Advanced search options

Advanced Search Options 🞨

Browse by author name (“Author name starts with…”).

Find ETDs with:

in
/  
in
/  
in
/  
in

Written in Published in Earliest date Latest date

Sorted by

Results per page:

You searched for id:"oai:scholarworks.gsu.edu:chemistry_theses-1135". One record found.

Search Limiters

Last 2 Years | English Only

No search limiters apply to these results.

▼ Search Limiters


Georgia State University

1. Liu, Shuo. Synthesis and Crystallization Study of the 5-Se-Thymidine DNAs and the Complexes with Small Molecules.

Degree: MS, Chemistry, 2019, Georgia State University

There are two major challenges in nucleic acid X-ray crystallography: crystallization and phasing determination. With the introduction of selenium modification, the phasing problem can be solved rationally, while the crystallization problem can be solved partially. By taking advantage of the selenium modification, herein we report the synthesis of the Se-DNAs and the thermostability and crystallization study of the DNA-small molecule complexes. 5-Se-thymidine was successfully synthesized and incorporated into DNA oligonucleotides via solid-phase synthesis in order to facilitate the crystallization and phasing. The UV-melting study reveals that the selenium modification has caused no significate perturbation to the DNA stability. The small molecules (DB2429 and DB2457) were co-crystalized with Se-DNAs and the Se-DNA crystals were obtained, which are better than the native ones. In addition, our research suggested that the DNA oligonucleotides with the 5’-overhang form better crystals than those with the 3’-overhang or non-overhang. Our X-ray diffraction study is in progress. Advisors/Committee Members: Zhen Huang.

Subjects/Keywords: Nucleic acid; Selenium derivatization; Small molecule; Crystallization; X-ray crystallography

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Liu, S. (2019). Synthesis and Crystallization Study of the 5-Se-Thymidine DNAs and the Complexes with Small Molecules. (Thesis). Georgia State University. Retrieved from https://scholarworks.gsu.edu/chemistry_theses/130

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Liu, Shuo. “Synthesis and Crystallization Study of the 5-Se-Thymidine DNAs and the Complexes with Small Molecules.” 2019. Thesis, Georgia State University. Accessed August 24, 2019. https://scholarworks.gsu.edu/chemistry_theses/130.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Liu, Shuo. “Synthesis and Crystallization Study of the 5-Se-Thymidine DNAs and the Complexes with Small Molecules.” 2019. Web. 24 Aug 2019.

Vancouver:

Liu S. Synthesis and Crystallization Study of the 5-Se-Thymidine DNAs and the Complexes with Small Molecules. [Internet] [Thesis]. Georgia State University; 2019. [cited 2019 Aug 24]. Available from: https://scholarworks.gsu.edu/chemistry_theses/130.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Liu S. Synthesis and Crystallization Study of the 5-Se-Thymidine DNAs and the Complexes with Small Molecules. [Thesis]. Georgia State University; 2019. Available from: https://scholarworks.gsu.edu/chemistry_theses/130

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.