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You searched for +publisher:"Vanderbilt University" +contributor:("Thomas M. Aune, Ph.D."). One record found.

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Vanderbilt University

1. Spurlock III, Charles Floyd. Methotrexate and Rheumatoid Arthritis: At the Crossroads Between Inflammation and Defects in Cell Cycle Checkpoints.

Degree: PhD, Microbiology and Immunology, 2014, Vanderbilt University

Rheumatoid arthritis is the most common serious autoimmune disease affecting almost one percent of the human population worldwide. Methotrexate is the most commonly used disease-modifying agent in patients with rheumatoid arthritis. Despite decades-long experience with the use of methotrexate in this disease, the mechanisms responsible for its activity in rheumatoid arthritis are not very well understood. Through a series of biochemical approaches and in vivo studies in patients with rheumatoid arthritis, we have defined two novel pathways contributing to the anti-inflammatory effects of methotrexate in T cells. The first pathway is dependent upon blockade of tetrahydropbiopterin biosynthesis resulting in increased activation of c-Jun-N-terminal kinase, restoration of cell cycle checkpoint deficiencies, and reduced levels of nuclear factor kappa B, a master regulator of inflammation. Finally, we also discovered that methotrexate induces expression of the long, intergenic non-coding RNA, lincRNA-p21. Independent of methotrexate-mediated blockade of tetrahydrobiopterin and increased activity of c-Jun-N-terminal kinase, induction of lincRNA-p21 by methotrexate also reduces indices of inflammation via blockade of nuclear factor kappa B activity. Thus, multiple pathways are responsible for the immunomodulatory effects of methotrexate in the treatment of rheumatoid arthritis. Advisors/Committee Members: Subramaniam Sriram, M.B., B.S. (committee member), Jonathan M. Irish, Ph.D. (committee member), Amy S. Major, Ph.D. (committee member), Andrew J. Link, Ph.D. (chair), Thomas M. Aune, Ph.D. (committee member).

Subjects/Keywords: methotrexate; autoimmune disease; inflammation; rheumatoid arthritis; cell cycle checkpoints; c-Jun-N-terminal kinase; p53; tetrahydrobiopterin; long non-coding RNA

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Spurlock III, C. F. (2014). Methotrexate and Rheumatoid Arthritis: At the Crossroads Between Inflammation and Defects in Cell Cycle Checkpoints. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://etd.library.vanderbilt.edu//available/etd-03212014-091439/ ;

Chicago Manual of Style (16th Edition):

Spurlock III, Charles Floyd. “Methotrexate and Rheumatoid Arthritis: At the Crossroads Between Inflammation and Defects in Cell Cycle Checkpoints.” 2014. Doctoral Dissertation, Vanderbilt University. Accessed April 22, 2019. http://etd.library.vanderbilt.edu//available/etd-03212014-091439/ ;.

MLA Handbook (7th Edition):

Spurlock III, Charles Floyd. “Methotrexate and Rheumatoid Arthritis: At the Crossroads Between Inflammation and Defects in Cell Cycle Checkpoints.” 2014. Web. 22 Apr 2019.

Vancouver:

Spurlock III CF. Methotrexate and Rheumatoid Arthritis: At the Crossroads Between Inflammation and Defects in Cell Cycle Checkpoints. [Internet] [Doctoral dissertation]. Vanderbilt University; 2014. [cited 2019 Apr 22]. Available from: http://etd.library.vanderbilt.edu//available/etd-03212014-091439/ ;.

Council of Science Editors:

Spurlock III CF. Methotrexate and Rheumatoid Arthritis: At the Crossroads Between Inflammation and Defects in Cell Cycle Checkpoints. [Doctoral Dissertation]. Vanderbilt University; 2014. Available from: http://etd.library.vanderbilt.edu//available/etd-03212014-091439/ ;

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