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You searched for id:"oai:minds.wisconsin.edu:1793/78721". One record found.

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University of Wisconsin – Oshkosh

1. Hernandez Jr., Roland. Detection of escherichia coli in northern Lake Michigan waters using qpcr method c.

Degree: 2018, University of Wisconsin – Oshkosh

"A Thesis submitted in partial fulfillment of the requirement for the degree of Master of Science in Biology/Microbiology"

The defined substrate method, Colilert® (IDEXX, Westbrook, ME), is currently the most widely used method approved by the United States Environmental Protection Agency (US EPA) to detect Escherichia coli and total coliforms in surface water. One use for the test is to determine the risk of exposure to waterborne pathogens by beach users after contact with recreational water. However, results are not available until 18 to 24 hours after sampling because the microbiological cultures need to incubate. This could mean beach goers could be exposed to contaminated water while lab testing proceeds. To help alleviate this shortcoming the US EPA has recently investigated a quantitative polymerase chain reaction (qPCR) method for use in recreational waters. This qPCR method is called Method C (US EPA, 2015) and can reduce the analysis time to four hours when identifying E. coli DNA. My overall goal was to determine how qPCR Method C compares to traditional culture-based enumeration methods at Lake Michigan beaches. I conducted a comparison of both techniques (Method C and Colilert®) at six beaches in Door, Kewaunee, and Manitowoc counties in Wisconsin throughout the 2016 and 2017 summer beach seasons. Six beaches were selected and placed in three groups: high, moderate and low mean E. coli concentrations based on Colilert® historical data since 2003. More than 30 sampling events for each beach were used to help elucidate relationships between these two methods. My work will provide critical knowledge for beach management as practitioners consider a transition to a rapid qPCR method for E. coli detection.

Advisors/Committee Members: Kleinheinz, Gregory.

Subjects/Keywords: Water quality management; Water quality; Escherichia coli

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Hernandez Jr., R. (2018). Detection of escherichia coli in northern Lake Michigan waters using qpcr method c. (Thesis). University of Wisconsin – Oshkosh. Retrieved from http://digital.library.wisc.edu/1793/78721

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Hernandez Jr., Roland. “Detection of escherichia coli in northern Lake Michigan waters using qpcr method c.” 2018. Thesis, University of Wisconsin – Oshkosh. Accessed October 19, 2018. http://digital.library.wisc.edu/1793/78721.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Hernandez Jr., Roland. “Detection of escherichia coli in northern Lake Michigan waters using qpcr method c.” 2018. Web. 19 Oct 2018.

Vancouver:

Hernandez Jr. R. Detection of escherichia coli in northern Lake Michigan waters using qpcr method c. [Internet] [Thesis]. University of Wisconsin – Oshkosh; 2018. [cited 2018 Oct 19]. Available from: http://digital.library.wisc.edu/1793/78721.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hernandez Jr. R. Detection of escherichia coli in northern Lake Michigan waters using qpcr method c. [Thesis]. University of Wisconsin – Oshkosh; 2018. Available from: http://digital.library.wisc.edu/1793/78721

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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