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You searched for id:"handle:1794/24524". One record found.

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University of Oregon

1. Logan, Savannah. Imaging Vibrio Cholerae Invasion and Developing New Tools for 3D Microscopy of Live Animals.

Degree: 2019, University of Oregon

All animals harbor microorganisms that interact with each other and with their hosts. These microorganisms play important roles in health, disease, and defense against pathogens. The microbial communities in the intestine are particularly important in preventing colonization by pathogens; however, this defense mechanism and the means by which pathogens overcome it remain largely unknown. Moreover, while the composition of animal-associated microbial communities has been studied in great depth, the spatial and temporal dynamics of these communities has only recently begun to be explored. Here, we use a transparent model organism, larval zebrafish, to study how a human pathogen, Vibrio cholerae, invades intestinal communities. We pay particular attention to a bacterial competition mechanism, the type VI secrection system (T6SS), in this process. In vivo 3D fluorescence imaging and differential contrast imaging of transparent host tissue allow us to establish that V. cholerae can use the T6SS to modulate the intestinal mechanics of its host to displace established bacterial communities, and we demonstrate that one part of the T6SS apparatus, the actin crosslinking domain, is responsible for this function. Next, we develop an automated high-throughput light sheet fluorescence microscope to allow rapid imaging of bacterial communities and host cells in live larval zebrafish. Light sheet fluorescence microscopy (LSFM) has been limited in the past by low throughput and tedious sample preparation, and our new microscope features an integrated fluidic circuit and automated positioning and imaging to address these issues and allow faster collection of larger datasets, which will considerably expand the use of LSFM in the life sciences. This microscope could also be used for future experiments related to bacterial communities and the immune system. The overarching theme of the work in this dissertation is the use and development of advanced imaging techniques to make new biological discoveries, and the conclusions of this work point the way toward understanding pathogenic invasion, maximizing the use of LSFM in the life sciences, and gaining a better grasp of host-associated bacterial community dynamics. This dissertation includes previously published and unpublished co-authored material. Advisors/Committee Members: Parthasarathy, Raghuveer (advisor).

Subjects/Keywords: Host-microbe interactions; Instrument design; Light sheet fluorescence microscopy; Pathogens

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Logan, S. (2019). Imaging Vibrio Cholerae Invasion and Developing New Tools for 3D Microscopy of Live Animals. (Thesis). University of Oregon. Retrieved from http://hdl.handle.net/1794/24524

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Logan, Savannah. “Imaging Vibrio Cholerae Invasion and Developing New Tools for 3D Microscopy of Live Animals.” 2019. Thesis, University of Oregon. Accessed May 25, 2019. http://hdl.handle.net/1794/24524.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Logan, Savannah. “Imaging Vibrio Cholerae Invasion and Developing New Tools for 3D Microscopy of Live Animals.” 2019. Web. 25 May 2019.

Vancouver:

Logan S. Imaging Vibrio Cholerae Invasion and Developing New Tools for 3D Microscopy of Live Animals. [Internet] [Thesis]. University of Oregon; 2019. [cited 2019 May 25]. Available from: http://hdl.handle.net/1794/24524.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Logan S. Imaging Vibrio Cholerae Invasion and Developing New Tools for 3D Microscopy of Live Animals. [Thesis]. University of Oregon; 2019. Available from: http://hdl.handle.net/1794/24524

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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