University of Miami
Activatable Fluorophores Combined with Amphiphilic Polymer Carriers for Bioimaging.
Degree: PhD, Chemistry (Arts and Sciences), 2018, University of Miami
Self-assembling amphiphilic macromolecular nanoparticle, with a hydrophobic inner core and hydrophilic shell, can capsulate organic dyes inside. Thus, as a result, could be a valuable delivery vehicle for biology applications. We have developed a family of polymers, as supramolecular hosts, with decyl and polyethylene glycol side chains, exhibiting different hydrophobic/hydrophilic ratio, polyethylene glycol length or molecular weight. The polymers assemble into particles in neutral buffer and capture hydrophobic BODIPY in their interior. The relationship between critical concentration, dye loading property, hydrodynamic diameter and polymer structures were investigated. These provided a guideline to design self-assembling supramolecular hosts for the encapsulation of fluorescent guests and can lead to ideal delivery vehicles for the imaging probes to target locations in biosystems. In additional, a halochromic coumarin-oxazine fluorophore was connected to the side chains of an amphiphilic polymer covalently, with folate ligands as targeting agents to MCF-7 cancer cells. The pre-fluorophores did not produce any detectable fluorescence at neutral pH, but are converted into fluorophores with red emission in acidic environment. Thus, this design offers the opportunity to highlight cancer cells selectively with high brightness and optimal contrast.
We also designed a photoactivatable BODIPY with two oxazines attached. As a result, the oxazine heterocycles can be disconnected stepwise upon UV irradiation and each product exhibit different emission bands. When relative amounts of chromophores are placed in a polystyrene bead matrix, the ratio of three component can be modulated by dose of activating photons. This ratiometric strategy can be applied to write barcode inside biosystems: different regions of a same animal can be labeled with different barcodes to allow the monitoring of the spatiotemporal tracking.
Advisors/Committee Members: Françisco M. Raymo, Roger M. Leblanc, James N. Wilson, James D. Baker.
Subjects/Keywords: Fluorescence; Molecular Switch; Amphiphilic Polymer; Bioimaging; BODIPY; Probe
to Zotero / EndNote / Reference
APA (6th Edition):
Tang, S. (2018). Activatable Fluorophores Combined with Amphiphilic Polymer Carriers for Bioimaging. (Doctoral Dissertation). University of Miami. Retrieved from https://scholarlyrepository.miami.edu/oa_dissertations/2022
Chicago Manual of Style (16th Edition):
Tang, Sicheng. “Activatable Fluorophores Combined with Amphiphilic Polymer Carriers for Bioimaging.” 2018. Doctoral Dissertation, University of Miami. Accessed July 20, 2018.
MLA Handbook (7th Edition):
Tang, Sicheng. “Activatable Fluorophores Combined with Amphiphilic Polymer Carriers for Bioimaging.” 2018. Web. 20 Jul 2018.
Tang S. Activatable Fluorophores Combined with Amphiphilic Polymer Carriers for Bioimaging. [Internet] [Doctoral dissertation]. University of Miami; 2018. [cited 2018 Jul 20].
Available from: https://scholarlyrepository.miami.edu/oa_dissertations/2022.
Council of Science Editors:
Tang S. Activatable Fluorophores Combined with Amphiphilic Polymer Carriers for Bioimaging. [Doctoral Dissertation]. University of Miami; 2018. Available from: https://scholarlyrepository.miami.edu/oa_dissertations/2022