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You searched for id:"handle:1794/24545". One record found.

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University of Oregon

1. Ji, Huiying. Local Nucleic Acid Base Conformation Study by Guanine Fluorescent Analogue 6- Methyl Isoxanthopterin (6-MI) Labeled DNA.

Degree: 2019, University of Oregon

Understanding the local conformations of DNA at the level of individual nucleic acid bases is important for the study of the mechanism of DNA sequence-dependent behavior. Here we apply linear absorption, circular dichroism (CD), and fluorescence spectroscopy to study the DNA local base conformation using 6-methyl Isoxanthopterin (6-MI) labeled DNA. We interpret excitation–emission peak shift (EES) measurements of the 6-MI, both as a ribonucleotide monophosphate in solution and as a site-specific substituent for guanine in various DNA constructs, by implementing a simple two-state model. We show that the spectroscopic properties of the 6-MI probe in DNA can be used to obtain detailed information about local base conformations and conformational heterogeneity and fluctuations. Based on these findings, we apply a simple theoretical model to calculate CD of 6-MI substituted DNA constructs. We find that our model can be used to extract basesequence- dependent information about the local conformation of the 6-MI probe as modulated by the local base or base-pair environment. We next apply 6-MI to probe the ligand insertion of small molecules to duplex DNA, further extending the potential of 6- MI as a useful reporter of local nucleic acid base conformation. These studies served to establish a new level of sophistication in qualitatively analyzing 6-MI structural behavior in terms of local base stacking and unstacking conformations. v This dissertation contains previously published and unpublished co-authored material. Advisors/Committee Members: Marcud, Andrew (advisor).

Subjects/Keywords: Fluorescence spectroscopy; Local base conformation of DNA; Theoretical circular dichroism calculation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Ji, H. (2019). Local Nucleic Acid Base Conformation Study by Guanine Fluorescent Analogue 6- Methyl Isoxanthopterin (6-MI) Labeled DNA. (Thesis). University of Oregon. Retrieved from http://hdl.handle.net/1794/24545

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Ji, Huiying. “Local Nucleic Acid Base Conformation Study by Guanine Fluorescent Analogue 6- Methyl Isoxanthopterin (6-MI) Labeled DNA.” 2019. Thesis, University of Oregon. Accessed May 25, 2019. http://hdl.handle.net/1794/24545.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Ji, Huiying. “Local Nucleic Acid Base Conformation Study by Guanine Fluorescent Analogue 6- Methyl Isoxanthopterin (6-MI) Labeled DNA.” 2019. Web. 25 May 2019.

Vancouver:

Ji H. Local Nucleic Acid Base Conformation Study by Guanine Fluorescent Analogue 6- Methyl Isoxanthopterin (6-MI) Labeled DNA. [Internet] [Thesis]. University of Oregon; 2019. [cited 2019 May 25]. Available from: http://hdl.handle.net/1794/24545.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ji H. Local Nucleic Acid Base Conformation Study by Guanine Fluorescent Analogue 6- Methyl Isoxanthopterin (6-MI) Labeled DNA. [Thesis]. University of Oregon; 2019. Available from: http://hdl.handle.net/1794/24545

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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