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You searched for +publisher:"Washington State University" +contributor:("Mealey, Robert H"). Showing records 1 – 3 of 3 total matches.

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Washington State University

1. [No author]. Neutralizing antibody-mediated control of lentivirus infection .

Degree: 2010, Washington State University

NEUTRALIZING ANTIBODY-MEDIATED CONTROL OF LENTIVIRUS INFECTIONAbstractby Sandra D. Taylor, Ph.D.Washington State UniversityDecember 2010Chair: Robert H. MealeyVaccines preventing HIV-1 infection will likely elicit antibodies that neutralize diverse strains. However, the capacity for lentiviruses to escape broadly neutralizing antibodies (nAbs) is not completely understood, nor is it known whether nAbs alone can control heterologous infection. In the first experiment, we determined that convalescent immune plasma from a horse (A2150) persistently infected with equine infectious anemia virus (EIAV) neutralized homologous virus and several envelope variants containing heterologous principal neutralizing domains (PND). Plasma was infused into foals affected with severe combined immunodeficiency (SCID), followed by challenge with a homologous EIAV stock. Treated SCID foals were protected against clinical disease, with complete prevention of infection occurring in one foal. In three SCID foals, a novel neutralization-resistant variant, designated EIAVWSU5-V55, arose that was found to pre-exist at a low frequency in the challenge inoculum. In contrast, SCID foals infused with non-immune plasma developed acute disease associated with high levels of the predominant challenge virus. Following transfer to an immunocompetent horse, EIAVWSU5-V55 induced a single febrile episode and was subsequently controlled in the absence of type-specific nAb. Long term control was associated with the presence of cytotoxic T lymphocytes (CTL). The goal of the second study was to determine if purified plasma immunoglobulin (PPIG) with more potent and broadly neutralizing activity than the plasma used in the first study would prevent homologous and heterologous infection in SCID foals. Thus, plasma or PPIG from horse A2140 was infused into five experimental SCID foals prior to homologous EIAV challenge. Four of these foals were completely protected from infection, while one demonstrated partial protection. However, PPIG infused into an additional SCID foal followed by heterologous EIAVPND5 challenge failed to protect against infection. In the final experiment, pseudoviruses expressing specific variable regions of EIAVWSU5-V55 were tested for neutralization by PPIG from horses A2150 and A2140 to identify SU regions that contribute to neutralization resistance. Our results suggest that although high-titered, broadly nAb can prevent homologous infection in the absence of CTL, they may not be sufficient to prevent heterologous infection. Therefore, a protective lentivirus vaccine will likely require immunogens that elicit broadly nAb and CTL. Advisors/Committee Members: Mealey, Robert H (advisor).

Subjects/Keywords: antibody; EIAV; neutralization; resistance; SCID

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

author], [. (2010). Neutralizing antibody-mediated control of lentivirus infection . (Thesis). Washington State University. Retrieved from http://hdl.handle.net/2376/2791

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

author], [No. “Neutralizing antibody-mediated control of lentivirus infection .” 2010. Thesis, Washington State University. Accessed December 06, 2019. http://hdl.handle.net/2376/2791.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

author], [No. “Neutralizing antibody-mediated control of lentivirus infection .” 2010. Web. 06 Dec 2019.

Vancouver:

author] [. Neutralizing antibody-mediated control of lentivirus infection . [Internet] [Thesis]. Washington State University; 2010. [cited 2019 Dec 06]. Available from: http://hdl.handle.net/2376/2791.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

author] [. Neutralizing antibody-mediated control of lentivirus infection . [Thesis]. Washington State University; 2010. Available from: http://hdl.handle.net/2376/2791

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


Washington State University

2. [No author]. IN VITRO DRUG SUSCEPTIBILITY OF THEILERIA EQUI AND THE ROLE OF ABC TRANSPORTERS IN RESISTANCE TO IMIDOCARB DIPROPIONATE .

Degree: 2015, Washington State University

The primary work presented involves evaluation of the apicomplexan hemoparasite Theileria equi for imidocarb dipropionate resistance, assessment of possible alternative treatment options, and investigation of the potential role for ATP-binding cassette transporters in drug resistance. An in vitro drug susceptibility assay was developed and adapted to multiple different drugs. The susceptibility of two T. equi isolates and a lab generated variant to both imidocarb dipropionate and the experimental bumped kinase inhibitor 1294 was assessed. Two distinct isolates of T. equi had an almost four-fold difference in imidocarb dipropionate susceptibility, and a variant generated through continuous in vitro imidocarb dipropionate exposure developed a further 15-fold decrease in susceptibility. In contrast, there was no difference in susceptibility to bumped kinase inhibitor 1294, suggesting that these drugs may represent a possible alternative for resistant T. equi parasites. Also presented is the failure of four T. equi infected ponies to clear infection with the USDA Florida strain despite two rounds of imidocarb dipropionate treatment, a demonstration of drug resistance in vivo. This collective data raises concern for the existence and emergence of drug resistance at the parasite level. An investigation was undertaken of the 45 putative T. equi ATP-binding cassette transporters as potential mediators of imidocarb dipropionate efflux leading to drug resistance. Selection of the most likely candidate transporters based on differential transcription was accomplished using RNA sequencing. No experiments performed in this study supported imidocarb dipropionate as a substrate for efflux by the most differentially expressed ABC transporter BEWA_032300, however there are other transporters of interest and other mechanisms by which ABC transporters can contribute to drug resistance for further investigation.The described drug assay was utilized in a collaborative project to examine the effect of environmental exposures on the stability and efficacy of buparvaquone and parvaquone. T. equi was used as a model organism for Theileria parva, for which these drugs are vital in prophylaxis and treatment. It was found that air exposure causes significant drug degradation and subsequent loss of efficacy, a finding that has implications for successful treatment as well as the prevention of drug resistance development. Advisors/Committee Members: Mealey, Robert H (advisor).

Subjects/Keywords: Veterinary science; Animal diseases; Pharmaceutical sciences; ABC transporter; bumped kinase inhibitor; drug resistance; equine; imidocarb; Theileria

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

author], [. (2015). IN VITRO DRUG SUSCEPTIBILITY OF THEILERIA EQUI AND THE ROLE OF ABC TRANSPORTERS IN RESISTANCE TO IMIDOCARB DIPROPIONATE . (Thesis). Washington State University. Retrieved from http://hdl.handle.net/2376/6246

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

author], [No. “IN VITRO DRUG SUSCEPTIBILITY OF THEILERIA EQUI AND THE ROLE OF ABC TRANSPORTERS IN RESISTANCE TO IMIDOCARB DIPROPIONATE .” 2015. Thesis, Washington State University. Accessed December 06, 2019. http://hdl.handle.net/2376/6246.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

author], [No. “IN VITRO DRUG SUSCEPTIBILITY OF THEILERIA EQUI AND THE ROLE OF ABC TRANSPORTERS IN RESISTANCE TO IMIDOCARB DIPROPIONATE .” 2015. Web. 06 Dec 2019.

Vancouver:

author] [. IN VITRO DRUG SUSCEPTIBILITY OF THEILERIA EQUI AND THE ROLE OF ABC TRANSPORTERS IN RESISTANCE TO IMIDOCARB DIPROPIONATE . [Internet] [Thesis]. Washington State University; 2015. [cited 2019 Dec 06]. Available from: http://hdl.handle.net/2376/6246.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

author] [. IN VITRO DRUG SUSCEPTIBILITY OF THEILERIA EQUI AND THE ROLE OF ABC TRANSPORTERS IN RESISTANCE TO IMIDOCARB DIPROPIONATE . [Thesis]. Washington State University; 2015. Available from: http://hdl.handle.net/2376/6246

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

3. [No author]. Protective effects of an experimental sporozoite-based immunization strategy against Theileria equi .

Degree: 2016, Washington State University

Theileria equi is a tick-transmitted apicomplexan hemoparasite parasite of equids with a worldwide distribution and a cause of equine piroplasmosis, a World Organization of Animal Health (OIE) reportable disease. Although a protective vaccine would improve current T. equi control strategies, no vaccine is available. In natural transmission the infective life stage is the sporozoite, which is inoculated by ticks; therefore specific immune responses directed against sporozoites could prevent the establishment of infection. The purpose of the present study was to evaluate the protective effects of an experimental radiation-attenuated whole sporozoite immunization strategy against T. equi sporozoite challenge. Three horses were immunized with irradiated sporozoites three times at six week intervals, while three additional horses were inoculated with non-irradiated infectious sporozoites at the same interval, and cleared of infection three weeks after each inoculation by treatment with imidocarb dipropionate. All horses were subsequently challenged with non-irradiated infectious T. equi sporozoites. Repeated immunization with irradiated sporozoites prevented infection in one horse, while partial protection (mitigation of clinical signs) was observed in the two other horses. Prior to challenge, antibodies against equi merozoite antigen (EMA)-1 and 2 were identified in both groups but did not correlate with complete protection. Our results suggest that a protective sporozoite-based vaccine is feasible against T. equi, but correlates of protection are yet undefined. In addition, this study describes a method for efficiently generating a T. equi sporozoite preparation with enhanced purity. Advisors/Committee Members: Mealey, Robert H (advisor).

Subjects/Keywords: Veterinary science; Immunology; Parasitology; Equine; Theileria equi; Vaccine

…Institutional Animal Care and Use Committees at both Washington State University and University of… …months of age at the initiation of the experiment, were acquired from the Washington State… …University research breeding herd. Three horses (762, 764, 766) were repeatedly… 

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

author], [. (2016). Protective effects of an experimental sporozoite-based immunization strategy against Theileria equi . (Thesis). Washington State University. Retrieved from http://hdl.handle.net/2376/12093

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

author], [No. “Protective effects of an experimental sporozoite-based immunization strategy against Theileria equi .” 2016. Thesis, Washington State University. Accessed December 06, 2019. http://hdl.handle.net/2376/12093.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

author], [No. “Protective effects of an experimental sporozoite-based immunization strategy against Theileria equi .” 2016. Web. 06 Dec 2019.

Vancouver:

author] [. Protective effects of an experimental sporozoite-based immunization strategy against Theileria equi . [Internet] [Thesis]. Washington State University; 2016. [cited 2019 Dec 06]. Available from: http://hdl.handle.net/2376/12093.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

author] [. Protective effects of an experimental sporozoite-based immunization strategy against Theileria equi . [Thesis]. Washington State University; 2016. Available from: http://hdl.handle.net/2376/12093

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.