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You searched for id:"oai:etd.ohiolink.edu:mco1468922731". One record found.

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University of Toledo Health Science Campus

1. Saternos, Hannah C. Newly Discovered Muscarinic Acetylcholine Receptors in the Primary Cilia.

Degree: MSP, Pharmaceutical Sciences (Pharmacology/Toxicology), 2017, University of Toledo Health Science Campus

Primary endothelial cilia are mechanosensory organelles that are projected into the lumen of blood vessels. Defects in cilia assembly or function can lead to multiple human pathologies, collectively known as ciliopathies, including polycystic kidney disease and associated hypertension. Acetylcholine, a neurotransmitter, is implicated in essential hypertension in humans and animal models. It has been demonstrated that vascular endothelia require primary cilia to sense and transmit external mechanical stimuli into internal biochemical reactions. One of these reactions includes the biosynthesis and release of nitric oxide, which is one of the most potent endogenous vasodilators. Though both primary cilia and muscarinic acetylcholine receptors play important roles in vascular hypertension, their relationship has never been explored. To determine the roles of the cholinergic system and mechanosensory cilia, we studied the effects of acetylcholine on ciliary length and function in wild-type (WT) and mechano-insensitive cilia mutant endothelial cells (Pkd1-/- and Tg737orpk/orpk). AChMR activation significantly increases cilia length in endothelial cells treated with Ach (1.49±0.006 vs 3.46±0.02 for wild-type and 0.96±0.003 vs. 3.46±0.08 for Pkd1-/-) compared to control non-treated cells. We show for the first time that not only mouse vascular endothelia exhibit muscarinic receptor-type 1, 3 and 5 (AChM1-, 3-, and 5R) co-localization to primary cilia, but also kidney epithelia exhibit muscarinic receptor-type 1 and 3 colocalization to primary cilia. Data from AChMRs localization and expression experiments clearly points towards a role of AChMRs in cell division regulation and towards their involvement in the pathogenesis in PKD. Advisors/Committee Members: AbouAlaiwi, Wissam (Committee Chair).

Subjects/Keywords: Pharmacology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Saternos, H. C. (2017). Newly Discovered Muscarinic Acetylcholine Receptors in the Primary Cilia. (Masters Thesis). University of Toledo Health Science Campus. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=mco1468922731

Chicago Manual of Style (16th Edition):

Saternos, Hannah C. “Newly Discovered Muscarinic Acetylcholine Receptors in the Primary Cilia.” 2017. Masters Thesis, University of Toledo Health Science Campus. Accessed March 30, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=mco1468922731.

MLA Handbook (7th Edition):

Saternos, Hannah C. “Newly Discovered Muscarinic Acetylcholine Receptors in the Primary Cilia.” 2017. Web. 30 Mar 2017.

Vancouver:

Saternos HC. Newly Discovered Muscarinic Acetylcholine Receptors in the Primary Cilia. [Internet] [Masters thesis]. University of Toledo Health Science Campus; 2017. [cited 2017 Mar 30]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=mco1468922731.

Council of Science Editors:

Saternos HC. Newly Discovered Muscarinic Acetylcholine Receptors in the Primary Cilia. [Masters Thesis]. University of Toledo Health Science Campus; 2017. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=mco1468922731

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