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Brno University of Technology

1. Juglová, Zuzana. Vývoj metody pro stanovení antibiotik v mléce pomocí vysokoúčinné kapalinové chromatografie: Development of a method for determination of antibiotics in milk using high performance liquid chromatography.

Degree: 2020, Brno University of Technology

This diploma thesis focuses on optimization and validation of the method for a mixture of tetracycline antibiotics (oxytetracycline, tetracycline) determination using high performance liquid chromatography with a diode array detector (HPLC–DAD). The aim of this thesis was to determine suitability of the system for the determination of these antibiotics in milk. The theoretical part is focused on individual groups of antibiotics and their mechanism of action, resistances and possibilities of use. Next point of this thesis is legislation, which describes permitted amounts of antibiotics in milk. In this study, the HPLC method and validation parameter is described in detail. The experimental part deals with creation and optimization of the method and its validation. Following validation parameters were verified: system suitability, linearity, method accuracy, limit of detection and quantification, robustness. The results obtained indicate mostly suitable ratio of mobile phase of 0,01 M trifluoracetic acid:methanol:acetonitrile = 80:10:10. The wavelength 355 nm was found as the most feasible for this method after evaluating the result. Determination of robustness showed that the method is robust, and the only significant effect on the robustness was observed in column changes. Advisors/Committee Members: Diviš, Pavel (advisor), Pořízka, Jaromír (referee).

Subjects/Keywords: tetracyklinová antibiotika; oxytetracyklin; tetracyklin validace metody; HPLC-DAD; Tetracicline antibiotics; oxytetracycline; tetracycline; method validation; HPLC-DAD

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Juglová, Z. (2020). Vývoj metody pro stanovení antibiotik v mléce pomocí vysokoúčinné kapalinové chromatografie: Development of a method for determination of antibiotics in milk using high performance liquid chromatography. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/190409

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Juglová, Zuzana. “Vývoj metody pro stanovení antibiotik v mléce pomocí vysokoúčinné kapalinové chromatografie: Development of a method for determination of antibiotics in milk using high performance liquid chromatography.” 2020. Thesis, Brno University of Technology. Accessed August 11, 2020. http://hdl.handle.net/11012/190409.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Juglová, Zuzana. “Vývoj metody pro stanovení antibiotik v mléce pomocí vysokoúčinné kapalinové chromatografie: Development of a method for determination of antibiotics in milk using high performance liquid chromatography.” 2020. Web. 11 Aug 2020.

Vancouver:

Juglová Z. Vývoj metody pro stanovení antibiotik v mléce pomocí vysokoúčinné kapalinové chromatografie: Development of a method for determination of antibiotics in milk using high performance liquid chromatography. [Internet] [Thesis]. Brno University of Technology; 2020. [cited 2020 Aug 11]. Available from: http://hdl.handle.net/11012/190409.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Juglová Z. Vývoj metody pro stanovení antibiotik v mléce pomocí vysokoúčinné kapalinové chromatografie: Development of a method for determination of antibiotics in milk using high performance liquid chromatography. [Thesis]. Brno University of Technology; 2020. Available from: http://hdl.handle.net/11012/190409

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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