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You searched for id:"oai:escholarship.umassmed.edu:gsbs_diss-1998". One record found.

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1. Choi, Sungwook. Investigation of LIN-28 Function in Somatic Gonadal Development and Fertility, and Characterization of the LIN-28 Isoforms in C. elegans Hermaphrodites.

Degree: Interdisciplinary Graduate Program, Program in Molecular Medicine, 2018, U of Massachusetts : Med

lin-28 was first characterized as a developmental timing regulator in Caenorhabditis elegans. Loss of lin-28 function (lin-28(lf)) mutants skip the hypodermal cell fates specific to the 2nd larval stage. Here, we studied two aspects of lin-28 which had not yet been investigated. First, we show that lin-28(lf) mutants exhibit reduced fertility associated with abnormal somatic gonadal morphology. In particular, the abnormal spermatheca-uterine valve morphology of lin-28(lf) hermaphrodites traps embryos in the spermatheca, which disrupts ovulation and causes embryonic lethality. The same genes downstream of lin-28 in the regulation of hypodermal developmental timing also act downstream of lin-28 in somatic gonadal morphogenesis and fertility. Importantly, we find that hypodermal expression, but not somatic gonadal expression, of lin-28 is sufficient for restoring normal somatic gonadal morphology in lin-28(lf) mutants. We propose that the abnormal somatic gonadal morphogenesis of lin-28(lf) hermaphrodites results from temporal discoordination between the accelerated hypodermal development and normally timed somatic gonadal development. Thus, our findings exemplify how a cell-intrinsic developmental timing program can also control proper development of other interacting tissues, cell non-autonomously. We also investigated the expression patterns and functions of two lin-28 isoforms in C. elegans. Our analysis of spatial expression patterns suggests that lin-28a and lin-28b are co-expressed in diverse tissues. Consistently, neither of isoform specific knock-out mutant, lin-28a(lf) or lin-28b(lf), exhibits defects in hypodermal development, somatic gonad, or fertility, indicating functional redundancy of two isoforms. Our study will contribute to further investigation of lin-28 isoforms by providing the mutants of each isoform as well as the primary analysis of their phenotypes. Advisors/Committee Members: Victor Ambros.

Subjects/Keywords: LIN-28; C. elegans; somatic gonad; reproductive system; Developmental Biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Choi, S. (2018). Investigation of LIN-28 Function in Somatic Gonadal Development and Fertility, and Characterization of the LIN-28 Isoforms in C. elegans Hermaphrodites. (Doctoral Dissertation). U of Massachusetts : Med. Retrieved from https://escholarship.umassmed.edu/gsbs_diss/991

Chicago Manual of Style (16th Edition):

Choi, Sungwook. “Investigation of LIN-28 Function in Somatic Gonadal Development and Fertility, and Characterization of the LIN-28 Isoforms in C. elegans Hermaphrodites.” 2018. Doctoral Dissertation, U of Massachusetts : Med. Accessed November 13, 2018. https://escholarship.umassmed.edu/gsbs_diss/991.

MLA Handbook (7th Edition):

Choi, Sungwook. “Investigation of LIN-28 Function in Somatic Gonadal Development and Fertility, and Characterization of the LIN-28 Isoforms in C. elegans Hermaphrodites.” 2018. Web. 13 Nov 2018.

Vancouver:

Choi S. Investigation of LIN-28 Function in Somatic Gonadal Development and Fertility, and Characterization of the LIN-28 Isoforms in C. elegans Hermaphrodites. [Internet] [Doctoral dissertation]. U of Massachusetts : Med; 2018. [cited 2018 Nov 13]. Available from: https://escholarship.umassmed.edu/gsbs_diss/991.

Council of Science Editors:

Choi S. Investigation of LIN-28 Function in Somatic Gonadal Development and Fertility, and Characterization of the LIN-28 Isoforms in C. elegans Hermaphrodites. [Doctoral Dissertation]. U of Massachusetts : Med; 2018. Available from: https://escholarship.umassmed.edu/gsbs_diss/991

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