). Mechanistic Insights into Translation and Replication of Hepatitis C Virus RNA : Exploring Direct-Acting Antivirals. (Doctoral Dissertation). Indian Institute of Science. Retrieved from http://etd.iisc.ac.in/handle/2005/3260

Chicago Manual of Style (16^th Edition):

Kumar, Anuj. “Mechanistic Insights into Translation and Replication of Hepatitis C Virus RNA : Exploring Direct-Acting Antivirals.” 2018. Doctoral Dissertation, Indian Institute of Science. Accessed January 24, 2021. http://etd.iisc.ac.in/handle/2005/3260.

MLA Handbook (7^th Edition):

Kumar, Anuj. “Mechanistic Insights into Translation and Replication of Hepatitis C Virus RNA : Exploring Direct-Acting Antivirals.” 2018. Web. 24 Jan 2021.

Vancouver:

Kumar A. Mechanistic Insights into Translation and Replication of Hepatitis C Virus RNA : Exploring Direct-Acting Antivirals. [Internet] [Doctoral dissertation]. Indian Institute of Science; 2018. [cited 2021 Jan 24]. Available from: http://etd.iisc.ac.in/handle/2005/3260.

Council of Science Editors:

Kumar A. Mechanistic Insights into Translation and Replication of Hepatitis C Virus RNA : Exploring Direct-Acting Antivirals. [Doctoral Dissertation]. Indian Institute of Science; 2018. Available from: http://etd.iisc.ac.in/handle/2005/3260

University of Adelaide

7. Tse, Edmund. Improving treatment predictors of HCV therapy and the impact of steatosis on the hepatocyte transcriptome and anti-HCV action of interferon.

Degree: 2015, University of Adelaide

URL: http://hdl.handle.net/2440/100718

► Hepatitis C Virus (HCV) is a significant global health issue that leads to the development of chronic liver inflammation, and subsequent establishment of cirrhosis and… (more)

▼ Hepatitis C Virus (HCV) is a significant global health issue that leads to the development of chronic liver inflammation, and subsequent establishment of cirrhosis and hepatocellular carcinoma (HCC). Previously the standard therapy of chronic hepatitis C (CHC) was pegylated interferon α (IFN-α) and ribavirin, which had poor treatment success rates and was associated with significant side effects. Risk factors that have been shown to be associated with treatment failure include excess alcohol consumption, advanced age, diabetes and obesity. Although these negative predictors of treatment outcome have been well established in clinical practice, little is known regarding the molecular mechanism(s) of treatment failure. Obesity is another major health issue which is associated with numerous deleterious health issues, one of which being steatosis, or non-alcoholic fatty liver disease (NAFLD). NAFLD can progress to necroinflammation of the liver or non-alcoholic steatohepatitis (NASH), leading to fibrosis and development of cirrhosis. Given how common obesity is, clinicians are commonly faced with managing patients with CHC and concurrent steatosis. Understanding the molecular mechanism(s) of interferonbased treatment failure in patients with CHC with concurrent steatosis, may allow adjuvant therapy to be targeted to those with negative predictors of treatment outcome, thus resulting in an improved virological response. In this thesis, an in vitro model of steatosis has been adopted to investigate the effect of lipid loading on gene expression, in particular, interferon-stimulated genes (ISGs). Two different free fatty acids, oleic acid and palmitic acid, were used to induce steatosis in the Huh-7 hepatoma cell line. In this thesis, it was shown that steatosis was associated with a marked alteration of gene expression, some of which interestingly were classical ISGs. This was likely due to TLR2 mediated pathways, leading to subsequent downstream NF-κβ activation and gene expression. Through induction of steatosis by oleic and palmitic acid, it was also shown that Huh- 7 cells can accentuate the effect of interferon stimulation, leading to an increased ISG expression, which is believed to be secondary to the increase in STAT1 phosphorylation. Finally the effect of steatosis-induced ISG expression on HCV replication, as well as the responsiveness to IFN-α treatment, was investigated. Surprisingly, it was found that steatosis alone led to a modest reduction of HCV replication, with reduced interferon sensitivity, leading to a reduction in HCV knockdown when IFN-α was used. It was shown that the combination of OA:PA, HCV replication and IFN-α stimulation resulted in a significant increase in CXCL8 protein production, a cytokine known to have anti-IFN modulating activity. Moreover, exogenous addition of CXCL8 to cultured cells abrogated the anti-HCV actions of IFN-α. This highlighted a potential mechanism for IFN failure in the HCV infected liver with concurrent steatosis. In summary, the in vitro model of steatosis has… Advisors/Committee Members: Beard, Michael Robert (advisor), School of Biological Sciences (school).

Subjects/Keywords: HCV; hepatitis C; steatosis; interferon; hepatocyte transcriptome

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tse, E. (2015). Improving treatment predictors of HCV therapy and the impact of steatosis on the hepatocyte transcriptome and anti-HCV action of interferon. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/100718

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tse, Edmund. “Improving treatment predictors of HCV therapy and the impact of steatosis on the hepatocyte transcriptome and anti-HCV action of interferon.” 2015. Thesis, University of Adelaide. Accessed January 24, 2021. http://hdl.handle.net/2440/100718.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tse, Edmund. “Improving treatment predictors of HCV therapy and the impact of steatosis on the hepatocyte transcriptome and anti-HCV action of interferon.” 2015. Web. 24 Jan 2021.

Vancouver:

Tse E. Improving treatment predictors of HCV therapy and the impact of steatosis on the hepatocyte transcriptome and anti-HCV action of interferon. [Internet] [Thesis]. University of Adelaide; 2015. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2440/100718.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tse E. Improving treatment predictors of HCV therapy and the impact of steatosis on the hepatocyte transcriptome and anti-HCV action of interferon. [Thesis]. University of Adelaide; 2015. Available from: http://hdl.handle.net/2440/100718

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

8. MONAGHAN, ANN MARIE. An Examination of Physical Activity, Cardiorespiratory Fitness and Cardio-metabolic Health through the Modern Hepatitis C Continuum.

Degree: School of Medicine. Discipline of Physiotherapy, 2019, Trinity College Dublin

URL: http://hdl.handle.net/2262/86491

► Hepatitis C (HCV) is an established global health issue with an approximated worldwide incidence of 3%. Hepatic features of chronic hepatitis C; such as hepatic… (more)

▼ Hepatitis C (HCV) is an established global health issue with an approximated worldwide incidence of 3%. Hepatic features of chronic hepatitis C; such as hepatic fibrosis, steatosis and cirrhosis, and extrahepatic features such as insulin resistance, type 2 diabetes and atherosclerosis can predispose individuals with HCV to an elevated risk of both liver-related and cardiovascular-related morbidity and mortality, in comparison to the general population. Obesity, physical inactivity and poor cardiorespiratory fitness further compounds this risk. The protective effects of regular moderate to vigorous-intensity physical activity and thus adequate cardiorespiratory fitness on all-cause morbidity and mortality are well established in the general population. Additionally, the anti-inflammatory attributes of exercise have the potential to ameliorate both hepatic and extra hepatic features of chronic hepatitis C. Therefore, this thesis aimed to assess physical activity, cardiorespiratory fitness and measures of cardio-metabolic health throughout the modern HCV continuum, with a view to determining the necessity and merit of an aerobic exercise intervention in this cohort. Work for this thesis commenced with a literature review investigating the existing research in the area of aerobic exercise and physical activity in the management of hepatic and extrahepatic features of hepatitis C. Following on from this was Study 1a, a cross sectional examination of habitual physical activity, cardiorespiratory fitness and cardio-metabolic health in 155 individuals living with chronic hepatitis C. Low levels of physical activity and poor cardiorespiratory fitness were a prominent feature in the cohort The results of Study 1a prompted 3 further studies; (i) an investigation of the effects of achieving sustained virological response on habitual physical activity, cardiorespiratory fitness levels and measures of cardio-metabolic health in individuals with hepatitis C (Study 1b) (ii) an examination of perceived barriers and motivators to engaging in physical activity in individuals living with hepatitis C (Study 2), and (iii), an investigation of respiratory function, cardiorespiratory fitness and physical activity in both HCV RNA positive and HCV RNA negative persons who currently engage in polysubstance use (Study 3). Collectively, the results of this thesis indicate poor levels of physical activity, cardiorespiratory fitness and widespread obesity in Irish individuals living with chronic hepatitis C. Furthermore, dyslipidaemia was apparent at SVR24, demonstrating the need for extended monitoring of cardio-metabolic health profile post DAA therapy. Moreover, this indicates a specific and prescriptive aerobic exercise regimen in this cohort warrants investigation, both in treated and untreated individuals with hepatitis C. However, challenges present in terms of study recruitment and attendance. Thus, preliminary strategies aimed at enacting positive health behaviour change and an examination of the feasibility of conducting such lifestyle… Advisors/Committee Members: Gormley, John.

Subjects/Keywords: Hepatitis C; HCV; Physical Activity; Cardiorespiratory Fitness

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

MONAGHAN, A. M. (2019). An Examination of Physical Activity, Cardiorespiratory Fitness and Cardio-metabolic Health through the Modern Hepatitis C Continuum. (Thesis). Trinity College Dublin. Retrieved from http://hdl.handle.net/2262/86491

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

MONAGHAN, ANN MARIE. “An Examination of Physical Activity, Cardiorespiratory Fitness and Cardio-metabolic Health through the Modern Hepatitis C Continuum.” 2019. Thesis, Trinity College Dublin. Accessed January 24, 2021. http://hdl.handle.net/2262/86491.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

MONAGHAN, ANN MARIE. “An Examination of Physical Activity, Cardiorespiratory Fitness and Cardio-metabolic Health through the Modern Hepatitis C Continuum.” 2019. Web. 24 Jan 2021.

Vancouver:

MONAGHAN AM. An Examination of Physical Activity, Cardiorespiratory Fitness and Cardio-metabolic Health through the Modern Hepatitis C Continuum. [Internet] [Thesis]. Trinity College Dublin; 2019. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2262/86491.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

MONAGHAN AM. An Examination of Physical Activity, Cardiorespiratory Fitness and Cardio-metabolic Health through the Modern Hepatitis C Continuum. [Thesis]. Trinity College Dublin; 2019. Available from: http://hdl.handle.net/2262/86491

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Montana

9. Snyder, Blair Rice. Examining the Impact of Hepatitis C in Montana: A Descriptive Case Study.

Degree: MS, 2013, University of Montana

URL: https://scholarworks.umt.edu/etd/407

► The purpose of this study was to gather information about how Hepatitis C (HCV) affects Montanans. Montana specific information was collected about HCV transmission; factors… (more)

▼ The purpose of this study was to gather information about how Hepatitis C (HCV) affects Montanans. Montana specific information was collected about HCV transmission; factors influencing transmission; physical, social and psychological effects of having HCV and undergoing treatment; barriers to prevention and treatment; current available resources to those infected with HCV and ways to improve prevention and treatment. Secondary data consisted of a comprehensive literature review to describe the above factors and epidemiological information. Primary data was collected through key informant interviews and summary reports completed by people living with HCV. The findings suggest that HCV is primarily transmitted through the use of contaminated needles to inject drugs in Montana; Montana Law prohibits needle exchange programs. Although the literature and key informants confirmed that poverty is an environmental factor that contributes to the spread of HCV, HCV positive participants did not concur; therefore, the relationship between poverty and the spread of HCV remains undefined. Other environmental factors that were found to significantly contribute to the spread of HCV in Montana are the lack of access to clean needles, lack of public education and awareness and the prison and jail systems. The physical, social and psychological effects of not only having HCV, but being treated for HCV, were found to be tremendous. Treatment costs, lack of knowledge, difficulty of treatment, lack of access to treatment, the slow progression of the infection, having to be clean and sober before starting treatment and the stigma and lack of knowledge among physicians were all found to be large barriers to seeking treatment. Barriers to prevention included the lack of education and funding, stigma, and having few prevention options. Increasing media, awareness, and education were highlighted as the best ways to improve prevention. In order to improve treatment, it is necessary to not only decrease the cost, but also make it more available throughout the state of Montana. The findings from this study will be used by the Montana Department of Public Health and Human Services to increase awareness of how HCV impacts Montana residents.

Subjects/Keywords: Descriptive Case Study; HCV; Hepatitis C

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Snyder, B. R. (2013). Examining the Impact of Hepatitis C in Montana: A Descriptive Case Study. (Masters Thesis). University of Montana. Retrieved from https://scholarworks.umt.edu/etd/407

Chicago Manual of Style (16^th Edition):

Snyder, Blair Rice. “Examining the Impact of Hepatitis C in Montana: A Descriptive Case Study.” 2013. Masters Thesis, University of Montana. Accessed January 24, 2021. https://scholarworks.umt.edu/etd/407.

MLA Handbook (7^th Edition):

Snyder, Blair Rice. “Examining the Impact of Hepatitis C in Montana: A Descriptive Case Study.” 2013. Web. 24 Jan 2021.

Vancouver:

Snyder BR. Examining the Impact of Hepatitis C in Montana: A Descriptive Case Study. [Internet] [Masters thesis]. University of Montana; 2013. [cited 2021 Jan 24]. Available from: https://scholarworks.umt.edu/etd/407.

Council of Science Editors:

Snyder BR. Examining the Impact of Hepatitis C in Montana: A Descriptive Case Study. [Masters Thesis]. University of Montana; 2013. Available from: https://scholarworks.umt.edu/etd/407

Freie Universität Berlin

10. Reumuth, Georg. drei Jahrzehnte monozentrischer Erfahrung.

Degree: 2020, Freie Universität Berlin

URL: http://dx.doi.org/10.17169/refubium-27875

► Die Hepatitis-C-Infektion war über einen langen Zeitraum, aufgrund der fehlenden medikamentösen Behandlungsmöglichkeiten, die führende Indikation zur Lebertransplantation. Der postoperative Verlauf war gekennzeichnet durch eine Rekurrenz… (more)

▼ Die Hepatitis-C-Infektion war über einen langen Zeitraum, aufgrund der fehlenden medikamentösen Behandlungsmöglichkeiten, die führende Indikation zur Lebertransplantation. Der postoperative Verlauf war gekennzeichnet durch eine Rekurrenz der Grunderkrankung mit entsprechender Entwicklung einer fortgeschrittenen Fibrose bzw. Zirrhose bis hin zum Transplantatversagen. In einer Ära der großen medizinischen Entwicklungen hat sich die HCV-Therapie von einer wenig effektiven, mit schweren Nebenwirkungen behafteten zu einer sicheren und nebenwirkungsarmen Behandlungsmethode entwickelt. In dieser Untersuchung werden drei Jahrzehnte der Lebertransplantation aufgrund eines Hepatitis-C-assoziierten-Leberversagens ausgewertet und die entscheidenden Entwicklungsschritte rausgearbeitet. Methodik: Es werden n = 529 Hepatitis-C-positive Patienten nach Lebertransplantation zwischen 1989 und 2017 an der Charité Berlin eingeschlossen und bezüglich demografischer Daten, Inflammation, Fibrose, HCV-Genotyp, antiviraler Therapie, Retransplantation, HCV-Rekurrenz und Überleben untersucht. Ergebnisse: Es wird eine signifikante Verbesserung des Therapieerfolges der antiviralen Therapie (p < 0.01) der interferonfreien Therapieregime gegenüber früherer Behandlungsansätze nachgewiesen. Die Progression der Fibrose verläuft unter Einsatz einer antiviralen Medikation signifikant langsamer. Die HCV-Rekurrenz nach Lebertransplantation entwickelt sich im Laufe der drei Jahrzehnte von 100 % auf 0 % pro Jahr (p < 0.01). Weiterhin kann verdeutlicht werden, dass eine fortgeschrittene, frühe Fibrose/Inflammation, die medikamentöse Therapie, das Vorliegen eines Hepatozellulären Karzinoms sowie der HCV-Genotyp 1b signifikanten Einfluss auf das Überleben haben. Der Allokationsmodus hingegen hat einen nachrangigen Einfluss. Schlussfolgerung: In dieser Untersuchung können die Entwicklung sowie die verschiedenen Epochen der medikamentösen Behandlung der HCV-assoziierten Lebertransplantation aufgearbeitet werden. Die unterschiedlichen Phasen reichen von einer nicht heilbaren Erkrankung mit der Lebertransplantation als Endglied der Behandlungskette mit mäßigen Ergebnissen bis hin zur sicher und nebenwirkungsarm zu behandelnden Infektionserkrankung mit Auswirkung auf die Notwendigkeit der Lebertransplantation und auf den postoperativen Verlauf. Advisors/Committee Members: male (gender), N.N. (firstReferee), N.N. (furtherReferee).

Subjects/Keywords: Hepatitis C; liver transplantation; HCV; ddc:610

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Reumuth, G. (2020). drei Jahrzehnte monozentrischer Erfahrung. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-27875

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Reumuth, Georg. “drei Jahrzehnte monozentrischer Erfahrung.” 2020. Thesis, Freie Universität Berlin. Accessed January 24, 2021. http://dx.doi.org/10.17169/refubium-27875.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Reumuth, Georg. “drei Jahrzehnte monozentrischer Erfahrung.” 2020. Web. 24 Jan 2021.

Vancouver:

Reumuth G. drei Jahrzehnte monozentrischer Erfahrung. [Internet] [Thesis]. Freie Universität Berlin; 2020. [cited 2021 Jan 24]. Available from: http://dx.doi.org/10.17169/refubium-27875.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Reumuth G. drei Jahrzehnte monozentrischer Erfahrung. [Thesis]. Freie Universität Berlin; 2020. Available from: http://dx.doi.org/10.17169/refubium-27875

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

11. Καραμιχάλη, Ειρήνη. Ρύθμιση της έκφρασης του IRES (εσωτερική θέση πρόσβασης του ριβοσώματος) του ιού της ηπατίτιδας C.

Degree: 2014, University of Patras

URL: http://hdl.handle.net/10889/8120

►

H λοίμωξη που προκαλείται απο τον ιό της ηπατίτιδας C HCV είναι μείζον πρόβλημα για την δημόσια υγεία όπως επίσης και η κύρια αιτία της… (more)

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H λοίμωξη που προκαλείται απο τον ιό της ηπατίτιδας C HCV είναι μείζον πρόβλημα για την δημόσια υγεία όπως επίσης και η κύρια αιτία της χρόνιας ηπατικής νόσου και ηπατοκυτταρικού καρκινώματος, με περίπου 180 εκατομμύρια μολυσμένα άτομα σε όλο τον κόσμο. Ο HCV είναι ένας RNA ιός θετικής πολικότητας που ανήκει στο γένος Hepacivirus της οικογένειας των Flaviviridae. Έξι γονότυποι ( 1-6 ) είναι γνωστοί, καθένας από τους οποίους μπορεί να υποδιαιρεθεί περαιτέρω σε αρκετές υποτύπους. Το γονιδίωμα του HCV αποτελείται από ένα μεγάλο ανοικτό πλαίσιο ανάγνωσης (ORF), πλαισιωμένο από ιδιαίτερα δομημένες 5 'και 3' αμετάφραστες περιοχές (UTRs). Και οι δύο περιοχές UTRs είναι συντηρημένες και έχουν τον έλεγχο της ιογενούς μετάφρασης και αναπαραγωγής. Το 5' UTR του HCV περιέχει μια περιοχή IRES απο την οποία γινεται η έναρξη της cap ανεξάρτητης μετάφρασης του ιικού RNA. Η HCV IRES εξαρτώμενη μετάφραση του ORF παράγει μια ενιαία πρόδρομη πολυπρωτεΐνη που μεσω μετα-μεταφραστικής τροποποίησης από κυτταρικές και ιικές πρωτεάσες, οδηγεί στην ωρίμανση δομικών και μη δομικών πρωτεϊνών. Αρκετές μελέτες έχουν δείξει ότι διαφορετικές κυτταρικές ή ιικές πρωτεΐνες μπορούν να ρυθμίσουν την ενεργότητα του HCV IRES. Στόχος της παρούσας μελέτης ήταν η κατανόηση της ρύθμισης της HCV IRES εξαρτώμενης μετάφρασης. Πρώτον, προσπαθήσαμε να ορίσουμε το ρόλο των ιικών πρωτεϊνών στην HCV IRES εξαρτώμενη μετάφραση που παραμένει αμφιλεγόμενος. Σαφώς, οι μελέτες μας έδειξαν ότι η HCV NS5A ρυθμίζει αρνητικά την ενεργότητα του IRES με κύτταρο-εξαρτώμενο τρόπο. Επιπρόσθετα, υπάρχουν ισχυρές ενδείξεις ότι η ενεργοποιημένη PKR ρυθμίζει θετικά την ενεργότητα του IRES ενώ η καταστολή της έκφρασης της ενδογενούς PKR έχει το αντίθετο αποτέλεσμα. Επιπλέον, καταλήξαμε στο συμπέρασμα ότι η NS5A μεσολαβεί ανασταλτικά στην IRES-εξαρτώμενη μετάφραση και συνδέεται με την αδρανοποίηση της PKR. Τέλος στην παρούσα εργασία ερευνήσαμε τη ρύθμιση της ενεργότητας του HCV IRES σε συνθήκες χαμηλού οξυγόνου, δεδομένου ότι υποξικές περιοχές εντοπίζονται στον ηπατοκυτταρικό καρκίνο (HCC) και συνδέονται με την ύπαρξη ενός εναλλακτικού προφίλ κυτταρικής μετάφρασης Τα αποτελέσματά μας δείχνουν ότι η HCV IRES-εξαρτώμενη μετάφραση ρυθμίζεται αρνητικά σε υποξικές συνθήκες και μάλιστα με κύτταρο-εξαρτώμενο τρόπο.

HCV infection is a major public health problem and a leading cause of chronic liver disease and hepatocellular carcinoma, with approximately 180 million infected individuals worldwide. HCV is a positive sense RNA virus that belongs to the genus hepacivirus of the Flaviviridae family. Six major HCV genotypes (1–6) are known, each of which can be further subdivided into several subtypes (1a, 1b, 2a, etc). The HCV genome consists of a large open reading frame (ORF), flanked by highly structured 5’ and 3’ untranslated regions (UTRs). Both UTRs are conserved and control viral translation and replication. The HCV 5'-UTR contains an IRES that initiates cap-independent translation of the viral RNA. IRES-mediated translation of the HCV ORF yields a single polyprotein…

Advisors/Committee Members: Πατρινός, Γιώργος, Karamichali, Eirini, Τζάρτος, Σωκράτης, Πουλάς, Κωνσταντίνος.

Subjects/Keywords: Ηπατίτιδα C; Ρύθμιση της έκφρασης HCV IRES; 616.362 3; Hepatitis C; Regulation of HCV IRES

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Καραμιχάλη, �. (2014). Ρύθμιση της έκφρασης του IRES (εσωτερική θέση πρόσβασης του ριβοσώματος) του ιού της ηπατίτιδας C. (Doctoral Dissertation). University of Patras. Retrieved from http://hdl.handle.net/10889/8120

Chicago Manual of Style (16^th Edition):

Καραμιχάλη, Ειρήνη. “Ρύθμιση της έκφρασης του IRES (εσωτερική θέση πρόσβασης του ριβοσώματος) του ιού της ηπατίτιδας C.” 2014. Doctoral Dissertation, University of Patras. Accessed January 24, 2021. http://hdl.handle.net/10889/8120.

MLA Handbook (7^th Edition):

Καραμιχάλη, Ειρήνη. “Ρύθμιση της έκφρασης του IRES (εσωτερική θέση πρόσβασης του ριβοσώματος) του ιού της ηπατίτιδας C.” 2014. Web. 24 Jan 2021.

Vancouver:

Καραμιχάλη �. Ρύθμιση της έκφρασης του IRES (εσωτερική θέση πρόσβασης του ριβοσώματος) του ιού της ηπατίτιδας C. [Internet] [Doctoral dissertation]. University of Patras; 2014. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/10889/8120.

Council of Science Editors:

Καραμιχάλη �. Ρύθμιση της έκφρασης του IRES (εσωτερική θέση πρόσβασης του ριβοσώματος) του ιού της ηπατίτιδας C. [Doctoral Dissertation]. University of Patras; 2014. Available from: http://hdl.handle.net/10889/8120

Indian Institute of Science

12. Shwetha, S. Host-Pathogen Interactions in Hepatitis C Virus Infection : Deciphering the Role of Host Proteins and MicroRNAs.

Degree: PhD, Faculty of Science, 2018, Indian Institute of Science

URL: http://etd.iisc.ac.in/handle/2005/3858

► Host-pathogen interactions in Hepatitis C Virus infection: Deciphering the role of host proteins and microRNAs Hepatitis C virus (HCV) is a positive sense single stranded… (more)

▼ Host-pathogen interactions in Hepatitis C Virus infection: Deciphering the role of host proteins and microRNAs Hepatitis C virus (HCV) is a positive sense single stranded RNA virus belonging to the Hepacivirus genus of the Flaviviridae family. HCV genome consists of a single open reading frame flanked by highly structured 5‟ and 3‟ untranslated regions (UTRs) at both ends. Unlike cellular mRNAs, HCV RNA translation is independent of the cap structure and is mediated by an internal ribosomal entry site (IRES) present in the 5‟UTR. HCV replication begins with the synthesis of a complementary negative-strand RNA using the positive strand RNA genome as a template catalyzed by the NS5B RNA dependent RNA polymerase (RdRp). The de novo priming of HCV RNA synthesis by NS5B occurs at the very end of the 3‟UTR. The 3‟UTR is organized into highly structured regions namely the variable region, poly U/UC region and the 3‟X region. These regions contain cis-acting elements that determine the efficiency of viral replication. In addition, the interaction of trans-acting factors with the 3‟ UTR is also important for regulation of HCV replication. HCV 3‟UTR interacts with several cellular proteins such as the human La protein, polypyrimdine tract binding protein (PTB), poly (rC)-binding protein 2 (PCBP2) and Human antigen R (HuR). However, the molecular basis of regulation of viral replication by these proteins is not well understood. Many proteins that are hijacked by HCV as well as other cytoplasmic RNA viruses, such as La, PCBP2, HuR and PTB are RNA binding proteins (RBPs). They are involved in post transcriptional regulation of cellular gene expression. Thus the subversion of these proteins by the virus can affect their normal physiological functions. In addition to proteins, recent reports also describe the involvement of non-coding RNAs including microRNAs (miRNA) and long non coding RNAs (lncRNA) in HCV infection. miRNAs can either directly bind to the HCV genome and regulate its life cycle or indirectly modulate the expression of host proteins required by the virus. miRNAs that are differentially regulated in virus infected tissues or body fluids of infected patients can also serve as biomarkers for diagnosis of various stages of the disease. Hence, it was planned to study the role of host proteins and miRNAs in the HCV life cycle and pathogenesis to have novel insights into the biology of HCV infection. Riboproteomic studies have identified several host proteins that directly interact with the 5‟ and/or 3‟UTRs of the HCV RNA. One of the RNA binding proteins that predominantly interact with the 3‟UTR of HCV RNA was found to be HuR. In the present study, we have extensively characterized the interaction between HuR and HCV 3‟UTR and studied its functional implications in HCV life cycle along with other host factors. Characterizing the HCV 3’UTR–HuR interaction and its role in HCV replication HuR is a ubiquitously expressed member of the Hu family which shuttles between the nucleus and cytoplasm in response to stress. Whole… Advisors/Committee Members: Das, Saumitra (advisor).

Subjects/Keywords: Hepatitis C Virus Infection; Micro RNAs - HCV Infection; RNA Viruses; Hepatitis C Virus Replication; Hepatitis C Virus (HCV) RNA Binding; Viral Proteins; Host Proteins-HCV Infection; Hepatitis C Virus Pathogenesis; Hepatitis C Virus-Internal Ribosomal Entry Site (HCV-IRES) Mediated Translation; Hepatitis C Virus Diagnosis and Therapy; HCV-RNA Translation; HCV - miRNA; HCV Replication; HCV IRES; HCV Pathogenesis; Microbiology and Cell Biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Shwetha, S. (2018). Host-Pathogen Interactions in Hepatitis C Virus Infection : Deciphering the Role of Host Proteins and MicroRNAs. (Doctoral Dissertation). Indian Institute of Science. Retrieved from http://etd.iisc.ac.in/handle/2005/3858

Chicago Manual of Style (16^th Edition):

Shwetha, S. “Host-Pathogen Interactions in Hepatitis C Virus Infection : Deciphering the Role of Host Proteins and MicroRNAs.” 2018. Doctoral Dissertation, Indian Institute of Science. Accessed January 24, 2021. http://etd.iisc.ac.in/handle/2005/3858.

MLA Handbook (7^th Edition):

Shwetha, S. “Host-Pathogen Interactions in Hepatitis C Virus Infection : Deciphering the Role of Host Proteins and MicroRNAs.” 2018. Web. 24 Jan 2021.

Vancouver:

Shwetha S. Host-Pathogen Interactions in Hepatitis C Virus Infection : Deciphering the Role of Host Proteins and MicroRNAs. [Internet] [Doctoral dissertation]. Indian Institute of Science; 2018. [cited 2021 Jan 24]. Available from: http://etd.iisc.ac.in/handle/2005/3858.

Council of Science Editors:

Shwetha S. Host-Pathogen Interactions in Hepatitis C Virus Infection : Deciphering the Role of Host Proteins and MicroRNAs. [Doctoral Dissertation]. Indian Institute of Science; 2018. Available from: http://etd.iisc.ac.in/handle/2005/3858

13. Άιχερ, Στεφανή. Γενετική ποικιλομορφία του γονιδίου core του ιού της ηπατίτιδας C και μεταγραφική ρύθμιση.

Degree: 2013, University of Patras

URL: http://hdl.handle.net/10889/7295

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Η πολυλειτουργική πρωτεΐνη core του ιού της ηπατίτιδας C (HCV) εμπλέκεται στην ανάπτυξη ηπατοκυτταρικού καρκινώματος (HCC) που προκαλείται από τον ιό της ηπατίτιδας C, αλλά… (more)

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Η πολυλειτουργική πρωτεΐνη core του ιού της ηπατίτιδας C (HCV) εμπλέκεται στην ανάπτυξη ηπατοκυτταρικού καρκινώματος (HCC) που προκαλείται από τον ιό της ηπατίτιδας C, αλλά ο μηχανισμός με τον οποίο συμβαίνει αυτό δεν είναι κατανοητός. Η ενεργοποίηση του μονοπατιού Wnt/ β-κατενίνη, παίζει ένα σημαντικό ρόλο στην ανάπτυξη ηπατοκυττταρικού καρκίνου, και τροποποιείται από την πρωτεΐνη core του ιού της ηπατίτιδας C. Ο ιός της ηπατίτιδας C χαρακτηρίζεται από εκτεταμένη γενετική ποικιλομορφία και διαφορετικά κλινικά δείγματα διαφέρουν όσον αφορά την μολυσματικότητα τους και την παθογένεια που προκαλούν. Σκοπός αυτής της μελέτης είναι να καθοριστεί ο ρόλος της γενετικής ποικιλομορφίας της πρωτεΐνης HCV core στην ενεργοποίηση του μονοπατιού Wnt/ β-κατενίνη και να μελετηθεί ο μοριακός μηχανισμός με τον οποίο η πρωτεΐνη HCV core ρυθμίζει την ενεργοποίηση αυτή. Η ενεργότητα του μονοπατιού Wnt/β-κατενίνη μελετήθηκε σε HEK 293T και Huh 7.5 κυτταρικές σειρές που εκφράζουν παροδικά τις πρωτεΐνες core των γενοτύπων 1a, 1b, 3a, 4a, 4f και από ένα μοναδικό δείγμα του γενοτύπου 1a που προέρχεται από έναν ασθενή από την Καμπότζη (1aCam). Μελέτες βασισμένες στη μέτρηση ενεργότητας της λουσιφεράσης, Western blot ανάλυση και qPCR, χρησιμοποιήθηκαν για την μέτρηση των επιπέδων έκφρασης γονιδίων και πρωτεϊνών. Βρέθηκε ότι η HCV core πρωτεΐνη ρυθμίζει θετικά την μεσολαβούμενη από τη β-κατενίνη Tcf-εξαρτώμενη ενεργότητα της λουσιφεράσης σε ένα γενοτυπο-εξαρτώμενο τρόπο. Σε συμφωνά με τα αποτελέσματα αυτά βρέθηκε ότι η πρωτεΐνη HCV core σταθεροποίει τα επίπεδα της β-κατενίνης, τόσο σε παροδικά μετασχηματισμένα κύτταρα, όσο και σε κύτταρα που μολύνονται με βακουλοϊούς που εκφράσουν τις πρωτεΐνες core των υποτύπων 4a και 4f. Τέλος, βρέθηκε ότι η πρωτεΐνη HCV core συμβάλει στην θετική ρύθμιση των γονιδίων c-myc, αξίνης και Tbx3, τα οποία είναι καθοδικά γονίδια στόχοι του μονοπατιού Wnt/β-κατενίνη και εμπλέκονται στην ανάπτυξη ηπατοκυτταρικού καρκινώματος. Συμπερασματικά, οι πρωτεΐνες HCV core διαφορετικών γενοτύπων του ιού ρυθμίζουν διαφορετικά το μονοπάτι σηματοδότησης Wnt/β-κατενίνη και η διαφορετική αυτή ρύθμιση μπορεί να σχετίζεται με ικανότητα των διαφόρων γενοτύπων του ιού της ηπατίτιδας C να επάγουν την ανάπτυξη ηπατοκυτταρικού καρκινώματος.

The multifunctional HCV core protein is implicated in the development of hepatocellular carcinoma (HCC) caused by HCV infection, but the underlying mechanism is not fully understood. Activation of the Wnt/ β-catenin pathway plays a major role in HCC and is modulated by the HCV core protein. HCV is characterized by extensive genetic diversity and different clinical isolates do vary in their infectivity and pathogenesis mainly due to variations in the structure/function relationships of individual viral proteins. The aim of this study is to determine the possible influence of genetic variability in HCV core protein in enhancing the Wnt/ β-catenin signaling activity and to elucidate the molecular mechanisms by which HCV core modulates activation of β-catenin. The Wnt/β-catenin activity was investigated…

Advisors/Committee Members: Πουλάς, Κωνσταντίνος, Aicher, Stephanie, Γεώργιος, Πατρινός.

Subjects/Keywords: Ηπατίτιδα C; Ιός της ηπατίτιδας C (HCV); Πρωτεΐνη core; 616.362 3; Hepatitis C; Hepatitis C virus (HCV); Core protein

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Άιχερ, �. (2013). Γενετική ποικιλομορφία του γονιδίου core του ιού της ηπατίτιδας C και μεταγραφική ρύθμιση. (Masters Thesis). University of Patras. Retrieved from http://hdl.handle.net/10889/7295

Chicago Manual of Style (16^th Edition):

Άιχερ, Στεφανή. “Γενετική ποικιλομορφία του γονιδίου core του ιού της ηπατίτιδας C και μεταγραφική ρύθμιση.” 2013. Masters Thesis, University of Patras. Accessed January 24, 2021. http://hdl.handle.net/10889/7295.

MLA Handbook (7^th Edition):

Άιχερ, Στεφανή. “Γενετική ποικιλομορφία του γονιδίου core του ιού της ηπατίτιδας C και μεταγραφική ρύθμιση.” 2013. Web. 24 Jan 2021.

Vancouver:

Άιχερ �. Γενετική ποικιλομορφία του γονιδίου core του ιού της ηπατίτιδας C και μεταγραφική ρύθμιση. [Internet] [Masters thesis]. University of Patras; 2013. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/10889/7295.

Council of Science Editors:

Άιχερ �. Γενετική ποικιλομορφία του γονιδίου core του ιού της ηπατίτιδας C και μεταγραφική ρύθμιση. [Masters Thesis]. University of Patras; 2013. Available from: http://hdl.handle.net/10889/7295

14. Da Costa, Daniel. Study of cell host factors involved in Hepatitis C virus tropism : Etude des facteurs cellulaires de l'hôte impliqués dans le tropisme du virus de l'hépatite C.

Degree: Docteur es, Virologie : aspects moléculaires et médicaux, 2012, Université de Strasbourg

URL: http://www.theses.fr/2012STRAJ071

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Le virus de l’hépatite C (HCV) est un problème majeur de santé publique. Le développement de nouveaux traitements pour lutter contre le HCV a été… (more)

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Le virus de l’hépatite C (HCV) est un problème majeur de santé publique. Le développement de nouveaux traitements pour lutter contre le HCV a été ralenti par l’absence de modèles d’études in vitro et in vivo convenables. Le but de mon travail de thèse a été, dans un premier temps, de caractériser les facteurs déterminant le tropisme hépatique du HCV. En exprimant des facteurs clés dans une lignée cellulaire humaine non-hépatocytaire, nous avons reconstitué in fine l’ensemble du cycle viral dans ces cellules. L’entrée du virus dans la cellule hôte fait intervenir différents récepteurs d’entrée dont CD81, occludin (OCLN), claudin-1 (CLDN1) et scavenger receptor class B type I (SR-BI). L’expression de ces quatre récepteurs sur cette lignée la rend hautement permissive à l’entrée du virus, mais ne permet pas de rétablir la réplication du virus. L’expression du micro-ARN 122, un micro-RNA important pour l’infection du HCV, dans les cellules exprimant les quatre récepteurs, restaure une forte réplication de l’ARN viral mais ne permet pas de détecter une production de particules infectieuses. L’expression de l’apolipoprotein E (apoE), jouant un rôle primordial dans l’assemblage et la sécrétion, rétablis cette dernière étape du cycle viral du HCV dans la lignée cellulaire humaine non-hépatocytaire. Dans un second temps, j’ai utilisé la stratégie, précédemment établie, pour étudier la spécificité d’espèce de l’infection du HCV dans plusieurs lignées hépatocytaires murines. Nous avons pu rendre ces cellules permissives à l’entrée du HCV et pu détecter une très faible réplication. L’ensemble de mes travaux apportent de nouvelles informations sur la compréhension des facteurs clés nécessaire au cycle viral du HCV dans des cellules murines et humaines.

Hepatitis C virus (HCV) is a global health burden. The development of new therapeutics to treat HCV infection has been hampered by the lack of convenient in vitro and in vivo model systems. The goal of my PhD work was, in a first time, to characterize the factors determining the hepatotropism of HCV. By expressing key factors within a non-hepatic cell line, we reconstituted in fine the full HCV life cycle in those cells. Virus entry into the host cell requires different entry factors which are CD81, occludin (OCLN), claudin-1 (CLDN1) and the scavenger receptor class B type I (SR-BI). The expression of these four factors in this cell line renders it highly permissive to viral entry, but does not allow restoring replication of the virus. The expression of miR-122, a micro-RNA important for HCV infection, into the cell lines expressing the four HCV entry factors restore a strong replication of the HCV RNA but does not allow detecting infectious viral particle production. Further expression of the apolipoprotein E (apoE), which plays a critical role in the assembly and release process, restore the last step of the HCV life cycle in a non-hepatic cell line. In a second part of my PhD, I have used the previously developed strategy to study the species specificity of HCV infection…

Advisors/Committee Members: Baumert, Thomas (thesis director).

Subjects/Keywords: Intéraction virus-hôte; Tropisme du HCV; Spécificité d’espèce du HCV; Entrée du HCV; Replication du HCV; Assemblage du HCV et modèle murin du HCV; Hepatitis C virus infection; Virus-host interaction; HCV tropism; HCV species specificity; HCV entry; HCV replication; HCV assembly and HCV mouse model; 579.2; 616.91; 572.8

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Da Costa, D. (2012). Study of cell host factors involved in Hepatitis C virus tropism : Etude des facteurs cellulaires de l'hôte impliqués dans le tropisme du virus de l'hépatite C. (Doctoral Dissertation). Université de Strasbourg. Retrieved from http://www.theses.fr/2012STRAJ071

Chicago Manual of Style (16^th Edition):

Da Costa, Daniel. “Study of cell host factors involved in Hepatitis C virus tropism : Etude des facteurs cellulaires de l'hôte impliqués dans le tropisme du virus de l'hépatite C.” 2012. Doctoral Dissertation, Université de Strasbourg. Accessed January 24, 2021. http://www.theses.fr/2012STRAJ071.

MLA Handbook (7^th Edition):

Da Costa, Daniel. “Study of cell host factors involved in Hepatitis C virus tropism : Etude des facteurs cellulaires de l'hôte impliqués dans le tropisme du virus de l'hépatite C.” 2012. Web. 24 Jan 2021.

Vancouver:

Da Costa D. Study of cell host factors involved in Hepatitis C virus tropism : Etude des facteurs cellulaires de l'hôte impliqués dans le tropisme du virus de l'hépatite C. [Internet] [Doctoral dissertation]. Université de Strasbourg; 2012. [cited 2021 Jan 24]. Available from: http://www.theses.fr/2012STRAJ071.

Council of Science Editors:

Da Costa D. Study of cell host factors involved in Hepatitis C virus tropism : Etude des facteurs cellulaires de l'hôte impliqués dans le tropisme du virus de l'hépatite C. [Doctoral Dissertation]. Université de Strasbourg; 2012. Available from: http://www.theses.fr/2012STRAJ071

Johannes Gutenberg Universität Mainz

15. Kehl, Torben Michael. Analyse von Pathomechanismen im Rahmen der Hepatitis-C-Infektion.

Degree: 2011, Johannes Gutenberg Universität Mainz

URL: http://ubm.opus.hbz-nrw.de/volltexte/2011/2805/

► Das Hepatitis C Virus stellt mit weltweit 170 Millionen infizierten Menschen ein großes gesundheitliches Problem dar. Zwar sind in den letzten Jahren deutliche Fortschritte in… (more)

Subjects/Keywords: Hepatitis C, Pathomechanismen, Adenovirale Vektoren, Apoptose, HCV-Core, HCV-NS5a; hepatitis c, pathomechanism, adenoviral vector, apoptosis, hcv-core, hcv-ns5a; Medical sciences Medicine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kehl, T. M. (2011). Analyse von Pathomechanismen im Rahmen der Hepatitis-C-Infektion. (Doctoral Dissertation). Johannes Gutenberg Universität Mainz. Retrieved from http://ubm.opus.hbz-nrw.de/volltexte/2011/2805/

Chicago Manual of Style (16^th Edition):

Kehl, Torben Michael. “Analyse von Pathomechanismen im Rahmen der Hepatitis-C-Infektion.” 2011. Doctoral Dissertation, Johannes Gutenberg Universität Mainz. Accessed January 24, 2021. http://ubm.opus.hbz-nrw.de/volltexte/2011/2805/.

MLA Handbook (7^th Edition):

Kehl, Torben Michael. “Analyse von Pathomechanismen im Rahmen der Hepatitis-C-Infektion.” 2011. Web. 24 Jan 2021.

Vancouver:

Kehl TM. Analyse von Pathomechanismen im Rahmen der Hepatitis-C-Infektion. [Internet] [Doctoral dissertation]. Johannes Gutenberg Universität Mainz; 2011. [cited 2021 Jan 24]. Available from: http://ubm.opus.hbz-nrw.de/volltexte/2011/2805/.

Council of Science Editors:

Kehl TM. Analyse von Pathomechanismen im Rahmen der Hepatitis-C-Infektion. [Doctoral Dissertation]. Johannes Gutenberg Universität Mainz; 2011. Available from: http://ubm.opus.hbz-nrw.de/volltexte/2011/2805/

University of Illinois – Chicago

16. Martin, Danyelle N. Identification and Characterization of Transferrin Receptor 1 and Other Novel HCV Entry Factors.

Degree: 2012, University of Illinois – Chicago

URL: http://hdl.handle.net/10027/9327

► Hepatitis C virus (HCV) is a liver tropic virus spread via blood. Because the majority of infections fail to clear, approximately 80% of patients develop… (more)

Subjects/Keywords: HCV; viral entry; TfR1; HCV therapeutics; iron overload; transferrin receptor 1; hepatitis C virus

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Martin, D. N. (2012). Identification and Characterization of Transferrin Receptor 1 and Other Novel HCV Entry Factors. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/9327

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Martin, Danyelle N. “Identification and Characterization of Transferrin Receptor 1 and Other Novel HCV Entry Factors.” 2012. Thesis, University of Illinois – Chicago. Accessed January 24, 2021. http://hdl.handle.net/10027/9327.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Martin, Danyelle N. “Identification and Characterization of Transferrin Receptor 1 and Other Novel HCV Entry Factors.” 2012. Web. 24 Jan 2021.

Vancouver:

Martin DN. Identification and Characterization of Transferrin Receptor 1 and Other Novel HCV Entry Factors. [Internet] [Thesis]. University of Illinois – Chicago; 2012. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/10027/9327.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Martin DN. Identification and Characterization of Transferrin Receptor 1 and Other Novel HCV Entry Factors. [Thesis]. University of Illinois – Chicago; 2012. Available from: http://hdl.handle.net/10027/9327

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

17. Κουντουράς, Δημήτριος. Επιδημιολογικά και κλινικά χαρακτηριστικά, φυσική πορεία και θεραπευτικές παρεμβάσεις σε ενηλίκους ασθενείς με ομόζυγο β- μεσογειακή αναιμία και χρόνια HCV λοίμωξη.

Degree: 2012, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/29688

► ΣΥΜΠΕΡΑΣΜΑΤΑΗ παράταση της επιβίωσης των ασθενών με θαλασσαιμία, απαιτεί την εκ νέουμελέτη της ηπατικής νόσου, όπως αυτή εξελίσσεται πλέον σε ένα μοναδικόπληθυσμό ενηλίκων και όχι… (more)

▼ ΣΥΜΠΕΡΑΣΜΑΤΑΗ παράταση της επιβίωσης των ασθενών με θαλασσαιμία, απαιτεί την εκ νέουμελέτη της ηπατικής νόσου, όπως αυτή εξελίσσεται πλέον σε ένα μοναδικόπληθυσμό ενηλίκων και όχι παιδιών. Με μέσο την αποτελεσματική αποσιδήρωσηκαι χωρίς τη βαρειά εναπόθεση σιδήρου του παρελθόντος, ο ρυθμός εξέλιξης είναιάγνωστος, παρά τα εμφανή αποτελέσματα της μακροχρόνιας νόσησης παράλληλαμε τη φυσική εξέλιξη της ενηλικίωσης.Τόσο η εναπόθεση σιδήρου, όσο και η παρουσία της λοίμωξης από τον ιό τηςηπατίτιδας C, εξακολουθούν να διαδραματίζουν πρωταγωνιστικό ρόλο, χωρίς νααποκλείεται, κυρίως στο παρελθόν, η συνεργική δράση της συμφορητικήςκαρδιακής ανεπάρκειας.Η συμβολή ενός εκάστου αυτών των παραγόντων στο τελικό αποτέλεσμα τηςηπατικής νόσου προσδιορίζει και τις θεραπευτικές προτεραιότητες.Είναι ενδιαφέρον επίσης ότι, παρά τα αναμενόμενα, η παράταση της επιβίωσηςσυνοδεύεται από σταθεροποίηση της ηπατικής νόσου, η οποία σε μερικές μόνοπεριπτώσεις επιδεινώνεται στην πορεία του χρόνου προς ηπατική νόσο τελικούσταδίου.Συμπερασματικά λοιπόν, με βάση τα αποτελέσματα της συγκεκριμένης ερευνητικήςκλινικής μελέτης, η ηπατική ίνωση στους ασθενείς με ΟΒΜ, είναι το αποτέλεσμα τηςεπίδρασης πολλαπλών παραγόντων.Μεμονωμένα η ηπατίτιδα C δεν διαδραματίζει τον πρωτεύοντα ρόλο, χωρίς όμωςκαι να είναι σαφής η συμπεριφορά της λοίμωξης σε θαλασσαιμικούς ασθενείς μεφυσιολογικό φορτίο σιδήρου.Η υπερφόρτωση με σίδηρο αποτελεί τον κρίσιμο παράγοντα, καθώς η ίνωση είναιπαρούσα σε όλους τους βαθμούς αιμοσιδήρωσης, ακόμη και στον ελάχιστο.Επίσης, η ίνωση παραμένει σχετικά σταθερή στο βάθος του χρόνου, ανεξάρτητααπό την αυξανόμενη ηπατική αιμοσιδήρωση ή την παρουσία HCV λοίμωξης, ενώ ηεξέλιξή της είναι άμεσα συναρτώμενη με το βαθμό της ηπατικής βλάβης (το μοτίβοτης ALT).

Subjects/Keywords: Θαλασσαιμία; Σίδηρος; Ηπατίτιδα C, Ιός (HCV); Ήπαρ; Ίνωση; Thalassemia; Iron; HCV; Liver; Fibrosis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Κουντουράς, . �. (2012). Επιδημιολογικά και κλινικά χαρακτηριστικά, φυσική πορεία και θεραπευτικές παρεμβάσεις σε ενηλίκους ασθενείς με ομόζυγο β- μεσογειακή αναιμία και χρόνια HCV λοίμωξη. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/29688

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Κουντουράς, Δημήτριος. “Επιδημιολογικά και κλινικά χαρακτηριστικά, φυσική πορεία και θεραπευτικές παρεμβάσεις σε ενηλίκους ασθενείς με ομόζυγο β- μεσογειακή αναιμία και χρόνια HCV λοίμωξη.” 2012. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed January 24, 2021. http://hdl.handle.net/10442/hedi/29688.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Κουντουράς, Δημήτριος. “Επιδημιολογικά και κλινικά χαρακτηριστικά, φυσική πορεία και θεραπευτικές παρεμβάσεις σε ενηλίκους ασθενείς με ομόζυγο β- μεσογειακή αναιμία και χρόνια HCV λοίμωξη.” 2012. Web. 24 Jan 2021.

Vancouver:

Κουντουράς �. Επιδημιολογικά και κλινικά χαρακτηριστικά, φυσική πορεία και θεραπευτικές παρεμβάσεις σε ενηλίκους ασθενείς με ομόζυγο β- μεσογειακή αναιμία και χρόνια HCV λοίμωξη. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2012. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/10442/hedi/29688.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Κουντουράς �. Επιδημιολογικά και κλινικά χαρακτηριστικά, φυσική πορεία και θεραπευτικές παρεμβάσεις σε ενηλίκους ασθενείς με ομόζυγο β- μεσογειακή αναιμία και χρόνια HCV λοίμωξη. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2012. Available from: http://hdl.handle.net/10442/hedi/29688

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Sydney

18. Mitchell, Sandra. What Do We Know About Women’s Experiences of Living With Hepatitis C? An Analysis of Canadian Women's Journey with Hepatitis C Care .

Degree: 2017, University of Sydney

URL: http://hdl.handle.net/2123/17306

► Background: The Hepatitis C virus (HCV) is a blood-borne infection affecting an estimated 170 million people worldwide including approximately 250,000 Canadians. Untreated HCV can contribute… (more)

▼ Background: The Hepatitis C virus (HCV) is a blood-borne infection affecting an estimated 170 million people worldwide including approximately 250,000 Canadians. Untreated HCV can contribute to significant morbidity and mortality. Despite the benefits of HCV care, there continues to be significant gaps in the uptake of services. Purpose: This thesis explored Canadian women’s experiences of the journey with HCV care from the perspective of the women, in order to promote care engagement, improve patient-provider relationships and deliver services that meet women’s needs. Methods: This study, inspired by grounded theory techniques, explored women’s experience of living with HCV and factors influencing their journey with care. Purposive and theoretical sampling across three Canadian provinces generated interviews with 25 women. Results: Three concepts were central to understanding women’s journey with HCV care: 1) The point of diagnosis shaped women’s journey with care through a) their preparedness for a positive diagnosis, and b) the information/health education they received; 2) Women faced complex barriers to care - (a) information provision, b) family and caregiver responsibilities, c) relationship with healthcare provider, d) active substance use and e) stigma and discrimination - but often showed inventiveness and determination to overcome them; 3) Women saw their decision to attend for HCV care as prompted by a) immediate crisis, b) gradual sequence of awareness, or c) medical intervention. Conclusion: The development of effective interventions and integrated models of care requires an understanding of the complex factors that shape women’s journey with HCV care. Improving women’s journey with HCV care will enhance their access to the new treatment regimes. Recommendations: A National HCV Strategy and comprehensive guidelines for care, treatment and prevention; HCV education throughout the healthcare system; and equitable and accessible healthcare for all women living with HCV.

Subjects/Keywords: Hepatitis C; Women living with HCV journey with care; Journey with care; Women and HCV

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mitchell, S. (2017). What Do We Know About Women’s Experiences of Living With Hepatitis C? An Analysis of Canadian Women's Journey with Hepatitis C Care . (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/17306

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mitchell, Sandra. “What Do We Know About Women’s Experiences of Living With Hepatitis C? An Analysis of Canadian Women's Journey with Hepatitis C Care .” 2017. Thesis, University of Sydney. Accessed January 24, 2021. http://hdl.handle.net/2123/17306.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mitchell, Sandra. “What Do We Know About Women’s Experiences of Living With Hepatitis C? An Analysis of Canadian Women's Journey with Hepatitis C Care .” 2017. Web. 24 Jan 2021.

Vancouver:

Mitchell S. What Do We Know About Women’s Experiences of Living With Hepatitis C? An Analysis of Canadian Women's Journey with Hepatitis C Care . [Internet] [Thesis]. University of Sydney; 2017. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2123/17306.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mitchell S. What Do We Know About Women’s Experiences of Living With Hepatitis C? An Analysis of Canadian Women's Journey with Hepatitis C Care . [Thesis]. University of Sydney; 2017. Available from: http://hdl.handle.net/2123/17306

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

19. Lévy, Pierre. Hepatitis C virus-induced reprogramming of glutamine metabolism : Reprogrammation du métabolisme de la glutamine par le virus de l'hépatite C.

Degree: Docteur es, Biologie, 2014, Université Claude Bernard – Lyon I

URL: http://www.theses.fr/2014LYO10328

►

L'hépatite C chronique est une des étiologies principales du carcinome hépatocellulaire. En revanche, les mécanismes de tumorigenèse sont peu connus. Récemment, plusieurs modifications du métabolisme… (more)

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L'hépatite C chronique est une des étiologies principales du carcinome hépatocellulaire. En revanche, les mécanismes de tumorigenèse sont peu connus. Récemment, plusieurs modifications du métabolisme du glucose ont été décrites dans les cellules infectées par le HCV. Celles-ci évoquent les reprogrammations métaboliques mises en place dans les cellules cancéreuses. L'effet Warburg, ou glycolyse aérobie, est une des caractéristiques principales des cellules tumorales. Ce phénomène permet d'assurer une production énergétique ainsi qu'un stock en précurseurs de macromolécules suffisants pour permettre la prolifération. Par ailleurs, en complément de l'utilisation de glucose, les cellules tumorales deviennent dépendantes de la métabolisation de la glutamine pour alimenter leur métabolisme énergétique et les différentes voies de biosynthèse. Mes travaux de thèse ont porté sur l'étude des changements métaboliques caractéristiques des cellules cancéreuses, et plus précisément sur le métabolisme de la glutamine, dans les cellules infectées par le HCV. Dans le modèle de culture cellulaire HCVcc, une activation de l'utilisation de la glutamine par le virus a pu être mise en évidence. L'infection par HCV entraine une augmentation du facteur de transcription MYC, de plusieurs transporteurs de glutamine ainsi que de la glutaminase, l'enzyme limitante de la glutaminolyse. De façon intéressante, ces changements semblent survenir également chez les personnes chroniquement infectés par le virus, comme le suggère l'analyse des biopsies de patients. Ces altérations métaboliques pourraient participer à la mise en place d'un environnement favorable au développement tumoral

Chronic infection with hepatitis C virus (HCV) is one of the main etiologies of hepatocellular carcinoma (HCC). However, mechanisms of HCV-related tumorigenesis are ill-defined. Recent literature data suggest that HCV infection may reprogram glucose metabolism in a cancerlike fashion. The Warburg effect, or aerobic glycolysis, is a hallmark of cancer. Activation of this pathway allows tumor cells to sustain high rates of energy production and provide sufficient biosynthetic precursors for proliferation. Likewise, the induction of similar metabolic alterations may favor HCV multiplication through the rapid production of nucleotides, amino acids and lipids. To complement aerobic glycolysis, tumor cells become frequently dependent on glutamine. The partial oxidation of glutamine through the glutaminolytic pathway can fuel their energy metabolism and several anabolic pathways. However, the role of glutamine metabolism in HCV life cycle has not been documented so far. I focused my PhD research project on the characterization of metabolic alterations triggered by HCV. In particular, I evaluated the occurrence of distinctive features of tumor cell metabolism in HCVinfected cells, with a specific attention on glutamine utilization. In the HCVcc cell culture model, I report the induction of a metabolic reprogramming towards higher rates of glutaminolysis upon HCV infection.…

Advisors/Committee Members: Bartosch, Birke (thesis director).

Subjects/Keywords: Virus de l’hépatite C (HCV); Cancer; Métabolisme; Glucose; Glutamine; Hepatitis C virus (HCV); Cancer; Metabolism; Glucose; Glutamine; 572

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lévy, P. (2014). Hepatitis C virus-induced reprogramming of glutamine metabolism : Reprogrammation du métabolisme de la glutamine par le virus de l'hépatite C. (Doctoral Dissertation). Université Claude Bernard – Lyon I. Retrieved from http://www.theses.fr/2014LYO10328

Chicago Manual of Style (16^th Edition):

Lévy, Pierre. “Hepatitis C virus-induced reprogramming of glutamine metabolism : Reprogrammation du métabolisme de la glutamine par le virus de l'hépatite C.” 2014. Doctoral Dissertation, Université Claude Bernard – Lyon I. Accessed January 24, 2021. http://www.theses.fr/2014LYO10328.

MLA Handbook (7^th Edition):

Lévy, Pierre. “Hepatitis C virus-induced reprogramming of glutamine metabolism : Reprogrammation du métabolisme de la glutamine par le virus de l'hépatite C.” 2014. Web. 24 Jan 2021.

Vancouver:

Lévy P. Hepatitis C virus-induced reprogramming of glutamine metabolism : Reprogrammation du métabolisme de la glutamine par le virus de l'hépatite C. [Internet] [Doctoral dissertation]. Université Claude Bernard – Lyon I; 2014. [cited 2021 Jan 24]. Available from: http://www.theses.fr/2014LYO10328.

Council of Science Editors:

Lévy P. Hepatitis C virus-induced reprogramming of glutamine metabolism : Reprogrammation du métabolisme de la glutamine par le virus de l'hépatite C. [Doctoral Dissertation]. Université Claude Bernard – Lyon I; 2014. Available from: http://www.theses.fr/2014LYO10328

20. Jovanović-Ćupić Snežana. Conserved properties of hepatitis C genotype 1b proteinsas prognostic markers of response to pegylated interferon and ribavirin combination therapy.

Degree: PhD, Biology, 2012, University of Belgrade

URL: http://dx.doi.org/10.2298/BG20120710JOVANOVICCUPIC ; http://eteze.bg.ac.rs/application/showtheses?thesesId=35 ; https://fedorabg.bg.ac.rs/fedora/get/o:2323/bdef:Content/get ; http://vbs.rs/scripts/cobiss?command=SEARCH&base=99999&select=ID=1024532402

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Background: Hepatitis C virus (HCV) infection is a major and rising global health problem, affecting about 170 million people worldwide, according to WHO data, and… (more)

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Background: Hepatitis C virus (HCV) infection is a major and rising global health problem, affecting about 170 million people worldwide, according to WHO data, and often leading to chronic liver disease and cirrhosis. The current standard therapy for chronic HCV infection with pegylated interferon combined with ribavirin in patients with the genotype 1 infection, the most frequent genotype in the USA, Western Europe and Serbia, accompanied by numerous side effects, leads to a successful outcome in only about 50% of individuals. Therefore, simple and accurate prediction of hepatitis C treatment response is of great benefit to patients and clinicians. Methods: Identification of the conserved information of the HCV proteins that correlate with the combination therapy outcome was performed by bioinformatics analysis. Plasma samples of 48 chronic HCV patients from Serbia were classified according to the outcome of therapy. To determine primary structure of HCV proteins classical methods of molecular biology: reverse transcription and polymerase chain reaction (Rt-PCR), the absolute quantification-Real Time PCR and DNA sequencing were applied. Results: Among the HCV proteins that we have analyzed the informational property of the p7 of HCV genotype 1b was best related to the therapy outcome. Findings obtained from analyzing sequences 48 patients collected from Serbia were in perfect agreement with proposed bioinformatics criterion. Special attention was paid to optimization experimental protocols and forming homogeneous groups of patients regarding HCV genotype (type and subtype) and therapy response. Conclusions: On the basis of the results in the present study, a simple bioinformatics criterion that could be useful in assessment of the response of HCV-infected patients to the combination therapy has been proposed.

Uvod: Infekcija virusom hepatitisa C (HCV) predstavlja značajan globalni zdravstveni problem koji često vodi ka hroničnoj bolesti jetre i cirozi. Prema podacima Svetske zdravstvene organizacije registrovano je preko 170 miliona ljudi inficiranih virusom HCV. Sadašnja standardna terapija hroničnog HCV-a kod pacijenata inficiranih genotipom 1b, koji predstavlja najčešći genotip kako u SAD, zapadnoj Evropi i Srbiji, se sastoji od pegilovanog interferona u kombinaciji sa ribavirinom. Međutim, kombinovana terapija je praćena brojnim neželjenim efektima i dovodi do stabilnog virusološkog odgovora samo kod 50% pacijenata inficiranih genotipom 1. Stoga bi jednostavan i pouzdan test, koji bi pre početka terapije mogao da predvidi virusološki odgovor, bio od velike koristi u kliničkoj praksi. Metode: Identifikacija konzervirane informacije sadržane u proteinima HCV koja korelira sa odgovorom na standardnu kombinovanu terapiju rađena je bioinformatičkom analizom. Uzorci plazme 48 pacijenata sa hroničnom infekcijom HCV, genotipa 1b su klasifikovani u odnosu na odgovor na kombinovanu terapiju. Za određivanje primarne strukture proteina HCV primenjene su klasične metode molekularne…

Subjects/Keywords: Hepatitis C virus (HCV); Bioinformatics; Informational spectrum method; Protein sequence; Hepatitis C virus (HCV); bioinformatika; metod informacionih spektara; proteinske sekvence

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Snežana, J. (2012). Conserved properties of hepatitis C genotype 1b proteinsas prognostic markers of response to pegylated interferon and ribavirin combination therapy. (Doctoral Dissertation). University of Belgrade. Retrieved from http://dx.doi.org/10.2298/BG20120710JOVANOVICCUPIC ; http://eteze.bg.ac.rs/application/showtheses?thesesId=35 ; https://fedorabg.bg.ac.rs/fedora/get/o:2323/bdef:Content/get ; http://vbs.rs/scripts/cobiss?command=SEARCH&base=99999&select=ID=1024532402

Chicago Manual of Style (16^th Edition):

Snežana, Jovanović-Ćupić. “Conserved properties of hepatitis C genotype 1b proteinsas prognostic markers of response to pegylated interferon and ribavirin combination therapy.” 2012. Doctoral Dissertation, University of Belgrade. Accessed January 24, 2021. http://dx.doi.org/10.2298/BG20120710JOVANOVICCUPIC ; http://eteze.bg.ac.rs/application/showtheses?thesesId=35 ; https://fedorabg.bg.ac.rs/fedora/get/o:2323/bdef:Content/get ; http://vbs.rs/scripts/cobiss?command=SEARCH&base=99999&select=ID=1024532402.

MLA Handbook (7^th Edition):

Snežana, Jovanović-Ćupić. “Conserved properties of hepatitis C genotype 1b proteinsas prognostic markers of response to pegylated interferon and ribavirin combination therapy.” 2012. Web. 24 Jan 2021.

Vancouver:

Snežana J. Conserved properties of hepatitis C genotype 1b proteinsas prognostic markers of response to pegylated interferon and ribavirin combination therapy. [Internet] [Doctoral dissertation]. University of Belgrade; 2012. [cited 2021 Jan 24]. Available from: http://dx.doi.org/10.2298/BG20120710JOVANOVICCUPIC ; http://eteze.bg.ac.rs/application/showtheses?thesesId=35 ; https://fedorabg.bg.ac.rs/fedora/get/o:2323/bdef:Content/get ; http://vbs.rs/scripts/cobiss?command=SEARCH&base=99999&select=ID=1024532402.

Council of Science Editors:

Snežana J. Conserved properties of hepatitis C genotype 1b proteinsas prognostic markers of response to pegylated interferon and ribavirin combination therapy. [Doctoral Dissertation]. University of Belgrade; 2012. Available from: http://dx.doi.org/10.2298/BG20120710JOVANOVICCUPIC ; http://eteze.bg.ac.rs/application/showtheses?thesesId=35 ; https://fedorabg.bg.ac.rs/fedora/get/o:2323/bdef:Content/get ; http://vbs.rs/scripts/cobiss?command=SEARCH&base=99999&select=ID=1024532402

21. Andrade, Midiã Barbosa Pimentel de. Caracterização epidemiológica, virológica e imunológica de voluntários anti-HCV reativos do município de Iranduba, região metropolitana de Manaus.

Degree: 2013, Universidade Federal do Amazonas

URL: http://tede.ufam.edu.br/handle/tede/2626

►

A hepatite C é uma doença caracterizada pela inflamação do fígado, sendo o vírus HCV transmitido, principalmente, através de sangue contaminado. Estima-se que 170 milhões… (more)

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A hepatite C é uma doença caracterizada pela inflamação do fígado, sendo o vírus HCV transmitido, principalmente, através de sangue contaminado. Estima-se que 170 milhões de pessoas, cerca de 3% da população mundial, sejam portadores de hepatite C. Ao contrário dos demais vírus que causam hepatite, o HCV não gera resposta imunológica adequada no organismo, o que faz com que 15 a 20% das pessoas infectadas curem espontâneamente, enquanto 80% evoluem para cronificação da doença. No Brasil ocorre grande variação na prevalência da infecção, isso se deve principalmente, as distintas características epidemiológicas entre as populações e a região geográfica estudada, além de restritas informações sobre a doença. Não existe vacina contra a hepatite C, assim o diagnóstico precoce amplia a eficácia do tratamento. O objetivo deste trabalho consistiu em caracterizar o perfil epidemiológico, virológico e imunológico anti-HCV na população geral, residente no município de Iranduba, região metropolitana de Manaus. Participaram da pesquisa 700 voluntários. Foi detectado um caso positivo no teste rápido anti-HCV, apesar da presença dos clássicos fatores de risco para a infecção nos voluntários soronegativos. A realização do teste molecular, reação em cadeia da polimerase, confirmou o diagnóstico da infecção e através da análise filogenética da região NS5B do genoma viral foi constatado o genótipo 2b. Dentres as citocinas quantificadas, no caso anti-HCV positivo, IL-2, IL-6, IL-10, TNF-α e IL-17A foram mais prevalentes. O estudo apontou soroprevalência anti-HCV de 0,14%, classificando o município de Iranduba como local de baixa endemicidade para o HCV e reforçando a caracterização heterogênea do Amazonas para a hepatite C.

Hepatitis C is a disease characterized by the inflammation of the liver with HCV transmitted primarily through infected blood. It is estimated that 170 million people, about 3% of the world population, is infected with hepatitis C. Unlike other viruses that cause hepatitis, HCV does not generate adequate immune response in the body, which makes 80% of the infected to evolve into chronicity, whereas 15 to 20% of infected people heal spontaneously. In Brazil there is a significant variation in the prevalence of infection, the main cause of this epidemiological distinction is the characteristics differences between populations and geographic that each region studied has, and the limited epidemiological data about the disease. There is no vaccine against hepatitis C virus, thus early diagnosis increases the effectiveness of treatment. The aim of this study was to characterize the epidemiological, virological and immunological anti-HCV general population living in the city of Iranduba, metropolitan region of Manaus. Where were evaluated 700 volunteers and it was detected a positive case, using a rapid anti-HCV test, despite the presence of classical risk factors for infection in the seronegative volunteers. The realization of a molecular test, the polymerase chain reaction, confirmed the diagnosis of infection and by…

Advisors/Committee Members: Rocha, Cristina Melo, CPF:45599670244.

Subjects/Keywords: Epidemiologia; Diagnóstico para HCV; Transmissão da hepatite C; Iranduba; Epidemiology; Diagnosis for HCV; Hepatitis C transmission; Iranduba; CIÊNCIAS BIOLÓGICAS: IMUNOLOGIA

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Andrade, M. B. P. d. (2013). Caracterização epidemiológica, virológica e imunológica de voluntários anti-HCV reativos do município de Iranduba, região metropolitana de Manaus. (Masters Thesis). Universidade Federal do Amazonas. Retrieved from http://tede.ufam.edu.br/handle/tede/2626

Chicago Manual of Style (16^th Edition):

Andrade, Midiã Barbosa Pimentel de. “Caracterização epidemiológica, virológica e imunológica de voluntários anti-HCV reativos do município de Iranduba, região metropolitana de Manaus.” 2013. Masters Thesis, Universidade Federal do Amazonas. Accessed January 24, 2021. http://tede.ufam.edu.br/handle/tede/2626.

MLA Handbook (7^th Edition):

Andrade, Midiã Barbosa Pimentel de. “Caracterização epidemiológica, virológica e imunológica de voluntários anti-HCV reativos do município de Iranduba, região metropolitana de Manaus.” 2013. Web. 24 Jan 2021.

Vancouver:

Andrade MBPd. Caracterização epidemiológica, virológica e imunológica de voluntários anti-HCV reativos do município de Iranduba, região metropolitana de Manaus. [Internet] [Masters thesis]. Universidade Federal do Amazonas; 2013. [cited 2021 Jan 24]. Available from: http://tede.ufam.edu.br/handle/tede/2626.

Council of Science Editors:

Andrade MBPd. Caracterização epidemiológica, virológica e imunológica de voluntários anti-HCV reativos do município de Iranduba, região metropolitana de Manaus. [Masters Thesis]. Universidade Federal do Amazonas; 2013. Available from: http://tede.ufam.edu.br/handle/tede/2626

22. Frakolaki, Efseveia. Αξιολόγηση νέων αναστολέων του ιού της Ηπατίτιδας C και συγγενικών ιών, καθώς και βιοδεικτών απόκρισης σε θεραπευτικές προσεγγίσεις.

Degree: 2019, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/46119

► Despite the effective treatments against Hepatitis C virus (HCV), problems still exist, such as high treatment costs, the emergence of resistant viral strains, and the… (more)

▼ Despite the effective treatments against Hepatitis C virus (HCV), problems still exist, such as high treatment costs, the emergence of resistant viral strains, and the co-infections with Hepatitis B Virus (HBV) and the related to HCV Dengue (DENV), Yellow Fever (YFV) and Zika (ZIKV) viruses. Moreover, the cellular mechanisms that regulate viral replication are not fully understood. Therefore, in this project a) new inhibitors against viruses of Flaviviridae family (HCV, DENV, YFV, ZIKV) and the molecular mechanisms of action were studied, and b) the role of cellular factors in viral replication and pathogenesis was investigated, to highlight new biomarkers and therapeutic targets against Flaviviridae viruses, by focusing on the signaling pathways of hypoxia, PI3K/AKT and a PI3K-binding factor, L-Dopa decarboxylase (DDC). Concerning the first objective, we tested against Flaviviridae viruses: 1) plant extracts and isolated compounds, 2) nucleoside and peptidomimetic synthetic analogues based on approved anti-HCV drugs, and 3) analogues of N-hydroxyimide and acetohydroxamic acid, which chelate divalent metal ions in the active site of viral enzymes. Specifically, we found compounds broadly active against HCV, DENV and YFV, while the flavonoid 2'-hydroxygensitein-7-O-glucopyranoside was effective against DENV (median effective concentration-EC50=6.38 μM). Nucleoside analogue 15d inhibited HCV 1b (EC50=40.22 μM), with high barrier to resistance. The resistance mutations suggest that it inhibits the interactions of NS5A. The N-hydroxyimide ZF66 had EC50=3.08 μM against HCV 1b, while exhibiting a high barrier to resistance, and possibly chelates the Mg2+ ions in the active site of NS5B polymerase. Finally, acetohydroxamic acid analogues bearing an adamantane group as a substituent, have broad activity against HCV, DENV, YFV and ZIKV), with ZB5 (HCV 1b: EC50=0.08 μM) and ZF64 (DENV: EC50=0.07 μM) being the most active. They also showed a high barrier to resistance and possibly target viral NS3 protein. Concerning the second objective, hypoxia (3% v/v O2) increased DENV RNA replication, through factors that activate under low oxygen, specifically HIF and AKT, but not PI3K. In addition, hypoxia reduced the activity of HCV and DENV inhibitors. Inversely, DENV induces HIF under atmospheric oxygen, causing a metabolic reprogramming in the host cell that favors viral proliferation. In addition, DENV and HCV infection negatively affect the expression of DDC, while DDC negatively affects viral replication. The negative correlation between HCV RNA and DDC mRNA was confirmed in liver biopsies from patients with chronic HCV infection. Overall, broadly effective inhibitors targeting Flaviviridae viruses were detected, which could be developed as antivirals, or against co-infections and emerging viruses. In addition, factors regulated by hypoxia and PI3K/AKT pathways were found to play an important role in the replication of Flaviviridae viruses, such as HIF (DENV) and DDC (HCV, DENV), which could be used as antiviral targets, or…

Subjects/Keywords: Ιός ηπατίτιδας C (HCV); Αντιϊκά; Ιός δάγκειου πυρετού (DENV); Υποξία; Hepatitis C virus (HCV); Antivirals; Dengue virus (DENV); Hypoxia

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Frakolaki, E. (2019). Αξιολόγηση νέων αναστολέων του ιού της Ηπατίτιδας C και συγγενικών ιών, καθώς και βιοδεικτών απόκρισης σε θεραπευτικές προσεγγίσεις. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/46119

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Frakolaki, Efseveia. “Αξιολόγηση νέων αναστολέων του ιού της Ηπατίτιδας C και συγγενικών ιών, καθώς και βιοδεικτών απόκρισης σε θεραπευτικές προσεγγίσεις.” 2019. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed January 24, 2021. http://hdl.handle.net/10442/hedi/46119.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Frakolaki, Efseveia. “Αξιολόγηση νέων αναστολέων του ιού της Ηπατίτιδας C και συγγενικών ιών, καθώς και βιοδεικτών απόκρισης σε θεραπευτικές προσεγγίσεις.” 2019. Web. 24 Jan 2021.

Vancouver:

Frakolaki E. Αξιολόγηση νέων αναστολέων του ιού της Ηπατίτιδας C και συγγενικών ιών, καθώς και βιοδεικτών απόκρισης σε θεραπευτικές προσεγγίσεις. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2019. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/10442/hedi/46119.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Frakolaki E. Αξιολόγηση νέων αναστολέων του ιού της Ηπατίτιδας C και συγγενικών ιών, καθώς και βιοδεικτών απόκρισης σε θεραπευτικές προσεγγίσεις. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2019. Available from: http://hdl.handle.net/10442/hedi/46119

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

23. Παυλίδης, Χρίστος. Διακυμάνσεις γκρελίνης και λεπτίνης σε ασθενείς με χρόνια ηπατίτιδα c και μη αλκοολική λιπώδη διήθηση ήπατος.

Degree: 2012, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/29514

► Hepatic steatosis is a common histological feature in patients with chronichepatitis C and can be attributed to a variety of mechanisms which areconsidered to play… (more)

▼ Hepatic steatosis is a common histological feature in patients with chronichepatitis C and can be attributed to a variety of mechanisms which areconsidered to play a role in the pathogenesis of non-alcoholic fatty liver disease,together with the direct effect of hepatitis virus C primarily in hepatic metabolismlipids.Leptin has an essential role in the regulation of body fat inducing insulinresistance and increasing the concentration of fatty acids in the liver resulting inincreased peroxidation and the development of steatosis. Also thought to act as amediator of the immune response through enhancing proinflammatory cytokinesand development of liver fibrosis. .Ghrelin is a peptide recently discovered and considered to be involved in energymetabolism, food intake and glucose homeostasis. .The hepatic steatosis is an independent factor of negative response to antiviraltherapy.Aims of this study were the association between serum levels of leptin andghrelin in patients with chronic hepatitis C and hepatic steatosis with otherclinical, biochemical, virological and histological parameters before and aftertreatment, the effect of steatosis and levels of leptin and ghrelin n theachievement of virologic response, but also the effect of antiviral therapy in leptinand ghrelin levels. .We analyzed 96 patients with confirmed serologically, virologically andhistologically chronic hepatitis C and hepatic steatosis. The 56 patients hadgenotype 1 and 40 had genotype 3. Patients with other causes of chronic liverdisease or decompensated liver cirrhosis were excluded from the study. Allpatients were evaluated clinically, biochemically and hematology at weeks 2, 4,8, 12, 24, 48 and 72 from the beginning of treatment. The histologicalclassification of biopsies were done for chronic hepatitis according to Ishak scoreand for the hepatic steatosis according to Brunt score. .Leptin levels before treatment were significantly higher and correlated with thedegree of steatosis in patients with genotype 1 and negatively correlated with theprobability of success in antiviral treatment. Ghrelin was higher in responderspatients of both genotypes. In patients with genotype 3, leptin levels did notcorrelate with the degree of steatosis while, responders ghrelin levels showed176significant reduction after treatment. In patients with genotype 3, the levels ofviral load at screening were correlated proportionally with the degree of steatosis.In conclusion, sustained virological response (SVR) in patients with genotype 1were positively correlated with a lower degree of steatosis and fibrosis and higherghrelin levels before treatment, while negatively correlated with high levels ofleptin before treatment. In patients with genotype 3 SVR was correlatednegatively with advanced fibrosis and increased insulin resistance but does notseem to have any correlation with the degree of steatosis or leptin levels beforetreatment. In the future, it is proposed to take into account the levels of leptin inthe achievement of sustained virological response in patients…

Subjects/Keywords: Ηπατίτιδα C, Ιός (HCV); Γκρελίνη; Λεπτίνη; Στεάτωση; Μόνιμη ιολογική ανταπόκριση; Hepatitis C, Virus (HCV); Ghrelin; Leptine; Steatosis; Sustained virological response

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Παυλίδης, . �. (2012). Διακυμάνσεις γκρελίνης και λεπτίνης σε ασθενείς με χρόνια ηπατίτιδα c και μη αλκοολική λιπώδη διήθηση ήπατος. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/29514

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Παυλίδης, Χρίστος. “Διακυμάνσεις γκρελίνης και λεπτίνης σε ασθενείς με χρόνια ηπατίτιδα c και μη αλκοολική λιπώδη διήθηση ήπατος.” 2012. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed January 24, 2021. http://hdl.handle.net/10442/hedi/29514.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Παυλίδης, Χρίστος. “Διακυμάνσεις γκρελίνης και λεπτίνης σε ασθενείς με χρόνια ηπατίτιδα c και μη αλκοολική λιπώδη διήθηση ήπατος.” 2012. Web. 24 Jan 2021.

Vancouver:

Παυλίδης �. Διακυμάνσεις γκρελίνης και λεπτίνης σε ασθενείς με χρόνια ηπατίτιδα c και μη αλκοολική λιπώδη διήθηση ήπατος. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2012. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/10442/hedi/29514.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Παυλίδης �. Διακυμάνσεις γκρελίνης και λεπτίνης σε ασθενείς με χρόνια ηπατίτιδα c και μη αλκοολική λιπώδη διήθηση ήπατος. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2012. Available from: http://hdl.handle.net/10442/hedi/29514

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Indian Institute of Science

24. Bose, Mihika. Characterisation of Monoclonal Antibodies and Small Molecule Inhibitors as Hepatitis C Virus Entry Inhibitors.

Degree: PhD, Faculty of Science, 2017, Indian Institute of Science

URL: http://etd.iisc.ac.in/handle/2005/2920

► Hepatitis C virus (HCV) represents a global health threat. HCV is a blood-borne positive-strand RNA virus belonging to the Flaviviridae family that infects ~160 million… (more)

▼ Hepatitis C virus (HCV) represents a global health threat. HCV is a blood-borne positive-strand RNA virus belonging to the Flaviviridae family that infects ~160 million people worldwide. About 70% of infected individuals fail to clear the virus and subsequently develop chronic hepatitis, frequently leading to liver cirrhosis and in some cases hepatocellular carcinoma. Therapeutic options for HCV infection are still limited and a protective vaccine is not yet available. Currently available therapies include administration of pegylated alpha interferon in combination with ribavirin. The recently approved protease inhibitors Boceprevir and Telaprevir are also included in the treatment regimen. However, limitations to the treatment with direct-acting antivirals (DAAs) are associated with severe side effects and low sustained virological response (SVR) rates that vary depending on the virus and host genotype. The replication step of the viral life cycle is mostly targeted by majority of DAAs. Recent findings have suggested that a combination of entry inhibitors together with DAAs exhibit a synergistic effect in the treatment of HCV. Therefore, identification of efficient HCV entry inhibitors is of high priority In vitro studies have shown that HCV attachment and subsequent entry into the host cells is mediated by E1 and E2 viral envelope proteins. HCV entry requires interaction with a number of receptors which include CD81, scavenger receptor B1 (SR-B1) and the tight junction proteins, claudin 1 (CLDN1) and occludin (OCLN). Since the E2 glycoprotein is reported to interact directly with cellular receptors, it is an attractive target for neutralisation. The present study focuses on the establishment and characterisation of entry inhibitors as antivirals for HCV. The thesis is presented in three chapters: Chapter 1- ‘Introduction’, provides a brief overview on HCV genotypes, genome organisation, life cycle including details on the entry process and therapies used for the treatment of HCV. Chapter 2 describes the generation of monoclonal antibodies (mAbs) against HCV envelope proteins as potent anti-viral agents for the prevention of HCV infection. Data on the identification and characterization of the neutralizing epitopes of HCV envelope proteins have been presented. Chapter 3 includes isolation of entry inhibitors of HCV from natural sources and identification and characterization of the active components exhibiting antiviral property. A number of studies have reported the role of neutralizing antibodies in the course of HCV infection and emerging data suggest protective effect of antibodies against HCV infection. Most of the ongoing studies are based on HCV genotype 1a which is prevalent globally. However in India, the prevalent genotype is 3a. Therefore, we established a panel of mAbs against HCV-LPs comprising of core-E1-E2 derived from genotype 3a as described in chapter 2. HCV-LP based system has been used in this study since it mimics the biophysical conformation, morphology and antigenic properties of the native… Advisors/Committee Members: Karande, Anjali A (advisor).

Subjects/Keywords: Hepatitis C Virus; Monoclonal Antibodies; Hepatitis C Virus Infection; Hepatitis C Virus Entry; HCV Small Molecule Inhibitors; Hepatitis C Virus Treatment; HCV Treatment; Rutin; Hepatitis C Virus E2 Protein; Biochemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bose, M. (2017). Characterisation of Monoclonal Antibodies and Small Molecule Inhibitors as Hepatitis C Virus Entry Inhibitors. (Doctoral Dissertation). Indian Institute of Science. Retrieved from http://etd.iisc.ac.in/handle/2005/2920

Chicago Manual of Style (16^th Edition):

Bose, Mihika. “Characterisation of Monoclonal Antibodies and Small Molecule Inhibitors as Hepatitis C Virus Entry Inhibitors.” 2017. Doctoral Dissertation, Indian Institute of Science. Accessed January 24, 2021. http://etd.iisc.ac.in/handle/2005/2920.

MLA Handbook (7^th Edition):

Bose, Mihika. “Characterisation of Monoclonal Antibodies and Small Molecule Inhibitors as Hepatitis C Virus Entry Inhibitors.” 2017. Web. 24 Jan 2021.

Vancouver:

Bose M. Characterisation of Monoclonal Antibodies and Small Molecule Inhibitors as Hepatitis C Virus Entry Inhibitors. [Internet] [Doctoral dissertation]. Indian Institute of Science; 2017. [cited 2021 Jan 24]. Available from: http://etd.iisc.ac.in/handle/2005/2920.

Council of Science Editors:

Bose M. Characterisation of Monoclonal Antibodies and Small Molecule Inhibitors as Hepatitis C Virus Entry Inhibitors. [Doctoral Dissertation]. Indian Institute of Science; 2017. Available from: http://etd.iisc.ac.in/handle/2005/2920

25. Simeon, Rudo Lyndon. Selecting a path against Hepatitis C Virus.

Degree: 2013, Texas Digital Library

URL: http://hdl.handle.net/1969; http://hdl.handle.net/2249.1/66834

► Hepatitis C virus (HCV) currently affects ~5% of the world???s population and has relatively limited treatment options for infected patients. Genetic suppressor elements (GSE) derived… (more)

▼ Hepatitis C virus (HCV) currently affects ~5% of the world???s population and has relatively limited treatment options for infected patients. Genetic suppressor elements (GSE) derived from a gene or genome of interest can act as transdominant inhibitors of a particular biology function presumably by binding to and blocking an essential interaction surface for protein activity. Taking advantage of hepatoma cell line n4mBid, that supports all stages of the HCV life cycle and strongly report HCV infection by a cell-death phenotype, we developed an iterative selection/enrichment strategy for the identification of GSE against HCV. Using this strategy, a library expressing random fragments of the HCV genome was screened for sequences able to suppress HCV infection. A 244 amino acid gene fragment, B1, was strongly enriched after 5 rounds of selection. B1 has a very high net positive charge of 43 at neutral pH and a high charge-to-mass (kDa) ratio of 1.5. We show that B1 expression specifically inhibits HCV replication, apparently due to its high positive charge. We also show that recombinant positively charged proteins can inhibit HCV infection, when supplied in vitro.In addition, eGFP-fused B1 potently penetrates both adherent and suspension cells with >80% of cells taking up the protein. Importantly, we show that B1 not only facilitates cellular uptake, but allows protein cargo to reach sites of biological relevance. B1 also delivers non-covalently conjugated RNA and DNA across the cell membrane to cytosolic and nuclear sites, with efficiency comparable to commercially available cationic lipid reagents. Our data suggest that B1 utilizes cell-surface glycans and multiple competing endocytic pathways to enter and traffic through cells.During a separate screening carried out in our lab, we identified a TACR3 inhibitor SB 222200 that had significant HCV activity. We go on to show that both TACR1 and TACR3 receptors are expressed in the HCV-permissive Huh 7.5 cell line. We also show that both TACR1 and TACR3 inhibitors significantly inhibit HCV infection. These results point to the potential for TACR1 antagonists in treating patients infected with both HCV and HIV. Advisors/Committee Members: Chen, Zhilei (advisor).

Subjects/Keywords: HCV

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Simeon, R. L. (2013). Selecting a path against Hepatitis C Virus. (Thesis). Texas Digital Library. Retrieved from http://hdl.handle.net/1969; http://hdl.handle.net/2249.1/66834

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Simeon, Rudo Lyndon. “Selecting a path against Hepatitis C Virus.” 2013. Thesis, Texas Digital Library. Accessed January 24, 2021. http://hdl.handle.net/1969; http://hdl.handle.net/2249.1/66834.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Simeon, Rudo Lyndon. “Selecting a path against Hepatitis C Virus.” 2013. Web. 24 Jan 2021.

Vancouver:

Simeon RL. Selecting a path against Hepatitis C Virus. [Internet] [Thesis]. Texas Digital Library; 2013. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/1969; http://hdl.handle.net/2249.1/66834.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Simeon RL. Selecting a path against Hepatitis C Virus. [Thesis]. Texas Digital Library; 2013. Available from: http://hdl.handle.net/1969; http://hdl.handle.net/2249.1/66834

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

26. Burke, Kelly. Interrogating the Human CD8+ T Cell Response in HCV Infection.

Degree: 2014, Johns Hopkins University

URL: http://jhir.library.jhu.edu/handle/1774.2/36946

► Hepatitis C virus (HCV) infects nearly 170 million individuals worldwide and remains one of the leading causes of cirrhosis and hepatocellular carcinoma. Approximately 15-20% of… (more)

▼ Hepatitis C virus (HCV) infects nearly 170 million individuals worldwide and remains one of the leading causes of cirrhosis and hepatocellular carcinoma. Approximately 15-20% of individuals will spontaneously clear the infection, with the CD8+ T cell response crucial in achieving control. Given the highly diverse nature of HCV, the T cell response elicited by vaccines against HCV must achieve maximum cross reactivity against widely divergent circulating strains. We assessed T cell recognition of potential hepatitis C virus vaccine sequences generated using three rational approaches: 1) combining epitopes with predicted tight binding to the major histocompatibility complex (MHC), 2) consensus sequence (most common amino acid at each position), and 3) representative ancestral sequence that had been derived using Bayesian phylogenetic tools. No correlation was seen between peptide MHC binding affinity and frequency of recognition as measured by an interferon-gamma T cell response in human leukocyte antigen-matched HCV infected individuals. CD8+ T cells expanded with representative sequence HCV generally more broadly and robustly recognized highly diverse circulating HCV strains than T cell expanded with either consensus sequence or naturally occurring sequence variants. These data support the use of representative sequence in HCV vaccine design. In addition, we hypothesized that ongoing recognition of antigen with continued T cell receptor signaling is associated with upregulation of inhibitory T cell receptors. We characterized evolution of the CD8+ T cell response in the setting of absent, partial or incomplete, and complete recognition of antigen over time to determine if there is an association between prolonged antigenic stimulation and expression of inhibitory receptors. HCV-specific CD8+ T cell from HCV-infected subjects in a prospective longitudinal cohort were phenotyped for the activation markers HLA-DR and CD38; co-inhibitory receptors PD-1, TIM-3 and 2B4; and the memory molecule CD127 using polychromatic flow cytometry. The phenotype of these cells was compared in the setting of intact, mutated, and absent viral antigen within and across subjects. Our data point to the role of ongoing recognition of intact antigen in the T cell response to chronic viral infection Advisors/Committee Members: Siliciano, Robert F (advisor).

Subjects/Keywords: HCV; hepatitis C virus; CD8 T cell; immunology; vaccine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Burke, K. (2014). Interrogating the Human CD8+ T Cell Response in HCV Infection. (Thesis). Johns Hopkins University. Retrieved from http://jhir.library.jhu.edu/handle/1774.2/36946

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Burke, Kelly. “Interrogating the Human CD8+ T Cell Response in HCV Infection.” 2014. Thesis, Johns Hopkins University. Accessed January 24, 2021. http://jhir.library.jhu.edu/handle/1774.2/36946.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Burke, Kelly. “Interrogating the Human CD8+ T Cell Response in HCV Infection.” 2014. Web. 24 Jan 2021.

Vancouver:

Burke K. Interrogating the Human CD8+ T Cell Response in HCV Infection. [Internet] [Thesis]. Johns Hopkins University; 2014. [cited 2021 Jan 24]. Available from: http://jhir.library.jhu.edu/handle/1774.2/36946.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Burke K. Interrogating the Human CD8+ T Cell Response in HCV Infection. [Thesis]. Johns Hopkins University; 2014. Available from: http://jhir.library.jhu.edu/handle/1774.2/36946

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Penn State University

27. Cordek, Daniel Girard. Regulation of hepatitis C virus NS5A protein function by host-mediated phosphorylation.

Degree: 2012, Penn State University

URL: https://submit-etda.libraries.psu.edu/catalog/15982

► We have established a paradigm for studying host-mediated phosphorylation of hepatitis C virus (HCV) nonstructural protein 5A (NS5A), from mapping phosphorylated residues on the viral… (more)

▼ We have established a paradigm for studying host-mediated phosphorylation of hepatitis C virus (HCV) nonstructural protein 5A (NS5A), from mapping phosphorylated residues on the viral protein, to biochemical analysis of these modifications in vitro, to verification of biological significance via cell culture- and human liver-based assays. Using this approach, we have determined protein kinase A (PKA)-mediated phosphorylation of NS5A impacts accumulation of HCV RNA and other viral proteins. Through the use of immunological reagents designed to specifically detect PKA-phosphorylated NS5A, we have shown this modified form of the protein is a subset of all NS5A present in cells stably replicating HCV RNA. Further, as the amount of PKA-phosphorylated NS5A correlated to the severity of liver fibrosis in biopsy samples, this may be a novel, noninvasive biomarker for the staging of HCV-induced liver disease. Our analysis of NS5A phosphorylation by casein kinase 1α (CK1α) has identified the modification of a previously unidentified serine residue as critical to the formation of the hyperphosphorylated form of NS5A in vitro, the first time this elusive species of the viral protein has ever been systematically and reproducibly generated solely with NS5A and CK1α. Loss of this phosphorylation via phosphoablative substitutions in the HCV subgenomic replicon permitted persistent replication in the absence of previously identified cell culture adaptive mutations. Finally, we provide biochemical analysis suggesting that domain I of the viral protein may serve as a kinase recruitment domain. Truncations of NS5A lacking domain I were phosphorylated less efficiently than full-length forms of NS5A, despite the absence of phospho-residues in this region of the viral protein. RNA-induced homodimerization of domain I further inhibited phosphorylation of full-length NS5A, perhaps by blocking residues involved in kinase recruitment. Advisors/Committee Members: Craig Eugene Cameron, Dissertation Advisor/Co-Advisor, Craig Eugene Cameron, Committee Chair/Co-Chair, Paul Lee Babitzke, Committee Member, Philip C. Bevilacqua, Committee Member, Andrey S Krasilnikov, Committee Member, Lorraine C Santy, Committee Member.

Subjects/Keywords: hepatitis C virus; HCV; NS5A; phosphorylation; intrinsic disorder; IDP; RNA-binding

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cordek, D. G. (2012). Regulation of hepatitis C virus NS5A protein function by host-mediated phosphorylation. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/15982

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cordek, Daniel Girard. “Regulation of hepatitis C virus NS5A protein function by host-mediated phosphorylation.” 2012. Thesis, Penn State University. Accessed January 24, 2021. https://submit-etda.libraries.psu.edu/catalog/15982.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cordek, Daniel Girard. “Regulation of hepatitis C virus NS5A protein function by host-mediated phosphorylation.” 2012. Web. 24 Jan 2021.

Vancouver:

Cordek DG. Regulation of hepatitis C virus NS5A protein function by host-mediated phosphorylation. [Internet] [Thesis]. Penn State University; 2012. [cited 2021 Jan 24]. Available from: https://submit-etda.libraries.psu.edu/catalog/15982.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cordek DG. Regulation of hepatitis C virus NS5A protein function by host-mediated phosphorylation. [Thesis]. Penn State University; 2012. Available from: https://submit-etda.libraries.psu.edu/catalog/15982

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Addis Ababa University

28. Jijiga, Edosa. Assessment of Transfusion Transmissible Infections Among Blood Donors (A six years study) and Strategy on Direct Laboratory Testing Cost of Blood Screening at National Blood Transfusion Service of Addis Ababa, Ethiopia.

Degree: 2014, Addis Ababa University

URL: http://etd.aau.edu.et/dspace/handle/123456789/5846

► Abstract Background: Hepatitis B virus (HBV), hepatitis C virus (HCV), human immunodeficiency virus (HIV) and syphilis are the most serious infections transmitted during blood transfusion.… (more)

▼ Abstract Background: Hepatitis B virus (HBV), hepatitis C virus (HCV), human immunodeficiency virus (HIV) and syphilis are the most serious infections transmitted during blood transfusion. In such a resource limited setting, cheaper and feasible alternative strategies for blood donations testing are speciﬁcally required. However, updated data on the transfusion transmissible infections (TTIs), and cost effective strategies of blood screening are lacking. Objective: To determine the prevalence of transfusion transmissible infections among blood donorsfrom July 2008 to July 2013and propose cost effective strategy of blood screening at National Blood Bank of Addis Ababa, Ethiopia. Methodology:A retrospective analysis of blood donors’ record covering the period from July 2008 to July 2013 was conducted. The data was collected from the National Blood Transfusion Services (NBTS) of Addis Ababa and includes category of all donors and result for TTI markers. In addition, direct laboratory costs of parallel versus sequential strategy of blood screening were compared. To compare the strategies we used the current price of the laboratory costs. Data was first exported to Excel spread sheet from the institution’s data base and then finally exported to SPSS version 16 software (SPSS INC, Chicago, IL, USA) for analysis.Data analysis was performed using scores and odds ratio using same software to look for an association between dependent and independent variables. P values less than 0.05 were considered significant. Result: A total of 173,207 consecutive blood donors were screened between 2008 and 2013.The overall seroprevalence rate ofHBV, HIV, HCV and syphilis of blood donors was 5.0%, 1.6%, 1.4% and 0.1%respectively. The HIV-HBV confection was higher among blood donors 135(41.79%) followed by HBV-HCV co-infection which accounts about 103(31.89%). Significantly increased seroprevalence of TTI’s was observed in the age groups of 17-25 and 2635 years. In this study, the difference in cost between the current in use strategy (Parallel) versus our proposed newly designed sequential testing algorithm was 746,773.90 ETB. Conclusion: A significant percentage of the blood donors harbor TTIs. Higher prevalence of TTIs was observed among youths and replacement donors. The direct laboratory cost analysis using current in use strategy (parallel) was higher than the newly designed sequential testing algorithm. Thus, the new strategy can be implemented to make screening of TTIs cost effective in the face of the current effort of large mobilization of voluntary blood donors in the country. Advisors/Committee Members: Aster Tsegaye(MSc, PhD) (advisor).

Subjects/Keywords: Hepatitis B virus (HBV); hepatitis C virus (HCV); (HIV); syphilis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jijiga, E. (2014). Assessment of Transfusion Transmissible Infections Among Blood Donors (A six years study) and Strategy on Direct Laboratory Testing Cost of Blood Screening at National Blood Transfusion Service of Addis Ababa, Ethiopia. (Thesis). Addis Ababa University. Retrieved from http://etd.aau.edu.et/dspace/handle/123456789/5846

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Jijiga, Edosa. “Assessment of Transfusion Transmissible Infections Among Blood Donors (A six years study) and Strategy on Direct Laboratory Testing Cost of Blood Screening at National Blood Transfusion Service of Addis Ababa, Ethiopia. ” 2014. Thesis, Addis Ababa University. Accessed January 24, 2021. http://etd.aau.edu.et/dspace/handle/123456789/5846.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Jijiga, Edosa. “Assessment of Transfusion Transmissible Infections Among Blood Donors (A six years study) and Strategy on Direct Laboratory Testing Cost of Blood Screening at National Blood Transfusion Service of Addis Ababa, Ethiopia. ” 2014. Web. 24 Jan 2021.

Vancouver:

Jijiga E. Assessment of Transfusion Transmissible Infections Among Blood Donors (A six years study) and Strategy on Direct Laboratory Testing Cost of Blood Screening at National Blood Transfusion Service of Addis Ababa, Ethiopia. [Internet] [Thesis]. Addis Ababa University; 2014. [cited 2021 Jan 24]. Available from: http://etd.aau.edu.et/dspace/handle/123456789/5846.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jijiga E. Assessment of Transfusion Transmissible Infections Among Blood Donors (A six years study) and Strategy on Direct Laboratory Testing Cost of Blood Screening at National Blood Transfusion Service of Addis Ababa, Ethiopia. [Thesis]. Addis Ababa University; 2014. Available from: http://etd.aau.edu.et/dspace/handle/123456789/5846

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Addis Ababa University

29. Yacob, Mohammed. Prevalence of Hepatitis B, Hepatitis C and HIV among Chronic liver disease patients in selected hospitals, Addis Ababa, Ethiopia .

Degree: 2014, Addis Ababa University

URL: http://etd.aau.edu.et/dspace/handle/123456789/5853

► Abstract Introduction; Hepatitis B virus (HBV) and hepatitis C (HCV) virus is hepatotropic virus spread mainly through contaminated blood and blood products, sexual contact and… (more)

▼ Abstract Introduction; Hepatitis B virus (HBV) and hepatitis C (HCV) virus is hepatotropic virus spread mainly through contaminated blood and blood products, sexual contact and contaminated needles. Chronic infection by these viruses leads to slow progressive liver disease that over a period of up to 30 years may result in cirrhosis, chronic liver failure and hepatocellular carcinoma (HCC). HIV, HBV, and HCV infection share similar transmission routes and therefore co-infection is common. These viruses are prevalent in different parts of the world including Ethiopia there for this study shows the burden of these viruses in Ethiopia. Objective: To determine the prevalence of HBV, HCV and HIV infection among clinically diagnosed chronic liver disease patients visited at Black Lion, St. Paul and Armed force hospital Addis Ababa, Ethiopia. Method: Hospital based cross-sectional study was conducted in three selected hospitals of Addis Ababa over a period of 3 months (March2014- May 2014) on clinically diagnosed chronic liver disease patients. By using questionnaire brief history and risk factors was taken from each volunteering patient. Serum samples from each volunteering patients was screened for the presence of HBsAg and anti-HCV by ELISIA test kit and HIV by national test algorism. Result: A total of 117 participants who have chronic liver disease participated in the study, where 82 of them were males and the remaining 35 were females. The age distribution range form 18-78 years and the median age was 39 years. The overall prevalence of HBsAg, HCV and HIV was 34.2%, 18.8% and 9.4 respectively. The study participants had combined HBV/HIV, HCV/HIV and HBV/HCV infection which is possible because of their common modes of transmission. History of multiple sexual partner and blood transfusion also found statically significance with HBV and HCV virus infection respectively. Conclusion: The prevalence of HBV and HCV is high where as HIV is moderate among chronic liver disease patients and history of blood transfusion and multiple sexual partners was statistically associated with HCV and HBV infection respectively. Advisors/Committee Members: Ibrahim Ali, (BSc, MSC, PhD) (advisor).

Subjects/Keywords: Hepatitis B virus (HBV); hepatitis C (HCV); hepatotropic virus

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yacob, M. (2014). Prevalence of Hepatitis B, Hepatitis C and HIV among Chronic liver disease patients in selected hospitals, Addis Ababa, Ethiopia . (Thesis). Addis Ababa University. Retrieved from http://etd.aau.edu.et/dspace/handle/123456789/5853

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yacob, Mohammed. “Prevalence of Hepatitis B, Hepatitis C and HIV among Chronic liver disease patients in selected hospitals, Addis Ababa, Ethiopia .” 2014. Thesis, Addis Ababa University. Accessed January 24, 2021. http://etd.aau.edu.et/dspace/handle/123456789/5853.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yacob, Mohammed. “Prevalence of Hepatitis B, Hepatitis C and HIV among Chronic liver disease patients in selected hospitals, Addis Ababa, Ethiopia .” 2014. Web. 24 Jan 2021.

Vancouver:

Yacob M. Prevalence of Hepatitis B, Hepatitis C and HIV among Chronic liver disease patients in selected hospitals, Addis Ababa, Ethiopia . [Internet] [Thesis]. Addis Ababa University; 2014. [cited 2021 Jan 24]. Available from: http://etd.aau.edu.et/dspace/handle/123456789/5853.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yacob M. Prevalence of Hepatitis B, Hepatitis C and HIV among Chronic liver disease patients in selected hospitals, Addis Ababa, Ethiopia . [Thesis]. Addis Ababa University; 2014. Available from: http://etd.aau.edu.et/dspace/handle/123456789/5853

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of the Western Cape

30. Choi, Yook-Wah. Structural and functional characterization of human DDX5 and its interaction with NS5B of hepatitis C virus .

Degree: 2011, University of the Western Cape

URL: http://hdl.handle.net/11394/5299

► Hepatitis C was first recognized as a transfusion-associated liver disease not caused by hepatitis A or hepatitis B virus after serological tests were developed to… (more)

▼ Hepatitis C was first recognized as a transfusion-associated liver disease not caused by hepatitis A or hepatitis B virus after serological tests were developed to screen for their presence in the blood. The infectious agent was finally identified with the cloning of the cDNA of hepatitis C virus (HCV) using random polymerase chain reaction (PCR) screening of nucleic acids extracted from plasma of a large pool of chimpanzee infected with non-A non-B hepatitis. NS5B, a membrane-associated RNA-dependent RNA polymerase essential in the replication of HCV, initiates the synthesis of a complementary negative-strand RNA from the genomic positive-strand RNA so that more positive-strand HCV RNA can then be generated from the newly synthesised negative-strand template. The crystal structure of NS5B presented typical fingers, palm and thumb sub-domains encircling the GDD active site, which is also seen in other RNA-dependent RNA polymerases, and is similar to the structure of reverse transcriptase of HIV-1 and murine Moloney leukaemia virus. The last 21 amino acids in the C-terminus of NS5B anchor the protein to the endoplasmic reticulum (ER)-derived membranous web. NS5B has been shown to interact with the core, NS3/NS4A, NS4B and NS5A proteins, either directly or indirectly. Numerous interactions with cellular proteins have also been reported. These proteins are mainly associated with genome replication, vesicular transport, protein kinase C-related kinase 2, P68 (DDX5), α-actinin, nucleolin, human eukaryotic initiation factor 4AII, and human VAMP-associated protein. Previous studies have confirmed that NS5B binds to full-length DDX5. By constructing deletion mutants of DDX5, we proceeded to characterize this interaction between DDX5 and HCV NS5B. We report here the identification of two exclusive HCV NS5B binding sites in DDX5, one in the N-terminal region of amino acids 1 to 384 and the other in the C-terminal region of amino acids 387 to 614. Proteins spanning different regions of DDX5 were expressed and purified for crystallization trials. The N-terminal region of DDX5 from amino acids 1 to 305 which contains the conserved domain I of the DEAD-box helicase was also cloned and expressed in Escherichia coli. The cloning, expression, purification and crystallization conditions are presented in this work. Subsequently, the crystal structure of DDX5 1-305 was solved and the high resolution three-dimensional structure shows that in front of domain I is the highly variable and disordered N terminal region (NTR) of which amino acids 51-78 is observable, but whose function is unknown. This region forms an extensive loop and supplements the core with an additional α-helix. Co-immunoprecipitation experiments demonstrated that the NTR of DDX5 1-305 auto-inhibit its interaction with NS5B. Interestingly, the α-helix in NTR is essential for this auto-inhibition and seems to mediate the interaction between the highly flexible 1-60 residues in NTR and NS5B binding site in DDX5 1-305, presumably located within residues 79-305.… Advisors/Committee Members: C%22%29&pagesize-30">Fielding, B.C (advisor), Tan, YJ (advisor).

Subjects/Keywords: Hepatitis C virus (HCV); GST fusion protein; NS5B; Protein crystallisation screens

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Choi, Y. (2011). Structural and functional characterization of human DDX5 and its interaction with NS5B of hepatitis C virus . (Thesis). University of the Western Cape. Retrieved from http://hdl.handle.net/11394/5299

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Choi, Yook-Wah. “Structural and functional characterization of human DDX5 and its interaction with NS5B of hepatitis C virus .” 2011. Thesis, University of the Western Cape. Accessed January 24, 2021. http://hdl.handle.net/11394/5299.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Choi, Yook-Wah. “Structural and functional characterization of human DDX5 and its interaction with NS5B of hepatitis C virus .” 2011. Web. 24 Jan 2021.

Vancouver:

Choi Y. Structural and functional characterization of human DDX5 and its interaction with NS5B of hepatitis C virus . [Internet] [Thesis]. University of the Western Cape; 2011. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/11394/5299.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Choi Y. Structural and functional characterization of human DDX5 and its interaction with NS5B of hepatitis C virus . [Thesis]. University of the Western Cape; 2011. Available from: http://hdl.handle.net/11394/5299

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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Open Access Theses and Dissertations