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University of Western Ontario

1. Burke, William. RNAi Targeting of Manduca sexta Using Chloroplast-Encoded Long dsRNA.

Degree: 2018, University of Western Ontario

Pest management strategies are crucial for reducing insect crop damage, and methods with species-specific effect are particularly desirable. Expressing dsRNA with sequence complementarity to a vital gene of feeding pests is a strategy that has shown potential. I modified the chloroplast genome of cultivated tobacco, Nicotiana tabacum, by adding a sequence encoding a 2222 base pair dsRNA complementary to the vacuolar-type H+-ATPase subunit A mRNA of Manduca sexta and demonstrated expression of the product in leaves of the plants. Although feeding this same dsRNA that was synthesized in vitro to larvae knocked down expression of the target gene and induced lethality, chloroplast-encoded dsRNA had no effect on survival or target gene expression of feeding larvae. The results of this study suggest that a possible limitation for this strategy is the level of expression due to the length of the product, or that this strategy is inefficient for certain insect species.

Subjects/Keywords: pest management; transplastomic; manduca sexta; rna interference; agriculture; nicotiana tabacum; Agriculture; Biology; Biotechnology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Burke, W. (2018). RNAi Targeting of Manduca sexta Using Chloroplast-Encoded Long dsRNA. (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/5619

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Burke, William. “RNAi Targeting of Manduca sexta Using Chloroplast-Encoded Long dsRNA.” 2018. Thesis, University of Western Ontario. Accessed December 19, 2018. https://ir.lib.uwo.ca/etd/5619.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Burke, William. “RNAi Targeting of Manduca sexta Using Chloroplast-Encoded Long dsRNA.” 2018. Web. 19 Dec 2018.

Vancouver:

Burke W. RNAi Targeting of Manduca sexta Using Chloroplast-Encoded Long dsRNA. [Internet] [Thesis]. University of Western Ontario; 2018. [cited 2018 Dec 19]. Available from: https://ir.lib.uwo.ca/etd/5619.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Burke W. RNAi Targeting of Manduca sexta Using Chloroplast-Encoded Long dsRNA. [Thesis]. University of Western Ontario; 2018. Available from: https://ir.lib.uwo.ca/etd/5619

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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