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1. Wagner, Gregory Randall. Identification and characterization of altered mitochondrial protein acetylation in Friedreich's ataxia cardiomyopathy.

Degree: 2011, Hindawi Publishing Corporation and Oxford Journals and SAGE Journals

Indiana University-Purdue University Indianapolis (IUPUI)

Friedreich’s Ataxia (FRDA) is a rare and poorly understood autosomal recessive disease caused by a pathological deficiency of the mitochondrial protein frataxin. Patients suffer neurodegeneration, ataxia, diabetes, and heart failure. In an effort to understand the mechanisms of heart failure in FRDA, we investigated the role of the protein modification acetylation, which is highly abundant on mitochondrial proteins and has been implicated in regulating intermediary metabolism. Using mouse models of FRDA, we found that cardiac frataxin deficiency causes progressive hyperacetylation of mitochondrial proteins which is correlated with loss of respiratory chain subunits and an altered mitochondrial redox state. Mitochondrial protein hyperacetylation could be reversed by the mitochondria-localized deacetylase SIRT3 in vitro, suggesting a defect in endogenous SIRT3 activity. Consistently, frataxin-deficient cardiac mitochondria showed significantly decreased rates of fatty acid oxidation and complete oxidation to carbon dioxide. However, the degree of protein hyperacetylation in FRDA could not be fully explained by SIRT3 loss. Our data suggested that intermediary metabolites and perhaps acetyl-CoA, which is required for protein acetylation, are accumulating in frataxin-deficient mitochondria. Upon testing the hypothesis that mitochondrial protein acetylation is non-enzymatic, we found that the minimal chemical conditions of the mitochondrial matrix are sufficient to cause widespread non-enzymatic protein acetylation in vitro. These data suggest that mitochondrial protein hyperacetylation in FRDA cardiomyopathy mediates progressive post-translational suppression of mitochondrial oxidative pathways which is caused by a combination of SIRT3 deficiency and, likely, an accumulation of unoxidized acetyl-CoA capable of initiating non-enzymatic protein acetylation. These findings provide novel insight into the mechanisms underlying a poorly understood and fatal cardiomyopathy and highlight a fundamental biochemical mechanism that had been previously overlooked in biological systems.

Advisors/Committee Members: Payne, R. Mark, Dong, X. Charlie, Herbert, Brittney-Shea, Shou, Weinian.

Subjects/Keywords: Friedreich's ataxia  – Research  – Methodology  – Analysis; Mitochondrial pathology  – Research  – Methodology; Acetylation; Myocardium  – Diseases; Nervous system  – Degeneration; Heart failure; Metabolism  – Regulation; Histone deacetylase  – Research; Heart  – Pathophysiology; Proteomics; Mitochondrial membranes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Wagner, G. R. (2011). Identification and characterization of altered mitochondrial protein acetylation in Friedreich's ataxia cardiomyopathy. (Thesis). Hindawi Publishing Corporation and Oxford Journals and SAGE Journals. Retrieved from http://hdl.handle.net/1805/4209

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Wagner, Gregory Randall. “Identification and characterization of altered mitochondrial protein acetylation in Friedreich's ataxia cardiomyopathy.” 2011. Thesis, Hindawi Publishing Corporation and Oxford Journals and SAGE Journals. Accessed August 06, 2020. http://hdl.handle.net/1805/4209.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Wagner, Gregory Randall. “Identification and characterization of altered mitochondrial protein acetylation in Friedreich's ataxia cardiomyopathy.” 2011. Web. 06 Aug 2020.

Vancouver:

Wagner GR. Identification and characterization of altered mitochondrial protein acetylation in Friedreich's ataxia cardiomyopathy. [Internet] [Thesis]. Hindawi Publishing Corporation and Oxford Journals and SAGE Journals; 2011. [cited 2020 Aug 06]. Available from: http://hdl.handle.net/1805/4209.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wagner GR. Identification and characterization of altered mitochondrial protein acetylation in Friedreich's ataxia cardiomyopathy. [Thesis]. Hindawi Publishing Corporation and Oxford Journals and SAGE Journals; 2011. Available from: http://hdl.handle.net/1805/4209

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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