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Universitat Autònoma de Barcelona

1. Pérez Jiménez, Vanessa. Aplicación de la proteómica en la búsqueda de biomarcadores de glomeruloesclerosis segmentaria y focal y nefropatía por cambios mínimos.

Degree: Departament de Medicina, 2017, Universitat Autònoma de Barcelona

Minimal change disease (MCD) and primary focal segmental glomerulosclerosis (FSGS) are glomerular diseases defined by lesions of the podocyte. These diseases are main causes of idiopathic nephrotic syndrome in children and adults. This syndrome is characterized by proteinuria, hypoalbuminemia, hyperlipidemia and edema, without an underlying etiology. The final diagnosis of glomerular diseases is based on renal biopsy findings and their correlation with the clinical, laboratory and serological results. Moreover, renal biopsy is useful for determining the prognosis and for choosing the most appropriate treatment, although the invasiveness of this technique may lead to serious complications Anatomopathologic study combines conventional light microscopy, immunohistology and electron microscopy, and requires an adequate amount of tissue, with a minimum number of glomeruli to evidence the lesion. Light microscopy shows normal glomeruli in MCD and segmental scarring in some, but not all, glomeruli in FSGS. In both entities, electron microscopy typically demonstrates specific ultrastructural findings of diffuse effacement of podocytes’ foot processes in the absence of electron-dense deposits. Due to the focal nature of FSGS, it is complicated to identify this lesion if no affected glomeruli are sampled in the biopsy, and a misdiagnosis of these patients as MCD may occur. The correct diagnosis is crucial to an effective treatment, as MCD is typically responsive to steroid therapy with excellent long-term prognosis, whereas FSGS is usually resistant to steroid therapy and has progressive glomerular filtration rate loss. Consequently, the different therapy approach and the toxicity of steroids make it especially important to differentiate between these disorders. During the last decades, major technological advances in the field of proteomics have encouraged the search for diagnostic biomarkers of diseases in biological fluids. In this study, different proteomic methodologies were used to analyze the urinary peptidome and proteome of MCD and FSGS biopsy-diagnosed patients. The peptidome was analyzed by using magnetic-beads technology combined with MALDI-TOF mass spectrometry, while the proteome was analyzed by 2D-DIGE and MALDI-TOF mass spectrometry. As a result, some proteins were identified as potential candidate biomarkers able to differentiate between these glomerular diseases. One of the candidates was alpha-1-antitrypsin. This protein was increased in glomerular patients when comparing with healthy population, and also allowed differentiating between MCD and FSGS. By 2D-DIGE, several spots identified as alpha-1-antitrypsin showed a higher volume in MCD. This protein was measured by ELISA in the urine of an independent group of patients and higher concentrations were found in those with MCD. Moreover, by studying the urinary peptidome, a peptide of alpha-1-antitrypsin showed differences between MCD and FSGS, with higher intensity in those with MCD. By studying the urinary peptidome, several peptides of… Advisors/Committee Members: [email protected] (authoremail), true (authoremailshow), Romero González, Ramón (director), true (authorsendemail).

Subjects/Keywords: Proteòmica; Proteómica; Proteomics; Síndrome nefròtica; Síndrome nefrótico; Nephrotic syndrome; Biomarcadors; Biomarcadores; Biomarkers; Ciències de la Salut; 616.6

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APA (6th Edition):

Pérez Jiménez, V. (2017). Aplicación de la proteómica en la búsqueda de biomarcadores de glomeruloesclerosis segmentaria y focal y nefropatía por cambios mínimos. (Thesis). Universitat Autònoma de Barcelona. Retrieved from http://hdl.handle.net/10803/402221

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Pérez Jiménez, Vanessa. “Aplicación de la proteómica en la búsqueda de biomarcadores de glomeruloesclerosis segmentaria y focal y nefropatía por cambios mínimos.” 2017. Thesis, Universitat Autònoma de Barcelona. Accessed October 21, 2017. http://hdl.handle.net/10803/402221.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Pérez Jiménez, Vanessa. “Aplicación de la proteómica en la búsqueda de biomarcadores de glomeruloesclerosis segmentaria y focal y nefropatía por cambios mínimos.” 2017. Web. 21 Oct 2017.

Vancouver:

Pérez Jiménez V. Aplicación de la proteómica en la búsqueda de biomarcadores de glomeruloesclerosis segmentaria y focal y nefropatía por cambios mínimos. [Internet] [Thesis]. Universitat Autònoma de Barcelona; 2017. [cited 2017 Oct 21]. Available from: http://hdl.handle.net/10803/402221.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pérez Jiménez V. Aplicación de la proteómica en la búsqueda de biomarcadores de glomeruloesclerosis segmentaria y focal y nefropatía por cambios mínimos. [Thesis]. Universitat Autònoma de Barcelona; 2017. Available from: http://hdl.handle.net/10803/402221

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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