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University of Texas – Austin

1. Ghorashian, Navid. Automated microfluidic platforms to facilitate nerve degeneration studies with C. elegans.

Degree: Biomedical Engineering, 2017, University of Texas – Austin

With its well-characterized genome, simple anatomy, and vast array of uses in molecular biology, the roundworm, Caenorhabditis elegans (C. elegans) is a well-established model organism in neurobiology. Concurrently, neurodegenerative diseases are some the most devastating and least understood ailments in modern medicine, making high-throughput approaches to understand their fundamental mechanisms imperative to developing new therapies. The worm's physical length-scales and simple genetics make it an ideal in vivo tool for high-throughput screening platforms. Concurrently, microfluidic technology has been used to make devices that manipulate these animals in a multitude of fashions to study various biological phenomena. With these considerations in mind, we have developed microfluidic platforms to facilitate optical interrogation of neurodegenerative and neuroregenerative phenomena in C. elegans for large-scale screens. First we developed a multiwell format device with 16 on-chip reservoirs to house and quickly deliver distinct worm populations to any liquid-format imaging platform. The system achieved unprecedented delivery speeds, avoided any population cross-contamination, and maintained animal viability. We then expanded this platform into a 64-well device that acted as a modular plug and play system for simple manipulation by conventional high-throughput liquid handling systems. The chip could be manipulated in the same fashion as a multiwell plate and interfaced with a novel pneumatic gasket system to achieve delivery speeds that were two-fold faster than those attained on the 16-well device. In addition, we worked to develop potential optical interrogation platform that could be fed populations of worms by the aforementioned delivery systems. This microfluidic chip consisted of an array of parallel traps to house individual worms over long durations for time-lapse studies of nerve regeneration after cuts to single axons mediated by a femtosecond pulse laser. Specifically, the platform was designed for regeneration studies in the C. elegans PQR neuron. Advisors/Committee Members: Ben-Yakar, Adela (advisor), Pierce-Shimomura, Jonathan T (committee member), Zhang, Xiaojing (committee member), Sokolov, Konstantin V (committee member), Dunn, Andrew K (committee member).

Subjects/Keywords: Neurobiology; Microfabrication; Microfluidics; Lasers; High-throughput screening

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Ghorashian, N. (2017). Automated microfluidic platforms to facilitate nerve degeneration studies with C. elegans. (Thesis). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/46483

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Ghorashian, Navid. “Automated microfluidic platforms to facilitate nerve degeneration studies with C. elegans.” 2017. Thesis, University of Texas – Austin. Accessed August 22, 2017. http://hdl.handle.net/2152/46483.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Ghorashian, Navid. “Automated microfluidic platforms to facilitate nerve degeneration studies with C. elegans.” 2017. Web. 22 Aug 2017.

Vancouver:

Ghorashian N. Automated microfluidic platforms to facilitate nerve degeneration studies with C. elegans. [Internet] [Thesis]. University of Texas – Austin; 2017. [cited 2017 Aug 22]. Available from: http://hdl.handle.net/2152/46483.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ghorashian N. Automated microfluidic platforms to facilitate nerve degeneration studies with C. elegans. [Thesis]. University of Texas – Austin; 2017. Available from: http://hdl.handle.net/2152/46483

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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