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You searched for id:"oai:etheses.bham.ac.uk:8064". One record found.

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University of Birmingham

1. Iordachescu, Alexandra. An in-vitro model for the development of mature bone containing an osteocyte network.

Degree: School of Chemical Engineering, 2018, University of Birmingham

Bone tissue continuously adapts to changes in mechanical load. This process can also result in a maladaptive ectopic bone response to mechanical insult, known as heterotopic ossification. The pathological differences at the molecular and structural levels are poorly understood. In vivo models exist but can often be too complex to allow isolation of factors which may stimulate disease progression. This thesis presents the development of a biologically self-structuring bone culture system using a fibrin gel which self-organises between two calcium phosphate anchors when seeded with cells. These bioinspired wound analogues are seeded with primary femoral periosteal cells - key players in bone repair and a range of pathologies- and develop longitudinally over time, allowing to study the temporal evolution of bone mineral and microstructure in excess of a year. Raman spectroscopy and XRD revealed that the mineral was hydroxyapatite and associated with collagen, which was organized like the in vivo tissue. The initial stem cell population differentiated to the terminal osteocytic phase, was linked by longitudinal canalicular networks (demonstrated using nanoCT) and remained viable over the year of culture. This work also demonstrated that pharmacological compounds can prevent the progress of ossification, displaying promise for applications in drug screening.

Subjects/Keywords: QP Physiology; TP Chemical technology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Iordachescu, A. (2018). An in-vitro model for the development of mature bone containing an osteocyte network. (Thesis). University of Birmingham. Retrieved from http://etheses.bham.ac.uk/8064/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Iordachescu, Alexandra. “An in-vitro model for the development of mature bone containing an osteocyte network.” 2018. Thesis, University of Birmingham. Accessed September 25, 2018. http://etheses.bham.ac.uk/8064/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Iordachescu, Alexandra. “An in-vitro model for the development of mature bone containing an osteocyte network.” 2018. Web. 25 Sep 2018.

Vancouver:

Iordachescu A. An in-vitro model for the development of mature bone containing an osteocyte network. [Internet] [Thesis]. University of Birmingham; 2018. [cited 2018 Sep 25]. Available from: http://etheses.bham.ac.uk/8064/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Iordachescu A. An in-vitro model for the development of mature bone containing an osteocyte network. [Thesis]. University of Birmingham; 2018. Available from: http://etheses.bham.ac.uk/8064/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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