Advanced search options

Advanced Search Options 🞨

Browse by author name (“Author name starts with…”).

Find ETDs with:

in
/  
in
/  
in
/  
in

Written in Published in Earliest date Latest date

Sorted by

Results per page:

You searched for id:"oai:doktori.bibl.u-szeged.hu:10059". One record found.

Search Limiters

Last 2 Years | English Only

No search limiters apply to these results.

▼ Search Limiters

1. Horváth András. Investigation of antiarrhythmic and proarrhythmic mechanisms in modified systems with molecular biological methods.

Degree: 2019, University of Szeged

In cardiovascular research, there is a high need to use reliable tools for modelling cardiovascular diseases (LQT, LEOPARD, Andersen-Tawil syndrome) and for cardiovascular drug testing. It is necessary that those models reflect human heart physiology. The most commonly used models are human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) and transgenic animals, which showed promising results to study heart physiology. However, the existing hiPSC-CM and the animal models showed differences in electrophysiological properties due to immaturity and species differences. Therefore, it was of interest to study in detail the electrophysiological properties of the introduced models, hiPSC-CM and a Long QT-syndrome type 5 (LQT5) transgenic (LQT5-TG) rabbit model. The major results described in this thesis can be summarized as follows: 1. When studying the electrophysiological profile of hiPSC-CM, one of the major concerns is the low inward rectifier potassium current (IK1) and resting membrane potential (RMP). In our study, hiPSC-CM were cultivated in two different conditions. One was the conventional monolayer (ML), while the other was Engineered Heart Tissues (EHT). In our hiPSC-CM cell line we were able to measure IK1 comparable to human adult cardiomyocytes (CM). 2. In contrast to previous publications, we were able to measure resting membrane potential (RMP) values in a physiological range using sharp microelectrode in intact EHT. We showed that the low RMP measured by patch electrodes may be prone to error. 3. Patch-clamp experiments on ventricular myocytes isolated from LQT5-TG rabbits were performed. The results showed differences in IKs and IKr deactivation kinetics. The deactivation kinetics of the currents were accelerated. 4. At baseline, LQT5-TG animals showed slightly, but significantly prolonged heart-rate corrected QT index (QTi) and increased STVQT. Dofetilide provoked Torsades de Pointes (TdP) arrhythmia in a larger number of LQT5-TG rabbits, paralleled by a further increase in short-term QT-interval variability (STVQT).

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

András, H. (2019). Investigation of antiarrhythmic and proarrhythmic mechanisms in modified systems with molecular biological methods. (Thesis). University of Szeged. Retrieved from http://doktori.bibl.u-szeged.hu/10059/1/Thesis_Andras_Horvath_final.pdf ; http://doktori.bibl.u-szeged.hu/10059/2/PhdThesis_fuzet_angol.pdf ; http://doktori.bibl.u-szeged.hu/10059/3/PhdThesis_fuzet_magyar.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

András, Horváth. “Investigation of antiarrhythmic and proarrhythmic mechanisms in modified systems with molecular biological methods.” 2019. Thesis, University of Szeged. Accessed April 22, 2019. http://doktori.bibl.u-szeged.hu/10059/1/Thesis_Andras_Horvath_final.pdf ; http://doktori.bibl.u-szeged.hu/10059/2/PhdThesis_fuzet_angol.pdf ; http://doktori.bibl.u-szeged.hu/10059/3/PhdThesis_fuzet_magyar.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

András, Horváth. “Investigation of antiarrhythmic and proarrhythmic mechanisms in modified systems with molecular biological methods.” 2019. Web. 22 Apr 2019.

Vancouver:

András H. Investigation of antiarrhythmic and proarrhythmic mechanisms in modified systems with molecular biological methods. [Internet] [Thesis]. University of Szeged; 2019. [cited 2019 Apr 22]. Available from: http://doktori.bibl.u-szeged.hu/10059/1/Thesis_Andras_Horvath_final.pdf ; http://doktori.bibl.u-szeged.hu/10059/2/PhdThesis_fuzet_angol.pdf ; http://doktori.bibl.u-szeged.hu/10059/3/PhdThesis_fuzet_magyar.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

András H. Investigation of antiarrhythmic and proarrhythmic mechanisms in modified systems with molecular biological methods. [Thesis]. University of Szeged; 2019. Available from: http://doktori.bibl.u-szeged.hu/10059/1/Thesis_Andras_Horvath_final.pdf ; http://doktori.bibl.u-szeged.hu/10059/2/PhdThesis_fuzet_angol.pdf ; http://doktori.bibl.u-szeged.hu/10059/3/PhdThesis_fuzet_magyar.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.