publisher:("Università degli Studi di Milano")

1. G.M.G. Quaroni. TUNING THE MECHANICAL AND ADHESIVE PROPERTIES OF TRANS-DERMAL DRUG DELIVERY SYSTEMS.

Degree: 2018, Università degli Studi di Milano

► Drugs are usually applied onto the skin either for the treatment of localized diseases or systemic pathologies. Among the available dosage forms transdermal patches and… (more)

▼ Drugs are usually applied onto the skin either for the treatment of localized diseases or systemic pathologies. Among the available dosage forms transdermal patches and the newest film-forming systems are considered the most attractive dosage forms. Firmly adhering to the skin, they assure a prolonged release of the drug predetermining the administered dose and the area of application. Film-forming systems are composed by a polymeric solution or suspension which contains the active substance(s). Patches in their simplest configuration consist of a flexible backing layer, an adhesive matrix in which the active substance(s) are dissolved or dispersed and a removable release liner. Both such dosage forms must comply peculiar technological features. Film-forming systems should quickly dry on the skin and the formed film should be almost invisible and non-sticky. Furthermore, their mechanical properties should overcome the stresses caused by body movements. Transdermal patches have to be sticky and exhibit an optimal balance between adhesion and cohesion. Indeed, a patch should strongly adhere to the skin and be easily removed with no or little trauma. Moreover, a certain cohesiveness is necessary so that the patch will not move when applied in vivo and can be peeled away without living any residues. To satisfy the requirements of flexibility necessary to assure a proper skin/dosage form contact, a material should have an elastic modulus lower than that of the skin and, in the case of a patch, a viscoelastic pattern. Despite many products are currently available on the market, some critical issues, such as adhesive and mechanical properties, which affect the residence time of the dosage form onto the skin and subsequently its therapeutic efficacy, are still scarcely investigated. Hence, there is the need to study the overall relevant issues involved in designing film-forming systems and transdermal patches and clarify the possible relationships between their features. This kind of relationship could provide useful information to design and optimize both such dosage forms. This doctoral thesis aimed to evaluate the effect of the formulation compositions on rheological, adhesive and mechanical properties as well as on the in vitro biopharmaceutical performances of different polymeric matrices in order to design film forming systems and transdermal patches. In particular, the experimental work was focused on: (i) the design of film-forming systems based on an ammonium methacrylate copolymer (Eudragit RL) solubilized in a mixture of solvents in different ratios and plasticized by different amounts of plasticizer; the effects of solvent systems as well as the addition of the plasticizer were evaluated on drying time, outward stickiness, mechanical properties and in vitro biopharmaceutical performances; (ii) the design of transdermal patches based on differently plasticized ammonium methacrylate copolymer (Eudragit RL) and the evaluation of their rheological and tack properties, primarily to better understand… Advisors/Committee Members: tutor: P. Minghetti, coordinatore: G. Aldini, MINGHETTI, PAOLA, ALDINI, GIANCARLO.

Subjects/Keywords: Settore CHIM/09 - Farmaceutico Tecnologico Applicativo

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APA (6^th Edition):

Quaroni, G. (2018). TUNING THE MECHANICAL AND ADHESIVE PROPERTIES OF TRANS-DERMAL DRUG DELIVERY SYSTEMS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/540072

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Quaroni, G.M.G.. “TUNING THE MECHANICAL AND ADHESIVE PROPERTIES OF TRANS-DERMAL DRUG DELIVERY SYSTEMS.” 2018. Thesis, Università degli Studi di Milano. Accessed February 27, 2021. http://hdl.handle.net/2434/540072.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Quaroni, G.M.G.. “TUNING THE MECHANICAL AND ADHESIVE PROPERTIES OF TRANS-DERMAL DRUG DELIVERY SYSTEMS.” 2018. Web. 27 Feb 2021.

Vancouver:

Quaroni G. TUNING THE MECHANICAL AND ADHESIVE PROPERTIES OF TRANS-DERMAL DRUG DELIVERY SYSTEMS. [Internet] [Thesis]. Università degli Studi di Milano; 2018. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2434/540072.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Quaroni G. TUNING THE MECHANICAL AND ADHESIVE PROPERTIES OF TRANS-DERMAL DRUG DELIVERY SYSTEMS. [Thesis]. Università degli Studi di Milano; 2018. Available from: http://hdl.handle.net/2434/540072

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. C. Galli. NONCOVALENT FLUOROUS INTERACTIONS: NEW APPROACHES FOR DRUG DISCOVERY AND DRUG DELIVERY.

Degree: 2018, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/540156

► The unique chemical properties of fluorine atom (high electronegativity, high ionization potential, low polarizability and low van der Waals interactions) modify the chemical properties of… (more)

▼ The unique chemical properties of fluorine atom (high electronegativity, high ionization potential, low polarizability and low van der Waals interactions) modify the chemical properties of organic compounds as well as their reactivity when hydrogen atoms are replaced by fluorines. Actually, fluorocarbons show low polarity, which is responsible for the high hydrophobicity of these molecules. Additionally, the low polarizability of fluorines leads to weaker van der Waals interactions, which makes fluorocarbons lipophobic. Therefore, fluorinated compounds show an amphiphilic character that leads to the formation of the fluorous phase, which is separated to both aqueous and organic layers. The aim of my project was applying the strong and noncovalent fluorous interactions to drug discovery and drug delivery. The first part of my thesis is focused on the development of a new strategy for target identification able to overcome the several limitations associated to classic chemical proteomics techniques. Indeed, traditional chemical proteomics methodology uses agarose beads covalently bound to streptavidin as stationary phase for affinity purification. This resin is able to retain biotin-tagged proteins as well as sticky components abundant in the lysate. These contaminants might be aspecifically eluted with the biological targets, complicating the mass analysis and therefore the target identification. In order to increase the selectivity of the proteomics approach, we designed an innovative fluorous proteomics methodology using the strong fluorous-fluorous interactions as recognition system for affinity purification. Indeed, perfluorinated stationary phase can anchor only fluorinated species, avoiding aspecific binding. To test the fluorous proteomics approach, papain was considered as biological target. Fluorinated inhibitors of papain with different fluorinated-chain length were synthesized. The number of fluorine atom of the inhibitor is crucial for the interaction with the fluorinated stationary phase in the purification step. Actually, only papain inhibitors with a long fluorous alkyl chain are able to bind the fluorinated resin and therefore immobilize papain. In contrast, inhibitors with a short fluorous alkyl chain cannot bind the fluorinated stationary phase by means of fluorous-fluorous interactions. Consequently, papain cannot be anchored to the resin. The second part of my thesis is focused on the application of fluorous interactions for drug delivery. This project was carried out in the School of Pharmacy, University of Wisconsin-Madison (Madison, WI, U.S.A.) under the supervision of Professor Sandro Mecozzi. The aim was designing and synthesizing semifluorinated dibranched polymers. The synthesis of fluorinated molecules is a challenge, due to their poor reactivity and low solubility in commonly-used organic solvents. To increase the final yield, each step of the synthesis of the semifluorinated dibranched polymers was optimized. The dibranched fluorinated polymers will be used to prepare oil-in-water… Advisors/Committee Members: tutor: S. Romeo, coordinatore: G. Aldini, ROMEO, SERGIO, ALDINI, GIANCARLO.

Subjects/Keywords: Settore CHIM/08 - Chimica Farmaceutica

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Galli, C. (2018). NONCOVALENT FLUOROUS INTERACTIONS: NEW APPROACHES FOR DRUG DISCOVERY AND DRUG DELIVERY. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/540156

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Galli, C.. “NONCOVALENT FLUOROUS INTERACTIONS: NEW APPROACHES FOR DRUG DISCOVERY AND DRUG DELIVERY.” 2018. Thesis, Università degli Studi di Milano. Accessed February 27, 2021. http://hdl.handle.net/2434/540156.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Galli, C.. “NONCOVALENT FLUOROUS INTERACTIONS: NEW APPROACHES FOR DRUG DISCOVERY AND DRUG DELIVERY.” 2018. Web. 27 Feb 2021.

Vancouver:

Galli C. NONCOVALENT FLUOROUS INTERACTIONS: NEW APPROACHES FOR DRUG DISCOVERY AND DRUG DELIVERY. [Internet] [Thesis]. Università degli Studi di Milano; 2018. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2434/540156.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Galli C. NONCOVALENT FLUOROUS INTERACTIONS: NEW APPROACHES FOR DRUG DISCOVERY AND DRUG DELIVERY. [Thesis]. Università degli Studi di Milano; 2018. Available from: http://hdl.handle.net/2434/540156

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

3. G. Resmini. THE ROLE OF THE HYPOXIA INDUCIBLE FACTOR (HIF-1Α) IN MYOGENESIS.

Degree: 2018, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/541720

► Regeneration of skeletal muscle is a complex process that requires the activation of quiescent adult stem cells, the satellite cells, which are resident in hypoxic… (more)

▼ Regeneration of skeletal muscle is a complex process that requires the activation of quiescent adult stem cells, the satellite cells, which are resident in hypoxic niches in the tissue. This process is mainly regulated through a group of transcription factors known as the hypoxia-inducible factors (HIFs). In particular, HIF-1α activation has been described as beneficial for the cell to overcome an hypoxic insult, while it has been observed that its chronic activation completely inhibits skeletal muscle differentiation. Therefore, oxygen deprivation and HIF-1α may play a role in activating the initial steps of the regeneration process. Herein, we investigated whether a 24h pre-conditioning under physical (1% O2) or chemical (IOX2 and FG-4592, two commercial PHDs inhibitors) hypoxic culture conditions could alter the differentiation of C2C12 myoblasts. In this thesis work we report that a controlled stimulus can trigger HIF-1α, activating MyoD through the non-canonical Wnt/β-catenin pathway and resulting in muscle hypertrophy. In particular, results show that both an hypoxic and a chemical pre-conditioning promotes the increase of all differentiation markers and the up-regulation of the non-canonical WNT pathway involved in myogenesis. Moreover, HIF-1α silencing significantly reduced cell differentiation, down-regulating MyoD and MHC as well as the expression of WNT7a. Finally, we studied the mechanism of WNT7a activation mediated by HIF-1α. Our results showed that HIF-1α activation induced an enhancement of WNT7a promoter activity, therefore we focused on the identification of the HIF-1α binding sequences localized on WNT7a promoter. Two different regions of over-lapping were identified by ChIP experiments, validating that HIF-1α directly binds WNT7a promoter and regulates its gene expression. In conclusion, we demonstrated the crucial role played by HIF-1α during skeletal muscle differentiation, and our results revealed that PHDs inhibitors could be used to mimic the effects obtained under hypoxic conditions. In addition, we define HIF-1α as a new possible candidate to induce the activation of WNT7a, which characterized the hypertrophic phenotype of the skeletal muscle. Altogether these results support the notion that HIF-1α plays a pivotal role in activating the regeneration process and may suggest new perspective for novel therapeutic targets in the treatment of several muscle diseases. Advisors/Committee Members: supervisore: L. Anastasia, coordinatore: S. Sonnino, SONNINO, SANDRO.

Subjects/Keywords: Hypoxic pre-conditioning; Skeletal muscle differentiation; Myoblasts; Hypertrophy; PHDs inhibitors; HIF-1α; WNT7a; Settore BIO/10 - Biochimica

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Resmini, G. (2018). THE ROLE OF THE HYPOXIA INDUCIBLE FACTOR (HIF-1Α) IN MYOGENESIS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/541720

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Resmini, G.. “THE ROLE OF THE HYPOXIA INDUCIBLE FACTOR (HIF-1Α) IN MYOGENESIS.” 2018. Thesis, Università degli Studi di Milano. Accessed February 27, 2021. http://hdl.handle.net/2434/541720.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Resmini, G.. “THE ROLE OF THE HYPOXIA INDUCIBLE FACTOR (HIF-1Α) IN MYOGENESIS.” 2018. Web. 27 Feb 2021.

Vancouver:

Resmini G. THE ROLE OF THE HYPOXIA INDUCIBLE FACTOR (HIF-1Α) IN MYOGENESIS. [Internet] [Thesis]. Università degli Studi di Milano; 2018. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2434/541720.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Resmini G. THE ROLE OF THE HYPOXIA INDUCIBLE FACTOR (HIF-1Α) IN MYOGENESIS. [Thesis]. Università degli Studi di Milano; 2018. Available from: http://hdl.handle.net/2434/541720

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

4. G. MONZIO COMPAGNONI. ASSESSING THE PATHOGENESIS OF MULTIPLE SYSTEM ATROPHY THROUGH CELLULAR MODELS.

Degree: 2018, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/540359

► Multiple System Atrophy (MSA) is a severe adult-onset neurodegenerative disease, with an incidence of 0.6 per 100000 persons/year and a survival rate of 6-9 years… (more)

▼ Multiple System Atrophy (MSA) is a severe adult-onset neurodegenerative disease, with an incidence of 0.6 per 100000 persons/year and a survival rate of 6-9 years from the onset. MSA is clinically characterized by parkinsonism, cerebellar ataxia, dysautonomia and pyramidal signs. Two subtypes of the disease can be distinguished, MSA-P and MSA-C, on the basis of the predominant symptomatology, parkinsonian or cerebellar respectively. From a neuropathological point of view, MSA is classified as an alpha-synucleinopathy. However, differently from Parkinson’s disease and Dementia with Lewy bodies, the main intracellular localization of alpha-synuclein aggregates in MSA is not the neuron, but the oligodendrocyte. An effective therapy is not available yet. The pathogenesis of the disease is almost completely unknown and the available models, mainly transgenic animals overexpressing human alpha-synuclein under the promoter of genes specifically expressed in oligodendrocytes, do not fully recapitulate the pathogenic mechanisms. The aim of the present project was the generation and study of new experimental models for the identification of the molecular mechanisms of the disease and new therapeutic targets. The study has taken advantage from the use of peripheral blood cells, fibroblasts’ primary cultures, iPSC-derived dopaminergic neurons and brain tissue and has focused on several topics, including autophagy, mitochondrial functioning and alpha-synuclein expression. The main findings in patients have been: an increase of mtDNA amount in peripheral blood cells and in brain tissue, selectively in the cerebellar subtype; an impairment of mitochondrial respiratory chain activity in fibroblasts and iPSC-derived neurons; an increase of mitochondrial mass and of complex II amount, an increased expression of mitochondria-related genes, an increase of the amount of many enzymes involved in the synthesis of Coenzyme Q10 in neurons; an impairment of the autophagic pathway in neurons; a reduced expression of neuronal markers in advanced stages of dopaminergic differentiation; hypomethylation of a CpG island located in alpha-synuclein intron 1. The generation and characterization of the iPSC-derived neuronal model is particularly remarkable because it represents the first comprehensive model of MSA based on this technique. In conclusion, this project has led to the identification of several defects in MSA cells and tissues, suggesting the impairment of specific molecular pathways and contributing to the understanding of the underlying pathogenic mechanisms. Furthermore, this study lays the foundations for several lines of investigation and the proposed model can also have remarkable therapeutic implications for the assessment of effectiveness and tolerability of new pharmacological compounds. Advisors/Committee Members: tutor: G. P. Comi, co-tutor: A. Di Fonzo, coordinatore: R. Ghidoni, COMI, GIACOMO PIETRO, GHIDONI, RICCARDO.

Subjects/Keywords: Settore MED/26 - Neurologia

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

COMPAGNONI, G. M. (2018). ASSESSING THE PATHOGENESIS OF MULTIPLE SYSTEM ATROPHY THROUGH CELLULAR MODELS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/540359

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

COMPAGNONI, G. MONZIO. “ASSESSING THE PATHOGENESIS OF MULTIPLE SYSTEM ATROPHY THROUGH CELLULAR MODELS.” 2018. Thesis, Università degli Studi di Milano. Accessed February 27, 2021. http://hdl.handle.net/2434/540359.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

COMPAGNONI, G. MONZIO. “ASSESSING THE PATHOGENESIS OF MULTIPLE SYSTEM ATROPHY THROUGH CELLULAR MODELS.” 2018. Web. 27 Feb 2021.

Vancouver:

COMPAGNONI GM. ASSESSING THE PATHOGENESIS OF MULTIPLE SYSTEM ATROPHY THROUGH CELLULAR MODELS. [Internet] [Thesis]. Università degli Studi di Milano; 2018. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2434/540359.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

COMPAGNONI GM. ASSESSING THE PATHOGENESIS OF MULTIPLE SYSTEM ATROPHY THROUGH CELLULAR MODELS. [Thesis]. Università degli Studi di Milano; 2018. Available from: http://hdl.handle.net/2434/540359

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

5. R. Bucci. CONSTRAINED BETA-AMINO ACIDS AS MOLECULAR TOOLS FOR THE PREPARATION OF FOLDAMERS.

Degree: 2018, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/540229

► In these three years, as PhD student, I was involved in different projects regarding the synthesis of new enantiopure beta-amino acids, their use for the… (more)

Subjects/Keywords: Settore CHIM/06 - Chimica Organica

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APA (6^th Edition):

Bucci, R. (2018). CONSTRAINED BETA-AMINO ACIDS AS MOLECULAR TOOLS FOR THE PREPARATION OF FOLDAMERS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/540229

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bucci, R.. “CONSTRAINED BETA-AMINO ACIDS AS MOLECULAR TOOLS FOR THE PREPARATION OF FOLDAMERS.” 2018. Thesis, Università degli Studi di Milano. Accessed February 27, 2021. http://hdl.handle.net/2434/540229.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bucci, R.. “CONSTRAINED BETA-AMINO ACIDS AS MOLECULAR TOOLS FOR THE PREPARATION OF FOLDAMERS.” 2018. Web. 27 Feb 2021.

Vancouver:

Bucci R. CONSTRAINED BETA-AMINO ACIDS AS MOLECULAR TOOLS FOR THE PREPARATION OF FOLDAMERS. [Internet] [Thesis]. Università degli Studi di Milano; 2018. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2434/540229.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bucci R. CONSTRAINED BETA-AMINO ACIDS AS MOLECULAR TOOLS FOR THE PREPARATION OF FOLDAMERS. [Thesis]. Università degli Studi di Milano; 2018. Available from: http://hdl.handle.net/2434/540229

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

6. M. Siano. DIAGNOSTICS OF PARTIALLY COHERENT RADIATION FROM ULTRARELATIVISTIC BEAMS: THE HETERODYNE NEAR FIELD SPECKLE APPROACH.

Degree: 2017, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/540361

► X-ray radiation from relativistic charged particle beams has nowadays achieved unprecedented high brilliance and high degree of coherence, fostering the development of coherence-based techniques such… (more)

▼ X-ray radiation from relativistic charged particle beams has nowadays achieved unprecedented high brilliance and high degree of coherence, fostering the development of coherence-based techniques such as coherent diffractive imaging, photon correlation spectroscopy and phase contrast imaging. Probing the spatial and temporal coherence properties of the X-ray beam is of outermost importance for proper planning of the experiments and for unbiased data reduction. It also paves the way to non-invasive diagnostics techniques aimed at gauging the transverse emittance of the particle beam. Consequently, coherence diagnostics of the radiation emitted in third- and fourth-generation light sources represents an increasingly challenging demand. In this thesis we describe a novel coherence diagnostics technique named Heterodyne Near Field Speckle (HNFS). It exploits the staggering self-referenced interference between the weak spherical waves scattered by nanoparticles in a colloidal suspension and the strong transmitted field to probe the coherence properties of the radiation by means of Fourier analysis of the resulting stochastic speckle fields. A criterion based on master curves from data acquired at different sample-detector distances is introduced, allowing to gauge spatial and temporal coherence separately by means of two different scaling laws. The technique requires a minimal setup and it is ultimately wavelength independent. It can be operated also at X-ray wavelengths, remarkably without any dedicated optics. We first show results obtained with visible table-top thermal sources, validating the master curve criterion and introducing the concept of Analogical Optical Modeling. Then we provide a characterization of the spatio-temporal coherence properties of a pulsed High Harmonic Generation laser at the ELETTRA synchrotron (Trieste, Italy). Finally, the technique is applied to measure the coherence properties of visible synchrotron radiation and of soft X-ray undulator radiation at the ALBA facility (Barcelona, Spain), showing how the technique can be effectively implemented as a valuable beam diagnostics tool. Advisors/Committee Members: tutor: M. A. C. Potenza, supervisore: B. Paroli, POTENZA, MARCO ALBERTO CARLO, PAROLI, BRUNO, POTENZA, MARCO ALBERTO CARLO.

Subjects/Keywords: Coherence; Partial Coherence; Speckle; Synchrotron Radiation; Undulator Radiation; Settore FIS/03 - Fisica della Materia

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Siano, M. (2017). DIAGNOSTICS OF PARTIALLY COHERENT RADIATION FROM ULTRARELATIVISTIC BEAMS: THE HETERODYNE NEAR FIELD SPECKLE APPROACH. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/540361

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Siano, M.. “DIAGNOSTICS OF PARTIALLY COHERENT RADIATION FROM ULTRARELATIVISTIC BEAMS: THE HETERODYNE NEAR FIELD SPECKLE APPROACH.” 2017. Thesis, Università degli Studi di Milano. Accessed February 27, 2021. http://hdl.handle.net/2434/540361.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Siano, M.. “DIAGNOSTICS OF PARTIALLY COHERENT RADIATION FROM ULTRARELATIVISTIC BEAMS: THE HETERODYNE NEAR FIELD SPECKLE APPROACH.” 2017. Web. 27 Feb 2021.

Vancouver:

Siano M. DIAGNOSTICS OF PARTIALLY COHERENT RADIATION FROM ULTRARELATIVISTIC BEAMS: THE HETERODYNE NEAR FIELD SPECKLE APPROACH. [Internet] [Thesis]. Università degli Studi di Milano; 2017. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2434/540361.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Siano M. DIAGNOSTICS OF PARTIALLY COHERENT RADIATION FROM ULTRARELATIVISTIC BEAMS: THE HETERODYNE NEAR FIELD SPECKLE APPROACH. [Thesis]. Università degli Studi di Milano; 2017. Available from: http://hdl.handle.net/2434/540361

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

7. G. Aiello. NUTRITIONAL PEPTIDOMICS: DISCOVERY, QUANTIFICATION, AND FUNCTIONAL ANALYSIS OF PLANT PROTEIN DERIVED PEPTIDES.

Degree: 2018, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/540245

► NUTRITIONAL PEPTIDOMICS: DISCOVERY, QUANTIFICATION, AND FUNCTIONAL ANALYSIS OF PLANT PROTEIN DERIVED PEPTIDES Sector CHIM/10 - Food chemistry Introduction and aims of thesis The study of… (more)

▼ NUTRITIONAL PEPTIDOMICS: DISCOVERY, QUANTIFICATION, AND FUNCTIONAL ANALYSIS OF PLANT PROTEIN DERIVED PEPTIDES Sector CHIM/10 - Food chemistry Introduction and aims of thesis The study of bioactive peptides is a central issue in the development of innovative therapies. The increased attention for fresher and ‘greener’ foods and nutraceuticals possessing health-preventing or health-promoting properties makes bioactive peptides suitable candidates for a new era of pharmaceutical products. Analysing and understanding nature and bioactivity of nutritional peptides, typically delivered from parent food proteins, means comprehending an important level of environmental regulation of the human genome: diet is the environmental factor having the most profound life-long influence on health. Although a remarkable progress has been done in protein analysis, as a consequence of proteomic research, and in small molecule analysis, as a consequence of drug discovery/development initiatives, the field of nutritional peptidomic is still quite unexplored and some drawbacks should be addressed. The pharmacological applications of bioactive peptides depend primarily on their ability to be absorbed in order to exert their bioactivity. In addition, it is very likely that peptide sequences are subjected to structural alterations before performing their final activity in vivo due to different events, such as the attack of gastrointestinal enzymes, brush border peptidases, absorption through the intestinal barrier, and attack of intracellular peptidases in the intracellular absorption. Therefore, all of these different aspects about the bioavailability have attracted a growing interest in the last years. In addition, the possibility of the peptides breakdown during the gastrointestinal digestion is one of the most important factor to be considered when evaluating food-derived peptides for the promotion of human health. Chemical stability is also crucial for proper assay development, since these peptides could lose stability when placed in solution or in biological fluids or even before absorption. Once they are delivered, the biodistribution of bioactive peptides may be sometimes hampered as a result of proteolytic attack, primarily due to the action of brush border peptidases overexpressed at the microvilli surface of intestinal cells. However, before evaluating aspects such as the bioavailability, the optimization of hydrolytic conditions and the chemical identification of protein hydrolysates are other important aspects to be highlighted. The broad variety of physiological activities attributed to protein hydrolysates are determined by the type, number, position, and properties of amino acids present in the sequence of bioactive peptides. The optimization of the hydrolytic conditions for the obtainment of bioactive hydrolysates was also addressed in this work. Since peptides derive from proteins, the integration of peptidomics and proteomics methodologies permitted the enlargement of proteomic databank, which may facilitate… Advisors/Committee Members: faculty advisor: A. Arnoldi, phd coordinator: G. Aldini, ARNOLDI, ANNA, ALDINI, GIANCARLO.

Subjects/Keywords: PEPTIDOMICS; BIOACTIVE PEPTIDES; PLANT PROTEIN; MASS SPECTROMETRY; Settore CHIM/10 - Chimica degli Alimenti

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APA (6^th Edition):

Aiello, G. (2018). NUTRITIONAL PEPTIDOMICS: DISCOVERY, QUANTIFICATION, AND FUNCTIONAL ANALYSIS OF PLANT PROTEIN DERIVED PEPTIDES. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/540245

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Aiello, G.. “NUTRITIONAL PEPTIDOMICS: DISCOVERY, QUANTIFICATION, AND FUNCTIONAL ANALYSIS OF PLANT PROTEIN DERIVED PEPTIDES.” 2018. Thesis, Università degli Studi di Milano. Accessed February 27, 2021. http://hdl.handle.net/2434/540245.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Aiello, G.. “NUTRITIONAL PEPTIDOMICS: DISCOVERY, QUANTIFICATION, AND FUNCTIONAL ANALYSIS OF PLANT PROTEIN DERIVED PEPTIDES.” 2018. Web. 27 Feb 2021.

Vancouver:

Aiello G. NUTRITIONAL PEPTIDOMICS: DISCOVERY, QUANTIFICATION, AND FUNCTIONAL ANALYSIS OF PLANT PROTEIN DERIVED PEPTIDES. [Internet] [Thesis]. Università degli Studi di Milano; 2018. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2434/540245.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Aiello G. NUTRITIONAL PEPTIDOMICS: DISCOVERY, QUANTIFICATION, AND FUNCTIONAL ANALYSIS OF PLANT PROTEIN DERIVED PEPTIDES. [Thesis]. Università degli Studi di Milano; 2018. Available from: http://hdl.handle.net/2434/540245

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

8. G. Baron. MASS SPECTROMETRIC STRATEGIES FOR THE STUDY OF PLANT EXTRACTS BIOAVAILABILITY, BIOACTIVITY AND MECHANISMS OF ACTION.

Degree: 2018, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/540325

► 1. Introduction and aim of the work Drug discovery in phytomedicine has in the past been mainly focused on the isolation and characterization of new… (more)

▼ 1. Introduction and aim of the work Drug discovery in phytomedicine has in the past been mainly focused on the isolation and characterization of new bioactive compounds from natural products. Several NCE (new chemical entities) have been isolated from plants and they are now the active principles of many drugs able to treat and prevent different kinds of diseases. This drug discovery approach is aimed at the determination of the single "active principle" in plants, based on the assumption that a plant has one or more ingredients which determine its therapeutic effects. Beside NCE derived from plants and herbs, there is another important approach which assumes that a synergy of all ingredients of plants will bring about the maximum of therapeutic efficacy [1]. There are new forms of registered plant-derived medicines (phytomedicines) that are not single chemical entities but a complex mixture of active and inert ingredients derived form a crude extraction. However this approach has long been limited since adequate methods to standardize complex plant mixtures as well as to rationalize complex modes of actions were lacking. Moreover ADMET studies were limited due to the complexity of the phytomedicines. Hence most of the information that is usually retrieved for NCE during the drug discovery stage, such as the ADME profile and the mechanism of action was often not obtained for such complex natural derivatives, limiting their efficacy and application in therapy. Recently, thanks to the advent of novel MS techniques and to the commercial availability of high resolution MS analysers, the opportunity to determine the ADME profiles of plant extracts and to explore their mode of action has become possible. Advanced analytical techniques play an increasingly important role in the characterization, identification and quantification of plant extract compounds, not only in the context of their natural source but also in biological fluids to study their bioavailability and to discover the active compounds. Mass spectrometry has become one of the main standard techniques in this field because of the high sensitivity and specificity of the available mass analyzers. Based on these premises, the aim of my PhD work has been to set-up and apply state of the art MS strategies to better understand the mechanisms of action of some crude plant extracts and in particular tannins and rice extracts as well as to define the absorption and PK profile of cranberry and bilberry standardized extracts which are widely used as therapeutic agents. 2. Set-up and application of MS methods to elucidate biological activities and mechanisms of action of plant extracts During the first part of my Ph.D program, I have used MS strategies to investigate the ability of plant extracts to act as i) sequestering agents of reactive carbonyl species, toxic lipid peroxidation products involved in the pathogenetic mechanisms of several inflammatory based disorders and ii) as protein precipitation agents (tannin effect) using bradykinin, a… Advisors/Committee Members: tutor: G. Aldini, coordinator: G. Aldini, ALDINI, GIANCARLO, ALDINI, GIANCARLO.

Subjects/Keywords: Plant extract; ADME; mass spectrometry; Settore CHIM/08 - Chimica Farmaceutica

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Baron, G. (2018). MASS SPECTROMETRIC STRATEGIES FOR THE STUDY OF PLANT EXTRACTS BIOAVAILABILITY, BIOACTIVITY AND MECHANISMS OF ACTION. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/540325

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Baron, G.. “MASS SPECTROMETRIC STRATEGIES FOR THE STUDY OF PLANT EXTRACTS BIOAVAILABILITY, BIOACTIVITY AND MECHANISMS OF ACTION.” 2018. Thesis, Università degli Studi di Milano. Accessed February 27, 2021. http://hdl.handle.net/2434/540325.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Baron, G.. “MASS SPECTROMETRIC STRATEGIES FOR THE STUDY OF PLANT EXTRACTS BIOAVAILABILITY, BIOACTIVITY AND MECHANISMS OF ACTION.” 2018. Web. 27 Feb 2021.

Vancouver:

Baron G. MASS SPECTROMETRIC STRATEGIES FOR THE STUDY OF PLANT EXTRACTS BIOAVAILABILITY, BIOACTIVITY AND MECHANISMS OF ACTION. [Internet] [Thesis]. Università degli Studi di Milano; 2018. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2434/540325.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Baron G. MASS SPECTROMETRIC STRATEGIES FOR THE STUDY OF PLANT EXTRACTS BIOAVAILABILITY, BIOACTIVITY AND MECHANISMS OF ACTION. [Thesis]. Università degli Studi di Milano; 2018. Available from: http://hdl.handle.net/2434/540325

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

9. B. Guidi. ENZYMES FROM MARINE MICROORGANISMS FOR THE PREPARATION OF BIOLOGICALLY ACTIVE MOLECULES.

Degree: 2018, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/541057

► This PhD project focuses on the identification, isolation and characterization of new biocatalysts able to generate biologically active molecules with significant enantioselectivity. Through screening, we… (more)

▼ This PhD project focuses on the identification, isolation and characterization of new biocatalysts able to generate biologically active molecules with significant enantioselectivity. Through screening, we identified marine strains, from MaCuMBA (Marine Culturable Microorganism for Biotechnological Applications) and BIODEEP (Biotechnologies form the deep) European project collections, which show a marked enantioselectivity on intermediates of molecules of biological interest. Biotransformation substrate range included pramipexole, as main target, but it also embraces other common building blocks for synthetic industrial preparation. The stereoselective reduction of structurally different ketones using halotolerant marine yeasts (Meyerozyma guilliermondii and Rhodotorula mucilaginosa) was studied using cells grown and bio-converted in seawater. The preparation of valuable chemicals through water-saving (bio)processes based on the direct exploitation of seawater is a significant step towards sustainable biocatalysis. By choosing a suitable strain, high yields and stereoselectivity could be achieved in most cases. Notably, high chemoselectivity and enantioselectivity were observed using R. mucilaginosa in the reduction of aromatic β-ketonitriles, which allowed the recovery of the optically pure corresponding alcohols; notably, reduction with whole cells of yeasts generally give a mixture of undesired products, as observed with M. guilliermondii. Keto-reduction potential of thirty-three marine bacterium species was checked and afterwards the possibility to convert this substrate directly into the optically pure amine was investigated: marine bacteria were screened to identify transaminase activity. Based on the previous results in terms of halotolerance and transaminase activity, the marine bacterium strain Virgibacillus pantothenticus 21D was selected for the genome sequencing in order to clone and express an ω-transaminase enzyme. A recombinant non-marine ketoreductase from Pichia glucozyma (KRED1-Pglu) was used for the enantioselective reduction of various cyclic ketones including pramipexole ketone intermediate. Thanks to co-factor recycling system, the purified enzyme showed very promising results. The soluble expression of a novel omega transaminase from a newly isolated halotolerant marine bacterium Virgibacillus pantothenticus was attained. Despite of several standard methodologies applied, the marine wild-type enzyme was total insoluble in E. coli host and it was satisfactorily solubilized by one single-point mutation, allowing the characterization of the new omega transaminase. The enzyme shows an interesting salt and solvent tolerance, in accordance to its origin and it results particularly active on some interesting building blocks molecules. Advisors/Committee Members: docente guida: P. Ferraboschi, direttore del corso di dottorato: S. Sonnino, FERRABOSCHI, PATRIZIA, SONNINO, SANDRO.

Subjects/Keywords: Biocatalysis; marine bacteria; marine yeasts; transaminase; protein engeneering; halotolerance; Settore BIO/10 - Biochimica; Settore CHIM/11 - Chimica e Biotecnologia delle Fermentazioni

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Guidi, B. (2018). ENZYMES FROM MARINE MICROORGANISMS FOR THE PREPARATION OF BIOLOGICALLY ACTIVE MOLECULES. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/541057

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Guidi, B.. “ENZYMES FROM MARINE MICROORGANISMS FOR THE PREPARATION OF BIOLOGICALLY ACTIVE MOLECULES.” 2018. Thesis, Università degli Studi di Milano. Accessed February 27, 2021. http://hdl.handle.net/2434/541057.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Guidi, B.. “ENZYMES FROM MARINE MICROORGANISMS FOR THE PREPARATION OF BIOLOGICALLY ACTIVE MOLECULES.” 2018. Web. 27 Feb 2021.

Vancouver:

Guidi B. ENZYMES FROM MARINE MICROORGANISMS FOR THE PREPARATION OF BIOLOGICALLY ACTIVE MOLECULES. [Internet] [Thesis]. Università degli Studi di Milano; 2018. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2434/541057.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Guidi B. ENZYMES FROM MARINE MICROORGANISMS FOR THE PREPARATION OF BIOLOGICALLY ACTIVE MOLECULES. [Thesis]. Università degli Studi di Milano; 2018. Available from: http://hdl.handle.net/2434/541057

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

10. G. Gargari. EVALUATION OF THE IMPACT OF DIETARY INTERVENTIONS ON THE HUMAN INTESTINAL MICROBIAL ECOSYSTEM THROUGH IMPROVED BIOINFORMATICS AND STATISTICS.

Degree: 2017, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/541101

► The aim of this PhD work was the characterization of the intestinal microbial ecosystem through bioinformatic and statistical analyses of the microbiomics data originated from… (more)

▼ The aim of this PhD work was the characterization of the intestinal microbial ecosystem through bioinformatic and statistical analyses of the microbiomics data originated from three studies carried out on different human populations: healthy (non-diseased) adults, hyperlipidemic and normo-lipidemic children and adolescents, and subjects with diagnosed irritable bowel syndrome (IBS). Specifically, the three studies presented in this PhD theses were as follows: • Probiotic crossover intervention study: A randomized, double-blind, crossover, placebo-controlled intervention study was carried out to determine the impact of a Bifidobacterium bifidum strain on the IME of adult healthy volunteers. • Children’s dyslipidemia single arm intervention study: In this trial, the IME of children and adolescents with primary hyperlipidemia (a risk factor for cardiovascular diseases) was compared with the IME of control (normo-lipidemic) group. In addition, the modulatory effect of the regular intake of hazelnuts on hyperlipidemic subjects’s IME was examines in order to evaluate if the modulation of IME by hazelnuts ameliorates the lipid profile. • Irritable bowel syndrome (IBS) observational study: The aim of this trial was the characterization of the IME in human subjects affected by IBS. The characterization was performed through the analysis of the bacterial communities present in the faecal samples collected during a multicentre intervention trial (Cremon et al., 2017). Irritable bowel syndrome is the most common gastrointestinal disorder in western countries. IBS is conventionally classified in four different subtypes based on bowel clinical symptoms: constipation, diarrhoea, alternating constipation and diarrhoea, and unsubtyped IBS. Clinical and immunological data collected during the trial were used to investigate the potential correlations between the IME characteristic for a specific IBS subtypes and the physiological and clinical parameters of the host, including bowel symptoms, faecal levels of IgA and cytokines, and depression/anxiety scores. The analysis of the data collected during the three studies presented in this PhD thesis revealed significant correlations between the intestinal microbial ecosystem and specific physiological parameters, characteristic for the physiological conditions under study. Therefore, these findings suggest that the differential abundance of specific OTUs could be used as a biomarker for a specific host condition. Conclusion of the probiotic B. bifidum Bb crossover intervention trial The intervention trial based on the administration of B. bifidum strain Bb has shown that the probiotic treatment modified the relative abundances of bacterial taxa that have often been associated with healthy conditions. The treatment modulated the faecal levels of butyrate, a microbial metabolite exerting multiple effects on gut health. Therefore, the daily consumption of B. bifidum Bb cells may positively affect human health; however, as for most dietary interventions, the current state of… Advisors/Committee Members: tutor: S. Guglielmetti, phd dean: F. Bonomi, GUGLIELMETTI, SIMONE DOMENICO, BONOMI, FRANCESCO.

Subjects/Keywords: Bifidumbacterium bifidum; hyperlipidemia; IBS; SCFAs; microbiota; IME; Settore AGR/16 - Microbiologia Agraria

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gargari, G. (2017). EVALUATION OF THE IMPACT OF DIETARY INTERVENTIONS ON THE HUMAN INTESTINAL MICROBIAL ECOSYSTEM THROUGH IMPROVED BIOINFORMATICS AND STATISTICS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/541101

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gargari, G.. “EVALUATION OF THE IMPACT OF DIETARY INTERVENTIONS ON THE HUMAN INTESTINAL MICROBIAL ECOSYSTEM THROUGH IMPROVED BIOINFORMATICS AND STATISTICS.” 2017. Thesis, Università degli Studi di Milano. Accessed February 27, 2021. http://hdl.handle.net/2434/541101.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gargari, G.. “EVALUATION OF THE IMPACT OF DIETARY INTERVENTIONS ON THE HUMAN INTESTINAL MICROBIAL ECOSYSTEM THROUGH IMPROVED BIOINFORMATICS AND STATISTICS.” 2017. Web. 27 Feb 2021.

Vancouver:

Gargari G. EVALUATION OF THE IMPACT OF DIETARY INTERVENTIONS ON THE HUMAN INTESTINAL MICROBIAL ECOSYSTEM THROUGH IMPROVED BIOINFORMATICS AND STATISTICS. [Internet] [Thesis]. Università degli Studi di Milano; 2017. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2434/541101.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gargari G. EVALUATION OF THE IMPACT OF DIETARY INTERVENTIONS ON THE HUMAN INTESTINAL MICROBIAL ECOSYSTEM THROUGH IMPROVED BIOINFORMATICS AND STATISTICS. [Thesis]. Università degli Studi di Milano; 2017. Available from: http://hdl.handle.net/2434/541101

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

11. F. Valdetara. STUDY OF THE VOLATILE PHENOL METABOLISM AND ROLE OF SO2 AS STRESS AGENT IN BRETTANOMYCES/DEKKERA BRUXELLENSIS UNDER WINE CONDITIONS.

Degree: 2017, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/541053

► Biological spoilage of wine arises from yeasts and bacteria metabolic activity. Brettanomyces/Dekkera bruxellensis, one of the main contaminating yeast, is able to produce unpleasant compounds,… (more)

▼ Biological spoilage of wine arises from yeasts and bacteria metabolic activity. Brettanomyces/Dekkera bruxellensis, one of the main contaminating yeast, is able to produce unpleasant compounds, such as vinyl and ethyl phenols (VPs). These off-flavors define the so called “Brett” character. Two enzymes, the cinnamate decarboxylase (CD) and the vinylphenol reductase (VPR), are involved in the spoilage activity. Sulphur dioxide (SO2) is the most common additive used to prevent and/or control microbial contamination in many foods, but decreasing its use is advisable both for limiting the detrimental cumulative effects on human health and improving the sustainability in winemaking. The first aim of this study was to provide a certain identification of the VPR enzyme, by cloning the gene in a species not producing ethyl phenols, such as Saccharomyces cerevisiae. The role of this enzyme in the conversion of 4-vinyl guaiacol into 4-ethyl guaiacol was proven by the expression, of a biologically active form of the heterologous protein. A VPR specific activity of 9 ± 0.6 mU/mg is found in crude extracts of transformed clones of S. cerevisiae. A his-tag purification approach allowed to confirm the results in activity trials carried out in the enriched fraction of the protein purified from recombinant cells of S. cerevisiae; in particular, a VPR specific activity of 1.83 ± 0.03 U/mg at pH 6.0 is measured. Furthermore, the strain-dependent character of the species regarding the VP production was investigated at sequence level in 17 different D. bruxellensis strains. Since the observed polymorphism (2.3%) and the allelic heterozygosity state of the gene do not correlate with the different release in off-flavors, this could indicate that transcriptional/post-translational mechanisms might affect the final production. In addition, the expression of the two genes involved in VP production was investigated as a three-factor variation response using a Response Surface Methodology approach. As first, a proper house-keeping-gene was identified to allow the analysis of different SO2, pH and ethanol concentrations on VP production under oenological conditions. While statistical irrelevance as far SO2 lead this to not be commented as main factor affecting CD expression, the linear interaction with pH and ethanol concurr to define a significant effect (p < 0.05) on it. Considering the permissive growth condition (0 mg/L mol.SO2, pH 4.5 and 5% v/v EtOH), CD is generally downregulated. The combination of factor levels maximizing (0.83 fold-change) CD expression is: 0.25 mg/L mol. SO2, pH 4.5 and 12.5% v/v EtOH. Contrariwise, VPR expression does not seem to be influenced by any main factor nor by their interactions, but its expression is maximized (1.80 fold-change) at the same conditions calculated for CD gene. Finally, the study of the genetic mechanisms involved in the SO2 stress response of two B./D. bruxellensis strains (AWRI1499 and CBS2499) was carried out. In particular, a RNA-Seq based approach was applied on cells grew in a wine-model… Advisors/Committee Members: tutor: I. Vigentini, co-tutor: R. Foschino, coordinatore: F. Bonomi, VIGENTINI, ILEANA, BONOMI, FRANCESCO.

Subjects/Keywords: Brettanomyces/Dekkera bruxellensis; sulphur dioxide; RNA-seq; volatile phenols; wine; Settore AGR/16 - Microbiologia Agraria

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Valdetara, F. (2017). STUDY OF THE VOLATILE PHENOL METABOLISM AND ROLE OF SO2 AS STRESS AGENT IN BRETTANOMYCES/DEKKERA BRUXELLENSIS UNDER WINE CONDITIONS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/541053

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Valdetara, F.. “STUDY OF THE VOLATILE PHENOL METABOLISM AND ROLE OF SO2 AS STRESS AGENT IN BRETTANOMYCES/DEKKERA BRUXELLENSIS UNDER WINE CONDITIONS.” 2017. Thesis, Università degli Studi di Milano. Accessed February 27, 2021. http://hdl.handle.net/2434/541053.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Valdetara, F.. “STUDY OF THE VOLATILE PHENOL METABOLISM AND ROLE OF SO2 AS STRESS AGENT IN BRETTANOMYCES/DEKKERA BRUXELLENSIS UNDER WINE CONDITIONS.” 2017. Web. 27 Feb 2021.

Vancouver:

Valdetara F. STUDY OF THE VOLATILE PHENOL METABOLISM AND ROLE OF SO2 AS STRESS AGENT IN BRETTANOMYCES/DEKKERA BRUXELLENSIS UNDER WINE CONDITIONS. [Internet] [Thesis]. Università degli Studi di Milano; 2017. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2434/541053.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Valdetara F. STUDY OF THE VOLATILE PHENOL METABOLISM AND ROLE OF SO2 AS STRESS AGENT IN BRETTANOMYCES/DEKKERA BRUXELLENSIS UNDER WINE CONDITIONS. [Thesis]. Università degli Studi di Milano; 2017. Available from: http://hdl.handle.net/2434/541053

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

12. V. DE VITIS. NEW INTEGRATED APPROACHES FOR THE DEVELOPMENT OF BIOCATALYTICAL PROCESSES.

Degree: 2017, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/541055

►

Questa tesi di Dottorato è stata strutturata in tre parti, una per ogni tipo di attività enzimatica trattata, preceduta da una breve introduzione riguardante i… (more)

▼

Questa tesi di Dottorato è stata strutturata in tre parti, una per ogni tipo di attività enzimatica trattata, preceduta da una breve introduzione riguardante i principi fondamentali della biocatalisi. Parte 1: Sono state studiate le caratteristiche strutturali e biochimiche dell’esterasi (BCE) prodotta per via ricombinante da Bacillus coagulans NCBI 9365. Questo enzima mostra un elevato grado di selettività nei confronti degli esteri (acetato, butirrato e benzoato) dell’1,2-O-isopropilidenglicerolo (IPG) un acetale equivalente chirale del glicerolo, che in entrambe le configurazioni, è un utile prodotto di partenza per la sintesi di molecole naturali biologicamente attive di interesse alimentare e farmaceutico come gliceridi, glicerofosfolipidi, -bloccanti ed antiipertensivi Parte 2: Sono state studiate le potenzialità biocatalitiche di batteri acetici, microrganismi noti per la loro capacità di accumulare prodotti di ossidazione (aldeidi, chetoni e acidi carbossilici) dati dalla trasformazione di alcoli primari e secondari. Questa parte risulta ulteriormente suddivisa in tre capitoli: • La desimmetrizzazione stereoselettiva di dioli achirali diversamente sostituiti, è stata eseguita ottenendo i corrispettivi acidi chirali utilizzando, cellule intere di Acetobacter aceti MIM 2000/28 • È stata studiata la sintesi chemo-enzimatica della forma enantiopura del captopril, noto farmaco utilizzato per il trattamento dell’ipertensione. Particolare attenzione è stata dedicata allo step enzimatico catalizzato da cellule immobilizzate di Acetobacter aceti MIM 2000/28 utilizzate in un sistema in continuo di “flow chemistry”. • La produttività e la stabilità del sistema di ossidazione “flow chemistry” è stato studiato e ottimizzata attraverso l’utilizzo del 2-metil 1,3 propandiolo. Per testare la reale efficienza del sistema in continuo messo a punto, sono in corso di studio le prove effettuate con ulteriori substrati. Parte 3: L'idrossilazione del (R) -limonene in (R)-alcool-perililico è stata studiata attraverso l’utilizzo di cellule intere ricombinanti di E. coli trasformate con l’operone sintetico ottimizzato che codifica per una citocromo P450 monossigenasi (CYP153A6), una ferredossina e una ferredossina reduttasi isolate dal ceppo Mycobacterium sp. HXN-1500

This PhD project has been structured into three parts preceded by a general introduction on basic biocatalysis concepts. Part 1: The recombinant preparation coupled with biochemical and structure characterization a thermostable carboxylesterase (BCE) from Bacillus coagulans NCBI 9365. This enzyme showed high enantioselectivity towards different 1,2-O-isopropylideneglycerol esters (acetate, butyrate and benzoate). Both enantiopure of 1,2-O-isopropylideneglycerol (IPG or solketal), a chiral equivalent of glycerol, are building blocks for the synthesis of -blockers, glycerophospholipids and prostaglandins. Part 2: An insight on the biocatalytic potential of Acetic Acid Bacteria (AAB), a group of bacteria well-known for the ability to oxidize primary and…

Advisors/Committee Members: docente guida: F. Molinari, direttore del corso di dottorato: F. Bonomi, MOLINARI, FRANCESCO ENZO, BONOMI, FRANCESCO.

Subjects/Keywords: biocatalysis, Esterase, Flow chemistry, Limonene, Acetic acid bacteria, selective oxidation; Settore CHIM/11 - Chimica e Biotecnologia delle Fermentazioni; Settore AGR/16 - Microbiologia Agraria

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

VITIS, V. D. (2017). NEW INTEGRATED APPROACHES FOR THE DEVELOPMENT OF BIOCATALYTICAL PROCESSES. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/541055

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

VITIS, V. DE. “NEW INTEGRATED APPROACHES FOR THE DEVELOPMENT OF BIOCATALYTICAL PROCESSES.” 2017. Thesis, Università degli Studi di Milano. Accessed February 27, 2021. http://hdl.handle.net/2434/541055.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

VITIS, V. DE. “NEW INTEGRATED APPROACHES FOR THE DEVELOPMENT OF BIOCATALYTICAL PROCESSES.” 2017. Web. 27 Feb 2021.

Vancouver:

VITIS VD. NEW INTEGRATED APPROACHES FOR THE DEVELOPMENT OF BIOCATALYTICAL PROCESSES. [Internet] [Thesis]. Università degli Studi di Milano; 2017. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2434/541055.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

VITIS VD. NEW INTEGRATED APPROACHES FOR THE DEVELOPMENT OF BIOCATALYTICAL PROCESSES. [Thesis]. Università degli Studi di Milano; 2017. Available from: http://hdl.handle.net/2434/541055

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

13. L. Vergani. BIOREMEDIATION OF A POLYCHLORINATED BIPHENYL (PCB) POLLUTED SITE: DEGRADING POTENTIAL OF SOIL MICROBIOTA AND EXPLOITATION OF PLANT-BACTERIA INTERACTIONS FOR ENHANCED RHIZOREMEDIATION.

Degree: 2017, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/540645

► The release of xenobiotic chemicals into the environment has dramatically increased over the last century following industrialization, with a consequent impact on the ecosystems and… (more)

▼ The release of xenobiotic chemicals into the environment has dramatically increased over the last century following industrialization, with a consequent impact on the ecosystems and human health. Polychlorinated biphenyls (PCB), in particular, are among the twelve chlorinated organic compound families initially listed as persistent organic pollutants (POPs) by the Stockholm Convention on POPs. PCB, due to their chemical properties and high stability, have been widely used by industries in the twentieth century as dielectric and coolant fluids. Despite their production has been banned since the 1970s-1980s, these pollutants contaminate soils and waters and affect the ecosystems worldwide, being widespread global contaminants. Due to their high lipophilicity, PCB are recalcitrant to biodegradation, persist in the environment and bioaccumulate in the lipids of animals and humans, biomagnifying in the food web. It has been proved that PCB have relevant toxic effects on human health, including carcinogenic activity. The remediation of PCB-contaminated soils represents therefore a primary issue for our society; nonetheless, the available physical-chemical technologies have strong environmental and economic impact and are unsuitable for in situ soil remediation in extended contaminated areas. Rhizoremediation is a type of phytoremediation that relies on the capability of soil microbes responding to plant biostimulation, to degrade pollutants. This strategy appears as the most suitable for the detoxification of large-scale PCB-polluted soils. Among soil contaminants, rhizoremediation of PCB is specifically relying on the positive interactions between plants and microorganisms in the rhizosphere. In fact, several organic aromatic compounds released through root deposition can promote the activation of the biphenyl catabolic pathway that is responsible for the microbial oxidative PCB metabolism, thereby improving the overall PCB degradation performance in aerobic conditions in soil. Moreover, plant-growth promoting (PGP) microorganisms selected in the rhizosphere can sustain plant growth under stressed conditions typical of polluted soils, in turn enhancing the plant biostimulation. Nevertheless, the efficiency of this biotechnology in situ has been poorly assessed in the scientific literature, since the upscaling from laboratory to greenhouse conditions to the field was rarely implemented. Site-specific environmental conditions still represent a major challenge for an efficient in situ rhizoremediation intervention, especially when it comes to understand how the pollution fingerprint affects the autochthonous degrading bacterial populations and whether these are able to establish positive interactions with the introduced plant species. This PhD project focused on the Site of National Priority (SIN) Caffaro, a large site located in Northern Italy historically polluted by chlorinated POPs and metals. Aim of the work was to study the phylogenetic and functional diversity of the soil microbiota, assessing the correlation between… Advisors/Committee Members: supervisor: S. Borin, coordinatore: F. Bonomi, BORIN, SARA, BONOMI, FRANCESCO.

Subjects/Keywords: Settore AGR/16 - Microbiologia Agraria

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vergani, L. (2017). BIOREMEDIATION OF A POLYCHLORINATED BIPHENYL (PCB) POLLUTED SITE: DEGRADING POTENTIAL OF SOIL MICROBIOTA AND EXPLOITATION OF PLANT-BACTERIA INTERACTIONS FOR ENHANCED RHIZOREMEDIATION. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/540645

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Vergani, L.. “BIOREMEDIATION OF A POLYCHLORINATED BIPHENYL (PCB) POLLUTED SITE: DEGRADING POTENTIAL OF SOIL MICROBIOTA AND EXPLOITATION OF PLANT-BACTERIA INTERACTIONS FOR ENHANCED RHIZOREMEDIATION.” 2017. Thesis, Università degli Studi di Milano. Accessed February 27, 2021. http://hdl.handle.net/2434/540645.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vergani, L.. “BIOREMEDIATION OF A POLYCHLORINATED BIPHENYL (PCB) POLLUTED SITE: DEGRADING POTENTIAL OF SOIL MICROBIOTA AND EXPLOITATION OF PLANT-BACTERIA INTERACTIONS FOR ENHANCED RHIZOREMEDIATION.” 2017. Web. 27 Feb 2021.

Vancouver:

Vergani L. BIOREMEDIATION OF A POLYCHLORINATED BIPHENYL (PCB) POLLUTED SITE: DEGRADING POTENTIAL OF SOIL MICROBIOTA AND EXPLOITATION OF PLANT-BACTERIA INTERACTIONS FOR ENHANCED RHIZOREMEDIATION. [Internet] [Thesis]. Università degli Studi di Milano; 2017. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2434/540645.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Vergani L. BIOREMEDIATION OF A POLYCHLORINATED BIPHENYL (PCB) POLLUTED SITE: DEGRADING POTENTIAL OF SOIL MICROBIOTA AND EXPLOITATION OF PLANT-BACTERIA INTERACTIONS FOR ENHANCED RHIZOREMEDIATION. [Thesis]. Università degli Studi di Milano; 2017. Available from: http://hdl.handle.net/2434/540645

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

14. M. Callegari. THE GUT MICROBIOME ASSOCIATED TO HONEYBEES AND WASTE-REDUCING INSECTS.

Degree: 2017, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/540651

► Insects are the most diverse group of animals on Earth and are adapted to a wide range of habitats. They have a remarkable impact on… (more)

▼ Insects are the most diverse group of animals on Earth and are adapted to a wide range of habitats. They have a remarkable impact on the human life: they include not only human and animal parasites, crop pests, or vectors of human, animal and plant diseases, but also beneficial insects, such as pollinators (e.g. the honeybee Apis mellifera), insects reared to obtain products for the human benefit (e.g. silkworms) or mass-reared insects as food and feed or as biological control agents (e.g. parasitoids or predators). Moreover, since the increase of human population, the growing demand for protein for human and animal consumption is forcing the search of alternative sources: in this scenario insects have been proposed as sustainable rich-protein substrates. For instance, the black soldier fly (BSF, Hermetia illucens) is a promising candidate for the sustainable recycling of biological waste into feedstuff for livestock, poultry and aquaculture in the framework of a circular economy approach (Nguyen et al., 2015; van Huis et al., 2013). The insect evolutionary success and diversification are partially due to the symbiotic relationships that they have established with a wide range of microorganisms. These complex symbiotic interactions include commensal, parasitic and mutualistic relationships (Dale and Moran, 2006). The function commonly attributed to the microorganisms that inhabit the intestinal tract of animals is the depolymerization and breakdown of the diet components, which allow the nutrient supplementation and recycling. Moreover, they also provide detoxification of the toxic diet-components and protection against pathogens and parasites and this underlines the importance of a healthy gut microbiota for the host well-being (Dale and Moran, 2006; Engel and Moran, 2013; Hamdi et al., 2011). At the beginning of this PhD thesis an introductive chapter offers an overview of the current knowledge on the potential application of microorganisms in relation to the management of the emerging insect farming with agricultural, industrial and environmental interest. The insect gut microbiota is influenced by many factors, such as the host diet, developmental stage and genetics, and in the last years researchers have been addressed many efforts to elucidate their impact on the host gut microbiota, mainly considering insect pests, parasites or vectors of diseases (Montagna et al., 2015a; Vacchini et al., 2017). Moreover, the importance to characterize factors, such as the oxygen concentration, pH and redox potential status, existing within the insect gut compartments has been only recently recognized and, hence, not so much work has been performed so far in this direction. Indeed, the insect gut includes aerobic and anaerobic niches, passing through microaerophilic habitats, and compartments characterized by acidic, neutral or basic conditions, even in the same digestive tract (Engel and Moran, 2013). Understanding the drivers that shape the microbial diversity in the insect gut microbiota is pivotal in comprehending the… Advisors/Committee Members: supervisor: E. Crotti, coordinatore: F. Bonomi, CROTTI, ELENA SARA, BONOMI, FRANCESCO.

Subjects/Keywords: Settore AGR/16 - Microbiologia Agraria

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Callegari, M. (2017). THE GUT MICROBIOME ASSOCIATED TO HONEYBEES AND WASTE-REDUCING INSECTS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/540651

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Callegari, M.. “THE GUT MICROBIOME ASSOCIATED TO HONEYBEES AND WASTE-REDUCING INSECTS.” 2017. Thesis, Università degli Studi di Milano. Accessed February 27, 2021. http://hdl.handle.net/2434/540651.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Callegari, M.. “THE GUT MICROBIOME ASSOCIATED TO HONEYBEES AND WASTE-REDUCING INSECTS.” 2017. Web. 27 Feb 2021.

Vancouver:

Callegari M. THE GUT MICROBIOME ASSOCIATED TO HONEYBEES AND WASTE-REDUCING INSECTS. [Internet] [Thesis]. Università degli Studi di Milano; 2017. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2434/540651.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Callegari M. THE GUT MICROBIOME ASSOCIATED TO HONEYBEES AND WASTE-REDUCING INSECTS. [Thesis]. Università degli Studi di Milano; 2017. Available from: http://hdl.handle.net/2434/540651

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

15. A. Ciacciulli. FRUIT FLESH IN PEACH:CHARACTERIZATION OF THE 'SLOW SOFTENING' TEXTURE.

Degree: 2018, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/540666

► The aim of this research was to deepen the knowledge about the slow softening texture in peach. The texture is a synthesis of several parameters… (more)

▼ The aim of this research was to deepen the knowledge about the slow softening texture in peach. The texture is a synthesis of several parameters detected by senses, derived from the food structure. The paramount sense in the texture perception is the tactile one, principally perceived by hand and mouth. The tactile perception is a combination of four classes of mechanoreceptors, each one specialized to perceive mechanic deformation with different speed. This combined perception influences the consumer evaluation of food quality, giving the texture importance among food characteristics. The texture could also affect the taste perception through mechanical actions on food structure. The mechanical property linked to the texture is associated with the cellular organization and the cell wall strength. The main cell wall component affecting texture in fresh fruit is pectin, a polymer of galacturonic acid. The disassembly of pectin involves several enzymatic and non-enzymatic activities acting directly in pectin cleavage or indirectly disrupting non-covalent interactions. The gold standard of texture analyses is the sensorial one, however several issues make sensorial analyses inapplicable to breeding programs to select plant with improved fruit texture. Several efforts were made to achieve instrumental analyses capable of substitute humans in texture analyses. To mimic the tactile sense, a discipline studying the material response to an applied force, the rheology, is applied. The easiest instrumental measure of rheology parameters is the penetrometer test, diffused to measure the firmness, but exploitable to collect the Young’s modulus and the slope of yield stress represented respectively elasticity and fracturability. In peach, so far at least four textures were described, melting (M), stony hard (SH), non-melting (NM) and slow softening (SS). Prior to this work, no reliable objective nor fast tool were available to phenotype and select the SS trait in peach germplasm. The only reliable approach was a sensorial assessment done by a texture-trained panel, requiring repeated and time-consuming assessment. An objective, instrumental method, was set up by processing the data of a digital penetrometer test. The penetrometer itself, as reported in paragraph 2, does not support the ability to discriminate among the different texture types, as already reported in other works. In addition, this method appears to be affected by the fruit ripening season, since the early-ripening accessions tend to show faster loss of firmness, while the late-ripening exhibit a slower firmness loss. Using the data collected in our experiment, the texture dynamic (TD) model was developed from the observation of differences in the rheogram shape due to the elasticity and fracturability parameters. The TD model, that excludes the firmness effect on the fracturability and elasticity parameters, was thus developed, after testing it on 20 accessions in three years, allowing for reliable discrimination between SS and M phenotype. Differences in the… Advisors/Committee Members: supervisor: D. Bassi, L. Rossini, coordinatore: D. Bassi, BASSI, DANIELE, BASSI, DANIELE, ROSSINI, LAURA.

Subjects/Keywords: slow-softening; texture; peach; digital penetrometer; phenotyping; fruit quality; locus; Settore AGR/03 - Arboricoltura Generale e Coltivazioni Arboree; Settore AGR/07 - Genetica Agraria

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ciacciulli, A. (2018). FRUIT FLESH IN PEACH:CHARACTERIZATION OF THE 'SLOW SOFTENING' TEXTURE. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/540666

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ciacciulli, A.. “FRUIT FLESH IN PEACH:CHARACTERIZATION OF THE 'SLOW SOFTENING' TEXTURE.” 2018. Thesis, Università degli Studi di Milano. Accessed February 27, 2021. http://hdl.handle.net/2434/540666.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ciacciulli, A.. “FRUIT FLESH IN PEACH:CHARACTERIZATION OF THE 'SLOW SOFTENING' TEXTURE.” 2018. Web. 27 Feb 2021.

Vancouver:

Ciacciulli A. FRUIT FLESH IN PEACH:CHARACTERIZATION OF THE 'SLOW SOFTENING' TEXTURE. [Internet] [Thesis]. Università degli Studi di Milano; 2018. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2434/540666.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ciacciulli A. FRUIT FLESH IN PEACH:CHARACTERIZATION OF THE 'SLOW SOFTENING' TEXTURE. [Thesis]. Università degli Studi di Milano; 2018. Available from: http://hdl.handle.net/2434/540666

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

16. F. Bavo. SUBTYPE-SELECTIVE NEURONAL NICOTINIC ACETYLCHOLINE RECEPTOR AGONISTS AND ANTAGONISTS.

Degree: 2019, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/607332

► This PhD thesis focuses on two specific targets, belonging to the same receptor class of Nicotinic Acetylcholine Receptors (nAChRs): the α4β2 subtype and the α7… (more)

▼ This PhD thesis focuses on two specific targets, belonging to the same receptor class of Nicotinic Acetylcholine Receptors (nAChRs): the α4β2 subtype and the α7 subtype. This elaborate is divided in two parts. The aim of the first part is the design and synthesis of α4β2 selective partial agonists as potential smoking-cessation agents. The aim of the second part is the design and synthesis of α7 antagonists with mitocan properties as antitumoral agents. Part 1. In the first project, a series of 3-nitrophenyl ethers and 3-hydroxyphenyl ethers of (S)-N-methylprolinol bearing bulky and lipophilic substituents at the C5 were designed, synthesized and assayed as putative selective α4β2 ligands. Two of them, 5-substituted with a 6-hydroxy-1-hexynyl, had high α4β2 affinity and increased α4β2/α3β4 selectivity when compared with the correspondent unsubstituted parent compounds. In the second project, each -CH= of the unselective antagonist (S,R)-N-methyl-2-pyrrolidinyl-1,4-benzodioxane and of its epimer at the benzodioxane stereocenter, was replaced by a nitrogen. The resulting four diastereomeric pairs of pyrrolidinyl-pyridodioxanes, also designed as the product of rigidification of the flexible scaffolds of pyridyl ethers of N-methyl prolinol, were studied for their nicotinic affinity at the α4β2 and α3β4. The isosteric -CH= to N substitution was detrimental for all the compounds, with the only exception of N-Methyl-pyrrolidinyl 5-pyridodioxane, with the nitrogen at position 5. Indeed, this ligand had similar affinity to its benzodioxane parent compound, but it had high α4β2/α3β4 selectivity and it was shown to be a selective partial agonist. In the third project, the unselective antagonist (S,R)-N-methyl-2-pyrrolidinyl-1,4-benzodioxane and of its epimer at the benzodioxane stereocenter were substituted at position 5 of the benzodioxane moiety, to explore the possibility of introducing selectivity and/or partial agonist as previously done with -CH= to N replacement. Among the synthesized compounds, (S,S)-N-Methyl-pyrrolidinyl-5-amino-benzodioxane had slightly improved affinity at the α4β2 affinity and highly enhanced α4β2/α3β4 selectivity than the unsubstituted parent compound, and it was shown to be a very potent partial agonist. In the fourth project, we applied computational techniques to support the interpretation of the biological results regarding N-Methyl-pyrrolidinyl 5-substituted benzodioxanes and pyridodioxanes. From these findings, we suggested that partial agonism and α4β2/α3β4 selectivity could be achieved when the benzodioxane scaffold is appropriately substituted with an HBA/HBD system, that can displace a water molecule from a small and hydrophilic subpocket of the binding site. Part 2. Adenocarcinoma and glioblastoma cell lines express α7 and α9-α10 nAChRs, whose activation promotes tumor cells growth. On these cells, the triethylammoniumethyl ether of 4-stilbenol MG624, a known selective antagonist of α7 and α9-α10 nAChRs, has antiproliferative activity. The structural analogy of MG624 with… Advisors/Committee Members: supervisor: C. Bolchi, co-supervisor: M. Pallavicini, Coordinator: G. Aldini, supervisor: C. Gotti, supervisor: B. Frølund, supervisor: K. Harpsøe, BOLCHI, CRISTIANO, PALLAVICINI, MARCO, ALDINI, GIANCARLO.

Subjects/Keywords: nicotinic acetylcholine receptors; alpha7; alpha4beta2; alpha9; glioblastoma; nicotine addiction; nicotinic partial agonists; nicotinic antagonists; Settore CHIM/08 - Chimica Farmaceutica

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bavo, F. (2019). SUBTYPE-SELECTIVE NEURONAL NICOTINIC ACETYLCHOLINE RECEPTOR AGONISTS AND ANTAGONISTS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/607332

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bavo, F.. “SUBTYPE-SELECTIVE NEURONAL NICOTINIC ACETYLCHOLINE RECEPTOR AGONISTS AND ANTAGONISTS.” 2019. Thesis, Università degli Studi di Milano. Accessed February 27, 2021. http://hdl.handle.net/2434/607332.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bavo, F.. “SUBTYPE-SELECTIVE NEURONAL NICOTINIC ACETYLCHOLINE RECEPTOR AGONISTS AND ANTAGONISTS.” 2019. Web. 27 Feb 2021.

Vancouver:

Bavo F. SUBTYPE-SELECTIVE NEURONAL NICOTINIC ACETYLCHOLINE RECEPTOR AGONISTS AND ANTAGONISTS. [Internet] [Thesis]. Università degli Studi di Milano; 2019. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2434/607332.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bavo F. SUBTYPE-SELECTIVE NEURONAL NICOTINIC ACETYLCHOLINE RECEPTOR AGONISTS AND ANTAGONISTS. [Thesis]. Università degli Studi di Milano; 2019. Available from: http://hdl.handle.net/2434/607332

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

17. A. Tamburrini. ONE-POT SYNTHESIS OF THIO-GLYCOMIMETICS THROUGH RING OPENING REACTIONS.

Degree: 2019, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/607912

► Glycomimetics are compounds able to mimic structural and functional aspects of the corresponding natural carbohydrates. The main goal of using these mimetics, e.g. as therapeutic… (more)

▼ Glycomimetics are compounds able to mimic structural and functional aspects of the corresponding natural carbohydrates. The main goal of using these mimetics, e.g. as therapeutic agents, is the manipulation of the chemical information encoded by sugars, controlling and altering the flow of these information. Sugars, and their mimetics, are selectively recognised by certain proteins, called lectins. An example of these lectins is DC-SIGN. DC-SIGN (Dendritic Cell-Specific ICAM-3 Grabbing Non-integrin) is a tetrameric calcium dependent (C-type) lectin expressed by dermal dendritic cells (DCs) and it is known to play a crucial role in the immunological system. In particular, DC-SIGN is the primary target of Human Immunodeficiency Virus type I (HIV-1) that hijacks DCs to disseminate the infection in the human body. With the aim to develop DC-SIGN antagonists able to mimic the main natural ligand, the Bernardi’s group has developed a series of mono- and multivalent ligands that all contain a mannose unit (whose hydroxyl groups in position 3 and 4 are involved in the interaction with the calcium ion of the Carbohydrate Recognition Domain (CRD) of DC-SIGN) connected with a conformationally locked cyclohexandiol moiety (the aglycon part, that is known to exploit additional interaction with residues present in the binding site). In the course of my PhD, I have developed a straightforward synthesis for a new class of glycomimetic DC-SIGN ligands more metabolically stable, thanks to the presence of a sulfur atom in the interglycosidic position rather than the oxygen atom of the previous library of compounds. The key step of the preparation of these thio-glycomimetics is a one-pot ring opening reaction of an enantiomerically pure epoxide by an S-acetyl-α-mannose that affords the desired pseudo-thiodisaccharide as a single isomer in 77% yield after 1 hour at 60°C under micro-wave irradiation. To further improve the process, the same reaction was also performed into a flow micro-reactor at different flow rates, temperatures and residence times. Although at certain conditions a promising conversion of 69% was observed, the results were not competitive with the ones already achieved by using a MW system, which allowed also the scale up of the process. Computational studies supported by NMR analysis showed that the new thio-glycomimetic shares a very similar conformational behaviour as the corresponding O-linked pseudo-disaccharide. Furthermore, SPR inhibition assay revealed that they share also the same affinity towards DC-SIGN (IC50 value of 0.8 mM). However, a stability test, performed against an α-mannosidase enzyme, proved that the presence of the sulfur in the interglycosidic position makes the structure significantly more stable than that of the same molecule bearing instead an oxygen in that position. The low sub-millimolar activity of monovalent ligands is characteristic of oligosaccharide-protein interactions and is overcome both in Nature and in the development of biological active glycomimetics by exploiting… Advisors/Committee Members: tutor: A. Bernardi, coordinatore: E. Licandro, BERNARDI, ANNA, LICANDRO, EMANUELA.

Subjects/Keywords: Glycomimetics; DC-SIGN; one-pot reaction; carbohydrates; aziridines; Settore CHIM/06 - Chimica Organica

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tamburrini, A. (2019). ONE-POT SYNTHESIS OF THIO-GLYCOMIMETICS THROUGH RING OPENING REACTIONS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/607912

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tamburrini, A.. “ONE-POT SYNTHESIS OF THIO-GLYCOMIMETICS THROUGH RING OPENING REACTIONS.” 2019. Thesis, Università degli Studi di Milano. Accessed February 27, 2021. http://hdl.handle.net/2434/607912.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tamburrini, A.. “ONE-POT SYNTHESIS OF THIO-GLYCOMIMETICS THROUGH RING OPENING REACTIONS.” 2019. Web. 27 Feb 2021.

Vancouver:

Tamburrini A. ONE-POT SYNTHESIS OF THIO-GLYCOMIMETICS THROUGH RING OPENING REACTIONS. [Internet] [Thesis]. Università degli Studi di Milano; 2019. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2434/607912.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tamburrini A. ONE-POT SYNTHESIS OF THIO-GLYCOMIMETICS THROUGH RING OPENING REACTIONS. [Thesis]. Università degli Studi di Milano; 2019. Available from: http://hdl.handle.net/2434/607912

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

18. L. Straniero. UNRAVELING THE MOLECULAR COMPLEXITY OF PARKINSON¿S DISEASE: FROM GENETIC RISK FACTORS TO MENDELIAN CAUSATIVE GENES.

Degree: 2017, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/504504

► Parkinson’s disease (PD) is a neurodegenerative disorder characterized by the progressive death of dopaminergic neurons in the substantia nigra. PD is a complex disease caused… (more)

▼ Parkinson’s disease (PD) is a neurodegenerative disorder characterized by the progressive death of dopaminergic neurons in the substantia nigra. PD is a complex disease caused by the combination of environmental factors and genetic components. In recent years, considerable progresses have been made in the identification of the genetic determinants of PD: several genes were shown to cause rare monogenic forms of the disease. Moreover, a larger number of predisposing genetic variants have been associated with sporadic PD by genome-wide association studies. Among them, GBA seems to be the main genetic risk factor for PD. However, despite these advancements, a large fraction of the expected PD heritability is still missing. In this frame, the main aims of my PhD project were, on one hand, the study of the possible mechanisms of regulation of GBA, in particular the assessment of the role of GBAP1, its pseudogene, with the view to identify novel strategies to augment glucocerebrosidase activity and, on the other hand, the identification of novel genetic determinants for the disease by whole-exome sequencing (WES) of selected PD families. Concerning GBA we demonstrated that mir-22-3p can modulate the levels of GBA transcripts down-regulating GCase expression and that GBAP1 might work as competing endogenous RNA acting as sponge and decreasing the miRNA mediated control on GBA. We then characterized the splicing pattern of the pseudogene and we showed that GBAP1 transcripts are down-regulated by the nonsense-mediated mRNA decay mechanism. On the basis of these results, we propose the existence of an RNA-based complex regulatory network involving GBA, GBAP1 and miR-22-3p. Regarding the identification of novel PD genes, 24 PD families with a dominant or a recessive inheritance pattern were selected and whole-exome sequencing was performed on the proband of each family and on affected cousins or uncles, when available. Six of these families resulted carriers of mutations in genes already associated with parkinsonism (4 GBA, 1 LRRK2, and 1 ATP7B). Moreover, we found 2 homozygous mutations in novel candidate genes in 2 consanguineous families: a splicing mutation in a gene involved in the unfolded protein response (DNAJC12) and a missense variant in a gene coding for a lysosomal enzyme (HGSNAT). We characterized the splicing mutation and we demonstrated that this variation causes the skipping of the downstream exon and the introduction of the premature stop codon. Interestingly, we demonstrated that the silencing of the DNAJC12 gene increases the α-synuclein protein levels in SH-SY5Y neuroblastoma cells. Concerning the missense mutation, we measured the activity of the HGSNAT enzyme in the patient’s fibroblasts, which resulted below the lower limit of the normal range. We hypothesize that the HGSNAT activity is not reduced enough to cause a lysosomal disorder, but may predispose to PD. Moreover, the pathogenic role of the two novel PD genes identified in my PhD project is strongly supported by the identification, by us… Advisors/Committee Members: tutor: G. Soldà, coordinatore: M. Muzi-Falconi.

Subjects/Keywords: Settore BIO/11 - Biologia Molecolare; Settore BIO/13 - Biologia Applicata

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Straniero, L. (2017). UNRAVELING THE MOLECULAR COMPLEXITY OF PARKINSON¿S DISEASE: FROM GENETIC RISK FACTORS TO MENDELIAN CAUSATIVE GENES. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/504504

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Straniero, L.. “UNRAVELING THE MOLECULAR COMPLEXITY OF PARKINSON¿S DISEASE: FROM GENETIC RISK FACTORS TO MENDELIAN CAUSATIVE GENES.” 2017. Thesis, Università degli Studi di Milano. Accessed February 27, 2021. http://hdl.handle.net/2434/504504.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Straniero, L.. “UNRAVELING THE MOLECULAR COMPLEXITY OF PARKINSON¿S DISEASE: FROM GENETIC RISK FACTORS TO MENDELIAN CAUSATIVE GENES.” 2017. Web. 27 Feb 2021.

Vancouver:

Straniero L. UNRAVELING THE MOLECULAR COMPLEXITY OF PARKINSON¿S DISEASE: FROM GENETIC RISK FACTORS TO MENDELIAN CAUSATIVE GENES. [Internet] [Thesis]. Università degli Studi di Milano; 2017. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2434/504504.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Straniero L. UNRAVELING THE MOLECULAR COMPLEXITY OF PARKINSON¿S DISEASE: FROM GENETIC RISK FACTORS TO MENDELIAN CAUSATIVE GENES. [Thesis]. Università degli Studi di Milano; 2017. Available from: http://hdl.handle.net/2434/504504

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

19. F.I. Usheva. ESSAYS IN THE ECONOMICS OF MIGRATION.

Degree: 2017, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/534466

► The phd thesis falls within the broad topic of migrants’ integration in the host countries. The first chapter of the thesis looks at the effect… (more)

▼ The phd thesis falls within the broad topic of migrants’ integration in the host countries. The first chapter of the thesis looks at the effect of access to citizenship on saving behavior of migrant households. Naturalization of migrants is probably the last step towards full integration in the host-country society. Citizenship status gives immigrants numerous possibilities such as the right to participate in (national) elections and have political influence over which politicians are elected, what policies adopted and how resources are allocated. In addition to giving inalienable right of residence, occupational freedom and freedom of movement, it also ensures the access to social security benefits during retirement and other periods of uncertainty. The empirical strategy exploits two immigration reforms in Germany that took place in 1991 and 1999 respectively. The 1991 reform defined for the first time explicit criteria for naturalization with still restrictive residency requirement of 15 years. The 1999 reform relaxed this requirement to 8 years. Using the German Socio-Economic Panel for the period 1996 to 2012, our results show that immigrants significantly reduce their propensity to save and amount saved with each additional year after naturalization. Considering only domestic savings, however, gives us an incomplete picture on the saving behavior after naturalization. This is because migrants have the option to save in their home countries. Using data on remittances, we show that access to citizenship does not affect financial transfers sent abroad. The second paper fits within the increasing literature on the role of age at migration in the socio-economic integration of migrants. The main focus has been on education, employment and social integration, finding that the earlier a migrant arrives in the host country, the better the outcomes he/she will have later in life. These findings are attributed to living condition and surrounding environment both pre and post migration. The paper, instead, looks at juvenile criminal behavior. The criminology literature suggests that immigrants may have higher crime rates as compared to natives because of their adverse early life conditions. Longer exposure to civil war or political and ethnic prosecution in the country of origin may have long lasting effects on delinquent behavior. It may cause temporary or permanent trauma in children, making them more likely to commit an offense as juveniles or adults. Using rich Danish administrative data for the period 1980-2011, we answer two main questions: Are first generation immigrant children more crime prone relative to their native born siblings? Second, can age at migration explain the first versus second generation crime gap? Results show that first generation immigrant children are significantly more likely to be charged with or convicted of a crime by age 21 as compared to their Danish-born siblings. Looking at specific types of crime, the effect is statistically significant for property crimes only. Being born… Advisors/Committee Members: tutor: T. Frattini, FRATTINI, TOMMASO.

Subjects/Keywords: Settore SECS-P/01 - Economia Politica

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Usheva, F. (2017). ESSAYS IN THE ECONOMICS OF MIGRATION. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/534466

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Usheva, F.I.. “ESSAYS IN THE ECONOMICS OF MIGRATION.” 2017. Thesis, Università degli Studi di Milano. Accessed February 27, 2021. http://hdl.handle.net/2434/534466.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Usheva, F.I.. “ESSAYS IN THE ECONOMICS OF MIGRATION.” 2017. Web. 27 Feb 2021.

Vancouver:

Usheva F. ESSAYS IN THE ECONOMICS OF MIGRATION. [Internet] [Thesis]. Università degli Studi di Milano; 2017. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2434/534466.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Usheva F. ESSAYS IN THE ECONOMICS OF MIGRATION. [Thesis]. Università degli Studi di Milano; 2017. Available from: http://hdl.handle.net/2434/534466

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

20. B. Sciandrone. TEMPERATURE-DEPENDENT REGULATION OF THE LPXT GENE IN ESCHERICHIA COLI AND PSEUDOMONAS AERUGINOSA.

Degree: 2017, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/532118

► Bacteria respond to temperature variation through sophisticated regulatory networks that involve different macromolecules and molecular thermosensors. In particular, RNA thermometers (RNATs) are thermolabile secondary structures… (more)

▼ Bacteria respond to temperature variation through sophisticated regulatory networks that involve different macromolecules and molecular thermosensors. In particular, RNA thermometers (RNATs) are thermolabile secondary structures located within the 5’-UTR of some mRNAs that permit fast response to temperature changes. Typically, the RNATs at low temperature entrap the Translational Initiation Region (TIR) of the cognate mRNA thus inhibiting ribosome binding. When the temperature increases, the RNAT secondary structure becomes unstable and gradually shifts to an open conformation, thus allowing translation. Interestingly, in pathogenic bacteria RNATs have been found to respond to the mammal host temperature (37°C) and control the expression of virulence genes. Therefore, the identification of RNATs in pathogens could provide new information about invasion and pathogenicity strategies. RNATs are characterized by poor sequence conservation and structural variability that make the bioinformatic analysis hardly applicable to RNATs identification. We developed a new genetic tool, namely the Tet-Trap, aimed to the identification of post-transcriptionally regulated genes. We applied the Tet-Trap to find out new genes regulated by temperature in the opportunistic pathogen Pseudomonas aeruginosa. Using this system, we identified four new putative RNATs. Two of them, namely ptxS and lpxT, were validated with both in vitro and in vivo approaches. On the whole, our results strongly suggest the presence of new RNATs in the 5’-UTR of both ptxS and lpxT. The LpxT protein modifies the outer membrane of Gram negative bacteria by transferring a phosphate group from undecaprenyl-pyrophosphate to the position 1 of the Lipid A moiety of the lipopolysaccharide (LPS), generating a Lipid A diphosphate specie. One-third of the lipid A found in the E. coli outer membrane contains an unsubstituted diphosphate unit at position 1. The high proportion of this modification in Lipid A and its wide distribution among Gram-negative bacteria make relevant the clarification of its regulation and biological role. We observed that in E. coli, the lpxT gene 5’-UTR is predicted to fold into an unstable stem-loop entrapping the TIR, thus suggesting a conserved lpxT regulatory strategy in E. coli and P. aeruginosa. This observation prompted us to analyze the expression of the E. coli lpxT gene at different temperatures. We found that the E. coli lpxT gene expression is temperature-responsive. Moreover, toeprinting and reporter translational fusion experiments indicated that thermoregulation was achieved through translation modulation and that the determinants of thermoregulation were located in the lpxT 5’-UTR. Point mutations in the lpxT 5’-UTR predicted to change the stability of the stem-loop involving the TIR or enhancing the complementarity of the SD with the 16S rRNA affected thermoregulation, showing that both these elements cooperate in lpxT regulation. Overall, our results strongly suggest that in E. coli, lpxT translation may be modulated in… Advisors/Committee Members: tutor: F. Briani, BRIANI, FEDERICA.

Subjects/Keywords: Settore BIO/11 - Biologia Molecolare; Settore BIO/18 - Genetica; Settore BIO/19 - Microbiologia Generale

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sciandrone, B. (2017). TEMPERATURE-DEPENDENT REGULATION OF THE LPXT GENE IN ESCHERICHIA COLI AND PSEUDOMONAS AERUGINOSA. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/532118

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sciandrone, B.. “TEMPERATURE-DEPENDENT REGULATION OF THE LPXT GENE IN ESCHERICHIA COLI AND PSEUDOMONAS AERUGINOSA.” 2017. Thesis, Università degli Studi di Milano. Accessed February 27, 2021. http://hdl.handle.net/2434/532118.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sciandrone, B.. “TEMPERATURE-DEPENDENT REGULATION OF THE LPXT GENE IN ESCHERICHIA COLI AND PSEUDOMONAS AERUGINOSA.” 2017. Web. 27 Feb 2021.

Vancouver:

Sciandrone B. TEMPERATURE-DEPENDENT REGULATION OF THE LPXT GENE IN ESCHERICHIA COLI AND PSEUDOMONAS AERUGINOSA. [Internet] [Thesis]. Università degli Studi di Milano; 2017. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2434/532118.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sciandrone B. TEMPERATURE-DEPENDENT REGULATION OF THE LPXT GENE IN ESCHERICHIA COLI AND PSEUDOMONAS AERUGINOSA. [Thesis]. Università degli Studi di Milano; 2017. Available from: http://hdl.handle.net/2434/532118

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

21. M. Galli. YEAST HASPIN KINASE REGULATES MITOTIC CELL CYCLE EVENTS: FROM G2/M TRANSITION TO POLARISOME DISPERSION.

Degree: 2017, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/532121

► Haspin is a serine/threonine atypical kinase that phosphorylates histone H3-T3 during metaphase, promoting the recruitment of the chromosomal passenger complex (CPC) at kinetochores. Haspin depletion… (more)

▼ Haspin is a serine/threonine atypical kinase that phosphorylates histone H3-T3 during metaphase, promoting the recruitment of the chromosomal passenger complex (CPC) at kinetochores. Haspin depletion leads to cell arrest in mitosis and prevents proper chromosome positioning at the metaphase plate. Saccharomyces cerevisiae genome encodes for two haspin paralogues ALK1 and ALK2. We recently showed that these genes are essential to coordinate polarization and cell cycle progression, ensuring the correct positioning of several polarity factors following a transient mitotic delay. The aim of this project is to identify new processes where haspin kinase is involved. The first part of this work shows that Alk1 has a role at the G2/M transition in S. cerevisiae. These findings constitute the first evidence for Alk1-specific functions that are not shared by its paralogue Alk2. Our results indicate that cells lacking ALK1 are sensitive to Latrunculin A and complete nuclear division within the unbudded mother cells. These observations pointed toward a defect in the morphogenesis checkpoint. We also observed that in absence of ALK1 the Cdc28-Y19 phosphorylation signal decreases significantly during a morphogenetic stress. Exploring the underlying mechanism, we found that the decrease in phosphorylation is caused by a misregulation in Mih1 phosphatase activity in absence of Alk1. Therefore in budding yeast Alk1 modulates G2/M cell cycle switch by regulating Mih1 3 activity. The second part of this work is focused on exploring the role of Alk1 and Alk2 in polarisome dispersion. We show that the previously reported role of haspin in polarization relies on its ability to modulate Ras localization. Our observations are indicative for a mitotic role of Ras, which, by regulating Cdc24 redistribution, influences Cdc42 activation at polarized sites. These observations may help to shed light on alterations in cell polarity, which often constitute the molecular mechanism for cancer insurgence. Advisors/Committee Members: scientific tutor: M. Muzi-Falconi, MUZI FALCONI, MARCO.

Subjects/Keywords: Settore BIO/11 - Biologia Molecolare

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Galli, M. (2017). YEAST HASPIN KINASE REGULATES MITOTIC CELL CYCLE EVENTS: FROM G2/M TRANSITION TO POLARISOME DISPERSION. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/532121

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Galli, M.. “YEAST HASPIN KINASE REGULATES MITOTIC CELL CYCLE EVENTS: FROM G2/M TRANSITION TO POLARISOME DISPERSION.” 2017. Thesis, Università degli Studi di Milano. Accessed February 27, 2021. http://hdl.handle.net/2434/532121.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Galli, M.. “YEAST HASPIN KINASE REGULATES MITOTIC CELL CYCLE EVENTS: FROM G2/M TRANSITION TO POLARISOME DISPERSION.” 2017. Web. 27 Feb 2021.

Vancouver:

Galli M. YEAST HASPIN KINASE REGULATES MITOTIC CELL CYCLE EVENTS: FROM G2/M TRANSITION TO POLARISOME DISPERSION. [Internet] [Thesis]. Università degli Studi di Milano; 2017. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2434/532121.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Galli M. YEAST HASPIN KINASE REGULATES MITOTIC CELL CYCLE EVENTS: FROM G2/M TRANSITION TO POLARISOME DISPERSION. [Thesis]. Università degli Studi di Milano; 2017. Available from: http://hdl.handle.net/2434/532121

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

22. F. Asa'Ad. ALVEOLAR BONE AUGMENTATION PRIOR TO DENTAL IMPLANT PLACEMENT: VOLUME ANALYSIS & APPLICATIONS OF PRE-AUGMENTATION SOFT TISSUE EXPANSION (STE) AND REGENERATION WITH 3D PRINTED SCAFFOLDS.

Degree: 2017, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/534216

► 1.1. ASTRATTO OBIETTIVI: Lo scopo di questa revisione era di esplorare lo sviluppo degli espansori di tessuti molli, i loro diversi tipi e le loro… (more)

Subjects/Keywords: guided bone regeneration; soft tissue expanders; soft tissue expansion; soft tissue management; bone graft; biomaterials; 3D printed scaffolds; bone tissue engineering; periodontal regeneration; mandible; alveolar bone grafting; scaffold; cone-beam computed tomography; Settore MED/28 - Malattie Odontostomatologiche

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Asa'Ad, F. (2017). ALVEOLAR BONE AUGMENTATION PRIOR TO DENTAL IMPLANT PLACEMENT: VOLUME ANALYSIS & APPLICATIONS OF PRE-AUGMENTATION SOFT TISSUE EXPANSION (STE) AND REGENERATION WITH 3D PRINTED SCAFFOLDS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/534216

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Asa'Ad, F.. “ALVEOLAR BONE AUGMENTATION PRIOR TO DENTAL IMPLANT PLACEMENT: VOLUME ANALYSIS & APPLICATIONS OF PRE-AUGMENTATION SOFT TISSUE EXPANSION (STE) AND REGENERATION WITH 3D PRINTED SCAFFOLDS.” 2017. Thesis, Università degli Studi di Milano. Accessed February 27, 2021. http://hdl.handle.net/2434/534216.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Asa'Ad, F.. “ALVEOLAR BONE AUGMENTATION PRIOR TO DENTAL IMPLANT PLACEMENT: VOLUME ANALYSIS & APPLICATIONS OF PRE-AUGMENTATION SOFT TISSUE EXPANSION (STE) AND REGENERATION WITH 3D PRINTED SCAFFOLDS.” 2017. Web. 27 Feb 2021.

Vancouver:

Asa'Ad F. ALVEOLAR BONE AUGMENTATION PRIOR TO DENTAL IMPLANT PLACEMENT: VOLUME ANALYSIS & APPLICATIONS OF PRE-AUGMENTATION SOFT TISSUE EXPANSION (STE) AND REGENERATION WITH 3D PRINTED SCAFFOLDS. [Internet] [Thesis]. Università degli Studi di Milano; 2017. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2434/534216.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Asa'Ad F. ALVEOLAR BONE AUGMENTATION PRIOR TO DENTAL IMPLANT PLACEMENT: VOLUME ANALYSIS & APPLICATIONS OF PRE-AUGMENTATION SOFT TISSUE EXPANSION (STE) AND REGENERATION WITH 3D PRINTED SCAFFOLDS. [Thesis]. Università degli Studi di Milano; 2017. Available from: http://hdl.handle.net/2434/534216

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

23. R. Bulgari. STUDIES OF QUALITY AND NUTRIENT USE EFFICIENCY IN VEGETABLE CROPS GROWN UNDER DIFFERENT SUSTAINABLE CROPPING SYSTEM.

Degree: 2018, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/533673

►

Lo scopo del progetto di dottorato è stato quello di valutare gli effetti a livello fisiologico, biochimico e molecolare di prodotti biostimolanti su ortaggi da… (more)

▼

Lo scopo del progetto di dottorato è stato quello di valutare gli effetti a livello fisiologico, biochimico e molecolare di prodotti biostimolanti su ortaggi da foglia (lattuga e rucola) e di approfondire la conoscenza sulla loro modalità di azione. La prima parte del lavoro ha riguardato lo studio degli effetti di estratti acquosi di foglie (LE) e fiori (FE) di Borago officinalis L. su piante di lattuga, attraverso l’utilizzo di tecniche di fenomica, agronomia, fisiologia e biochimica. I risultati ottenuti hanno mostrato come gli estratti di borragine siano in grado di aumentare il metabolismo primario e secondario nelle piante trattate. La concentrazione di flavonoidi totali, fenoli e proteine totali, come anche l’attività in vitro della PAL e dei relativi livelli di proteine hanno mostrato un aumento a seguito dei trattamenti, in particolare dopo l’applicazione dell’estratto a base di fiori di borraggine. L’estratto di fiori si è dimostrato efficace anche nel prevenire la degradazione dei pigmenti fotosintetici durante la conservazione post raccolta di foglie di lattuga. Si può quindi affermare che gli estratti a base di borragine, in particolare l’estratto a base di fiori, sembrano possedere attività biostimolante su piante di lattuga. Gli stessi estratti sono stati applicati anche su piante di rucola, per verificare l’influenza dei trattamenti sul metabolismo del nitrato e osservare le risposte indotte a livello molecolare. È stata effettuata l’analisi dell’espressione genica dei principali geni che codificano per gli enzimi coinvolti nel metabolismo del nitrato (nitrato reduttasi DtNR, nitrito reduttasi DtNiR, glutammato sintasi DtGLU, glutammina sintetasi DtGS1, trasportatore del nitrato DtNTR). Il risultato più interessante è stato, a livello biochimico, la marcata riduzione della concentrazione di nitrato nelle foglie di rucola a seguito dei trattamenti, influenza confermata anche dall’incremento dell’attività della nitrato reduttasi in vivo. I trattamenti hanno influenzato anche l’espressione dei geni studiati, confermando che gli estratti testati hanno un ruolo nei processi fisiologici in cui tali geni sono coinvolti. Sono state anche svolte analisi mirate ad una migliore caratterizzazione gli estratti. È stata valutata l’attività ormono-simile degli estratti su mutanti di mais e, considerando le numerose proprietà attribuite alla borragine, è stata anche testata la possibile attività allelopatica degli estratti sulla germinazione di differenti specie erbacee. L’estratto a base di foglie ha mostrato un moderato effetto auxino-simile. Entrambi gli estratti di borragine sembrano possedere un effetto di inibizione sulla germinazione delle specie testate. È stata inoltre condotta un’attività in collaborazione con un’azienda multinazionale per valutare l’efficacia di alcuni biostimolanti commerciali e prototipi sulla qualità di ortaggi da foglia e sulla protezione contro stress di tipo abiotico (in particolare stress salino).

The purpose of the Ph. D. research project was to investigate the effects of…

Advisors/Committee Members: supervisor: A. Ferrante, coordinator: D. Bassi, FERRANTE, ANTONIO, BASSI, DANIELE.

Subjects/Keywords: biostimulant characterization; Borago officinalis L.; vegetables quality; nutrient use; sustainable agriculture; abiotic stress; Settore AGR/04 - Orticoltura e Floricoltura

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bulgari, R. (2018). STUDIES OF QUALITY AND NUTRIENT USE EFFICIENCY IN VEGETABLE CROPS GROWN UNDER DIFFERENT SUSTAINABLE CROPPING SYSTEM. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/533673

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bulgari, R.. “STUDIES OF QUALITY AND NUTRIENT USE EFFICIENCY IN VEGETABLE CROPS GROWN UNDER DIFFERENT SUSTAINABLE CROPPING SYSTEM.” 2018. Thesis, Università degli Studi di Milano. Accessed February 27, 2021. http://hdl.handle.net/2434/533673.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bulgari, R.. “STUDIES OF QUALITY AND NUTRIENT USE EFFICIENCY IN VEGETABLE CROPS GROWN UNDER DIFFERENT SUSTAINABLE CROPPING SYSTEM.” 2018. Web. 27 Feb 2021.

Vancouver:

Bulgari R. STUDIES OF QUALITY AND NUTRIENT USE EFFICIENCY IN VEGETABLE CROPS GROWN UNDER DIFFERENT SUSTAINABLE CROPPING SYSTEM. [Internet] [Thesis]. Università degli Studi di Milano; 2018. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2434/533673.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bulgari R. STUDIES OF QUALITY AND NUTRIENT USE EFFICIENCY IN VEGETABLE CROPS GROWN UNDER DIFFERENT SUSTAINABLE CROPPING SYSTEM. [Thesis]. Università degli Studi di Milano; 2018. Available from: http://hdl.handle.net/2434/533673

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

24. C. Giannasi. EFFECTS OF N-CONTAINING BISPHOSPHONATES ON HUMAN PRIMARY OSTEOBLASTS DERIVED FROM A HETEROGENEOUS PATIENT COHORT.

Degree: 2017, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/533647

► Bisphosphonates (BPs) are prescribed to treat a wide range of skeletal disorders, including osteoporosis, Paget disease, osteogenesis imperfecta and primary or secondary bone cancer. Their… (more)

▼ Bisphosphonates (BPs) are prescribed to treat a wide range of skeletal disorders, including osteoporosis, Paget disease, osteogenesis imperfecta and primary or secondary bone cancer. Their pharmacological action is exerted through the accumulation in areas of active bone turnover and the direct inhibition of osteoclast-mediated bone resorption. Indeed, BPs induce osteoclast apoptosis by affecting essential pathways of cell metabolism: either forming non-functional ATP analogues (non-N-containing bisphosphonates) or interfering with mevalonate pathway (N-containing bisphosphonates). However, their role in bone formation and a possible direct effect on osteoblasts (OBs) is controversial and the determination of BP concentrations at different skeletal districts is still an open issue. A serious side effect of the treatment with BPs is the osteonecrosis of jaws (BRONJ), a necrotizing and progressive lesion affecting the mandibular and/or maxillary bone. This drug-related osteonecrosis, whose etiology is not completely unravelled yet, constitutes a severe odontostomatological problem that should not be underestimated. Here, I present the direct effects of two nitrogen containing bisphosphonates, Alendronate (AL) and Zoledronate (ZL), on primary human osteoblast viability, expression and secretion of ECM proteins, functional mediators and bone biomarkers. Osteoblasts, isolated from patients who underwent total hip replacement surgery at IRCCS Galeazzi Orthopaedic Institute, were incubated with several concentrations of AL and ZL (range from 10^-15 to 10^-5M). We never observed any peculiar effect on osteoblast vitality and proliferation, except a significant inhibition induced by both drugs at 10^-5M, indicating an in vitro cytotoxicity. ALP activity was minimally enhanced at day 14 by low BP doses, while higher concentrations slightly inhibited it. We then investigated the modulation of ECM protein expression after exposure to low, intermediate and high BP concentrations (10^-13, 10^-10 and 10^-7M): Osteonectin (SPARC) was increased at day 7 by both compounds, while type I Collagen and Osteopontin (OPN) were clearly down-modulated only by AL. The secretion of bone biomarkers and cytokines was also analysed in supernatants of cultured osteoblasts at day 3. No overall effect was observed on Osteoprotegerin (OPG), DKK1 and IL-6. The levels of released TNFα, Sclerostin (SOST) and IL-1β, all inhibitors of osteoblast differentiation, were slightly enhanced by both drugs, independently on their concentrations, while OPN was mildly reduced only by 10^-7M BPs. Interestingly, DKK-1, IL-6 and Osteocalcin (OC) secretion was increased in all tested conditions by cells derived from donors <50 years old, suggesting that this subpopulation might respond more rapidly to the treatments. In conclusion, my results indicate that AL and ZL in concentrations below µM do not overall impair osteoblast viability, nor most of the analysed biomarkers. However, since SPARC expression, SOST, TNFα and IL-1β secretion are increased, we believe that… Advisors/Committee Members: tutor: A. T. Brini, head of phd program: M. Del Fabbro, BRINI, ANNA TERESA, DEL FABBRO, MASSIMO.

Subjects/Keywords: bisphosphonates; BRONJ; primary human osteoblasts; Settore BIO/14 - Farmacologia; Settore BIO/13 - Biologia Applicata

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Giannasi, C. (2017). EFFECTS OF N-CONTAINING BISPHOSPHONATES ON HUMAN PRIMARY OSTEOBLASTS DERIVED FROM A HETEROGENEOUS PATIENT COHORT. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/533647

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Giannasi, C.. “EFFECTS OF N-CONTAINING BISPHOSPHONATES ON HUMAN PRIMARY OSTEOBLASTS DERIVED FROM A HETEROGENEOUS PATIENT COHORT.” 2017. Thesis, Università degli Studi di Milano. Accessed February 27, 2021. http://hdl.handle.net/2434/533647.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Giannasi, C.. “EFFECTS OF N-CONTAINING BISPHOSPHONATES ON HUMAN PRIMARY OSTEOBLASTS DERIVED FROM A HETEROGENEOUS PATIENT COHORT.” 2017. Web. 27 Feb 2021.

Vancouver:

Giannasi C. EFFECTS OF N-CONTAINING BISPHOSPHONATES ON HUMAN PRIMARY OSTEOBLASTS DERIVED FROM A HETEROGENEOUS PATIENT COHORT. [Internet] [Thesis]. Università degli Studi di Milano; 2017. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2434/533647.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Giannasi C. EFFECTS OF N-CONTAINING BISPHOSPHONATES ON HUMAN PRIMARY OSTEOBLASTS DERIVED FROM A HETEROGENEOUS PATIENT COHORT. [Thesis]. Università degli Studi di Milano; 2017. Available from: http://hdl.handle.net/2434/533647

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

25. A. Valmadre. A NEW PROCEDURE TO ISOLATE BRAIN MITOCHONDRIA FROM HUMAN CORTEX AND ITS APPLICATION FOR LIPID ANALYSIS IN PHYSIOLOGICAL AGING.

Degree: 2018, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/534411

► Many studies have revealed the importance of mitochondria as cellular organelles decisively involved in the onset or progression of neurodegenerative diseases, whose main risk factor… (more)

Subjects/Keywords: mitochondria; aging; lipid; human; brain; Settore MED/26 - Neurologia

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Valmadre, A. (2018). A NEW PROCEDURE TO ISOLATE BRAIN MITOCHONDRIA FROM HUMAN CORTEX AND ITS APPLICATION FOR LIPID ANALYSIS IN PHYSIOLOGICAL AGING. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/534411

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Valmadre, A.. “A NEW PROCEDURE TO ISOLATE BRAIN MITOCHONDRIA FROM HUMAN CORTEX AND ITS APPLICATION FOR LIPID ANALYSIS IN PHYSIOLOGICAL AGING.” 2018. Thesis, Università degli Studi di Milano. Accessed February 27, 2021. http://hdl.handle.net/2434/534411.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Valmadre, A.. “A NEW PROCEDURE TO ISOLATE BRAIN MITOCHONDRIA FROM HUMAN CORTEX AND ITS APPLICATION FOR LIPID ANALYSIS IN PHYSIOLOGICAL AGING.” 2018. Web. 27 Feb 2021.

Vancouver:

Valmadre A. A NEW PROCEDURE TO ISOLATE BRAIN MITOCHONDRIA FROM HUMAN CORTEX AND ITS APPLICATION FOR LIPID ANALYSIS IN PHYSIOLOGICAL AGING. [Internet] [Thesis]. Università degli Studi di Milano; 2018. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2434/534411.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Valmadre A. A NEW PROCEDURE TO ISOLATE BRAIN MITOCHONDRIA FROM HUMAN CORTEX AND ITS APPLICATION FOR LIPID ANALYSIS IN PHYSIOLOGICAL AGING. [Thesis]. Università degli Studi di Milano; 2018. Available from: http://hdl.handle.net/2434/534411

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

26. G. Fantoni. IL RUOLO DEI SOCI E DEGLI AMMINISTRATORI DI SOCIETA' DI CAPITALI IN CRISI.

Degree: 2018, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/576761

► At a time of global economic and financial crisis in which the issue about distressed enterprises has become increasingly important, we analyse in particular the… (more)

▼ At a time of global economic and financial crisis in which the issue about distressed enterprises has become increasingly important, we analyse in particular the role of the main actors involved in distressed capital companies: the shareholders and the directors, with particular reference to the creditors as well. However, since the “crisis” is a multifaceted concept, we make several distinctions, attempting to point out that not every form of crisis is able to damage firstly the creditors. Depending on the form of crisis and its severity, we show how the directors’ duties change regarding the interests that have to be first and foremost protected, as well as their duties change (or must change) in order to prevent the deepening of the crisis, as well as change the role of both shareholders and creditors in managing the crisis. On the one hand, we point out the importance of the role of the directors (and of the auditors as well) to prevent the crisis in all its forms, in light of the duty to act on an informed (and “adequate”) basis; and subsequently to put in place a prompt intervention before the company faces serious troubles in order to implement an early corporate restructuring to preserve the economically sustainable parts of the business. In the last chapter, we analyse the proceeding of the arrangement with creditors, in relation to which the legal provisions of the bankruptcy law are completely different from those of the civil corporate law, in particular as regards the management objectives as well as the competences to take decisions affecting the corporate structure. In such a situation where creditors are at risk of suffering the default of the company, we consider in particular, including on the basis of a comparative analysis with different (especially european) legal systems, if it is reasonable to assume that the directors have the “shifting duty” to find a better solution in order to protect, besides the creditors, the shareholders as well in the event that the company is potentially economically sustainable; taking account that following the introduction of the competing proposals and the “arrangement-with-creditors expropriation” ex art. 163, par. 4, l.f. (as well as the intended introduction of the possibility for third parties to submit the proposal for the admission to the proceeding of the arrangement with creditors), the shareholders, who are most likely to be affected by the legal effects – even serious – of a competition procedure, are completely sidelined from any form of participation both from the decision about the an and quomodo of the procedure and, subsequently, from conducting the procedure by evaluating the arrangement-with-creditors proposal, because of the shareholders’ exclusion from the vote, on a par with the creditors, for the approval of the proposal; for which reason we consider the possibility to assume the necessity for some forms of legal protection for the shareholders throughout the procedure of the arrangement with creditors. Advisors/Committee Members: relatore: G. Ferri, referente: R. Sacchi, SACCHI, ROBERTO.

Subjects/Keywords: Settore IUS/04 - Diritto Commerciale

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fantoni, G. (2018). IL RUOLO DEI SOCI E DEGLI AMMINISTRATORI DI SOCIETA' DI CAPITALI IN CRISI. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/576761

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fantoni, G.. “IL RUOLO DEI SOCI E DEGLI AMMINISTRATORI DI SOCIETA' DI CAPITALI IN CRISI.” 2018. Thesis, Università degli Studi di Milano. Accessed February 27, 2021. http://hdl.handle.net/2434/576761.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fantoni, G.. “IL RUOLO DEI SOCI E DEGLI AMMINISTRATORI DI SOCIETA' DI CAPITALI IN CRISI.” 2018. Web. 27 Feb 2021.

Vancouver:

Fantoni G. IL RUOLO DEI SOCI E DEGLI AMMINISTRATORI DI SOCIETA' DI CAPITALI IN CRISI. [Internet] [Thesis]. Università degli Studi di Milano; 2018. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2434/576761.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fantoni G. IL RUOLO DEI SOCI E DEGLI AMMINISTRATORI DI SOCIETA' DI CAPITALI IN CRISI. [Thesis]. Università degli Studi di Milano; 2018. Available from: http://hdl.handle.net/2434/576761

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

27. G. DELLA SCALA. STREPTOCOCCUS THERMOPHILUS UREASE ACTIVITY: PHYSIOLOGICAL ROLE AND TECHNOLOGICAL RELEVANCE IN DAIRY AND NON-DAIRY APPLICATIONS.

Degree: 2018, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/604396

► The aim of this Ph.D. work was to investigate the physiological role and the technological relevance of the urease activity of the dairy bacterium Streptococcus… (more)

▼ The aim of this Ph.D. work was to investigate the physiological role and the technological relevance of the urease activity of the dairy bacterium Streptococcus thermophilus. It has been achieved a deeper comprehension of this peculiar enzymatic activity following different approaches. A milk-based medium that allows the discrimination between urease-positive S. thermophilus strains and urease-negative ones based on the colonies morphology had been developed; it was used as screening method looking for urease-defective mutants after UV mutagenesis of urease-positive strains of industrial interest. Moreover, a cytofluorimetric protocol for the evaluation of urease activity of various samples containing S. thermophilus was developed: the proposed applications are related to the evaluation of the urease activity of starter culture biomasses of S. thermophilus and to the enumeration of the S. thermophilus population in probiotic products containing this species, among others. We propose that the cytofluorimetric method should be seen as an innovative tool to put besides the standard ones to evaluate the quality of the products previously mentioned. The investigation of the physiological role of urease of S. thermophilus cells growing in milk highlighted that in presence of this enzymatic activity the overall metabolism of the species is boosted. Moreover, the cooperative role of urease, previously described for the yogurt consortium, has been extended also to the cooperation between a urease-positive S. thermophilus strain and a urease-negative one, supporting the proposal of the urease activity as an altruistic cooperative trait, which is costly for urease-positive species but provides a local benefit to the urease-negative species sharing the same environment, which can take advantage of the release of ammonia. At industrial level, urease activity is still considered more for its detrimental effects than for the positive effects exerted on S. thermophilus: we proposed different strategies to overcome this industrial problem. Firstly, we produced mutants of urease-positive strains of industrial interest, but they showed lower acidification rate compared to their wild types. So, we proposed to modify the production process of the biomasses with the aim of obtaining cells which carry a lower urease activity compared to those currently produced. In conclusion, the present work gives new insight in the comprehension of the urease activity of the dairy bacterium S. thermophilus, on how it can be exploited to improve the acidification performances of the strains and how it cannot, so far, be controlled during the milk acidification processes. Advisors/Committee Members: tutor: D. Mora, coordinatore: F. Bonomi, MORA, DIEGO, BONOMI, FRANCESCO.

Subjects/Keywords: Streptococcus thermophilus; urease activity; urease-defective mutants; dairy industries; Settore AGR/16 - Microbiologia Agraria

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

SCALA, G. D. (2018). STREPTOCOCCUS THERMOPHILUS UREASE ACTIVITY: PHYSIOLOGICAL ROLE AND TECHNOLOGICAL RELEVANCE IN DAIRY AND NON-DAIRY APPLICATIONS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/604396

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

SCALA, G. DELLA. “STREPTOCOCCUS THERMOPHILUS UREASE ACTIVITY: PHYSIOLOGICAL ROLE AND TECHNOLOGICAL RELEVANCE IN DAIRY AND NON-DAIRY APPLICATIONS.” 2018. Thesis, Università degli Studi di Milano. Accessed February 27, 2021. http://hdl.handle.net/2434/604396.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

SCALA, G. DELLA. “STREPTOCOCCUS THERMOPHILUS UREASE ACTIVITY: PHYSIOLOGICAL ROLE AND TECHNOLOGICAL RELEVANCE IN DAIRY AND NON-DAIRY APPLICATIONS.” 2018. Web. 27 Feb 2021.

Vancouver:

SCALA GD. STREPTOCOCCUS THERMOPHILUS UREASE ACTIVITY: PHYSIOLOGICAL ROLE AND TECHNOLOGICAL RELEVANCE IN DAIRY AND NON-DAIRY APPLICATIONS. [Internet] [Thesis]. Università degli Studi di Milano; 2018. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2434/604396.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

SCALA GD. STREPTOCOCCUS THERMOPHILUS UREASE ACTIVITY: PHYSIOLOGICAL ROLE AND TECHNOLOGICAL RELEVANCE IN DAIRY AND NON-DAIRY APPLICATIONS. [Thesis]. Università degli Studi di Milano; 2018. Available from: http://hdl.handle.net/2434/604396

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

28. S. Sala. QUPLAS: TOWARDS ANTIMATTER INTERFEROMETRY.

Degree: 2018, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/596728

► Matter-wave interference has been observed for a wide range of objects: from electrons to neutrons and complex molecules. On the other hand, a double-slit-like experiment… (more)

Subjects/Keywords: matter-waves; interferometry; positrons; antimatter; Settore FIS/01 - Fisica Sperimentale

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sala, S. (2018). QUPLAS: TOWARDS ANTIMATTER INTERFEROMETRY. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/596728

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sala, S.. “QUPLAS: TOWARDS ANTIMATTER INTERFEROMETRY.” 2018. Thesis, Università degli Studi di Milano. Accessed February 27, 2021. http://hdl.handle.net/2434/596728.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sala, S.. “QUPLAS: TOWARDS ANTIMATTER INTERFEROMETRY.” 2018. Web. 27 Feb 2021.

Vancouver:

Sala S. QUPLAS: TOWARDS ANTIMATTER INTERFEROMETRY. [Internet] [Thesis]. Università degli Studi di Milano; 2018. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2434/596728.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sala S. QUPLAS: TOWARDS ANTIMATTER INTERFEROMETRY. [Thesis]. Università degli Studi di Milano; 2018. Available from: http://hdl.handle.net/2434/596728

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

29. A. Formozov. SEARCH FOR NON-STANDARD NEUTRINO INTERACTIONS WITH LARGE-VOLUME LIQUID SCINTILLATOR DETECTORS.

Degree: 2019, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/646205

► Even though electroweak part of the Standard Model passed decades of testing and precision measurements, there is still a space for the presence of new… (more)

▼ Even though electroweak part of the Standard Model passed decades of testing and precision measurements, there is still a space for the presence of new physics. In particular, the neutrino sector in the last years has been reaching the level of precision oscillometry. However, the current data are not able to exclude many possible extended scenarios, in which new interactions comparable with the standard ones are still possible. This work is dedicated to beyond-Standard-Model interactions of neutrino and electron called Non-Standard Interactions or shortly NSI. Analogously to standard electroweak interactions, NSI could be charged current (CC) and neutral current (NC) type. Usually, in analyses of experiments, NC and CC NSI are considered separately, and I follow this strategy in the course of this work. The global search for NSI involves almost all experimental approaches of neutrino physics and goes far beyond the scope of this work. Here, instead, I look at the problem through the prism of the large-scale liquid scintillator experiments and confine the investigation to the search for NC NSI with solar neutrinos. In addition, I review CC NSI with reactor neutrinos at medium baseline. As a contribution into upcoming JUNO experiment, I investigate liquid scintillator energy response and radiopurity, which are essential for the successful realization of solar and reactor neutrino physics programs and, therefore, for NC and CC NSI search. I analyze in detail the non-linearity and energy resolution of liquid scintillator. I develop an experimental Compton coincidence technique with High Pure Germanium gamma spectrometer, provide a conservative measurement of the Birks’ ionization quenching constant kB and discuss problems related to its measurement. Throughout the study, I also formulate the optimal characteristics of the experimental apparatus for this improvement. The ultimate goal of this investigation is separating intrinsic energy resolution, which was never yet robustly measured for liquid organic scintillators. The intrinsic energy resolution may have an impact on the energy response of the new generation large liquid scintillator detectors such as JUNO and have to be carefully investigated. Applying single photon counting technique, I perform a calibration of the PMT charge scale and set apart statistical term from the total liquid scintillator energy resolution. Remaining term significantly differs from zero, indicating the presence of additional contribution associated with the intrinsic energy resolution. As an outlook, the precise estimation of the light collection should be conducted in order to ensure that its contribution is not significant to mimic the observed intrinsic resolution effect. The analysis of the radiopurity of the liquid scintillator showed that it is close to the one demanded for reactor neutrino program and CC-NSI search. Still, an improvement of purification procedure is necessary to fulfill solar neutrino program requirements for NC NSI search. Finally, I place the limits to NC… Advisors/Committee Members: coordinator: M. Paris, co-supervisors (Lomonosov Moscow State University): A.G. Olshevskiy, Ph.D. O. Y. Smirnov, MIRAMONTI, LINO, MIRAMONTI, LINO, PARIS, MATTEO.

Subjects/Keywords: solar neutrinos; Borexino; neutrino oscillations; Non-Standard neutrino Interactions; physics beyond the Standard Model; Weinberg angle; liquid scintillator; non-linearity; intrinsic energy resolution; radiopurity; Settore FIS/04 - Fisica Nucleare e Subnucleare

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Formozov, A. (2019). SEARCH FOR NON-STANDARD NEUTRINO INTERACTIONS WITH LARGE-VOLUME LIQUID SCINTILLATOR DETECTORS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/646205

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Formozov, A.. “SEARCH FOR NON-STANDARD NEUTRINO INTERACTIONS WITH LARGE-VOLUME LIQUID SCINTILLATOR DETECTORS.” 2019. Thesis, Università degli Studi di Milano. Accessed February 27, 2021. http://hdl.handle.net/2434/646205.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Formozov, A.. “SEARCH FOR NON-STANDARD NEUTRINO INTERACTIONS WITH LARGE-VOLUME LIQUID SCINTILLATOR DETECTORS.” 2019. Web. 27 Feb 2021.

Vancouver:

Formozov A. SEARCH FOR NON-STANDARD NEUTRINO INTERACTIONS WITH LARGE-VOLUME LIQUID SCINTILLATOR DETECTORS. [Internet] [Thesis]. Università degli Studi di Milano; 2019. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2434/646205.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Formozov A. SEARCH FOR NON-STANDARD NEUTRINO INTERACTIONS WITH LARGE-VOLUME LIQUID SCINTILLATOR DETECTORS. [Thesis]. Università degli Studi di Milano; 2019. Available from: http://hdl.handle.net/2434/646205

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

30. S. Marchiano'. CLINICAL AND EXPERIMENTAL EVIDENCES OF DIRECT VASCULAR EFFECT OF PROPROTEIN CONVERTASE SUBTILISIN/KEXIN TYPE 9.

Degree: 2018, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/605175

► Proprotein Convertase Subtilisin Kexin type 9 (PCSK9), together with LDLR and APOB genes, had been identified as the third gene associated with Familial Hypercholesterolemia (FH).… (more)

▼ Proprotein Convertase Subtilisin Kexin type 9 (PCSK9), together with LDLR and APOB genes, had been identified as the third gene associated with Familial Hypercholesterolemia (FH). Secreted PCSK9 in fact, targets the hepatic LDL receptor (LDLR) for degradation thus preventing its recycling on the cell surface. The decreased expression of LDLR determines an increase in the circulating LDL particles, leading to increased cholesterol levels. Secreted PCSK9 is mainly derived from the liver, but it is also expressed in other tissues such as the brain, the kidney, the pancreas as well as the cells that composed the arterial wall. It is therefore possible that it could exert multiple paracrine effects. Our research group found that PCSK9 is expressed and secreted by smooth muscles cells (SMCs) which constitute the atherosclerotic plaque. The aim of my project was to determine the paracrine role of PCSK9 on the neointima formation through preclinical and clinical approaches. The immunohistochemical analysis of Pcsk9-/- and Pcsk9+/+ mice revealed that, after vascular manipulation, the PCSK9-null mice were protected from the formation of neointima with lower intima area (28100 ± 4901 µm2 and 14350 ± 2990 µm2 for Pcsk9+/+ and Pcsk9-/- mice respectively, p<0.05), associated with decreased intima/media ratio of 1.48 ± 0.34 and 0.60 ± 0.18 for Pcsk9+/+ and Pcsk9-/- mice respectively, (p<0.05). The in vitro studies on isolated SMCs from Pcsk9-/- and Pcsk9+/+ mice showed that the absence of PCSK9 induced a more contractile phenotype, associated with a reduced proliferation rate (doubling time were 57.3 ± 2.1h and 106.3 ± 4.5h, respectively [p<0.001]). The response to the chemotactic agent PDGF-BB (Platelet-derived growth factor), measured with Boyden’s chamber assay, was also impaired in the absence of PCSK9. These were rescued after the reconstitution of PCSK9 in the Pcsk9-/- cell line, which led to a more synthetic phenotype associated with a doubling time of 32.2± 3.1h and 41.2 ± 1.9h [p< 0.001], for Pcsk9-/- and Pcsk9-/-REC SMCs, respectively. The difference in proliferation between Pcsk9-/- and Pcsk9-/-REC SMCs was maintained also after the incubation with 40µM of simvastatin, suggesting that PCSK9 could improve SMCs proliferation through mechanism independently from cholesterol levels. The cell cycle analyses of the Pcsk9-/- and Pcsk9-/-REC SMCs showed a decreased activation of p21 and p27, associated with an increased expression of cyclin E and cyclin D1; presumably due to a different activation of the PDGF receptor pathway mediated by LRP1. Finally, the observational study, carried out in collaboration with the Brisighella Heart Study research group, demonstrated that serum levels of PCSK9, together with aging, is positively correlated to the pulse wave velocity. This is an indirect parameter used to evaluate the arterial stiffness and hence the presence of atherosclerotic plaques. Taken together, these results demonstrated that aside from its function on in regulating cholesterol homeostasis, PCSK9 plays a direct… Advisors/Committee Members: supervisor: A. Corsini, coordinatore: A. L. Catapano, CORSINI, ALBERTO, CATAPANO, ALBERICO LUIGI.

Subjects/Keywords: PCSK9; atherosclerosis; smooth muscle cells; arterial stiffness; Settore BIO/14 - Farmacologia

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Marchiano', S. (2018). CLINICAL AND EXPERIMENTAL EVIDENCES OF DIRECT VASCULAR EFFECT OF PROPROTEIN CONVERTASE SUBTILISIN/KEXIN TYPE 9. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/605175

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Marchiano', S.. “CLINICAL AND EXPERIMENTAL EVIDENCES OF DIRECT VASCULAR EFFECT OF PROPROTEIN CONVERTASE SUBTILISIN/KEXIN TYPE 9.” 2018. Thesis, Università degli Studi di Milano. Accessed February 27, 2021. http://hdl.handle.net/2434/605175.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Marchiano', S.. “CLINICAL AND EXPERIMENTAL EVIDENCES OF DIRECT VASCULAR EFFECT OF PROPROTEIN CONVERTASE SUBTILISIN/KEXIN TYPE 9.” 2018. Web. 27 Feb 2021.

Vancouver:

Marchiano' S. CLINICAL AND EXPERIMENTAL EVIDENCES OF DIRECT VASCULAR EFFECT OF PROPROTEIN CONVERTASE SUBTILISIN/KEXIN TYPE 9. [Internet] [Thesis]. Università degli Studi di Milano; 2018. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2434/605175.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Marchiano' S. CLINICAL AND EXPERIMENTAL EVIDENCES OF DIRECT VASCULAR EFFECT OF PROPROTEIN CONVERTASE SUBTILISIN/KEXIN TYPE 9. [Thesis]. Università degli Studi di Milano; 2018. Available from: http://hdl.handle.net/2434/605175

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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