Advanced search options

Advanced Search Options 🞨

Browse by author name (“Author name starts with…”).

Find ETDs with:

in
/  
in
/  
in
/  
in

Written in Published in Earliest date Latest date

Sorted by

Results per page:

You searched for id:"handle:10012/14913". One record found.

Search Limiters

Last 2 Years | English Only

No search limiters apply to these results.

▼ Search Limiters


University of Waterloo

1. Vasiljevic, Tijana. Developments and applications of solid phase microextraction (SPME) coupled to direct analysis in real time (DART) and approaches towards small volume analysis.

Degree: 2019, University of Waterloo

Solid phase microextraction (SPME) has been rapidly developing since its invention in 1990. Due to a flexible design and the ability to implement various extractive coatings, SPME has found use in numerous applications. One such application is the coupling of SPME to Direct Analysis in Real Time (DART), an ambient ionization mass spectrometry (AIMS) technique. AIMS techniques completely eliminate the use of chromatography, reducing lengthy workflows and improving time-efficiency. SPME coupled to DART-MS has been quite successful in achieving low detection limits and reproducible results in bioanalytical applications. These successes are mostly due to the excellent preconcentrating and interference resisting abilities of SPME coatings and detection capabilities of DART-MS. Certainly, as a novel technique, many aspects of SPME-DART-MS are yet to be explored. Hence, this thesis investigated fundamental aspects of SPME-DART-MS, in addition to exploring novel applications, such as detection of prohibited substances in small bio-volumes (i.e., ≤ 25 µL of oral fluid (OF) and human blood) and implementation of SPME-DART-MS as high-throughput technology for quantitation of opioids in urine and human plasma in less than 1.5 h for a set of 96 samples. Coupling of SPME to portable DART-MS instrumentation was investigated for on-site, in vivo drug detection in samples of OF as a potential tool of law enforcement, reporting detection limits that are below those proposed by regulatory agencies. Additionally, the use of biocompatible plastic materials such as polyetheretherketone (PEEK) was investigated as a potential alternative material for the manufacturing of SPME devices for DART-MS, which are usually made of stainless steel (SS). Performance of the PEEK devices was similar to those of SS, allowing for their use in quantification of drugs-of-abuse (DoAs) in OF and urine at low parts-per-billion levels (ppb). In addition, OF samples following coffee consumption from a female volunteer were used to monitor caffeine half-life. The results were in compliance with established half-life of caffeine reported in literature, and highlighted the potential of the PEEK devices for in vivo buccal swabbing testing. Another novel application of SPME described in this thesis was the development of miniaturized SPME devices “minitips” for analysis of small volumes. The analysis of small sample volumes (biofluids, tissues, and cells) has become more common within the scientific community in the last few years. For this type of analysis, very sensitive instrumental platforms and sample preparation methods are required to obtain qualitative and quantitative information. Hence, the SPME minitip format was introduced, and the tip apex was coated with 1 millimeter (mm) of polyacrylonitrile (PAN) and in-house-synthesized N-vinylpyrrolidone-co-divinylbenzene, also known as hydrophilic lipophilic balance (HLB) particles. Robustness, extraction efficiency, repeatability, reusability, and matrix effect (ME) assessment yielded good results.…

Subjects/Keywords: Sample preparation; Solid phase microextraction; Bioanalysis; Drug analysis; Direct analysis in real time; Small volumes; Metabolomics

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Vasiljevic, T. (2019). Developments and applications of solid phase microextraction (SPME) coupled to direct analysis in real time (DART) and approaches towards small volume analysis. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/14913

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Vasiljevic, Tijana. “Developments and applications of solid phase microextraction (SPME) coupled to direct analysis in real time (DART) and approaches towards small volume analysis.” 2019. Thesis, University of Waterloo. Accessed September 19, 2019. http://hdl.handle.net/10012/14913.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Vasiljevic, Tijana. “Developments and applications of solid phase microextraction (SPME) coupled to direct analysis in real time (DART) and approaches towards small volume analysis.” 2019. Web. 19 Sep 2019.

Vancouver:

Vasiljevic T. Developments and applications of solid phase microextraction (SPME) coupled to direct analysis in real time (DART) and approaches towards small volume analysis. [Internet] [Thesis]. University of Waterloo; 2019. [cited 2019 Sep 19]. Available from: http://hdl.handle.net/10012/14913.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Vasiljevic T. Developments and applications of solid phase microextraction (SPME) coupled to direct analysis in real time (DART) and approaches towards small volume analysis. [Thesis]. University of Waterloo; 2019. Available from: http://hdl.handle.net/10012/14913

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.